Methods 2021 Jun 11. Epub 2021 Jun 11.
Central Laser Facility, Rutherford Appleton Laboratory, MRC-Research Complex at Harwell, Science and Technology Facilities Council, Harwell, OX11 0QX UK; National Heart and Lung Institute, Imperial College London, Sir Alexander Fleming Building, London, SW7 2AZ UK; NIHR Imperial Biomedical Research Centre, London, SW7 2AZ UK. Electronic address:
The primary cilium is a specialized plasma membrane protrusion with important receptors for signalling pathways. In polarized epithelial cells, the primary cilium assembles after the midbody remnant (MBR) encounters the centrosome at the apical surface. The membrane surrounding the MBR, namely remnant associated membrane patch (RAMP), once situated next to the centrosome, releases some of its lipid components to form a centrosome-associated membrane patch (CAMP) from which the ciliary membrane stems. Read More