28 results match your criteria prism 7500

  • Page 1 of 1

TET2 Single Nucleotide Polymorphism in Myeloid Neoplasms Among Egyptian Patients.

Indian J Hematol Blood Transfus 2020 Jan 20;36(1):91-96. Epub 2019 Aug 20.

4Clinical Pathology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt.

Acute myelogenous leukemia (AML) is a heterogeneous disease characterized by myeloid progenitor cells uncontrolled proliferation gradually replacing normal hematopoiesis. To evaluate Ten Eleven Translocation 2 gene (TET2) single nucleotide polymorphism (SNP) (rs2454206, rs34402524, rs61744960) in AML, and chronic myeloid leukemia (CML) in relation to their disease prognostic criteria. The study included 136 subjects; 52 AML, 54 CML and 30 subjects as control group matched for age and sex. Read More

View Article and Full-Text PDF
January 2020

The Prognostic Significance of TET2 Single Nucleotide Polymorphism in Egyptian Chronic Myeloid Leukemia.

Mediterr J Hematol Infect Dis 2020 1;12(1):e2020004. Epub 2020 Jan 1.

Hematology, Internal Medicine, Hematology Department, Faculty of Medicine, Taiz University, Yemen.

Background: Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm where pathogenesis is based on the oncoprotein termed BCR-ABL1.1 TET2 initiates DNA demethylation and is frequently mutated in hematological malignancies, including CML. The relation between TET2 acquisition and CML transformation and/or imatinib resistance is needed to be investigated. Read More

View Article and Full-Text PDF
January 2020

[Evaluation of the Two Different Real Time Polymerase Chain Reaction Methods Used for BK Virus (BKV) Quantification and BKV Genotype Assignment].

Mikrobiyol Bul 2019 Jul;53(3):285-296

Akdeniz University Faculty of Medicine, Department of Medical Microbiology, Antalya, Turkey.

BK virus (BKV) viral load quantification has a distinct role in the clinical control of BKV nephropathy and organ rejection among renal transplant recipients. In this study, it was aimed to compare BKV DNA measurement values performed with two different real-time polymerase chain reaction (PCR) methods and to determine BKV genotypes in renal transplant recipients. Totally, 150 clinical samples tested previously in two different laboratories (Lab-1 and Lab-2) from adult and pediatric renal transplantation patients were included in the study. Read More

View Article and Full-Text PDF

Implementation and evaluation of the Presto combined qualitative real-time assay for and in Rwanda.

Afr J Lab Med 2019 18;8(1):739. Epub 2019 Apr 18.

Institute of Tropical Medicine, Department of Clinical Sciences, STI Reference Laboratory, Antwerp, Belgium.

Background: The Presto combined qualitative real-time assay for and (Presto CT/NG PCR assay) is appealing for developing countries, because it can be used with multiple DNA extraction methods and polymerase chain reaction (PCR) platforms.

Objectives: The objective of the study was to implement and evaluate the Presto CT/NG PCR assay at the National Reference Laboratory (NRL) in Kigali, Rwanda, where no real-time PCR assays for the detection of or were available.

Methods: The Presto CT/NG PCR assay was first evaluated at the Institute of Tropical Medicine (ITM) in Antwerp, Belgium. Read More

View Article and Full-Text PDF

Two Multiplex Real-Time PCR Assays to Detect and Differentiate Acinetobacter baumannii and Non- baumannii Acinetobacter spp. Carrying blaNDM, blaOXA-23-Like, blaOXA-40-Like, blaOXA-51-Like, and blaOXA-58-Like Genes.

Authors:
Qiu Yang Yongyu Rui

PLoS One 2016 8;11(7):e0158958. Epub 2016 Jul 8.

Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Nosocomial infections caused by Acinetobacter spp. resistant to carbapenems are increasingly reported worldwide. Carbapenem-resistant Acinetobacter (CRA) is becoming a serious concern with increasing patient morbidity, mortality, and lengths of hospital stay. Read More

View Article and Full-Text PDF

In children with autoimmune thyroiditis CTLA4 and FCRL3 genes--but not PTPN22--are overexpressed when compared to adults.

Neuro Endocrinol Lett 2016 ;37(1):65-9

Background: Numerous genetic studies revealed several susceptibility genes of autoimmune thyroid diseases (AITD), including CTLA4, PTPN22 and FCRL3. These immune-modulating genes are involved in genetic background of AITD among children and adult patients. However, possible age-related differences in overexpression of these genes remain unclear. Read More

View Article and Full-Text PDF

Gene expression in the lignin biosynthesis pathway during soybean seed development.

Genet Mol Res 2013 Feb 28;12(3):2618-24. Epub 2013 Feb 28.

Laboratório Central de Sementes, Departamento de Agricultura, Universidade Federal de Lavras, Lavras, MG, Brasil.

The study of gene expression in plants is fundamental, and understanding the molecular mechanisms involved in important biological processes, such as biochemical pathways or signaling that are used or manipulated in improvement programs, are key for the production of high-quality soybean seeds. Reports related to gene expression of lignin in seeds are scarce in the literature. We studied the expression of the phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase, 4-hydroxycinnamate 3-hydroxylase, and cinnamyl alcohol dehydrogenase genes involved in lignin biosynthesis during the development of soybean (Glycine max L. Read More

View Article and Full-Text PDF
February 2013

The role of phosphoinositide 3-kinase subunits in chronic thyroiditis.

Thyroid Res 2012 Dec 21;5(1):22. Epub 2012 Dec 21.

Department of Endocrinology and Metabolic Disease, Medical University of Lodz, Polish Mother's Memorial Hospital - Research Institute, Lodz, Poland.

Unlabelled:

Background: The risk of neoplastic transformation in patients with chronic thyroiditis (Hashimoto's thyroiditis - HT) is slightly increased. Genetic background of this observation is still unclear. PI3K isoforms are linked with inflammatory and neoplastic processes, thus they appear to be interesting subjects of a research in this respect. Read More

View Article and Full-Text PDF
December 2012

Riedel's thyroiditis - a case report with genes' expression studies.

Thyroid Res 2012 Mar 7;5(1). Epub 2012 Mar 7.

Department of Endocrinology and Metabolic Diseases, Polish Mother's Memorial Hospital Research Institute, Medical University of Lodz, Rzgowska St, No, 281/289, 93-338 Lodz, Poland.

Background: Genetic background of Riedel's thyroiditis remains unknown. Herein, we describe our results of studies on genes expression levels in Riedel's thyroiditis.

Case Report And Genetic Findings: We report the case of 48-year old woman with Riedel's thyroiditis who has presented unusual course of disease with non-specific cervical discomfort, though as with no pain and/or no compression symptoms. Read More

View Article and Full-Text PDF

Tamoxifen resistance and CYP2D6 copy numbers in breast cancer patients.

Asian Pac J Cancer Prev 2012 ;13(12):6101-4

Science and Research Tehran Branch of Islamic Azad University, Tehran Iran.

Background: Breast cancer accounts about one million from total annual ten million new diagnosed cases of neoplasia worldwide and is the main cause of death due to cancer in women. Tamoxifen is the most popular selective estrogen receptor modulator used in anti estrogen treatments. Tamoxifen must be converted into its metabolite endoxifen for biologic effects; this conversion process is catalysed by highly polymorphic cytochrome P450 2D6 (CYP2D6). Read More

View Article and Full-Text PDF

Comparison of six different specimen types for Epstein-Barr viral load quantification in peripheral blood of pediatric patients after heart transplantation or after allogeneic hematopoietic stem cell transplantation.

J Clin Virol 2012 Mar 17;53(3):186-94. Epub 2011 Dec 17.

Department of Pediatric Hematology and Oncology, University of Giessen, Germany.

Background: Epstein-Barr Virus (EBV) a gamma-herpes virus is associated with a spectrum of lymphoid and epithelial malignancies including posttransplant lymphoproliferative disorders (PTLD). EBV-load measurement has been shown to be important for the monitoring of these patients. However, in contrast to the viral quantification of human immunodeficiency virus or human hepatitis C virus, the EBV-load measurement has not been completely standardized as yet. Read More

View Article and Full-Text PDF

Interlaboratory validation of quantitative duplex real-time PCR method for screening analysis of genetically modified maize.

Shokuhin Eiseigaku Zasshi 2011 ;52(4):265-9

Analytical Science Division, National Food Research Institute, National Agriculture and Food Research Organization, Ibaraki.

To reduce the cost and time required to routinely perform the genetically modified organism (GMO) test, we developed a duplex quantitative real-time PCR method for a screening analysis simultaneously targeting an event-specific segment for GA21 and Cauliflower Mosaic Virus 35S promoter (P35S) segment [Oguchi et al., J. Food Hyg. Read More

View Article and Full-Text PDF
February 2012

Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus.

Mol Cell Probes 2011 Apr-Jun;25(2-3):101-7. Epub 2011 Mar 17.

Department of Botany and Microbiology, College of Science, King Saud University, 11451 Riyadh, Saudi Arabia.

A novel two-step, SYBR Green I based real-time RT-PCR assay was developed for detection and quantification of BCoV using ABI PRISM 7500 sequence detection system. The assay was carried out using two sets of primers designed to amplify highly conserved sequences of the nucleocapsid gene of BCoV and the internal control, bovine glyceraldehyde-3-phosphate dehydrogenase, RNA. Specific identification of both targets was elucidated by melt curve analysis, in which the BCoV amplified product generated a melt peak at 78. Read More

View Article and Full-Text PDF
September 2011

Is the PIK3CA gene expression level in FNAB washouts equivalent to that in postoperative tissue specimens of papillary thyroid carcinoma?

Neuro Endocrinol Lett 2011 ;32(1):59-63

Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, Polish Mother's Memorial Hospital - Research Institute, Lodz, Poland.

Introduction: Papillary thyroid carcinoma (PTC) is the most common malignant tumor of the thyroid gland. The pathogenesis of PTC remains still mostly enigmatic, although PI3K/PTEN/AKT pathway has been proposed to play a role in development of PTC. Moreover, the significance of genetic analysis in the material from fine-needle aspiration biopsy (FNAB) in PTC patients has recently been demonstrated. Read More

View Article and Full-Text PDF

Assessment of RET/PTC1 and RET/PTC3 rearrangements in fine-needle aspiration biopsy specimens collected from patients with Hashimoto's thyroiditis.

Thyroid Res 2011 Jan 10;4(1). Epub 2011 Jan 10.

Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, Poland.

Background: RET/PTC rearrangements are the most frequent molecular changes in papillary thyroid carcinoma (PTC). So far, 15 main RET/PTC rearrangements have been described, among which RET/PTC1 and RET/PTC3 are the most common in PTC - especially in radiation-induced tumours. RET/PTC1 and RET/PTC3 are the result of intrachromosomal paracentric inversions in chromosome 10, where RET and the activating genes (H4 and ELE1, respectively) are located. Read More

View Article and Full-Text PDF
January 2011

Increased expression of PIN1 gene in papillary thyroid carcinoma.

Thyroid Res 2011 Jan 10;4(1). Epub 2011 Jan 10.

Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, Poland.

Background: Peptidyl-prolyl cis/trans isomerase (Pin1), encoded by PIN1 gene with locus in chromosome 19p13, is an enzyme that catalytically induces conformational changes in proteins after phosphorylation on serine or threonine residues preceding proline (pSer/Thr-Pro motifs); in this way, it has an influence on protein interactions and intracellular localizations of proteins. The aim of the study were: 1) an assessment of PIN1 gene expression level in benign and malignant thyroid lesions; 2) the evaluation of possible correlations between gene expression and histopathological variants of papillary thyroid carcinoma (PTC) or tumour size, classified according to TNM classification of primary tumours (in case of PTC only); 3) the estimation of possible relationships between expression of the gene in question and patients' sex or age.

Methods: Seventy (70) tissue samples were analyzed: 32 cases of PTC, 7 cases of medullary thyroid carcinoma (MTC), 7 cases of follicular adenoma (FA), and 24 cases of nodular goitre (NG). Read More

View Article and Full-Text PDF
January 2011

COX-2 expression in papillary thyroid carcinoma (PTC) in cytological material obtained by fine needle aspiration biopsy (FNAB).

Thyroid Res 2011 Jan 10;4(1). Epub 2011 Jan 10.

Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, Polish Mother's Memorial Hospital - Research Institute, Lodz, Poland.

Background: COX-2 is an enzyme isoform that catalyses the formation of prostanoids from arachidonic acid. An increased COX-2 gene expression is believed to participate in carcinogenesis. Recent studies have shown that COX-2 up-regulation is associated with the development of numerous neoplasms, including skin, colorectal, breast, lung, stomach, pancreas and liver cancers. Read More

View Article and Full-Text PDF
January 2011

Relative quantification of PIK3CA gene expression level in fine-needle aspiration biopsy thyroid specimens collected from patients with papillary thyroid carcinoma and non-toxic goitre by real-time RT-PCR.

Thyroid Res 2010 Aug 30;3(1). Epub 2010 Aug 30.

Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, Polish Mother's Memorial Hospital - Research Institute, Lodz, Poland.

Background: Recent studies have shown that the phosphatidylinositol 3-kinase (PI3K) signaling pathway is important regulator of many cellular events, including apoptosis, proliferation and motility. PI3K pathway alterations (PIK3CA gene mutations and/or amplification) have been observed in various human tumours. In the majority of diagnosed cases, mutations are localized in one of the three "hot spots" in the gene, responsible for coding catalytic subunit α of class I PI3K (PIK3CA). Read More

View Article and Full-Text PDF

Down-regulation of telomerase activity and activation of caspase-3 are responsible for Tanshinone I-induced apoptosis in monocyte leukemia cells in vitro.

Int J Mol Sci 2010 May 26;11(6):2267-80. Epub 2010 May 26.

Hematological Department & Institute, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, China; E-Mails: (X.-D.L.); (R.-F.F.); (Z.-G.F.); (D.-J.L.); (R.-Z.X.); (R.-W.H.).

Tanshinone I (Tan-I) is a diterpene quinone extracted from the traditional herbal medicine Salvia miltiorrhiza Bunge. Recently, Tan-I has been reported to have anti-tumor effects. In this study, we investigated the growth inhibition and apoptosis inducing effects of Tan-I on three kinds of monocytic leukemia cells (U937, THP-1 and SHI 1). Read More

View Article and Full-Text PDF

Analysis of correlation between the process of thyroid fibrosis and TGFB1 gene expression level in fine-needle aspiration biopsy (FNAB) thyroid specimens collected from patients with Hashimoto's thyroiditis and non-toxic goitre.

Exp Clin Endocrinol Diabetes 2010 Jul 26;118(7):420-6. Epub 2010 Feb 26.

1Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, Lodz, Poland.

Transforming growth factor beta 1 (TGFB1) stimulates the production of various extracellular matrix components; at the same time, it inhibits matrix degradation. These actions of TGFB1 contribute to tissue repair, however, an altered expression of TGFB1 can be a causative factor of fibrosis processes, including thyroid fibrosis which follows chronic thyroiditis. The aim of our study was to examine a potential correlation between TGFB1 gene expression level in fine-needle aspiration biopsy (FNAB) thyroid specimens and fibrosis of the thyroid gland in two types of thyroid lesions. Read More

View Article and Full-Text PDF

[Development of new software (GiMlet) to analyze real-time PCR data based on TaqMan Chemistry and examination of factors affecting variance of the Ct values].

Shokuhin Eiseigaku Zasshi 2009 Oct;50(5):208-15

National Institute of Health Sciences, Tokyo, Japan.

The primary data obtained from real-time PCR is the fluorescence intensity. We developed new software, GiMlet, to analyze the fluorescence data more effectively. To examine factors affecting the variance of the Ct values calculated from the fluorescence data, we conducted measurements of the same test solutions containing defined numbers of copies of the targeted DNA sequence using three kinds of real-time PCR equipment, namely, ABI PRISM 7500, 7700 and 7900HT, under consistent conditions, and analyzed the obtained fluorescence data using GiMlet. Read More

View Article and Full-Text PDF
October 2009

Development of a spreadsheet for SNPs typing using Microsoft EXCEL.

Leg Med (Tokyo) 2009 Apr 3;11 Suppl 1:S453-4. Epub 2009 Mar 3.

Division of Forensic Medicine, Department of Public Health and Forensic Medicine, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Sendai, Japan.

Single-nucleotide polymorphisms (SNPs) have some characteristics that make them very appropriate for forensic studies and applications. In our institute, SNPs typings were performed by the TaqMan SNP Genotyping Assays using the ABI PRISM 7500 FAST Real-Time PCR System (AppliedBiosystems) and Sequence Detection Software ver.1. Read More

View Article and Full-Text PDF

The development of a qualitative real-time RT-PCR assay for the detection of hepatitis C virus.

Eur J Clin Microbiol Infect Dis 2008 Dec 13;27(12):1177-82. Epub 2008 Jun 13.

Department of Clinical Microbiology, St. James's Hospital, Dublin 8, Ireland.

Real-time polymerase chain reaction (PCR) represents a favourable option for the detection of hepatitis C virus (HCV). A real-time reverse transcriptase PCR (RT-PCR) assay was developed as a qualitative diagnostic screening method for the detection of HCV using the ABI PRISM 7500 Sequence Detection System. The primers and probe were designed to target the 5'-untranslated region of the hepatitis C viral genome. Read More

View Article and Full-Text PDF
December 2008

Detecting PML-RARalpha transcript in acute promyelocytic leukemia using real-time quantitative RT-PCR.

Chin Med J (Engl) 2007 Oct;120(20):1803-8

Institute of Hematology, Peking University People's Hospital, Beijing 100044, China.

Background: Real-time quantitative RT-PCR (RQ-PCR) assay has become a vital tool to monitor residual disease of leukemia. However, the complexity and standardization of RQ-PCR should never be overlooked and the results should be interpreted cautiously in clinical conditions. We aimed to assess the methodology of RQ-PCR and its clinical applications in monitoring molecular kinetics of 36 newly diagnosed cases of acute promyelocytic leukemia patients with t (15; 17) from October 2004 to December 2005. Read More

View Article and Full-Text PDF
October 2007

Assessment of cyclin D1 gene expression as a prognostic factor in benign and malignant thyroid lesions.

Neuro Endocrinol Lett 2007 Aug;28(4):341-50

Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, Polish Mother's Memorial Hospital-Research Institute, Rzgowska Street No. 281/289, 93-338 Lodz, Poland.

Objective: Cyclin D1, encoded by CCND1 (cyclin D1) gene with locus in chromosome 11q13, is a protein that plays the key role in the passage through the restriction point in G1 phase of cell cycle. The aim of the study were: 1) an assessment of CCND1 gene expression level in benign and malignant thyroid lesions and 2) the evaluation of possible correlations between gene expression and the histopathological variants of papillary thyroid carcinoma (PTC), or tumour size, classified according to TNM definition of primary tumours (in case of PTC only) or patient's sex or age.

Design: Thirty five (35) tissue samples were analysed: 24 cases of PTC, 4 cases of medullary thyroid carcinoma (MTC), 4 cases of follicular adenoma (FA) and 3 cases of nodular goitre (NG). Read More

View Article and Full-Text PDF

[Detection of PML/RARalpha gene transcripts in 46 newly diagnosed acute promyelocytic leukemia patients by real-time quantitative reverse-transcription polymerase chain reaction].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2007 Feb;15(1):1-5

Institute of Hematology, People Hospital, Peking University, Beijing 100044, China.

In order to explore the application of real-time quantitative reverse-transcription polymerase chain reaction (Q-PCR) for detecting PML/RARalpha gene transcripts in patients with acute promyelocytic leukemia (APL), the bone marrow samples from 46 newly diagnosed APL patients were collected for analysis. Three plasmids containing cDNA fragments of the bcr1-, bcr3-form PML/RARalpha and ABL control gene were constructed respectively. The ABI Prism 7500 Sequence Detection System using Taqman fluorogenic probes was used to quantify target gene. Read More

View Article and Full-Text PDF
February 2007

Advanced qRT-PCR technology allows detection of the cholecystokinin 1 receptor (CCK1R) expression in human pancreas.

Pancreas 2005 Nov;31(4):325-31

Johnson & Johnson Pharmaceutical Research & Development L.L.C, San Diego, CA 92121, USA.

Objectives: To help clarify the controversy over the detection of expression of the cholecystokinin 1 receptor (CCK1R; CCKAR) in human pancreas.

Methods: Applied qRT-PCR to detect CCK1R expression using the SYBR green/Smart Cycler II and the QZyme oligonucleotide/ABI PRISM 7500 systems to detect CCK1R expressed message in highly purified cDNAs from human pancreas and other tissues. Samples of normal pancreas were obtained at operation (pancreaticoduodenectomy; Whipple's procedure) and used to ascertain the expression of CCK1R in human tissue and investigate donor individual variability in expression levels by semi-quantitative RT-PCR and scanning densitometry. Read More

View Article and Full-Text PDF
November 2005

Prism-based excitation wavelength selection for multicolor fluorescence coincidence measurements.

Appl Opt 2005 Feb;44(6):893-7

Institute for Biomedical Technology, Department of Science and Technology, University of Twente, 7500 AE Enschede, The Netherlands.

We have designed and constructed a prism setup for multiple-color confocal fluorescence experiments. The prism setup permits easy selection of any color or any combination of colors from a multicolor light source, such as a mixed-gas argon-krypton-ion laser. The selected colors emerging from the prism setup are, by design, optimally overlapped in the focus of a high-numerical-aperture objective, such as that commonly used in single-molecule fluorescence experiments. Read More

View Article and Full-Text PDF
February 2005
  • Page 1 of 1