51,111 results match your criteria play vivo

Rec(F/O/R) proteins of the nitrogen-fixing cyanobacterium Nostoc PCC7120: In silico and expression analysis.

Gene 2021 Apr 19:145663. Epub 2021 Apr 19.

Cyanobacterial Stress Biology and Biotechnology Section, Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400085, India; Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai -400094, India. Electronic address:

The high radioresistance of Nostoc sp. strain PCC7120 is indicative of a robust DNA repair pathway. In the absence of NHEJ pathway and the canonical RecBCD proteins, the RecF pathway proteins are expected to play an important role in double strand break repair in this organism. Read More

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A subpopulation of embryonic microglia respond to maternal stress and influence nearby neural progenitors.

Dev Cell 2021 Apr 19. Epub 2021 Apr 19.

Department of Medical Genetics, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada; Alberta Children's Hospital Research Institute, University of Calgary, Calgary, AB T2N 4N1, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N 4N1, Canada. Electronic address:

The interplay between hypothalamic neurons and microglia as they integrate stressors to regulate homeostasis is of growing interest. We asked if microglia in the embryonic hypothalamus were likewise stress responsive and, if so, whether their precocious activation perturbs nearby neural stem cell (NSC) programs. We performed single-cell transcriptomics to define embryonic hypothalamic microglia heterogeneity and identified four microglial subsets, including a subpopulation adjacent to NSCs that was responsive to gestational cold stress. Read More

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In vivo assessment of changes to canine airway smooth muscle volume following bronchial thermoplasty with OR-OCT.

J Appl Physiol (1985) 2021 Apr 22. Epub 2021 Apr 22.

Division of Pulmonary and Critical Medicine, Massachusetts General Hospital, United States.

The inability to assess and measure changes to the airway smooth muscle (ASM) in vivo is a major challenge to evaluating asthma and its clinical outcomes. Bronchial thermoplasty (BT) is a therapy for asthma that aims to reduce the severity of excessive bronchoconstriction by ablating ASM. While multiple long-term clinical studies of BT have produced encouraging results, the outcomes of BT treatment in practice have been variable, and questions remain regarding the selection of patients. Read More

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Bile acid sequestrants: a review of mechanism and design.

J Pharm Pharmacol 2021 Apr 22. Epub 2021 Apr 22.

Pharmaceutical College, Henan University, Kaifeng, China.

Objective: Bile acid sequestrants (BAS) are used extensively in the treatment of hypercholesterolaemia. This brief review aimed to describe the design and evaluation of three types of BAS: amphiphilic copolymers, cyclodextrin/poly-cyclodextrin and molecular imprinted polymers. The mechanisms underlying the action of BAS are also discussed. Read More

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A mesoporous polydopamine nanoparticle enables highly efficient manganese encapsulation for enhanced MRI-guided photothermal therapy.

Nanoscale 2021 Apr 23;13(13):6439-6446. Epub 2021 Mar 23.

School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.

Theranostic agents based on magnetic resonance imaging (MRI) and photothermal therapy (PTT) play an important role in tumor therapy. However, the available theranostic agents are facing great challenges such as biocompatibility, MRI contrast effect and photothermal conversion efficiency (η). In this work, mesoporous polydopamine nanoparticles (MPDAPs/Mn) were prepared on MRI and PTT combined theranostic nanoplatforms, of which the high loading manganese ions and specific surface areas enable good MRI contrast and excellent photothermal conversion efficiency, respectively. Read More

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Health effects of dietary sulfated polysaccharides from seafoods and their interaction with gut microbiota.

Compr Rev Food Sci Food Saf 2021 Apr 21. Epub 2021 Apr 21.

Department of Food Science, University of Massachusetts, Amherst, Massachusetts, USA.

Various dietary sulfated polysaccharides (SPs) have been isolated from seafoods, including edible seaweeds and marine animals, and their health effects such as antiobesity and anti-inflammatory activities have attracted remarkable interest. Sulfate groups have been shown to play important roles in the bioactivities of these polysaccharides. Recent in vitro and in vivo studies have suggested that the biological effects of dietary SPs are associated with the modulation of the gut microbiota. Read More

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Evaluation of Antibacterial Activity of Pine Tar on Periodontal Pathogenic Bacteria: An In Vitro Study.

Ethiop J Health Sci 2020 Nov;30(6):991-998

Assistant Professor, College of Dentistry, King Khalid University, Abha, Kingdom of Saudi Arabia.

Background: Periodontal pathogens play an important role in etiology and pathogenesis of periodontitis. Microbiological examination of sub gingival plaque is used at the present time in etiological research as well as in clinical treatment of periodontitis to select the appropriate antibiotic agent if indicated. Pine tar has been used for the treatment of various skin diseases. Read More

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November 2020

ALKBH4 promotes tumourigenesis with a poor prognosis in non-small-cell lung cancer.

Sci Rep 2021 Apr 21;11(1):8677. Epub 2021 Apr 21.

Laboratory of Molecular and Cellular Physiology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka, 565-0871, Japan.

The human AlkB homolog family (ALKBH) of proteins play a critical role in some types of cancer. However, the expression and function of the lysine demethylase ALKBH4 in cancer are poorly understood. Here, we examined the expression and function of ALKBH4 in non-small-cell lung cancer (NSCLC) and found that ALKBH4 was highly expressed in NSCLC, as compared to that in adjacent normal lung tissues. Read More

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A Family of Toxoplasma gondii Genes Related to GRA12 Regulate Cyst Burdens and Cyst Reactivation.

mSphere 2021 Apr 21;6(2). Epub 2021 Apr 21.

Department of Microbiology and Immunology, The Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, USA

causes a chronic infection that renders the immunocompromised human host susceptible to toxoplasmic encephalitis triggered by cyst reactivation in the central nervous system. The dense granule protein GRA12 is a major parasite virulence factor required for parasite survival during acute infection. Here, we characterized the role of four GRA12-related genes in acute and chronic stages of infection. Read More

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LncRNA CRNDE promotes the progression and angiogenesis of pancreatic cancer via miR-451a/CDKN2D axis.

Transl Oncol 2021 Apr 18;14(7):101088. Epub 2021 Apr 18.

Department of General Surgery, Suqian First Hospital, Suqian 223800, Jiangsu Province, China. Electronic address:

Background: The lncRNA colorectal neoplasia differentially expressed (lncRNA CRNDE) has been reported to play a pivotal role in various cancers. However, the expression and function of CRNDE in pancreatic cancer remain unclear. The objective of this study was to investigate the effects of CRNDE on pancreatic cancer and the underlying mechanisms. Read More

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Chemical Tagging Assisted Mass Spectrometry Analysis Enables Sensitive Determination of Phosphorylated Compounds in a Single Cell.

Anal Chem 2021 Apr 21. Epub 2021 Apr 21.

Sauvage Center for Molecular Sciences, Department of Chemistry, Wuhan University, Wuhan 430072, China.

Polar phosphorylated metabolites are involved in a variety of biological processes and play vital roles in energetic metabolism, cofactor regeneration, and nucleic acid synthesis. However, it is often challenging to interrogate polar phosphorylated metabolites and compounds from biological samples. Liquid chromatography-mass spectrometry (LC/MS) now plays a central role in metabolomic studies. Read More

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MiR-30a-5p Regulates GLT-1 Function via a PKCα-Mediated Ubiquitin Degradation Pathway in a Mouse Model of Parkinson's Disease.

ACS Chem Neurosci 2021 Apr 21. Epub 2021 Apr 21.

Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.

Glutamate excitotoxicity is caused by dysfunctional glutamate transporters and plays an important role in the pathogenesis of Parkinson's disease (PD); however, the mechanisms that underlie the regulation of glutamate transporters in PD are still not fully elucidated. MicroRNAs(miRNA), which are abundant in astrocytes and neurons, have been reported to play key roles in regulating the translation of glutamate-transporter mRNA. In this study, we hypothesized that the miR-30a-5p contributes to the pathogenesis of PD by regulating the ubiquitin-mediated degradation of glutamate transporter 1 (GLT-1). Read More

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circ_LRIG3 contributes to the progression of hepatocellular carcinoma by elevating RNF38 via sponging miR-449a.

Gen Physiol Biophys 2021 Mar;40(2):103-114

Department of Oncology, Affiliated Hospital of Xiangnan University, Chenzhou, China.

Circular RNAs (circRNAs) play crucial roles in multiple cancers, including hepatocellular carcinoma (HCC). However, the effects and molecular mechanisms of circ_LRIG3 in HCC remain barely unknown. qRT-PCR assay was employed to detect the levels of circ_LRIG3, LRIG3, miR-449a and ring finger protein 38 (RNF38). Read More

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Gynecomastia as a presenting symptom of Graves' disease in a 49-year-old man.

Endocrinol Diabetes Metab Case Rep 2021 Apr 1;2021. Epub 2021 Apr 1.

Department of Internal MedicineNorthwest Clinics, Alkmaar, the Netherlands.

Summary: Gynecomastia is a symptom with a potential high disease burden. It has a variety of underlying causes, such as malignant, drug-related or hormonal. The presence of gynecomastia can be explained in thyrotoxicosis due to a concomitant disbalance of sex hormones. Read More

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Long-access to cocaine self-administration dysregulates the glutamate synapse in the nucleus accumbens core of serotonin transporter knockout rats.

Br J Pharmacol 2021 Apr 20. Epub 2021 Apr 20.

Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Italy.

Background And Purpose: It is well established that the nucleus accumbens (NAc) and glutamate play a critical role in the motivation to take drugs of abuse. We have previously demonstrated that rats with ablation of the serotonin transporter (SERT rats) show increased cocaine intake reminiscent of compulsivity.

Experimental Approach: By comparing SERT to SERT rats, we set out to explore whether SERT deletion influences glutamate neurotransmission under control conditions as well as after short access (ShA: 1 hr. Read More

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LXA4 Inhibits Lipopolysaccharide-Induced Inflammatory Cell Accumulation by Resident Macrophages in Mice.

J Inflamm Res 2021 12;14:1375-1385. Epub 2021 Apr 12.

Department of Anesthesia and Critical Care, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, People's Republic of China.

Introduction: Alveolar macrophages that regulate the inflammatory response in lungs are the main target cell for the treatment of inflammatory pulmonary pathologies, such as acute respiratory distress syndrome (ARDS). Yolk sac derived alveolar resident macrophages play an important role in the pulmonary inflammatory response. With regards to anti-inflammatory actions, lipoxin A4 (LXA4) has been identified as an inflammatory "braking signal". Read More

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Characterization of DinJ-YafQ toxin-antitoxin module in Tetragenococcus halophilus: activity, interplay, and evolution.

Appl Microbiol Biotechnol 2021 Apr 20. Epub 2021 Apr 20.

School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.

Tetragenococcus halophilus is a moderately halophilic lactic acid bacterium widely used in high-salt food fermentation because of its coping ability under various stress conditions. Bacterial toxin-antitoxin (TA) modules are widely distributed and play important roles in stress response, but those specific for genus Tetragenococcus have never been explored. Here, a bona fide TA module named DinJ-YafQ was characterized in T. Read More

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The impact of emulsion droplet size on in vitro lipolysis rate and in vivo plasma uptake kinetics of triglycerides and vitamin D in rats.

Food Funct 2021 Apr 22;12(7):3219-3232. Epub 2021 Mar 22.

Department of Biotechnology and Food Science, Norwegian University of Science and Technology (NTNU), N-7491, Trondheim, Norway.

Emulsions play an important role in the process of triglyceride (TG) digestion (lipolysis). Through emulsification, the oil-water interface is increased by orders of magnitude. This often leads to faster and more efficient lipolysis, which is potentially beneficial for the intestinal uptake of oils and lipophilic compounds. Read More

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MiR-181b suppresses angiogenesis by directly targeting cellular communication network factor 1.

Lab Invest 2021 Apr 19. Epub 2021 Apr 19.

Clinic Center of Human Gene Research, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Angiogenesis is essential for various physiological and pathological processes. Previous studies have shown that miRNAs play an important role in blood vessel development and angiogenesis. Recent studies have suggested that miR-181b might be involved in the regulation of angiogenesis in tumors. Read More

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Scavenging of soluble and immobilized CCL21 by ACKR4 regulates peripheral dendritic cell emigration.

Proc Natl Acad Sci U S A 2021 Apr;118(17)

Chemokine Biology Laboratory, Department of Molecular and Biomedical Science, School of Biological Science, The University of Adelaide, Adelaide, SA 5005, Australia;

Leukocyte homing driven by the chemokine CCL21 is pivotal for adaptive immunity because it controls dendritic cell (DC) and T cell migration through CCR7. ACKR4 scavenges CCL21 and has been shown to play an essential role in DC trafficking at the steady state and during immune responses to tumors and cutaneous inflammation. However, the mechanism by which ACKR4 regulates peripheral DC migration is unknown, and the extent to which it regulates CCL21 in steady-state skin and lymph nodes (LNs) is contested. Read More

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Type VI secretion system and its effectors PdpC, PdpD and OpiA contribute to virulence in larvae.

Infect Immun 2021 Apr 19. Epub 2021 Apr 19.

Biozentrum, University of Basel, Switzerland

causes the deadly zoonotic disease tularemia in humans and is able to infect a broad range of organisms including arthropods, which are thought to play a major role in transmission. However, while mammalian and infection models are widely used to investigate pathogenicity, a detailed characterization of the major virulence factor, a non-canonical T6SS, in an arthropod infection model is missing. Here we use larvae to analyze the role of the T6SS and its corresponding effectors in virulence. Read More

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Damage to cardiac vasculature may be associated with breast cancer treatment-induced cardiotoxicity.

Cardiooncology 2021 Apr 19;7(1):15. Epub 2021 Apr 19.

Department of Cancer Biology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA.

Background: Breast cancer is the most common female cancer worldwide. Effective therapies including doxorubicin and trastuzumab have improved survival, but are associated with a substantial risk of cardiovascular disease. Mechanisms underlying cancer treatment-induced cardiotoxicity (CTC) are poorly understood and have largely focused on cardiomyocyte damage, although other cellular populations in the heart such as the cardiac endothelium, may play an important role in cardiac damage. Read More

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4R-cembranoid confers neuroprotection against LPS-induced hippocampal inflammation in mice.

J Neuroinflammation 2021 Apr 19;18(1):95. Epub 2021 Apr 19.

Department of Biochemistry, Universidad Central del Caribe School of Medicine, Av. Sta. Juanita, Bayamón, 00960, Puerto Rico.

Background: Chronic brain inflammation has been implicated in the pathogenesis of various neurodegenerative diseases and disorders. For example, overexpression of pro-inflammatory cytokines has been associated with impairments in hippocampal-dependent memory. Lipopolysaccharide (LPS) injection is a widely used model to explore the pathobiology of inflammation. Read More

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Gene expression under temperature stress.

New Phytol 1993 Sep;125(1):1-26

Plant Science Division, AFRC Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB, UK.

In this review, changes in plant gene expression in response to environmental stresses are discussed using the examples of high and low temperature treatments. While some changes may contribute to acclimatory processes which improve plant survival or performance under stress, others may be 'shock' responses indicative of sensitivity. The heat-shock response, which is almost ubiquitous among eukaryotic organisms, is characterized by repression of normal cellular protein synthesis mediated at both the transcriptional and the translational level, and induction of heat-shock protein (HSP) synthesis. Read More

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September 1993

Exosomes derived from mycobacterium tuberculosis-infected MSCs induce a pro-inflammatory response of macrophages.

Aging (Albany NY) 2021 Apr 19;13. Epub 2021 Apr 19.

Department of Senile Diseases, Dongying City Shengli Hospital, Dongying, Shandong Province, China.

Tuberculosis (TB) is a common infectious disease caused by Mycobacterium tuberculosis (M.tb), and macrophages serve as the primary natural host of M.tb. Read More

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Design, Synthesis and Antifungal/Anti-Oomycete Activity of Pyrazolyl Oxime Ethers as Novel Potential Succinate Dehydrogenase Inhibitors.

Pest Manag Sci 2021 Apr 19. Epub 2021 Apr 19.

Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science,China Agricultural University, Beijing, 100193, China.

Background: Succinate dehydrogenase inhibitors (SDHIs) play an increasingly important role in controlling plant diseases. However, the similar structures of SDHIs result in rapid development of cross-resistance development and a clear bottleneck of poor activity against oomycetes. Therefore, the need to seek new SDHI fungicides with novel structure is urgent. Read More

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Iptakalim alleviates synaptic damages via targeting mitochondrial ATP-sensitive potassium channel in depression.

FASEB J 2021 May;35(5):e21581

Neuroprotective Drug Discovery Key Laboratory, Jiangsu Key Laboratory of Neurodegeneration, Center for Global Health, Nanjing Medical University, Nanjing, China.

Synaptic plasticity damages play a crucial role in the onset and development of depression, especially in the hippocampus, which is more susceptible to stress and the most frequently studied brain region in depression. And, mitochondria have a major function in executing the complex processes of neurotransmission and plasticity. We have previously demonstrated that Iptakalim (Ipt), a new ATP-sensitive potassium (K-ATP) channel opener, could improve the depressive-like behavior in mice. Read More

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High-throughput microscopy reveals the impact of multifactorial environmental perturbations on colorectal cancer cell growth.

Gigascience 2021 Apr;10(4)

Lawrence J. Ellison Institute for Transformative Medicine, University of Southern California, Los Angeles, CA 90064, USA.

Background: Colorectal cancer (CRC) mortality is principally due to metastatic disease, with the most frequent organ of metastasis being the liver. Biochemical and mechanical factors residing in the tumor microenvironment are considered to play a pivotal role in metastatic growth and response to therapy. However, it is difficult to study the tumor microenvironment systematically owing to a lack of fully controlled model systems that can be investigated in rigorous detail. Read More

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LINC00941 Promotes Progression of Non-Small Cell Lung Cancer by Sponging miR-877-3p to Regulate VEGFA Expression.

Front Oncol 2021 31;11:650037. Epub 2021 Mar 31.

Department of Infectious Diseases, Affiliated Hospital 2 of Nantong University, Nantong, China.

Long noncoding RNAs (lncRNAs) play critical roles in carcinoma occurrence and metastasis. LINC00941 has been found to mediate the development of gastric cancer, and LINC00941 was negatively associated with the longer overall survival of lung adenocarcinoma patients. Herein, our aim was to investigate the effects and mechanisms of LINC00941 in NSCLC progression. Read More

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Downregulation of hsa_circ_0001836 Induces Pyroptosis Cell Death in Glioma Cells Epigenetically Upregulating NLRP1.

Front Oncol 2021 31;11:622727. Epub 2021 Mar 31.

Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Background: It has been shown that circular RNAs (circRNAs) play a vital role in the progression of glioma. Recently, hsa_circ_0001836 was found to be upregulated in glioma tissues, but the role of hsa_circ_0001836 in glioma remains unclear.

Methods: EdU staining and flow cytometry assays were used to measure the viability and death of glioma cells. Read More

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