8,959 results match your criteria porcine ischemic


Nitrate-functionalized patch confers cardioprotection and improves heart repair after myocardial infarction via local nitric oxide delivery.

Nat Commun 2021 07 23;12(1):4501. Epub 2021 Jul 23.

State key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials (Ministry of Education), College of Life Sciences, Nankai University, Tianjin, China.

Nitric oxide (NO) is a short-lived signaling molecule that plays a pivotal role in cardiovascular system. Organic nitrates represent a class of NO-donating drugs for treating coronary artery diseases, acting through the vasodilation of systemic vasculature that often leads to adverse effects. Herein, we design a nitrate-functionalized patch, wherein the nitrate pharmacological functional groups are covalently bound to biodegradable polymers, thus transforming small-molecule drugs into therapeutic biomaterials. Read More

View Article and Full-Text PDF

A Cardiac Protection of Germinated Brown Rice During Cardiopulmonary Bypass Surgery and Simulated Myocardial Ischemia.

J Inflamm Res 2021 15;14:3307-3319. Epub 2021 Jul 15.

Department of Large Animal and Wildlife Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, KamphaengSaen Campus, Kamphaeng Saen, Thailand.

Purpose: The potential cardio-protective property of germinated brown rice (GBR) has been revealed by ameliorating risk factors related to cardiovascular diseases. This study hypothesized that the combination of GBR and cardioplegic solution could protect the cardiomyocytes exposed to simulated ischemic reperfusion injury in vitro study and preserve cardiac function during cardiopulmonary bypass surgery in animal models.

Methods: Primary porcine cardiomyocytes were isolated and experimented cell viability against simulated ischemic reperfusion injury. Read More

View Article and Full-Text PDF

Intraoperative quality assessment of tissue perfusion with indocyanine green (ICG) in a porcine model of mesenteric ischemia.

PLoS One 2021 20;16(7):e0254144. Epub 2021 Jul 20.

Department of Vascular Medicine, University Heart Center, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Background: Mesenteric ischemia is a severe and potentially lethal event. Assessment of intestine perfusion is eminently depending on the skills, and the experience of the surgeon. Thus, the therapy is biased by the right evaluation. Read More

View Article and Full-Text PDF

Antioxidant preconditioning attenuates liver ischemia/reperfusion injury after hepatectomy in swine.

J BUON 2021 May-Jun;26(3):1159-1164

6th Department of Surgery, Hygeia Hospital, 4, Erythrou Stavrou Str. and Kifisias, 15123, Marousi, Greece.

Purpose: The purpose of this study was to evaluate whether antioxidant preconditioning with Deferoxamine can attenuate liver ischemia reperfusion injury associated with extended hepatectomy in swine.

Methods: Eighteen swine were randomly assigned to two groups: Deferoxamine (DFO) and Surgery Only (SO). The animals in both groups were subjected to laparotomy, prolonged temporary occlusion of the right and middle hepatic pedicles and subsequent left hepatectomy. Read More

View Article and Full-Text PDF

Epinephrine plus chest compressions is superior to epinephrine alone in a hypoxia-induced porcine model of pseudo-pulseless electrical activity.

Resusc Plus 2021 Jun 2;6:100110. Epub 2021 Apr 2.

Department of Emergency Medicine, Dartmouth Hitchcock Medical Center, 1 Medical Center Dr #4B, Lebanon, NH 03756, USA.

Aim: Pseudo-pulseless electrical activity (pseudo-PEA) is a global hypotensive ischemic state with retained coordinated myocardial contractile activity and an organized ECG with no clinically detectable pulses. The role of standard external chest compressions (CPR) and its associated intrinsic hemodynamics remains unclear in the setting of pseudo-PEA. We undertook an experimental trial to compare epinephrine alone versus epinephrine with CPR in the treatment of pseudo-PEA. Read More

View Article and Full-Text PDF

Establishment of Carotid Artery Dissection and MRI Findings in a Swine Model.

Front Neurol 2021 16;12:669276. Epub 2021 Jun 16.

Department of Neurology, Southwest Hospital, Third Military Medical University, Chongqing, China.

Carotid artery dissection (CAD) is the leading cause of ischemic stroke in young patients; however, the etiology and pathophysiology of CAD remain largely unknown. In our study, two types of dissections (length × width: 1.5 cm × 1/3 circumference of intima, Group I, = 6; or 1. Read More

View Article and Full-Text PDF

Biochemical Studies in Perfundates and Homogenates of Isolated Porcine Kidneys after Flushing with Zinc or Zinc-Prolactin Modified Preservation Solution Using a Static Cold Storage Technique.

Molecules 2021 Jun 7;26(11). Epub 2021 Jun 7.

"Biochefa" Pharmaceutical Research and Production Plant, Kasztanowa 3, 41-200 Sosnowiec, Poland.

Zinc is an effective anti-inflammatory and antioxidant trace element. The aim of this study was to analyse the protective effect of zinc and zinc-prolactin systems as additives of preservation solutions in the prevention of nephron damage caused during ischemia. The study used a model for storing isolated porcine kidneys in Biolasol. Read More

View Article and Full-Text PDF

Gene therapy knockdown of Hippo signaling induces cardiomyocyte renewal in pigs after myocardial infarction.

Sci Transl Med 2021 06;13(600)

Texas Heart Institute, Houston, TX, USA.

Human heart failure, a leading cause of death worldwide, is a prominent example of a chronic disease that may result from poor cell renewal. The Hippo signaling pathway is an inhibitory kinase cascade that represses adult heart muscle cell (cardiomyocyte) proliferation and renewal after myocardial infarction in genetically modified mice. Here, we investigated an adeno-associated virus 9 (AAV9)-based gene therapy to locally knock down the Hippo pathway gene Salvador () in border zone cardiomyocytes in a pig model of ischemia/reperfusion-induced myocardial infarction. Read More

View Article and Full-Text PDF

Controlled Oxygenated Rewarming Compensates for Cold Storage-induced Dysfunction in Kidney Grafts.

Transplantation 2021 Jun 23. Epub 2021 Jun 23.

Department for Surgical Research Clinic for General, Visceral and Transplantation Surgery, University Hospital Essen.

Background: Normothermic machine perfusion (NMP) provides a promising strategy for preservation and conditioning of marginal organ grafts. However, at present high logistic effort limits normothermic renal perfusion to a short, postponed machine perfusion at site of the recipient transplant center. Thus, organ preservation during transportation still takes place under hypothermic conditions, leading to significantly reduced efficacy of NMP. Read More

View Article and Full-Text PDF

Effects of Sodium Thiosulfate During Resuscitation from Trauma-and-Hemorrhage in Cystathionine γ-Lyase (CSE) Knockout Mice.

Shock 2021 Jun 24. Epub 2021 Jun 24.

Institut für Anästhesiologische Pathophysiologie und Verfahrensentwicklung, Universitätsklinikum, Ulm, Germany Klinik für Psychosomatische Medizin und Psychotherapie, Universitätsklinikum, Ulm, Germany Klinik für Anästhesiologie und Intensivmedizin, Universitätsklinikum, Ulm, Germany Faculty of Science, York University, Toronto, ON, Canada.

Background: Sodium thiosulfate (Na2S2O3) is a clinically established drug with antioxidant and sulfide releasing properties. Na2S2O3 mediated neuro- and cardioprotective effects in ischemia/reperfusion models and anti-inflammatory effects in LPS-induced acute lung injury. Moreover, Na2S2O3 improved lung function during resuscitation from hemorrhagic shock in swine with pre-existing atherosclerosis, characterized by decreased expression of cystathionine γ-lyase (CSE), a major source of hydrogen sulfide (H2S) synthesis in the vasculature. Read More

View Article and Full-Text PDF

Histopathological Validation of Dark-Blood Late Gadolinium Enhancement MRI Without Additional Magnetization Preparation.

J Magn Reson Imaging 2021 Jun 24. Epub 2021 Jun 24.

Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands.

Background: Conventional bright-blood late gadolinium enhancement (LGE) cardiac magnetic resonance imaging (MRI) often suffers from poor scar-to-blood contrast due to the bright blood pool adjacent to the enhanced scar tissue. Recently, a dark-blood LGE method was developed which increases scar-to-blood contrast without using additional magnetization preparation.

Purpose: We aim to histopathologically validate this dark-blood LGE method in a porcine animal model with induced myocardial infarction (MI). Read More

View Article and Full-Text PDF

Ischemia-reperfusion injury to coronary arteries: Comprehensive microscopic study after reperfused myocardial infarction.

Ann Anat 2021 Nov 16;238:151785. Epub 2021 Jun 16.

INCLIVA Health Research Institute, Valencia, Spain; Department of Medicine, School of Medicine, University of Valencia, Valencia, Spain; Centro de Investigación Biomédica en Red - Cardiovascular, Spain; Cardiology Department, Hospital Clinico Universitario, Valencia, Spain. Electronic address:

Background: Coronary arteries supply oxygen and nutrients to the heart. We evaluated the dynamics of microscopic damage throughout the ischemia-reperfusion process in the wall of coronary arteries following myocardial infarction (MI).

Methods: In a swine model of reperfused MI, animals were divided into one control and four MI groups: 90-min ischemia without reperfusion, or followed by one minute, one week or one month reperfusion. Read More

View Article and Full-Text PDF
November 2021

Rapid custom prototyping of soft poroelastic biosensor for simultaneous epicardial recording and imaging.

Nat Commun 2021 06 17;12(1):3710. Epub 2021 Jun 17.

School of Mechanical Engineering, Purdue University, West Lafayette, IN, USA.

The growing need for the implementation of stretchable biosensors in the body has driven rapid prototyping schemes through the direct ink writing of multidimensional functional architectures. Recent approaches employ biocompatible inks that are dispensable through an automated nozzle injection system. However, their application in medical practices remains challenged in reliable recording due to their viscoelastic nature that yields mechanical and electrical hysteresis under periodic large strains. Read More

View Article and Full-Text PDF

Pulseless Electrical Activity as the Initial Cardiac Arrest Rhythm: Importance of Preexisting Left Ventricular Function.

J Am Heart Assoc 2021 Jul 14;10(13):e018671. Epub 2021 Jun 14.

Division of Cardiology Department of Medicine Johns Hopkins University Baltimore MD.

Background Pulseless electrical activity (PEA) is a common initial rhythm in cardiac arrest. A substantial number of PEA arrests are caused by coronary ischemia in the setting of acute coronary occlusion, but the underlying mechanism is not well understood. We hypothesized that the initial rhythm in patients with acute coronary occlusion is more likely to be PEA than ventricular fibrillation in those with prearrest severe left ventricular dysfunction. Read More

View Article and Full-Text PDF

Tapping on Pulseless Electrical Activity: An Opportunity for Improving Resuscitation Outcomes?

Authors:
Raúl J Gazmuri

J Am Heart Assoc 2021 Jul 14;10(13):e021798. Epub 2021 Jun 14.

Resuscitation Institute Rosalind Franklin University of Medicine and Science North Chicago IL.

View Article and Full-Text PDF

Changes in High-Frequency Intracardiac Electrogram Indicate Cardiac Ischemia.

J Cardiovasc Transl Res 2021 Jun 11. Epub 2021 Jun 11.

Department of Cardiology, Shaare Zedek Medical Center, Jerusalem, Israel.

High-frequency QRS (HFQRS) analysis of surface ECG is a reliable marker of cardiac ischemia (CI). This study aimed to assess the response of HFQRS signals from standard intracardiac electrodes (iHFQRS) to CI in swine and compare them with conventional ST-segment deviations. Devices with three intracardiac leads were implanted in three swine in a controlled environment. Read More

View Article and Full-Text PDF

Therapeutic efficacy of large aligned cardiac tissue derived from induced pluripotent stem cell in a porcine ischemic cardiomyopathy model.

J Heart Lung Transplant 2021 Aug 8;40(8):767-777. Epub 2021 May 8.

Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Osaka, Japan. Electronic address:

Background: Although induced pluripotent stem (iPS) cell-derived cardiac constructs may have a potential in cardiomyogenesis of a distressed myocardium, obtaining polarity in cardiac constructs, such as via myocyte alignment, may be crucial to achieve a maximum contractile force for better clinical outcomes. We herein hypothesized that transplantation of an aligned cardiac tissue derived from iPS cells has therapeutic effects in a porcine ischemic cardiomyopathy model as a preclinical trial.

Methods: Aligned cardiac tissues were developed by culturing high-purity iPS cell-derived cardiomyocytes in xeno-free conditions and transplanting them into infarct porcine hearts (iPS-CM group, n = 7; control, n = 6). Read More

View Article and Full-Text PDF

Downstream Paclitaxel Released Following Drug-Coated Balloon Inflation and Distal Limb Wound Healing in Swine.

JACC Basic Transl Sci 2021 May 31;6(5):416-427. Epub 2021 Mar 31.

CRF Skirball Center for Innovation, Orangeburg, New York, USA.

The authors evaluated the presence of paclitaxel and healing of distal hind limb wounds created in 27 swine using biopsy punches followed by paclitaxel-coated balloon (PCB) use in the iliofemoral arteries of healthy swine. After 14 and 28 days, no differences were seen in time course, appearance, and histopathology of wound healing between the single or triple PCB and uncoated balloon treatment despite clinically relevant paclitaxel concentrations in the skin adjacent to the healing wounds. Presence of paclitaxel downstream from the PCB treatment site does not impair the wound healing response of preexisting distal cutaneous lesions in healthy swine. Read More

View Article and Full-Text PDF

miRNA in cardiac development and regeneration.

Cell Regen 2021 Jun 1;10(1):14. Epub 2021 Jun 1.

Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, 200092, P.R. China.

Ischemic heart disease is one of the main causes of morbidity and mortality in the world. In adult mammalian hearts, most cardiomyocytes are terminally differentiated and have extremely limited capacity of proliferation, making it impossible to regenerate the heart after injuries such as myocardial infarction. MicroRNAs (miRNAs), a class of non-coding single-stranded RNA, which are involved in mRNA silencing and the regulation of post-transcriptional gene expression, have been shown to play a crucial role in cardiac development and cardiomyocyte proliferation. Read More

View Article and Full-Text PDF

A Swine Hind Limb Ischemia Model Useful for Testing Peripheral Artery Disease Therapeutics.

J Cardiovasc Transl Res 2021 May 28. Epub 2021 May 28.

Division of Cardiology, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA.

Currently, there is no large animal model of sustained limb ischemia suitable for testing novel angiogenic therapeutics for peripheral artery disease (PAD) such as drugs, genes, materials, or cells. We created a large animal model suitable for efficacy assessment of these therapies by testing 3 swine hind limb ischemia (HLI) variations and quantifying vascular perfusion, muscle histology, and limb function. Ligation of the ipsilateral external and bilateral internal iliac arteries produced sustained gait dysfunction compared to isolated external iliac or unilateral external and internal iliac artery ligations. Read More

View Article and Full-Text PDF

Normothermic Ex-vivo Kidney Perfusion in a Porcine Auto-Transplantation Model Preserves the Expression of Key Mitochondrial Proteins: An Unbiased Proteomics Analysis.

Mol Cell Proteomics 2021 May 23;20:100101. Epub 2021 May 23.

Toronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada; Division of Nephrology, Department of Medicine, Toronto General Hospital, University Health Network, University of Toronto, Toronto, Ontario, Canada; Soham and Shaila Ajmera Family Transplant Centre, Toronto General Hospital, University Health Network, Toronto, Ontario, Canada; Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada. Electronic address:

Normothermic ex-vivo kidney perfusion (NEVKP) results in significantly improved graft function in porcine auto-transplant models of donation after circulatory death injury compared with static cold storage (SCS); however, the molecular mechanisms underlying these beneficial effects remain unclear. We performed an unbiased proteomics analysis of 28 kidney biopsies obtained at three time points from pig kidneys subjected to 30 min of warm ischemia, followed by 8 h of NEVKP or SCS, and auto-transplantation. 70/6593 proteins quantified were differentially expressed between NEVKP and SCS groups (false discovery rate < 0. Read More

View Article and Full-Text PDF

Remote ischemic preconditioning improves tissue oxygenation in a porcine model of controlled hemorrhage without fluid resuscitation.

Sci Rep 2021 May 24;11(1):10808. Epub 2021 May 24.

Institute for Research in Military Medicine (IRMM), Faculty of Medicine, The Hebrew University of Jerusalem and the Israel Defense Forces Medical Corps, POB 12272, 91120, Jerusalem, Israel.

Remote ischemic preconditioning (RIPC) involves deliberate, brief interruptions of blood flow to increase the tolerance of distant critical organs to ischemia. This study tests the effects of limb RIPC in a porcine model of controlled hemorrhage without replacement therapy simulating an extreme field situation of delayed evacuation to definitive care. Twenty-eight pigs (47 ± 6 kg) were assigned to: (1) control, no procedure (n = 7); (2) HS = hemorrhagic shock (n = 13); and (3) RIPC + HS = remote ischemic preconditioning followed by hemorrhage (n = 8). Read More

View Article and Full-Text PDF

Real-time visualization of renal microperfusion using laser speckle contrast imaging.

J Biomed Opt 2021 May;26(5)

Medical Centre Leeuwarden, Department of Intensive Care, Leeuwarden, The Netherlands.

Significance: Intraoperative parameters of renal cortical microperfusion (RCM) have been associated with postoperative ischemia/reperfusion injury. Laser speckle contrast imaging (LSCI) could provide valuable information in this regard with the advantage over the current standard of care of being a non-contact and full-field imaging technique.

Aim: Our study aims to validate the use of LSCI for the visualization of RCM on ex vivo perfused human-sized porcine kidneys in various models of hemodynamic changes. Read More

View Article and Full-Text PDF

Preclinical trial of a MAP4K4 inhibitor to reduce infarct size in the pig: does cardioprotection in human stem cell-derived myocytes predict success in large mammals?

Basic Res Cardiol 2021 05 20;116(1):34. Epub 2021 May 20.

National Heart and Lung Institute, Imperial College London, Du Cane Road, London, W12 0NN, UK.

Reducing infarct size (IS) by interfering with mechanisms for cardiomyocyte death remains an elusive goal. DMX-5804, a selective inhibitor of the stress-activated kinase MAP4K4, suppresses cell death in mouse myocardial infarction (MI), human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs), and 3D human engineered heart tissue, whose fidelity to human biology is hoped to strengthen the route to clinical success. Here, DMX-10001, a soluble, rapidly cleaved pro-drug of DMX-5804, was developed for i. Read More

View Article and Full-Text PDF

End-Tidal Carbon Dioxide as an Indicator of Partial REBOA and Distal Organ Metabolism in Normovolemia and Hemorrhagic Shock in Anesthetized Pigs.

Shock 2021 May 19. Epub 2021 May 19.

Department of Cardiothoracic and Vascular Surgery, Faculty of Medicine and Health, Örebro University, Örebro, Sweden Centre for Clinical Research and Education, County Council of Värmland, Karlstad, Sweden School of Health Sciences, Örebro University, Örebro, Sweden Department of Vascular Surgery and Institute of Medicine, Department of Molecular and Clinical Medicine, Sahlgrenska University Hospital and Academy, Gothenburg, Sweden Department of Surgery, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.

Introduction: It is difficult to estimate the ischemic consequences when using partial resuscitative endovascular balloon occlusion of the aorta (REBOA). The aim was to investigate if end-tidal carbon dioxide (ETCO2) is correlated to degree of aortic occlusion, measured as distal aortic blood flow, and distal organ metabolism, estimated as systemic oxygen consumption (VO2), in a porcine model of normovolemia and hemorrhagic shock.

Materials And Methods: Nine anesthetized pigs (25-32 kg) were subjected to incremental steps of zone 1 aortic occlusion (reducing distal aortic blood flow by 33%, 66% and 100%) during normovolemia and hemorrhagic grade IV shock. Read More

View Article and Full-Text PDF

Metabolic MRI with hyperpolarized [1-C]pyruvate separates benign oligemia from infarcting penumbra in porcine stroke.

J Cereb Blood Flow Metab 2021 May 20:271678X211018317. Epub 2021 May 20.

The MR Research Center, Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.

Acute ischemic stroke patients benefit from reperfusion in a short time-window after debut. Later treatment may be indicated if viable brain tissue is demonstrated and this outweighs the inherent risks of late reperfusion. Magnetic resonance imaging (MRI) with hyperpolarized [1-C]pyruvate is an emerging technology that directly images metabolism. Read More

View Article and Full-Text PDF

Pretreatment with Propofol Reduces Pulmonary Injury in a Pig Model of Intestinal Ischemia-Reperfusion via Suppressing the High-Mobility Group Box 1 Protein (HMGB1)/Toll-Like Receptor 4 (TLR4)/Protein Kinase R (PKR) Signaling Pathway.

Med Sci Monit 2021 May 19;27:e930478. Epub 2021 May 19.

Department of Anesthesiology, Fifth People's Hospital, Fudan University, Shanghai, China (mainland).

BACKGROUND Propofol improves rodent pulmonary injury after intestinal ischemia-reperfusion (IIR). However, its effect and underlying mechanisms in large animals remain unclear. Here, we examined whether pretreatment with propofol could relieve lung injury during IIR in pigs, then investigated the underlying mechanism. Read More

View Article and Full-Text PDF

Intravenous Interferon-β1a for the Treatment of Ischemia-Reperfusion Injury in Acute Myocardial Infarct in Pigs.

Heart Surg Forum 2021 Apr 27;24(2):E409-E413. Epub 2021 Apr 27.

Heart Center, Turku University Hospital and University of Turku, Turku, Finland.

Background: To investigate the potential of intravenously administered porcine recombinant interferon-β1a (IFN-β1a) for myocardial protection during acute ischemia-reperfusion (IR) injury in an experimental animal model.

Methods: Twenty-two piglets (mean ± standard deviation, 26.7 ± 1. Read More

View Article and Full-Text PDF

Towards rapid intraoperative axial localization of spinal cord ischemia with epidural diffuse correlation monitoring.

PLoS One 2021 10;16(5):e0251271. Epub 2021 May 10.

Department of Anesthesiology and Pain Management, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.

Spinal cord ischemia leads to iatrogenic injury in multiple surgical fields, and the ability to immediately identify onset and anatomic origin of ischemia is critical to its management. Current clinical monitoring, however, does not directly measure spinal cord blood flow, resulting in poor sensitivity/specificity, delayed alerts, and delayed intervention. We have developed an epidural device employing diffuse correlation spectroscopy (DCS) to monitor spinal cord ischemia continuously at multiple positions. Read More

View Article and Full-Text PDF

Multimodal Measurements of Brain Tissue Metabolism and Perfusion in a Neonatal Model of Hypoxic-Ischaemic Injury.

Adv Exp Med Biol 2021 ;1269:203-208

Biomedical Optics Research Laboratory, University College London, London, UK.

This is the first multimodal study of cerebral tissue metabolism and perfusion post-hypoxic-ischaemic (HI) brain injury using broadband near-infrared spectroscopy (bNIRS), diffuse correlation spectroscopy (DCS), positron emission tomography (PET) and magnetic resonance spectroscopy (MRS). In seven piglet preclinical models of neonatal HI, we measured cerebral tissue saturation (StO), cerebral blood flow (CBF), cerebral oxygen metabolism (CMRO), changes in the mitochondrial oxidation state of cytochrome c oxidase (oxCCO), cerebral glucose metabolism (CMRglc) and tissue biochemistry (Lac+Thr/tNAA). At baseline, the parameters measured in the piglets that experience HI (not controls) were 64 ± 6% StO, 35 ± 11 ml/100 g/min CBF and 2. Read More

View Article and Full-Text PDF