26,253 results match your criteria placental expression

RNA Network Interactions During Differentiation of Human Trophoblasts.

Front Cell Dev Biol 2021 3;9:677981. Epub 2021 Jun 3.

Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States.

In the human placenta, two trophoblast cell layers separate the maternal blood from the villous basement membrane and fetal capillary endothelial cells. The inner layer, which is complete early in pregnancy and later becomes discontinuous, comprises the proliferative mononuclear cytotrophoblasts, which fuse together and differentiate to form the outer layer of multinucleated syncytiotrophoblasts. Because the syncytiotrophoblasts are responsible for key maternal-fetal exchange functions, tight regulation of this differentiation process is critical for the proper development and the functional role of the placenta. Read More

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Combined detection of ACL and β2-GP1 in diagnosis of adverse pregnancy.

Am J Transl Res 2021 15;13(5):5653-5658. Epub 2021 May 15.

Department of Obstetrics, Hengshui People's Hospital Hengshui, China.

Objective: To illustrate the significance of combined detection of anti cardiolipin (ACL) and anti-β2-glycoprotein 1 (β2-GP1) in the diagnosis of adverse pregnancy.

Methods: 60 postnatal women with adverse pregnancy presented to our hospital from March 2019 to March 2020 were enrolled as the experimental group, and 60 postnatal women with normal delivery in the same period were selected as the control group. The levels of ACL, β2-GP1 were measured to analyze the significance of the integrated detection in the diagnosis of adverse pregnancy. Read More

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miR-335 targets CRIM1 to promote the proliferation and inhibit the apoptosis of placental trophoblast cells in preeclamptic rats.

Am J Transl Res 2021 15;13(5):4676-4685. Epub 2021 May 15.

Department of Obstetrics, Liuzhou Worker's Hospital, The Forth Affiliated Hospital of Guangxi Medical University Liuzhou, Guangxi Zhuang Autonomous Region, China.

Objective: To investigate the role of miRNA-335 on the proliferation and apoptosis of placental trophoblast cells in preeclamptic rats and its potential mechanism.

Methods: Placental trophoblast cells were isolated from preeclamptic model rats and normal ones. Trophoblast cells from the model group were divided into six groups for transfection: the blank (empty vector) group, the NC (negative control) group, the miRNA-335 mimic group, the miRNA-335 inhibitor group, the CRIM1 (overexpressed recombinant plasmid) group, and the miRNA-335 inhibitor + CRIM1 group. Read More

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STAT3-mediated effects of methyltransferase inhibitor 5-aza-2'-deoxycytidine on preeclampsia.

Am J Transl Res 2021 15;13(5):4103-4119. Epub 2021 May 15.

The Third School of Clinical Medicine, Southern Medical University Guangzhou 510800, China.

Objective: To investigate the effects of 5-aza-2'-deoxycytidine (5-AZA-DC) on preeclampsia (PE) and functional mechanisms dependent on STAT3.

Materials And Methods: Trophoblastic cells (HTR8/Svneo, JEG-3, JAR and BeWo) were used to constructed STAT3-overexpressing or -silenced cells. qRT-PCR, Western blot, and FISH were used to detect mRNA and protein expression. Read More

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Placenta inflammation is closely associated with gestational diabetes mellitus.

Am J Transl Res 2021 15;13(5):4068-4079. Epub 2021 May 15.

Department of Pediatric, Shengjing Hospital of China Medical University Shenyang, China.

Objective: To investigate the potential role of placenta inflammation in gestational diabetes mellitus (GDM) and construct a model for the diagnosis of GDM.

Methods: In this study, transcriptome-wide profiling datasets, GSE70493 and GSE128381 were downloaded from Gene Expression Omnibus (GEO) database. Significant immune-related genes were identified separately to be the biomarkers for the diagnosis of GDM by using random forest model (RF), support vector machine model (SVM), and generalized linear model (GLM). Read More

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Inefficient Placental Virus Replication and Absence of Neonatal Cell-Specific Immunity Upon Sars-CoV-2 Infection During Pregnancy.

Front Immunol 2021 3;12:698578. Epub 2021 Jun 3.

Department of Obstetrics and Fetal Medicine, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany.

Pregnant women have been carefully observed during the COVID-19 pandemic, as the pregnancy-specific immune adaptation is known to increase the risk for infections. Recent evidence indicates that even though most pregnant have a mild or asymptomatic course, a severe course of COVID-19 and a higher risk of progression to diseases have also been described, along with a heightened risk for pregnancy complications. Yet, vertical transmission of the virus is rare and the possibility of placental SARS-CoV-2 infection as a prerequisite for vertical transmission requires further studies. Read More

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FKBPL and SIRT-1 Are Downregulated by Diabetes in Pregnancy Impacting on Angiogenesis and Endothelial Function.

Front Endocrinol (Lausanne) 2021 2;12:650328. Epub 2021 Jun 2.

The Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Northern Ireland, United Kingdom.

Diabetes in pregnancy is associated with adverse pregnancy outcomes including preterm birth. Although the mechanisms leading to these pregnancy complications are still poorly understood, aberrant angiogenesis and endothelial dysfunction play a key role. FKBPL and SIRT-1 are critical regulators of angiogenesis, however, their roles in pregnancies affected by diabetes have not been examined before in detail. Read More

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The roles of free iron, heme, hemoglobin, and the scavenger proteins hemopexin and alpha-1-microglobulin in preeclampsia and fetal growth restriction.

J Intern Med 2021 Jun 19. Epub 2021 Jun 19.

Division of Obstetrics and Gynecology, Department of Clinical Sciences Lund, Lund University, Lund, Sweden.

Background: Preeclampsia (PE) is a complex pregnancy syndrome characterized by maternal hypertension and organ damage after 20 weeks of gestation and is associated with increased risk of cardiovascular disease later in life. Extracellular hemoglobin (Hb) and its metabolites heme and iron are highly toxic molecules and several defense mechanisms have evolved to protect the tissue.

Objectives: We will discuss the roles of free iron, heme, Hb, and the scavenger proteins hemopexin and A1M in pregnancies complicated by PE and FGR. Read More

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Coordination of two enhancers drives expression of olfactory trace amine-associated receptors.

Nat Commun 2021 06 18;12(1):3798. Epub 2021 Jun 18.

Center for Brain Science of Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Olfactory sensory neurons (OSNs) are functionally defined by their expression of a unique odorant receptor (OR). Mechanisms underlying singular OR expression are well studied, and involve a massive cross-chromosomal enhancer interaction network. Trace amine-associated receptors (TAARs) form a distinct family of olfactory receptors, and here we find that mechanisms regulating Taar gene choice display many unique features. Read More

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Tissue Transglutaminase Impairs HTR-8/SVneo Trophoblast Cell Invasion via the PI3K/AKT Signaling Pathway.

Gynecol Obstet Invest 2021 Jun 17:1-9. Epub 2021 Jun 17.

Department of Obstetrics, Guangzhou Medical University Affiliated Guangzhou Women and Children's Medical Center, Guangzhou, China.

Objectives: The pathogenesis of preeclampsia (PE) is associated with impaired trophoblast invasion, which results in placental insufficiency. Our earlier studies demonstrated that tissue transglutaminase (tTG) is highly expressed in human PE serum. However, whether tTG participates in trophoblast invasion remains unclear. Read More

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Late onset intra-uterine growth restriction and HHV-6 infection: a pilot study.

J Med Virol 2021 Jun 17. Epub 2021 Jun 17.

Department of Medical Sciences, Obstetric and Gynecological Clinic, University of Ferrara (Italy).

Background: Late onset intra-uterine growth restriction (IUGR) refers to impaired growth and development of the fetus, characterized by placental morphological abnormalities that affect the fetus supply of nutrients. Human Leukocyte Antigen-G (HLA-G) is physiologically expressed during pregnancy, but in normal placenta decreased during the last weeks of gestation possibly inducing childbirth. Several viruses involved in congenital infection, such as herpesviruses, exploit HLA-G expression as an immune-escape mechanism. Read More

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Expression of the Carbohydrate Lewis Antigen, Sialyl Lewis A, Sialyl Lewis X, Lewis X, and Lewis Y in the Placental Villi of Patients With Unexplained Miscarriages.

Front Immunol 2021 31;12:679424. Epub 2021 May 31.

Department of Obstetrics and Gynaecology, University Hospital LMU Munich, Munich, Germany.

Background: Lewis antigens such as Sialyl Lewis A (sLeA), Sialyl Lewis X (sLeX), Lewis X (LeX), and Lewis Y (LeY) are a class of carbohydrate molecules that are known to mediate adhesion between tumor cells and endothelium by interacting with its selectin ligands. However, their potential role in miscarriage remains enigmatic. This study aims to analyze the expression pattern of sLeA, sLeX, LeX, and LeY in the placental villi tissue of patients with a medical history of unexplained miscarriages. Read More

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Presence of surfactant proteins in the uteri and placentae of pregnant mares.

J Vet Med Sci 2021 Jun 15. Epub 2021 Jun 15.

Obihiro University of Agriculture and Veterinary Medicine.

Immunohistochemical investigations of the expression of surfactant protein A (SP-A) and surfactant protein D (SP-D) in the uterine and placental tissues of 13 pregnant mares were performed using anti-horse monoclonal primary antibodies. Strong positive reactions for both SP-A and SP-D were observed in the trophoblasts in the microcotyledons of the placentae at 182 to 314 days of gestation; in uterine glandular epithelial cells, faint-to-weak reactions were observed during gestation. This study describes, for the first time, the changes in the SP-A and SP-D expression levels in the endometrium of mares during gestation; the SP-A and SP-D expression levels increased after the second trimester of gestation. Read More

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Effects of aspirin, vitamin D3, and progesterone on pregnancy outcomes in an autoimmune recurrent spontaneous abortion model.

Braz J Med Biol Res 2021 14;54(9):e9570. Epub 2021 Jun 14.

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.

High proportions of placental lymphocytes expressing DX5+/CD25+/FOXP3+/CD45+/CD4+ are beneficial to maintain immune tolerance and improve pregnancy outcomes. This study aimed to compare and evaluate the therapeutic effects of aspirin, vitamin D3 (VitD3), and progesterone on the autoimmune recurrent spontaneous abortion (RSA) model. The autoimmune RSA mouse model was constructed, and the embryo loss rate was calculated for each group. Read More

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USP22 regulates the formation and function of placental vasculature during the development of fetal growth restriction.

Placenta 2021 Jun 9;111:19-25. Epub 2021 Jun 9.

Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, China. Electronic address:

Introduction: Fetal growth restriction (FGR) is a common obstetric complication that can lead to a variety of adverse perinatal outcomes and is associated with chronic diseases in adulthood. Since ubiquitin-specific protease 22 (USP22) is closely related to cell growth, differentiation and proliferation, we aimed to investigate the role of USP22 in FGR development.

Methods: USP22 expression was detected in the placentas of eight normal and eight pregnant women with FGR. Read More

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Maternal gestational mercury exposure in relation to cord blood T cell alterations and placental gene expression signatures.

Environ Res 2021 Jun 12;201:111385. Epub 2021 Jun 12.

Department of Epidemiology, Geisel School of Medicine and the Children's Environmental Health and Disease Prevention Research Center at Dartmouth; Hanover, NH, USA.

The immunotoxic impacts of mercury during early life is poorly understood. We investigated the associations between gestational mercury exposure and frequency of cord blood T cells as well as placental gene expression. Frequency of natural Treg cells was positively associated with prenatal and postpartum mercury toenail concentrations. Read More

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Loss of Selenoprotein Iodothyronine Deiodinase 3 Expression Correlates with Progression of Complete Hydatidiform Mole to Gestational Trophoblastic Neoplasia.

Reprod Sci 2021 Jun 15. Epub 2021 Jun 15.

Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Brigham and Women's Hospital, 75 Francis St., Boston, MA, 02115, USA.

To investigate if differences in imprinting at tropho-microRNA (miRNA) genomic clusters can distinguish between pre-gestational trophoblastic neoplasia cases (pre-GTN) and benign complete hydatidiform mole (CHM) cases at the time of initial uterine evacuation. miRNA sequencing was performed on frozen tissue from 39 CHM cases including 9 GTN cases. DIO3, DLK1, RTL1, and MEG 3 mRNA levels were assessed by qRT-PCR. Read More

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Thrombomodulin promotes placental function by up-regulating placental growth factor via inhibition of high-mobility-group box 1 and hypoxia-inducible factor 1α.

Placenta 2021 Jun 11;111:1-9. Epub 2021 Jun 11.

Department of Obstetrics and Gynecology, Faculty of Medicine, The University of Tokyo, Tokyo, Japan.

Introduction: Pregnancy is a state of maternal systemic stress due to inflammation and hypoxic reactions originating from the utero-placental unit. Maternal tolerance to these stresses is a key for successful outcomes. Thrombomodulin (TM), a glycoprotein expressed on cell surface, regulates local inflammatory pathways by inhibiting proinflammatory factor, High-mobility-group box1(HMGB1). Read More

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Proteomic Analysis Reveals That Placenta-Specific Protein 9 Inhibits Proliferation and Stimulates Motility of Human Bronchial Epithelial Cells.

Front Oncol 2021 28;11:628480. Epub 2021 May 28.

Institute for Medical Biology and Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China, College of Life Sciences, South-Central University for Nationalities, Wuhan, China.

Placenta-specific protein 9 (PLAC9) is a putative secretory protein that was initially identified in the placenta and is involved in cell proliferation and motility. Bioinformatics analyses revealed that PLAC9 is repressed in lung cancers (LCs), especially lung adenocarcinomas, compared to that in the paired adjacent normal tissues, indicating that PLAC9 might be involved in the pathogenesis of pulmonary diseases. To investigate the potential role of PLAC9 in the abnormal reprogramming of airway epithelial cells (AECs), a key cause of pulmonary diseases, we constructed a stable PLAC9-overexpressing human bronchial epithelial cell line (16HBE-GFP-). Read More

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Novel insights for the role of nitric oxide in placental vascular function during and beyond pregnancy.

J Cell Physiol 2021 Jun 14. Epub 2021 Jun 14.

Instituto de Ciencias de la Salud, Universidad de O'Higgins, Rancagua, Chile.

More than 30 years have passed since endothelial nitric oxide synthesis was described using the umbilical artery and vein endothelium. That seminal report set the cornerstone for unveiling the molecular aspects of endothelial function. In parallel, the understanding of placental physiology has gained growing interest, due to its crucial role in intrauterine development, with considerable long-term health consequences. Read More

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Global proteomic analyses of human cytotrophoblast differentiation/invasion.

Development 2021 Jun 14. Epub 2021 Jun 14.

Center for Reproductive Sciences, University of California, San Francisco, California 94143, USA.

During human pregnancy, cytotrophoblasts (CTBs) from the placenta differentiate into specialized subpopulations that play critical roles in proper fetal growth and development. A subset of these CTBs differentiate along an invasive pathway, penetrating the decidua and anchoring the placenta to the uterus. A critical hurdle in pregnancy is the ability of these cells to migrate, invade, and remodel spiral arteries, ensuring adequate blood flow to nourish the developing fetus. Read More

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Melatonin-MT1 signal is essential for endometrial decidualization.

Reproduction 2021 Jun 1. Epub 2021 Jun 1.

M Du, Gyn/Obs Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Deficient decidualization of endometrial stromal cells (ESCs) can cause adverse pregnancy outcomes including miscarriage, intrauterine growth restriction and pre-eclampsia. Decidualization is regulated by multiple factors such as hormones and circadian genes. Melatonin, a circadian-controlled hormone, is reported to be important for various reproductive process, including oocyte maturation and placenta development. Read More

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The Placental Ferroxidase Zyklopen Is Not Essential for Iron Transport to the Fetus in Mice.

J Nutr 2021 Jun 10. Epub 2021 Jun 10.

Molecular Nutrition Laboratory, QIMR Berghofer Medical Research Institute, Herston, Australia.

Background: The ferroxidase zyklopen (Zp) has been implicated in the placental transfer of iron to the fetus. However, the evidence for this is largely circumstantial.

Objectives: This study aimed to determine whether Zp is essential for placental iron transfer. Read More

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Placental chorionic plate-derived mesenchymal stem cells ameliorate severe acute pancreatitis by regulating macrophage polarization via secreting TSG-6.

Stem Cell Res Ther 2021 Jun 10;12(1):337. Epub 2021 Jun 10.

Department of General Surgery & Pancreatic Injury and Repair Key Laboratory of Sichuan Province, The General Hospital of Western Theater Command, Chengdu, 610083, China.

Background: Mesenchymal stem cells (MSCs) hold promising potential to treat systemic inflammatory diseases including severe acute pancreatitis (SAP). In our previous study, placental chorionic plate-derived MSCs (CP-MSCs) were found to possess superior immunoregulatory capability. However, the therapeutic efficacy of CP-MSCs on SAP and their underlying mechanism remain unclear. Read More

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Novel promising reproductive and metabolic effects of Cicer arietinum L. extract on letrozole induced polycystic ovary syndrome in rat model.

J Ethnopharmacol 2021 Jun 7;278:114318. Epub 2021 Jun 7.

Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt. Electronic address:

Ethnopharmacological Relevance: Chickpea was used in both greek and indian traditional medicine for hormonal related conditions as menstrual induction, acceleration of parturation, treatment of retained placenta and stimulation of lactation. Chickpea (Cicer arietinum) sprout isoflavone isolates exhibited reasonable estrogenic activities. Isoflavones, a subtype of phytoestrogens, are plant derivatives with moderate estrogenic activity that tend to have protective effects on hormonal and metabolic abnormalities of women with polycystic ovary syndrome (PCOS). Read More

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COVID-19 during Pregnancy: Clinical and In Vitro Evidence against Placenta Infection at Term by SARS-CoV-2.

Am J Pathol 2021 Jun 7. Epub 2021 Jun 7.

Pole of Obstetrics, Institut de Recherche Expérimentale et Clinique (IREC), Université catholique de Louvain (UCLouvain), Brussels, Belgium; Division of Obstetrics, Saint-Luc University Hospital, Brussels, Belgium.

Despite occasional reports of SARS-CoV-2 vertical transmission during pregnancy, the question of placental infection and its consequences for the newborn remain questionable. Here, we analyzed the placentas of 31 COVID-19-positive mothers by RT-PCR, immunohistochemistry and in situ hybridization. We only detected one case of placental infection, which was associated with intrauterine demise of the fetus. Read More

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LncRNA SNHG16 regulates trophoblast functions by the miR-218-5p/LASP1 axis.

J Mol Histol 2021 Jun 10. Epub 2021 Jun 10.

Department of Obstetrics, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, No. 1 Huanghe West Road, Huaiyin District, Huaian , 223300, Jiangsu, China.

Altered placental development and function lead to placental diseases such as preeclampsia (PE) which is mainly characterized by insufficient trophoblast invasion and abnormally invasive placenta disorders. Long noncoding RNAs (lncRNAs) are widely reported to function as crucial players in the pathogenesis of PE. The present investigation clarified the role of lncRNA small nucleolar RNA host gene 16 (SNHG16) in PE. Read More

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Placenta-specific protein 1 promotes cell proliferation via the AKT/GSK-3β/cyclin D1 signaling pathway in gastric cancer.

IUBMB Life 2021 Jun 10. Epub 2021 Jun 10.

Division of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, PR China.

Gastric cancer is a malignant tumor with a poor prognosis. Therefore, it is important to search for molecules that play a vital role in the development, diagnosis, and treatment of this disease. Placenta-specific 1 (PLAC1) is one of the cancer-testis antigens; it plays an important role in both placental development and tumorigenesis. Read More

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Long intergenic noncoding RNA 00473 promoting migration and invasion of trophoblastic cell line HTR-8/SVneo via regulating miR-424-5p-mediated wnt3a/β-catenin signaling pathway.

J Obstet Gynaecol Res 2021 Jun 9. Epub 2021 Jun 9.

Department of Obstetrics, Ningbo First Hospital, Ningbo, China.

Background: Preeclampsia (PE) is a serious obstetric complication. Recent studies point out that the functions of long intergenic noncoding RNA 00473 (linc00473), miR-424-5p, and Wnt/β-catenin signaling pathway were involved in the invasion and migration of extravillous trophoblast. Here, we investigated the role and mechanism of linc00473 in HTR-8/SVneo trophoblastic cell line and its role in PE. Read More

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Effect of IL-15-Mediating IFN-γ on HTR-8/SVneo Cells and a Preeclampsia Mouse Model Induced by Lipopolysaccharides.

Xiao-Lu Wang

Gynecol Obstet Invest 2021 Jun 9:1-10. Epub 2021 Jun 9.

Department of Obstetrics, Yantai Yantaishan Hospital, Yantai, China.

Objective: Determine the effect of interleukin (IL)-15 on HTR-8/SVneo cells and a preeclampsia (PE) mouse model induced by LPS.

Methods: Transwell and Annexin-V-FITC/PI assays were performed in HTR-8/SVneo cells transfected with IL-15 activation plasmid/siRNA prior to LPS treatment. Additionally, pregnant mice were injected with LPS and IL-15 siRNA followed by measurement of systolic blood pressure (SBP), urine protein, and serum NO. Read More

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