29,026 results match your criteria phosphate buffer


Electrochemical detection of 2-nitrophenol with BaO nanorods modified glassy carbon electrode.

Chem Asian J 2021 Apr 13. Epub 2021 Apr 13.

King Abdulaziz University, Chemistry, SAUDI ARABIA.

Here, an electrochemical detection approach (differential pulse voltammetry) was employed to develop 2-nitrophenol (2-NP) sensor probe using glassy carbon electrode (GCE) coated by wet-chemically synthesized nanorods (NRs) of BaO. The prepared BaO NRs were totally characterized by Field emission scanning electron microscopy (FESEM), Energy-dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS) and powder X-ray diffraction (XRD) analysis. The peak currents by differential pulse voltammetric (DPV) analysis of 2-NP are plotted against the concentration to obtain the calibration curve of the 2-NP detection. Read More

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Vivianite scaling in wastewater treatment plants: Occurrence, formation mechanisms and mitigation solutions.

Water Res 2021 Mar 14;197:117045. Epub 2021 Mar 14.

Dept. Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, Netherlands.

The presence of soluble iron and phosphorus in wastewater sludge can lead to vivianite scaling. This problem is not often reported in literature, most likely due to the difficult identification and quantification of this mineral. It is usually present as a hard and blue deposit that can also be brown or black depending on its composition and location. Read More

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Narrow pH tolerance found for a microbial fuel cell treating winery wastewater.

J Appl Microbiol 2021 Apr 12. Epub 2021 Apr 12.

School of Engineering, The University of British Columbia Okanagan, British Columbia, Canada.

Aims: The use of microbial fuel cells (MFC) to treat winery wastewater is promising; however, an initial acidic pH, fluctuating chemical oxygen demand (COD) levels and a lack of natural buffering in these wastewaters makes MFC operation challenging. This study addresses these challenges by providing a suitable buffer system at an ideal buffer to COD ratio.

Methods And Results: A lab scale MFC was designed, inoculated with anaerobic winery sludge, and fed with synthetic winery wastewater. Read More

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Opposite Effects of Potassium Ions on the Thermal Stability of i-Motif DNA in Different Buffer Systems.

Authors:
Bo Gao Xi-Miao Hou

ACS Omega 2021 Apr 24;6(13):8976-8985. Epub 2021 Mar 24.

College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.

i-motifs are noncanonical DNA structures formed the stack of intercalating hemi-protonated C: C base pairs in C-rich DNA strands and play essential roles in the regulation of gene expression. Here, we systematically investigated the impacts of K on i-motif DNA folding using different buffer systems. We found that i-motif structures display very different values at the same pH and ion strength in different buffer systems. Read More

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A Paper-Based Electrochemical Sensor For Ho Detection In Aerosol Phase: Measure Of Ho Nebulized By A Reconverted Ultrasonic Aroma Diffuser As A Case Of Study.

Microchem J 2021 Apr 5:106249. Epub 2021 Apr 5.

Department of Chemical Science and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, Rome, 00133, Italy.

The outbreak of COVID-19 is caused by high contagiousness and rapid spread of SARS-CoV-2 virus between people when an infected person is in close contact with another one. In this overall scenario, the disinfection processes have been largely improved. For instance, some countries have approved no-touch technologies by vaporizing disinfectants such as hydrogen peroxide, with the overriding goal to boost the safety of the places. Read More

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Ferrate Self-Decomposition in Water Is Also a Self-Activation Process: Role of Fe(V) Species and Enhancement with Fe(III) in Methyl Phenyl Sulfoxide Oxidation by Excess Ferrate.

Water Res 2021 Mar 29;197:117094. Epub 2021 Mar 29.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.

To reveal the role of ferrate self-decomposition and the fates of intermediate iron species [Fe(V)/Fe(IV) species] during ferrate oxidation, the reaction between ferrate and methyl phenyl sulfoxide (PMSO) at pH 7.0 was investigated as a model system in this study. Interestingly, the apparent second-order rate constants (k) between ferrate and PMSO was found to increase with ferrate dosage in the condition of excess ferrate in borate buffer. Read More

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[microRNA-1 gene delivery mediated by exosomes suppresses CAL-27 cell proliferation].

Hua Xi Kou Qiang Yi Xue Za Zhi 2021 Apr;39(2):136-142

Orofacial Reconstruction and Regeneration Laboratory, School of Stomatology of Southwest Medical University, Luzhou 646000, China.

Objectives: This study aims to construct endogenous exosomes abundantly loaded with miR-1 and investigate the role of exosome-mediated microRNA-1 (miR-1) delivery on CAL-27 cell proliferation.

Methods: Exosomes secreted by miR-1-overexpressing HEK293 cells (miR1-EXO) were purified via ultracentrifugation and subjected to transmission electron microscopy, nanoparticle analysis, Western blot analysis, and quantitative polymerase chain reaction (qPCR). CAL-27 cells were cocultured with exosomes secreted by HEK293 cells (CON-EXO) and miR1-EXO and equivalent phosphate buffer saline. Read More

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Design, Preparation and evaluation of various parameters of controlled release matrices of losartan potassium using polymers combination.

Pak J Pharm Sci 2020 Sep;33(5(Supplementary)):2231-2237

Faculty of Pharmacy and Health Sciences, University of Balochistan, Quetta, Pakistan.

Controlled release formulations are administered once a day and reduce frequency of dose and ensuring patient's compliance. In the current research controlled release matrices of losartan potassium formulated with polymeric combinations of ethocel grade 7 with carbopol 934P NF using different concentrations of polymers. In some polymeric tablets, Co-excipients like CMC, Starch, HPMC was added by replacing of 10% of filler in formulations at 10:5. Read More

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September 2020

[Effects of Acrolein on the Proliferation of and 1 Gene Expression in Pulmonary Epithelial Cells].

Sichuan Da Xue Xue Bao Yi Xue Ban 2021 Mar;52(2):216-221

Department of Occupational and Environmental Health, School of Public Health, Xinxiang Medical University, Xinxiang 453003, China.

Objective: To investigate the effect of acrolein on the proliferation of pulmonary epithelial cells and its possible mechanism.

Methods: Two strains of pulmonary epithelial cells, A549 cells and MLE15 cells, were used as models of pulmonary epithelial cell, and were treated with 80 μmol/L acrolein or phosphate buffer saline (PBS) as the control. The proliferation of pulmonary epithelial cells were determined with CCK-8 kit after cell culturing resumed for 12 h, 24 h, 36 h and 48 h post acrolein treatment, and the expression of period circadian regulator gene 1 ( 1) was examined using Western blot test 24 h after acrolein treatment. Read More

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An innovative and cost-effective way to estimate alkaline phosphatase activity in cellular model systems.

Int J Biochem Mol Biol 2021 15;12(1):1-7. Epub 2021 Feb 15.

Department of Biochemistry, Postgraduate Institute of Medical Education and Research Chandigarh 160012, India.

Alkaline phosphatase is an enzyme that converts para-nitrophenyl phosphate to para-nitrophenol (yellow coloured) in 2-amino, 2-methyl, 1-propanol buffer at pH 10.5. However, when this protocol is applied to the in vitro cellular model systems to estimate alkaline phosphatase activity, it tends to generate clumps of genomic DNA, leading to inaccurate pipetting for protein estimation. Read More

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February 2021

Quality-by-Design Approach for Chromatographic Analysis of Metformin, Empagliflozin and Linagliptin.

J Chromatogr Sci 2021 Apr 5. Epub 2021 Apr 5.

Gokaraju Rangaraju College of Pharmacy, Osmania University, Hyderabad 500090, India.

New analytical quality by design-oriented HPLC method with multiple response optimization (Derringer's desirability function) was demonstrated for simultaneous analysis of three antidiabetic drugs (metformin hydrochloride/empagliflozin/linagliptin) in a fixed-dose combination. Central composite design was employed for systematic optimization of critical method parameters, namely, % organic phase (X1), aqueous phase pH (X2) and flow rate (X3) while resolution, capacity factor and theoretical plate number as critical analytical attributes. Effective chromatographic separation of title analytes was accomplished on Std. Read More

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Ultrasonic measurement of dentin remineralization effects of dentifrices and silver diamine fluoride.

Acta Odontol Scand 2021 Apr 6:1-8. Epub 2021 Apr 6.

Department of Operative Dentistry, Nihon University School of Dentistry, Tokyo, Japan.

Objective: This study investigated the dentin remineralization effect of the application of a functionalized tri-calcium phosphate (fTCP) dentifrice and a silver diamine fluoride (SDF) solution.

Material And Methods: The materials used were: a fluoride-containing dentifrice with fTCP (fTCP+), a fluoride-containing dentifrice without fTCP (fTCP-) and a 38% SDF solution. Following treatment, the dentin slabs were immersed in a 0. Read More

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Immune Response and Hemolymph Microbiota of Apis mellifera and Apis cerana After the Challenge With Recombinant Varroa Toxic Protein.

J Econ Entomol 2021 Apr 2. Epub 2021 Apr 2.

Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, Guangdong, China.

The honey bee is a significant crop pollinator and key model insect for understanding social behavior, disease transmission, and development. The ectoparasitic Varroa destructor mite put threats on the honey bee industry. A Varroa toxic protein (VTP) from the saliva of Varroa mites contributes to the toxicity toward Apis cerana and the deformed wing virus elevation in Apis mellifera. Read More

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A method for detection of SARS-CoV-2 RNA in healthy human stool: a validation study.

Lancet Microbe 2021 Mar 31. Epub 2021 Mar 31.

Division of Bacterial, Parasitic and Allergenic Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, MD, USA.

Background: Faecal shedding of SARS-CoV-2 has raised concerns about transmission through faecal microbiota transplantation procedures. Validation parameters of authorised tests for SARS-CoV-2 RNA detection in respiratory samples are described in product labelling, whereas the published methods for SARS-CoV-2 detection from faecal samples have not permitted a robust description of the assay parameters. We aimed to develop and validate a test specifically for detection of SARS-CoV-2 in human stool. Read More

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MACROMICROSCOPIC ARGUMENTATION OF THE PATHOGENETIC SCENARIO OF BABESIOSIS IN THE COORDINATE SYSTEM «PATHOGEN-CARRIER-RESERVOIR».

Authors:
Inna I Torianyk

Wiad Lek 2021 ;74(3 cz 1):436-440

STATE INSTITUTION «MECHNIKOV INSTITUTE OF MICROBIOLOGY AND IMMUNOLOGY OF THE NATIONAL ACADEMY OF MEDICAL SCIENCES OF UKRAINE», KHARKIV, UKRAINE.

Objective: The aim is to get a thorough argument for the babesiosis pathogenetic scenario in the coordinate system «pathogen (Babesia spp.) - carrier (ticks of the Ixodoidea superfamily of the Ixodidea family) - reservoir (a susceptible organism)» with the emphasis on the epizootic/epidemic role of the carrier.

Patients And Methods: Materials and methods: The macromicroscopic method of research was used in order to maximize the clarification of the babesiosis scenario, its pathogenetic links, the connection of the latter with attacks of active stages of ixodes ticks, types of circulation of ontogenetic forms of Babesia spp. Read More

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Coating characterization by hyperspectroscopy and predictive dissolution models of tablets coated with blends of cellulose acetate and cellulose acetate phthalate.

AAPS PharmSciTech 2021 Apr 2;22(3):122. Epub 2021 Apr 2.

Irma Lerma Rangel College of Pharmacy, Texas A&M Health Science Center, Texas A&M University, 310 Reynolds Medical Sciences Building, College Station, Texas, 77843-1114, USA.

The objective of current research was to develop the models of dissolution prediction of tablets coated with cellulose acetate (CA 320S or CA 398-10) and cellulose acetate phthalate (C-A-P) blends. Independent variables selected were coating percent (X) and percent of CA 320S or CA 398-10 (X) in the blend. Dependent variables selected were dissolution in 1 (Y), 8 (Y), and 24 h (Y). Read More

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Electrospun Biomaterials from Chitosan Blends Applied as Scaffold for Tissue Regeneration.

Polymers (Basel) 2021 Mar 26;13(7). Epub 2021 Mar 26.

Biomaterials Applications, 6 Rue Lesdiguières, 38000 Grenoble, France.

Our objective in this work was to summarize the main results obtained in processing pure chitosan and chitosan/hyaluronan complex in view of biomedical applications, taking advantage of their original properties. In addition, an electrospinning technique was selected to prepare nanofiber mats well adapted for tissue engineering in relation to the large porosity of the materials, allowing an exchange with the environment. The optimum conditions for preparation of purified and stable nanofibers in aqueous solution and phosphate buffer pH = 7. Read More

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Oral Diagnostic Methods for the Detection of Periodontal Disease.

Diagnostics (Basel) 2021 Mar 22;11(3). Epub 2021 Mar 22.

Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, 8032 Zurich, Switzerland.

Periodontitis is a common immune-inflammatory oral disease. Early detection plays an important role in its prevention and progression. Saliva is a reliable medium that mirrors periodontal health and is easily obtainable for identifying periodontal biomarkers in point-of-care diagnostics. Read More

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Microbial Synthesis of ()- and ()-Benzoin in Enantioselective Desymmetrization and Deracemization Catalyzed by Included in the Blossom Protect™ Agent.

Molecules 2021 Mar 12;26(6). Epub 2021 Mar 12.

Department of Medical Biology and Biochemistry, Faculty of Medicine, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, 24 Karłowicz Street, 85-092 Bydgoszcz, Poland.

In this study, we examined the strains DSM 14940 and DSM 14941 included in the Blossom Protect™ agent to be used in the bioreduction reaction of a symmetrical dicarbonyl compound. Both chiral 2-hydroxy-1,2-diphenylethanone antipodes were obtained with a high enantiomeric purity. Mild conditions (phosphate buffer [pH 7. Read More

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Development of a Potent Stabilizer for Long-Term Storage of Foot-and-Mouth Disease Vaccine Antigens.

Vaccines (Basel) 2021 Mar 12;9(3). Epub 2021 Mar 12.

Animal and Plant Quarantine Agency, Gimcheon 39660, Gyeonsangbuk-do, Korea.

A local virus isolate, O/SKR/JC/2014 (O JC), has been considered as a candidate vaccine strain in the development of a domestic foot-and-mouth disease (FMD) vaccine in Korea. However, producing and preserving a sufficient quantity of intact vaccine antigens from the O JC strain was difficult owing to its distinctive structural instability compared to other candidate vaccine strains. Based on this feature, the O JC strain was adopted as a model virus for the stabilization study to determine the optimal stabilizer composition, which enables long-term storage of the FMD vaccine antigen in both aqueous and frozen phases. Read More

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Resuscitation of Pulsed Electric Field-Treated and in a Rich Nutrient Medium.

Foods 2021 Mar 19;10(3). Epub 2021 Mar 19.

Department of Chemical Engineering, Ariel University, Ariel 40700, Israel.

Pulsed electric fields (PEFs) technology was reported to be useful as a disinfection method in the liquid food industry. This technology may lead to membrane permeabilization and bacterial death. However, resuscitation of viable but non-culturable cells and sublethally injured microorganisms in food was reported to be associated with foodborne outbreaks. Read More

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Ionic Cross-Linking as a Strategy to Modulate the Properties of Oral Mucoadhesive Microparticles Based on Polysaccharide Blends.

Pharmaceutics 2021 Mar 19;13(3). Epub 2021 Mar 19.

School of Pharmaceutical Science, São Paulo State University (UNESP), Araraquara, Road Araraquara-Jaú, Km 01, Araraquara, São Paulo 14801-902, Brazil.

Polymer blends of gellan gum (GG)/retrograded starch(RS) and GG/pectin (P) were cross-linked with calcium, aluminum, or both to prepare mucoadhesive microparticles as oral carriers of drugs or nano systems. Cross-linking with different cations promoted different effects on each blend, which can potentially be explored as novel strategies for modulating physical-chemical and mucoadhesive properties of microparticles. Particles exhibited spherical shapes, diameters from 888 to 1764 µm, and span index values lower than 0. Read More

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Silencing of Affects Maturation Pathways in Mouse Neonatal Cardiomyocytes.

Int J Mol Sci 2021 Mar 31;22(7). Epub 2021 Mar 31.

Department of Internal and Agricultural Medicine, Faculty of Medicine, Jagiellonian University Medical College, 31-121 Cracow, Poland.

Sphingosine kinase-1 (Sphk1) and its product, sphingosine-1-phosphate (S1P) are important regulators of cardiac growth and function. Numerous studies have reported that Sphk1/S1P signaling is essential for embryonic cardiac development and promotes pathological cardiac hypertrophy in adulthood. However, no studies have addressed the role of Sphk1 in postnatal cardiomyocyte (CM) development so far. Read More

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A Comparison of Porphyrin Photosensitizers in Photodynamic Inactivation of RNA and DNA Bacteriophages.

Viruses 2021 Mar 23;13(3). Epub 2021 Mar 23.

Department of Biomedical Sciences, University of Wisconsin-Milwaukee, 2400 E. Hartford Ave., Milwaukee, WI 53211, USA.

Effective broad-spectrum antiviral treatments are in dire need as disinfectants and therapeutic alternatives. One such method of disinfection is photodynamic inactivation, which involves the production of reactive oxygen species from dissolved oxygen in response to light-stimulated photosensitizers. This study evaluated the efficacy of functionalized porphyrin compounds for photodynamic inactivation of bacteriophages as human virus surrogates. Read More

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Structural Polymorphism of Sorafenib Tosylate as a Key Factor in Its Solubility Differentiation.

Pharmaceutics 2021 Mar 13;13(3). Epub 2021 Mar 13.

Department of Pharmacognosy, Faculty of Pharmacy, Poznań University of Medical Sciences, Święcickiego 4, 60-781 Poznań, Poland.

The presence of active pharmaceutical ingredients (APIs) in the forms of different polymorphic states can induce differences in their physicochemical properties. In the case of poorly soluble APIs, like the oncological drug sorafenib tosylate, small variations in solubility may result in large bioavailability differences. The control of its therapeutic dose is crucial from the effective pharmacotherapy point of view and the reduction of side effects. Read More

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