68 results match your criteria prepare therapeutically


Perfusion and endothelialization of engineered tissues with patterned vascular networks.

Nat Protoc 2021 Jun 24;16(6):3089-3113. Epub 2021 May 24.

Department of Bioengineering, Rice University, Houston, TX, USA.

As engineered tissues progress toward therapeutically relevant length scales and cell densities, it is critical to deliver oxygen and nutrients throughout the tissue volume via perfusion through vascular networks. Furthermore, seeding of endothelial cells within these networks can recapitulate the barrier function and vascular physiology of native blood vessels. In this protocol, we describe how to fabricate and assemble customizable open-source tissue perfusion chambers and catheterize tissue constructs inside them. Read More

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Investigating the effects of IDO1, PTGS2, and TGF-β1 overexpression on immunomodulatory properties of hTERT-MSCs and their extracellular vesicles.

Sci Rep 2021 Apr 9;11(1):7825. Epub 2021 Apr 9.

Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Khorasan Razavi, Iran.

The therapeutic potential of mesenchymal stem cells (MSCs) is out of the question. Yet, recent drawbacks have resulted in a strategic shift towards the application of MSC-derived cell-free products such as extracellular vesicles (EVs). Recent reports revealed that functional properties of MSCs, including EV secretion patterns, correlate with microenvironmental cues. Read More

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The cancer glycocalyx mediates intravascular adhesion and extravasation during metastatic dissemination.

Commun Biol 2021 Feb 26;4(1):255. Epub 2021 Feb 26.

Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

The glycocalyx on tumor cells has been recently identified as an important driver for cancer progression, possibly providing critical opportunities for treatment. Metastasis, in particular, is often the limiting step in the survival to cancer, yet our understanding of how tumor cells escape the vascular system to initiate metastatic sites remains limited. Using an in vitro model of the human microvasculature, we assess here the importance of the tumor and vascular glycocalyces during tumor cell extravasation. Read More

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February 2021

Protective Potential of Uric Acid, Folic Acid, Glutathione and Ascorbic Acid Against the Formation of Toxic Met-Myoglobin.

Protein Pept Lett 2021 ;28(3):282-289

Department of Medical Biochemistry, College of Medicine, Qassim University, Buraidah, Saudi Arabia.

Background: Myoglobin is an oxygen binding protein and its dysfunction has been associated with the pathology of several human disorders. This study was undertaken to investigation the role of hydrogen peroxide (H2O2) in the formation of met-myoglobin and the protective potential of four different reductants such as uric acid, folic acid, glutathione and ascorbic acid were also tested against met-myoglobin formation.

Methods: Human myoglobin was treated with H2O2 in-vitro in order to prepare met-myoglobin. Read More

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January 2021

Intervertebral Disc Regeneration Using Stem Cell/Growth Factor-Loaded Porous Particles with a Leaf-Stacked Structure.

Biomacromolecules 2020 12 2;21(12):4795-4805. Epub 2020 Oct 2.

Department of Nanobiomedical Science, Dankook University, Cheonan 31116, Republic of Korea.

Although biological therapies based on growth factors and transplanted cells have demonstrated some positive outcomes for intervertebral disc (IVD) regeneration, repeated injection of growth factors and cell leakage from the injection site remain considerable challenges for human therapeutic use. Herein, we prepare human bone marrow-derived mesenchymal stem cells (BMSCs) and transforming growth factor-β3 (TGF-β3)-loaded porous particles with a unique leaf-stack structural morphology (LSS particles) as a combination bioactive delivery matrix for degenerated IVD. The LSS particles are fabricated with clinically acceptable biomaterials (polycaprolactone and tetraglycol) and procedures (simple heating and cooling). Read More

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December 2020

A Unique Case of Lower Limb Soft Tissue Reconstruction with a Prefabricated Bipedicled Deep Inferior Epigastric Artery Flap.

Plast Reconstr Surg Glob Open 2020 Jul 24;8(7):e2976. Epub 2020 Jul 24.

Laboratory of Bioengineering and Prototyping of Organs and Tissues, Institute for Regenerative Medicine, Sechenov University, Moscow, Russian Federation.

Aesthetic lower-extremity reconstruction is a secondary field in lower limb reconstructive surgery. Nevertheless, it plays an important role in the final stages of patient rehabilitation after traumatic events, treatment of deformations, and oncoplastic surgery, and in unique cases of purely aesthetic reconstruction. We present a clinical case of lower limb reconstruction with a prefabricated bipedicled deep inferior epigastric artery (DIEP) flap in a young patient who underwent a massive congenital circular pigmented nevus excision surgery. Read More

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"I found it daunting": An exploration of educational needs and experiences of mental health student nurses working with children and adolescents with eating disorders.

J Psychiatr Ment Health Nurs 2020 Dec 3;27(6):678-688. Epub 2020 Mar 3.

Trinity Centre for Practice and Healthcare Innovation, School of Nursing and Midwifery, Trinity College Dublin, The University of Dublin, Dublin, Ireland.

WHAT IS KNOWN ABOUT THE SUBJECT?: A therapeutic relationship with a professional who displays an informed approach has a positive effect on outcomes for young people presenting with an eating disorder. There is a dearth of research available on the student mental health nurse experience of working with young people with an eating disorder both internationally and from the Republic of Ireland. Within this limited literature, nursing students were found to hold more negative perceptions towards this group than more experienced clinicians. Read More

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December 2020

Saturation Mutagenesis Genome Engineering of Infective ΦX174 Bacteriophage Unamplified Oligo Pools and Golden Gate Assembly.

ACS Synth Biol 2020 01 7;9(1):125-131. Epub 2020 Jan 7.

Institute for Modeling Collaboration and Innovation , University of Idaho , Moscow , Idaho 83844 , United States.

Here we present a novel protocol for the construction of saturation single-site-and massive multisite-mutant libraries of a bacteriophage. We segmented the ΦX174 genome into 14 nontoxic and nonreplicative fragments compatible with Golden Gate assembly. We next used nicking mutagenesis with oligonucleotides prepared from unamplified oligo pools with individual segments as templates to prepare near-comprehensive single-site mutagenesis libraries of genes encoding the F capsid protein (421 amino acids scanned) and G spike protein (172 amino acids scanned). Read More

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January 2020

Terminalia arjuna gum/alginate in situ gel system with prolonged retention time for ophthalmic drug delivery.

Int J Biol Macromol 2020 Jun 18;152:1056-1067. Epub 2019 Nov 18.

Department of Chemistry, University of Sargodha, Sargodha 40100, Pakistan.

Poor availability is the major barrier to accept the new smart gel system as a preferred ophthalmic solution for various eye problems. Smart gel system especially derived from natural source allows the rapid transition of ocular solution into gel form upon contact to tear solution. The present experimental scheme was intended to prepare and characterize a pH triggered in situ gelling system using moxifloxacin HCl (MOX-HCl). Read More

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Analysis of human cGAS activity and structure.

Methods Enzymol 2019 2;625:13-40. Epub 2019 May 2.

Department of Microbiology, Harvard Medical School, Boston, MA, United States; Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA, United States; Parker Institute for Cancer Immunotherapy at Dana-Farber Cancer Institute, Boston, MA, United States. Electronic address:

Cyclic GMP-AMP synthase (cGAS) is an innate immune system enzyme responsible for recognition of double-stranded DNA aberrantly localized in the cell cytosol. cGAS binds DNA and is activated to catalyze production of the nucleotide second messenger 2'-5'/3'-5' cyclic GMP-AMP (2'3' cGAMP). In spite of a major role for cGAS in the cellular immune response, a complete understanding of cGAS biology has been limited by a lack of genetic tools to rapidly screen cGAS activity, instability of human cGAS-DNA interactions in vitro, and a previous absence of structural information for the human cGAS-DNA complex. Read More

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Development and evaluation of baicalin-loaded W/O nanoemulsion for lymphatic absorption.

Pharm Dev Technol 2019 Nov 8;24(9):1155-1163. Epub 2019 Aug 8.

Department of Pharmacy, Anhui University of Chinese Medicine , Hefei , China.

Lymphatic formations that effectively eradicate the virus in the lymphatic system will be therapeutically advantagous in hepatitis B virus (HBV) infection. Lipid-based formulation is often used to deliver drug via the lymphatic system. Baicalin nanoemulsion may be a promising drug delivery system for improved treatment of HBV infection. Read More

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November 2019

Preparation, Characterization and Prevention of Auto-oxidation of Amorphous Sirolimus by Encapsulation in Polymeric Films Using Hot Melt Extrusion.

Curr Drug Deliv 2019 ;16(7):663-671

Institute of Chemical and Engineering Sciences, 1, Pesek Road Jurong Island, Singapore-627833, Singapore.

Background: Sirolimus (SIR) is a macrocyclic lactone antibiotic and used therapeutically as a potent immunosuppressant for prophylaxis of kidney transplant rejection. The development of an oral dosage form is challenging because of very poor aqueous solubility (2.6µg/ml). Read More

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February 2020

Library Preparation Using FFPE-Derived Tumor DNA for High-Throughput Hybridization-Based Targeted or Exome Sequencing.

Methods Mol Biol 2019 ;1908:19-36

Department of Pathology, University of California San Diego, La Jolla, CA, USA.

The use of next generation sequencing (NGS) to profile tumor genomes for the presence of diagnostic, prognostic, or therapeutically targetable variants is revolutionizing the practice of oncology and is increasingly utilized in clinical laboratory settings. Beginning with the isolation of DNA of sufficient quality and quantity from a tumor specimen, the creation of a library of genomic fragments representing the portion of the genome of interest, ranging from a few genes to the entire exome, is the first step required in the sequencing process. Fixed tumor tissue in the form of a tissue block is the most commonly encountered specimen for analysis in a clinical setting. Read More

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Chemical characterization of an Ayurvedic herbo-mineral preparation- Mahalaxmivilas Rasa.

J Ayurveda Integr Med 2019 Oct - Dec;10(4):262-268. Epub 2018 Nov 20.

Central Council for Research in Ayurvedic Sciences, 61-65, Institutional Area, Opp. D-Block, Janakpuri, New Delhi, India.

Background: To protect the massive trust of patient in Ayurveda, a need aroused for the researches to ascertain the quality, safety & efficacy of herbo-mineral preparations on scientific lines. The rasa-aushadhis are having qualities such as instant effectiveness, requirement in very small dosage and ample therapeutic utility. Mahalaxmi Vilas Rasa [AFI, 20:27] has been used for treatment of a variety of ailments since time immemorial. Read More

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November 2018

Synchronized release of bufadienolides in a stable Lutrol F127 based solid dispersion prepared with spray congealing.

Drug Dev Ind Pharm 2018 Nov 31;44(11):1817-1825. Epub 2018 Aug 31.

a School of Pharmacy , Ningxia Medical University , Yinchuan , PR China.

Objective: The objective of this study was to design and prepare a novel solid dispersion using spray congealing to achieve fast and synchronous dissolution of bufalin, cinobufagin, and resibufogenin, three therapeutically complementary drugs.

Methods: The solid dispersion was characterized with dissolution, X-ray diffractometry, and fourier transform infrared spectroscopy after preparation and storage for four weeks at different temperatures and relative humidity.

Results: It was found that all drugs were molecularly dispersed within matrix and had a significant enhancement (∼4-fold higher) of dissolution rate. Read More

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November 2018

Prevalence and correlates of non-medical only compared to self-defined medical and non-medical cannabis use, Canada, 2015.

Health Rep 2018 07;29(7):3-13

Special Surveys Division, Statistics Canada, Ottawa, Ontario.

Background: The Canadian federal government has committed to legalizing non-medical cannabis use by adults in 2018. Medical use was legalized in 2001; however, not all people reporting medical use have medical authorization. To prepare for monitoring the effects of the policy change, a greater understanding of the prevalence of cannabis use and the characteristics of all cannabis users is needed. Read More

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Scalable Synthesis of Biodegradable Black Mesoporous Silicon Nanoparticles for Highly Efficient Photothermal Therapy.

ACS Appl Mater Interfaces 2018 Jul 2;10(28):23529-23538. Epub 2018 Jul 2.

Department of Physics and Astronomy , University of Turku , 20014 Turku , Finland.

Porous silicon (PSi) has attracted wide interest as a potential material for various fields of nanomedicine. However, until now, the application of PSi in photothermal therapy has not been successful due to its low photothermal conversion efficiency. In the present study, biodegradable black PSi (BPSi) nanoparticles were designed and prepared via a high-yield and simple reaction. Read More

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Chemical Characterization of an Herbo-Mineral Formulation - : A Potential Tool for Quality Assurance.

Anc Sci Life 2017 Apr-Jun;36(4):207-214

Inorganic and Physical Chemistry Division, Indian Institute of Chemical Technology (CSIR), Hyderabad, Telangana, India.

Background: Herbo-mineral formulations of Ayurveda contain specified metals or minerals as composition, which have their beneficial effects on biological systems. These metals or minerals are transformed into non-toxic forms through meticulous procedures explained in Ayurveda. Though literature is available on quality aspects of such herbo-mineral formulations; contemporary science is raising concerns at regular intervals on such formulations. Read More

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December 2017

Oral Delivery of Nanoparticles Loaded With Ginger Active Compound, 6-Shogaol, Attenuates Ulcerative Colitis and Promotes Wound Healing in a Murine Model of Ulcerative Colitis.

J Crohns Colitis 2018 Jan;12(2):217-229

Institute for Biomedical Sciences, Georgia State University, Atlanta, GA, USA.

Background And Aims: Oral drug delivery is the most attractive pathway for ulcerative colitis [UC] therapy, since it has many advantages. However, this strategy has encountered many challenges, including the instability of drugs in the gastrointestinal tract [GT], low targeting of disease tissues, and severe adverse effects. Nanoparticles capable of colitis tissue-targeted delivery and site-specific drug release may offer a unique and therapeutically effective system that addresses these formidable challenges. Read More

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January 2018

Stability and bioavailability of diltiazem/polyethylene oxide matrix tablets.

Pharm Dev Technol 2018 Dec 3;23(10):1057-1066. Epub 2017 Jul 3.

a Industrial Pharmacy Laboratory, Medical and Pharmaceutical Chemistry Department, Division of Pharmaceutical Industries , National Research Centre (Affiliation ID: 10014618) , Dokki, Giza , Egypt.

The aim of this study was to prepare and evaluate in vitro and in vivo; Diltiazem-Hydrochloride (DTZ) in sustained-release matrix tablets. Stability of DTZ tablets prepared with polyethylene oxide (MWs 900 000, 4 000 000, and 8 000 000) with or without addition of electrolytes was carried-out for 1-month, under short-term storage at 40 °C/75% RH. Stability was evaluated by DTZ content, DSC and drug release using the Flow-Through Cell (USP # IV). Read More

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December 2018

Screening and identification of the tumor-associated antigen CK10, a novel potential liver cancer marker.

FEBS Open Bio 2017 05 21;7(5):627-635. Epub 2017 Mar 21.

School of Life Sciences Zhengzhou University China.

Hepatocellular carcinoma (HCC) is a malignancy that is associated with high mortality rates in Asia. These tumors are highly invasive and their etiology is frequently unknown. Thus, most patients are diagnosed in the middle and late stages of the disease, and thus do not have sufficient time for therapy. Read More

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Inclusion Complexation of Etodolac with Hydroxypropyl-beta-cyclodextrin and Auxiliary Agents: Formulation Characterization and Molecular Modeling Studies.

Mol Pharm 2017 04 1;14(4):1231-1242. Epub 2017 Mar 1.

Department of Quality Assurance, SVKM's Dr. Bhanuben Nanavati College of Pharmacy , Gate No. 1, SVKM Campus, V. M. Road, Vile Parle (W), Mumbai 400 056, India.

The present investigation was aimed to prepare inclusion complexes of a therapeutically important nonsteroidal anti-inflammatory drug, etodolac (ETD) with hydroxypropyl-beta-cyclodextrin (HP-β-CD) and to study the effect of l-arginine (l-Arg) as an auxiliary agent on the complexation efficiency of HP-β-CD to improve aqueous solubility and the dissolution property of ETD. The binary and ternary complexes were prepared by physical mixing, coevaporation, and spray drying methods. The complexes were characterized using differential scanning colorimetry (DSC), Fourier transform-infrared spectroscopy (FT-IR), and powder X-ray diffraction (PXRD) studies. Read More

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LIN28A enhances the therapeutic potential of cultured neural stem cells in a Parkinson's disease model.

Brain 2016 Oct 18;139(Pt 10):2722-2739. Epub 2016 Aug 18.

1 Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea 2 Hanyang Biomedical Research Institute, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea 3 Graduate School of Biomedical Science and Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea

The original properties of tissue-specific stem cells, regardless of their tissue origins, are inevitably altered during in vitro culturing, lessening the clinical and research utility of stem cell cultures. Specifically, neural stem cells derived from the ventral midbrain lose their dopamine neurogenic potential, ventral midbrain-specific phenotypes, and repair capacity during in vitro cell expansion, all of which are critical concerns in using the cultured neural stem cells in therapeutic approaches for Parkinson's disease. In this study, we observed that the culture-dependent changes of neural stem cells derived from the ventral midbrain coincided with loss of RNA-binding protein LIN28A expression. Read More

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October 2016

Designing of a phytosome dosage form with Tecomella undulata as a novel drug delivery for better utilization.

Pak J Pharm Sci 2016 Jul;29(4):1231-5

Department of Pharmacognosy, Akal College of Pharmacy, Sangroor, India.

In recent past scientists are moving for the novel herbal medicines for treatment of almost all diseases as they have no or lesser adverse effects as compared with modern allopathic medicines. The potency of any dosage form is depending on effective drug delivery level of any therapeutically active drug molecule. Phytosome is a novel approach to drug delivery system that produce more absorption and utilization than conventional herbal extracts and shows enhanced bioavailability. Read More

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Voriconazole-Loaded Nanostructured Lipid Carriers for Ocular Drug Delivery.

Cornea 2016 Jun;35(6):866-71

*Laboratory of Pharmaceutical Technology, School of Pharmacy-Universidade Federal de Goiás (UFG), Goiânia, GO, Brasil; and †Laboratory of Food, Drugs and Cosmetics (LTMAC), Universidade de Brasília (UnB), Brasília, DF, Brasil.

Purpose: To design and evaluate the potential of a topical delivery system for ocular administration of voriconazole, based on cationic nanostructured lipid carriers (NLCs).

Methods: NLC dispersions composed of glyceryl behenate/capric caprylic triglyceride, polysorbate 80, sorbitan trioleate, and cetylpyridinium chloride were obtained and characterized. Ex vivo permeations experiments were performed to evaluate their drug delivery potential. Read More

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Conjugation of curcumin-loaded lipid nanoemulsions with cell-penetrating peptides increases their cellular uptake and enhances the anti-inflammatory effects in endothelial cells.

J Pharm Pharmacol 2016 Feb 8;68(2):195-207. Epub 2016 Jan 8.

Institute of Cellular Biology and Pathology "Nicolae Simionescu" of the Romanian Academy, Bucharest, Romania.

Objectives: To prepare and characterize in vitro and in vivo lipid nanoemulsions (LN) loaded with curcumin (Cm) and functionalized with a cell-penetrating peptide.

Methods: Curcumin-loaded lipid nanoemulsions (CmLN) functionalized with a nona-arginine peptide (R9-CmLN) have been obtained, characterized and optimized for size, entrapment efficiency and in vitro Cm release. The interaction of R9-CmLN with human endothelial cells (HEC) was investigated using cultured EA. Read More

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February 2016

Development and characterization of pH responsive polymeric nanoparticles of SN-38 for colon cancer.

Artif Cells Nanomed Biotechnol 2016 Dec 5;44(8):1824-1834. Epub 2015 Nov 5.

a Institute of Pharmaceutical Sciences, Guru Ghasidas Vishwavidyalaya , Bilaspur , Chhattisgarh , India.

7-Ethyl-10-hydroxycamptothecin (SN-38) is 1000 times more cytotoxic than its prodrug Irinotecan hydrochloride (CPT-11). It is not used therapeutically because of its insolubility in acceptable solvents. The objective of the present study was to prepare chitosan nanoparticles (CsENP) of SN-38 by polyelectrolyte complexation method using the Box-Behnken design. Read More

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December 2016

A tumor lysate is an effective vaccine antigen for the stimulation of CD4(+) T-cell function and subsequent induction of antitumor immunity mediated by CD8(+) T cells.

Cancer Biol Ther 2015 21;16(11):1616-25. Epub 2015 Sep 21.

b Department of Life and Environmental Sciences ; Chiba Institute of Technology ; Narashino , Chiba , Japan.

To develop a potent cancer vaccine, it is important to study how to prepare highly immunogenic antigens and to identify the most appropriate adjuvants for the antigens. Here we show that a tumor lysate works as an effective antigen to prime CD4(+) T-cell help when baculovirus is employed as an adjuvant. When immunized intradermally with the combination (BLP) of baculovirus, a CT26 tumor lysate, and a cytotoxic T-cell epitope peptide before a tumor challenge, 60% of mice rejected tumors. Read More

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September 2016

Cancer immunology for the clinician.

Authors:
Louis M Weiner

Clin Adv Hematol Oncol 2015 May;13(5):299-306

Georgetown Lombardi Comprehensive Cancer Center, Washington, DC.

Cancer immunotherapy is coming of age. It has become abundantly clear that immunotherapy-which has been described as treating the body's immune system so the immune system can treat the cancer-can be routinely effective, and may indeed cure advanced cancers. Accordingly, it is important to understand the basic, clinically relevant principles of cancer immunology to better prepare for an increasingly exciting future. Read More

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Adaptability and potential for treatment of placental functions to improve embryonic development and postnatal health.

Authors:
James C Cross

Reprod Fertil Dev 2016 ;28(1-2):75-82

Departments of Comparative Biology and Experimental Medicine, Biochemistry and Molecular Biology, Medical Genetics, and Obstetrics and Gynecology, University of Calgary, Calgary, Alberta T2N 4N1, Canada. Email:

For an organ that is so critical for life in eutherian mammals, the placenta hardly gets the attention that it deserves. The placenta does a series of remarkable things, including implanting the embryo in the uterus, negotiating with the mother for nutrients but also protecting her health during pregnancy, helping establish normal metabolic and cardiovascular function for life postnatally (developmental programming) and initiating changes that prepare the mother to care for and suckle her young after birth. Different lines of evidence in experimental animals suggest that the development and function of the placenta are adaptable. Read More

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January 2017