80 results match your criteria pmta

The Phospholipid -Methyltransferase and Phosphatidylcholine Synthase Pathways and the ChoXWV Choline Uptake System Involved in Phosphatidylcholine Synthesis Are Widely Conserved in Most, but Not All Species.

Front Microbiol 2021 4;12:614243. Epub 2021 Aug 4.

Dpto. de Microbiología y Parasitología, Instituto de Salud Tropical (ISTUN), Instituto de Investigación Sanitaria de Navarra, Universidad de Navarra, Pamplona, Spain.

The brucellae are facultative intracellular bacteria with a cell envelope rich in phosphatidylcholine (PC). PC is abundant in eukaryotes but rare in prokaryotes, and it has been proposed that uses PC to mimic eukaryotic-like features and avoid innate immune responses in the host. Two PC synthesis pathways are known in prokaryotes: the PmtA-catalyzed trimethylation of phosphatidylethanolamine and the direct linkage of choline to CDP-diacylglycerol catalyzed by the PC synthase Pcs. Read More

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A Markerless Gene Deletion System in by Using the Copper-Inducible YoeB Toxin as a Counterselectable Marker.

Microorganisms 2021 May 19;9(5). Epub 2021 May 19.

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

is an important zoonotic pathogen causing severe infections in swine and humans. Induction of the YoeB toxin in resulted in cell death, leading to the speculation that YoeB can be a counterselectable marker. Herein, the counterselection potential of YoeB was assessed in . Read More

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Effect of exposure to root canal irrigants on the push-out bond strength of calcium silicate-based cements.

Clin Oral Investig 2021 May 28;25(5):3267-3274. Epub 2020 Oct 28.

Departamento de Odontología Conservadora y Prótesis Bucofacial, Facultad de Odontología, Universidad Complutense de Madrid, Pza. Ramón y Cajal, s/n. Ciudad Universitaria, 28040, Madrid, Spain.

Objectives: The aim of this study was to compare the push-out bond strength (POBS) of three calcium silicate-based cements (CSBC) after exposure to saline, sodium hypochlorite (NaOCl), ethylenediaminetetraacetic acid (EDTA), and a mixture of NaOCl and etidronic acid (NaOCl+HEBP) in simulated perforation.

Materials And Methods: A total of 180 dentin slices were perforated and then filled with either Biodentine (BD), ProRoot MTA (PMTA), or Total Fill (TF). After 1 week, specimens in each group were divided into 4 subgroups (n = 15) according to the root canal irrigant (RCI) that their coronal surface was exposed to: saline, 5. Read More

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Postendodontic Pain after Pulpotomy or Root Canal Treatment in Mature Teeth with Carious Pulp Exposure: A Multicenter Randomized Controlled Trial.

Pain Res Manag 2020 30;2020:5853412. Epub 2020 Jun 30.

Endodontology Research Center, Endodontic Department, Dental School, Kerman University of Medical Sciences, Kerman 7618751689, Iran.

This equivalence, randomized, clinical trial aimed to compare the postoperative pain of root canal therapy (RCT) with pulpotomy with mineral trioxide aggregate (PMTA) or calcium-enriched mixture (PCEM) in permanent mature teeth. In seven academic centers, 550 cariously exposed pulps were included and randomly allocated into PMTA ( = 188), PCEM ( = 194), or RCT ( = 168) arms. Preoperative "Pain Intensity" (PI) on Numerical Rating Scale and postoperative PIs until day 7 were recorded. Read More

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November 2020

Papillary muscles. Dark side of the heart: A simple approach for a forgotten structure.

Echocardiography 2020 07 30;37(7):993-998. Epub 2020 Jun 30.

Department of Cardiology, ICSI Sanatorio Las Lomas, San Isidro, Argentina.

Purpose: Transthoracic echocardiography (TTE) is currently the election method for initial screening of left ventricular papillary muscles (PM). However, diagnosis of borderline PM hypertrophy with TTE is not always feasible due to cumbersome interpretation of different PM diameters and the absence of precise normal ranges in the literature. The objective of this study was to obtain TTE normal cutoff values and to describe convenient indexes of PM dimensions. Read More

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Analysis of FDA's IQOS marketing authorisation and its policy impacts.

Tob Control 2020 Jun 29. Epub 2020 Jun 29.

Center for Tobacco Control Research & Education, University of California, San Francisco, California, USA

Background: Philip Morris Products SA (PMPSA) submitted a premarket tobacco application (PMTA) to US Food and Drug Administration (FDA) seeking an order permitting it to market IQOS in the USA. US law requires FDA to deny marketing authorisation if applicants fail to demonstrate that their product is 'appropriate for the protection of the public health'. FDA issued a marketing order for IQOS in April 2019, which Philip Morris is using to promote IQOS outside the USA. Read More

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Genetic diversity of Legionella pcs and pmtA genes and the effect of utilization of choline by Legionella spp. on induction of proinflammatory cytokines.

Pathog Dis 2019 10;77(7)

Department of Biophysics, Faculty of Mathematics, Physics and Computer Science, Maria Curie-Sklodowska University, Maria Curie-Sklodowska Square 1, 20-031 Lublin, Lublin, Poland.

Legionella species synthesize phosphatidylcholine (PC) in two independent pathways: the three-step methylation of phosphatidylethanolamine PMT pathway and the one-step PCS pathway, in which the Pcs enzyme catalyzes the reaction between choline and CDP-diacylglycerol to form PC. Legionella pcs genes encode highly hydrophobic proteins with phosphatidylcholine synthase activity, which contain up to eight transmembrane helices with N- and C-termini located inside the bacterial cell. The comparative analysis of nucleotide sequences of pcs showed that these genes share high sequence identity among members of the Legionellaceae family. Read More

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October 2019

PmtA functions as a ferrous iron and cobalt efflux pump in .

Emerg Microbes Infect 2019 ;8(1):1254-1264

Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University , Yangzhou , People's Republic of China.

Transition metals are nutrients essential for life. However, an excess of metals can be toxic to cells, and host-imposed metal toxicity is an important mechanism for controlling bacterial infection. Accordingly, bacteria have evolved metal efflux systems to maintain metal homeostasis. Read More

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January 2020

Perioperative changes of the slope in the preload recruitable stroke work relationship by a single-beat technique after mitral valve surgery in functional mitral regurgitation with non-ischemic dilated cardiomyopathy.

Gen Thorac Cardiovasc Surg 2020 Jan 22;68(1):30-37. Epub 2019 Jun 22.

Department of Cardiovascular and Thoracic Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, 060-8638, Japan.

Objectives: The slope in the preload recruitable stroke work relationship is a highly linear, load-insensitive contractile parameter. However, the perioperative change of the slope has not been reported before. We examined the perioperative slope from a steady-state single beat in patients with functional mitral regurgitation and assessed the correlation with brain natriuretic peptide (BNP) levels. Read More

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January 2020

An ion for an iron: streptococcal metal homeostasis under oxidative stress.

Biochem J 2019 02 28;476(4):699-703. Epub 2019 Feb 28.

School of Dental Sciences, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4BW, U.K.

The ability of opportunistic pathogens such as Group A (GAS) to transition between mucosal colonisation and invasive disease requires complex systems for adapting to markedly different host environments. The battle to acquire essential trace metals such as manganese and iron from the host is central to pathogenesis. Using a molecular genetic approach, Turner et al. Read More

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February 2019

Group A co-ordinates manganese import and iron efflux in response to hydrogen peroxide stress.

Biochem J 2019 02 14;476(3):595-611. Epub 2019 Feb 14.

School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia 4072, Australia

Bacterial pathogens encounter a variety of adverse physiological conditions during infection, including metal starvation, metal overload and oxidative stress. Here, we demonstrate that group A (GAS) utilises Mn(II) import via MtsABC during conditions of hydrogen peroxide stress to optimally metallate the superoxide dismutase, SodA, with Mn. MtsABC expression is controlled by the DtxR family metalloregulator MtsR, which also regulates the expression of Fe uptake systems in GAS. Read More

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February 2019

Effect of ProRoot MTA® and Biodentine® on osteoclastic differentiation and activity of mouse bone marrow macrophages.

J Appl Oral Sci 2019 Jan 7;27:e20180150. Epub 2019 Jan 7.

Dankook University, College of Dentistry, Department of Conservative Dentistry, Cheonan, Korea.

Objectives: This investigation aimed to assess the differentiation inhibitory effects of ProRoot MTA® (PMTA) and Biodentine® (BIOD) on osteoclasts originated from murine bone marrow macrophages (BMMs) and compare these effects with those of alendronate (ALD).

Materials And Methods: Mouse BMMs were cultured to differentiate into osteoclasts with macrophage colony-stimulating factor and receptor activator of NF-κB (RANKL), treated with lipopolysaccharide. After application with PMTA, BIOD, or ALD, cell toxicities were examined using WST-1 assay kit, and RANKL-induced osteoclast differentiation and activities were determined by resorption pit formation assay and tartrate-resistant acid phosphate (TRAP) staining. Read More

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January 2019

Facile fabrication of gelatin and polycaprolactone based bilayered membranes via spin coating method with antibacterial and cyto-compatible properties.

Int J Biol Macromol 2019 Mar 28;124:699-707. Epub 2018 Nov 28.

College of Light Industry & Textile & Food Engineering, Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, Sichuan University, Chengdu 610065, China.

A growth of bacterial infections and over-and inefficient release of antibiotics forces one to search new antibacterial agents and/or strategies. In this study, a novel strategy towards biocompatible and antibacterial bilayer wound dressing was proposed by a two-step spin coating method combined with in-situ crosslinking polymerization. First, through in-situ crosslinking polymerization, [2-(methacryloyloxy) ethyl] trimethylammonium chloride ([MTA][Cl]) was polymerized and crosslinked in polycaprolactone (PCL) solution and PCL/PMTA solution was obtained. Read More

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Effects of four novel root-end filling materials on the viability of periodontal ligament fibroblasts.

Restor Dent Endod 2018 Aug 25;43(3):e24. Epub 2018 May 25.

Department of Endodontics, Faculty of Dentistry, Selcuk University, Konya, Turkey.

Objectives: The aim of this study was to evaluate the biocompatibility of newly proposed root-end filling materials, Biodentine, Micro-Mega mineral trioxide aggregate (MM-MTA), polymethylmethacrylate (PMMA) bone cement, and Smart Dentin Replacement (SDR), in comparison with contemporary root-end filling materials, intermediate restorative material (IRM), Dyract compomer, ProRoot MTA (PMTA), and Vitrebond, using human periodontal ligament (hPDL) fibroblasts.

Materials And Methods: Ten discs from each material were fabricated in sterile Teflon molds and 24-hour eluates were obtained from each root-end filling material in cell culture media after 1- or 3-day setting. hPDL fibroblasts were plated at a density of 5 × 10/well, and were incubated for 24 hours with 1:1, 1:2, 1:4, and 1:8 dilutions of eluates. Read More

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[Surgical Options for Refractory Heart Failure Patients with Non-ischemic Dilated Cardiomyopathy].

Kyobu Geka 2018 Jul;71(7):484-487

Department of Cardiovascular and Thoracic Surgery, Hokkaido University, Sapporo, Japan.

Surgical strategy for non-ischemic dilated cardiomyopathy (NIDCM) is currently controversial. Subjects were 20 patients who underwent left ventriculoplasty(LVP) from 2006 to 2013 and 6 patients who underwent papillary muscle tugging approximation (PMTA) after 2015. PMTA is a new trans-mitral approach combined with valve replacement without left ventriculotomy. Read More

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Protein O-mannosyltransferases are required for sterigmatocystin production and developmental processes in Aspergillus nidulans.

Curr Genet 2018 Oct 28;64(5):1043-1056. Epub 2018 Feb 28.

Department of Biological Science and Technology, Tokyo University of Science, Tokyo, Japan.

Aspergillus nidulans produces sterigmatocystin (ST), a precursor of a carcinogenic secondary metabolite aflatoxin (AF), during its developmental process. ST biosynthesis has been shown to be affected by various regulatory factors. In this study, we investigated the involvement of O-mannosyltransferases (PmtA, PmtB, PmtC), in ST production and morphological development. Read More

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October 2018

Whole-genome sequencing enabling the detection of a colistin-resistant hypermutating Citrobacter werkmanii strain harbouring a novel metallo-β-lactamase VIM-48.

Int J Antimicrob Agents 2018 Jun 22;51(6):867-874. Epub 2018 May 22.

Institute of Medical Microbiology and Hygiene, University of Tübingen, Tübingen, Germany; German Center for Infection Research (DZIF), partner site Tübingen, Tübingen, Germany.

Citrobacter spp. harbouring metallo-β-lactamases (MBLs) have been reported from various countries and different sources, but their isolation from clinical specimens remains a rare event in Europe. MBL-harbouring Enterobacteriaceae are considered a major threat in infection control as therapeutic options are often limited to colistin. Read More

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Tooth discoloration effects of calcium silicate based barrier materials used in revascularization and treatment with internal bleaching.

J Dent Sci 2017 Dec 9;12(4):347-353. Epub 2017 May 9.

Selcuk University, Faculty of Dentistry, Department of Endodontics, Konya, Turkey.

Background/purpose: Usage of barrier materials is an important step in revascularization procedure. One of the undesired properties of these barrier materials is to cause coronal tooth discoloration. The aim of this study was to evaluate the tooth discoloration induced by ProRoot MTA (PMTA), Biodentine, and MM-MTA, as well as the efficacy of internal bleaching on this discoloration. Read More

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December 2017

Does Calipered Kinematically Aligned TKA Restore Native Left to Right Symmetry of the Lower Limb and Improve Function?

J Arthroplasty 2018 02 25;33(2):398-406. Epub 2017 Sep 25.

Department of Mechanical Engineering, University of California, Davis, Davis, California.

Background: Kinematically aligned total knee arthroplasty (KA TKA) strives to restore the native left to right symmetry of the lower limb; however, the reproducibility of achieving this target is unknown. The present study determined the proportion of patients with left to right symmetry and the improvement in patient-reported function after calipered KA TKA.

Methods: A review of 562 postoperative scanograms identified 102 patients (53 women) with a KA TKA in one limb, no other skeletal abnormalities in either limb, and symmetrical rotation between limbs on the scanogram. Read More

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February 2018

Does alignment of the limb and tibial width determine relative narrowing between compartments when planning mechanically aligned TKA?

Arch Orthop Trauma Surg 2018 Jan 25;138(1):91-97. Epub 2017 Oct 25.

Department of Biomedical Engineering, University of California, Davis, Davis, CA, 95616, USA.

Introduction: We determined (1) the range of the hip-knee-ankle (HKA) angle in the native or pre-arthritic limbs of patients with a contralateral total knee arthroplasty (TKA); and when mechanical alignment is planned (2) the relationships between the HKA angle and the tibial width, and the relative narrowing between the medial and lateral compartments and (3) the effect of tibial width on the range of narrowing.

Methods: The HKA angle, distal lateral femoral angle (DLFA), and proximal medial tibial angle (PMTA) were measured on the native limb of 102 subjects (53 female) treated with contralateral TKA. The sine of the angle of the resection gap (PMTA minus 90° subtracted from the DLFA minus 90°) multiplied by the tibial width and by narrow (59 mm), average (75 mm), and wide (91 mm) tibias computed relative narrowing. Read More

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January 2018

Do varus or valgus outliers have higher forces in the medial or lateral compartments than those which are in-range after a kinematically aligned total knee arthroplasty? limb and joint line alignment after kinematically aligned total knee arthroplasty.

Bone Joint J 2017 Oct;99-B(10):1319-1328

University of California, Davis, USA, Davis, California 95616.

Aims: The aims of this study were to determine the proportion of patients with outlier varus or valgus alignment in kinematically aligned total knee arthroplasty (TKA), whether those with outlier varus or valgus alignment have higher forces in the medial or lateral compartments of the knee than those with in-range alignment and whether measurements of the alignment of the limb, knee and components predict compartment forces.

Patients And Methods: The intra-operative forces in the medial and lateral compartments were measured with an instrumented tibial insert in 67 patients who underwent a kinematically aligned TKA during passive movement. The mean of the forces at full extension, 45° and 90° of flexion determined the force in the medial and lateral compartments. Read More

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October 2017

Dissection of membrane-binding and -remodeling regions in two classes of bacterial phospholipid N-methyltransferases.

Biochim Biophys Acta Biomembr 2017 Dec 11;1859(12):2279-2288. Epub 2017 Sep 11.

Microbial Biology, Faculty of Biology, Ruhr University Bochum, Bochum, Germany. Electronic address:

Bacterial phospholipid N-methyltransferases (Pmts) catalyze the formation of phosphatidylcholine (PC) via successive N-methylation of phosphatidylethanolamine (PE). They are classified into Sinorhizobium-type and Rhodobacter-type enzymes. The Sinorhizobium-type PmtA protein from the plant pathogen Agrobacterium tumefaciens is recruited to anionic lipids in the cytoplasmic membrane via two amphipathic helices called αA and αF. Read More

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December 2017

The PerR-Regulated P-Type ATPase (PmtA) Acts as a Ferrous Iron Efflux Pump in Streptococcus pyogenes.

Infect Immun 2017 06 23;85(6). Epub 2017 May 23.

School of Chemistry and Molecular Biosciences and Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD, Australia

(group A [GAS]) is an obligate human pathogen responsible for a broad spectrum of human disease. GAS has a requirement for metal homeostasis within the human host and, as such, tightly modulates metal uptake and efflux during infection. Metal acquisition systems are required to combat metal sequestration by the host, while metal efflux systems are essential to protect against metal overload poisoning. Read More

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Effect of Sodium Hypochlorite on Push-out Bond Strength of Four Calcium Silicate-based Endodontic Materials when used for repairing Perforations on Human Dentin: An in vitro Evaluation.

Sara A Alsubait

J Contemp Dent Pract 2017 Apr 1;18(4):289-294. Epub 2017 Apr 1.

Department of Restorative Dental Science, College of Dentistry King Saud University, Riyadh, Kingdom of Saudi Arabia, e-mail:

Aim: This study aimed to evaluate the push-out bond strength of NeoMTA Plus (NMTA), EndoSequence root repair material fast set putty (ERRMF), biodentine (BD), and ProRoot white mineral trioxide aggregate (PMTA) when used as perforation repair materials after exposure to 2.5% sodium hypochlorite (NaOCl) during the early setting phase.

Materials And Methods: Horizontal midroot sections were prepared from single-rooted human teeth. Read More

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Iron Efflux by PmtA Is Critical for Oxidative Stress Resistance and Contributes Significantly to Group A Streptococcus Virulence.

Infect Immun 2017 06 23;85(6). Epub 2017 May 23.

Center for Molecular and Translational Human Infectious Diseases Research, Houston Methodist Research Institute, and Department of Pathology and Genomic Medicine, Houston Methodist Hospital, Houston, Texas, USA

Group A (GAS) is a human-only pathogen that causes a spectrum of disease conditions. Given its survival in inflamed lesions, the ability to sense and overcome oxidative stress is critical for GAS pathogenesis. PerR senses oxidative stress and coordinates the regulation of genes involved in GAS antioxidant defenses. Read More

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Membrane Remodeling by a Bacterial Phospholipid-Methylating Enzyme.

mBio 2017 02 14;8(1). Epub 2017 Feb 14.

Microbial Biology, Faculty of Biology, Ruhr University Bochum, Bochum, Germany

Membrane deformation by proteins is a universal phenomenon that has been studied extensively in eukaryotes but much less in prokaryotes. In this study, we discovered a membrane-deforming activity of the phospholipid -methyltransferase PmtA from the plant-pathogenic bacterium PmtA catalyzes the successive three-step -methylation of phosphatidylethanolamine to phosphatidylcholine. Here, we defined the lipid and protein requirements for the membrane-remodeling activity of PmtA by a combination of transmission electron microscopy and liposome interaction studies. Read More

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February 2017

The effect of human blood on the setting and surface micro-hardness of calcium silicate cements.

Clin Oral Investig 2016 Nov 23;20(8):1997-2005. Epub 2015 Dec 23.

Microscope Center, Department of Conservative Dentistry and Oral Science Research Center, College of Dentistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, South Korea.

Objectives: The purpose of the present study was to evaluate the effects of human blood on the setting and microhardness of calcium silicate cements.

Materials And Methods: Three types of silicate-based cements were used: ProRoot MTA (PMTA), OrthoMTA (OMTA), and RetroMTA (RMTA). Mixed cement was placed into polyethylene molds with lengths of 2 and 4 mm. Read More

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November 2016

'Effects of novel root repair materials on attachment and morphological behaviour of periodontal ligament fibroblasts: Scanning electron microscopy observation'.

Microsc Res Tech 2016 Dec 20;79(12):1214-1221. Epub 2016 Sep 20.

Department of Endodontics, Faculty of Dentistry, Selcuk University, Konya, Turkey.

The aim of this study was to evaluate the adhesion of periodontal ligament fibroblasts (PDLs) on newly proposed root repair materials [Biodentine, MM-MTA, polymethylmethacrylate (PMMA) bone cement, and SDR], in comparison with contemporary root repair materials [IRM, Dyract compomer, ProRoot MTA (PMTA), and Vitrebond]. Five discs from each material were fabricated in sterile Teflon molds, and the specimens were aged and prewetted in cell culture media for 96 hours. Three material discs were used for scanning electron microscopy (SEM) for the assessment of the attachment, density, and morphological changes in the PDLs, while two samples were used for energy dispersive x-ray spectroscopy (SEM-EDX) to determine the elemental composition of the materials. Read More

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December 2016

A Comparison of the Discoloration Potential for EndoSequence Bioceramic Root Repair Material Fast Set Putty and ProRoot MTA in Human Teeth: An In Vitro Study.

J Esthet Restor Dent 2017 Feb 17;29(1):59-67. Epub 2016 Sep 17.

Dental intern, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.

Objective: To compare the discoloration potential of Endosequence Bioceramic Root Repair Material fast set putty (ERRMF) and ProRoot MTA (PMTA) when placed coronally in human extracted teeth over a 4-month period.

Methodology: Forty-eight premolars were sectioned 2 mm below the cementoenamel junction. The pulp chambers were cleaned chemo-mechanically. Read More

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February 2017