12,675 results match your criteria pili

Characterization of Pasteurella multocida isolated from ducks in China from 2017 to 2019.

Microb Pathog 2021 Sep 14:105196. Epub 2021 Sep 14.

College of Animal Science, South China Agricultural University, Guangzhou, China. Electronic address:

Pasteurella multocida, an important gram-negative pathogen that mainly inhibits the upper respiratory tracts of domestic and wild animals such as chicken, duck, cattle and pig, which can cause cholera fowl, haemorrhagic septicaemia and infectious pneumonia. Currently, the prevalence and infection of Pasteurella multocida is still one of the most serious threats to the poultry industry in China, but studies on its characteristics are still insufficient. Here, this study was conducted to isolate and identify Pasteurella multocida in infected ducks and determined the leading serotypes and epidemiology of the diseases this pathogen causes. Read More

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September 2021

Evolution of over a 23-year period in France, 1996 to 2018.

Euro Surveill 2021 09;26(37)

Institut Pasteur, Biodiversity and Epidemiology of Bacterial Pathogens, Paris, France.

Background is the main agent of whooping cough. Vaccination with acellular pertussis vaccines has been largely implemented in high-income countries. These vaccines contain 1 to 5 antigens: pertussis toxin (PT), filamentous haemagglutinin (FHA), pertactin (PRN) and/or fimbrial proteins (FIM2 and FIM3). Read More

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September 2021

Quorum sensing regulates transcription of the pilin gene mshA1 of MSHA pilus in Vibrio parahaemolyticus.

Gene 2021 Sep 13:145961. Epub 2021 Sep 13.

Wuxi School of Medicine, Jiangnan University, Wuxi 214122, Jiangsu, China. Electronic address:

Vibrio parahaemolyticus produces two types of IV pili: mannose-sensitive haemagglutinin type IV pili (MSHA) and chitin-regulated pili (ChiRP). Both of them are required for biofilm formation and the pathogen persistence in hosts. However, there are few reports on the regulation of their expression. Read More

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September 2021

Piloleiomyosarcoma in cats: Histological and immunohistochemical features.

Vet Pathol 2021 Sep 16:3009858211042582. Epub 2021 Sep 16.

University of Las Palmas de Gran Canaria, Arucas, Gran Canaria, Spain.

This study describes the histomorphology and immunohistochemical profile of 9 cases of feline piloleiomyosarcoma. Cats ranged in age from 7 to 16 years (mean 10), and tumors were 7 to 24 mm in diameter (mean 15). Tumors were composed of fusiform cells that were haphazardly arranged or in variably sized interwoven bundles. Read More

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September 2021

Computational and biochemical analysis of type IV pilus dynamics and stability.

Structure 2021 Sep 11. Epub 2021 Sep 11.

Structural Bioinformatics Unit, Department of Structural Biology and Chemistry, Institut Pasteur, CNRS UMR3528, Paris, France. Electronic address:

Type IV pili (T4P) are distinctive dynamic filaments at the surface of many bacteria that can rapidly extend and retract and withstand strong forces. T4P are important virulence factors in many human pathogens, including Enterohemorrhagic Escherichia coli (EHEC). The structure of the EHEC T4P has been determined by integrating nuclear magnetic resonance (NMR) and cryo-electron microscopy data. Read More

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September 2021

COVID-19 vaccine hesitancy in patients on dialysis in Italy and France.

Kidney Int Rep 2021 Sep 8. Epub 2021 Sep 8.

Néphrologie et Dialyse, Centre Hospitalier Le Mans, 194 Avenue Rubillard, 72037, Le Mans, France.

Introduction: Patients on dialysis (HDP) are a category at high risk from COVID-19 and thus a high-priority group for vaccination. COVID-19 vaccine hesitancy has been a concern since the availability of the first vaccine. The objective of this study was to determine hesitancy rates and factors associated with hesitancy towards COVID-19 vaccination in HDP. Read More

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September 2021

Importance of twitching and surface-associated motility in the virulence of .

Virulence 2021 12;12(1):2201-2213

Departament De Genètica I Microbiologia, Facultat De Biociènces, Universitat Autònoma De Barcelona, Barcelona, Spain.

is a pathogen of increasing clinical importance worldwide, especially given its ability to readily acquire resistance determinants. Motile strains of this bacterium can move by either or both of two types of motility: (i) twitching, driven by type IV pili, and (ii) surface-associated motility, an appendage-independent form of movement. strain MAR002 possesses both twitching and surface-associated motility. Read More

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December 2021

Rhizobial Chemotaxis and Motility Systems at Work in the Soil.

Front Plant Sci 2021 27;12:725338. Epub 2021 Aug 27.

Department of Plant Sciences, University of Oxford, Oxford, United Kingdom.

Bacteria navigate their way often as individual cells through their chemical and biological environment in aqueous medium or across solid surfaces. They swim when starved or in response to physical and chemical stimuli. Flagella-driven chemotaxis in bacteria has emerged as a paradigm for both signal transduction and cellular decision-making. Read More

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Prospects for the Mechanism of Swimming.

Front Microbiol 2021 27;12:706426. Epub 2021 Aug 27.

Department of Biology, Graduate School of Science, Osaka City University, Osaka, Japan.

are helical bacteria that lack a peptidoglycan layer. They are widespread globally as parasites of arthropods and plants. Their infectious processes and survival are most likely supported by their unique swimming system, which is unrelated to well-known bacterial motility systems such as flagella and pili. Read More

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A Tad-like apparatus is required for contact-dependent prey killing in predatory social bacteria.

Elife 2021 Sep 10;10. Epub 2021 Sep 10.

CNRS-Aix Marseille University, Marseille, France.

, a soil bacterium, predates collectively using motility to invade prey colonies. Prey lysis is mostly thought to rely on secreted factors, cocktails of antibiotics and enzymes, and direct contac with cells. In this study, we show that on surfaces the coupling of A-motility and contact-dependent killing is the central predatory mechanism driving effective prey colony invasion and consumption. Read More

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September 2021

Pili Torti: A Feature of Numerous Congenital and Acquired Conditions.

J Clin Med 2021 Aug 30;10(17). Epub 2021 Aug 30.

Department of Dermatology, Medical University of Warsaw, Koszykowa 82A, 02-008 Warsaw, Poland.

Pili torti is a rare condition characterized by the presence of the hair shaft, which is flattened at irregular intervals and twisted 180° along its long axis. It is a form of hair shaft disorder with increased fragility. The condition is classified into inherited and acquired. Read More

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Optorheological Characteristics of Photosynthetic Bacterium Suspension.

Langmuir 2021 Sep 7. Epub 2021 Sep 7.

Department of Fiber Convergence Materials Engineering, Dankook University, Gyeonggi-do 16890, Korea.

Understanding the rheological behavior of materials is of great importance in science. Here, we report a microscopic foundation for optorheology by manipulating the rheological feature through light. A new phenomenon is observed in the photosynthetic bacterial suspension, that the fluid viscosity changes by light-induced electrons. Read More

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September 2021

Whole Genome Sequencing of Pediatric Strains Reveals Important Insights Into Their Virulence-Associated Traits.

Front Microbiol 2021 13;12:711577. Epub 2021 Aug 13.

Unidad de Investigación Médica en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico.

is recognized as a common cause of nosocomial infections and outbreaks causing pneumonia, septicemia, and urinary tract infections. This opportunistic bacterium shows an increasing acquisition of antibiotic-resistance genes, which complicates treatment of infections. Hence, fast reliable strain typing methods are paramount for the study of this opportunistic pathogen's multi-drug resistance genetic profiles. Read More

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Corksorb Enhances Alkane Degradation by Hydrocarbonoclastic Bacteria.

Front Microbiol 2021 19;12:618270. Epub 2021 Aug 19.

CEB - Centre of Biological Engineering, University of Minho, Braga, Portugal.

Biosorbent materials are effective in the removal of spilled oil from water, but their effect on hydrocarbonoclastic bacteria is not known. Here, we show that corksorb, a cork-based biosorbent, enhances growth and alkane degradation by B4 (Ro) and SK2 (Ab). Ro and Ab degraded 96 ± 1% and 72 ± 2%, respectively, of a mixture of -alkanes (2 g L) in the presence of corksorb. Read More

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Observation of New Resonances Decaying to J/ψK^{+} and J/ψϕ.

Phys Rev Lett 2021 Aug;127(8):082001

Laboratoire Leprince-Ringuet, CNRS/IN2P3, Ecole Polytechnique, Institut Polytechnique de Paris, Palaiseau, France.

The first observation of exotic states with a new quark content cc[over ¯]us[over ¯] decaying to the J/ψK^{+} final state is reported with high significance from an amplitude analysis of the B^{+}→J/ψϕK^{+} decay. The analysis is carried out using proton-proton collision data corresponding to a total integrated luminosity of 9  fb^{-1} collected by the LHCb experiment at center-of-mass energies of 7, 8, and 13 TeV. The most significant state, Z_{cs}(4000)^{+}, has a mass of 4003±6_{-14}^{+4}  MeV, a width of 131±15±26  MeV, and spin parity J^{P}=1^{+}, where the quoted uncertainties are statistical and systematic, respectively. Read More

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Model systems for optical trapping: the physical basis and biological applications.

Biophys Rev 2021 Aug 27;13(4):515-529. Epub 2021 Jul 27.

Institute of Physiology of Коmi Science Centre of the Ural Branch of the Russian Academy of Sciences, FRC Komi SC UB RAS, Komi Republic, 167982 Syktyvkar, Russian Federation.

The micromechanical methods, among which optical trapping and atomic force microscopy have a special place, are widespread currently in biology to study molecular interactions between different biological objects. Optical trapping is reported to be quite applicable to study the mechanical properties of surface structures onto bacterial (pili and flagella) and eukaryotic (filopodia) cells. The review briefly summarizes the physical basis of optical trapping, as well as the principles of calculating the van der Waals, electrostatic, and donor-acceptor forces when two microparticles or a microparticle and a flat surface are used. Read More

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Structure of Geobacter pili reveals secretory rather than nanowire behaviour.

Nature 2021 09 1;597(7876):430-434. Epub 2021 Sep 1.

Microbial Sciences Institute Yale University, West Haven, CT, USA.

Extracellular electron transfer by Geobacter species through surface appendages known as microbial nanowires is important in a range of globally important environmental phenomena, as well as for applications in bio-remediation, bioenergy, biofuels and bioelectronics. Since 2005, these nanowires have been thought to be type 4 pili composed solely of the PilA-N protein. However, previous structural analyses have demonstrated that, during extracellular electron transfer, cells do not produce pili but rather nanowires made up of the cytochromes OmcS and OmcZ. Read More

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September 2021

Processive dynamics of the usher assembly platform during uropathogenic Escherichia coli P pilus biogenesis.

Nat Commun 2021 09 1;12(1):5207. Epub 2021 Sep 1.

Department of Microbiology and Immunology, Stony Brook University, Stony Brook, New York, NY, USA.

Uropathogenic Escherichia coli assemble surface structures termed pili or fimbriae to initiate infection of the urinary tract. P pili facilitate bacterial colonization of the kidney and pyelonephritis. P pili are assembled through the conserved chaperone-usher pathway. Read More

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September 2021

Cell wall deficiency as an escape mechanism from phage infection.

Open Biol 2021 Sep 1;11(9):210199. Epub 2021 Sep 1.

Molecular Biotechnology, Institute of Biology, Leiden University, PO Box 9505, 2300 RA Leiden, The Netherlands.

The cell wall plays a central role in protecting bacteria from some environmental stresses, but not against all. In fact, in some cases, an elaborate cell envelope may even render the cell more vulnerable. For example, it contains molecules or complexes that bacteriophages recognize as the first step of host invasion, such as proteins and sugars, or cell appendages such as pili or flagella. Read More

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September 2021

Direct Observation of Electrically Conductive Pili Emanating from .

mBio 2021 Aug 31;12(4):e0220921. Epub 2021 Aug 31.

Department of Microbiology, University of Massachusetts-Amherst, Amherst, Massachusetts, USA.

Geobacter sulfurreducens is a model microbe for elucidating the mechanisms for extracellular electron transfer in several biogeochemical cycles, bioelectrochemical applications, and microbial metal corrosion. Multiple lines of evidence previously suggested that electrically conductive pili (e-pili) are an essential conduit for long-range extracellular electron transport in G. sulfurreducens. Read More

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Tight Adherence (Tad) Pilus Genes Indicate Putative Niche Differentiation in Phytoplankton Bloom Associated .

Front Microbiol 2021 10;12:718297. Epub 2021 Aug 10.

Marine Microbial Ecology Laboratory, Biology Program, New York University Abu Dhabi, Abu Dhabi, United Arab Emirates.

The multiple interactions of phytoplankton and bacterioplankton are central for our understanding of aquatic environments. A prominent example of those is the consistent association of diatoms with of the order . These photoheterotrophic bacteria have traditionally been described as generalists that scavenge dissolved organic matter. Read More

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Role of the YehD fimbriae in the virulence-associated properties of enteroaggregative Escherichia coli.

Environ Microbiol 2021 Aug 25. Epub 2021 Aug 25.

Department of Pediatrics, University of Virginia School of Medicine, Charlottesville, VA, USA.

The interaction of enteroaggregative Escherichia coli (EAEC) strains with the colonic gut mucosa is characterized by the ability of the bacteria to form robust biofilms, to bind mucin, and induce a local inflammatory response. These events are mediated by a repertoire of five different aggregative adherence fimbriae variants (AAF/I-V) typically encoded on virulence plasmids. In this study, we report the production in EAEC strains of a new YehD fimbriae (YDF), which is encoded by the chromosomal gene cluster yehABCD, also present in most E. Read More

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Rapid removal of chloramphenicol via the synergy of Geobacter and metal oxide nanoparticles.

Chemosphere 2021 Aug 20;286(Pt 3):131943. Epub 2021 Aug 20.

Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, PR China. Electronic address:

The wide use of chloramphenicol and its residues in the environments are an increasing threat to human beings. Electroactive microorganisms were proven with the ability of biodegradation of chloramphenicol, but the removal rate and efficiency need to be improved. In this study, a model electricigens, Geobacter metallireducens, was supplied with and FeO and MnO nanoparticles. Read More

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Blockage of bacterial FimH prevents mucosal inflammation associated with Crohn's disease.

Microbiome 2021 08 23;9(1):176. Epub 2021 Aug 23.

Enterome, 94-96 Avenue Ledru-Rollin, 75011, Paris, France.

Background: An Escherichia coli (E. coli) pathotype with invasive properties, first reported by Darfeuille-Michaud and termed adherent-invasive E. coli (AIEC), was shown to be prevalent in up to half the individuals with Crohn's Disease (CD), suggesting that these bacteria could be involved in the pathophysiology of CD. Read More

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H,  N and C resonance assignments of the C-terminal domain of PulL, a component of the Klebsiella oxytoca type II secretion system.

Biomol NMR Assign 2021 Aug 19. Epub 2021 Aug 19.

Structural Bioinformatics Unit, Department of Structural Biology and Chemistry, CNRS UMR3528, Institut Pasteur, 28 rue du Dr Roux, 75724, Paris, France.

Type II secretion systems (T2SS) allow Gram-negative bacteria to transport toxins and enzymes from the periplasm to the external milieu, and are thus important for the pathogenicity of bacteria. To drive secretion, T2SS assemble filaments called pseudopili closely related to bacterial type IV pili. These filaments are non-covalent polymers of proteins that are assembled by an inner membrane complex called the assembly platform connected to a cytoplasmic ATPase motor. Read More

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Molecular epidemiology of Bordetella pertussis and analysis of vaccine antigen genes from clinical isolates from Shenzhen, China.

Ann Clin Microbiol Antimicrob 2021 Aug 18;20(1):53. Epub 2021 Aug 18.

Shenzhen Center for Disease Control and Prevention, 8 Longyuan Road, Nanshan District, Shenzhen, China.

Background: Although pertussis cases globally have been controlled through the Expanded Programme on Immunization (EPI), the incidence of pertussis has increased significantly in recent years, with a "resurgence" of pertussis occurring in developed countries with high immunization coverage. Attracted by its fast-developing economy, the population of Shenzhen has reached 14 million and has become one of the top five largest cities by population size in China. The incidence of pertussis here was about 2. Read More

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The PilB-PilZ-FimX regulatory complex of the Type IV pilus from Xanthomonas citri.

PLoS Pathog 2021 Aug 16;17(8):e1009808. Epub 2021 Aug 16.

Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.

Type IV pili (T4P) are thin and flexible filaments found on the surface of a wide range of Gram-negative bacteria that undergo cycles of extension and retraction and participate in a variety of important functions related to lifestyle, defense and pathogenesis. During pilus extensions, the PilB ATPase energizes the polymerization of pilin monomers from the inner membrane. In Xanthomonas citri, two cytosolic proteins, PilZ and the c-di-GMP receptor FimX, are involved in the regulation of T4P biogenesis through interactions with PilB. Read More

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Plasmid-encoded H-NS controls extracellular matrix composition in a modern urinary isolate.

J Bacteriol 2021 Aug 16:JB0027721. Epub 2021 Aug 16.

Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO, 63110, USA.

is emerging as a multidrug-resistant (MDR) nosocomial pathogen of increasing threat to human health worldwide. The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCP). LCP encode several antibiotic resistance genes and repress the type VI secretion system (T6SS) to enable their dissemination, employing two TetR transcriptional regulators. Read More

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Spatial transcriptomics of planktonic and sessile bacterial populations at single-cell resolution.

Science 2021 08;373(6556)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.

Capturing the heterogeneous phenotypes of microbial populations at relevant spatiotemporal scales is highly challenging. Here, we present par-seqFISH (parallel sequential fluorescence in situ hybridization), a transcriptome-imaging approach that records gene expression and spatial context within microscale assemblies at a single-cell and molecule resolution. We applied this approach to the opportunistic pathogen , analyzing about 600,000 individuals across dozens of conditions in planktonic and biofilm cultures. Read More

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