505 results match your criteria plasmids trigger


Programmed Cell Death in Neurospora crassa Is Controlled by the Allorecognition Determinant rcd-1.

Genetics 2019 Dec;213(4):1387-1400

Plant and Microbial Biology Department, The University of California, Berkeley, California 94720.

Programmed cell death preventing heterokaryon formation in fungi has been proposed to represent a defense mechanism against conspecific genome exploitation and mycoviruses. Here, Daskalov et al. identified a novel genetic determinant of allorecognition. Read More

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December 2019

Programmed Cell Death in Neurospora crassa Is Controlled by the Allorecognition Determinant rcd-1.

Genetics 2019 Dec;213(4):1387-1400

Plant and Microbial Biology Department, The University of California, Berkeley, California 94720.

Programmed cell death preventing heterokaryon formation in fungi has been proposed to represent a defense mechanism against conspecific genome exploitation and mycoviruses. Here, Daskalov et al. identified a novel genetic determinant of allorecognition. Read More

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December 2019

Immune Protective Evaluation Elicited by DNA Vaccination With Dense Granules Proteins in Mice.

Front Vet Sci 2021 26;8:638067. Epub 2021 Feb 26.

Jiangsu Key Laboratory of Marine Biological Resources and Environment, Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, China.

, an obligate intracellular protozoan, is the major cause for neosporosis and brings serious economic losses to cattle breeding industries worldwide. After invasion, dense granules proteins are abundantly secreted and being important components of parasitophorous vacuole and intravacuolar network where survives and replicates. The aim of the present study was to evaluate the protective immunity induced by DNA vaccines with genes encoding dense granules proteins 1 (GRA1), GRA4, GRA9, GRA14, GRA17, and GRA23 against tachyzoites in BALB/C mice. Read More

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February 2021

IncC helper dependent plasmid-like replication of Salmonella Genomic Island 1.

Nucleic Acids Res 2021 01;49(2):832-846

Agricultural Biotechnology Institute, National Agricultural Research and Innovation Centre, Gödöllő H2100, Hungary.

The Salmonella genomic island 1 (SGI1) and its variants are mobilized by IncA and IncC conjugative plasmids. SGI1-family elements and their helper plasmids are effective transporters of multidrug resistance determinants. SGI1 exploits the transfer apparatus of the helper plasmid and hijacks its activator complex, AcaCD, to trigger the expression of several SGI1 genes. Read More

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January 2021

Resistance risk induced by quorum sensing inhibitors and their combined use with antibiotics: Mechanism and its relationship with toxicity.

Chemosphere 2021 Feb 1;265:129153. Epub 2020 Dec 1.

State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China; Shanghai Key Lab of Chemical Assessment and Sustainability, Shanghai, China; Post-doctoral Research Station, College of Civil Engineering, Tongji University, Shanghai, 200092, China. Electronic address:

The abuse of antibiotics has brought out serious bacterial resistance, which threatens the ecological environment and human health. Quorum sensing inhibitors (QSIs), as a new kind of potential antibiotic substitutes that are theoretically difficult to trigger bacterial resistance, are recommended to individually use or jointly use with traditional antibiotics. However, there are few studies on the resistance risk in the use of QSIs. Read More

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February 2021

Improving transgene expression and CRISPR-Cas9 efficiency with molecular engineering-based molecules.

Clin Transl Med 2020 Oct;10(6):e194

Guangdong Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Institute of Translational Medicine, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China.

As a novel and robust gene-editing tool, the Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-associated protein 9 (CRISPR-Cas9) system has revolutionized gene therapy. Plasmid vector delivery is the most commonly used method for integrating the CRISPR-Cas9 system into cells. However, such foreign cytosolic DNAs trigger an innate immune response (IIR) within cells, which can hinder gene editing by inhibiting transgene expression. Read More

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October 2020

Conditional guide RNA through two intermediate hairpins for programmable CRISPR/Cas9 function: building regulatory connections between endogenous RNA expressions.

Nucleic Acids Res 2020 11;48(20):11773-11784

MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, China.

A variety of nanodevices developed for nucleic acid computation provide great opportunities to construct versatile synthetic circuits for manipulation of gene expressions. In our study, by employing a two-hairpin mediated nucleic acid strand displacement as a processing joint for conditional guide RNA, we aim to build artificial connections between naturally occurring RNA expressions through programmable CRISPR/Cas9 function. This two-hairpin joint possesses a sequence-switching machinery, in which a random trigger strand can be processed to release an unconstrained sequence-independent strand and consequently activate the self-inhibitory guide RNA for conditional gene regulation. Read More

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November 2020

Chemistry of Class 1 CRISPR-Cas effectors: Binding, editing, and regulation.

J Biol Chem 2020 10 14;295(42):14473-14487. Epub 2020 Aug 14.

Department of Molecular and Cell Biology, University of California, Berkeley, California, USA

Among the multiple antiviral defense mechanisms found in prokaryotes, CRISPR-Cas systems stand out as the only known RNA-programmed pathways for detecting and destroying bacteriophages and plasmids. Class 1 CRISPR-Cas systems, the most widespread and diverse of these adaptive immune systems, use an RNA-guided multiprotein complex to find foreign nucleic acids and trigger their destruction. In this review, we describe how these multisubunit complexes target and cleave DNA and RNA and how regulatory molecules control their activities. Read More

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October 2020

A novel TSC1 frameshift mutation c.1550_1551del causes tuberous sclerosis complex by aberrant splicing and nonsense-mediated mRNA degradation (NMD) simultaneously in a Chinese family.

Mol Genet Genomic Med 2020 10 31;8(10):e1410. Epub 2020 Jul 31.

Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.

Background: Tuberous sclerosis complex (TSC), belongs to autosomal dominant genetic disorder, which affects multiple organ systems in the body, including the skin, brain, lungs, kidneys, liver, and eyes. Mutations in TSC1 or TSC2 was proved to be associated with these conditions.

Methods: Gene-panel Sequence of NGS was used to detect the mutation in a Chinese family. Read More

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October 2020

Molecular characterization of a fungal gasdermin-like protein.

Proc Natl Acad Sci U S A 2020 08 23;117(31):18600-18607. Epub 2020 Jul 23.

Plant and Microbial Biology Department, University of California, Berkeley, CA 94720;

Programmed cell death (PCD) in filamentous fungi prevents cytoplasmic mixing following fusion between conspecific genetically distinct individuals (allorecognition) and serves as a defense mechanism against mycoparasitism, genome exploitation, and deleterious cytoplasmic elements (i.e., senescence plasmids). Read More

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Smart pH-Regulated Switchable Nanoprobes for Photoelectrochemical Multiplex Detection of Antibiotic Resistance Genes.

Anal Chem 2020 08 6;92(16):11476-11483. Epub 2020 Aug 6.

Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China.

Antibiotic resistance, encoded via particular genes, has become a major global health threat and substantial burden on healthcare. Hence, the facile, low-cost, and precise detection of antibiotic resistance genes (ARGs) is crucial in the realm of human health and safety, especially multiplex sensing assays. Here, a smart pH-regulated switchable photoelectrochemical (PEC) bioassay has been created for ultrasensitive detection of two typical subtypes of penicillin resistance genes (target 1, labeled as T) and (target 2, labeled as T), whereby pH-responsive antimony tartrate (SbT) complex-grafted silica nanospheres are ingeniously adopted as signal DNA1 tags (labeled as S-SbT@SiONSs). Read More

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Rap-Phr Systems from Plasmids pAW63 and pHT8-1 Act Together To Regulate Sporulation in the Bacillus thuringiensis Serovar kurstaki HD73 Strain.

Appl Environ Microbiol 2020 09 1;86(18). Epub 2020 Sep 1.

Micalis Institute, INRAE, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France

is a Gram-positive spore-forming bacterium pathogenic to various insect species. This property is due to the Cry toxins encoded by plasmid genes and mostly produced during sporulation. contains a remarkable number of extrachromosomal DNA molecules and a great number of plasmid genes. Read More

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September 2020

Fungal senescence induced by the Neurospora sen mutation and mitochondrial plasmids - the contributions of Ramesh Maheshwari.

Int J Dev Biol 2020 ;64(1-2-3):29-34

Centre for DNA Fingerprinting and Diagnostics, Uppal, Hyderabad, India.

This article describes some of the research contributions made by Prof. Ramesh Maheshwari and his colleagues at the Indian Institute of Science, Bangalore. These include (1) the understanding of the Neurospora life cycle in agricultural (sugarcane) fields, (2) identification of Neurospora mutants that trigger vegetative spore development via microcycle conidiation, and (3) isolation of wild Neurospora strains in which the essential immortality of the fungal mycelia is subverted. Read More

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January 2020

Stress Can Induce Transcription of Toxin-Antitoxin Systems without Activating Toxin.

Mol Cell 2020 07 12;79(2):280-292.e8. Epub 2020 Jun 12.

Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address:

Toxin-antitoxin (TA) systems are ubiquitous genetic elements in bacterial genomes, but their functions are controversial. Although they are frequently postulated to regulate cell growth following stress, few null phenotypes for TA systems have been reported. Here, we show that TA transcript levels can increase substantially in response to stress, but toxin is not liberated. Read More

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The development of ghost vaccines trials.

Expert Rev Vaccines 2020 06 15;19(6):549-562. Epub 2020 Jun 15.

Morehouse School of Medicine , Atlanta, GA, USA.

Introduction: Bacterial ghosts are intact bacterial cell envelopes that are emptied of their content by gentle biological or chemical poring methods. Ghost techniques increase the safety of the killed vaccines, while maintaining their antigenicity due to mild preparation procedures. Moreover, ghost-platforms may express and/or carry several antigens or plasmid-DNA encoding for protein epitopes. Read More

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A Minimal Genetic Passkey to Unlock Many Legume Doors to Root Nodulation by Rhizobia.

Genes (Basel) 2020 05 7;11(5). Epub 2020 May 7.

University of Geneva, Sciences III, Department of Botany and Plant Biology, Microbiology Unit, 30 quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.

On legume crops, formation of developmentally mature nodules is a prerequisite to efficient nitrogen fixation by populations of rhizobial bacteroids established inside nodule cells. Development of root nodules and concomitant microbial colonisation of plant cells are constrained by sets of recognition signals exchanged by infecting rhizobia and their legume hosts, with much of the specificity of symbiotic interactions being determined by the flavonoid cocktails released by legume roots and the strain-specific nodulation factors (NFs) secreted by rhizobia. Hence, much of strain NGR234 symbiotic promiscuity was thought to stem from a family of >80 structurally diverse NFs and associated nodulation keys in the form of secreted effector proteins and rhamnose-rich surface polysaccharides. Read More

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Development of an epigenetic tetracycline sensor system based on DNA methylation.

PLoS One 2020 7;15(5):e0232701. Epub 2020 May 7.

Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart, Stuttgart, Germany.

Bacterial live cell sensors are potentially powerful tools for the detection of environmental toxins. In this work, we have established and validated a flow cytometry readout for an existing bacterial arabinose sensor system with DNA methylation based memory function (Maier et al., 2017, Nat. Read More

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CRISPR-Cas12a: Functional overview and applications.

Biomed J 2020 02 5;43(1):8-17. Epub 2020 Feb 5.

Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of Copenhagen, Copenhagen, Denmark. Electronic address:

Prokaryotes have developed an adaptive immune system called Clustered regularly interspaced short palindromic repeats (CRISPR) to combat attacks by foreign mobile genetic elements (MGEs) such as plasmids and phages. In the past decade, the widely characterized CRISPR-Cas9 enzyme has been redesigned to trigger a genome editing revolution. Class II type V CRISPR-Cas12a is a new RNA guided endonuclease that has been recently harnessed as an alternative genome editing tool, which is emerging as a powerful molecular scissor to consider in the genome editing application landscape. Read More

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February 2020

FMRpolyG accumulates in FMR1 premutation granulosa cells.

J Ovarian Res 2020 Feb 26;13(1):22. Epub 2020 Feb 26.

Sackler Faculty of Medicine, Tel-Aviv University, Tel Aviv, Israel.

Background: Fragile X premutation (Amplification of CGG number 55-200) is associated with increased risk for fragile X-Associated Premature Ovarian Insufficiency (FXPOI) in females and fragile X-associated tremor/ataxia syndrome (FXTAS) predominantly in males. Recently, it has been shown that CGG repeats trigger repeat associated non-AUG initiated translation (RAN) of a cryptic polyglycine-containing protein, FMRpolyG. This protein accumulates in ubiquitin-positive inclusions in neuronal brain cells of FXTAS patients and may lead to protein-mediated neurodegeneration. Read More

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February 2020

TRIM32 triggers β-catenin signaling through ubiquitylation of AXIN1 to promote inflammatory factor-induced apoptosis of rat nucleus pulposus cells.

Am J Physiol Cell Physiol 2020 03 22;318(3):C695-C703. Epub 2020 Jan 22.

Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai, China.

The dysregulation of ubiquitin ligase is the cause of many human diseases. Tripartite motif protein 32 (TRIM32) is an E3 ubiquitin ligase whose role in nucleus pulposus (NP) cell apoptosis is unclear. The expression of TRIM family protein and β-catenin in 40 NP tissue samples was detected by RT-PCR. Read More

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CRYAB inhibits migration and invasion of bladder cancer cells through the PI3K/AKT and ERK pathways.

Jpn J Clin Oncol 2020 Mar;50(3):254-260

Department of Urology, The First Affiliated Hospital of Anhui Medical University and Institute of Urology, AHMU, Hefei, China.

Background: Bladder cancer is a common malignancy characterized by a high recurrence rate and the development of drug resistance. Frequent mutations and gene expression alterations in the PI3K/AKT and mitogen-activated protein kinase-ERK pathways lead to deregulated cell growth and the acquisition of invasive properties, which facilitates tumour progression and confers resistance to chemotherapy. Therefore, identification of the underlying mechanisms that trigger the activation of these signalling pathways and control the invasive phenotype of tumour cells is of urgent need. Read More

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Arginine- but not alanine-rich carboxy-termini trigger nuclear translocation of mutant keratin 10 in ichthyosis with confetti.

J Cell Mol Med 2019 12 22;23(12):8442-8452. Epub 2019 Oct 22.

Department of Biomedicine, University Hospital Basel and University of Basel, Basel, Switzerland.

Ichthyosis with confetti (IWC) is a genodermatosis associated with dominant-negative variants in keratin 10 (KRT10) or keratin 1 (KRT1). These frameshift variants result in extended aberrant proteins, localized to the nucleus rather than the cytoplasm. This mislocalization is thought to occur as a result of the altered carboxy (C)-terminus, from poly-glycine to either a poly-arginine or -alanine tail. Read More

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December 2019

Programmed Cell Death in Is Controlled by the Allorecognition Determinant .

Genetics 2019 12 21;213(4):1387-1400. Epub 2019 Oct 21.

Plant and Microbial Biology Department, The University of California, Berkeley, California 94720

Nonself recognition following cell fusion between genetically distinct individuals of the same species in filamentous fungi often results in a programmed cell death (PCD) reaction, where the heterokaryotic fusion cell is compartmentalized and rapidly killed. The allorecognition process plays a key role as a defense mechanism that restricts genome exploitation, resource plundering, and the spread of deleterious senescence plasmids and mycoviruses. Although a number of incompatibility systems have been described that function in mature hyphae, less is known about the PCD pathways in asexual spores, which represent the main infectious unit in various human and plant fungal pathogens. Read More

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December 2019

A Bacterial Effector Mimics a Host HSP90 Client to Undermine Immunity.

Cell 2019 09 12;179(1):205-218.e21. Epub 2019 Sep 12.

Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address:

The molecular chaperone HSP90 facilitates the folding of several client proteins, including innate immune receptors and protein kinases. HSP90 is an essential component of plant and animal immunity, yet pathogenic strategies that directly target the chaperone have not been described. Here, we identify the HopBF1 family of bacterial effectors as eukaryotic-specific HSP90 protein kinases. Read More

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September 2019

Presenilin 1 Regulates [Ca]i and Mitochondria/ER Interaction in Cultured Rat Hippocampal Neurons.

Oxid Med Cell Longev 2019 28;2019:7284967. Epub 2019 Jul 28.

Department of Neurobiology, The Weizmann Institute, Rehovot 76100, Israel.

Mutations in the presenilin 1 (PS1) gene are a major trigger of familial Alzheimer's disease (AD), yet the mechanisms affected by mutated PS1 causing cognitive decline are not yet elucidated. In the present study, we compared rat hippocampal neurons in culture, transfected with PS1 or with mutant (M146V) PS1 (mPS1) plasmids in several neuronal functions. Initially, we confirmed earlier observations that mPS1-expressing neurons are endowed with fewer mature "mushroom" spines and more filopodial immature protrusions. Read More

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January 2020

A Dengue Virus Type 2 (DENV-2) NS4B-Interacting Host Factor, SERP1, Reduces DENV-2 Production by Suppressing Viral RNA Replication.

Viruses 2019 08 27;11(9). Epub 2019 Aug 27.

Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli 35053, Taiwan.

Host cells infected with dengue virus (DENV) often trigger endoplasmic reticulum (ER) stress, a key process that allows viral reproduction, without killing the host cells until the late stage of the virus life-cycle. However, little is known regarding which DENV viral proteins interact with the ER machinery to support viral replication. In this study, we identified and characterized a novel host factor, stress-associated ER protein 1 (SERP1), which interacts with the DENV type 2 (DENV-2) NS4B protein by several assays, for example, yeast two-hybrid, subcellular localization, NanoBiT complementation, and co-immunoprecipitation. Read More

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PPAR Receptors Expressed from Vectors Containing CMV Promoter Can Enhance Self-Transcription in the Presence of Fatty Acids from CLA-Enriched Egg Yolks-A Novel Method for Studies of PPAR Ligands.

Nutr Cancer 2020 12;72(5):892-902. Epub 2019 Aug 12.

Department of Human Nutrition, Faculty of Food Technology, University of Agriculture, Krakow, Poland.

PPAR receptors are ligand-dependent transcription factors activated in response to various small lipophilic ligands controlling the expression of different genes involved in cellular differentiation, development, metabolism, and tumorigenesis. Unexpectedly, our previous studies have shown that single plasmid-based expression of PPARs under the control of CMV promoter/enhancer was significantly elevated in the presence of PPAR agonists. Here we show that the PPAR reporters controlled by the CMV promoter/enhancer, that was shown to contain three internal non-canonical PPRE elements, can be used as a fast screening system for more effective PPAR ligands. Read More

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Defensive Function of Transposable Elements in Bacteria.

ACS Synth Biol 2019 09 19;8(9):2141-2151. Epub 2019 Aug 19.

Department of Engineering Science , University of Oxford , Parks Road , OX1 3PJ Oxford , United Kingdom.

It has been widely debated whether transposable elements have a positive or a negative effect on their host cells. This study demonstrated that transposable elements, specifically insertion sequences (ISs), can adopt a defensive role in . In three different strains (S17, DH5α, and Nissle 1917), IS1 and IS10 rapidly disrupted the gene (encoding I-CeuI endonuclease) on the plasmid pLO11-ICeuI as early as the first generation, despite the gene-circuit being under control of an arabinose promoter. Read More

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September 2019

pUC18-CpG stimulates RAW 264.7 via TBK1-mediated pathway and presents adjuvanticity in mice.

Pol J Vet Sci 2019 Jun;22(2):195-201

Department of Veterinary Medicine, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, 100193 Beijing, China.

Phosphorothioate CpG oligodeoxynucleotides (ODN) are reported to be recognized by the membrane-bound TLR9 and trigger the MyD88-dependent up-regulation of Type I interferons and pro-inflammatory cytokines. Whether plasmids containing multiple CpG motifs stimulate the same signaling pathway is yet to be determined. The present results show that the CpG motifs enrich plasmid pUC18-CpG stimulates RAW 264. Read More

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Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy.

J Vis Exp 2019 06 7(148). Epub 2019 Jun 7.

Vollum Institute, Oregon Health & Science University;

Neuromodulation exerts powerful control over brain function. Dysfunction of neuromodulatory systems results in neurological and psychiatric disorders. Despite their importance, technologies for tracking neuromodulatory events with cellular resolution are just beginning to emerge. Read More

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