877 results match your criteria phospholipase carried

Safety Evaluation of Yeasts With Probiotic Potential.

Front Nutr 2021 21;8:659328. Epub 2021 May 21.

Analytical Chemistry and Food Technology Department and Instituto Regional de Investigación Científica Aplicada (IRICA), Faculty of Chemical Sciences and Technologies, Castilla-La Mancha University, Ciudad Real, Spain.

This work has evaluated the safety aspects of 20 yeast strains, isolated from food environments, selected in previous works due to their probiotic potential. Among the different strains, there are and non- yeasts. Before safety evaluation, differentiation of strains was done by PCR amplification of inter-δ region with pairs of primers δ2-12 and δ12-21, which showed that they were all different from each other and also had different profiles to (the only commercial probiotic yeast). Read More

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Lipoprotein-Associated Phospholipase A2 Is a Risk Factor for Patients With Parkinson's Disease.

Front Neurosci 2021 20;15:633022. Epub 2021 Apr 20.

Department of Clinical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Objective: To explore the association between lipoprotein-related phospholipase A2 (Lp-PLA2) and the risk of Parkinson's disease (PD).

Methods: A case-control study involving 58 hospitalized PD patients and 60 healthy controls was carried out. Serum Lp-PLA2 level was detected. Read More

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Single-center thorough evaluation and targeted treatment of globozoospermic men.

J Assist Reprod Genet 2021 Apr 20. Epub 2021 Apr 20.

The Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine, Weill Cornell Medicine, 1305 York Avenue, Y720, New York, NY, 10021, USA.

Purpose: To characterize, by specific biomarkers and nucleic acid sequencing, the structural and genomic sperm characteristics of partial (PG) and complete globozoospermic (CG) men in order to identify the best reproductive treatment.

Methods: We assessed spermatozoa from 14 consenting men ultrastructurally, as well as for histone content, sperm chromatin integrity, and sperm aneuploidy. Additional genomic, transcriptomic, and proteomic evaluations were carried out to further characterize the CG cohort. Read More

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Antibacterial and Antifungal Activities and Toxicity of the Essential Oil from Callistemon viminalis Complexed with β-Cyclodextrin.

Curr Microbiol 2021 Jun 10;78(6):2251-2258. Epub 2021 Apr 10.

Departamento de Química, Universidade Federal de Lavras, C.P. 3037, Lavras, MG, CEP 37.200-900, Brazil.

The essential oil from Callistemon viminalis (EOC) is rich in monoterpenes, with a variety of biological properties: antibacterial, antifungal, insecticide, and antioxidant. Inclusion complexes (ICs) with cyclodextrins (CDs) is an alternative to prevent toxicity, improve the activity, and reduce the concentration to be used. Thus, the objective of this work was to prepare an IC (EOC/β-CD) and evaluate the antibacterial, antifungal and phospholipase activities, as well as the toxicity. Read More

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Fetal High-Density Lipoproteins: Current Knowledge on Particle Metabolism, Composition and Function in Health and Disease.

Biomedicines 2021 Mar 30;9(4). Epub 2021 Mar 30.

Division of Pharmacology, Otto Loewi Research Center, Medical University of Graz, Universitätsplatz 4, 8010 Graz, Austria.

Cholesterol and other lipids carried by lipoproteins play an indispensable role in fetal development. Recent evidence suggests that maternally derived high-density lipoprotein (HDL) differs from fetal HDL with respect to its proteome, size, and function. Compared to the HDL of adults, fetal HDL is the major carrier of cholesterol and has a unique composition that implies other physiological functions. Read More

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PNPLA3 Single Nucleotide Polymorphism Prevalence and Association with Liver Disease in a Diverse Cohort of Persons Living with HIV.

Biology (Basel) 2021 Mar 21;10(3). Epub 2021 Mar 21.

Robert Stempel School of Public Health, Florida International University, Miami, FL 33199, USA.

In persons living with HIV (PLWH), there are multiple sources of liver injury. Gene polymorphisms of PNPLA3 (patatin-like phospholipase domain-containing protein 3) have been identified as an important cofactor for increased disease severity in both alcoholic and non-alcoholic steatohepatitis (NASH). We utilized a well-characterized cohort of ethnically and racially diverse patients with HIV to define the prevalence of PNPLA3 SNPs (single nucleotide polymorphism) (rs738409), and to determine the relationship to hepatic steatosis and liver fibrosis. Read More

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Differential Effects of Serum Lipoprotein-Associated Phospholipase A2 on Periventricular and Deep Subcortical White Matter Hyperintensity in Brain.

Front Neurol 2021 8;12:605372. Epub 2021 Mar 8.

Department of Neurology, The Affiliated Jiangning Hospital With Nanjing Medical University, Nanjing, China.

Serum level of lipoprotein-associated phospholipase A2 (Lp-PLA2) was associated with white matter hyperintensity (WMH). There were differences in the anatomical structure and pathophysiological mechanism between periventricular WMH (PVWMH) and deep subcortical WMH (DSWMH). In this study, we aimed to investigate the effects of serum Lp-PLA2 on the PVWMH and DSWMH. Read More

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[Correlation between PNPLA3 rs738409 and TM6SF2 rs58542926 gene polymorphism and primary liver cancer in the Han Population of China's Northeast region].

H Bing W Wang Y L Li

Zhonghua Gan Zang Bing Za Zhi 2021 Feb;29(2):156-162

Department of Gastroenterology, First Affiliated Hospital of China Medical University, Shenyang 110001, China.

To investigate the correlation between patatin-like phospholipase domain-containing 3 (PNPLA3) rs738409 and transmembrane 6 superfamily member 2 (TM6SF2) rs58542926 gene polymorphisms and the incidence of primary liver cancer in the Han population of China's Northeast region. A case-control study was used to enroll 521 patients with primary liver cancer as the case group and 164 healthy people as the control group. The case group was divided into groups with and without liver cirrhosis according to etiology. Read More

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February 2021

Phospholipid signaling pathway in Capsicum chinense suspension cells as a key response to consortium infection.

BMC Plant Biol 2021 Jan 25;21(1):62. Epub 2021 Jan 25.

Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico.

Background: Mexico is considered the diversification center for chili species, but these crops are susceptible to infection by pathogens such as Colletotrichum spp., which causes anthracnose disease and postharvest decay in general. Studies have been carried out with isolated strains of Colletotrichum in Capsicum plants; however, under growing conditions, microorganisms generally interact with others, resulting in an increase or decrease of their ability to infect the roots of C. Read More

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January 2021

Two-stage Enzymatic Hydrolysis of Soybean Concentrated Phospholipid to Prepare Glycerylphosphorylcholine: Optimized by Response Surface Methodology.

J Oleo Sci 2021 Feb 15;70(2):237-245. Epub 2021 Jan 15.

College of Food Science and Engineering, Henan University of Technology.

A two-stage enzymatic hydrolysis method, in which phospholipase A (PLA) was added after phospholipase A (PLA) was added for a certain time, was successfully carried out to prepare glycerylphosphorylcholine (GPC) from soybean concentrated phospholipid. Effects of reaction variables on hydrolysis reaction were optimized using response surface methodology, and the optimal conditions were as follows: PLA load of 1.25%, PLA load of 0. Read More

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February 2021

Human Tyrosyl-DNA Phosphodiesterase 1 Possesses Transphosphooligonucleotidation Activity With Primary Alcohols.

Front Cell Dev Biol 2020 23;8:604732. Epub 2020 Dec 23.

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.

Human tyrosyl-DNA phosphodiesterase 1 (TDP1) belongs to the phospholipase D superfamily, whose members contain paired catalytic histidine and lysine residues within two conserved motifs and hydrolyze phosphodiester bonds. TDP1 is a DNA repair enzyme that processes 3' DNA end blocking lesions and a wide range of synthetic DNA adducts as a substrate. TDP1 hydrolyzes DNA-adducts via two coordinated S2 nucleophilic attacks mediated by the action of two histidine residues and leads to the formation of the covalent intermediate. Read More

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December 2020

Efficient secretion expression of phospholipase D in Bacillus subtilis and its application in synthesis of phosphatidylserine by enzyme immobilization.

Int J Biol Macromol 2021 Feb 15;169:282-289. Epub 2020 Dec 15.

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, State Key Laboratory of Food Nutrition and Safety, The College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China. Electronic address:

Transphosphatidylation catalyzed by phospholipase D has gained increasing attention for producing phosphatidylserine (PS), which can be used in functional food and medicine. In this study, we investigated the effects of six signal peptides on the secretion of PLD (PLD) from Streptomyces antibioticus TCCC 21059 in the food-grade GRAS bacterium Bacillus subtilis. It indicated that the optimal signal peptide DacB with an Ala-X-Ala sequence motif at the C-terminus showed the highest secretory expression ability, resulting in increased production of 2. Read More

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February 2021

In vitro anti-venom potential of various solvent based leaf extracts of Andrographis serpyllifolia (Rottler ex Vahl) Wight against Naja naja and Daboia russelli.

J Ethnopharmacol 2021 Apr 11;269:113687. Epub 2020 Dec 11.

Department of Botany, Bharathiar University, Coimbatore, 46, TN, India. Electronic address:

Ethnopharmacological Relevance: Snake bite is a major occupational hazard in tropical and subtropical countries including India as per the World Health Organization. Naja naja (Indian cobra) and Daboia russelli (Russell's viper) are the two poisonous snakes commonly associated with human mortality in India. Andrographis serpyllifolia (Rottler ex Vahl) Wight has been documented in ethnobotanical records as a plant possessing potent anti-snake venom activity. Read More

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An evidence based efficacy and safety assessment of the ethnobiologicals against poisonous and non-poisonous bites used by the tribals of three westernmost districts of West Bengal, India: Anti-phospholipase A2 and genotoxic effects.

PLoS One 2020 30;15(11):e0242944. Epub 2020 Nov 30.

Department of Medical Biochemistry, College of Medicine & Health Sciences, University of Gondar, Gondar, Ethiopia.

Introduction: To explore the ethnobiological wisdom of the tribals of three western districts of West Bengal, India against poisonous and non-poisonous bites and stings, a quantitative approach was adopted. These age-old yet unexplored knowledge can be utilized in finding lead-molecules against poisonous and non-poisonous animal-bites. Further, an evidence-based approach is needed to assess the venom-neutralization ability of plants by experimental studies. Read More

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January 2021

Molecular modeling approach to identify inhibitors of Rv2004c (rough morphology and virulent strain gene), a DosR (dormancy survival regulator) regulon protein from .

J Biomol Struct Dyn 2020 Nov 13:1-16. Epub 2020 Nov 13.

Structural Bioinformatics Unit, Department of Biochemistry, Shivaji University, Kolhapur, India.

Being a part of dormancy survival regulator (DosR) regulon, Rv2004c (rough morphology and virulent strain gene) has been identified in earlier experimental studies as an indispensable protein required for the growth and survival of . This protein was predicted to have a role in inhibition of phospholipase A2 activity related to immuno-defence and other membrane-related events. Thus, considering significance of Rv2004c protein, a structure-based drug designing strategy was followed to identify potential inhibitors to this novel target. Read More

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November 2020

Novel variants in PNPLA6 causing syndromic retinal dystrophy.

Exp Eye Res 2021 01 22;202:108327. Epub 2020 Oct 22.

Department of Ophthalmology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China. Electronic address:

PNPLA6-related disorders include several phenotypes, such as Boucher-Neuhäuser syndrome, Gordon Holmes syndrome, spastic paraplegia, photoreceptor degeneration, Oliver-McFarlane syndrome and Laurence-Moon syndrome. In this study, detailed clinical evaluations and genetic testing were performed in five (4 Chinese and 1 Caucasian/Chinese) syndromic retinal dystrophy patients. Genotype-phenotype correlations were analyzed based on review of the literatures of previously published PNPLA6-related cases. Read More

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January 2021

Au-coated FeO core-shell nanohybrids with photothermal activity for point-of-care immunoassay for lipoprotein-associated phospholipase A on a digital near-infrared thermometer.

Anal Bioanal Chem 2021 Jan 13;413(1):235-244. Epub 2020 Oct 13.

Department of Urology, Jinjiang Municipal Hospital, No. 392, Xinhua Street, Jinjiang City, 362200, Fujian, China.

A portable photothermal immunoassay based on Au-coated magnetic FeO core-shell nanohybrids (Au-FeO) was developed for point-of-care (POC) testing of lipoprotein-associated phospholipase A (Lp-PLA) on a digital near-infrared (NIR) thermometer. Au-FeO photothermal materials were first synthesized through reverse micelle method, and then functionalized with polyclonal rabbit anti-human Lp-PLA antibody. A sandwiched immunoreaction was carried out in polyclonal mouse anti-human Lp-PLA antibody-coated microplate using Au-FeO-labeled antibody as the detection antibody. Read More

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January 2021

Galleria mellonella larvae fat body disruption (Lepidoptera: Pyralidae) caused by the venom of Habrobracon brevicornis (Hymenoptera: Braconidae).

Arch Insect Biochem Physiol 2021 Jan 7;106(1):e21746. Epub 2020 Oct 7.

Laboratory of Insect Pathology, Institute of Systematics and Ecology of Animals, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.

The ability of Habrobracon brevicornis venom to elevate the nutritional suitability of a host by affecting the host larvae fat body condition was studied. To understand whether H. brevicornis crude venom impacts the host biochemical profile, the concentrations of total lipids and main sugars in the host larvae lymph were analyzed. Read More

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January 2021

The allosteric activation mechanism of a phospholipase A-like toxin from Bothrops jararacussu venom: a dynamic description.

Sci Rep 2020 10 1;10(1):16252. Epub 2020 Oct 1.

Departamento de Biofísica e Farmacologia, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brasil.

The activation process of phospholipase A-like (PLA-like) toxins is a key step in their molecular mechanism, which involves oligomeric changes leading to the exposure of specific sites. Few studies have focused on the characterization of allosteric activators and the features that distinguish them from inhibitors. Herein, a comprehensive study with the BthTX-I toxin from Bothrops jararacussu venom bound or unbound to α-tocopherol (αT) was carried out. Read More

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October 2020

PLA2R antibody, PLA2R rs4664308 polymorphism and PLA2R mRNA levels in Tunisian patients with primary membranous nephritis.

PLoS One 2020 1;15(10):e0240025. Epub 2020 Oct 1.

Research Laboratory in Immunology of Renal Transplantation and Immunopathology (LR03SP01), Charles Nicolle Hospital, Tunis El Manar University, Tunis, Tunisia.

Background: Primary membranous nephritis (PMN) is an autoimmune disease induced by the deposit of antibodies (Ab) to the phospholipase receptor A2 receptor (PLA2R) on podocytes. In this context, we aimed to assess the relationships between anti-PLA2R Ab, PLA2R rs4664308 SNP, PLA2R mRNA levels and PMN susceptibility and outcome.

Methods: Sixty-eight PMN patients, 30 systemic lupus erythematosus (SLE) patients with secondary MN and 30 healthy control subjects served for anti-PLA2R Ab measurement by ELISA and PLA2R rs4664308 SNP genotyping by a commercial real-time PCR. Read More

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November 2020

A prognosis-predictive nomogram of ovarian cancer with two immune-related genes: and .

Oncol Lett 2020 Nov 8;20(5):204. Epub 2020 Sep 8.

Department of Gynecology of Traditional Chinese Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510000, P.R. China.

Ovarian carcinoma (OV) is one of the most lethal gynecological malignancies globally, and the overall 5-year survival rate of OV was 47% in 2018 according to American data. To increase the survival rate of patients with OV, many researchers have sought to identify biomarkers that act as both prognosis-predictive markers and therapy targets. However, most of these have not been suitable for clinical application. Read More

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November 2020

Antibiofilm Activity of Acidic Phospholipase Isoform Isolated from Snake Venom.

Toxins (Basel) 2020 09 20;12(9). Epub 2020 Sep 20.

Postgraduate Program in Cellular and Molecular Biology-Federal University of Paraíba, João Pessoa, PB 58051-900, Brazil.

Introduction: Bacterial resistance is a worldwide public health problem, requiring new therapeutic options. An alternative approach to this problem is the use of animal toxins isolated from snake venom, such as phospholipases A (PLA), which have important antimicrobial activities. is one of the snake species in the northeast of Brazil that attracts great medical-scientific interest. Read More

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September 2020

Flourensia fiebrigii S.F. blake: A medicinal plant from the Argentinean highlands with potential use as anti-rheumatic and anti-inflammatory.

J Ethnopharmacol 2021 Jan 22;264:113296. Epub 2020 Aug 22.

Laboratorio de Investigación de Productos Naturales (LIPRON), Instituto de Bioprospección y Fisiología Vegetal (INBIOFIV-CONICET), Facultad de Ciencias Naturales e IML, Universidad Nacional de Tucumán, San Lorenzo 1469, San Miguel de Tucumán, Tucumán, Argentina; Instituto de Morfología Vegetal. Área Botánica. Fundación Miguel Lillo, Miguel Lillo 251, San Miguel de Tucumán, Tucumán, Argentina. Electronic address:

Ethnopharmacological Relevance: Flourensia fiebrigii is a plant used in traditional medicine in the Argentine Calchaquí Valley as purgative, expectorant, anti-rheumatic and anti-inflammatory.

Aim Of The Study: The aim of this study was to analyze the macroscopic and microscopic characteristics of F. fiebrigii leaf and stem, the phytochemical composition of leaves ethanolic extracts and to validate its traditional use as anti-rheumatic and anti-inflammatory. Read More

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January 2021

Cellular and Extracellular Proteome of the Animal Pathogen , a Close Relative of Zoonotic and .

Proteomes 2020 Aug 12;8(3). Epub 2020 Aug 12.

Microbiology Division, Department of Biology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany.

is a newly described animal pathogen, closely related to the emerging human pathogen and , a major pathogen of small ruminants. In this study, proteins of a whole cell and a shaving fraction and the exoproteome of strain W25 were analyzed as a first proteome study of this species. In total, 1305 proteins were identified out of 2013 proteins encoded by the W25 genome sequence and number of putative virulence factors were detected already under standard growth conditions including phospholipase D and sialidase. Read More

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Mutational analysis on predicting the impact of high-risk SNPs in human secretary phospholipase A2 receptor (PLA2R1).

Sci Rep 2020 07 16;10(1):11750. Epub 2020 Jul 16.

Department of Biology, College of Science, University of Jeddah, Jeddah, Saudi Arabia.

PLA2R1 is a transmembrane glycoprotein that acts as an endogenous ligand which stimulates the processes including cell proliferation and cell migration. The SNPs in PLA2R1 is associated with idiopathic membranous nephropathy which is an autoimmune kidney disorder. The present study aimed to explore the structure-function analysis of high risk SNPs in PLA2R1 by using 12 different computational tools. Read More

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Characterization of KPC-Producing in an Intensive Care Unit of a Brazilian Tertiary Hospital.

Front Microbiol 2020 20;11:956. Epub 2020 May 20.

Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, Brazil.

has emerged as an important opportunistic pathogen responsible for nosocomial and severe infections. Here, we determined phenotypic and molecular characteristics of 54 isolates obtained from patient samples from intensive-care-unit (ICU) and neonatal intensive-care-unit (NIUC) of a Brazilian tertiary hospital. All isolates were resistant to beta-lactam group antibiotics, and 92. Read More

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Ethyl esters production catalyzed by immobilized lipases is influenced by n-hexane and ter-amyl alcohol as organic solvents.

Bioprocess Biosyst Eng 2020 Nov 27;43(11):2107-2115. Epub 2020 Jun 27.

Department of Catalysis, CSIC/Campus UAM, 28049, Madrid, Spain.

Lipase stability in organic solvent is crucial for its application in many biotechnological processes as biocatalyst. One way to improve lipase's activity and stability in unusual reaction medium is its immobilization on inert supports. Here, lipases from different sources and immobilized through weak chemical interactions on hydrophobic and ionic supports had their transesterification ability dramatically dependent on the support and also on the solvent that had been used. Read More

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November 2020

Putative Phospholipase B-Like 2 is Not Responsible for Polysorbate Degradation in Monoclonal Antibody Drug Products.

J Pharm Sci 2020 09 11;109(9):2710-2718. Epub 2020 Jun 11.

Preclinical Manufacture & Process Development, Regeneron Pharmaceuticals, Tarrytown, New York 10591.

Polysorbates (PS) are surfactants commonly added in a therapeutic protein drug product as excipients to protect proteins from denaturation and aggregation during storage, transportation, and delivery. Significant degradation of PS in drug products could lead to shortened drug shelf lives and PS-degrading activity in drug products must be minimized. Identification of lipases that degrade PS could lead to better process control in drug manufacturing. Read More

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September 2020

In vivo and in vitro Pathogenesis and Virulence Factors of Candida albicans Strains Isolated from Cutaneous Candidiasis.

Iran Biomed J 2020 09 19;24(5):324-32. Epub 2020 Feb 19.

Department of Medical Mycology, Pasteur Institute of Iran, Tehran 1316943551, Iran.

Background: The Candida albicans is one of the most important global opportunistic pathogens, and the incidence of candidiasis has increased over the past few decades. Despite the established role of skin in defense against fungal invasion, little has been documented about the pathogenesis of Candida species when changing from normal flora to pathogens of vaginal and gastrointestinal epithelia. This study was carried out to determine the in vivo and in vitro pathogenesis of clinical C. Read More

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September 2020

Crotamine in : distribution according to subspecies and geographic origin, in captivity or nature.

J Venom Anim Toxins Incl Trop Dis 2020 Apr 6;26:e20190053. Epub 2020 Apr 6.

Laboratory of Herpetology, Butantan Institute, São Paulo, SP, Brazil.

Background: is considered one of the most important species of venomous snakes in Brazil, due to the high mortality of its snakebites. The venom of contains four main toxins: crotoxin, convulxin, gyroxin and crotamine. Venoms can vary in their crotamine content, being crotamine-negative or -positive. Read More

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