5 results match your criteria platform bb-based

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Minimally invasive preimplantation genetic testing using blastocyst culture medium.

Hum Reprod 2019 07;34(7):1369-1379

Center of Reproductive Medicine, Shengjing Hospital of China Medical University, Huaxiang Road, Shenyang, China.

Study Question: Is minimally invasive chromosome screening (MICS) using blastocyst culture medium (BCM) sufficiently fast and accurate for preimplantation genetic testing (PGT).

Summary Answer: A new assay for MICS, named MICS-Inst achieved high-resolution, comprehensive chromosome ploidy detection using BCM.

What Is Known Already: BCM is a viable source of genomic DNA for use in PGT. Read More

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Fiber optic surface plasmon resonance biosensor for detection of PDGF-BB in serum based on self-assembled aptamer and antifouling peptide monolayer.

Biosens Bioelectron 2019 Sep 25;140:111350. Epub 2019 May 25.

Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China. Electronic address:

Herein, a home-build fiber optic surface plasmon resonance (FO-SPR) biosensing platform has been developed for highly sensitive detection of platelet-derived growth factor (PDGF-BB) based aptamer-functionalized AuNPs for signal enhancement. In this biosensor, the PDGF-BB aptamer was used to specifically capture PDGF-BB, and the antifouling peptide demonstrated great ability for resisting non-specific adsorption. After a sandwich reaction, the aptamer, PDGF-BB and aptamer-functionalized AuNPs complexes were formed on the fiber optic (FO) probe surface to significantly amplify FO-SPR signal. Read More

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September 2019

Ultrasensitive biosensing platform based on layered vanadium disulfide-graphene composites coupling with tetrahedron-structured DNA probes and exonuclease III assisted signal amplification.

J Mater Chem B 2015 Nov 21;3(41):8180-8187. Epub 2015 Sep 21.

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China.

Recently, two-dimensional layered transition metal dichalcogenide materials have been very fascinating in electrochemical fields due to their unique electronic and electrochemical properties. In the present work, a novel VS-graphene (VS-GR) composite is firstly prepared by a facile one-step hydrothermal method. A sensitive, universal and label-free electrochemical aptamer-based sensor (aptasensor) is then designed to detect the platelet-derived growth factor BB (PDGF-BB) based on VS-GR coupled with Exo III-aided autocatalytic signal amplification. Read More

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November 2015

We-Measure: Toward a low-cost portable posturography for patients with multiple sclerosis using the commercial Wii balance board.

J Neurol Sci 2015 Dec 14;359(1-2):440-4. Epub 2015 Oct 14.

Department of Neurology and Psychiatry, Sapienza University, Rome, Italy. Electronic address:

This study was aimed at investigating whether postural sway measures derived from a standard force platform were similar to those generated by a custom-written software ("We-Measure") acquiring and processing data from a commercial Nintendo balance board (BB). For this purpose, 90 patients with multiple sclerosis (MS) and 50 healthy controls (HC) were tested in a single-day session with a reference standard force platform and a BB-based system. Despite its acceptable between-device agreement (tested by visual evaluation of Bland-Altman plot), the low-cost BB-based system tended to overestimate postural sway when compared to the reference standard force platform in both MS and HC groups (on average +30% and +54%, respectively). Read More

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December 2015

Electrochemical aptameric recognition system for a sensitive protein assay based on specific target binding-induced rolling circle amplification.

Anal Chem 2010 Mar;82(6):2282-9

State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.

A reusable aptameric recognition system was described for the electrochemical detection of the protein PDGF-BB based on the target binding-induced rolling circle amplification (RCA). A complementary DNA (CDNA), linear padlock probe, and primer probe were utilized to introduce a RCA process into the aptamer-target binding event while a new aptamer was elegantly designed via lengthening the original aptamer by the complement to the CDNA. The aptameric sensing system facilitates the integration of multiple functional elements into a signaling scheme: a unique electrochemical technique, an attractive RCA process, reversible DNA hybridization, and desirable aptameric target recognition. Read More

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