597,849 results match your criteria polymerase chain

Long non-coding RNA LINC01003 suppresses the development of multiple myeloma by targeting miR-33a-5p/PIM1 axis.

Leuk Res 2021 Mar 31;106:106565. Epub 2021 Mar 31.

Department of Hematology, The First Affiliated Hospital of Anhui Medical University, No. 218, Jixi Road, Shushan District, Hefei City, Anhui Province, 230032, China. Electronic address:

Background: Numerous long non-coding RNAs (lncRNAs) are reported to affect the progression of multiple myeloma (MM). This study is aimed to explore the role and downstream mechanism of lncRNA LINC01003 in MM.

Materials And Methods: Xenograft tumor assay was used to assess the function of LINC01003 in MM in vivo. Read More

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MOL-PCR and xMAP technology - a multiplex system for fast detection of food- and water-borne viruses.

J Mol Diagn 2021 Apr 14. Epub 2021 Apr 14.

Department of Animal Origin Food and Gastronomic Sciences, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho tr. 1946/1, CZ61242 Brno, Czech Republic.

Viruses are common causes of food- and water-borne diseases worldwide. Conventional identification of these agents is based on cultivation, antigen detection, electron microscopy or real-time polymerase chain reaction. Since recent technological advancements in detection methods are focused on fast and robust analysis, a rapid multiplexing technology, which can detect a broad spectrum of pathogenic viruses connected to food or water contamination, was utilized. Read More

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Evaluation of NitroSpeed-Carba NP Test for rapid identification among different classes of carbapenemases in Enterobacterales and Pseudomonas aeruginosa.

Int J Infect Dis 2021 Apr 14. Epub 2021 Apr 14.

Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China. Electronic address:

Objectives: This study aimed to evaluate the performance of the NitroSpeed-Carba NP test for detecting carbapenemases in the clinical strains of Enterobacterales and Pseudomonas aeruginosa and analyze its advantages and limitations.

Methods: The antimicrobial susceptibility tests were performed according to the agar dilution method. Using the modified carbapenemase inactivation method (mCIM), polymerase chain reaction (PCR), and sequencing, the production of carbapenemase and the prevalence of genes were studied. Read More

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GAS5 regulates viability and apoptosis in TGF-β1-stimulated bronchial epithelial cells by regulating miR-217/HDAC4 axis.

Genes Genomics 2021 Apr 17. Epub 2021 Apr 17.

Department of Pediatrics, Heping Hospital Affiliated to Changzhi Medical College, No. 110, Yanan Sourth Road, Changzhi, 046000, Shanxi Province, China.

Background: Asthma is a serious respiratory disease that affects the physical and mental health of children. Airway epithelial apoptosis concomitantly mediated by transforming growth factor-β1 (TGF-β1) is a crucial component of asthma pathogenesis. LncRNA growth Arrest Specific 5 (GAS5), microRNA-217 (miR-217) and Histone deacetylase 4 (HDAC4) shown a close relationship with TGF-β1-induced injury of airway epithelial. Read More

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Comparative epidemiology and pathophysiology of patent and latent babesiosis caused by Babesia bigemina in buffaloes and cattle from different agroclimatic zones of Punjab State, India.

Trop Anim Health Prod 2021 Apr 17;53(2):264. Epub 2021 Apr 17.

Department of Veterinary Parasitology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, 141004, India.

To conduct comparative epidemiology of parasitologically positive (patent) and polymerase chain reaction positive (latent) cases of bovine babesiosis in Bet Region (low-lying areas adjoining Sutlej, Beas, Ravi, and Ghaggar rivers of Punjab) of diverse agroclimatic zones of Punjab state in relation to haematobiochemical parameters as patho-physiological markers, blood samples from 783 dairy animals (487 buffaloes and 296 cattle) were analysed parasitologically by Giemsa-stained blood smears (GSBS) and by molecular-based polymerase chain reaction (PCR) targeting SpeI-AvaI restriction fragment of Babesia bigemina. We ruled out the endemicity of the disease with 2.17% patent and 3. Read More

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Loss of microRNA-147 function alleviates synovial inflammation through ZNF148 in rheumatoid and experimental arthritis.

Eur J Immunol 2021 Apr 17. Epub 2021 Apr 17.

Institute of Molecular and Translational Medicine (IMTM), and Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, West Yanta Road No.76, Xi'an, Shaanxi, 710061, PR China.

MicroRNA-147 (miR-147) had been previously found induced in synoviocytes by inflammatory stimuli derived from T cells in experimental arthritis. This study was designed to verify whether loss of its function might alleviate inflammatory events in joints of experimental and rheumatoid arthritis (RA). DA rats were injected intradermally with pristane to induce arthritis, and rno-miR-147 antagomir was locally administrated into individual ankle compared with negative control or rno-miR-155-5p antagomir (potential positive control). Read More

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Circular RNA_0000190 and its target microRNA-767-5p are dysregulated, and they are related to risk stratification as well as prognosis in multiple myeloma patients.

Ir J Med Sci 2021 Apr 16. Epub 2021 Apr 16.

Department of Hematology, The First People's Hospital of Jingmen, No. 67 Xiangshan Avenue, Jingmen, 448000, People's Republic of, China.

Background: This study aimed to explore the correlation between circular RNA_0000190 (circ_0000190) and microRNA-767-5p (miR-767-5p), and their correlations with biochemical indices, risk stratification, treatment response, and survival in multiple myeloma (MM) patients.

Methods: Bone marrow (BM) plasma cells of 86 MM patients (during standard diagnostic procedures) and 30 healthy donors (HDs) (examination of the eligibility for BM transplantation) were obtained, among which circ_0000190 and miR-767-5p expressions were detected using reverse transcription quantitative polymerase chain reaction. In MM patients, Durie-Salmon stage and International Staging System (ISS) stage were assessed. Read More

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Rhabdomyolysis and Multisystem Organ Failure Due to Fulminant Ehrlichiosis Infection.

Wilderness Environ Med 2021 Apr 13. Epub 2021 Apr 13.

Saint Louis University School of Medicine, Division of Emergency Medicine, St. Louis, MO.

A previously healthy 51-y-old male presented to his local emergency department with subjective fevers, myalgias, dyspnea, and generalized weakness that had been progressive for several weeks. He was initially diagnosed with bilateral pneumonia, septic shock, and rhabdomyolysis requiring transfer to a tertiary care facility. He was treated for sepsis with broad-spectrum antibiotics, steroids, and a fluid bolus before transfer. Read More

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Relation of vitamin D and BsmI variant with temporomandibular diseases in the Turkish population.

Br J Oral Maxillofac Surg 2020 Aug 28. Epub 2020 Aug 28.

Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Gaziosmanpasa University, Tokat, Turkey. Electronic address:

Vitamin D (VD) levels and several variants in the vitamin D receptor (VDR) gene are associated with the occurrence of diseases of the bones and cartilage. The aim of this research was to study and compare the association of the BsmI variant in the VDR gene as well as VD levels in disc displacement with reduction (DDR) between patients and healthy controls. This was a case-control study, in which 104 patients of DDR and 102 healthy individuals were studied. Read More

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Innate immune response in bovine neutrophils stimulated with Mycoplasma bovis.

Vet Res 2021 Apr 16;52(1):58. Epub 2021 Apr 16.

Animal Health Laboratory, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, 069-8501, Japan.

Mycoplasma bovis (M. bovis) is a significant worldwide pathogen of cattle. Neutrophils have an important role in the innate immune response during infection with M. Read More

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Preparation and applications of Arabidopsis thaliana guard cell protoplasts.

New Phytol 2002 Mar 5;153(3):517-526. Epub 2002 Mar 5.

Biology Department, The Pennsylvania State University, 208 Mueller Laboratory, University Park, PA, USA, 16803.

•  Guard cells play an important role in the physiology and development of plants. The genetic resources available for Arabidopsis thaliana make it the most favorable plant species for the study of guard cell processes, but it is not easy to isolate highly purified preparations of large numbers of guard cells from this species. Here, we describe methods for isolation of both guard cell and mesophyll cell protoplasts from A. Read More

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Identification of endophytic fungi from Livistona chinensis based on morphology and rDNA sequences.

New Phytol 2000 Sep;147(3):617-630

1 Centre for Research in Fungal Diversity, Department of Ecology and Biodiversity, The University of Hong Kong, Pokfulam Road, Hong Kong.

A survey of the endophytic fungi in fronds of Livistona chinensis was carried out in Hong Kong. The endophyte assemblages identified using morphological characters consisted of 16 named species and 19 'morphospecies', the latter grouped based on cultural morphology and growth rates. Arrangement of taxa into morphospecies does not reflect species phylogeny, and therefore selected morphospecies were further identified based on ribosomal DNA (rDNA) sequence analysis. Read More

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September 2000

Association between active pulmonary tuberculosis and circulating microRNAs: A preliminary study from Turkey.

Turk J Med Sci 2021 Apr 17. Epub 2021 Apr 17.

Background/aim: Tuberculosis is a public health problem that still remains significant. For prevention, diagnosis and treatment on tuberculosis more effective novel biomarkers are needed. MicroRNAs can regulate innate and adaptive immune responses, alter host-pathogen interactions and affect progression of diseases. Read More

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Diagnostic accuracy of LAMP versus PCR over the course of SARS-CoV-2 infection.

Int J Infect Dis 2021 Apr 13. Epub 2021 Apr 13.

Department of Infectious Diseases, Fujita Health University School of Medicine, Toyoake, Aichi, Japan; Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.

Objective: Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been validated for diagnosis of several viral infections. However, its diagnostic accuracy in detecting SARS-CoV-2 in real-life clinical settings remains unclear. The aim of this study was to determine the diagnostic sensitivity and specificity of RT-LAMP compared to reverse transcription-quantitative polymerase chain reaction (RT-qPCR) over the disease course of COVID-19. Read More

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Corilagin alleviates hypertrophic scars via inhibiting the transforming growth factor (TGF)-β/Smad signal pathway.

Life Sci 2021 Apr 13:119483. Epub 2021 Apr 13.

Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address:

Aims: Exploring the effects of corilagin on hypertrophic scar (HS) and its underlying mechanisms.

Main Methods: Human HS-derived fibroblasts (HSFs) were isolated and treated with corilagin. To investigate the effects of corilagin on HSFs, quantitative real time polymerase chain reaction (qRT-PCR), western blotting, wound healing, and immunofluorescence assays were performed. Read More

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BMP9 Can Induce Schwann Cells Expressing Simian Virus 40 T Antigen to Differentiate into Fat and Bone and .

Cell Reprogram 2021 Apr;23(2):108-116

Department of Hepatobiliary Surgery, Zhongnan Hospital of Wuhan University, Wuhan, China.

In our previous study, we constructed Schwann cells (SCs) that stably express Simian virus 40 T antigen (SV40T-SCs). SV40T-SCs functions and markers are similar to those of neural crest cells. There we used bone morphogenetic protein 9 (BMP9) to induce SV40T-SCs differentiation and and study possible related mechanism. Read More

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Frequency of Growth Differentiation Factor 5 rs143383 and asporin D-repeat polymorphisms in patients with hand and knee osteoarthritis in Kurdistan province, Iran.

Int J Rheum Dis 2021 Apr 16. Epub 2021 Apr 16.

Cancer & Immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.

Aim: Osteoarthritis (OA) is the most common chronic joint disorder, resulting from the breakdown of joint cartilage. It occurs in the knees, hands, and hips, leading to pain, stiffness, inflammation, and swelling.

Methods: In this study, 100 hand and knee OA patients, meeting the American College of Rheumatology criteria were included in the case group, and 100 healthy individuals were allocated to the control group. Read More

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FRugally Optimized DNA Octomer (FRODO) qPCR Measurement of HIV and SIV in Human and Nonhuman Primate Samples.

Curr Protoc 2021 Apr;1(4):e93

Barrier Immunity Section, Laboratory of Viral Diseases, National Institutes of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, Maryland.

Quantitative polymerase chain reactions (qPCRs) are commonly employed to enumerate genes of interest among particular biological samples. Insertion of PCR amplicons into plasmid DNA is a mainstay for creation of known quantities of target sequences to standardize qPCRs. Typically, one amplicon is inserted into one plasmid construct, and the plasmid is then amplified, purified, serially diluted, and quantified to be used to enumerate target sequences in unknown samples. Read More

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M2a macrophage can rescue proliferation and gene expression of benign prostate hyperplasia epithelial and stroma cells from insulin-like growth factor 1 knockdown.

Prostate 2021 Apr 16. Epub 2021 Apr 16.

Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China.

Background: Benign prostatic hyperplasia (BPH) is a common disease in elderly men and is often accompanied by chronic inflammation. Macrophages (several subtypes) are the main inflammatory cells that infiltrate the hyperplastic prostate and are found to secrete cytokines and growth factors. The current study aims to explore the effect of M2a macrophages on the development of BPH via insulin-like growth factor 1 (IGF-1). Read More

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Challenges and new methods in the diagnosis of Lyme disease in children.

Przegl Epidemiol 2020 ;74(4):652-661

Medical University of Bialystok, Department of Pediatric Infectious Diseases.

Despite the constant development of biotechnology, laboratory diagnostics of Lyme disease in children still poses a significant challenge. The aim of this article is to present the current methods of Lyme disease diagnosis and its future perspectives. A serological test is often the first step in supporting clinical diagnosis of Lyme disease in children. Read More

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January 2020

Transcriptomic analysis of diapause-associated genes in Exorista civilis Rondani (Diptera:Tachinidae).

Arch Insect Biochem Physiol 2021 Apr 16:e21789. Epub 2021 Apr 16.

Research Center for Quality, Safety and Risk Assessment of Grass and Livestock Products, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, China.

Exorista civilis Rondani (Diptera:Tachinidae) is an excellent dominant parasitic enemy all over the world. But there has been a lack of research on the molecular regulation of diapause in E. civilis. Read More

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Risk factors for infestation by Triatoma dimidiata in a rural locality of Veracruz, Mexico, with active transmission of Trypanosoma cruzi: weather and rain as factors.

Trop Med Int Health 2021 Apr 15. Epub 2021 Apr 15.

LADISER Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Universidad Veracruzana, Veracruz, México.

Objective: To analyze the ecological and social factors involved in infestation of houses by T. dimidiata in a rural locality of Veracruz, Mexico, where active transmission of the parasite is occurring.

Methods: A survey was applied to the households of the locality to obtain sociodemographic data. Read More

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COVID-19-related refractory status epilepticus with the presence of SARS-CoV-2 (RNA) in the CSF: a case report.

Neurol Sci 2021 Apr 15. Epub 2021 Apr 15.

Department of Infectious Disease, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Amongst the neurologic complications of COVID-19 disease, very few reports have shown the presence of the virus in the cerebrospinal fluid (CSF). Seizure and rarely status epilepticus can be associated with COVID-19 disease. Here we present a 73-year-old male with prior history of stroke who has never experienced seizure before. Read More

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Identification and functional characterization of a new flavonoid synthase gene MdFLS1 from apple.

Planta 2021 Apr 15;253(5):105. Epub 2021 Apr 15.

School of Pharmacy, Liaocheng University, Liaocheng, Shandong, 250000, People's Republic of China.

Main Conclusion: The flavonoid synthase gene MdFLS1 from apple, which possibly plays an important role in anthocyanin synthesis, accumulates in the purple-red branches of Malus 'Pink spire'. Flavonoid metabolism serves an important function in plant growth and development. In this study, we selected 20 varieties of apple lines, 10 green and ten red branches, from the plant nursery of Qingdao Agriculture Academy. Read More

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Transcriptional Enhancer Factor Domain Family member 4 Exerts an Oncogenic Role in Hepatocellular Carcinoma by Hippo-Independent Regulation of Heat Shock Protein 70 Family Members.

Hepatol Commun 2021 Apr 20;5(4):661-674. Epub 2021 Jan 20.

Institute of Medical Genetics and Pathology University Hospital Basel Basel Switzerland.

Transcriptional enhancer factor domain family member 4 (TEAD4) is a downstream effector of the conserved Hippo signaling pathway, regulating the expression of genes involved in cell proliferation and differentiation. It is up-regulated in several cancer types and is associated with metastasis and poor prognosis. However, its role in hepatocellular carcinoma (HCC) remains largely unexplored. Read More

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Digital next-generation sequencing of cell-free DNA for pancreatic cancer.

JGH Open 2021 Apr 22;5(4):508-516. Epub 2021 Mar 22.

First Department of Internal Medicine, Faculty of Medicine University of Yamanashi Chuo Japan.

Background And Aim: The clinical applicability of digital next-generation sequencing (dNGS), which eliminates polymerase chain reaction (PCR) and sequencing error-derived noise by using molecular barcodes (MBs), has not been fully evaluated. We evaluated the utility of dNGS of cell-free DNA (cfDNA) in liquid biopsies obtained from patients with pancreatic cancer.

Methods: Fifty-eight patients with pancreatic cancer undergoing endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) were included. Read More

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Phylogenetic studies of Newcastle disease virus isolated from poultry flocks in South Sulawesi Province, Indonesia, in 2019.

J Adv Vet Anim Res 2021 Mar 7;8(1):129-137. Epub 2021 Mar 7.

Department of Animal Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia.

Objective: Indonesia is one of the Newcastle disease (ND) endemic countries in the world. An outbreak of the ND virus (NDV) was first reported in Indonesia in 1926. This study aimed to detect, isolate, and classify the NDV by molecular approaches from poultry farms in South Sulawesi Province of Indonesia in 2019. Read More

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Infectious bronchitis associated with infection in commercial broiler chickens: a case report.

J Adv Vet Anim Res 2021 Mar 5;8(1):101-104. Epub 2021 Mar 5.

Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Pengkalan Chepa, 16100 Kota Bharu, Kelantan, Malaysia.

Objective: In this case report, we have investigated the infectious bronchitis (IB) virus (IBV) outbreak with the co-infection of in 28-33-day-old broiler chickens in Malaysia.

Materials And Methods: A farmer complained that Cobb 500 chickens, raised in the open house, were having bloody diarrhea, open mouth breathing, non-uniform growth, and ruffled feathers. The mortality was about 100 birds (from about 7000 birds) per day. Read More

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Isolation and detection of antibiotics resistance genes of from broiler farms in Sukabumi, Indonesia.

J Adv Vet Anim Res 2021 Mar 5;8(1):84-90. Epub 2021 Mar 5.

Department of Animal Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia.

Objective: This study aimed to isolate and identify from broiler samples from Sukabumi, Indonesia. Also, antibiogram studies of the isolated bacteria were carried out considering the detection of the antibiotic resistance genes.

Materials And Methods: Cloaca swabs ( = 45) were collected from broilers in Sukabumi, Indonesia. Read More

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Isolation of multidrug-resistant from turkeys in Dinajpur, Bangladesh, and their antibiogram profile.

J Adv Vet Anim Res 2021 Mar 5;8(1):64-69. Epub 2021 Mar 5.

Animal Biotechnology Division, National Institute of Biotechnology, Ashulia, Bangladesh.

Objective: The study was carried out for molecular characterization and antibiotic resistance analysis of isolated from different turkey farms in Dinajpur, Bangladesh.

Materials And Methods: A total of 45 samples comprising feces ( = 23) and cloacal swabs ( = 22) were collected randomly from turkeys. The samples were subjected to isolation and identification of by cultural and biochemical characteristics, followed by polymerase chain reaction and sequencing. Read More

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