Methods Enzymol 2021 7;654:139-167. Epub 2021 Apr 7.
Department of Molecular Pharmacology and Therapeutics, Columbia University, Vagelos College of Physicians and Surgeons, New York, NY, United States; Department of Physiology and Cellular Biophysics, Columbia University, Vagelos College of Physicians and Surgeons, New York, NY, United States. Electronic address:
Plasma membrane-localized ion channels are essential for diverse physiological processes such as neurotransmission, muscle contraction, and osmotic homeostasis. The surface density of such ion channels is a major determinant of their function, and tuning this variable is a powerful way to regulate physiology. Dysregulation of ion channel surface density due to inherited or de novo mutations underlies many serious diseases, and molecules that can correct trafficking deficits are potential therapeutics and useful research tools. Read More