3,685 results match your criteria potato virus

Molecular insights on potato yellow vein crinivirus infections in the highlands of Colombia.

J Gen Virol 2021 Jun;102(6)

Faculty of Basic and Applied Sciences, Universidad Militar Nueva Granada, Km 2 via Cajicá-Zipaquirá, Cajicá, Cundinamarca, Colombia.

(PYVV) was detected in potatoes grown in the Central highlands, north of Bogotá (~3000 m altitude), Colombia. At this altitude viral whitefly vectors are largely absent, but infection persists because of the use of uncertified tubers. Plants with typical PYVV-induced yellowing symptoms, as well as with atypical yellowing or non-symptomatic symptoms were sampled at three separate geographical locations. Read More

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Extreme resistance to potato virus A in potato cultivar Barbara is independently mediated by Ra and Rysto.

Plant Dis 2021 Jun 7. Epub 2021 Jun 7.

agriculture and Agri-Food Canada, 850 Lincoln Road, P.O. Box 20280, Fredericton, New Brunswick, Canada, E3B0E2;

Potato virus A (PVA) and potato virus Y (PVY) are two common members of Potyvirus genus infecting potato crops worldwide. Host resistance offers an economical and effective means for the control and/or management of these viruses. In this study, 20 potato clones were screened for their resistance against PVA and PVY by mechanical and/or graft inoculation assay, and were explored for the relationship between extreme resistance genes Ra and Ry by the detection of molecular markers linked respectively to Ryadg, Rysto, and Rychc. Read More

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J Nutr Biochem 2021 Jun 2:108787. Epub 2021 Jun 2.

Núcleo Integrado de Desenvolvimento em Análises Laboratoriais (NIDAL), Departamento de Tecnologia e Ciência dos Alimentos, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.

The outbreak of mysterious pneumonia at the end of 2019 is associated with widespread research interest worldwide. The coronavirus disease-19 (COVID-19) targets multiple organs through inflammatory, immune, and redox mechanisms, and no effective drug for its prophylaxis or treatment has been identified until now. The use of dietary bioactive compounds, such as phenolic compounds (PC), has emerged as a putative nutritional or therapeutic adjunct approach for COVID-19. Read More

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biological strain group YD: hypersensitive resistance genes elicited and phylogenetic placement.

Plant Dis 2021 Jun 3. Epub 2021 Jun 3.

Fera Science Ltd, 16233, York, Yorkshire, United Kingdom of Great Britain and Northern Ireland;

Potato virus Y (PVY) disrupts healthy seed potato production and causes tuber yield and quality losses globally. Its subdivisions consist of strain groups defined by potato hypersensitive resistance (HR) genes and phylogroups defined by sequencing. When PVY isolate PP was inoculated to potato cultivar differentials with HR genes, the HR phenotype pattern obtained resembled that caused by strain group PVY isolate KIP1. Read More

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High Throughput Sequencing-Aided Survey Reveals Widespread Mixed Infections of Whitefly-Transmitted Viruses in Cucurbits in Georgia, USA.

Viruses 2021 May 26;13(6). Epub 2021 May 26.

Department of Plant Pathology, University of Georgia, Tifton, GA 31793, USA.

Viruses transmitted by the sweet potato whitefly () have been detrimental to the sustainable production of cucurbits in the southeastern USA. Surveys were conducted in the fall of 2019 and 2020 in Georgia, a major cucurbit-producing state of the USA, to identify the viruses infecting cucurbits and their distribution. Symptomatic samples were collected and small RNA libraries were prepared and sequenced from three cantaloupes, four cucumbers, and two yellow squash samples. Read More

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Revealing the Complexity of Sweepovirus-Deltasatellite-Plant Host Interactions: Expanded Natural and Experimental Helper Virus Range and Effect Dependence on Virus-Host Combination.

Microorganisms 2021 May 10;9(5). Epub 2021 May 10.

Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Consejo Superior de Investigaciones Científicas-Universidad de Málaga (IHSM-CSIC-UMA), 29750 Algarrobo-Costa, Málaga, Spain.

Sweepoviruses are begomoviruses (genus , family ) with ssDNA genomes infecting sweet potato and other species of the family Convolvulaceae. Deltasatellites (genus , family ) are small-size non-coding DNA satellites associated with begomoviruses. In this study, the genetic diversity of deltasatellites associated with sweepoviruses infecting plants was analyzed by further sampling the populations where the deltasatellite sweet potato leaf curl deltasatellite 1 (SPLCD1) was initially found, expanding the search to other geographical areas in southern continental Spain and the Canary Islands. Read More

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A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants.

Viruses 2021 05 11;13(5). Epub 2021 May 11.

John Innes Centre, Department of Biochemistry and Metabolism, Norwich Research Park, Norwich NR4 7UH, UK.

The production of plant helical virus-like particles (VLPs) via plant-based expression has been problematic with previous studies suggesting that an RNA scaffold may be necessary for their efficient production. To examine this, we compared the accumulation of VLPs from two potexviruses, papaya mosaic virus and alternanthera mosaic virus (AltMV), when the coat proteins were expressed from a replicating potato virus X- based vector (pEff) and a non-replicating vector (pEAQ-). Significantly greater quantities of VLPs could be purified when pEff was used. Read More

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The Resistance Responses of Potato Plants to Potato Virus Y Are Associated with an Increased Cellular Methionine Content and an Altered SAM:SAH Methylation Index.

Viruses 2021 May 21;13(6). Epub 2021 May 21.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia.

Plant-virus interactions are frequently influenced by elevated temperature, which often increases susceptibility to a virus, a scenario described for potato cultivar Chicago infected with potato virus Y (PVY). In contrast, other potato cultivars such as Gala may have similar resistances to PVY at both normal (22 °C) and high (28 °C) temperatures. To elucidate the mechanisms of temperature-independent antivirus resistance in potato, we analysed responses of Gala plants to PVY at different temperatures using proteomic, transcriptional and metabolic approaches. Read More

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Rapid and sensitive detection of potato virus X by one-step reverse transcription-recombinase polymerase amplification method in potato leaves and dormant tubers.

Mol Cell Probes 2021 May 27;58:101743. Epub 2021 May 27.

ICAR-Central Potato Research Institute, Regional Station, Modipuram, 250110, Uttar Pradesh, India. Electronic address:

Potato virus X (PVX), is a serious threat to global potato production. A simple and rapid detection method is imperative for PVX diagnosis and early management. In this study, an isothermal one-step reverse transcription-recombinase polymerase amplification (RT-RPA) method was optimized for the quick and convenient detection of PVX in potato leaves and tubers. Read More

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First Report of 'Candidatus Phytoplasma trifolii' Related Strain Associated with Yellowing and Witches'-Broom of Industrial Hemp (Cannabis sativa) in Arizona.

Jiahuai Hu

Plant Dis 2021 May 25. Epub 2021 May 25.

University of Arizona, School of Plant Sciences, 1140 E. South Campus Dr., Forbes Building, Room 303, Tucson, Arizona, United States, 85721;

In Arizona, industrial hemp (Cannabis sativa) is a newly cultivated crop for fiber, oil, cosmetic products, and health food. During July to September 2020, two fields of industrial hemp crops were identified in southern Arizona with 10 to 30% incidence of plants showing witches' broom. Disease incidence was assessed by counting symptomatic plants in 4 randomly selected rows of 25 plants in each field. Read More

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Affinity Purification-Mass Spectrometry Identifies a Novel Interaction between a Polerovirus and a Conserved Innate Immunity Aphid Protein that Regulates Transmission Efficiency.

J Proteome Res 2021 06 21;20(6):3365-3387. Epub 2021 May 21.

Emerging Pests and Pathogens Research Unit, USDA Agricultural Research Service, Ithaca, New York 14853, United States.

The vast majority of plant viruses are transmitted by insect vectors, with many crucial aspects of the transmission process being mediated by key protein-protein interactions. Still, very few vector proteins interacting with viruses have been identified and functionally characterized. (PLRV) is transmitted most efficiently by , the green peach aphid, in a circulative, non-propagative manner. Read More

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Purine Nucleoside Derivatives Containing a Sulfa Ethylamine Moiety: Design, Synthesis, Antiviral Activity, and Mechanism.

J Agric Food Chem 2021 May 14;69(20):5575-5582. Epub 2021 May 14.

State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, P. R. China.

To find efficient and broad-spectrum viral agents, a series of purine nucleoside derivatives containing sulfa ethylamine moieties was designed and synthesized, and their antiviral activities against tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), and potato virus Y (PVY) were evaluated. Some target compounds displayed good antiviral activities. Among them, compound showed excellent protective activity against CMV and PVY with 50% effective concentration values (EC) of 137 and 209 μg/mL, respectively, which were better than that of the control agent ningnanmycin (508 and 431 μg/mL). Read More

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Molecular and biological characterization of Potato virus Y detected in zucchini in China.

Virusdisease 2021 Mar 18;32(1):117-122. Epub 2021 Mar 18.

College of Hortculture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, 010019 Inner Mongolia China.

Potato virus Y (PVY) is an important viral pathogen of the plant family . However, it has not been reported that PVY infects zucchini ( L). Zucchini plants showing symptoms of leaf mosaic and fruit ringspot were collected from Inner Mongolia, China. Read More

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Effect of potato apical leaf curl disease on glycemic index and resistant starch of potato (Solanum tuberosum L.) tubers.

Food Chem 2021 Oct 23;359:129939. Epub 2021 Apr 23.

ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh, India.

Tomato leaf curl New Delhi virus-potato (ToLCNDV-potato) causes potato apical leaf curl disease which severely affects nutritional parameters such as carbohydrate, protein, and starch biosynthesis thereby altering glycemic index (GI) and resistant starch (RS) of potato. ToLCNDV-potato virus was inoculated on potato cultivars (Kufri Pukhraj [susceptible]; Kufri Bahar [resistant]) and various quality parameters of potato tuber were studied. There was a significant (P < 0. Read More

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October 2021

Bioengineering potato plants to produce benzylglucosinolate for improved broad-spectrum pest and disease resistance.

Transgenic Res 2021 May 6. Epub 2021 May 6.

Department of Plant and Environmental Sciences, DynaMo Center, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark.

In traditional, small-scale agriculture in the Andes, potatoes are frequently co-cultivated with the Andean edible tuber Tropaeolum tuberosum, commonly known as mashua, which is believed to exert a pest and disease protective role due to its content of the phenylalanine-derived benzylglucosinolate (BGLS). We bioengineered the production of BGLS in potato by consecutive generation of stable transgenic events with two polycistronic constructs encoding for expression of six BGLS biosynthetic genes from Arabidopsis thaliana. First, we integrated a polycistronic construct coding for the last three genes of the pathway (SUR1, UGT74B1 and SOT16) into potato driven by the cauliflower mosaic virus 35S promoter. Read More

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P1 of shows strong adaptation capacity, replacing P1-HCPro in a chimeric .

J Virol 2021 May 5. Epub 2021 May 5.

Centro Nacional de Biotecnología CNB, CSIC, Madrid, Spain

is the largest family of plant RNA viruses. Their genomes are expressed through long polyproteins that are usually headed by the leader endopeptidase P1. This protein can be classified as Type A or Type B based on host proteolytic requirements and RNA silencing suppression (RSS) capacity. Read More

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First report of Tomato black ring virus infecting raspberry and blackberry in Poland.

Plant Dis 2021 May 2. Epub 2021 May 2.

Warsaw University of Life Sciences, 49561, Plant Protection, Section of Phytopathology, Warsaw, Poland;

Raspberry ( L.) and blackberry ( L.) are infected by at least 29 viruses, including the Tomato black ring virus (TBRV) (Martin et al. Read More

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Molecular characterization and infectivity analysis of tomato leaf curl New Delhi virus isolates infecting potato.

3 Biotech 2021 Apr 30;11(4):203. Epub 2021 Mar 30.

ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh 171 001 India.

Nucleotide sequence of complete genome of a new isolate (KAN-6) of tomato leaf curl New Delhi virus (ToLCNDV) from Kanpur, Uttar Pradesh, India was determined. Sequence analysis indicated that it shared maximum identity to ToLCNDV isolates from pumpkin and ashgourd. Infectious clones of isolate KAN-6 along with two other ToLCNDV isolates (MOD-21 & FAI-19) obtained from potato fields of Modipuram and Faizabad, India were produced and used in symptom expression studies in and potato plants through agro-inoculation. Read More

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Argonaute 2 Controls Antiviral Activity against Sweet Potato Mild Mottle Virus in .

Plants (Basel) 2021 Apr 26;10(5). Epub 2021 Apr 26.

Biological Research Center Szeged, Institute of Plant Biology, Photo- and Chronobiology Group Eötvös Loránd Research Network (ELKH), H-6726 Szeged, Hungary.

RNA silencing is a sequence specific post-transcriptional mechanism regulating important biological processes including antiviral defense in plants. Argonaute (AGO) proteins, the catalytic subunits of the silencing complexes, are loaded with small RNAs to execute the sequence specific RNA cleavage or translational inhibition. Plants encode several AGO proteins and a few of them, especially AGO1 and AGO2, have been shown to be required for antiviral silencing. Read More

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Molecular Characterization of Potato Virus Y (PVY) Using High-Throughput Sequencing: Constraints on Full Genome Reconstructions Imposed by Mixed Infection Involving Recombinant PVY Strains.

Plants (Basel) 2021 Apr 12;10(4). Epub 2021 Apr 12.

INRAE, University of Bordeaux, UMR BFP, 33140 Villenave d'Ornon, France.

In recent years, high throughput sequencing (HTS) has brought new possibilities to the study of the diversity and complexity of plant viromes. Mixed infection of a single plant with several viruses is frequently observed in such studies. We analyzed the virome of 10 tomato and sweet pepper samples from Slovakia, all showing the presence of potato virus Y (PVY) infection. Read More

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A Primer on the Analysis of High-Throughput Sequencing Data for Detection of Plant Viruses.

Microorganisms 2021 Apr 14;9(4). Epub 2021 Apr 14.

Plant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food, Burg. Van Gansberghelaan 96, 9820 Merelbeke, Belgium.

High-throughput sequencing (HTS) technologies have become indispensable tools assisting plant virus diagnostics and research thanks to their ability to detect any plant virus in a sample without prior knowledge. As HTS technologies are heavily relying on bioinformatics analysis of the huge amount of generated sequences, it is of utmost importance that researchers can rely on efficient and reliable bioinformatic tools and can understand the principles, advantages, and disadvantages of the tools used. Here, we present a critical overview of the steps involved in HTS as employed for plant virus detection and virome characterization. Read More

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The Phylogeography of Potato Virus X Shows the Fingerprints of Its Human Vector.

Viruses 2021 04 9;13(4). Epub 2021 Apr 9.

UWA Institute of Agriculture, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

Potato virus X (PVX) occurs worldwide and causes an important potato disease. Complete PVX genomes were obtained from 326 new isolates from Peru, which is within the potato crop's main domestication center, 10 from historical PVX isolates from the Andes (Bolivia, Peru) or Europe (UK), and three from Africa (Burundi). Concatenated open reading frames (ORFs) from these genomes plus 49 published genomic sequences were analyzed. Read More

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Degradome Analysis of Tomato and Plants Infected with Potato Spindle Tuber Viroid.

Int J Mol Sci 2021 Apr 2;22(7). Epub 2021 Apr 2.

Istituto de Biología Molecular y Celular de Plantas (CSIC-UPV), 46022 Valencia, Spain.

Viroids are infectious non-coding RNAs that infect plants. During infection, viroid RNAs are targeted by Dicer-like proteins, generating viroid-derived small RNAs (vd-sRNAs) that can guide the sequence specific cleavage of cognate host mRNAs via an RNA silencing mechanism. To assess the involvement of these pathways in pathogenesis associated with nuclear-replicating viroids, high-throughput sequencing of sRNAs and degradome analysis were carried out on tomato and plants infected by potato spindle tuber viroid (PSTVd). Read More

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Complete genome sequences of novel Berlinvirus and novel Certrevirus lytic for Pectobacterium sp. causing soft rot and black leg disease of potato.

Virus Genes 2021 Jun 29;57(3):302-305. Epub 2021 Apr 29.

University of Chemistry and Technology Prague, Technická 1905/5, Prague 6-Dejvice, Prague, Czech Republic.

Two novel dsDNA bacteriophages named Pectobacterium virus CB251 (PcCB251) and Pectobacterium virus CB7V (PcCB7V) targeting plant pathogen Pectobacterium parmentieri have been isolated and sequenced. The PcCB251 genome consists of 40,557 bp with G+C content of 48.6% and contains 47 predicted genes on a single strand. Read More

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Complete genome sequence of a divergent sweet potato chlorotic stunt virus isolate infecting Calystegia hederacea in China.

Arch Virol 2021 Jul 26;166(7):2037-2040. Epub 2021 Apr 26.

Institute of Plant Protection, Henan Academy of Agricultural Sciences, Postgraduate T&R Base of Zhengzhou University, Zhengzhou, 450002, China.

Sweet potato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae) is one of the most destructive viruses infecting sweet potatoes. In this study, we determined the complete genome sequence of an SPCSV-like isolate (CH) from Calystegia hederacea Wall. (Convolvulaceae), a weed species related to sweet potato, by combining next-generation sequencing and rapid amplification of cDNA ends. Read More

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Extreme Resistance to Viruses in Potato and Soybean.

Front Plant Sci 2021 6;12:658981. Epub 2021 Apr 6.

Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT, United States.

Plant pathogens, including viruses, negatively impact global crop production. Plants have evolved complex immune responses to pathogens. These responses are often controlled by nucleotide-binding leucine-rich repeat proteins (NLRs), which recognize intracellular, pathogen-derived proteins. Read More

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Genetic parameters, prediction, and selection in a white Guinea yam early-generation breeding population using pedigree information.

Crop Sci 2021 Mar-Apr;61(2):1038-1051. Epub 2020 Dec 22.

International Institute of Tropical Agriculture (IITA) Ibadan Nigeria.

Better understanding of the genetic control of traits in breeding populations is crucial for the selection of superior varieties and parents. This study aimed to assess genetic parameters and breeding values for six essential traits in a white Guinea yam ( Poir.) breeding population. Read More

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December 2020

Cucumber Mosaic Virus 1a Protein Interacts with the Tobacco SHE1 Transcription Factor and Partitions between the Nucleus and the Tonoplast Membrane.

Plant Pathol J 2021 Apr 1;37(2):182-193. Epub 2021 Apr 1.

Department of Horticulture Sciences, Seoul Women's University, Seoul 01797, Korea.

The transcription factor SHE1 was identified as an interacting partner with the cucumber mosaic virus (CMV) 1a protein in the yeast two-hybrid system, by a pull-down assay, and via bimolecular fluorescent complementation. Using fluorescent-tagged proteins and confocal microscopy, the CMV 1a protein itself was found distributed predominantly between the nucleus and the tonoplast membrane, although it was also found in speckles in the cytoplasm. The SHE1 protein was localized in the nucleus, but in the presence of the CMV 1a protein was partitioned between the nucleus and the tonoplast membrane. Read More

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Universal primers for rapid detection of six pospiviroids in Solanaceae plants using one-step RT-PCR and RT-LAMP.

Plant Dis 2021 Apr 14. Epub 2021 Apr 14.

National Chung Hsing University, Department of Plant Pathology, 250 Kuo-Kuang Rd, Taichung, Taiwan, 402;

A number of viruses and viroids infect solanaceous plants causing severe yield losses. Several seed-borne viroids are currently listed as quarantine pathogens in many countries. Among them, columnea latent viroid (CLVd), pepper chat fruit viroid (PCFVd), potato spindle tuber viroid (PSTVd), tomato apical stunt viroid (TASVd), tomato chlorotic dwarf viroid (TCDVd), and tomato planta macho viroid (TPMVd) are of major concerns. Read More

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The Structure of the Potato Virus A Particles Elucidated by Small Angle X-Ray Scattering and Complementary Techniques.

Biochemistry (Mosc) 2021 Feb;86(2):230-240

Shubnikov Institute of Crystallography of Federal Scientific Research Centre "Crystallography and Photonics" of the Russian Academy of Sciences, Moscow, 119333, Russia.

Potato virus A (PVA) protein coat contains on its surface partially unstructured N-terminal domain of the viral coat protein (CP), whose structural and functional characteristics are important for understanding the mechanism of plant infection with this virus. In this work, we investigated the properties and the structure of intact PVA and partially trypsinized PVAΔ32 virions using small-angle X-ray scattering (SAXS) and complimentary methods. It was shown that after the removal of 32 N-terminal amino acids of the CP, the virion did not disintegrate and remained compact, but the helical pitch of the CP packing changed. Read More

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February 2021