234,489 results match your criteria presence absence


Dissection of quantitative trait loci in the Lachancea waltii yeast species highlights major hotspots.

G3 (Bethesda) 2021 Sep;11(9)

Université de Strasbourg, CNRS, GMGM UMR 7156, Strasbourg, France.

Dissecting the genetic basis of complex trait remains a real challenge. The budding yeast Saccharomyces cerevisiae has become a model organism for studying quantitative traits, successfully increasing our knowledge in many aspects. However, the exploration of the genotype-phenotype relationship in non-model yeast species could provide a deeper insight into the genetic basis of complex traits. Read More

View Article and Full-Text PDF
September 2021

Unique patterns of mating pheromone presence and absence could result in the ambiguous sexual behaviors of Colletotrichum species.

G3 (Bethesda) 2021 Sep;11(9)

Department of Biochemistry, Genetics & Microbiology, Forestry & Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0083, South Africa.

Colletotrichum species are known to engage in unique sexual behaviors that differ significantly from the mating strategies of other filamentous ascomycete species. For example, most ascomycete fungi require the expression of both the MAT1-1-1 and MAT1-2-1 genes to induce sexual reproduction. In contrast, all isolates of Colletotrichum harbor only the MAT1-2-1 gene and yet, are capable of recognizing suitable mating partners and producing sexual progeny. Read More

View Article and Full-Text PDF
September 2021

Systematic Review of Outcome Measures Used in Observational Studies of Adults with Eosinophilic Esophagitis.

Int Arch Allergy Immunol 2021 Sep 20:1-25. Epub 2021 Sep 20.

Institute of Social and Preventive Medicine, University of Bern, Bern, Switzerland.

Background: Over the last 20 years, diverse outcome measures have been used to evaluate the effectiveness of therapies for eosinophilic esophagitis (EoE). This systematic review aims to identify the readouts used in observational studies of topical corticosteroids, diet, and dilation in adult EoE patients.

Methods: We searched MEDLINE and Embase for prospective and retrospective studies (cohorts/case series, randomized open-label, and case-control) evaluating the use of diets, dilation, and topical corticosteroids in adults with EoE. Read More

View Article and Full-Text PDF
September 2021

TRAP transporter TakP: a key player in the resistance against selenite-induced oxidative stress in Rhodobacter sphaeroides.

Microbiol Res 2021 Aug 8;252:126828. Epub 2021 Aug 8.

Institute of Pharmacy, Pharmaceutical Microbiology, University of Greifswald, Germany; Institute of infection medicine, Kiel University, Germany. Electronic address:

Almost one-third of all proteins require metal ions as an essential component in key biological processes and approximately half of all enzymes are associated with one or more metal ions. The naturally occurring selenium is very toxic at higher levels, but few bacteria can reduce it into the less toxic insoluble elemental selenium. Selenium is required for the synthesis of selenocysteine, an essential residue involved in the active sites of various enzymes. Read More

View Article and Full-Text PDF

The effects of a simulated fMRI environment on voice intensity in individuals with Parkinson's disease hypophonia and older healthy adults.

J Commun Disord 2021 Aug 31;94:106149. Epub 2021 Aug 31.

Roxelyn and Richard Pepper Department of Communication Sciences and Disorders, Northwestern University, Evanston, IL.

Purpose: Functional magnetic resonance imaging (fMRI) has promise for understanding neural mechanisms of neurogenic speech and voice disorders. However, performing vocal tasks within the fMRI environment may not always be analogous to performance outside of the scanner. Using a mock MRI scanner, this study examines the effects of a simulated scanning environment on vowel intensity in individuals with Parkinson's disease (PD) and hypophonia and older healthy control (OHC) participants. Read More

View Article and Full-Text PDF

Lesion-level heterogeneity of radiologic progression in patients treated with pembrolizumab.

Ann Oncol 2021 Sep 17. Epub 2021 Sep 17.

Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, New York, USA. Electronic address:

Background: Disease progression is often considered a binary state reflecting presence or absence of response. However, meaningful heterogeneity between metastatic sites of a given patient may exist and may impact therapeutic outcomes. To characterize the heterogeneity of progression with immunotherapy, we evaluated lesion-level dynamics of pembrolizumab-treated patients across three tumor types. Read More

View Article and Full-Text PDF
September 2021

Potent T cell mediated anti-inflammatory role of the selective CB2 agonist lenabasum in multiple sclerosis.

Neuropathol Appl Neurobiol 2021 Sep 20. Epub 2021 Sep 20.

Laboratory of Resolution of Neuroinflammation, IRCCS Santa Lucia Foundation, Rome, Italy.

Background: Lenabasum is a synthetic cannabinoid receptor type-2 (CB2) agonist able to exert potent anti-inflammatory effects, but its role on T cells remains unknown.

Objectives: The present study was undertaken to investigate anti-inflammatory mechanisms of lenabasum in T lymphocyte subsets and its in vivo therapeutic efficacy in experimental autoimmune encephalomyelitis (EAE).

Methods: Mononuclear cells from 17 healthy subjects (HS) and 25 relapsing-remitting multiple sclerosis (RRMS) patients were activated in presence or absence of lenabasum and analysed by flow cytometry and qRT-PCR. Read More

View Article and Full-Text PDF
September 2021

(DIGSS) Determination of Intervals using Georeferenced Survey Simulation: An R package for subsurface survey.

PLoS One 2021 20;16(9):e0257386. Epub 2021 Sep 20.

Department of Anthropology, Ohio State University, Columbus, Ohio, United States of America.

Systematic survey is a crucial component of the archaeological field endeavor. In low visibility areas, systematic subsurface testing is required, most often in the form of shovel test pits or "STPs". Decisions about the interval between STPs, and the size of such units, impact significantly both the effectiveness of survey for site location and the efficiency of such prospection efforts, and yet "cookie-cutter" survey strategies are often employed without a thorough examination of their costs and benefits. Read More

View Article and Full-Text PDF
September 2021

Molecular Study of Mec-Phenol Soluble Modulin Gene in Methicillin Resistant Staphylococcus Aureus.

Clin Lab 2021 Sep;67(9)

Background: Staphylococcus aureus (S. aureus) is a main pathogen associated with different types of hospital acquired infections. There are various factors associated with the virulence of S. Read More

View Article and Full-Text PDF
September 2021

MRI Assessment of Extra-axial Findings at Pelvic Sites in a Group of Axial-SpA Patients.

Rheumatol Ther 2021 Sep 20. Epub 2021 Sep 20.

Dipartimento di Medicina e Scienze della Salute "Vincenzo Tiberio", Università degli Studi del Molise, Via Giovanni Paolo II, C/da Tappino, 86100, Campobasso, Italy.

Introduction: MRI allows a better evaluation of inflammatory and structural lesions of the sacroiliac joints and spine in patients with axial spondyloarthritis (axial SpA), so MRI plays an important role in the classification and diagnosis of axial SpA. The aim of our study was to assess extra-axial MRI findings at pelvic sites and their possible associations with clinical indices in patients with axial SpA.

Methods: Axial-SpA patients who were classified according to the ASAS criteria and consecutively attended our clinic were assessed with pelvic MRI. Read More

View Article and Full-Text PDF
September 2021

Assessment of Silicone Particle Migration Among Women Undergoing Removal or Revision of Silicone Breast Implants in the Netherlands.

JAMA Netw Open 2021 Sep 1;4(9):e2125381. Epub 2021 Sep 1.

Department of Pathology, Radboud University Medical Center, Nijmegen, the Netherlands.

Importance: Silicone breast implants have been on the market for breast augmentation or breast reconstruction for approximately 60 years but may lead to medical complications, also called breast implant illness.

Objective: To evaluate the existence of silicone gel bleed and migration over a long time period, including the period in which the newer cohesive silicone gel breast implants were used.

Design, Setting, And Participants: In this single-center case series, capsule tissue and lymph node samples were collected from women who underwent removal or revision of silicone breast implants from January 1, 1986, to August 18, 2020, and data were extracted from the pathological reports and revision of the histology if data were missing. Read More

View Article and Full-Text PDF
September 2021

Absence of SARS-CoV-2 in the air and on the surfaces within the school environment.

J Med Microbiol 2021 Sep;70(9)

West Chester University, West Chester, PA, 19383, USA.

To the best of our knowledge to date there are no scientific studies specifically investigating whether the SARS-CoV-2 virus is present in the air or on the various surfaces in the school environment. The aim of this study was to determine if SARS-CoV-2 is present on various high touch surfaces and in the air across the elementary, middle and high schools in the Chester County of Pennsylvania, USA. One hundred and fifty surface swab samples and 45 air samples were analysed for the presence of the virus. Read More

View Article and Full-Text PDF
September 2021

Influence of surfactants on biomolecular conjugation of magnetic nanoparticles.

J Biomol Struct Dyn 2021 Sep 20:1-13. Epub 2021 Sep 20.

Department of Chemistry, University of Mumbai, Vidyanagari, Mumbai, India.

Here, we report the physicochemical interaction among iron oxide nanoparticles (MNPs) and essential biomolecules, namely, serum albumin (BSA, HSA), collagen and deoxyribonucleic acid (DNA) in the presence of various cationic, anionic and non-ionic surfactants. Iron oxide nanoparticles are synthesized by the wet chemical process and are characterized by X-ray powder diffraction analysis (XRD), Fourier transform infrared spectroscopic, UV-Vis spectroscopy, scanning electron microscopy and energy-dispersive X-ray spectroscopy mapping studies . The conjugation of MNPs protein was analyzed using UV-Vis spectroscopy, fluorescence spectroscopy, circular dichroism technique and gel electrophoresis. Read More

View Article and Full-Text PDF
September 2021

Phenotypic regionalization of the vertebral column in the thorny skate Amblyraja radiata: Stability and variation.

J Anat 2021 Sep 19. Epub 2021 Sep 19.

Laboratoire de Paléontologie et Biologie Évolutive, Université du Québec à Rimouski, Rimouski, Québec, Canada.

Regionalization of the vertebral column occurred early during vertebrate evolution and has been extensively investigated in mammals. However, less data are available on vertebral regions of crown gnathostomes. This is particularly true for batoids (skates, sawfishes, guitarfishes, and rays) whose vertebral column has long been considered to be composed of the same two regions as in teleost fishes despite the presence of a synarcual. Read More

View Article and Full-Text PDF
September 2021

Genomic stability at the coding regions of the multidrug transporter gene : insights into the development of alternative drug resistance mechanisms in human leukemia cells.

Cancer Drug Resist 2020 3;3:959-979. Epub 2020 Nov 3.

Division of Oncology, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.

Aim: Despite considerable efforts to reverse clinical multidrug resistance (MDR), targeting the predominant multidrug transporter ABCB1/P-glycoprotein (P-gp) using small molecule inhibitors has been unsuccessful, possibly due to the emergence of alternative drug resistance mechanisms. However, the non-specific P-gp inhibitor cyclosporine (CsA) showed significant clinical benefits in patients with acute myeloid leukemia (AML), which likely represents the only proof-of-principle clinical trial using several generations of MDR inhibitors. Nevertheless, the mutational mechanisms that may underlie unsuccessful MDR modulation by CsA are not elucidated because of the absence of CsA-relevant cellular models. Read More

View Article and Full-Text PDF
November 2020

The classical and updated models of androgen receptor nucleocytoplasmic trafficking.

Am J Clin Exp Urol 2021 25;9(4):287-291. Epub 2021 Aug 25.

Department of Urology, University of Pittsburgh School of Medicine Pittsburgh, PA 15232, USA.

This mini-review covers the classical model of androgen receptor (AR) nucleocytoplasmic trafficking and provides an overview of new data that updates the existing paradigm. The classical model of androgen receptor trafficking involves AR translocating to the nucleus in the presence of androgens and subsequently being exported back to the cytoplasm following the withdrawal of androgens. New data challenges and updates the fate of nuclear AR. Read More

View Article and Full-Text PDF

Dissecting Monomer-Dimer Equilibrium of an RNase P Protein Provides Insight Into the Synergistic Flexibility of 5' Leader Pre-tRNA Recognition.

Front Mol Biosci 2021 3;8:730274. Epub 2021 Sep 3.

Department of Chemistry, Marquette University, Milwaukee, WI, United States.

Ribonuclease P (RNase P) is a universal RNA-protein endonuclease that catalyzes 5' precursor-tRNA (ptRNA) processing. The RNase P RNA plays the catalytic role in ptRNA processing; however, the RNase P protein is required for catalysis and interacts with the 5' leader sequence. A single P RNA and a P protein form the functional RNase P holoenzyme yet dimeric forms of bacterial RNase P can interact with non-tRNA substrates and influence bacterial cell growth. Read More

View Article and Full-Text PDF
September 2021

Rat Hepatitis E Virus: Presence in Humans in South-Western France?

Front Med (Lausanne) 2021 3;8:726363. Epub 2021 Sep 3.

Virology Laboratory, National Reference Centre of Hepatitis E Viruses, Federal Institute of Biology, University Hospital, Toulouse, France.

Hepatitis E Virus (HEV) is one of the most common causes of hepatitis worldwide, and South-Western France is a high HEV seroprevalence area. While most cases of HEV infection are associated with the species Orthohepevirus-A, several studies have reported a few cases of HEV infections due to Orthohepevirus-C (HEV-C) that usually infects rats. Most of these human cases have occurred in immunocompromised patients. Read More

View Article and Full-Text PDF
September 2021

Gastrointestinal Symptoms and Irritable Bowel Syndrome Are Associated With Female Sex and Smoking in the General Population and With Unemployment in Men.

Front Med (Lausanne) 2021 1;8:646658. Epub 2021 Sep 1.

Department of Internal Medicine, Skåne University Hospital, Lund University, Malmö, Sweden.

The influence of daily life exposure on the gastrointestinal tract is not fully understood. This study aimed to examine associations between functional gastrointestinal symptoms and sociodemographic status and lifestyle habits in the general population. The Malmö Offspring Study (MOS) included 2,648 participants from the general population who had answered a questionnaire about sociodemographic status, lifestyle habits, medical health, and self-reported irritable bowel syndrome (IBS). Read More

View Article and Full-Text PDF
September 2021

Rapid Enabling of Resistance to High D-Sorbitol Concentration and High Temperature by Microdroplet-Aided Adaptive Evolution.

Front Bioeng Biotechnol 2021 3;9:731247. Epub 2021 Sep 3.

National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China.

is important in the conversion of D-sorbitol into l-sorbose, which is an essential intermediate for industrial-scale production of vitamin C. In a previous study, the strain WSH-004 could directly produce 2-keto-l-gulonic acid (2-KLG). However, its D-sorbitol tolerance was poor compared with that of other common industrial strains, which grew well in the presence of more than 200 g/L of D-sorbitol. Read More

View Article and Full-Text PDF
September 2021

Bridging the Bridging Imidazolate in the Bimetallic Center of the Cu/Zn SOD1 and ALS.

Front Chem 2021 3;9:716438. Epub 2021 Sep 3.

Department of Medical Informatics and Biostatistics, School of Medicine, Acibadem MAA University, Istanbul, Turkey.

Metallation status of human Cu/Zn superoxide dismutase 1 (SOD1) plays a pivotal role in the pathogenesis of amyotrophic lateral sclerosis (ALS). All of the amino acids found in the bimetallic center have been associated with ALS except for two positions. H63 which forms the bridging imidazolate ion in the bimetallic center and K136 which is not directly involved in coordination but located in the bimetallic center were not reported to be mutated in any of the identified ALS cases. Read More

View Article and Full-Text PDF
September 2021

Differences in Stress and Anxiety Among Women With and Without Children in the Household During the Early Months of the COVID-19 Pandemic.

Front Public Health 2021 1;9:688462. Epub 2021 Sep 1.

Department of Nutrition and Exercise Physiology, Washington State University, Spokane, WA, United States.

The COVID-19 pandemic and resulting lockdowns have had a disproportionate impact on parents of children under 18, particularly women. Mandatory school closures and loss of childcare resulted in parents balancing work, teaching, and childcare needs. A number of studies have examined changes in mental health of parents, but to date no studies have compared the differences in stress and anxiety levels between women with and without children in the United States. Read More

View Article and Full-Text PDF
September 2021

Mental Workload and Job Satisfaction in Healthcare Workers: The Moderating Role of Job Control.

Front Public Health 2021 3;9:683388. Epub 2021 Sep 3.

Department of Occupational Health Engineering, Shoushtar Faculty of Medical Sciences, Shoushtar, Iran.

The aim of this study was to investigate the moderating role of job control in relation to mental workload and job satisfaction of healthcare workers. This cross-sectional study was carried out on 480 nurses, midwives, and administrative workers in four educational hospitals of Ardabil, Iran. Research tools were included demographic information questionnaire, NASA-TLX questionnaire, job description index (JDI) questionnaire and job control inquiry. Read More

View Article and Full-Text PDF
September 2021

Whey Protein Lipid Concentrate High in Milk Fat Globule Membrane Components Inhibit Porcine and Human Rotavirus .

Front Pediatr 2021 1;9:731005. Epub 2021 Sep 1.

Department of Food Science & Human Nutrition, University of Illinois, Urbana, IL, United States.

The milk fat globule membrane (MFMG) is a complex milk component that has been shown to inhibit rotavirus (RV) binding to cell membranes . Herein, a whey protein lipid concentrate high in MFGM components (WPLC) and whey protein concentrate (WPC; control) were screened for anti-infective activity against porcine OSU and human Wa strains of RV in both the African Green Monkey kidney (MA104) and the human colorectal adenocarcinoma (Caco-2) cell lines. Confluent cells were exposed to OSU or Wa RV in the presence of WPLC or WPC (control) at 0, 0. Read More

View Article and Full-Text PDF
September 2021

Reduced Systolic Function and Not Genetic Variants Determine Outcome in Pediatric and Adult Left Ventricular Noncompaction Cardiomyopathy.

Front Pediatr 2021 3;9:722926. Epub 2021 Sep 3.

Department of Pediatric Cardiology, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin Institute of Health, Berlin, Germany.

Left ventricular noncompaction cardiomyopathy (LVNC CMP) is a genetic cardiomyopathy. Genotype-phenotype correlation and clinical outcome of genetic variants in pediatric and adult LVNC CMP patients are still unclear. The retrospective multicenter study was conducted in unrelated index patients with LVNC CMP, diagnosed between the years 1987 and 2017, and all available family members. Read More

View Article and Full-Text PDF
September 2021

Changes of Infant- and Family-Centered Care Practices Administered to Extremely Preterm Infants During Implementation of the NIDCAP Program.

Front Pediatr 2021 3;9:718813. Epub 2021 Sep 3.

Service de Médecine et Réanimation du Nouveau-né, Centre Hospitalier Universitaire de Strasbourg, Strasbourg, France.

Many studies have evaluated the Neonatal Individualized Developmental Care and Assessment Program (NIDCAP), but few studies have assessed changes in infant- and family-centered developmental care (IFCDC) practices during its implementation. The primary objective of this single center study was to investigate the impact of the implementation of the NIDCAP program on IFCDC practices used for management of extremely preterm infants (EPIs). The secondary objective was to determine during implementation the impact of this program on the short-term medical outcomes of all EPIs hospitalized at our center. Read More

View Article and Full-Text PDF
September 2021

Antibiotic Resistance and Virulence Profiles of Strains Isolated From Different Clinical Sources.

Front Cell Infect Microbiol 2021 1;11:738223. Epub 2021 Sep 1.

ISGlobal, Hospital Clínic-Universitat de Barcelona, Barcelona, Spain.

is a Gram-negative bacterium capable of colonizing, invading, and causing infections in different anatomical sites of the human body. Its ability to evade the immune system, its increasing antimicrobial resistance and the emergence of hypervirulent pathotypes have become a major challenge in the medical field. In this study, 127 strains from different clinical sources (urine, respiratory tract or blood) were characterized for antimicrobial resistance, the presence of virulence factor genes, serum resistance, hypermucoviscosity and the ability to form biofilms. Read More

View Article and Full-Text PDF
September 2021

A Phase II Trial of the Double Epigenetic Priming Regimen Including Chidamide and Decitabine for Relapsed/Refractory Acute Myeloid Leukemia.

Front Oncol 2021 3;11:726926. Epub 2021 Sep 3.

National Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Objective: To explore the role of chidamide, decitabine plus priming regimen in the salvage treatment of relapsed/refractory acute myeloid leukemia.

Methods: A clinical trial was conducted in relapsed/refractory acute myeloid leukemia patients using chidamide, decitabine, cytarabine, idarubicin, and granulocyte-colony stimulating factor, termed CDIAG, a double epigenetic priming regimen.

Results: Thirty-five patients were recruited. Read More

View Article and Full-Text PDF
September 2021

Prognostic Factors for Overall Survival in Nasopharyngeal Cancer and Implication for TNM Staging by UICC: A Systematic Review of the Literature.

Front Oncol 2021 2;11:703995. Epub 2021 Sep 2.

Clinical Oncology Center, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China.

This study aims to identify prognostic factors in nasopharyngeal carcinoma (NPC) to improve the current 8th edition TNM classification. A systematic review of the literature reported between 2013 and 2019 in PubMed, Embase, and Scopus was conducted. Studies were included if (1) original clinical studies, (2) ≥50 NPC patients, and (3) analyses on the association between prognostic factors and overall survival. Read More

View Article and Full-Text PDF
September 2021

Analysing "tear-drop" prints of acute promyelocytic leukemia (APML): immunophenotypic prognostication of APML by FCM.

Am J Blood Res 2021 15;11(4):446-457. Epub 2021 Aug 15.

ICMR-National Institute of Pathology, Safdarjung Hospital Campus Ansari Nagar, New Delhi, India.

Introduction: Acute promyelocytic leukemia (APML), although genetically and morphologically distinct from other AML (acute myeloid leukemia) subtypes, is one of the most best responsive acute myeloid leukemia. -Conventional diagnostic methods and morphological hints often fail in the majority of the cases in the peripheral laboratories owing to resource constraints, unavailability of cytogenetic work-up, hypogranular variants, morphological mimicry by AML-monocytic and myelo-monocytic, etc. Flowcytometry (FCM), however, can be utilized as a feasible and reliable immunophenotypic diagnostic and prognostic tool for prompt identification of APML. Read More

View Article and Full-Text PDF