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Identification and characterization of pathogenic -specific bacteriophages and polyvalent bacteriophages in piglet gut with increasing coliphage numbers after weaning.

Appl Environ Microbiol 2021 Jun 23:AEM0096621. Epub 2021 Jun 23.

Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

Post-weaning diarrhoea in pigs is mainly caused by pathogenic and is a major source of revenue loss to the livestock industry. Bacteriophages dominate the gut virome and have the potential to regulate bacterial communities and thus influence the intestinal physiology. To determine the biological characterization of intestinal coliphages, we isolated and identified the faecal coliphages of healthy pre-weaned and post-weaned piglets from Nanjing and Chuzhou pig farms. Read More

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RV lead placement - A forgotten cause of right heart failure.

Ann Med Surg (Lond) 2021 Jul 7;67:102461. Epub 2021 Jun 7.

Thomas Jefferson University, PA, USA.

Introduction: Cardiac implantable electronic devices (CIEDs) have opened new doors, improving the quality, and increasing the duration of life by providing support of heart rate, atrioventricular and interventricular synchrony, thereby preventing sudden cardiac death. Nevertheless, these devices can pose some risks to the patients, including pacemaker-mediated cardiomyopathy and endocarditis.

Case Presentation: We elucidate the case of a patient who had severe Tricuspid Regurgitation as a result of single chamber Implantable Cardioverter Defibrillator (ICD) placement which led to right heart failure (RHF). Read More

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Clinical Characteristics of COVID-19 Related Deaths in Ethiopia.

Ethiop J Health Sci 2021 Mar;31(2):223-228

University of Gondar, college of medicine and health sciences, Gondar Ethiopia.

Background: Since the occurrence of COVID-19 in the world, it has claimed nearly 1.39 million human lives in the world and more than 1500 lives in Ethiopia. The number of deaths is increasing with variable distribution in the world. Read More

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Thermal remote sensing reveals communication between volcanoes of the Klyuchevskoy Volcanic Group.

Sci Rep 2021 Jun 22;11(1):13090. Epub 2021 Jun 22.

GFZ German Research Centre for Geosciences, Telegrafenberg, 14473, Potsdam, Germany.

Volcanoes are traditionally considered isolated with an activity that is mostly independent of the surrounding, with few eruptions only (< 2%) associated with a tectonic earthquake trigger. Evidence is now increasing that volcanoes forming clusters of eruptive centers may simultaneously erupt, show unrest, or even shut-down activity. Using infrared satellite data, we detail 20 years of eruptive activity (2000-2020) at Klyuchevskoy, Bezymianny, and Tolbachik, the three active volcanoes of the Klyuchevskoy Volcanic Group (KVG), Kamchatka. Read More

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Circulating bacterial signature is linked to metabolic disease and shifts with metabolic alleviation after bariatric surgery.

Genome Med 2021 Jun 22;13(1):105. Epub 2021 Jun 22.

Medical Department III - Endocrinology, Nephrology, Rheumatology, University of Leipzig Medical Center, Leipzig, Germany.

Background: The microbiome has emerged as an environmental factor contributing to obesity and type 2 diabetes (T2D). Increasing evidence suggests links between circulating bacterial components (i.e. Read More

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Absence of fetal heart rate cycling on the intrapartum cardiotocograph (CTG) is associated with intrapartum pyrexia and lower Apgar scores.

J Matern Fetal Neonatal Med 2021 Jun 22:1-6. Epub 2021 Jun 22.

Global Academy of Medical Education and Training, London, UK.

Background: Cycling consists of alternating periods of reduced and normal fetal heart variability, reflecting changes in fetal behavioral states. Occurrence of active and quiet sleep cycles is considered to be a hallmark of fetal autonomic nervous system integrity, demonstrating healthy interaction between the parasympathetic and sympathetic nervous systems. Cycling is an overlooked feature in most international cardiotocography (CTG) guidelines. Read More

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Fish muscle processing into seafood products reduces β-parvalbumin allergenicity.

Food Chem 2021 Jun 16;364:130308. Epub 2021 Jun 16.

Institute of Physical-Chemistry Rocasolano, Spanish National Research Council, 28006 Madrid, Spain. Electronic address:

Fish is one of the eight major foods causing type-I food allergy, and the prevalence of its allergy is increasing in part due to changes in consumption habits. One of the main drivers for these changes has been the processing developments transforming the fish muscle into seafood products. Most fish allergic patients react to the Ca-binding protein β-parvalbumin (β-PV) abundant in muscle. Read More

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Shift in place of tanning bed use from tanning salons to spa, fitness, and beauty facilities: A trend perspective.

Photodermatol Photoimmunol Photomed 2021 Jun 22. Epub 2021 Jun 22.

Mannheim Institute of Public Health, Social and Preventive Medicine, Medical Faculty Mannheim, Heidelberg University, 68167, Mannheim, Germany.

While the number of tanning salons is declining, previous research described an increasing trend of using tanning beds (TBs) in fitness studios. The authors discussed that the presence of TBs in fitness studios might strengthen the misconception that use of TBs is healthy. The same is true for TBs in facilities such as swimming pools, saunas, hotels, and cosmetic salons that aim to promote well-being. Read More

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Light-Regulated Natural Fluorescence of the PCC [email protected] Composite as an Encoded Microsphere for the Detection of Multiple Biomarkers.

ACS Sens 2021 Jun 22. Epub 2021 Jun 22.

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.

The use of color-encoded microspheres for a bead-based assay has attracted increasing attention for high-throughput multiplexed bioassays. A fluorescent PCC [email protected] composite was prepared as a bead-based assay platform by a self-assembled zeolitic imidazolate framework (ZIF-8) on the surface of inactivated PCC 6803 cells. The composite fluorescence owing to the presence of pigment proteins in PCC 6803 could be gradually bleached with the prolongation of the ultraviolet light irradiation time. Read More

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Synergistic Effect of Surface Chemistry and Surface Topography Gradient on Osteogenic/Adipogenic Differentiation of hMSCs.

ACS Appl Mater Interfaces 2021 Jun 22. Epub 2021 Jun 22.

Unit of STEM, University of South Australia, Mawson Lakes 5095, Australia.

Much attention has been paid to understanding the individual effects of surface chemistry or topography on cell behavior. However, the synergistic influence of both surface chemistry and surface topography on differentiation of human mesenchymal stem cells (hMSCs) should also be addressed. Here, gold nanoparticles were immobilized in an increasing number density manner to achieve a surface topography gradient; a thin film rich in amine (-NH) or methyl (-CH) chemical groups was plasma-polymerized to adjust the surface chemistry of the outermost layer (ppAA and ppOD, respectively). Read More

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Neurocognitive Impairment after Hematopoietic Stem Cell Transplant for Hematologic Malignancies: Phenotype and Mechanisms.

Oncologist 2021 Jun 22. Epub 2021 Jun 22.

School of Nursing.

Hematopoietic stem cell transplant (HSCT) plays a central role in the treatment of hematologic cancers. With the increasing survival of patients after HSCT, survivorship issues experienced by this population have become an important outcome. Cognitive impairment is an established sequela of HSCT, with studies to date establishing its presence, associated risk factors, and clinical phenotype. Read More

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The Role of Telemedicine, In-Home Testing and Artificial Intelligence to Alleviate an Increasingly Burdened Healthcare System: Diabetic Retinopathy.

Ophthalmol Ther 2021 Jun 22. Epub 2021 Jun 22.

Chair of Ophthalmology, University of Warmia and Mazury, Zolnierska 18, 10-561, Olsztyn, Poland.

In the presence of the ever-increasing incidence of diabetes mellitus (DM), the prevalence of diabetic eye disease (DED) is also growing. Despite many improvements in diabetic care, DM remains a leading cause of visual impairment in working-age patients. So far, prevention has been the best way to protect vision. Read More

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Cytomegalovirus Latency Exacerbated small-for-size Liver Graft Injury through Activation of CCL19/CCR7 in Hepatic Stellate Cells.

Transplantation 2021 Jun 2. Epub 2021 Jun 2.

1Department of Surgery, HKU-SZH & Faculty of Medicine, The University of Hong Kong, Hong Kong, China 2AIDS Institute and Department of Microbiology, Faculty of Medicine, The University of Hong Kong, Hong Kong, China 3Institute of Immunology, School of Medicine, Zhejiang Univerisity, Hangzhou, Zhejiang, China.

Background: The interplay between cytomegalovirus (CMV) latency and graft malfunction after living donor liver transplantation (LDLT) remains poorly defined due to the complexity of clinical confounding factors. Here, we aimed to investigate the effects of CMV latency on small-for-size graft injury and to get further insight on the pathogenic role of hepatic stellate cells (HSCs) in this process.

Methods: Rat orthotopic liver transplantation with small-for-size grafts was performed in a CMV latent model developed in immunocompetent Sprague Dawley (SD) rats using Priscott strain. Read More

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Wiad Lek 2021 ;74(4):869-873


Objective: The aim: Was to investigate the status of endotoxicosis parameters in patients with chronic pancreatitis depending on the presence of a combination with type 2 diabetes mellitus, as well as their impact on the functional capacity of the pancreas.

Patients And Methods: Materials and methods: 87 outpatients with CP with concomitant type 2 diabetes and without it were examined. The main group consisted of 62 patients with CP in the phase of therapeutic exacerbation in combination with diabetes mellitus in a state of sub- or full compensation, the comparison group - 25 patients with isolated CP, the control group consisted of 30 healthy individuals. Read More

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Comparison of two glycoengineering strategies to control the fucosylation of a monoclonal antibody.

J Biotechnol 2020 20;324S:100015. Epub 2020 Feb 20.

Department of Microbiology, University of Manitoba, Winnipeg, R3T 2N2, Canada; National Institute for Bioprocessing Research & Training (NIBRT), Fosters Avenue, Dublin4, Ireland. Electronic address:

Core fucosylation of an Fc N-linked glycan affects antibody effector functions, as the absence of fucose increases the antibody dependent cell cytotoxicity (ADCC) response with increased binding to the Fcγ receptors. The work presented here compares two different approaches to incrementally reduce core fucosylation of a camelid heavy chain antibody, EG2-hFc expressed in CHO cells which targets the EGFR receptor. The first method uses a fucosyltransferase (FUT) inhibitor, 2- fluoro peracetylated fucose (2FF), which was added to cell cultures expressing the EG2-hFc antibody in increasing concentrations up to 50μM. Read More

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February 2020

Use of magnetic nanotrap particles in capturing Yersinia pestis virulence factors, nucleic acids and bacteria.

J Nanobiotechnology 2021 Jun 21;19(1):186. Epub 2021 Jun 21.

School of Systems Biology, George Mason University, Manassas, VA, 20110, USA.

Background: Many pathogens, including Yersinia pestis, cannot be consistently and reliably cultured from blood. New approaches are needed to facilitate the detection of proteins, nucleic acid and microorganisms in whole blood samples to improve downstream assay performance. Detection of biomarkers in whole blood is difficult due to the presence of host proteins that obscure standard detection mechanisms. Read More

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A Bacterial Inflammation Sensor Regulates c-di-GMP Signaling, Adhesion, and Biofilm Formation.

mBio 2021 Jun 22:e0017321. Epub 2021 Jun 22.

Institute of Molecular Biology, University of Oregon, Eugene, Oregon, USA.

Bacteria that colonize animals must overcome, or coexist, with the reactive oxygen species products of inflammation, a front-line defense of innate immunity. Among these is the neutrophilic oxidant bleach, hypochlorous acid (HOCl), a potent antimicrobial that plays a primary role in killing bacteria through nonspecific oxidation of proteins, lipids, and DNA. Here, we report that in response to increasing HOCl levels, Escherichia coli regulates biofilm production via activation of the diguanylate cyclase DgcZ. Read More

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Transformation of short-chain chlorinated paraffins and olefins with the bacterial dehalogenase LinB from Sphingobium Indicum - Kinetic models for the homologue-specific conversion of reactive and persistent material.

Chemosphere 2021 Jun 14;283:131199. Epub 2021 Jun 14.

Laboratory for Advanced Analytical Technologies, Swiss Federal Institute for Materials Science and Technology Empa, Überlandstrasse 129, 8600, Dübendorf, Switzerland.

Structure, reactivity and physico-chemical properties of polyhalogenated compounds determine their up-take, transport, bio-accumulation, transformation and toxicity and their environmental fate. In technical mixtures of chlorinated paraffins (CPs), these properties are distributed due to the presence of thousands of homologues. We hypothesized that roles of CP dehalogenation reactions, catalyzed by the haloalkane dehalogenase LinB, depend on structural properties of the substrates, e. Read More

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Vertical migration of microplastics in porous media: Multiple controlling factors under wet-dry cycling.

J Hazard Mater 2021 Jun 17;419:126413. Epub 2021 Jun 17.

School of Environment, Tsinghua University, Beijing 100084, China. Electronic address:

Terrestrial soils are not only a large reservoir for Microplastics (MPs), but also a possible entrance to the subsurface environment, posing potential risks to the subterranean habitats and groundwater. In this study, we examined the vertical transport of MPs of four polymers, i.e. Read More

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Pyrolysis temperature-dependent carbon retention and stability of biochar with participation of calcium: Implications to carbon sequestration.

Environ Pollut 2021 Jun 9;287:117566. Epub 2021 Jun 9.

School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China; China-UK Low Carbon College, Shanghai Jiao Tong University, Shanghai, 201306, China.

Converting biomass waste into biochar by slow pyrolysis with subsequent soil amendment is a prospective approach with multiple environmental benefits including soil contamination remediation, soil amelioration and carbon sequestration. This study selected cow manure as precursor to produce biochar under 300 °C, 400 °C, 500 °C and 600 °C, and a remarkable promotion of carbon (C) retention in biochar by incorporation of exogenous Ca was achieved at all investigated pyrolysis temperatures. The C retention was elevated from 49. Read More

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HCN4 current during human sinoatrial node-like action potentials.

Prog Biophys Mol Biol 2021 Jun 18. Epub 2021 Jun 18.

Department of Medical Biology, Amsterdam UMC, University of Amsterdam, Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, the Netherlands; Department of Experimental Cardiology, Amsterdam UMC, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, Amsterdam, the Netherlands.

Background: Despite the many studies carried out over the past 40 years, the contribution of the HCN4 encoded hyperpolarization-activated 'funny' current (I) to pacemaker activity in the mammalian sinoatrial node (SAN), and the human SAN in particular, is still controversial and not fully established.

Objective: To study the contribution of I to diastolic depolarization of human SAN cells and its dependence on heart rate, cAMP levels, and atrial load.

Methods: HCN4 channels were expressed in human cardiac myocyte progenitor cells (CMPCs) and HCN4 currents assessed using perforated patch-clamp in traditional voltage clamp mode and during action potential clamp with human SAN-like action potential waveforms with 500-1500 ms cycle length, in absence or presence of forskolin to mimic β-adrenergic stimulation and a -15 mV command potential offset to mimic atrial load. Read More

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Improving pest monitoring networks using a simulation-based approach to contribute to pesticide reduction.

Theor Popul Biol 2021 Jun 18. Epub 2021 Jun 18.

INRAE, Université de Toulouse, UR MIAT, F-31320 Castanet-Tolosan, France.

Conventional pest management mainly relies on the use of pesticides. However, the negative externalities of pesticides are now well known. More sustainable practices, such as Integrated Pest Management, are necessary to limit crop damage from pathogens, pests and weeds in agroecosystems. Read More

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A novel anti-inflammatory treatment for bradykinin-induced sore throat or pharyngitis.

Immun Inflamm Dis 2021 Jun 21. Epub 2021 Jun 21.

Applied Biological Laboratories, Brooklyn, New York, USA.

Background: Often thought of as a minor health concern, sore throat or pharyngitis is an important public health issue. It is one of the most common symptoms of upper respiratory diseases including COVID-19 and is a leading cause of physician visits and antibiotic prescriptions. However, few over-the-counter medications are proven to heal sore throat inflammation. Read More

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The Cannabinoids Tetrahydrocannabinol, Cannabinol, Cannabidiol, Tetrahydrocannabivarin, and 11-nor-9-carboxy-∆9-THC in Hair.

J Anal Toxicol 2021 Jun 21. Epub 2021 Jun 21.

Psychemedics Corporation, 5832 Uplander Way, Culver City, CA 90230.

The cannabinoids tetrahydrocannabinol (THC), tetrahydrocannabivarin (THCV), cannabidiol (CBD), cannabinol (CBN) and (-)-11-nor-9-carboxy-∆9-tetrahydrocannabinol (THC-COOH) were determined in 4773 hair samples. Confirmation of THC-COOH was by GC-MS/MS. Confirmation of THC, THCV, CBN and CBD was by LC-MS/MS on an AB Sciex QTRAP 6500+ LC-MS/MS. Read More

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Metabolic Syndrome and Related Inflammation, Prevalence, and Predictive Value of C-Reactive Protein in South Asian Youths.

Metab Syndr Relat Disord 2021 Jun 21. Epub 2021 Jun 21.

Centennial Medical Center, Nashville, Tennessee, USA.

Metabolic syndrome (MetS) is widely prevalent in the South Asian (SA) population. The syndrome leads to a high risk of premature atherosclerosis and diabetes. Obesity, specifically abdominal obesity, is a central pathological mechanism of disease in this population. Read More

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Singlet and Triplet State Properties and Singlet Oxygen Yield of an Amino-Acyl-Quinoxalinone Yellow Dye.

J Fluoresc 2021 Jun 21. Epub 2021 Jun 21.

School of Chemistry and Physics, University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban, 4000, South Africa.

Amino-acyl-quinoxalinone yellow dyes are cyclised analogues of the yellow azomethine dyes developed for, and still used in, silver halide colour photography. Unlike image azomethine dyes, which are rapidly deactivated in their excited states by torsion about the azomethine bond, amino-acyl-quinoxalinone dyes have an interesting photophysics because torsion is not possible due to their cyclised structure. We report results from studies on singlet and triplet state properties, and singlet oxygen yields, of the yellow dye, 7-diethylamino-3-(2,2-dimethyl-propionyl)-5-methyl-1-phenyl-1H-quinoxalin-2-one, in polar and nonpolar solvents. Read More

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Positioning the donor in a new landscape-mothers' and fathers' experiences as their adult children obtained information about the identity-release sperm donor.

Hum Reprod 2021 Jun 21. Epub 2021 Jun 21.

Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden.

Study Question: How do heterosexual parents experience identity-release donation when adult children have obtained information about their sperm donor?

Summary Answer: Adult offspring's receipt of identifying information about the sperm donor challenged the fathers' role as a parent, which was reflected in how parents positioned the donor in relation to the family.

What Is Known Already: An increasing number of countries provide access to treatment with identity-release or 'open-identity' donors. However, there is limited knowledge about how parents experience and manage the situation when adult offspring obtain identifying information about the donor and may even establish contact with him. Read More

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Cardiovascular and renal risk factors and complications associated with COVID-19.

CJC Open 2021 Jun 16. Epub 2021 Jun 16.

Institute of Cardiovascular and Medical Sciences, British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow, UK.

The current COVID-19 pandemic, caused by the SARS-CoV-2 virus (Severe Acute Respiratory Syndrome-Coronavirus-2), represents the largest medical challenge in decades. It has exposed unexpected cardiovascular vulnerabilities at all stages of the disease (pre-infection, acute phase and subsequent chronic phase). The major cardiometabolic drivers identified to have epidemiological and mechanistic associations with COVID-19 are abnormal adiposity, dysglycemia, dyslipidemia, and hypertension. Read More

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Study of quorum-sensing and genes and their dependent virulence factors in isolates from infected burn wounds.

Access Microbiol 2021 Mar 5;3(3):000211. Epub 2021 Mar 5.

Medical Microbiology & Immunology Department, Faculty of Medicine, Mansoura University, Egypt.

Background: is an opportunistic pathogen responsible for burn-wound infection. High incidence, infection severity and increasing resistance characterize -induced burn infection.

Purpose: To estimate quorum-sensing (QS)-dependent virulence factors of isolates from burn wounds and correlate it to the presence of QS genes. Read More

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Discerning the Metal Doping Effect on Surface Redox and Acidic Properties in a MoVTeNbO for Propa(e)ne Oxidation.

ACS Omega 2021 Jun 2;6(23):15279-15291. Epub 2021 Jun 2.

Instituto Mexicano del Petróleo, Dirección de Investigación en Transformación de Hidrocarburos, Eje Central Lázaro Cárdenas Norte 152, C.P. 07730 Ciudad de México, Mexico.

Adding a small quantity of K or Bi to a MoVTeNbO via impregnation with inorganic solutions modifies its surface acid and redox properties and its catalytic performance in propa(e)ne partial oxidation to acrylic acid (AA) without detriment to its pristine crystalline structure. Bi-doping encourages propane oxydehydrogenation to propene, thus enlarging the net production rate of AA up to 35% more. The easier propane activation/higher AA production over the Bi-doped catalyst is ascribed to its higher content of surface V leading to a larger amount of total V species, the isolation site effect of NbO species on V, and its higher Lewis acidity. Read More

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