50 results match your criteria phosphatase esterase-lipase


Phenotypic characterization of indigenous Saccharomyces cerevisiae strains associated with sorghum beer and palm wines.

World J Microbiol Biotechnol 2021 Jan 11;37(2):24. Epub 2021 Jan 11.

Centre de Coopération Internationale en Recherche Agronomique pour le Développement, CIRAD, UMR Qualisud, TA 95B/16, 34398, Montpellier Cedex 5, France.

In order to phenotypically characterized Saccharomyces cerevisiae strains isolated from sorghum beer and palm wines for a possible selection of a starter culture, 30 strains were tested for killer activity, temperature resistance, ethanol tolerance, carbohydrate fermentation, enzyme profile and sorghum wort fermentation. Of the tested strains, three showed a killer profile, while four showed a neutral profile and 23 were found to be sensitive to K2 toxin. Temperatures of 40 °C and 44 °C allowed to distinguish strains into four thermal groups with only three strains may grow at 44 °C. Read More

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January 2021

Genotypic and Phenotypic Characterization of from Bovine Digital Dermatitis.

Microorganisms 2020 Oct 2;8(10). Epub 2020 Oct 2.

Sunchon National University, Suncheon, Jeonnam 57922, Korea.

This study aimed to isolate and characterize spp. from bovine digital dermatitis (BDD)-infected dairy cattle. Seven isolates were characterized in this study. Read More

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October 2020

Aliarcobacter vitoriensis sp. nov., isolated from carrot and urban wastewater.

Syst Appl Microbiol 2020 Jul 19;43(4):126091. Epub 2020 May 19.

Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy. University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain.

Two isolates, one recovered from a carrot and another one from urban wastewater, were characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both isolates clustered together, and were most closely related to Aliarcobacter lanthieri. Multilocus phylogenetic analysis (MLPA) using the concatenated sequences of five housekeeping genes (atpA, gyrA, gyrB, hsp60 and rpoB) suggested that these isolates formed a distinct phylogenetic lineage among the genera derived from the former genus Arcobacter. Read More

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Anticandidal activity of Lactobacillus spp. in the presence of galactosyl polyols.

Microbiol Res 2020 Nov 27;240:126540. Epub 2020 Jun 27.

Institute of Fermentation Technology and Microbiology, Łódź University of Technology, Wolczanska 171/173, 90-024, Łódź, Poland. Electronic address:

Yeasts have a substantial impact on the contamination and loss of food. In this study, we applied bacteria of the genus Lactobacillus as natural biopreservatives. Anticandidal strains of bacteria were selected from among 60 strains of bacteria grown which were each with nine polyols or galactosyl polyols. Read More

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November 2020

Physiological and genome-wide RNA-sequencing analyses identify candidate genes in a nitrogen-use efficient potato cv. Kufri Gaurav.

Plant Physiol Biochem 2020 Sep 9;154:171-183. Epub 2020 Jun 9.

ICAR-Indian Agricultural Statistics Research Institute, New Delhi, 110012, India.

Nitrogen (N) is an important nutrient for plant growth. However, its excess application leads to environmental damage. Hence, improving nitrogen use efficiency (NUE) of plant is one of the plausible options to solve the problems. Read More

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September 2020

Candida species in tchapalo and bangui, two traditional alcoholic beverages from Côte d'Ivoire.

Fungal Biol 2018 05 2;122(5):283-292. Epub 2018 Feb 2.

Nangui Abrogoua University, Laboratory of Biotechnology and Food Microbiology, Department of Food Science and Technology (UFR-STA), 02 BP 801 Abidjan 02, Cote d'Ivoire.

The increase of infections due to non-Candida albicans species made it very necessary to conduct adequate characterization to be able to identify the species of Candida isolated from traditional fermented foods. In this study, based on their hue on Candida Chromogenic Agar medium, a total of 136 yeast strains were isolated from tchapalo and bangui. Molecular identification based on PCR-RFLP of internal transcribed spacers of rDNA (ITS) and sequencing of the ITS and the D1/D2 regions allowed us to assign these isolates to seven species: Candida tropicalis, Candida inconspicua, Candida rugosa, Saccharomyces cerevisiae, Kluyveromyces marxianus, Hanseniaspora guilliermondii, Trichosporon asahii. Read More

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Fungi of the genus Rhodotorula isolated from the oral cavity of oncologic patients with colorectal cancer

Ann Parasitol 2017 ;63(1):57-62

This paper concerns the frequency of occurrence and enzymatic activity of fungi of the genus Rhodotorula isolated from the oral cavity of patients with colorectal cancer. Swabs from the oral cavity were subjected to standard mycological diagnostic procedures; enzymatic activity of the fungi was evaluated using and API Zym test by BioMereux. The fungi of the genus Rhodotorula were noted in 22. Read More

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January 2017

Probiotic Characteristics of Lactobacillus curvatus DN317, a Strain Isolated from Chicken Ceca.

Probiotics Antimicrob Proteins 2017 12;9(4):415-424

Unité de Protéomique Fonctionnelle et Potentiel Nutraceutique de la Biodiversité de Tunisie, Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université de Tunis El-Manar, 1006, Tunis, Tunisia.

The probiotic characteristics of Lactobacillus curvatus DN317, a strain isolated from chicken ceca, were evaluated. This strain was selected for study from the isolated Lactobacillus strains because it has specific anti-microbial activity against Campylobacter jejuni ATCC 33560, Camp. jejuni NCTC 11168, Listeria monocytogenes ATCC 7644, and Bacillus subtilis ATCC 8633. Read More

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December 2017

Faucicola mancuniensis gen. nov., sp. nov., isolated from the human oropharynx.

Int J Syst Evol Microbiol 2015 Jan 29;65(Pt 1):11-14. Epub 2014 Sep 29.

Manchester Pharmacy School, Oxford Road, University of Manchester, Manchester M13 9PT, UK.

An aerobic, Gram-stain-negative, non-motile coccus, designated strain GVCNT2(T), was isolated from the tonsils of a healthy adult female. Cells were oxidase- and catalase-positive, positive for the production of esterase (C4), esterase lipase (C8) and leucine arylamidase, and weakly positive for naphthol-AS-BI-phosphohydrolase and alkaline phosphatase. Cells were also capable of hydrolysing DNA. Read More

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January 2015

Selected phenotypic features of BR91, a unique spirochaetal strain isolated from the Culex pipiens mosquito.

Microbiol Res 2014 May-Jun;169(5-6):348-52. Epub 2013 Oct 19.

Institute of Vertebrate Biology, v.v.i., Academy of Sciences of the Czech Republic, Květná 8, 603 65 Brno, Czech Republic; Masaryk University, Department of Experimental Biology, Section of Microbiology, Kotlářská 2, 611 37 Brno, Czech Republic. Electronic address:

Growth temperature range, resistance to selective antibiotics, activities of 23 enzymes, protein fingerprints and fatty acids composition of the spirochaetal strain BR91, isolated from the Culex pipiens mosquito, were tested. The spirochaetes were grown in BSK-H Complete liquid medium. The optimal in vitro growth temperature of the strain was 33 °C. Read More

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November 2014

Enzymatic profiling of clinical and environmental isolates of Burkholderia pseudomallei.

Trop Biomed 2012 Mar;29(1):160-8

Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

Melioidosis has been recognized as an important cause of sepsis in the tropics. The disease caused by an environmental saprophyte Burkholderia pseudomallei, affects mostly adults with underlying immunocompromised conditions. In this study, the enzymatic profiles of 91 clinical and 9 environmental isolates of B. Read More

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Preliminary characterization of the probiotic properties of Candida famata and Geobacillus thermoleovorans.

Iran J Microbiol 2011 Sep;3(3):129-34

Laboratory of Analysis, Treatment and Valorization of Pollutants of the Environment and Products (LATVPEP). Faculty of Pharmacy, University of Monastir, 5000. Tunisia.

Background And Objective: Probiotics are live microbial feed supplements which beneficially affect the host animal by improving its intestinal microbial balance, producing metabolites which inhibit the colonization or growth of other microorganisms or by competing with them for resources such as nutrients or space. The aim of this study was to investigate the probiotic properties of Candida famata and Geobacillus thermoleovorans.

Material And Methods: In this study, yeast and bacterial strains isolated from pure oil waste were identified using Api 50 CHB and Api Candida Systems and their probiotic properties were studied through antimicrobial activity, biofilm production, adherence assay and enzymatic characterization. Read More

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September 2011

Biochemical activities of Brochothrix thermosphacta.

Meat Sci 2012 Feb 25;90(2):410-3. Epub 2011 Aug 25.

Technical University of Lodz, Institute of Fermentation Technology and Microbiology Wolczanska 171/173, 90-924 Lodz, Poland.

Activities of 19 hydrolases were estimated in cell suspensions of 40 Brochothrix thermosphacta strains, isolated from meat and meat products packaged under various conditions, by using API ZYM® test. These strains produced: acidic phosphatase, esterase C4, esterase/lipase C8, α-chymotrypsin, leucine arylamidase, β-glucosidase and α-glucosidase. The predominating biotype produced only the first four of the listed enzymes. Read More

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February 2012

Toxicity of Photobacterium damselae subsp. damselae strains isolated from new cultured marine fish.

Dis Aquat Organ 2010 Oct;92(1):31-40

Department of Microbiology, Faculty of Sciences, University of Malaga, 29071 Malaga, Spain.

The in vivo and in vitro toxicity of bacterial cells and their extracellular products (ECPs) from 16 strains of Photobacterium damselae subsp. damselae isolated from 7 epizootic outbreaks were evaluated. On the basis of their 50% lethal dose (LD50) values (about 1 x 10(50 CFU), these strains may be considered as moderately virulent. Read More

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October 2010

Effects of PAH biodegradation in the presence of non-ionic surfactants on a bacterial community and its exoenzymatic activity.

J Environ Sci Health A Tox Hazard Subst Environ Eng 2010 ;45(4):421-31

Department of Microbiology, Soochow University, Shih-Lin, Taipei, Taiwan.

The influence of two non-ionic surfactants (TX-100 and Brij 35) on a bacterial community and its exoenzymatic activity during polycyclic aromatic hydrocarbon (naphthalene, phenanthrene and pyrene) biodegradation was evaluated in this study. The result indicated the addition of the non-ionic surfactants altered the profiles of the microbial populations and produced exoenzymes. Fluorescence in situ hybridization found that, as PAH biodegradation progressed in the presence of non-ionic surfactant, the proportion of Bacteria presents increased significantly from the range 54. Read More

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Paenibacillus pinihumi sp. nov., a cellulolytic bacterium isolated from the rhizosphere of Pinus densiflora.

J Microbiol 2009 Oct 24;47(5):530-5. Epub 2009 Oct 24.

Biological Resources Center, KRIBB, Daejeon, Republic of Korea.

A novel cellulolytic bacterium, strain S23(T), was isolated from the rhizosphere of the pine trees in Daejeon, Republic of Korea. This isolate was Gram-positive, strictly aerobic, rod-shaped, catalase-negative, oxidase-positive, motile by means of peritrichous flagella, and tested positive for alkaline phosphatase, esterase lipase, leucine arylamidase, alpha-galactosidase, and beta-galactosidase activities. The DNA G+C content was 49. Read More

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October 2009

Inhibitory effect of nicotinamide on enzymatic activity of selected fungal strains causing skin infection.

Mycoses 2010 May 16;53(3):204-7. Epub 2009 Sep 16.

Department of Clinical Microbiology, Polish Mother's Hospital Research Institute, Łódź, Poland.

Pathogenicity of fungi is connected with their ability to easily penetrate the host tissues, survive in the infected host organism and use the elements of the host tissues as nutrients. Hence, the co-occurrence of pathogenic properties with the high enzymatic activity, which is manifested through the production of various enzymes including extracellular enzymes, was observed. It can be expected that it is possible to decrease fungal pathogenicity by lowering their enzymatic activity. Read More

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Marinobacterium maritimum sp. nov., a marine bacterium isolated from Arctic sediment.

Int J Syst Evol Microbiol 2009 Dec 30;59(Pt 12):3030-4. Epub 2009 Jul 30.

Department of Microbiology, Chungbuk National University, 12 Gaeshin-dong, Cheongju 361-763, Republic of Korea.

A Gram-negative, aerobic, rod-shaped, motile, marine bacterium, strain AR11(T), was isolated from Arctic marine sediment. Strain AR11(T) grew with 0.5-7 % NaCl and at 7-37 degrees C and pH 5. Read More

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December 2009

Spongiibacter marinus gen. nov., sp. nov., a halophilic marine bacterium isolated from the boreal sponge Haliclona sp. 1.

Int J Syst Evol Microbiol 2008 Mar;58(Pt 3):585-90

Berlin University of Technology, Environmental Microbiology Group, Franklinstrasse 29, Sekr. FR 1-2, D-10587 Berlin, Germany.

Strain HAL40b(T) was isolated from the marine sponge Haliclona sp. 1 collected at the Sula Ridge off the Norwegian coast and characterized by physiological, biochemical and phylogenetic analyses. The isolate was a small rod with a polar flagellum. Read More

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Proteolytic activity in salivary gland products of sheep bot fly (Oestrus ovis) larvae.

Vet Parasitol 2007 Oct 13;149(1-2):117-25. Epub 2007 Aug 13.

Centro de Investigaciones Biológicas del Noroeste, Mar Bermejo 195, Col. Playa de Santa Rita, La Paz, B.C.S. 23090, Mexico.

This study identified and characterized hydrolytic enzymes in salivary gland products of Oestrus ovis larvae. Third instars were collected from the heads of slaughtered goats. Salivary glands were extracted, their products obtained by centrifugation and the enzymatic profile determined. Read More

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October 2007

Extracellular enzymatic activities in Cryptococcus neoformans strains isolated from AIDS patients in different countries.

Rev Iberoam Micol 2006 Dec;23(4):216-20

Dipartimento Discipline Medico-Chirurgiche, Sezione Malattie Infettive, Università di Torino, C.so Svizzera 164, 10.140 Torino, Italy.

Three hundred and ten Cryptococcus neoformans strains isolated from AIDS patients in five different countries (151 from Brazil, 23 from Italy, 28 from Spain, 104 from Thailand and four from Turkey) were tested by the API-ZYM kit to detect their extracellular enzymatic activity. The enzymes esterase (C4) (no 3), esterase lipase (C8) (no 4), leucine arylamidase (no 6) and acid phosphatase (no 11) were commonly positive in most of the strains (more than 95%). These enzymes could be considered a useful tool not only for C. Read More

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December 2006

Hydrolase activity in the venom of the pupal endoparasitic wasp, Pimpla hypochondriaca.

Comp Biochem Physiol B Biochem Mol Biol 2005 Jul;141(3):373-81

Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK.

Venom from the pupal endoparasitoid, Pimpla hypochondriaca has previously been shown to contain a mixture of biologically active molecules. Currently, P. hypochondriaca venom was examined for the presence of hydrolase activity. Read More

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Extracellular enzymatic activity and serotype of Cryptococcus neoformans strains isolated from AIDS patients in Brazil.

Rev Iberoam Micol 2005 Mar;22(1):29-33

Laboratorio Micología Médica, Dipartimento Discipline Medico-Chirurgiche, Sezione Malattie Infettive, Università di Torino Corso, Turin, Italy.

One hundred and fifty-one Cryptococcus neoformans strains isolated from AIDS patients in Brazil and maintained in the Adolfo Lutz Institute (São Paulo, Brazil) were tested for phospholipase, protease and other extracellular enzymatic activities and their serotypes determined. Production of extracellular phospholipase and protease was tested by the agar plate methods. Determination of extracellular enzyme profile of the strains was performed by using the API-ZYM kit system, which can test 19 different enzymes. Read More

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Identification of allergens in the venom of the common striped scorpion.

Ann Allergy Asthma Immunol 2004 Nov;93(5):493-8

Department of Allergy and Immunology, Wilford Hall USAF Medical Center, Lackland AFB, San Antonio, Texas, USA.

Background: The common striped scorpion, Centruroides vittatus, is endemic to the southwestern United States and causes thousands of human stings annually. Immediate hypersensitivity reactions to C. vittatus venom have been reported. Read More

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November 2004

[Biochemical properties of Corynebacterium amycolatum strains].

Med Dosw Mikrobiol 2004 ;56(2):147-54

Katedra i Zakład Mikrobiologii, Akademia Medyczna im. L. Rydygiera w Bydgoszczy.

C. amycolatum is the most commonly isolated nonlipophilic species of Corynebacterium from clinical samples. However, the lack of good commercial identification tests in microbiology laboratories causes some difficulties in C. Read More

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Enzyme activities of Borrelia burgdorferi sensu lato.

Folia Microbiol (Praha) 2001 ;46(3):179-82

Department of Microbiology, Faculty of Science, Masaryk University, 602 00 Brno, Czechia.

Activities of 19 enzymes were tested by the API ZYM system in 13 strains of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto, B. afzelii, B. Read More

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Extracellular enzymatic activity in 11 Cryptococcus species.

Mycopathologia 2001 ;150(1):1-4

Hospital Universitario Puerta del Mar, Cádiz, Spain.

The extracellular enzymatic activity of 36 strains of yeast belonging to 11 species of the genus Cryptococcus, has been investigated, using the API-ZYM (BioMérieux, France) commercial system, with the objective of determining the differences in the enzymatic profiles of the various species. The strains studied were: 9 of C. neoformans, 7 of C. Read More

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October 2001

Extracellular enzymatic activity of Malassezia spp. isolates.

Mycopathologia 2001 ;149(3):131-5

Dipartimento di Patologia Animale, Profilassi ed Igiene degli Alimenti, Universitá di Pisa, Viale delle Piagge, 2, I 56100 Pisa, Italy.

Extracellular enzymatic activity of different species of Malassezia spp was evaluated. Thirty-three isolates of animal origin (dogs and cats) and stock culture samples were studied. Twenty isolates of M. Read More

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Legionella pneumophila major acid phosphatase and its role in intracellular infection.

Infect Immun 2001 Jan;69(1):177-85

Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA.

Legionella pneumophila is an intracellular pathogen of protozoa and alveolar macrophages. This bacterium contains a gene (pilD) that is involved in both type IV pilus biogenesis and type II protein secretion. We previously demonstrated that the PilD prepilin peptidase is crucial for intracellular infection by L. Read More

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January 2001

A study of the enzymatic profile of soil isolates of Nocardia asteroides.

Mycopathologia 1998-1999;143(3):151-4

Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.

In this study, using the API-ZYM system, we have reported the enzyme profile of 42 soil strains and 2 clinical strains of Nocardia asteroides isolated locally. Of the 19 enzymes tested, only 7 were demonstrable in over 90% of the soil isolates. These included alkaline phosphatase, esterase lipase, leucine arylamidase, acid phosphatase, phosphohydrolase, alpha-glucosidase and beta-glucosidase. Read More

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