60,488 results match your criteria plasminogen activator


Toxicon research paper Structural and bioinformatic analyses of Azemiops venom serine proteases reveal close phylogeographic relationships to pitvipers from eastern China and the New World.

Toxicon 2021 May 3. Epub 2021 May 3.

Institute of Biological Chemistry,Academia Sinica,Taipei, Taiwan.

The semi-fossil and pit-less Azemiops feae is possibly the most primitive crotalid species. Here, we have cloned and sequenced cDNAs encoding four serine proteases (vSPs) from the venom glands of Chinese A. feae. Read More

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Puerperium Stroke and Subsequent Tissue-Type Plasminogen Activator-Induced Hemorrhage: A Case Report.

A A Pract 2021 May 6;15(5):e01459. Epub 2021 May 6.

From the Anesthesiology Institute.

We report a case of ischemic stroke in an apparently healthy 35-year-old gravida 2 para 1 who was treated with tissue-type plasminogen activator (tPA) 9 hours after vaginal delivery that resulted in severe hemorrhage. Limited data suggest use of thrombolytics in pregnancy is safe, but there is a paucity of evidence assessing their use immediately postpartum. We describe successful combination of tPA with endovascular mechanical thrombectomy (EMT) for treatment of postpartum stroke, which was followed by extensive uterine bleeding. Read More

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Remote Ischemic Conditioning and Stroke Recovery.

Neurorehabil Neural Repair 2021 May 6:15459683211011224. Epub 2021 May 6.

University of Ottawa, ON, Canada.

Remote ischemic conditioning (RIC) is a noninvasive procedure whereby several periods of ischemia are induced in a limb. Although there is growing interest in using RIC to improve stroke recovery, preclinical RIC research has focused exclusively on neuroprotection, using male animals and the intraluminal suture stroke model, and delivered RIC at times not relevant to either brain repair or behavioral recovery. In alignment with the Stroke Recovery and Rehabilitation Roundtable, we address these shortcomings. Read More

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The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury.

J Ophthalmol 2021 15;2021:5565178. Epub 2021 Apr 15.

Department of Ophthalmology, Tianjin Medical University General Hospital, Tianjin, China.

Purpose: To evaluate the effects of intravitreal anti-VEGF agents in a rabbit model of open-globe injury (OGI).

Methods: OGI was induced in the right eyes of 75 Belgian rabbits by making 5 mm circumferential incision placed 6 mm behind the limbus. The rabbits were divided into 4 groups: control ( = 5), OGI group ( = 40), and intravitreal Ranibizumab and Conbercept ( = 15 each). Read More

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Cadmium induces plasminogen activator inhibitor-1 via Smad2/3 signaling pathway in human endothelial EA.hy926 cells.

J Toxicol Sci 2021 ;46(5):249-253

Faculty of Pharmaceutical Sciences, Toho University.

Modulation of the blood coagulation fibrinolytic system is an essential function of vascular endothelial cells. Tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) are major fibrinolytic regulatory proteins synthesized by vascular endothelial cells; fibrinolytic activity is dependent on the balance between these proteins. Previously, we have reported that cadmium, an initiator of ischemic heart disease, induces PAI-1 expression and suppresses fibrinolytic activity in cultured human vascular endothelial cells. Read More

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January 2021

Predictors of Outcomes in Patients With Mild Ischemic Stroke Symptoms: MaRISS.

Stroke 2021 May 5:STROKEAHA120032809. Epub 2021 May 5.

Department of Neurology, Massachusetts General Hospital/Harvard Medical School, Boston (L.H.S.).

Background And Purpose: Although most strokes present with mild symptoms, these have been poorly represented in clinical trials. The objective of this study is to describe multidimensional outcomes, identify predictors of worse outcomes, and explore the effect of thrombolysis in this population.

Methods: This prospective observational study included patients with ischemic stroke or transient ischemic attack, a baseline National Institutes of Health Stroke Scale (NIHSS) score 0 to 5, presenting within 4. Read More

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Imbalance in Coagulation/Fibrinolysis Inhibitors Resulting in Extravascular Thrombin Generation in Gliomas of Varying Levels of Malignancy.

Biomolecules 2021 Apr 29;11(5). Epub 2021 Apr 29.

Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.

Neoplastic processes are integrally related to disturbances in the mechanisms regulating hemostatic processes. Brain tumors, including gliomas, are neoplasms associated with a significantly increased risk of thromboembolic complications, affecting 20-30% of patients. As gliomas proliferate, they cause damage to the brain tissue and vascular structures, which leads to the release of procoagulant factors into the systemic circulation, and hence systemic activation of the blood coagulation system. Read More

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Reverse tracing anti-thrombotic active ingredients from dried Rehmannia Radix based on multidimensional spectrum-effect relationship analysis of steaming and drying for nine cycles.

J Ethnopharmacol 2021 May 1:114177. Epub 2021 May 1.

Research Center for Traceability and Standardization of TCMs, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 211198, China; State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 210009, China. Electronic address:

Ethnopharmacological Relevance: In traditional Chinese medicine (TCM) and modern pharmacodynamics, dried Rehmannia Radix (DRR) possesses prominent anti-thrombotic activity that decreases after processing by nine steaming and drying cycles to develop processed Rehmannia Radix (PRR). Due to the complexity of the DRR components, the chemical mechanism leading to efficacy changes of DRR caused by processing is still unclear.

Aim Of Study: This study aimed to trace the anti-thrombotic active compounds of DRR and different degrees of processed RR (PRR) and to evaluate the synergistic effects among different active components. Read More

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Red cell index: A novel biomarker for 3-month mortality in acute ischemic stroke patients treated with intravenous thrombolysis.

Brain Behav 2021 May 4:e02170. Epub 2021 May 4.

Department of Neurology, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Background: The red cell index (RCI) was described as a biomarker for evaluating respiratory function in previous studies, but the relationship between RCI and stroke, remained a mystery. The present study aimed to probe the association between RCI at 24-hr and 3-month mortality and functional outcomes among acute ischemic stroke (AIS) patients treated with recombinant tissue plasminogen activator (r-tPA).

Methods: A total of 217 AIS patients between January 2016 and January 2019 were recruited in this retrospective study. Read More

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Changes of fibrinolytic system in thrombolytic resuscitation of pulmonary thromboembolism-induced cardiac arrest model.

Int Wound J 2021 May 4. Epub 2021 May 4.

Department of Emergency, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

The objective of this study is to explore the changes in the coagulation and fibrinolysis system in an animal model with pulmonary embolism after cardiopulmonary bypass and to provide a theoretical basis for clinical practice. An animal model of cardiac arrest due to pulmonary embolism was established for venous thrombus (10-15 mL) in the left external jugular vein of 21 pigs. Computed tomography (CT) pulmonary arteriography was performed after the recovery of the underlying state, cardiac arrest state and spontaneous circulation, and then thrombolysis and cardiopulmonary resuscitation (recombinant tissue plasminogen activator [t-PA] 50 mg) were performed immediately. Read More

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2,5TT genotype of the MMP-9-1562C/T polymorphism may be a risk factor for thrombolytic therapy-induced hemorrhagic complications after acute ischemic stroke.

Pharmacotherapy 2021 May 4. Epub 2021 May 4.

St. Sava Hospital, Belgrade, Serbia.

Introduction: Levels of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) influence recombinant tissue plasminogen activator (rtPA) therapy response in patients with acute ischemic stroke (AIS). Serum levels of MMPs and TIMPs along with the expression of genes coding these proteins are related to the recovery and appearance of adverse effects (AE) after AIS. Consequently, it is important to explore whether polymorphisms in regulatory sequences of MMPs and TIMPs are associated with rtPA response in AIS patients. Read More

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Thrombolysis of Pulmonary Emboli via Endobronchial Ultrasound-Guided Transbronchial Needle Injection.

Ann Thorac Surg 2020 Oct 24. Epub 2020 Oct 24.

Division of Thoracic Surgery, Toronto General Hospital, University Health Network, Ontario, Canada. Electronic address:

Background: Endobronchial ultrasound-guided transbronchial needle injection (EBUS-TBNI) is a novel technique for treating peribronchial targets. The aim of this study was to evaluate preliminary feasibility of thrombolysis of pulmonary emboli via EBUS-TBNI.

Methods: Yorkshire pigs (30-48 kg) were anesthetized and mechanically ventilated. Read More

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October 2020

Sexual Dimorphism in Hepatocyte Xenograft Models.

Cell Transplant 2021 Jan-Dec;30:9636897211006132

Department of Gastroenterology and Hepatology, 6993Erasmus University Medical Center, Rotterdam, The Netherlands.

Humanized liver mouse models are crucial tools in liver research, specifically in the fields of liver cell biology, viral hepatitis and drug metabolism. The livers of these humanized mouse models are repopulated by 3-dimensional islands of fully functional primary human hepatocytes (PHH), which are notoriously difficult to maintain in vitro. As low efficiency and high cost hamper widespread use, optimization is of great importance. Read More

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Retinol-binding protein 4 and plasminogen activator inhibitor-1 as potential prognostic biomarkers of non-allergic asthma caused by obesity in adolescents.

Allergol Immunopathol (Madr) 2021 1;49(3):21-29. Epub 2021 May 1.

Universidad Nacional Autónoma de México, Programa de Maestría y Doctorado en Ciencias Médicas, Odontológicas y de la Salud, Mexico City, Mexico;

Background: Non-allergic asthma caused by obesity is a complication of the low-grade chronic inflammation inherent in obesity. Consequently, the serum concentrations of adipokines such as retinol-binding protein 4 (RBP4) and plasminogen activator inhibitor-1 (PAI-1) increase. No gold standard molecule for the prediction of non-allergic asthma among obese patients has been identified. Read More

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Compartment syndrome following use of tissue plasminogen activator for frostbite in the setting of concomitant diaphyseal tibia fracture.

OTA Int 2020 Jun 22;3(2):e079. Epub 2020 May 22.

Department of Orthpaedic Surgery, MetroHealth Medical Center, Cleveland, OH.

Background: Tissue plasminogen activator (tPA) is a thrombolytic agent increasingly being employed for the treatment of acute frostbite. Although tPA has been shown with success to increase digit salvage rates, data on potential complications, including risk of hemorrhage, is limited. As a result, acute trauma is considered a contraindication to use in many institution-based protocols. Read More

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A Serpin With a Finger in Many PAIs: PAI-1's Central Function in Thromboinflammation and Cardiovascular Disease.

Front Cardiovasc Med 2021 16;8:653655. Epub 2021 Apr 16.

Aberdeen Cardiovascular and Diabetes Centre, Institute of Medical Sciences, School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, United Kingdom.

Plasminogen activator inhibitor 1 (PAI-1) is a member of the serine protease inhibitor (serpin) superfamily. PAI-1 is the principal inhibitor of the plasminogen activators, tissue plasminogen activator (tPA), and urokinase-type plasminogen activator (uPA). Turbulence in the levels of PAI-1 tilts the balance of the hemostatic system resulting in bleeding or thrombotic complications. Read More

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Angioedema after rt-PA infusion led to airway emergency: a case report of rescue treatment with fresh frozen plasma.

Braz J Anesthesiol 2021 Apr 28. Epub 2021 Apr 28.

Ospedale Maggiore, Intensive Care and Prehospital Emergency Service, Department of Anesthesia, Bologna, Italy.

The authors report the case of a 71-year-old woman presented to the Emergency Department with AIS. She was treated with rt-PA and interventional endovascular revascularization and developed rapidly progressing angioedema that led to emergency intubation. The standard treatment was not very effective and the swelling improved after infusion of fresh frozen plasma. Read More

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Tumour-derived substrate-adherent cells promote neuroblastoma survival through secreted trophic factors.

Mol Oncol 2021 May 1. Epub 2021 May 1.

School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China.

Neuroblastoma (NB) is the most common extracranial solid tumour in children. NB is highly heterogeneous and is comprised of a mixture of neuroblastic cancer cells and stromal cells. We previously reported that N-type cells (neuroblastic cells) and S-type cells (substrate-adherent cells) in the SK-N-SH cell line shared almost identical genetic backgrounds. Read More

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Functional and structural outcome after vitrectomy combined with subretinal rtPA Injection with or without additional intravitreal Bevacizumab injection for submacular hemorrhages.

PLoS One 2021 30;16(4):e0250587. Epub 2021 Apr 30.

Depatment of Ophthalmology, Knappschaft Hospital Saar, Sulzbach/Saar, Germany.

Background: To analyze the functional and anatomical outcome after vitrectomy with subretinal rtPA (recombinant tissue plasminogen activator) combined with or without an intravitreal Bevacizumab injection.

Patients And Methods: Retrospective, consecutive case series of 31 pseudophakic patients with submacular hemorrhage (SMH) due to neovascular age-related macular degeneration (AMD) treated with vitrectomy, subretinal rtPA and pneumatic air displacement with or without an additional intravitreal Bevacizumab injection. The primary endpoints were best-corrected visual acuity (BCVA), and central macular thickness (CMT) measured by SD‑OCT. Read More

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Urokinase-type plasminogen activator-mediated crosstalk between N-cadherin and β-Catenin promotes wound healing.

J Cell Sci 2021 Apr 30. Epub 2021 Apr 30.

Division of Neuropharmacology and Neurologic Diseases, Yerkes National Primate Research Center; Atlanta, GA, USA.

Urokinase-type plasminogen activator (uPA) is a serine proteinase that in the central nervous system induces astrocytic activation. β-Catenin is a protein that links the cytoplasmic tail of cadherins to the actin cytoskeleton, thus securing the formation of cadherin-mediated cell adhesion complexes. Disruption of cell-cell contacts leads to the detachment of β-Catenin from cadherins, which is then degraded by the proteasome following its phosphorylation by GSK3β. Read More

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Proteomic Changes during the Dermal Toxicity Induced by Jellyfish Venom in HaCaT Human Keratinocyte.

Toxins (Basel) 2021 04 27;13(5). Epub 2021 Apr 27.

College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea.

Jellyfish venom is well known for its local skin toxicities and various lethal accidents. The main symptoms of local jellyfish envenomation include skin lesions, burning, prickling, stinging pain, red, brown, or purplish tracks on the skin, itching, and swelling, leading to dermonecrosis and scar formation. However, the molecular mechanism behind the action of jellyfish venom on human skin cells is rarely understood. Read More

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Viscoelastometric Testing to Assess Hemostasis of COVID-19: A Systematic Review.

J Clin Med 2021 Apr 16;10(8). Epub 2021 Apr 16.

Namur Thrombosis and Hemostasis Center (NTHC), CHU UCL Namur, Université Catholique de Louvain, 5530 Yvoir, Belgium.

Infection by SARS-CoV-2 is associated with a high risk of thrombosis. The laboratory documentation of hypercoagulability and impaired fibrinolysis remains a challenge. Our aim was to assess the potential usefulness of viscoelastometric testing (VET) to predict thrombotic events in COVID-19 patients according to the literature. Read More

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The Urokinase Receptor: A Multifunctional Receptor in Cancer Cell Biology. Therapeutic Implications.

Int J Mol Sci 2021 Apr 16;22(8). Epub 2021 Apr 16.

Department of Chemistry and Biology, University of Salerno, Fisciano, 84084 Salerno, Italy.

Proteolysis is a key event in several biological processes; proteolysis must be tightly controlled because its improper activation leads to dramatic consequences. Deregulation of proteolytic activity characterizes many pathological conditions, including cancer. The plasminogen activation (PA) system plays a key role in cancer; it includes the serine-protease urokinase-type plasminogen activator (uPA). Read More

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Plasminogen Activator Inhibitor-1 () Gene Polymorphisms Associated with Cardiovascular Risk Factors Involved in Cerebral Venous Sinus Thrombosis.

Metabolites 2021 Apr 23;11(5). Epub 2021 Apr 23.

Department of Neurology, Victor Babes University of Medicine and Pharmacy, 300041 Timisoara, Romania.

Cerebral venous sinus thrombosis (CVST), accounting for less than 1% of stroke cases, is characterized by various causes, heterogeneous clinical presentation and different outcome. The plasminogen activator inhibitor-1 () gene polymorphisms has been found to be associated with CVST. The aim of this retrospective study was to determine the potential association of polymorphisms and homocysteine levels with cardiovascular risk factors in a group of young patients with CVST. Read More

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Dietary Strawberries Improve Cardiometabolic Risks in Adults with Obesity and Elevated Serum LDL Cholesterol in a Randomized Controlled Crossover Trial.

Nutrients 2021 Apr 23;13(5). Epub 2021 Apr 23.

Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

Dietary berries, such as strawberries, are rich in bioactive compounds and have been shown to lower cardiometabolic risk. We examined the effects of two dietary achievable doses of strawberries on glycemic control and lipid profiles in obese adults with elevated serum LDL cholesterol (LDL-C). In this 14-week randomized controlled crossover study, participants were assigned to one of the three arms for four weeks separated by a one-week washout period: control powder, one serving (low dose: 13 g strawberry powder/day), or two-and-a -half servings (high dose: 32 g strawberry powder/day). Read More

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Plasminogen Activators in Neurovascular and Neurodegenerative Disorders.

Int J Mol Sci 2021 Apr 22;22(9). Epub 2021 Apr 22.

Neuropharmacology and Neurological Diseases Section, Yerkes National Primate Research Center, Atlanta, GA 30329, USA.

The neurovascular unit (NVU) is a dynamic structure assembled by endothelial cells surrounded by a basement membrane, pericytes, astrocytes, microglia and neurons. A carefully coordinated interplay between these cellular and non-cellular components is required to maintain normal neuronal function, and in line with these observations, a growing body of evidence has linked NVU dysfunction to neurodegeneration. Plasminogen activators catalyze the conversion of the zymogen plasminogen into the two-chain protease plasmin, which in turn triggers a plethora of physiological events including wound healing, angiogenesis, cell migration and inflammation. Read More

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Fibrinolytic System and Cancer: Diagnostic and Therapeutic Applications.

Int J Mol Sci 2021 Apr 22;22(9). Epub 2021 Apr 22.

Department of Medicine, McGill University, Montréal, QC H4A3J1, Canada.

Fibrinolysis is a crucial physiological process that helps to maintain a hemostatic balance by counteracting excessive thrombosis. The components of the fibrinolytic system are well established and are associated with a wide array of physiological and pathophysiological processes. The aberrant expression of several components, especially urokinase-type plasminogen activator (uPA), its cognate receptor uPAR, and plasminogen activator inhibitor-1 (PAI-1), has shown a direct correlation with increased tumor growth, invasiveness, and metastasis. Read More

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Plasmin and Plasminogen System in the Tumor Microenvironment: Implications for Cancer Diagnosis, Prognosis, and Therapy.

Cancers (Basel) 2021 Apr 12;13(8). Epub 2021 Apr 12.

Department of Pathology, Dalhousie University, Halifax, NS B3H 4R2, Canada.

The tumor microenvironment (TME) is now being widely accepted as the key contributor to a range of processes involved in cancer progression from tumor growth to metastasis and chemoresistance. The extracellular matrix (ECM) and the proteases that mediate the remodeling of the ECM form an integral part of the TME. Plasmin is a broad-spectrum, highly potent, serine protease whose activation from its precursor plasminogen is tightly regulated by the activators (uPA, uPAR, and tPA), the inhibitors (PAI-1, PAI-2), and plasminogen receptors. Read More

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A Novel Volume-Stable Collagen Matrix Induces Changes in the Behavior of Primary Human Oral Fibroblasts, Periodontal Ligament, and Endothelial Cells.

Int J Mol Sci 2021 Apr 14;22(8). Epub 2021 Apr 14.

Department of Periodontology, School of Dental Medicine, University of Bern, 3010 Bern, Switzerland.

The aim of the present study was to investigate the influence of a novel volume-stable collagen matrix (vCM) on early wound healing events including cellular migration and adhesion, protein adsorption and release, and the dynamics of the hemostatic system. For this purpose, we utilized transwell migration and crystal violet adhesion assays, ELISAs for quantification of adsorbed and released from the matrix growth factors, and qRT-PCR for quantification of gene expression in cells grown on the matrix. Our results demonstrated that primary human oral fibroblasts, periodontal ligament, and endothelial cells exhibited increased migration toward vCM compared to control cells that migrated in the absence of the matrix. Read More

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Reconciling the Biological and Transcriptional Variability of Hepatoblastoma with Its Mutational Uniformity.

Cancers (Basel) 2021 Apr 21;13(9). Epub 2021 Apr 21.

Division of Hematology/Oncology, UPMC Children's Hospital of Pittsburgh, Pittsburgh, PA 15224, USA.

Hepatoblastoma (HB), the most common childhood liver cancer, is associated with seven distinct histologic subtypes and variable degrees of clinical aggressiveness and presentation. Yet it is among the least genomically altered tumors known, with about half of HBs showing mutation and/or dysregulation of the Wnt/β-catenin and Hippo pathways. This raises the question of how this mutational simplicity can generate such biological and histologic complexity. Read More

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