7 results match your criteria population vsirnas

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RIG-I Homolog Mediates Antiviral RNA Interference Downstream of Dicer-Dependent Biogenesis of Viral Small Interfering RNAs.

mBio 2017 03 21;8(2). Epub 2017 Mar 21.

Department of Plant Pathology and Microbiology, University of California, Riverside, California, USA

Dicer enzymes process virus-specific double-stranded RNA (dsRNA) into small interfering RNAs (siRNAs) to initiate specific antiviral defense by related RNA interference (RNAi) pathways in plants, insects, nematodes, and mammals. Antiviral RNAi in requires Dicer-related helicase 1 (DRH-1), not found in plants and insects but highly homologous to mammalian retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs), intracellular viral RNA sensors that trigger innate immunity against RNA virus infection. However, it remains unclear if DRH-1 acts analogously to initiate antiviral RNAi in Here, we performed a forward genetic screen to characterize antiviral RNAi in Using a mapping-by-sequencing strategy, we uncovered four loss-of-function alleles of , three of which caused mutations in the helicase and C-terminal domains conserved in RLRs. Read More

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Viral small RNAs reveal the genomic variations of three grapevine vein clearing virus quasispecies populations.

Virus Res 2017 02 21;229:24-27. Epub 2016 Dec 21.

Center for Grapevine Biotechnology, W.H. Darr College of Agriculture, Missouri State University, Mountain Grove, MO 65711, USA. Electronic address:

Viral small RNAs (vsRNAs) include viral small interfering RNAs (vsiRNAs) that are initiators and products of RNA silencing, and small RNAs that are derived from viral RNAs with function still unknown. Sequencing of vsRNAs allows assembling of viral genomes and revelation of viral population variations at genomic levels. Grapevine vein clearing virus (GVCV) is a new member of the family Caulimoviridae whose DNA genome is replicated by reverse transcription of pre-genomic RNA molecules. Read More

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February 2017

Characterization of virus-derived small interfering RNAs in Apple stem grooving virus-infected in vitro-cultured Pyrus pyrifolia shoot tips in response to high temperature treatment.

Virol J 2016 10 6;13(1):166. Epub 2016 Oct 6.

State Key Laboratory of Agricultural Microbiology, Wuhan, Hubei, 430070, People's Republic of China.

Background: Heat treatment (known as thermotherapy) together with in vitro culture of shoot meristem tips is a commonly used technology to obtain virus-free germplasm for the effective control of virus diseases in fruit trees. RNA silencing as an antiviral defense mechanism has been implicated in this process. To understand if high temperature-mediated acceleration of the host antiviral gene silencing system in the meristem tip facilitates virus-derived small interfering RNAs (vsiRNA) accumulation to reduce the viral RNA titer in the fruit tree meristem tip cells, we used the Apple stem grooving virus (ASGV)-Pyrus pyrifolia pathosystem to explore the possible roles of vsiRNA in thermotherapy. Read More

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October 2016

Cotton Leaf Curl Multan Virus-Derived Viral Small RNAs Can Target Cotton Genes to Promote Viral Infection.

Front Plant Sci 2016 4;7:1162. Epub 2016 Aug 4.

Jiangsu Key Laboratory for Bioresources of Saline Soils, Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences Nanjing, China.

RNA silencing is a conserved mechanism in plants that targets viruses. Viral small RNAs (vsiRNAs) can be generated from viral double-stranded RNA replicative intermediates within the infected host, or from host RNA-dependent RNA polymerases activity on viral templates. The abundance and profile of vsiRNAs in viral infections have been reported previously. Read More

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Comparative analysis of virus-specific small RNA profiles of three biologically distinct strains of Potato virus Y in infected potato (Solanum tuberosum) cv. Russet Burbank.

Virus Res 2014 Oct 15;191:153-60. Epub 2014 Jul 15.

Department of Plant Pathology, Washington State University, Pullman, WA, USA. Electronic address:

Deep sequencing technology has enabled the analysis of small RNA profiles of virus-infected plants and could provide insights into virus-host interactions. Potato virus Y is an economically important viral pathogen of potato worldwide. In this study, we investigated the nature and relative levels of virus-derived small interfering RNAs (vsiRNAs) in potato cv. Read More

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October 2014

Differential expression of tomato spotted wilt virus-derived viral small RNAs in infected commercial and experimental host plants.

PLoS One 2013 15;8(10):e76276. Epub 2013 Oct 15.

Queensland Alliance for Agriculture and Food Innovation, the University of Queensland, St. Lucia, Australia.

Background: Viral small RNAs (vsiRNAs) in the infected host can be generated from viral double-stranded RNA replicative intermediates, self-complementary regions of the viral genome or from the action of host RNA-dependent RNA polymerases on viral templates. The vsiRNA abundance and profile as well as the endogenous small RNA population can vary between different hosts infected by the same virus influencing viral pathogenicity and host response. There are no reports on the analysis of vsiRNAs of Tomato spotted wilt virus (TSWV), a segmented negative stranded RNA virus in the family Bunyaviridae, with two of its gene segments showing ambisense gene arrangement. Read More

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Population diversity of rice stripe virus-derived siRNAs in three different hosts and RNAi-based antiviral immunity in Laodelphgax striatellus.

PLoS One 2012 28;7(9):e46238. Epub 2012 Sep 28.

State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, China.

Background: Small RNA-mediated gene silencing plays evolutionarily conserved roles in gene regulation and defense against invasive nucleic acids. Virus-derived small interfering RNAs (vsiRNAs) are one of the key elements involved in RNA silencing-based antiviral activities in plant and insect. vsiRNAs produced after viruses infecting hosts from a single kingdom (i. Read More

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February 2013
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