Int J Mol Sci 2021 Mar 3;22(5). Epub 2021 Mar 3.
Department of Obstetrics and Gynecology, Korea University College of Medicine 73, Inchon-ro, Seongbuk-gu, Seoul 02841, Korea.
The purpose of this study is to investigate the effectiveness of sphingosine-1-phosphate (S1P) and Z-VAD-FMK (Z-VAD) as anti-apoptotic agents to preserve ovarian function and prevent tissue damage during ovarian tissue cryopreservation and transplantation. This study consisted of two steps, in vitro and in vivo. In the first step, human ovarian tissues were cryopreserved using slow-freezing media alone, S1P, or Z-VAD (control, S1P, Z-VAD group); based on the outcomes in these groups, Z-VAD was selected for subsequent xenotransplantation. Read More