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Nanomechanics of G-quadruplexes within the promoter of the KIT oncogene.

Nucleic Acids Res 2021 Apr 13. Epub 2021 Apr 13.

School of Medicine and Surgery, BioNanoMedicine Center NANOMIB, University of Milano-Bicocca, 20854 Vedano al Lambro (MB), Italy.

G-quadruplexes (G4s) are tetrahelical DNA structures stabilized by four guanines paired via Hoogsteen hydrogen bonds into quartets. While their presence within eukaryotic DNA is known to play a key role in regulatory processes, their functional mechanisms are still under investigation. In the present work, we analysed the nanomechanical properties of three G4s present within the promoter of the KIT proto-oncogene from a single-molecule point of view through the use of magnetic tweezers (MTs). Read More

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Rule discovery to identify patterns contributing to overrepresentation and severity of run-off-the-road crashes.

Accid Anal Prev 2021 Apr 10;155:106119. Epub 2021 Apr 10.

University of Naples Federico II, Department of Civil, Architectural and Environmental Engineering, Via Claudio 21, 80125, Naples, Italy. Electronic address:

The main objective of this paper was to analyse the roadway, environmental, and driver-related factors associated with an overrepresentation of frequency and severity of run-off-the-road (ROR) crashes. The data used in this study refer to the 6167 crashes occurred in the section Naples-Candela of A16 motorway, Italy in the period from 2001 to 2011. The analysis was carried out using the rule discovery technique due to its ability of extracting knowledge from large amounts of data previously unknown and indistinguishable by investigating patterns that occur together in a given event. Read More

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Hyperspectral Texture Metrology Based on Joint Probability of Spectral and Spatial Distribution.

IEEE Trans Image Process 2021 Apr 13;PP. Epub 2021 Apr 13.

Texture characterization from the metrological point of view is addressed in order to establish a physically relevant and directly interpretable feature. In this regard, a generic formulation is proposed to simultaneously capture the spectral and spatial complexity in hyperspectral images. The feature, named relative spectral difference occurrence matrix (RSDOM) is thus constructed in a multireference, multidirectional, and multiscale context. Read More

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[Clinical-epidemiological characterization of patients with tuberculosis in the Department of Caaguazú, Paraguay. 2014-2017].

Rev Chilena Infectol 2020 Dec;37(6):750-755

Ministerio de Salud Pública y Bienestar Social, Asunción, Paraguay.

Background: Tuberculosis (TB) is one of the ten leading causes of death worldwide.

Aim: To characterize the clinical and epidemiological point of view of TB cases reported in the Department of Caaguazú-Paraguay, from 2014 to 2017.

Methods: Observational, descriptive, retrospective study; Population: 659 cases of TB registered in the National Tuberculosis Control Program (NTCP); variables: age, sex, population group, type of TB, TB/HIV coinfection. Read More

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December 2020

[Molecular detection of gastrointestinal pathogens among children under 5 years old with diarrhea in a hospital center for rotavirus sentinel surveillance in Chile].

Rev Chilena Infectol 2021 Feb;38(1):54-60

Laboratorio de Biología Molecular, Hospital Dr. Luis Calvo Mackenna, Chile.

Background: Infectious diarrhea is still a major problem in public health, especially in children under 5 years of age. The identification of the etiologic agent is important for the clinical management of the diarrhea episode and, from the epidemiological point of view, to implement control measures.

Aim: To determine the presence of gastrointestinal pathogens in children under five years of age with diarrhea in a Chilean rotavirus surveillance center. Read More

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February 2021

Physiological and inflammatory consequences of high and low respiratory rate in acute respiratory distress syndrome.

Acta Anaesthesiol Scand 2021 Apr 12. Epub 2021 Apr 12.

Departamento de Medicina Intensiva, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.

Using protective mechanical ventilation strategies with low tidal volume is usually accompanied by an increment of respiratory rate to maintain adequate alveolar ventilation. However, there is no robust data that support the safety of a high respiratory rate concerning ventilator-induced lung injury. Several experimental animal studies have explored the effects of respiratory rate over lung physiology, using a wide range of frequencies and different models. Read More

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Should Negative Pressure Therapy Replace Any Other Temporary Abdominal Closure Device in Open-Abdomen Management of Secondary Peritonitis?

Surg Technol Int 2021 Apr 9;38. Epub 2021 Apr 9.

Division for Endocrine and Minimally Invasive Surgery of the University of Messina, Italy.

Aim: To clarify the advantages of negative pressure therapy (NPT) compared to other methods of temporary abdominal closure (TAC) in the management of secondary peritonitis.

Methods: We retraced the history of known methods of TAC, and analyzed their advantages and disadvantages. We evaluated as the NPT mechanisms, both from the macroscopic that bio-molecular point of view, well suits to manage this difficult condition. Read More

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[Quality of primary care and quality of life from the point of view of older patients with dizziness. Results oft the cohort study MobilE-TRA].

MMW Fortschr Med 2021 Apr;163(Suppl 4):11-18

der LMU München, Institut für Medizinische Informationsverarbeitung, Biometrie und Epidemiologie (IBE), München, Deutschland.

Background: The treatment of elderly patients with dizziness/vertigo/balance disorders (VDB) can be challenging for their general practitioner. Patient-centered care with a focus on self-management support could be a possible approach. Primary objective of this study was to investigate the correlations between quality of primary care and health-related quality of life (HRQOL) from the patient's perspective. Read More

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How to Handle Co-authorship When Not Everyone's Research Contributions Make It into the Paper.

Sci Eng Ethics 2021 Apr 12;27(2):27. Epub 2021 Apr 12.

Department of Philosophy, Stockholm University, Stockholm, Sweden.

While much of the scholarly work on ethics relating to academic authorship examines the fair distribution of authorship credit, none has yet examined situations where a researcher contributes significantly to the project, but whose contributions do not make it into the final manuscript. Such a scenario is commonplace in collaborative research settings in many disciplines and may occur for a number of reasons, such as excluding research in order to provide the paper with a clearer focus, tell a particular story, or exclude negative results that do not fit the hypothesis. Our concern in this paper is less about the reasons for including or excluding data from a paper and more about distributing credit in this type of scenario. Read More

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First mitotic spindle formation is led by sperm centrosome-dependent MTOCs in humans.

Reproduction 2021 May;161(5):V19-V22

Reproductive Medicine Research Center, Yamashita Shonan Yume Clinic, Fujisawa, Kanagawa, Japan.

Unlike in mice, multinucleated blastomeres appear at a high frequency in the two-cell-stage embryos in humans. In this Point of View article, we demonstrate that the first mitotic spindle formation led by sperm centrosome-dependent microtubule organizing centers may cause a high incidence of zygotic division errors using human tripronuclear zygotes. Read More

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Visual cortical prosthesis: an electrical perspective.

J Med Eng Technol 2021 Apr 12:1-14. Epub 2021 Apr 12.

Department of Integrative and Computational Neuroscience, Paris-Saclay Institute of Neuroscience, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.

The electrical stimulation of the visual cortices has the potential to restore vision to blind individuals. Until now, the results of visual cortical prosthetics have been limited as no prosthesis has restored a full working vision but the field has shown a renewed interest these last years, thanks to wireless and technological advances. However, several scientific and technical challenges are still open to achieve the therapeutic benefit expected by these new devices. Read More

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GPR120/FFAR4 Pharmacology: Focus on Agonists in Type 2 Diabetes Mellitus Drug Discovery.

J Med Chem 2021 Apr 10. Epub 2021 Apr 10.

Department of Biotechnology, Chemistry, and Pharmacy, DoE 2018-2022, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy.

The G-protein coupled receptors (GPCRs) activated by free fatty acids (FFAs) have emerged as new and exciting drug targets, due to their plausible translation from pharmacology to medicines. This perspective aims to report recent research about GPR120/FFAR4 and its involvement in several diseases, including cancer, inflammatory conditions, and central nervous system disorders. The focus is to highlight the importance of GPR120 in Type 2 diabetes mellitus (T2DM). Read More

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Conserved quantities, optimal system and explicit solutions of a (1 + 1)-dimensional generalised coupled mKdV-type system.

J Adv Res 2021 Mar 26;29:159-166. Epub 2020 Oct 26.

International Institute for Symmetry Analysis and Mathematical Modelling & Focus Area for Pure and Applied Analytics, Department of Mathematical Sciences, North-West University, Mafikeng Campus, Private Bag X 2046, Mmabatho 2735, South Africa.

Introduction: The purpose of this paper is to study, a (1 + 1)-dimensional generalised coupled modified Korteweg-de Vries-type system from Lie group analysis point of view. This system is studied in the literature for the first time. The authors found this system to be interesting since it is non-decouplable and possesses higher generalised symmetries. Read More

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Endocrine Therapy-related Endocrinopathies-Biology, Prevalence and Implications for the Management of Breast Cancer.

Oncol Hematol Rev 2020 16;16(1):17-22. Epub 2020 Feb 16.

Department of Medicine, Weill Cornell Medicine, New York, NY, USA.

Nearly 270,000 new breast cancer cases are predicted to be diagnosed in the USA in 2019 with more than 70% being estrogen receptor positive and treated using endocrine therapy. The suppression of estrogen biosynthesis or action via the use of ovarian suppression, aromatase inhibitors and selective estrogen receptor modulators/degraders, respectively, is effective in approximately 70% of women. The systemic inhibition of estrogen during breast cancer treatment is also associated with side effects due to the important endocrine functions of this steroid hormone, including its role in the maintenance of energy homeostasis and bone health. Read More

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February 2020

A Review Article on Wireless Sensor Networks in View of E-epidemic Models.

Wirel Pers Commun 2021 Apr 5:1-17. Epub 2021 Apr 5.

Department of Electronics and Communication Engineering, S. A. Engineering College, Chennai, Tamilnadu India.

Epidemic simulations have recently been used to model the dynamics of malicious codes in the network of wireless sensors. This is because of its open existence, which offers a simple target for malware attacks aimed at disrupting network operations or, worse, causing complete network failure. The Susceptible-Exposed-Infectious-Quarantined-Recovered-Susceptible with Vaccination compartments models like SIR-M, SEIRV, SEIQRV, SEIRS, SITR, SIR with delay are studied by various authors and some of such models that characterize worm dynamics in WSN. Read More

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Piezoelectric tools versus traditional oscillating saw for distal linear osteotomy in hallux valgus correction: Triple-blinded, randomized controlled study.

Foot Ankle Surg 2021 Apr 1. Epub 2021 Apr 1.

II Clinic of Orthopaedics and Traumatology, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy. Electronic address:

Background: The main goal of the surgical correction of the hallux valgus is the morphological correction associated with the functional rebalancing of the first ray. The aim of this triple-blinded, randomized controlled study was to show the efficacy of piezosurgery in performing distal linear osteotomy of the first metatarsal bone in HV correction, in terms of clinical and radiological outcomes at 1-year final follow up.

Methods And Materials: This study was performed collecting prospectively pre-operative and post-operative data for all patients. Read More

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A neural noise account of Gilles de la Tourette syndrome.

Neuroimage Clin 2021 Mar 29;30:102654. Epub 2021 Mar 29.

Cognitive Neurophysiology, Department of Child and Adolescent Psychiatry, Faculty of Medicine, TU, Dresden, Germany; Cognitive Psychology, Faculty of Psychology, Shandong Normal University, Jinan, China.

Tics, often preceded by premonitory urges, are the clinical hallmark of Tourette syndrome. They resemble spontaneous movements, but are exaggerated, repetitive and appear misplaced in a given communication context. Given that tics often go unnoticed, it has been suggested that they represent a surplus of action, or motor noise. Read More

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A roadmap for a Plastisphere.

Mar Pollut Bull 2021 Apr 8;167:112322. Epub 2021 Apr 8.

University of Warsaw, Faculty of Chemistry, Laboratory of Spectroscopy and Intermolecular Interactions, Pasteura 1, 02-093 Warsaw, Poland; University of Warsaw Biological and Chemical Research Centre, Żwirki i Wigury 101 st., 02-089 Warsaw, Poland. Electronic address:

The constantly growing production of synthetic materials and their presence in the environment gradually transform our Blue Planet into the Plastic One. Microplastics (MPs) enlarge significantly their surface during fragmentation processes. Undoubtedly, nanoplastics (NPs), emerging contaminants, and the Plastisphere, the total available surface of debris, are currently on the edge of science. Read More

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Biomimetic Clotrimazole-Loaded PLGA Films with Enhanced Adhesiveness for Controlled Drug Release.

Int J Pharm 2021 Apr 8:120578. Epub 2021 Apr 8.

Department of Pharmaceutical Engineering, Azrieli College of Engineering, Jerusalem, Jerusalem 9103501, Israel. Electronic address:

Biomimetic adhesive surfaces have a number of potential applications in the pharmaceutical and biomedical fields. Fabrication techniques must be adapted to biocompatible and biodegradable materials required for controlled drug release applications. In this study biomimetic adhesive poly(lactic-co-glycolic acid) (PLGA) films loaded with different concentrations of clotrimazole (CTZ) were prepared without combining other adhesive excipients as a controlled release system for potential local oral drug delivery. Read More

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Laccase immobilization onto natural polysaccharides for biosensing and biodegradation.

Carbohydr Polym 2021 Jun 19;262:117963. Epub 2021 Mar 19.

Department of Chemical Engineering, University of New Brunswick, Fredericton, NB, E3B 5 A3, Canada.

Over the last few years, the focus of researchers have been set on enzyme engineering and enzyme immobilization technology using natural polysaccharides as promising and green supporting materials to address the challenges of free enzymes for various applications. Polysaccharides have been extensively implemented as enzyme carriers because they can be easily modified chemically according to the nature of immobilization. This process improves the stability and lifetime of laccase in catalytic reactions. Read More

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Creating a Tool to Assess Interpretive Services Perceptions at a Hospital System Level.

J Immigr Minor Health 2021 Apr 10. Epub 2021 Apr 10.

Department of Pediatrics, The University of Oklahoma Health Sciences Center, 1200 Children's Ave, Ste 14100, Oklahoma City, OK, 73104, USA.

The importance of using professional interpretive services (IS) when communicating with patients and families with limited English proficiency (LEP) is well described in the literature. There is no standardized tool to reliably evaluate IS from the point of view of bedside care providers. Collected local qualitative data and literature review informed an anonymous electronic survey that was distributed to physicians and nursing staff at a tertiary care children's hospital. Read More

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Feasibility, Contrast Sensitivity and Network Specificity of Language fMRI in Presurgical Evaluation for Epilepsy and Brain Tumor Surgery.

Brain Topogr 2021 Apr 10. Epub 2021 Apr 10.

Department of Medicine, School of Health Sciences, Democritus University of Thrace, Xanthi, Greece.

Language fMRI has become an integral part of the planning process in brain surgery. However, fMRI may suffer from confounding factors both on the patient side, as well as on the provider side. In this study, we investigate how patient-related confounds affect the ability of the patient to perform language fMRI tasks (feasibility), the task sensitivity from an image contrast point of view, and the anatomical specificity of expressive and receptive language fMRI protocols. Read More

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Dynamic image quantization using Leaky Integrate-and-Fire neurons.

IEEE Trans Image Process 2021 Apr 9;PP. Epub 2021 Apr 9.

This paper introduces a novel coding/decoding mechanism that mimics one of the most important properties of the human visual system: its ability to enhance the visual perception quality in time. In other words, the brain takes advantage of time to process and clarify the details of the visual scene. This characteristic is yet to be considered by the state-of-the-art quantization mechanisms that process the visual information regardless the duration of time it appears in the visual scene. Read More

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Echo-SyncNet: Self-supervised Cardiac View Synchronization in Echocardiography.

IEEE Trans Med Imaging 2021 Apr 9;PP. Epub 2021 Apr 9.

In echocardiography (echo), an electrocardiogram (ECG) is conventionally used to temporally align different cardiac views for assessing critical measurements. However, in emergencies or point-of-care situations, acquiring an ECG is often not an option, hence motivating the need for alternative temporal synchronization methods. Here, we propose Echo-SyncNet, a self-supervised learning framework to synchronize various cross-sectional 2D echo series without any human supervision or external inputs. Read More

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Nanopore RNA Sequencing Analysis.

Methods Mol Biol 2021 ;2284:569-578

European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.

The recent advent of Nanopore sequencing allows for the sequencing of full-length RNA or cDNA molecules. This new type of data introduces new challenges from the computational point of view, and requires new software as well as dedicated analysis pipelines. In this chapter, we guide the reader through the typical analysis steps required to process the raw data produced by the instrument into a table of counts suitable for downstream analyses. Read More

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January 2021

Innovative approaches to designing and manufacturing a prosthetic thumb.

Prosthet Orthot Int 2021 Feb;45(1):81-84

Department of Biomedical Engineering and Measurement, Faculty of Mechanical Engineering, Technical University of Košice, Košice, Slovakia.

Case Description: Conventional methods for producing custom prosthetic fingers are time-consuming, can be uncomfortable for the patient, and require a skilled prosthetist. The subject was a 40-year-old male with congenital absence of the thumb and related metacarpal bone on the right non-dominant hand, anomaly of the lengths of individual upper limb segments, and contracture of the elbow joint. This hand presentation made it impossible for him to perform thumb opposition, which is a very important function for common daily activities. Read More

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February 2021

Dental Pulp Stem Cells on Implant Surface: An In Vitro Study.

Biomed Res Int 2021 23;2021:3582342. Epub 2021 Mar 23.

Department of Biomedical and Dental Sciences and Morphological and Functional Imaging, Messina University, Messina 98100, Italy.

In the field of biology and medicine, one hears often about stem cells and their potential. The dental implant new surfaces, subjected to specific treatments, perform better and allow for quicker healing times and better clinical performance. The purpose of this study is to evaluate from a biological point of view the interaction and cytotoxicity between stem cells derived from dental pulp (DPSCs) and titanium surfaces. Read More

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Uncovering salt tolerance mechanisms in pepper plants: a physiological and transcriptomic approach.

BMC Plant Biol 2021 Apr 8;21(1):169. Epub 2021 Apr 8.

Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-C.S.I.C, Camino de Vera s/n, 46022, Valencia, Spain.

Background: Pepper is one of the most cultivated crops worldwide, but is sensitive to salinity. This sensitivity is dependent on varieties and our knowledge about how they can face such stress is limited, mainly according to a molecular point of view. This is the main reason why we decided to develop this transcriptomic analysis. Read More

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Changes in geometrical aspects of a simple model of cilia synchronization control the dynamical state, a possible mechanism for switching of swimming gaits in microswimmers.

PLoS One 2021 8;16(4):e0249060. Epub 2021 Apr 8.

Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom.

Active oscillators, with purely hydrodynamic coupling, are useful simple models to understand various aspects of motile cilia synchronization. Motile cilia are used by microorganisms to swim and to control the flow fields in their surroundings; the patterns observed in cilia carpets can be remarkably complex, and can be changed over time by the organism. It is often not known to what extent the coupling between cilia is due to just hydrodynamic forces, and neither is it known if it is biological or physical triggers that can change the dynamical collective state. Read More

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Petersen Graph Multi-Orientation based Multi-Scale Ternary Pattern (PGMO-MSTP): An Efficient Descriptor for Texture and Material Recognition.

IEEE Trans Image Process 2021 Apr 8;PP. Epub 2021 Apr 8.

Classifying and modeling texture images, especially those with significant rotation, illumination, scale, and view-point variations, is a hot topic in the computer vision field. Inspired by local graph structure (LGS), local ternary patterns (LTP), and their variants, this paper proposes a novel image feature descriptor for texture and material classification, which we call Petersen Graph Multi-Orientation based Multi-Scale Ternary Pattern (PGMO-MSTP). PGMO-MSTP is a histogram representation that efficiently encodes the joint information within an image across feature and scale spaces, exploiting the concepts of both LTP-like and LGS-like descriptors, in order to overcome the shortcomings of these approaches. Read More

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