Biomed Mater 2021 Jun 11. Epub 2021 Jun 11.
Department of Chemical Engineering, Indian Institute of Technology Hyderabad, IITH Main Road, Near NH 65, Kandi, Sangareddy, Hyderabad, Telanagana, Hyderabad, 502205, INDIA.
In this study, we offer new insights into the contrasting effects of electrospun fiber orientation on microglial polarization under normoxia and hypoxia and establish for the first time, the intrinsically protective roles of electrospun meshes against hypoxia-induced microglial responses. First, resting microglia were cultured under normoxia on poly(caprolactone) (PCL) fibers possessing two distinctly different fiber orientations. Matrix-guided differences in cell shape/orientation and differentially expressed Rho GTPases (RhoA, Rac1, Cdc42) were well-correlated with the randomly oriented fibers inducing a predominantly pro-inflammatory phenotype and the aligned fibers sustaining a resting phenotype. Read More