23 results match your criteria position pvy

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The Structure of the Potato Virus A Particles Elucidated by Small Angle X-Ray Scattering and Complementary Techniques.

Biochemistry (Mosc) 2021 Feb;86(2):230-240

Shubnikov Institute of Crystallography of Federal Scientific Research Centre "Crystallography and Photonics" of the Russian Academy of Sciences, Moscow, 119333, Russia.

Potato virus A (PVA) protein coat contains on its surface partially unstructured N-terminal domain of the viral coat protein (CP), whose structural and functional characteristics are important for understanding the mechanism of plant infection with this virus. In this work, we investigated the properties and the structure of intact PVA and partially trypsinized PVAΔ32 virions using small-angle X-ray scattering (SAXS) and complimentary methods. It was shown that after the removal of 32 N-terminal amino acids of the CP, the virion did not disintegrate and remained compact, but the helical pitch of the CP packing changed. Read More

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February 2021

Small RNA profiling analysis of two recombinant strains of potato virus Y in infected tobacco plants.

Virus Res 2020 10 22;288:198125. Epub 2020 Aug 22.

Department of Plant Pathology and Ecology, Connecticut Agricultural Experiment Station, New Haven, CT 06511, United States. Electronic address:

Plant viral infections lead to accumulation of virus-derived small interfering RNAs (vsiRNAs) as a result of host defense mechanisms. High-throughput sequencing technology enables vsiRNA profiling analyses from virus infected plants, which provide important insights into virus-host interactions. Potato virus Y (PVY) is a detrimental plant pathogen that can infect a variety of solanaceous crops, e. Read More

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October 2020

The amino acid 419 in HC-Pro is involved in the ability of PVY isolate N605 to induce necrotic symptoms on potato tubers.

Virus Res 2015 Oct 10;208:110-9. Epub 2015 Jun 10.

INRA-Cirad-Supagro Montpellier, UMR 385 BGPI, F-34398 Montpellier, France. Electronic address:

The ability to induce the potato tuber necrosis ringspot disease (PTNRD) is a property shared by PVY isolates belonging to different groups (e.g. PVY(N) and PVY(O)) and variants (e. Read More

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October 2015

Evolutionary Pathways to Break Down the Resistance of Allelic Versions of the PVY Resistance Gene va.

Plant Dis 2014 Nov;98(11):1521-1529

INRA-Agrocampus Ouest-Université Rennes 1 and INRA-Cirad Montpellier SupAgro.

Emergence of viral genotypes can make control strategies based on resistance genes ineffective. A few years after the deployment of tobacco genotypes carrying alleles of the Potato virus Y (PVY) recessive resistance gene va, virulent PVY isolates have been reported, suggesting the low durability of va. To have a broader view of the evolutionary processes involved in PVY adaptation to va, we studied mutational pathways leading to the emergence of PVY resistance-breaking populations. Read More

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November 2014

Full-genome analyses of a Potato Virus Y (PVY) isolate infecting pepper (Capsicum annuum L.) in the Republic of South Africa.

Virus Genes 2014 Dec 11;49(3):466-76. Epub 2014 Oct 11.

Discipline of Plant Pathology, School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, Private Bag X01, Scottsville, Pietermaritzburg, 3209, South Africa.

Potato Virus Y (PVY) is a pathogen of economic importance in pepper and other major crop species in the family Solanaceae. Three major PVY strain groups: O, C, and N, have been distinguished on the basis of genome sequencing. In this study, the first full-genome sequence of a PVY isolate (JVW-186) infecting pepper from the province of KwaZulu-Natal, Republic of South Africa is reported. Read More

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December 2014

Hypersensitive response to in potato cultivar Sárpo Mira is conferred by the - gene located on the long arm of chromosome IX.

Mol Breed 2014 19;34:471-480. Epub 2014 Feb 19.

Plant Breeding and Acclimatization Institute-National Research Institute, Młochów Research Centre, Platanowa 19, 05-831 Młochów, Poland.

(PVY, ) is the fifth most important plant virus worldwide in terms of economic and scientific impact. It infects members of the family Solanaceae and causes losses in potato, tomato, tobacco, pepper and petunia production. In potato and its wild relatives, two types of resistance genes against PVY have been identified. Read More

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February 2014

Sequence variation of P1 gene in Potato virus Y isolated from Fujian Province.

Yi Chuan 2014 Jul;36(7):713-22

Key Laboratory of Plant Virology of Fujian Province, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

To understand the sequence variation and the putative protein structure of P1 gene in Potato virus Y (PVY) and to identify the sources of the variation, P1 gene in PVY isolated from Fujian Province was amplified by reverse-transcription polymerase chain reaction (RT-PCR) using a pair of degenerate primers designed from the conserved regions of published sequences. Sequence variation and putative protein structure were analyzed, and phylogenetic tree was reconstructed using Bayesian inference method. Expected fragments of 915 bp in size were amplified from 12 samples collected from Fujian Province by RT-PCR. Read More

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Comparative analysis of virus-specific small RNA profiles of three biologically distinct strains of Potato virus Y in infected potato (Solanum tuberosum) cv. Russet Burbank.

Virus Res 2014 Oct 15;191:153-60. Epub 2014 Jul 15.

Department of Plant Pathology, Washington State University, Pullman, WA, USA. Electronic address:

Deep sequencing technology has enabled the analysis of small RNA profiles of virus-infected plants and could provide insights into virus-host interactions. Potato virus Y is an economically important viral pathogen of potato worldwide. In this study, we investigated the nature and relative levels of virus-derived small interfering RNAs (vsiRNAs) in potato cv. Read More

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October 2014

First Report of Recombinant Potato virus Y Strains Infecting Potato in Jordan.

Plant Dis 2014 Jul;98(7):1017

Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.

Potato (Solanum tuberosum L.) is an important vegetable crop in Jordan, occupying second position after olives. In 2012, potatoes were planted on about 6,000 ha with a production of about 141,000 t (2). Read More

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Molecular and Serological Typing of Potato virus Y Isolates from Brazil Reveals a Diverse Set of Recombinant Strains.

Plant Dis 2012 Oct;96(10):1451-1458

Department of PSES, University of Idaho.

In Brazil, Potato virus Y (PVY) currently presents a significant problem for potato production, reducing tuber yield and quality. Recombinant tuber necrotic isolates of PVY had been reported to occur in the country but no systematic study of the PVY isolate diversity was conducted thus far. Here, a panel of 36 PVY isolates, randomly collected in Brazil from potato between 1985 and 2009, was subjected to a systematic molecular and serological typing using reverse-transcription polymerase chain reaction and a series of PVY- and PVY-specific monoclonal antibodies. Read More

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October 2012

dN/dS-based methods detect positive selection linked to trade-offs between different fitness traits in the coat protein of potato virus Y.

Mol Biol Evol 2011 Sep 15;28(9):2707-17. Epub 2011 Apr 15.

UR407 Pathologie Végétale, Institut National de la Recherche Agronomique, Montfavet, France.

The dN/dS ratio between nonsynonymous and synonymous substitution rates has been used extensively to identify codon positions involved in adaptive processes. However, the accuracy of this approach has been questioned, and very few studies have attempted to validate experimentally its predictions. Using the coat protein (CP) of Potato virus Y (PVY; genus Potyvirus, family Potyviridae) as a case study, we identified several candidate positively selected codon positions that differed between clades. Read More

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September 2011

A point mutation in the polymerase of Potato virus Y confers virulence toward the Pvr4 resistance of pepper and a high competitiveness cost in susceptible cultivar.

Mol Plant Microbe Interact 2010 Jun;23(6):823-30

INRA, UR407 Pathologie Végétale, R-84140 Montfavet, France.

To understand why the Pvr4 resistance of pepper against Potyvirus spp. remained durable in field conditions while virulent Potato virus Y (PVY) variants could be selected in the laboratory, we studied the molecular mechanisms which generated these variants and the consequences on viral fitness. Using a reverse genetics approach with an infectious cDNA clone of PVY, we found that the region coding for the NIb protein (RNA-dependent RNA polymerase) of PVY was the avirulence factor corresponding to Pvr4 and that a single nonsynonymous nucleotide substitution in that region, an adenosine to guanosine substitution at position 8,424 of the PVY genome (A(8424)G), was sufficient for virulence. Read More

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A point mutation changes the serotype of a potato virus Y isolate; genomic determination of the serotype of PVY strains.

Virus Genes 2007 Oct 13;35(2):359-67. Epub 2007 Jun 13.

Department of International Agricultural Development, Graduate School of Agriculture, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.

A Syrian isolate of Potato virus Y (PVY), named PVY-12, reacted to two monoclonal antibodies that are specific to PVY(O,C) and PVY(N) strains, although its coat protein (CP) belongs to the PVY(N) strain. Analysis of the CP of PVY-12 revealed that a point mutation in its N terminus switched it from PVY(N)-like to PVY(O)-like at this position. This mutation changed the second nucleotide of the codon that encodes the 29th amino acid of the CP of PVY-12 from A to G, which resulted in one amino acid substitution from Glu(29 )to Gly(29). Read More

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October 2007

Potato virus yisolated from pepper fields in Tehran Province.

Commun Agric Appl Biol Sci 2006 ;71(3 Pt B):1335-40

Collage of Horticultural Science and Plant Protection, University of Tehran, Karaj, Iran.

Potato Virus Y was known as the main cause of yellowing and vein necrosis of pepper in Tehran Province, using Double Antibody Sandwich Elisa (DAS-ELISA). Biological properties including host range of the isolate was determined after biological purification. Host range studies showed that pepper isolate of PVY caused vein clearing and mosaic symptoms on Datura metel and Capsicum annum, mosaic on Nicotiana tabacum cv. Read More

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September 2007

Characterisation of Potato virus Y nnp strain inducing veinal necrosis in pepper: a naturally occurring recombinant strain of PVY.

Arch Virol 2005 Apr 10;150(4):709-20. Epub 2004 Dec 10.

Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Università degli Studi della Basilicata, Potenza, Italy.

The full-length genome of Potato virus Y (PVY) nnp strain, recovered from pepper showing veinal necrosis of leaves, was cloned and sequenced, finding an organisation typical for PVY species. It consists of 9699 nucleotides (nt) excluding the 3' terminal poly(A) tail and contains an open reading frame of 9186 nt, encoding the putative polyprotein of 3061 amino acids. In ELISA, the isolate reacted with a monoclonal antibody specific for PVY(C) but not with antibodies against PVY(N) or PVY(O). Read More

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The complete nucleotide sequence of Pepper mottle virus-Florida RNA.

Arch Virol 2003 Jan;148(1):189-97

Department of Entomology and Plant Pathology, Auburn University, Auburn, Alabama 36849, USA.

The Pepper mottle virus-Florida (PepMoV-FL) RNA genome was cloned and sequenced, and shown to consist of 9,717 nucleotides (nt) excluding the poly (A) tail. A single open reading frame was identified beginning at nucleotide position 169 encoding a polyprotein of 3068 amino acids. Phylogenetic sequence analysis revealed that of 44 full-length viral RNA genomes analyzed within the family Potyviridae, PepMoV-FL was most closely related to PepMoV-California (PepMoV-CA), Potato virus Y-H (PVY-H), PVY-N, PVY(o) and Potato virus V-DV42 (PVV-DV42). Read More

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January 2003

First Report of Potato Tuber Necrotic Ringspot Disease Caused by PVY in Portugal.

Plant Dis 1997 Jun;81(6):694

Biologishe Bundesanstalt fur Land-und Fortwirtschaft, Institut fur Biochemie und Pflanzenvirologie, Braunschweig, Germany.

During the last 2 years, potato (Solanum tuberosum L.) tubers displaying superficial necrotic arcs and rings were found in central Portugal. These symptoms increased during storage, and diminished tuber quality of ware (fresh-market) potatoes; however, no internal necrosis, which is typical for infections caused by tobacco rattle virus or potato mop top virus, was observed. Read More

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Switches in the mode of transmission select for or against a poorly aphid-transmissible strain of potato virus Y with reduced helper component and virus accumulation.

J Gen Virol 1996 Jul;77 ( Pt 7):1343-7

Laboratoire de biologie Cellulaire, INRA, 78026 Versailles, France.

A poorly aphid-transmissible potato virus Y (PVY-PAT) variant emerged after several cycles of mechanical transmission of an initially aphid-transmissible (AT) isolate. Sequence analysis of the N-terminal region of the helper component-proteinase (HC-Pro) gene revealed a Lys to Glu change at a position previously found to abolish the HC-Pro aphid transmission activity in several potyviruses. Two cycles of aphid transmission allowed the virus population to evolve towards an AT form (PVY-ATnew) where a Glu to Lys change was observed. Read More

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The 5' untranslated region of PVY RNA, even located in an internal position, enables initiation of translation.

Virus Genes 1993 Dec;7(4):367-79

Station de Phytopathologie, Institut National de la Recherche Agronomique, Versailles, France.

Potato virus Y (PVY) is the type member of the potyvirus group. Potyviruses, like picorna-, como-, and nepoviruses, belong to the picornavirus-like supergroup. All these viral RNAs have a VPg at their 5' end, and for four picornaviruses and one comovirus internal initiation of translation has been reported. Read More

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December 1993

The complete nucleotide sequence of pepper mottle virus genomic RNA: comparison of the encoded polyprotein with those of other sequenced potyviruses.

Virology 1992 Nov;191(1):19-30

Department of Biological Sciences, University of South Carolina, Columbia 29208.

The complete nucleotide sequence of a pepper mottle virus isolate from California (PepMoV C) has been determined from cloned viral cDNAs. The PepMoV C genomic RNA is 9640 nucleotides excluding the poly(A) tail and contains a long open reading frame starting at nucleotide 168 and potentially encoding a polyprotein of 3068 amino acids. Comparison of the PepMoV C presumptive polyprotein with those of other sequenced members of the potyvirus group, including tobacco etch virus (TEV), tobacco vein mottling virus (TVMV), plum pox virus (PPV), and potato virus Y (PVY), allowed localization of putative protein cleavage sites. Read More

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November 1992

Nucleotide sequence of the 3'-terminal region of potato virus A RNA.

Virus Res 1992 Apr;23(1-2):99-105

Institute of Biotechnology, University of Helsinki, Finland.

The sequence of the 3'-terminal region of the genome of the potato virus A (PVA) was obtained from two independent cDNA clones. This sequence is 1383 nucleotides long and contains an open reading frame of 1178 nucleotides, ending with the translation termination codon TAA and followed by untranslated region of 205 nucleotides. Since the N-terminal amino acid of the coat protein of PVA was blocked, the position of the putative coat protein cleavage site has been deduced by searching for consensus sequences and by the analogy to other potyviruses. Read More

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Effects of antisense oligodeoxynucleotide hybridization on in vitro translation of potato virus Y RNA.

Virus Genes 1992 Jan;6(1):33-46

Station de Phytopathologie, Institut National de la Recherche Agronomique, Versailles, France.

Potato virus Y (PVY), a potyvirus, has an RNA genome containing 9704 nucleotides of which 185 belong to the 5' nontranslated region (NTR). Contrary to most eukaryotic mRNAs that have a cap structure, the potyvirus RNA has a genome-linked protein (VPg). In order to understand the mechanisms of PVY RNA translation initiation, hybrid-arrest translation was used to localize sequences involved in binding of proteins and/or ribosomes. Read More

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January 1992

[The antigenic characteristics of the capsid proteins in strains of the potato virus Y].

Nauchnye Doki Vyss Shkoly Biol Nauki 1991 (11):35-46

The antigenic properties of capsid proteins of potato virus Y (PVY) strains have been studied, the most wide antigenic specificity of necrotic group strains has been marked. Several antigen-active strains of necrotic group strains (PVY-N Far East, PVY-N Leningrad, PVY-N Moscow) and common group (PVY-O-3 Moscow, PVY-O Far East) have been revealed. Antisera against these strains reacted with any PVY strain. Read More

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February 1992
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