38,680 results match your criteria pluripotent stem


Generation of a human induced pluripotent stem cell line PUMCHi017-A from a Choroideremia patient with CHM mutation.

Stem Cell Res 2022 Jan 10;59:102661. Epub 2022 Jan 10.

Department of Ophthalmology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. Electronic address:

Choroideremia (CHM) is a rare monogenic, X-linked recessive inherited chorioretinal dystrophy caused by loss of function variants in the CHM gene. We successfully generated a novel human induced pluripotent stem cell (hiPSC) line from a CHM patient with CHM variant using the Sendai-virus based approach. These cells will provide a disease model for further studies on the disease pathogenesis and potential interventions. Read More

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January 2022

SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoids.

Cell Stem Cell 2021 Dec 25. Epub 2021 Dec 25.

Institute of Experimental Medicine and Systems Biology, RWTH Aachen University, Aachen, Germany; Division of Nephrology and Clinical Immunology, RWTH Aachen University, Aachen, Germany.

Kidney failure is frequently observed during and after COVID-19, but it remains elusive whether this is a direct effect of the virus. Here, we report that SARS-CoV-2 directly infects kidney cells and is associated with increased tubule-interstitial kidney fibrosis in patient autopsy samples. To study direct effects of the virus on the kidney independent of systemic effects of COVID-19, we infected human-induced pluripotent stem-cell-derived kidney organoids with SARS-CoV-2. Read More

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December 2021

Tissue Culture Models of Acute Kidney Injury: From Tubule Cells to Human Kidney Organoids.

J Am Soc Nephrol 2022 Jan 14. Epub 2022 Jan 14.

L Woodard, Department of Medicine, Division of Nephrology and Hypertension, Vanderbilt University Medical Center, Nashville, United States.

Acute kidney injury (AKI) affects approximately 13.3 million people around the world each year, causing chronic kidney disease and/or mortality. The mammalian kidney cannot generate new nephrons after postnatal renal damage and regenerative therapies for AKI are not available. Read More

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January 2022

Inducing human retinal pigment epithelium-like cells from somatic tissue.

Stem Cell Reports 2022 Jan 3. Epub 2022 Jan 3.

RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan. Electronic address:

Regenerative medicine relies on basic research outcomes that are only practical when cost effective. The human eyeball requires the retinal pigment epithelium (RPE) to interface the neural retina and the choroid at large. Millions of people suffer from age-related macular degeneration (AMD), a blinding multifactor genetic disease among RPE degradation pathologies. Read More

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January 2022

PAX6-positive microglia evolve locally in hiPSC-derived ocular organoids.

Stem Cell Reports 2021 Dec 27. Epub 2021 Dec 27.

Department of Ophthalmology, Osaka University Graduate School of Medicine, Osaka, Japan; Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Osaka, Japan. Electronic address:

Microglia are the resident immune cells of the central nervous system (CNS). They govern the immunogenicity of the retina, which is considered to be part of the CNS; however, it is not known how microglia develop in the eye. Here, we studied human-induced pluripotent stem cells (hiPSCs) that had been expanded into a self-formed ectodermal autonomous multi-zone (SEAM) of cells that partially mimics human eye development. Read More

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December 2021

Comprehensive analyses of the inotropic compound omecamtiv mecarbil in rat and human cardiac preparations.

Am J Physiol Heart Circ Physiol 2022 Jan 14. Epub 2022 Jan 14.

Institute of Experimental Pharmacology and Toxicology, Cardiovascular Research Center, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Omecamtiv mecarbil (OM), a myosin activator, was reported to induce complex concentration- and species-dependent effects on contractile function and clinical studies indicated a low therapeutic index with diastolic dysfunction at concentrations above 1 µM. To further characterize effects of OM in a human context and under different preload conditions, we constructed a setup that allows isometric contractility analyses of human induced pluripotent stem cell (hiPSC)-derived engineered heart tissues (EHTs). The results were compared to effects of OM on the very same EHTs measured under auxotonic conditions. Read More

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January 2022

Human embryos in a dish - modeling early embryonic development with pluripotent stem cells.

Cell Regen 2022 Jan 14;11(1). Epub 2022 Jan 14.

Epigenetics and Stem Cell Biology Laboratory, National Institute of Environmental Health Sciences Research Triangle Park, Durham, NC, 27709, USA.

Stem cell-based embryo models present new opportunities to study early embryonic development. In a recent study, Kagawa et al. identified an approach to create human pluripotent stem cell-based blastoids that resemble the human blastocysts. Read More

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January 2022

Regeneration of the larval sea star nervous system by wounding induced respecification to the sox2 lineage.

Elife 2022 Jan 14;11. Epub 2022 Jan 14.

Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, United States.

The ability to restore lost body parts following traumatic injury is a fascinating area of biology that challenges current understanding of the ontogeny of differentiation. The origin of new cells needed to regenerate lost tissue, and whether they are pluripotent stem cells, tissue-specific stem cells or have de- or trans- differentiated, remains one of the most important open questions in regeneration. Additionally, it is not clearly known whether developmental gene regulatory networks (GRNs) are reused to direct specification in these cells or whether regeneration specific networks are deployed. Read More

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January 2022

Human neural cell type-specific extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain.

J Extracell Vesicles 2022 Jan;11(1):e12183

Department of Pharmacology & Experimental Therapeutics, Boston University School of Medicine, Boston, Massachusetts, USA.

In neurodegenerative diseases, extracellular vesicles (EVs) transfer pathogenic molecules and are consequently involved in disease progression. We have investigated the proteomic profiles of EVs that were isolated from four different human-induced pluripotent stem cell-derived neural cell types (excitatory neurons, astrocytes, microglia-like cells, and oligodendrocyte-like cells). Novel cell type-specific EV protein markers were then identified for the excitatory neurons (ATP1A3, NCAM1), astrocytes (LRP1, ITGA6), microglia-like cells (ITGAM, LCP1), and oligodendrocyte-like cells (LAMP2, FTH1), as well as 16 pan-EV marker candidates, including integrins and annexins. Read More

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January 2022

Insulin expression and C-peptide in type 1 diabetes subjects implanted with stem cell-derived pancreatic endoderm cells in an encapsulation device.

Cell Rep Med 2021 Dec 2;2(12):100466. Epub 2021 Dec 2.

ViaCyte Inc., San Diego, CA 92121, USA.

These preliminary data from an ongoing first-in-human phase 1/2, open-label study provide proof-of-concept that pluripotent stem cell-derived pancreatic endoderm cells (PEC-01) engrafted in type 1 diabetes patients become islet cells releasing insulin in a physiologically regulated fashion. In this study of 17 subjects aged 22-57 with type 1 diabetes, PEC-01 cells were implanted subcutaneously in VC-02 macroencapsulation devices, allowing for direct vascularization of the cells. Engraftment and insulin expression were observed in 63% of VC-02 units explanted from subjects at 3-12 months post-implant. Read More

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December 2021

General gene expression patterns and stemness of the gingiva and dental pulp.

J Dent Sci 2022 Jan 21;17(1):284-292. Epub 2021 Apr 21.

Department of Pediatric Dentistry, Yonsei University College of Dentistry, Seoul, Republic of Korea.

Background/purpose: Due to the unique properties of healing processes and cellular differentiation, the gingiva and dental pulp have attracted attention as a potential source of mesenchymal stem cells (MSCs). The purpose of this study was to obtain molecular-level information on these tissues in terms of their function and differentiation processes and investigate stemness.

Materials And Methods: Healthy gingival tissues were collected from patients ( = 9; aged 7-12 years) who underwent simple surgical procedures, and normal dental pulp tissues were obtained from patients ( = 25; aged 11-25 years) undergoing tooth extraction for orthodontic reasons. Read More

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January 2022

Comparison of 2,3,5,4'-tetrahydroxystilbene-2-O-b-D-glucoside-induced proliferation and differentiation of dental pulp stem cells in 2D and 3D culture systems-gene analysis.

J Dent Sci 2022 Jan 24;17(1):14-29. Epub 2021 Sep 24.

School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.

Background/purpose: Culture environments play a critical role in stem cell expansion. This study aimed to evaluate the effects of 2,3,5,4'-tetrahydroxystilbene-2-O-b-D-glucoside (THSG) on the proliferation and differentiation of human dental pulp stem cells (DPSCs) in 2-dimensional (2D) and 3-dimensional (3D) culture systems.

Materials And Methods: Human DPSCs were seeded in T25 flasks for 2D cultivation. Read More

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January 2022

Single-cell transcriptomics of human iPSC differentiation dynamics reveal a core molecular network of Parkinson's disease.

Commun Biol 2022 Jan 13;5(1):49. Epub 2022 Jan 13.

The Integrative Cell Signalling Group, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Esch-sur-Alzette, Luxembourg.

Parkinson's disease (PD) is the second-most prevalent neurodegenerative disorder, characterized by the loss of dopaminergic neurons (mDA) in the midbrain. The underlying mechanisms are only partly understood and there is no treatment to reverse PD progression. Here, we investigated the disease mechanism using mDA neurons differentiated from human induced pluripotent stem cells (hiPSCs) carrying the ILE368ASN mutation within the PINK1 gene, which is strongly associated with PD. Read More

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January 2022

Stem cell-derived porcine macrophages as a new platform for studying host-pathogen interactions.

BMC Biol 2022 Jan 14;20(1):14. Epub 2022 Jan 14.

The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, EH25 9RG, UK.

Background: Infectious diseases of farmed and wild animals pose a recurrent threat to food security and human health. The macrophage, a key component of the innate immune system, is the first line of defence against many infectious agents and plays a major role in shaping the adaptive immune response. However, this phagocyte is a target and host for many pathogens. Read More

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January 2022

Inhibiting mitochondrial fission rescues degeneration in hereditary spastic paraplegia neurons.

Brain 2022 Jan 13. Epub 2022 Jan 13.

Department of Biomedical Sciences, University of Illinois College of Medicine Rockford, Rockford, IL 61107, USA.

Hereditary spastic paraplegias (HSPs) are characterized by lower limb spasticity resulting from degeneration of long corticospinal axons. SPG11 is one of the most common autosomal recessive HSPs, and the SPG11 protein spatacsin forms a complex with the SPG15 protein spastizin and heterotetrameric AP5 adaptor protein complex, which includes the SPG48 protein AP5Z1. Using the integration-free episomal method, we established SPG11 patient-specific induced pluripotent stem cells (iPSCs) from patient fibroblasts. Read More

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January 2022

Generation of two iPSC lines from healthy donor with a heterozygous mutation in the VPS13B gene.

Stem Cell Res 2021 Dec 28;59:102648. Epub 2021 Dec 28.

Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia.

The human induced pluripotent stem cell (iPSC) lines, iCS-MAF1-1 and iCS-MAF1-11, were generated from fibroblasts. The donor has a heterozygous mutation in the VPS13B gene, which manifests in her child as Cohen syndrome. It is a Golgi pathology, characterized by postnatal microcephaly and delayed growth and mental development. Read More

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December 2021

in vitro models of human mast cells: How to get more and better with induced pluripotent stem cells?

J Allergy Clin Immunol 2022 Jan 10. Epub 2022 Jan 10.

Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011-EGID, F-59000 Lille, France. Electronic address:

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January 2022

Laparoscopic morphological aspects and tentative explanation of the aetiopathogenesis of isolated endometriosis of the sciatic nerve: a review based on 267 patients.

Authors:
M Possover

Facts Views Vis Obgyn 2021 Dec;13(4):369-375

Background: Endometriosis of the sciatic nerve (ESN) is considered a rare disease. How can endometriosis develop within the sciatic nerve; a structure which has nothing in common with the uterus either anatomically or functionally, and why it occurs in the absence of any retroperitoneal/parametric endometriosis, is unknown. A better understanding of the pathophysiology of this enigmatic disease may improve its diagnosis and therapy. Read More

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December 2021

Disruption of ER-mitochondria tethering and signalling in C9orf72-associated amyotrophic lateral sclerosis and frontotemporal dementia.

Aging Cell 2022 Jan 13:e13549. Epub 2022 Jan 13.

Department of Basic and Clinical Neuroscience, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London, UK.

Hexanucleotide repeat expansions in C9orf72 are the most common cause of familial amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The mechanisms by which the expansions cause disease are not properly understood but a favoured route involves its translation into dipeptide repeat (DPR) polypeptides, some of which are neurotoxic. However, the precise targets for mutant C9orf72 and DPR toxicity are not fully clear, and damage to several neuronal functions has been described. Read More

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January 2022

Stage-Specific Generation of Human Pluripotent Stem Cell Derived Lung Models to Measure CFTR Function.

Curr Protoc 2022 Jan;2(1):e341

Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, Ontario, Canada.

Human embryonic stem cells (ES) and induced pluripotent stem cells (iPSC) are powerful tools that have the potential to generate in vitro human lung epithelial cells. However, challenges in efficiency and reproducibility remain in utilizing the cells for therapy discovery platforms. Here, we optimize our previously published protocols to efficiently generate three developmental stages of the lung model (fetal lung epithelial progenitors, fLEP; immature airway epithelial spheroid, AES; air-liquid interface culture, ALI), and demonstrate its potential for cystic fibrosis (CF) drug discovery platforms. Read More

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January 2022

A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation.

iScience 2022 Jan 20;25(1):103657. Epub 2021 Dec 20.

Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan.

Pluripotent stem cell (PSC)-derived retinal sheet transplanted can form structured photoreceptor layers, contact with host bipolar cells, and transmit light signals to host retinas. However, a major concern is the presence of graft bipolar cells that may impede host-graft interaction. In this study, we used human ESC-retinas with the deletion of () gene to achieve the reduced graft ON-bipolar cells after xenotransplantation into end-stage retinal degeneration model rats. Read More

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January 2022

Establishment of large canine hepatocyte spheroids by mixing vascular endothelial cells and canine adipose-derived mesenchymal stem cells.

Regen Ther 2022 Mar 29;19:1-8. Epub 2021 Dec 29.

Laboratory of Small Animal Internal Medicine, School of Veterinary Medicine, Azabu University, Sagamihara City, Kanagawa, Japan.

Introduction: Differentiation of hepatocytes and culture methods have been investigated in dogs as a tool to establish liver transplant and drug metabolism examination systems. However, mass culture techniques for canine hepatocytes (cHep) have not been investigated, and it is necessary to construct a suitable culture system. Recently, a protocol called Bud production has attracted attention, and a mixed culture of human and mouse hepatocytes, stem cells, and artificial blood vessels significantly improved the size and formation ratio of spheroids. Read More

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Optimization of Long-Term Human iPSC-Derived Spinal Motor Neuron Culture Using a Dendritic Polyglycerol Amine-Based Substrate.

ASN Neuro 2022 Jan-Dec;14:17590914211073381

Department of Neurology and Neurosurgery, 12367Montreal Neurological Institute-Hospital, 5620McGill University, Montreal, Canada.

Human induced pluripotent stem cells (hiPSCs) derived from healthy and diseased individuals can give rise to many cell types, facilitating the study of mechanisms of development, human disease modeling, and early drug target validation. In this context, experimental model systems based on hiPSC-derived motor neurons (MNs) have been used to study MN diseases such as spinal muscular atrophy and amyotrophic lateral sclerosis. Modeling MN disease using hiPSC-based approaches requires culture conditions that can recapitulate in a dish the events underlying differentiation, maturation, aging, and death of MNs. Read More

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January 2022

Dendritic Polyglycerol Amine: An Enhanced Substrate to Support Long-Term Neural Cell Culture.

ASN Neuro 2022 Jan-Dec;14:17590914211073276

Program in Neuroengineering, Montreal Neurological Institute, 98613McGill University, Montreal, Canada.

Long-term stable cell culture is a critical tool to better understand cell function. Most adherent cell culture models require a polymer substrate coating of poly-lysine or poly-ornithine for the cells to adhere and survive. However, polypeptide-based substrates are degraded by proteolysis and it remains a challenge to maintain healthy cell cultures for extended periods of time. Read More

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January 2022

Skin Mirrors Brain: A Chance for Alzheimer's Disease Research.

Adv Exp Med Biol 2021 ;1339:371-380

Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Brandenburg Medical School Theodor Fontane and Faculty of Health Sciences Brandenburg, Dessau, Germany.

The accessibility of skin and the easy isolation of its cells and matrix components provide a valuable tool for studying the molecular factors involved in human aging. Moreover, increasing evidence corroborates the use of the skin as a model for age-associated pathological conditions in the entire body. Apparently based on the fact that the nervous system and skin share a common ectodermal origin, certain genes and molecular pathways associated with the pathomechanism of neurodegenerative diseases modify their expression with progressing skin aging. Read More

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January 2022

Inhibition of aryl hydrocarbon receptor signaling promotes the terminal differentiation of human erythroblasts.

J Mol Cell Biol 2022 Jan 11. Epub 2022 Jan 11.

Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu, China.

The aryl hydrocarbon receptor (AHR) plays an important role during mammalian embryo development. Inhibition of AHR signaling promotes the development of hematopoietic stem/progenitor cells. AHR also regulates the functional maturation of blood cells, such as T cells and megakaryocytes. Read More

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January 2022

Human amniotic mesenchymal stem cells combined with PPCNg facilitate injured endometrial regeneration.

Stem Cell Res Ther 2022 Jan 12;13(1):17. Epub 2022 Jan 12.

Department of Obstetrics and Gynecology, University-Town Hospital of Chongqing Medical University, No. 55, Daxuecheng Middle Road, Chongqing, 401331, China.

Background: Caused by the injury to the endometrial basal layer, intrauterine adhesions (IUA) are characterized by uterine cavity obliteration, leading to impaired fertility. Human amniotic mesenchymal stem cells (hAMSCs) have the potential to promote endometrial regeneration mainly through paracrine ability. PPCNg is a thermoresponsive biomaterial consisted of Poly (polyethylene glycol citrate-co-N-isopropylacrylamide) (PPCN) mixed with gelatin, which has been reported as a scaffold for stem cell transplantation. Read More

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January 2022

Modulation by DREADD reveals the therapeutic effect of human iPSC-derived neuronal activity on functional recovery after spinal cord injury.

Stem Cell Reports 2022 Jan;17(1):127-142

Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. Electronic address:

Transplantation of neural stem/progenitor cells (NS/PCs) derived from human induced pluripotent stem cells (hiPSCs) is considered to be a promising therapy for spinal cord injury (SCI) and will soon be translated to the clinical phase. However, how grafted neuronal activity influences functional recovery has not been fully elucidated. Here, we show the locomotor functional changes caused by inhibiting the neuronal activity of grafted cells using a designer receptor exclusively activated by designer drugs (DREADD). Read More

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January 2022

In vitro disease modeling of oculocutaneous albinism type 1 and 2 using human induced pluripotent stem cell-derived retinal pigment epithelium.

Stem Cell Reports 2021 Dec 24. Epub 2021 Dec 24.

Pediatric Developmental & Genetic Ophthalmology Section, Ophthalmic Genetics & Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA. Electronic address:

Oculocutaneous albinism (OCA) encompasses a set of autosomal recessive genetic conditions that affect pigmentation in the eye, skin, and hair. OCA patients display reduced best-corrected visual acuity, reduced to absent ocular pigmentation, abnormalities in fovea development, and/or abnormal decussation of optic nerve fibers. It has been hypothesized that improving eye pigmentation could prevent or rescue some of the vision defects. Read More

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December 2021

Human airway lineages derived from pluripotent stem cells reveal the epithelial responses to SARS-CoV-2 infection.

Am J Physiol Lung Cell Mol Physiol 2022 Jan 12. Epub 2022 Jan 12.

Center for Regenerative Medicine of Boston University and Boston Medical Center, Boston, MA, United States.

There is an urgent need to understand how SARS-CoV-2 infects the airway epithelium and in a subset of individuals leads to severe illness or death. Induced pluripotent stem cells (iPSCs) provide a near limitless supply of human cells that can be differentiated into cell types of interest, including airway epithelium, for disease modeling. We present a human iPSC-derived airway epithelial platform, composed of the major airway epithelial cell types, that is permissive to SARS-CoV-2 infection. Read More

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January 2022