1,225,729 results match your criteria pkcη activation

Blood neutrophils from children with COVID-19 exhibit both inflammatory and anti-inflammatory markers.

EBioMedicine 2021 May 9;67:103357. Epub 2021 May 9.

División Infectología, Hospital General de Agudos Dr. Juan A. Fernández, Av. Cerviño 3356, CABA C1425, Argentina.

Background: Perhaps reflecting that children with COVID-19 rarely exhibit severe respiratory symptoms and often remain asymptomatic, little attention has been paid to explore the immune response in pediatric COVID-19. Here, we analyzed the phenotype and function of circulating neutrophils from children with COVID-19.

Methods: An observational study including 182 children with COVID-19, 21 children with multisystem inflammatory syndrome (MIS-C), and 40 healthy children was performed in Buenos Aires, Argentina. Read More

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Soybean residue based biochar prepared by ball milling assisted alkali activation to activate peroxydisulfate for the degradation of tetracycline.

J Colloid Interface Sci 2021 Apr 19;599:631-641. Epub 2021 Apr 19.

College of Environmental Science and Engineering, Hunan University, Lushan South Road, Yuelu District, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Lushan South Road, Yuelu District, Changsha 410082, PR China. Electronic address:

The advanced oxidation process (AOPs) has caused great concern in recent years. Among them, biochar has been widely studied as a catalyst for advanced oxidation process because of its low price and low environmental risk. In this study, a novel ball milling assisted KOH activation biochar (MKBC) was prepared and applied in peroxydisulfate (PDS) activation to degrade tetracycline hydrochloride (TC-H). Read More

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Structure-based modification of pyrazolone derivatives to inhibit mTORC1 by targeting the leucyl-tRNA synthetase-RagD interaction.

Bioorg Chem 2021 Apr 20;112:104907. Epub 2021 Apr 20.

Department of Integrated OMICS for Biomedical Sciences (WCU Program), Yonsei University, Seoul 03722, Republic of Korea; Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea; Department of pharmacy, College of Pharmacy, Yonsei University, Incheon 21983, Republic of Korea. Electronic address:

The enzyme leucyl-tRNA synthetase (LRS) and the amino acid leucine regulate the mechanistic target of rapamycin (mTOR) signaling pathway. Leucine-dependent mTORC1 activation depends on GTPase activating protein events mediated by LRS. In a prior study, compound BC-LI-0186 was discovered and shown to interfere with the mTORC1 signaling pathway by inhibiting the LRS-RagD interaction. Read More

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Ventral striatal activation during reward differs between major depression with and without impaired mood reactivity.

Psychiatry Res Neuroimaging 2021 May 2;313:111298. Epub 2021 May 2.

Department of Psychology, Florida State University, 1107 West Call St, Tallahassee, FL 32304, United States.

Background: Recent efforts to classify subtypes of major depressive disorder marked by different psychophysiological indicators have identified blunted reward-related brain activation in gambling tasks as a characteristic linked specifically to depressed participants with impaired mood reactivity.

Methods: The current study compared individuals diagnosed with current depressive disorder (n = 26) with healthy controls (n = 24) regarding brain responses to gain and loss trials in an fMRI version of the "Doors" choice-feedback task. Study aims were to examine reward-related brain activation in relation to depression, depressive subtypes, and course of depression. Read More

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Multipath elimination of bisphenol A over bifunctional polymeric carbon nitride/biochar hybrids in the presence of persulfate and visible light.

J Hazard Mater 2021 May 5;417:126008. Epub 2021 May 5.

State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.

Polymeric carbon nitride (PCN) has become a star material either in photocatalysis or in persulfate (PS) activation. In this work, we synthesized bifunctional biochar (BC)-doped PCN through a facile one-pot thermal treatment process. The PCN/BC hybrid (CNBC) with an optimized proportion could not only activate PS directly, but also possessed improved optical properties. Read More

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Meta-analytic exploration of the joint factors of the Brief Psychiatric Rating Scale - Expanded (BPRS-E) and the positive and negative symptoms scales (PANSS).

J Psychiatr Res 2021 Apr 23;138:519-527. Epub 2021 Apr 23.

Department of Human Sciences, Lumsa University, Rome, Italy.

Previous meta-analyses of the BPRS-E and PANSS were combined to analyze the joint factors across 77 factor analyses from 68 studies of 32,896 individuals. The BPRS-E and PANSS share the 18 original BPRS items and each has additional unique items (6 BPRS-E and 12 PANSS). Based on the combined data across the BPRS-E and PANSS four separate sets of factor analyses were conducted using sample weighting (weighted and unweighted) and matrix type (similarity coefficients and reproduced correlations). Read More

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BRCC3 promotes activation of the NLRP6 inflammasome following Cerebral Ischemia/Reperfusion (I/R) Injury in Rats.

Neurosci Lett 2021 May 9:135954. Epub 2021 May 9.

Department of Pathology, Chongqing Medical University, Chongqing, CQ, 400016, China. Electronic address:

NOD-like receptor family pyrin domain containing 6 (NLRP6), a novel member of the NLR family, has been confirmed to have an inflammasome-dependent proinflammatory effect in cerebral ischemia/reperfusion injury. NLRP6 assembles a multimeric inflammasome complex comprising the adaptor ASC and the effector pro-caspase-1 to mediate the activation of caspase-1. The molecular mechanism regulating activation of the NLRP6 inflammasome remains unclear. Read More

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The influence of the trunk muscle activation on occlusion pressure and respiratory muscle strength in healthy participants: randomized controlled trial.

Respir Physiol Neurobiol 2021 May 9:103682. Epub 2021 May 9.

Faculty of Health Sciences, Jagiellonian University Medical College, Kraków, Poland. Electronic address:

Objectives: The purpose of the study was to check if the trunk muscle activation done in accordance with rules of the Bobath concept affects the occlusion pressure and respiratory muscle efficacy in forced breathing manoeuvres in healthy participants.

Design: Randomized controlled trial. Between-subjects design. Read More

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Salicylic acid treatment mitigates chilling injury in peach fruit by regulation of sucrose metabolism and soluble sugar content.

Food Chem 2021 Apr 20;358:129867. Epub 2021 Apr 20.

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China. Electronic address:

Peach fruit stored in the cold are susceptible to chilling injury. A pre-storage treatment with the natural hormone salicylic acid can alleviate chilling damage, although the mechanism is unclear. We found that a treatment with 1 μmol L salicylic acid for 15 min prior to storage at 4 °C delayed and reduced fruit internal browning, a symptom of chilling injury. Read More

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Thorny ground, rocky soil: tissue-specific mechanisms of tumor dormancy and relapse.

Semin Cancer Biol 2021 May 9. Epub 2021 May 9.

Public Health Sciences Division/Translational Research Program, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA. Electronic address:

Disseminated tumor cells (DTCs) spread systemically yet distinct patterns of metastasis indicate a range of tissue susceptibility to metastatic colonization. Distinctions between permissive and suppressive tissues are still being elucidated at cellular and molecular levels. Although there is a growing appreciation for the role of the microenvironment in regulating metastatic success, we have a limited understanding of how diverse tissues regulate DTC dormancy, the state of reversible quiescence and subsequent awakening thought to contribute to delayed relapse. Read More

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Role of pyroptosis in diabetic retinopathy and its therapeutic implications.

Eur J Pharmacol 2021 May 9:174166. Epub 2021 May 9.

Molecular Pharmacology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang Province, China. Electronic address:

Pyroptosis has recently been established as a term of programmed-inflammatory cell death. Pyroptosis is mainly divided into two molecular signaling pathways, including caspase-1-dependent canonical and caspase-4/5/11-dependent non-canonical inflammasome pathways. Extensive investigations have reported inflammasome activation facilitates the maturation and secretion of the inflammatory factors interleukin-1β/18 (IL-1β/18), cleavage of gasdermin D (GSDMD), and leading to the stimulation of pyroptosis-mediated cell death. Read More

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A photoswitchable GPCR-based opsin for presynaptic inhibition.

Neuron 2021 May 10. Epub 2021 May 10.

Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA; Department of Neuroscience, Washington University School of Medicine, St. Louis, MO, USA; Center of Excellence in the Neurobiology of Addiction, Pain, and Emotion, Departments of Anesthesiology and Pain Medicine, and Pharmacology, University of Washington, Seattle, WA, USA. Electronic address:

Optical manipulations of genetically defined cell types have generated significant insights into the dynamics of neural circuits. While optogenetic activation has been relatively straightforward, rapid and reversible synaptic inhibition has proven more elusive. Here, we leveraged the natural ability of inhibitory presynaptic GPCRs to suppress synaptic transmission and characterize parapinopsin (PPO) as a GPCR-based opsin for terminal inhibition. Read More

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VEGF counteracts amyloid-β-induced synaptic dysfunction.

Cell Rep 2021 May;35(6):109121

Institut NeuroMyoGène (INMG), Synaptopathies and Autoantibodies, Institut National de la Santé et de la Recherche Médicale (INSERM), U1217, Centre National de la Recherche Scientifique (CNRS) UMR5310, 69000 Lyon, France; Université Claude Bernard Lyon 1, 69000 Lyon, France. Electronic address:

The vascular endothelial growth factor (VEGF) pathway regulates key processes in synapse function, which are disrupted in early stages of Alzheimer's disease (AD) by toxic-soluble amyloid-beta oligomers (Aβo). Here, we show that VEGF accumulates in and around Aβ plaques in postmortem brains of patients with AD and in APP/PS1 mice, an AD mouse model. We uncover specific binding domains involved in direct interaction between Aβo and VEGF and reveal that this interaction jeopardizes VEGFR2 activation in neurons. Read More

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RIPK1 activation mediates neuroinflammation and disease progression in multiple sclerosis.

Cell Rep 2021 May;35(6):109112

Sanofi, Neurological Diseases, 49 New York Ave., Framingham, MA 01701, USA. Electronic address:

Receptor interacting protein kinase 1 (RIPK1) mediates cell death and inflammatory signaling and is increased in multiple sclerosis (MS) brain samples. Here, we investigate the role of glial RIPK1 kinase activity in mediating MS pathogenesis. We demonstrate RIPK1 levels correlate with MS disease progression. Read More

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Persistent NF-κB activation in muscle stem cells induces proliferation-independent telomere shortening.

Cell Rep 2021 May;35(6):109098

Department of Orthopaedic Surgery, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Cell and Developmental Biology, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA 19104, USA; Institute of Regenerative Medicine, Musculoskeletal Regeneration Program, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address:

During the repeated cycles of damage and repair in many muscle disorders, including Duchenne muscular dystrophy (DMD), the muscle stem cell (MuSC) pool becomes less efficient at responding to and repairing damage. The underlying mechanism of such stem cell dysfunction is not fully known. Here, we demonstrate that the distinct early telomere shortening of diseased MuSCs in both mice and young DMD patients is associated with aberrant NF-κB activation. Read More

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Social isolation impairs the prefrontal-nucleus accumbens circuit subserving social recognition in mice.

Cell Rep 2021 May;35(6):109104

Department of Physiology, Seoul National University College of Medicine, Seoul 03080, Korea; Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Korea; Neuroscience Research Institute, Seoul National University College of Medicine, Seoul 03080, Korea. Electronic address:

Although medial prefrontal cortex (mPFC) is known to play important roles in social behaviors, how early social experiences affect the mPFC and its subcortical circuit remains unclear. We report that mice singly housed (SH) for 8 weeks after weaning show a social recognition deficit, even after 4 weeks of resocialization. In SH mice, prefrontal infralimbic (IL) neurons projecting to the shell region of nucleus accumbens (NAcSh) show decreased excitability compared with group-housed (GH) mice. Read More

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Chromatin accessibility governs the differential response of cancer and T cells to arginine starvation.

Cell Rep 2021 May;35(6):109101

MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, UK.

Depleting the microenvironment of important nutrients such as arginine is a key strategy for immune evasion by cancer cells. Many tumors overexpress arginase, but it is unclear how these cancers, but not T cells, tolerate arginine depletion. In this study, we show that tumor cells synthesize arginine from citrulline by upregulating argininosuccinate synthetase 1 (ASS1). Read More

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Role of nuclear localization in the regulation and function of T-bet and Eomes in exhausted CD8 T cells.

Cell Rep 2021 May;35(6):109120

Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Parker Institute for Cancer Immunotherapy, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address:

The transcription factors T-bet and Eomesodermin (Eomes) regulate CD8 T cell exhaustion through undefined mechanisms. Here, we show that the subcellular localization of T-bet and Eomes dictate their regulatory activity in exhausted T cells (Ts). Ts had a higher ratio of nuclear Eomes:T-bet than memory T cells (Ts) during chronic lymphocytic choriomeningitis virus (LCMV) infection in preclinical cancer models and in human tumors. Read More

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α-Synuclein mutation impairs processing of endomembrane compartments and promotes exocytosis and seeding of α-synuclein pathology.

Cell Rep 2021 May;35(6):109099

Department of Molecular and Cellular Biology, The University of Guelph, Guelph, ON N1G 2W1, Canada; Neurodegenerative Disease Center, Scintillon Institute, 6868 Nancy Ridge Drive, San Diego, CA 92121, USA. Electronic address:

Neuronal loss in Parkinson's disease (PD) is associated with impaired proteostasis and accumulation of α-syn microaggregates in dopaminergic neurons. These microaggregates promote seeding of α-synuclein (α-syn) pathology between synaptically linked neurons. However, the mechanism by which seeding is initiated is not clear. Read More

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Glycolytic ATP fuels phosphoinositide 3-kinase signaling to support effector T helper 17 cell responses.

Immunity 2021 May;54(5):976-987.e7

Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Immunology and Microbial Pathogenesis Graduate Program, Weill Cornell Graduate School of Medical Sciences, Cornell University, New York, NY 10065, USA; Louis V. Gerstner Jr. Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA. Electronic address:

Aerobic glycolysis-the Warburg effect-converts glucose to lactate via the enzyme lactate dehydrogenase A (LDHA) and is a metabolic feature of effector T cells. Cells generate ATP through various mechanisms and Warburg metabolism is comparatively an energy-inefficient glucose catabolism pathway. Here, we examined the effect of ATP generated via aerobic glycolysis in antigen-driven T cell responses. Read More

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MDSC: Markers, development, states, and unaddressed complexity.

Immunity 2021 May;54(5):875-884

The Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA; The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA; Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA; Human Immune Monitoring Center, Icahn School of Medicine at Mount Sinai, New York, NY, USA. Electronic address:

Myeloid-derived suppressor cells (MDSCs) are one of the most discussed biological entities in immunology. While the context and classification of this group of cells has evolved, MDSCs most commonly describe cells arising during chronic inflammation, especially late-stage cancers, and are defined by their T cell immunosuppressive functions. This MDSC concept has helped explain myeloid phenomena associated with disease outcome, but currently lacks clear definitions and a unifying framework across pathologies. Read More

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Shining light on the neuro-immune axis in the gut.

Immunity 2021 May;54(5):850-852

Center of Intestinal Neuro-immune Interaction, Translational Research Center for GI Disorders (TARGID), Department of Chronic Diseases, Metabolism and Ageing, KU Leuven - University of Leuven, Leuven, Belgium. Electronic address:

In this issue of Immunity, Schiller et al. report that local sympathetic nerve activation decreases endothelial expression of the adhesion molecule MAdCAM-1, reducing immune cell infiltration and colitis-induced inflammation. These findings suggest that local sympathetic stimulation provides a key gateway for regulating organ homeostasis. Read More

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Time will tell: The temporal code of immune threats.

Immunity 2021 May;54(5):845-847

Department of Immunology and Center for Systems Immunology, University of Pittsburgh, Pittsburgh, PA, USA. Electronic address:

Activation of NF-κB is a common downstream consequence of inflammatory stimulation, yet it achieves stimulus-specific transcriptional responses. In this issue of Immunity, Adelaja et al. use single-cell imaging and computational approaches to understand temporal features of NF-κB dynamics that transmit information about immune threats. Read More

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The inhibitory receptor TIM-3 limits activation of the cGAS-STING pathway in intra-tumoral dendritic cells by suppressing extracellular DNA uptake.

Immunity 2021 May 5. Epub 2021 May 5.

Department of Immunology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA; Department of Breast Oncology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA. Electronic address:

Blockade of the inhibitory receptor TIM-3 shows efficacy in cancer immunotherapy clinical trials. TIM-3 inhibits production of the chemokine CXCL9 by XCR1 classical dendritic cells (cDC1), thereby limiting antitumor immunity in mammary carcinomas. We found that increased CXCL9 expression by splenic cDC1s upon TIM-3 blockade required type I interferons and extracellular DNA. Read More

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Phragmunis A suppresses glioblastoma through the regulation of MCL1-FBXW7 by blocking ELK1-SRF complex-dependent transcription.

Neurochem Int 2021 May 9:105051. Epub 2021 May 9.

Department of Neurosurgery, Shenzhen Key Laboratory of Neurosurgery, the First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 3002# Sungang Road, Futian District, Shenzhen 518035, China. Electronic address:

Glioblastoma (GBM) is a highly aggressive brain tumour. During screening work, we found a new compound named phragmunis A (PGA), which is derived from the fruitbody of Trogia venenata, exhibits a potential cytotoxic effect on patient-derived recurrent GBM cells and temozolomide (TMZ)-resistant cell lines. The present study was designed to investigate the potential molecular mechanism of the anti-glioma effects of PGA in vitro and in vivo. Read More

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FoxO1 in Micropterus salmoides: Molecular characterization and its roles in glucose metabolism by glucose or insulin-glucose loading.

Gen Comp Endocrinol 2021 May 9:113811. Epub 2021 May 9.

National Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. Electronic address:

Forkhead box O1 (FoxO1), a nuclear transcription factor, plays an important role in insulin-mediated glucose metabolism. In this study, FoxO1 gene from largemouth bass (Micropterus salmoides) was cloned and characterized, and its effects on hepatic glucose metabolism regulated by insulin-AKT pathway were investigated in response to glucose or insulin-glucose injection. The full-length cDNA of FoxO1 consisted of 2541 bp and encoded 680 amino acids. Read More

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Cultured human uveal melanocytes express/secrete CXCL1 and CXCL2 constitutively and increased by lipopolysaccharide via activation of Toll-like receptor 4.

Curr Eye Res 2021 May 12. Epub 2021 May 12.

Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.

Purpose: Lipopolysaccharide (LPS) can activate Toll-like receptor 4 (TLR4) and increase the expression of CXCL1 and CXCL2, the potent neutrophils chemoattractants, in various cell types. These effects have not been previously reported in the uveal melanocytes. This study was designed to investigate the effects of LPS on the activation of TLR4 and expression of CXCL1/CXCL2 in cultured human uveal melanocytes and the relevant signal pathways. Read More

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Hydrogen Bonding Strength Determines Water Diffusivity in Polymer Ionogels.

J Phys Chem B 2021 May 12. Epub 2021 May 12.

Department of Chemical Engineering, University of California Santa Barbara, Santa Barbara 93106-9010, United States.

Polymeric ionogels, cross-linked gels swollen by ionic liquids (ILs), are useful vehicles for the release and storage of molecular solutes in separation, delivery, and other applications. Although rapid solute diffusion is often critical for performance, it remains challenging to predict diffusivities across multidimensional composition spaces. Recently, we showed that water (a neutral solute) diffuses through alkyl-methylimidazolium halide ILs by hopping between hydrogen bonding sites on relatively immobile cations. Read More

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Cationic Heterobimetallic Mg(Zn)/Al(Ga) Combinations for Cooperative C-F Bond Cleavage.

Angew Chem Int Ed Engl 2021 May 12. Epub 2021 May 12.

FAU: Friedrich-Alexander-Universitat Erlangen-Nurnberg, chemistry, GERMANY.

Low-valent ( Me BDI)Al and ( Me BDI)Ga act as Lewis bases for complexation of highly Lewis acidic cations in [( tBu BDI)M + ·C 6 H 6 ][(B(C 6 F 5 ) 4 -] (M = Mg or Zn, Me BDI = HC[C(Me)N-DIPP], tBu BDI = HC[C( t Bu)N-DIPP], DIPP = 2,6-diisopropylphenyl) yielding heterobimetallic complexes containing the cations [( tBu BDI)Mg-Al( Me BDI) + ], [( tBu BDI)Mg-Ga( Me BDI) + ] and [( tBu BDI)Zn-Ga( Me BDI) + ]. These cations do not show interaction with B(C 6 F 5 ) 4 - and feature long Mg-Al(or Ga) distances or a short Zn-Ga bond. The complex with the [( tBu BDI)Zn-Al( Me BDI) + ] cation could not be obtained but should, according to DFT calculations, be stable. Read More

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Copper-Catalyzed Dehydrogenative Amidation of Light Alkanes.

Angew Chem Int Ed Engl 2021 May 12. Epub 2021 May 12.

Universidad de Huelva, Quimica y Ciencia de Materiales, Campus de El Carmen, 21007, Huelva, SPAIN.

The functionalization of C-H bonds in light alkanes, particularly to form C-N bonds, remains a challenge. We report the dehydrogenative coupling of amides with these C1-C4 hydrocarbons to form the N -alkyl amide products with t BuOO t Bu as the oxidant, and a copper catalyst ligated by a phenanthroline-type ligand. The reactions occurred in good yields in benzene or supercritical carbon dioxide as solvents. Read More

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