4,814 results match your criteria platelet storage


In vitro characteristics of cryopreserved platelet concentrates using fresh frozen or lyophilized plasma.

Transfus Clin Biol 2022 Jan 12. Epub 2022 Jan 12.

Etablissement Français du Sang, 20 avenue du Stade-de-France, 93218 La Plaine Saint-Denis, France. Electronic address:

Background And Objectives: Six per cent dimethyl sulfoxide (DMSO) cryopreservation of platelet concentrates (PCs) allows longer storage of PCs but requires time-consuming post-thaw washing. An alternative process based on removing supernatant before freezing has been implemented in several centres worldwide. We assessed the in vitro characteristics of cryopreserved PCs (CPPs) prepared according to this latest process using either French lyophilized plasma (FLyP) or fresh frozen plasma (FFP) for reconstitution. Read More

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January 2022

Evaluation of platelet concentrates prepared from whole blood donations with collection times between 12 and 15 min: The BEST Collaborative study.

Vox Sang 2022 Jan 12. Epub 2022 Jan 12.

Hospital Israelita Albert Einstein, São Paulo, Brazil.

Background And Objectives: In many countries, whole blood (WB) donations with collection times between 12 and 15 min are not allowed to be used for platelet concentrates (PC). Since the development of guidelines, many process-related changes have been introduced. We aimed to determine the effect of WB with long collection times on PC quality. Read More

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January 2022

Agitation-dependent biomechanical forces modulate GPVI receptor expression and platelet adhesion capacity during storage.

Thromb J 2022 Jan 12;20(1). Epub 2022 Jan 12.

Charles Perkins Centre, The University of Sydney, Camperdown, NSW, Australia.

Background: Continuous agitation during storage slows down the platelet storage lesions. However, in special circumstances, manual-mixing can be alternatively used to store products for short time periods without compromising platelet quality. Based on this finding, and given the role of shear stress in modulating receptor expression, we were interested in comparing the levels of platelet adhesion receptor, GPVI and platelet adhesion capacity under each storage condition. Read More

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January 2022

Metal-Organic Framework Reinforced Acrylic Polymer Marine Coatings.

Materials (Basel) 2021 Dec 21;15(1). Epub 2021 Dec 21.

Department of Naval Architecture and Ocean Engineering, Pusan National University, Busan 46241, Korea.

Metal-organic frameworks (MOFs), a class of crystalline, porous, 3D materials synthesized by the linking of metal nodes and organic linkers are rapidly emerging as attractive materials in gas storage, electrodes in batteries, super-capacitors, sensors, water treatment, and medicine etc. However the utility of MOFs in coatings, especially in marine coatings, has not been thoroughly investigated. In this manuscript we report the first study on silver MOF (Ag-MOF) functionalized acrylic polymers for marine coatings. Read More

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December 2021

Time from apheresis platelet donation to cold storage: Evaluation of platelet quality and bacterial growth.

Transfusion 2022 Jan 7. Epub 2022 Jan 7.

American Red Cross, Holland Lab for the Biomedical Sciences, Rockville, Maryland, USA.

Background: Cold storage reduces posttransfusion survival of platelets; however, it can improve platelet activation, lower risk of bacterial contamination, and extend shelf-life compared to room temperature (RT) storage. To facilitate large-scale availability, manufacturing process optimization is needed, including understanding the impact of variables on platelet potency and safety. Short time requirements from collection to storage is challenging for large blood centers to complete resuspension and qualify platelets for production. Read More

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January 2022

Glycoprotein V : the unsolved GPV puzzle.

Platelets 2022 Jan 7:1-8. Epub 2022 Jan 7.

Institut National de la Santé et de la Recherche Médicale, Etablissement Français du Sang Grand Est, Unité Mixte de Recherche-S 1255, Fédération de Médecine Translationnelle de Strasbourg, Université de Strasbourg, Strasbourg, France.

Glycoprotein V (GPV) is a highly expressed 82 KDa platelet surface transmembrane protein which is loosely attached to the GPIb-IX complex. Despite remaining questions concerning its function, GPV presents several unique features which have repercussions in hematology, atherothrombosis, immunology and transfusion. GPV is specifically expressed in platelets and megakaryocytes and is an ideal marker and reporter gene for the late stages of megakaryopoiesis. Read More

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January 2022

3D printed carboxymethyl cellulose scaffolds for autologous growth factors delivery in wound healing.

Carbohydr Polym 2022 Feb 23;278:118924. Epub 2021 Nov 23.

Departamento de Farmacología, Farmacia y Tecnología Farmacéutica, I+D Farma (GI-1645), Facultad de Farmacia and Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain. Electronic address:

This work aims to use carboxymethyl cellulose (CMC) as main structural and functional component of 3D printed scaffolds for healing of diabetic wounds. Differently from previous inks involving small contents in CMC, herein sterile (steam-heated) concentrated CMC solely dispersions (10-20%w/v) were screened regarding printability and fidelity properties. CMC (15%w/v)-citric acid inks showed excellent self-healing rheological properties and stability during storage. Read More

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February 2022

Role of Circulating Microparticles in Type 2 Diabetes Mellitus: Implications for Pathological Clotting.

Semin Thromb Hemost 2021 Dec 27. Epub 2021 Dec 27.

Department of Physiological Sciences, Faculty of Science, Stellenbosch University, Stellenbosch, South Africa.

Type 2 diabetes mellitus (T2DM) is a multifactorial chronic metabolic disease characterized by chronic hyperglycemia due to insulin resistance and a deficiency in insulin secretion. The global diabetes pandemic relates primarily to T2DM, which is the most prevalent form of diabetes, accounting for over 90% of all cases. Chronic low-grade inflammation, triggered by numerous risk factors, and the chronic activation of the immune system are prominent features of T2DM. Read More

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December 2021

Mass cytometry of platelet-rich plasma: a new approach to analyze platelet surface expression and reactivity.

Platelets 2021 Dec 27:1-8. Epub 2021 Dec 27.

Department of Internal Medicine I, School of Medicine, University Hospital Rechts der Isar, Technical University of Munich, Munich, Germany.

Mass cytometry (CyTOF) is a new technology that allows the investigation of protein expression at single cell level with high resolution. While several protocols are available to investigate leukocyte expression, platelet staining and analysis with CyTOF have been described only from whole blood. Moreover, available protocols do not allow sample storage but require fresh samples to be obtained, processed, and measured immediately. Read More

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December 2021

[Clinical comprehensive evaluation of Naoxintong Capsules in treatment of cerebral infarction with Qi deficiency and blood stasis syndrome and coronary heart disease angina pectoris].

Zhongguo Zhong Yao Za Zhi 2021 Dec;46(23):6087-6095

Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences Beijing 100700, China.

Through investigating the current research on the effect of Naoxintong Capsules in the treatment of cerebral infarction with Qi deficiency and blood stasis syndrome and coronary heart disease angina pectoris, this study conducted a clinical comprehensive evaluation in "6+1" dimensions [safety, effectiveness, economy, innovation, suitability, accessibility, and characteristics of traditional Chinese medicine(TCM)], so as to highlight the advantages and clinical value of Naoxintong Capsules. By combining qualitative and quantitative methods, we used the multi-criteria decision analysis(MCDA) model to measure each dimension, and the results thereby were divided into four grades of A, B, C, and D in high-to-low order. Through literature review and evaluation, the adverse effects of Naoxintong Capsules are mainly caused by the gastrointestinal system. Read More

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December 2021

Detection of Sepsis in Platelets Using MicroRNAs and Membrane Antigens.

Genes (Basel) 2021 Nov 25;12(12). Epub 2021 Nov 25.

Molecular Biology Laboratory, Ophir Loyola Hospital, Belém 66063-240, PA, Brazil.

The present study proposes to legitimize in sepsis a characteristic found in platelets that suffer storage lesions in blood banks, which is the increased expression of miRNA miR-320a in relation to miR-127. Under physiologically normal conditions, an inverse relationship is observed. The aim of this study was to verify whether the analysis of miR-320a and miR-127 expression in platelets could detect a decrease in their viability and function due to the presence of pathogens in the blood of patients hospitalized in the Intensive Care Unit. Read More

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November 2021

An Investigation of ABO Blood Type and the Platelet Delta Granule Storage Pool.

Clin Appl Thromb Hemost 2021 Jan-Dec;27:10760296211068818

University of Toledo, Toledo, OH, USA.

Individuals with bleeding tendencies are more likely to have blood type O than blood types A, B, or AB. Platelet storage pool deficiencies are a lesser-known group of bleeding disorders which often go undiagnosed and may account for a significant number of patients with unexplained bleeding defects. We hypothesized that patients with platelet δ-storage pool deficiency might also have a predominance of type O blood. Read More

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December 2021

Acquired platelet storage container leaks and contamination with environmental bacteria: A preventable cause of bacterial sepsis.

Transfusion 2021 Dec 20. Epub 2021 Dec 20.

Clinical Research and Medical Affairs, Cerus Corporation, Concord, California, USA.

Background: Apheresis platelets (AP) may be contaminated by environmental bacteria via container defects acquired during processing, transport, storage, or transfusion, as highlighted by a recent series of septic reactions related to Acinetobacter spp. and other bacterial strains.

Study Design And Methods: The frequency and nature of acquired container defect reports to one manufacturer were evaluated from January 2019 to July 2020. Read More

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December 2021

Novel Management and Screening Approaches for Haematological Complications of Gaucher's Disease.

J Blood Med 2021 7;12:1045-1056. Epub 2021 Dec 7.

Foundation FEETEG, Zaragoza, Spain.

Purpose: Gaucher disease (GD) is the most common lysosomal storage disorder. The principal manifestations for its diagnosis and further monitoring include haematological manifestations such as anaemia, thrombocytopaenia, spleen enlargement, and bleeding disorders, among others. This review aims to summarise and update the role of haematological complications in GD diagnosis and follow-up, describe their management strategies, and to use these indicators as part of the diagnostic approach. Read More

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December 2021

Regulated upon activation, normal T cells expressed and secreted (CCL5) in platelet concentrate: Role of mode of preparation and duration of storage.

Asian J Transfus Sci 2021 Jul-Dec;15(2):189-194. Epub 2021 Nov 1.

Surat Raktadan Kendra and Research Centre, Surat, Gujarat, India.

Background And Objectives: Platelet concentrates (PCs) can be prepared in several different ways, and they can be stored over few days before the use. Regulated on activation, normal T cells expressed and secreted (RANTES) levels in these concentrates may vary depending on the type of preparation and duration of storage of this component. We measured RANTES levels in platelet supernatants in different preparations and with different storage duration. Read More

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November 2021

Buffy coat pooled platelet concentrate: A new age platelet component.

Asian J Transfus Sci 2021 Jul-Dec;15(2):125-132. Epub 2021 Nov 1.

Department of Biochemistry, PGIMER, Chandigarh, India.

Background: Buffy coat pooled platelet concentrate (BCPP) is a new blood component mainly used in Europe, which has good attributes of both random donor platelets and apheresis platelets in terms of high platelet count, leukoreduced and available in emergency. We planned this study to compare quality parameters and biochemical activation markers among buffy coat pooled platelets and apheresis platelet concentrate (AP-PC) to establish the quality and safety of this new blood component during storage.

Materials And Methods: Three different preparations of BCPP were prepared: Nonleukoreduced (BCPP Part A), leukoreduced (BCPP Part B), and leukoreduced with platelet additive solution (PAS) (BCPP Part C) using a pool of 15 ABO-matched, nonreactive buffy-coats in each experiment to avoid any donor-related variations. Read More

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November 2021

Lyophilised Platelet-Rich Fibrin: Physical and Biological Characterisation.

Molecules 2021 Nov 25;26(23). Epub 2021 Nov 25.

Faculty of Dentistry, Sir John Walsh Research Institute, University of Otago, P.O. Box 56, Dunedin 9054, New Zealand.

Background: Platelet-rich fibrin (PRF) has gained popularity in craniofacial surgery, as it provides an excellent reservoir of autologous growth factors (GFs) that are essential for bone regeneration. However, the low elastic modulus, short-term clinical application, poor storage potential and limitations in emergency therapy use restrict its more widespread clinical application. This study fabricates lyophilised PRF (Ly-PRF), evaluates its physical and biological properties, and explores its application for craniofacial tissue engineering purposes. Read More

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November 2021

Effects and treatment applications of polymeric nanoparticles on improving platelets' storage time: a review of the literature from 2010 to 2020.

Blood Res 2021 Dec;56(4):215-228

Pediatric Congenital Hematologic Disorders Research Center, Shahid Beheshti University of Medical Sciences and Iran Blood Transfusion Organization, Tehran, Iran.

Maintaining the quality of platelet products and increasing their storage time are priorities for treatment applications. The formation of platelet storage lesions that limit the storage period and preservation temperature, which can prepare a decent environment for bacterial growth, are the most important challenges that researchers are dealing with in platelet preservation. Nanotechnology is an emerging field of science that has introduced novel solutions to resolve these problems. Read More

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December 2021

Assessment of apheresis platelets during 5 days of storage: A National Cancer Institute, Cairo University experience.

Transfus Apher Sci 2021 Nov 25:103327. Epub 2021 Nov 25.

Clinical Pathology Department, National Cancer Institute, Cairo University, Cairo, Egypt.

Background: Platelet transfusion therapy is widely used to prevent hemorrhage in patients with thrombocytopenia and platelet disorders. The platelet concentrate (PC) quality is affected by increased storage time, as reflected in the decreased number of platelets, morphological changes, and impaired functions. This study aimed to analyze the impact of 5 days storage on platelets count and the expression of CD63, and Annexin V as activation markers during PC storage. Read More

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November 2021

Impact of different pathogen reduction technologies on the biochemistry, function, and clinical effectiveness of platelet concentrates: An updated view during a pandemic.

Transfusion 2022 01 6;62(1):227-246. Epub 2021 Dec 6.

Global Blood Advisor, adjunct Professor, Arizona State University, USA.

Standard platelet concentrates (PCs) stored at 22°C have a limited shelf life of 5 days. Because of the storage temperature, bacterial contamination of PCs can result in life-threatening infections in transfused patients. The potential of blood components to cause infections through contaminating pathogens or transmitting blood-borne diseases has always been a concern. Read More

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January 2022

The 4-Year Experience with Implementation and Routine Use of Pathogen Reduction in a Brazilian Hospital.

Pathogens 2021 Nov 18;10(11). Epub 2021 Nov 18.

Hospital Sírio-Libanês Blood Bank, São Paulo 01308-050, Brazil.

(1) Background: We reviewed the logistics of the implementation of pathogen reduction (PR) using the INTERCEPT Blood System™ for platelets and the experience with routine use and clinical outcomes in the patient population at the Sírio-Libanês Hospital of São Paulo, Brazil. (2) Methods: Platelet concentrate (PC), including pathogen reduced (PR-PC) production, inventory management, discard rates, blood utilization, and clinical outcomes were analyzed over the 40 months before and after PR implementation. Age distribution and wastage rates were compared over the 10 months before and after approval for PR-PC to be stored for up to seven days. Read More

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November 2021

Comparison of Two Alternative Procedures to Obtain Packed Red Blood Cells for β-Thalassemia Major Transfusion Therapy.

Biomolecules 2021 11 4;11(11). Epub 2021 Nov 4.

Transfusion Medicine Unit, Azienda USL-IRCCS di Reggio Emilia, 42123 Reggio Emilia, Italy.

β-thalassemia major (βTM) patients require frequent blood transfusions, with consequences that span from allogenic reactions to iron overload. To minimize these effects, βTM patients periodically receive leucodepleted packed red blood cells (P-RBCs) stored for maximum 14 days. The aim of this study was to compare two alternative routine procedures to prepare the optimal P-RBCs product, in order to identify differences in their content that may somehow affect patients' health and quality of life (QoL). Read More

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November 2021

Silanization of nanographene platelets improves interaction with the dentin bonding resin matrix and enhances interfacial bond integrity to dentin.

Biomater Sci 2021 Dec 7;9(24):8335-8346. Epub 2021 Dec 7.

UWA Dental School, University of Western Australia, 17 Monash Avenue, Nedlands, WA 6009, Australia.

This study synthesized and characterized graphene nanoplatelets silanized with 3-(trimethoxysilyl)propyl methacrylate (MPS-GNP) for morphological and chemical characteristics. In addition, we modified a dentin bonding agent using different concentrations of MPS-GNP to study its interaction within the resin matrix of the adhesive, degree of conversion (DC), biological, and mechanical properties after bonding to tooth. Both 0. Read More

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December 2021

Prolonged storage of purified granulocyte concentrates: Introduction of a new purification method.

Transfusion 2022 Jan 16;62(1):194-204. Epub 2021 Nov 16.

Artcline GmbH, Rostock, Germany.

Background: Use of donor granulocyte concentrate (GC) has been limited due to its short storage time of 6-24 h, which is partially due to residual red blood cells (RBCs) and platelets and the resulting lactate production leading to an acidotic milieu. To increase this storage time, we developed a closed system procedure compatible with standard blood bank technologies to remove RBC and platelets and to enrich the GC.

Methods: Standard GCs (sGCs) were sedimented, washed twice with 0. Read More

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January 2022

Effect of bedside filtration on aggregates from cold-stored whole blood-derived platelet-rich plasma and apheresis platelet concentrates.

Transfusion 2022 Jan 15;62(1):22-27. Epub 2021 Nov 15.

Bloodworks Northwest Research Institute, Seattle, Washington, USA.

Background: The current approach to manufacture cold-stored platelets (CSP) replicates that of room temperature-stored platelets (RSP). However, this production method is associated with aggregate formation in CSP, a major pitfall that leads to significant wastage. We hypothesized that isolating platelets from whole blood as platelet-rich plasma (PRP) and storing them at a lower concentration reduces aggregates and that conventional bedside transfusion filtration removes CSP aggregates. Read More

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January 2022

Transregional autologous serum eye drop provision by a large German Red Cross Blood Donation Service.

Transfus Apher Sci 2021 Nov 9:103316. Epub 2021 Nov 9.

German Red Cross Blood Donation Service West, Center for Transfusion Medicine Breitscheid, Ratingen, Germany.

Background: The Blood Donation Service West serves North Rhine-Westphalia (NRW), Rhineland-Palatinate (RP), and Saarland, an area of 56,500 km. In addition to routine red blood cell concentrates, plasma, and platelets, special products are provided. Since 2014, this has included autologous serum eye drops (ASED) for topical use in patients suffering from different illnesses accompanied by dry eye disease. Read More

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November 2021

Impact of short-term storage of plasma samples on quantitation of ultra-low levels of interleukin-6.

J Int Med Res 2021 Nov;49(11):3000605211056846

Department of Laboratory Medicine, 118108Shinshu University Hospital, Shinshu University Hospital, Asahi, Matsumoto, Nagano, Japan.

Objective: To quantitate plasma interleukin-6 (IL-6) levels in healthy individuals and to clarify how these levels are affected by blood sample handling procedures during short-term storage.

Methods: Ethylenediaminetetraacetic acid (EDTA)-treated plasma samples were simultaneously collected from 14 healthy individuals and stored on ice prior to analysis of the IL-6 levels. White blood cells (WBCs), red blood cells, and platelets were counted immediately after blood collection. Read More

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November 2021

Recovery of platelet-rich red blood cells and acquisition of convalescent plasma with a novel gravity-driven blood separation device.

Transfus Med 2021 Nov 10. Epub 2021 Nov 10.

Department of Blood Cell Research, Sanquin Research, Amsterdam, The Netherlands.

Objectives: Our objectives were to determine the separation characteristics and blood product quality of a gravity-driven microfiltration blood separation system (HemoClear, The Netherlands).

Background: A range of centrifugal blood separation devices, including intraoperative cell salvage devices (cell savers) and apheresis machines, are available to assist in preparing both allogenic and autologous blood products. These devices are expensive to operate and require extensive training. Read More

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November 2021

Donor citrate reactions influence the phenotype of apheresis platelets following storage.

Transfusion 2021 Nov 10. Epub 2021 Nov 10.

Research and Development, Australian Red Cross Lifeblood, Sydney (Alexandria), New South Wales, Australia.

Background: Platelet collection and processing methods, as well as donor attributes, can influence platelet function and quality during ex vivo storage. In this study, activation and procoagulant responses in platelets collected from donors experiencing a citrate reaction (CR) were investigated.

Study Design And Methods: Apheresis platelet components (n = 54) were stored in 100% autologous plasma and tested on days 1 and 5 post-collection. Read More

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November 2021

Evaluating apheresis platelets at reduced dose as a contingency measure for extreme shortages.

Transfusion 2022 Jan 10;62(1):173-182. Epub 2021 Nov 10.

Component Development Laboratory, NHS Blood and Transplant, Cambridge, UK.

Background: The COVID19 pandemic highlights the need for contingency planning in the event of blood shortages. To increase platelet supply, we assessed the operational impact and effect on platelet quality of splitting units prior to storage.

Study Design And Methods: Using production figures, we modeled the impact on unit numbers, platelet counts, and volumes of splitting only apheresis double donations into three units (yielding ⅔ doses), or all standard dose units in half. Read More

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January 2022