mBio 2017 03 21;8(2). Epub 2017 Mar 21.
Department of Plant Pathology and Microbiology, University of California, Riverside, California, USA
Dicer enzymes process virus-specific double-stranded RNA (dsRNA) into small interfering RNAs (siRNAs) to initiate specific antiviral defense by related RNA interference (RNAi) pathways in plants, insects, nematodes, and mammals. Antiviral RNAi in requires Dicer-related helicase 1 (DRH-1), not found in plants and insects but highly homologous to mammalian retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs), intracellular viral RNA sensors that trigger innate immunity against RNA virus infection. However, it remains unclear if DRH-1 acts analogously to initiate antiviral RNAi in Here, we performed a forward genetic screen to characterize antiviral RNAi in Using a mapping-by-sequencing strategy, we uncovered four loss-of-function alleles of , three of which caused mutations in the helicase and C-terminal domains conserved in RLRs. Read More