5,410 results match your criteria pol iii


Platelet function in patients undergoing surgical and transcatheter aortic valve replacement: a comparative study.

Kardiol Pol 2021 ;79(5):554-561

1st Department of Cardiology, Poznan University of Medical Sciences, Poznań, Poland.

Background: Intervention-induced platelet hypercoagulability may pose a risk of serious adverse events for patients.

Aims: This study aimed to assess whether surgical and transcatheter aortic valve replacement (SAVR and TAVR) differ in periprocedural platelet activity.

Methods: The total number of 24 patients with a mean age (SD) of 71 (13) years who underwent SAVR (n = 12) or TAVR (n = 12) were recruited for the study. Read More

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January 2021

Rewiring of lactate-IL-1β auto-regulatory loop with Clock-Bmal1: A feed-forward circuit in glioma.

Mol Cell Biol 2021 Jun 14:MCB0044920. Epub 2021 Jun 14.

National Brain Research Centre, Manesar, Haryana 122 052, India.

De-synchronized circadian rhythm in tumors is coincident with aberrant inflammation and dysregulated metabolism. As their inter-relationship in cancer etiology is largely unknown, we investigated the link between the three in glioma. Tumor metabolite lactate- mediated increase in pro-inflammatory cytokine IL-1β was concomitant with elevated levels of core circadian regulators Clock and Bmal1. Read More

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Efficient genome editing using endogenous U6 snRNA promoter-driven CRISPR/Cas9 sgRNA in Sclerotinia sclerotiorum.

Fungal Genet Biol 2021 Jun 10:103598. Epub 2021 Jun 10.

Department of Plant Pathology, 1450 Fifield Hall, University of Florida, Gainesville, Florida, USA. Electronic address:

We previously reported on a CRISPR-Cas9 genome editing system for the necrotrophic fungal plant pathogen Sclerotinia sclerotiorum. This system (the TrpC-sgRNA system), based on an RNA polymerase II (RNA Pol II) promoter (TrpC) to drive sgRNA transcription in vivo, was successful in creating gene insertion mutants. However, relatively low efficiency targeted gene editing hampered the application of this method for functional genomic research in S. Read More

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Diagnosing antenatal fetal distress.

Ginekol Pol 2021 Jun 9. Epub 2021 Jun 9.

Erzincan Binali Yildirim University Mengucek Gazi Training and Research Hospital, Department of Gynecology and Obstetrics, Turkey.

Objectives: The values of acceleration capacity and deceleration capacity are known to capture fetal neurological development. The fetal growth restriction was found to be featured by decreased variables of phase rectified signal averaging. We have speculated that acceleration capacity and deceleration capacity could be of use in the detection of antenatal fetal distress during fetal growth restriction. Read More

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Impact of clinicopathological variables on laparoscopic hysterectomy complications, a tertiary center experience.

Ginekol Pol 2021 Jun 9. Epub 2021 Jun 9.

Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.

Objectives: To analyze intraoperative and postoperative complications according to Clavian-Dindo Classification (CDC) and evaluate the influence of clinicopathological features on the feasibility and safety of total laparoscopic hysterectomy (TLH) in patients that underwent surgery in a tertiary center.

Material And Methods: We retrospectively reviewed the database of 469 patients that underwent surgery for patients who underwent extra facial TLH from 2013 to 2020.

Results: A total of 86 (18. Read More

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[The SAGA Deubiquitinilation (DUB) Module Participates in Pol III-Dependent Transcription].

Mol Biol (Mosk) 2021 May-Jun;55(3):500-509

Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.

SAGA, the multicomponent complex responsible for acetylation of histone N-terminal lysine residues, is involved in the transcription activation of a wide range of eukaryote genes. SAGA contains a protein module, DUB, which is responsible for histone deubiquitination. In this paper we show that the DUB module may be found within cells independently of SAGA. Read More

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Obstructive oligospermia: the role of interventional radiology in its diagnosis and treatment.

Pol J Radiol 2021 12;86:e204-e207. Epub 2021 Apr 12.

Department of Advanced Biomedical Sciences, University Federico II of Naples (UNINA), Naples, Italy.

Purpose: According to the latest World Health Organization guidelines (2010), oligo-sperm it is due to a sperm concentration of less than 15 million/ml of seminal fluid. The cause can be obstructive and non-obstructive. Interventional radiology allows diagnosis and, in some cases, minimally invasive treatment. Read More

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The effect of miR-496 on eIF3h in lung invasive adenocarcinoma.

Authors:
Ji-Chun

Acta Biochim Pol 2021 May 26. Epub 2021 May 26.

Department of Cardiothoracic Surgery, Changzhou No. 2 People's Hospital, The Affiliated Hospital of Nanjing Medical University, Changzhou, Jiangsu 213000, P.R. China.

This study explores the correlation between microRNA-496 (miR-496) expression and eukaryotic translation initiation factor 3H (eIF3h) expression in lung invasive adenocarcinoma. A total of 71 patients with lung adenocarcinoma were included in the study. The tumor tissue samples were collected together with the normal lung tissue samples that were acquired more than 2 cm away from the edge of the tumor). Read More

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Polish adaptation of emotional Stroop test in assessment of pedophilia - a pilot study.

Psychiatr Pol 2021 Feb 28;55(1):85-100. Epub 2021 Feb 28.

Centrum Medyczne Kształcenia Podyplomowego, Klinika Psychiatrii.

Objectives: A pilot study was conducted in order to construct a Polish adaptation of emotional Stroop test in assessment of pedophilia.

Methods: The study consisted of two stages. The first stage involved creating test material by ranking words in adequate lists by competent experts. Read More

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February 2021

Upregulation of miR-1266-5p serves as a prognostic biomarker of hepatocellular carcinoma and facilitates tumor cell proliferation, migration and invasion.

Acta Biochim Pol 2021 May;68(2):293-300

Imaging Center, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong, Chin.

Objective: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide. This study aimed to analyze the prognostic value of microRNA-1266-5p (miR-1266-5p) in HCC patients and investigate its biological function in HCC progression.

Methods: The expression of miR-1266-5p in tissues and cells was measured by quantitative real-time PCR (qRT-PCR). Read More

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Exploring the Role and Mechanism of pAMPK-Mediated Dysregulation of Brf1 and RNA Pol III Genes.

Oxid Med Cell Longev 2021 20;2021:5554932. Epub 2021 Apr 20.

Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.

TF IIB-related factor 1 (Brf1) is a key transcription factor of RNA polymerase III (Pol III) genes. Our early studies have demonstrated that Brf1 and Pol III genes are epigenetically modulated by histone H3 phosphorylation. Here, we have further investigated the relationship of the abnormal expression of Brf1 with a high level of phosphorylated AMPK (pAMPK) and explored the role and molecular mechanism of pAMPK-mediated dysregulation of Brf1 and Pol III genes in lung cancer. Read More

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Eukaryotic RNA Polymerases: The Many Ways to Transcribe a Gene.

Front Mol Biosci 2021 21;8:663209. Epub 2021 Apr 21.

Instituto de Biotecnología y Biomedicina (Biotecmed), Universitat de València, València, Spain.

In eukaryotic cells, three nuclear RNA polymerases (RNA pols) carry out the transcription from DNA to RNA, and they all seem to have evolved from a single enzyme present in the common ancestor with archaea. The multiplicity of eukaryotic RNA pols allows each one to remain specialized in the synthesis of a subset of transcripts, which are different in the function, length, cell abundance, diversity, and promoter organization of the corresponding genes. We hypothesize that this specialization of RNA pols has conditioned the evolution of the regulatory mechanisms used to transcribe each gene subset to cope with environmental changes. Read More

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Evaluation of a novel particle-based multi-analyte technology for the detection of anti-fibrillarin antibodies.

Immunol Res 2021 Apr 28. Epub 2021 Apr 28.

Immunologia E Allergologia, Ospedale S. Maria degli Angeli, Pordenone, Italy.

Systemic sclerosis (SSc) is a heterogeneous autoimmune disease associated with several anti-nuclear antibodies (ANA), including those in the classification criteria (anti-centromere, anti-topoisomerase I (Scl-70), anti-RNA Pol III). However, the presence of less common antibodies such as anti-fibrillarin (U3-RNP) that generate a clumpy nucleolar pattern by HEp-2 indirect immunofluorescence assay (IFA, ICAP AC-9) are considered disease specific and are with clinical subsets of SSc, therefore playing a role in diagnosis and prognosis. A specific and sensitive anti-fibrillarin assay would be an important addition to serological diagnosis and evaluation of SSc. Read More

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Lytic infection with murine gammaherpesvirus 68 activates host and viral RNA polymerase III promoters and enhances non-coding RNA expression.

J Virol 2021 Apr 28. Epub 2021 Apr 28.

Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.

RNA polymerase III (pol III) transcribes multiple non-coding (nc) RNAs that are essential for cellular function. Pol III-dependent transcription is also engaged during certain viral infections, including the gammaherpesviruses (γHVs), where pol III-dependent viral ncRNAs promote pathogenesis. Additionally, several host ncRNAs are upregulated during γHV infection and play integral roles in pathogenesis by facilitating viral establishment and gene expression. Read More

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Associations between constipation symptoms and the sleep quality in non-dialysis chronic kidney disease patients: a cross-sectional study.

Pol Arch Intern Med 2021 Apr 27. Epub 2021 Apr 27.

Introduction: Sleep disturbances, similarly to constipation-related symptoms, are common problems in chronic kidney disease (CKD) patients and are associated with worse health-related quality of life.

Objectives: We aimed at detailing the sleep problems in conservatively-treated CKD patients, and at verifying the associations between the sleep quality and constipation in these patients.

Patients And Methods: In this cross-sectional study, 100 conservatively-treated CKD outpatients filled questionnaires that included questions addressing sleep quality (The Medical Outcomes Study 12-item Sleep Scale-Revised (MOS-Sleep-R)) and constipation-related symptoms (PAC-SYM, Rome III criteria). Read More

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gEL DNA: A Cloning- and Polymerase Chain Reaction-Free Method for CRISPR-Based Multiplexed Genome Editing.

CRISPR J 2021 Apr 23. Epub 2021 Apr 23.

Department of Biotechnology, Delft University of Biotechnology, Delft, Netherlands.

Even for the genetically accessible yeast , the CRISPR-Cas DNA editing technology has strongly accelerated and facilitated strain construction. Several methods have been validated for fast and highly efficient single editing events, and diverse approaches for multiplex genome editing have been described in the literature by means of Cas9 or Cas12a endonucleases and their associated guide RNAs (gRNAs). The gRNAs used to guide the Cas endonuclease to the editing site are typically expressed from plasmids using native Pol II or Pol III RNA polymerases. Read More

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Platelet function in patients undergoing surgical and transcatheter aortic valve replacement: a comparative study.

Kardiol Pol 2021 Apr 20. Epub 2021 Apr 20.

Background: Intervention-induced platelet hypercoagulability may pose a risk of serious adverse events for patients.

Aims: The aim of this study was to assess whether surgical (SAVR) and transcatheter aortic valve replacement (TAVR) differ in periprocedural platelet activity.

Methods: The total number of 24 patients with a mean age (SD) of 71 (13) years who underwent SAVR (n=12) or TAVR (n=12) were recruited for the study. Read More

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Potential roles of condensin in genome organization and beyond in fission yeast.

Authors:
Kyoung-Dong Kim

J Microbiol 2021 May 20;59(5):449-459. Epub 2021 Apr 20.

Department of Systems Biotechnology, Chung-Ang University, Anseong, 17546, Republic of Korea.

The genome is highly organized hierarchically by the function of structural maintenance of chromosomes (SMC) complex proteins such as condensin and cohesin from bacteria to humans. Although the roles of SMC complex proteins have been well characterized, their specialized roles in nuclear processes remain unclear. Condensin and cohesin have distinct binding sites and mediate long-range and short-range genomic associations, respectively, to form cell cycle-specific genome organization. Read More

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Fallen dogmas: recent advances in locoregionally advanced melanoma.

Pol Arch Intern Med 2021 05 14;131(5):464-468. Epub 2021 Apr 14.

Department of Surgical Oncology, Oncology Center – Prof. Franciszek Łukaszczyk Memorial Hospital, Bydgoszcz, Poland

Last decade brought new achievements in the melanoma research, which resulted in an important changes in the clinical management of stage III melanoma. The article summarizes recent updates with particular focus on practical aspects. Results from surgical studies, Multicenter Selective Lymphadenectomy Trial II (MSLT-II) and German Dermatologic Cooperative Oncology Group (DeCOG-SLT) proved that surgical dogmatic approach that all sentinel node melanoma metastasis warrants completion lymphadenectomy is no longer valid; omission of completion lymphadenectomy in large proportion of sentinel node positive melanoma patients has no negative impact on survival rates. Read More

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FOXA1 is associated with high tumor grade, myometrial invasion and lymph node invasion in endometrial endometrioid carcinoma.

Ginekol Pol 2021 Apr 12. Epub 2021 Apr 12.

University of Health Sciences, Antalya Education and Research Hospital, Department of Pathology, Antalya, Turkey.

Objectives: FOXA1 expression has been demonstrated in several hormone-dependent cancers. However, data are limited concerning the role of FOXA1 in endometrial cancers. The present study aimed to investigate FOXA1 expression via the microarray technique in benign hyperplasia, endometrial intraepithelial neoplasia, and endometrial endometrioid carcinoma. Read More

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Anterior abdominal fixation - a new option in the surgical treatment of pelvic organ prolapse.

Ginekol Pol 2021 Apr 12. Epub 2021 Apr 12.

Medical University of Wroclaw, Departament of Obstetrics and Gynecology, Wroclaw, Poland.

Objectives: To present anterior abdominal fixation - a new surgical technique for the treatment of pelvic organ prolaps (POP) and to evaluate the results of the treatment of patients with stage III and IV POP operated using this technique.

Material And Methods: Anterior abdominal fixation for treating stage III and IV POP was carried out in 42 women, who were qualified according to the Pelvic Organ Prolapse Quantification System (POP-Q) scale at baseline and after 12 months. The Pelvic Floor Disability Index-20 (PFDI-20), along with its symptom scales, were evaluated. Read More

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Recent advances in the pharmacotherapy of pulmonary hypertension: practical considerations.

Kardiol Pol 2021 04 5;79(4):386-392. Epub 2021 Apr 5.

Cardiology Department, Hospital Universitario 12 de Octubre, Madrid, Spain; Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, Madrid, Spain.

Pulmonary arterial hypertension (PAH) is a rare disease characterized by pulmonary vascular remodeling leading to increased vascular resistance. The increased afterload contributes to the development of right ventricular dysfunction and heart failure, which is the leading cause of death among patients with PAH. The development of specific treatments has markedly improved the prognosis of this population. Read More

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Limited Resection Versus Pancreaticoduodenectomy for Duodenal Gastrointestinal Stromal Tumors? Enucleation Interferes in the Debate: A European Multicenter Retrospective Cohort Study.

Ann Surg Oncol 2021 Apr 10. Epub 2021 Apr 10.

Department of Digestive and Oncological Surgery, University Lille, Claude Huriez University Hospital, CHU de Lille, Lille, France.

Background: The optimal surgical procedure for duodenal gastrointestinal stromal tumors (D-GISTs) remains poorly defined. Pancreaticoduodenectomy (PD) allows for a wide resection but is associated with a high morbidity rate.

Objectives: The aim of this study was to compare the short- and long-term outcomes of PD versus limited resection (LR) for D-GISTs and to evaluate the role of tumor enucleation (EN). Read More

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Radiological differences in computed tomography findings and texture analysis between cystic lymph node metastases of human papillomavirus-positive oropharyngeal cancer and second branchial cysts.

Pol J Radiol 2021 25;86:e177-e182. Epub 2021 Mar 25.

Department of Radiology, The Jikei University School of Medicine, Japan.

Purpose: The study aimed to analyse radiological differences in computed tomography (CT) findings and texture analysis between cystic lymph node metastases (CNM) in human papillomavirus (HPV)-positive oropharyngeal cancer (OPC) and second branchial cleft cysts (2 BC).

Material And Methods: Patients with pathological evidence of CNM-HPV-OPC and 2 BC, who underwent contrast-enhanced CT, were retrospectively evaluated. The evaluated characteristics include age, sex, and CT findings. Read More

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Use of T-wave duration and Tpeak-Tend interval as new prognostic markers for patients treated with cardiac resynchronization therapy.

Kardiol Pol 2021 Mar 30. Epub 2021 Mar 30.

Background: The use of electrocardiography (ECG) is a practical method to evaluate the response to cardiac resynchronization therapy (CRT) implantation, as it is easily performed and saves time.

Aims: In this study, it was aimed to assess the predictive value of the T-wave duration and Tpeak-Tend (Tp-e) interval following the CRT implantation administered to heart failure patients.

Methods: Sixty-seven patients with left ventricular ejection fraction ≤ 35, New York Heart Association (NYHA) II-III, ambulatory class-IV, normal sinus rhythm, who have complete left bundle branch block on ECG and treated with CRT were included in this study. Read More

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RNA Sequence and Structure Determinants of Pol III Transcriptional Termination in Human Cells.

J Mol Biol 2021 Jun 1;433(13):166978. Epub 2021 Apr 1.

Center for Synthetic Biology, Northwestern University, 2145 Sheridan Rd, Evanston, IL 60208, USA; Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd, Evanston, IL 60208, USA. Electronic address:

The precise mechanism of transcription termination of the eukaryotic RNA polymerase III (Pol III) has been a subject of considerable debate. Although previous studies have clearly shown that multiple uracils at the end of RNA transcripts are required for Pol III termination, the effects of upstream RNA secondary structure in the nascent transcript on transcriptional termination is still unclear. To address this, we developed an in cellulo Pol III transcription termination assay using the recently developed Tornado-Corn RNA aptamer system to create a Pol III-transcribed RNA that produces a detectable fluorescent signal when transcribed in human cells. Read More

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Spt4 Promotes Pol I Processivity and Transcription Elongation.

Genes (Basel) 2021 Mar 12;12(3). Epub 2021 Mar 12.

Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham 720 20th Street South, Birmingham, AL 35294, USA.

RNA polymerases (Pols) I, II, and III collectively synthesize most of the RNA in a eukaryotic cell. Transcription by Pols I, II, and III is regulated by hundreds of trans-acting factors. One such protein, Spt4, has been previously identified as a transcription factor that influences both Pols I and II. Read More

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Two efficient CRISPR/Cas9 systems for gene editing in soybean.

Transgenic Res 2021 Jun 2;30(3):239-249. Epub 2021 Apr 2.

Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, PqEB, Av W5 Norte Final 716, Brasília, DF, 70770-917, Brazil.

Genome editing using CRISPR/Cas9 has been highlighted as a powerful tool for crop improvement. Nevertheless, its efficiency can be improved, especially for crops with a complex genome, such as soybean. In this work, using the CRISPR/Cas9 technology we evaluated two CRISPR systems, a one-component vs. Read More

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Infective Endocarditis in Patients on Chronic Hemodialysis.

J Am Coll Cardiol 2021 Apr;77(13):1629-1640

Infectious Diseases Service, Hospital Clínic-August Pi i Sunyer Biomedical Research Institute, University of Barcelona, Barcelona, Spain. Electronic address:

Background: Infective endocarditis (IE) is a common and serious complication in patients receiving chronic hemodialysis (HD).

Objectives: This study sought to investigate whether there are significant differences in complications, cardiac surgery, relapses, and mortality between IE cases in HD and non-HD patients.

Methods: Prospective cohort study (International Collaboration on Endocarditis databases, encompassing 7,715 IE episodes from 2000 to 2006 and from 2008 to 2012). Read More

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Standardized procedure to measure the size distribution of extracellular vesicles together with other particles in biofluids with microfluidic resistive pulse sensing.

PLoS One 2021 1;16(4):e0249603. Epub 2021 Apr 1.

Department of Clinical Chemistry, University of Amsterdam location AMC, Amsterdam, The Netherlands.

The particle size distribution (PSD) of extracellular vesicles (EVs) and other submicron particles in biofluids is commonly measured by nanoparticle tracking analysis (NTA) and tunable resistive pulse sensing (TRPS). A new technique for measuring the PSD is microfluidic resistive pulse sensing (MRPS). Because specific guidelines for measuring EVs together with other particles in biofluids with MRPS are lacking, we developed an operating procedure to reproducibly measure the PSD. Read More

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