85,596 results match your criteria phosphatase activity


One-component nano-metal-organic frameworks with superior multienzyme-mimic activities for 1,4-dihydropyridine metabolism.

J Colloid Interface Sci 2021 Jul 23;605:214-222. Epub 2021 Jul 23.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University Chongqing 400715, PR China. Electronic address:

Although a number of nanozymes have been developed, it is still difficult to develop single-component nanozyme with overall high multienzyme-like activities. In this study, the nanosized metal-organic frameworks (nano-MOFs) FePCN (PCN stands for porous coordination network) was synthesized by integrating zirconium and iron ions with different catalytic property on single-component MOFs and exhibited superior intrinsic multienzyme-like activities, namely oxidase-, peroxidase- and phosphatase-mimicking activity. The catalytic active sites of oxidase- and peroxidase-, and phosphatase-like activity of FePCN were Fe-centers and Zr-O clusters, respectively. Read More

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Short carboxyl terminal parathyroid hormone peptides modulate human parathyroid hormone signaling in mouse osteoblasts.

Biochem Biophys Res Commun 2021 Jul 28;572:15-19. Epub 2021 Jul 28.

Division of Nephrology and Hypertension, Department of Medicine, Mayo Clinic Rochester, 200 First Street Southwest, Rochester, MN, 55905, USA; Department of Biochemistry and Molecular Biology, Mayo Clinic Rochester, 200 First Street Southwest, Rochester, MN, 55905, USA. Electronic address:

Background: Novel human parathyroid hormone (hPTH) peptides of unknown biological activity have recently been identified in the serum of subjects with normal renal function, chronic renal failure, and end-stage renal disease through the application of liquid chromatography-high resolution mass spectrometry.

Purpose: of experiments: To determine the bioactivity of these peptides, we synthesized hPTH28-84, hPTH38-84, and hPTH45-84 peptides by solid phase peptide synthesis and tested their bioactivity in MC3T3-E1 mouse osteoblasts, either individually or together with the native hormone, hPTH1-84, by assessing the accumulation of 3´,5´-cyclic adenosine monophosphate (cAMP) and the induction of alkaline phosphatase activity.

Results: Increasing doses of hPTH1-84 (1-100 nM) increased the accumulation of cAMP and alkaline phosphatase activity in osteoblasts. Read More

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Sphingomyelin synthase related protein is a mammalian phosphatidylethanolamine phospholipase C.

Biochim Biophys Acta Mol Cell Biol Lipids 2021 Jul 28:159017. Epub 2021 Jul 28.

Department of Cell Biology, SUNY Downstate Health Sciences University, Brooklyn, New York, USA, 11203; Molecular and Cellular Cardiology Program, VA, New York Harbor Healthcare System, Brooklyn, NY 11209. Electronic address:

Sphingomyelin synthase related protein (SMSr) has no SM synthase activity but has ceramide phosphorylethanolamine (CPE) synthase activity in vitro. Although SMSr is ubiquitously expressed in all tested tissues, the CPE levels in most mammalian tissues or cells are extremely low or undetectable. Therefore, SMSr seems not to be a functional CPE synthase in vivo and its real biological function needs to be elucidated. Read More

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ANTI-OSTEOBLASTOGENIC, PRO-INFLAMMATORY AND PRO-ANGIOGENIC EFFECT OF EXTRACELLULAR VESICLES ISOLATED FROM THE HUMAN OSTEOSARCOMA CELL LINE MNNG/HOS.

Bone 2021 Jul 27:116130. Epub 2021 Jul 27.

Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy. Electronic address:

Extracellular Vesicles (EVs) are becoming increasingly recognized as integral signaling vehicles in several types of cancers, including bone malignancies. However, the specific mechanisms by which EVs influence osteosarcoma progression have not been fully determined. We evaluated the effects of EVs derived from the human osteosarcoma cell line MNNG/HOS (MNNG/HOS-EVs) on bone resident cells. Read More

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Expression of the developmental important candidate genes in oocytes, embryos, embryonic stem cells, cumulus cells, and fibroblast cells of buffalo (Bubalus bubalis).

Gene Expr Patterns 2021 Jul 27:119200. Epub 2021 Jul 27.

Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, India. Electronic address:

The present study was undertaken to study the expression of the developmental important gene transcripts in immature oocytes, mature oocytes, different stages of IVF produced embryos, embryonic stem (ES), cumulus (BCC), fetal fibroblast (BFF), newborn fibroblast (NBF) and adult fibroblast (BAF) cells of buffalo by semi-quantitative RT-PCR. The expression of GLUT1, HSP70.1, POL A Polymerase, GDF9, BMP15, and SURVIVIN transcripts was found in immature oocytes, mature oocytes, 2-cell, 4-cell, 8-16 cell, morula, and the blastocyst. Read More

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Supplementation of cumin seed powder prevents oxidative stress, hyperlipidemia and non-alcoholic fatty liver in high fat diet fed rats.

Biomed Pharmacother 2021 Jul 10;141:111908. Epub 2021 Jul 10.

Department of Pharmaceutical Sciences, North South University Bangladesh, Bangladesh. Electronic address:

The present investigation was an attempt to evaluate the hypoglycemic, lipid-lowering, antioxidant and hepatoprotective effects of cumin (Cuminum cyminum family: Apiaceae) supplementation in high fat (HF) diet fed rats. Male Wistar rats were divided into four groups, such as control, control+ cumin, HF and HF+ cumin. Oral glucose tolerance test, plasma lipids, oxidative stress parameters, antioxidant enzymes activities, and liver dysfunction marker enzyme activities were evaluated. Read More

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Decarboxylated osteocalcin, a possible drug for type 2 diabetes, triggers glucose uptake in MG63 cells.

World J Diabetes 2021 Jul;12(7):1102-1115

Department of Endocrinology, The Fourth Affiliated Hospital of China Medical University, Shenyang 110032, Liaoning Province, China.

Background: Uncarboxylated osteocalcin (GluOC) has been reported to improve glucose metabolism, prevent type 2 diabetes, and decrease the severity of obesity in mice with type 2 diabetes. GluOC can increase glucose uptake in a variety of cells. Glucose metabolism is the main source of energy for osteoblast proliferation and differentiation. Read More

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Soluble mannose receptor induces proinflammatory macrophage activation and metaflammation.

Proc Natl Acad Sci U S A 2021 Aug;118(31)

Cellular Immunology, Life and Medical Sciences Institute, University of Bonn, 53115 Bonn, Germany;

Proinflammatory activation of macrophages in metabolic tissues is critically important in the induction of obesity-induced metaflammation. Here, we demonstrate that the soluble mannose receptor (sMR) plays a direct functional role in both macrophage activation and metaflammation. We show that sMR binds CD45 on macrophages and inhibits its phosphatase activity, leading to an Src/Akt/NF-κB-mediated cellular reprogramming toward an inflammatory phenotype both in vitro and in vivo. Read More

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Serial administration of rhBMP-2 and alendronate enhances the differentiation of osteoblasts.

Int J Rheum Dis 2021 Jul 29. Epub 2021 Jul 29.

Division of Rheumatology, Department of Internal Medicine, Keimyung University School of Medicine, Daegu, Korea.

Aim: The incorporation of growth factors is an effective strategy to accelerate bone induction. Bone morphogenetic protein-2 (BMP-2) promotes osteoblast differentiation and induces bone formation. Alendronate (ALN) is an osteoclast deactivation drug. Read More

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Effects of antimony (III/V) on microbial activities and bacterial community structure in soil.

Sci Total Environ 2021 May 27;789:148073. Epub 2021 May 27.

State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China.

Antimony (Sb) primarily exists in trivalent (III) and pentavalent (V) speciation in the soil environment and poses a potential threat to ecological soil function as a toxic metalloid element. To evaluate the ecological effect of Sb in soil, the effects of different concentrations of Sb(III) and Sb(V) on microbial biomass carbon (MBC), soil basal respiration (SBR), potential nitrification rate (PNR), five enzyme activities and bacterial community structure were investigated using biochemical methods and high-throughput sequencing technology during the 1st and 8th weeks of exposure. The results of these experiments indicated that MBC and SBR were influenced, and PNR, FDA hydrolysis activity and urease activity were significantly inhibited by Sb(III) and Sb(V), while the activities of dehydrogenase, acid phosphatase and β-glucosidase had no clear effects on the amounts of Sb(III) or Sb(V). Read More

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Atmospheric nitrogen enrichment changes nutrient stoichiometry and reduces fungal N supply to peatland ericoid mycorrhizal shrubs.

Sci Total Environ 2021 Jun 28;794:148737. Epub 2021 Jun 28.

Natural Resources Institute Finland (Luke), Finland.

Peatlands store one third of global soil carbon (C) and up to 15% of global soil nitrogen (N) but often have low plant nutrient availability owing to slow organic matter decomposition under acidic and waterlogged conditions. In rainwater-fed ombrotrophic peatlands, elevated atmospheric N deposition has increased N availability with potential consequences to ecosystem nutrient cycling. Here, we studied how 14 years of continuous N addition with either nitrate or ammonium had affected ericoid mycorrhizal (ERM) shrubs at Whim Bog, Scotland. Read More

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Osteogenic and Angiogenic Synergy of Human Adipose Stem Cells and Human Umbilical Vein Endothelial Cells Cocultured in a Modified Perfusion Bioreactor.

Organogenesis 2021 Jul 29:1-16. Epub 2021 Jul 29.

Department of Biomedical Engineering, Research Center for New Technologies in Life Science Engineering, University of Tehran, Tehran, Iran.

Synergistic promotion of angiogenesis and osteogenesis in bone tissue-engineered constructs remains a crucial clinical challenge, which might be overcome by simultaneous employment of superior techniques including coculture systems, differentiation-stimulated factors, combinatorial scaffolds and bioreactors.Current study investigated the effect of flow perfusion along with coculture of human adipose stem cells (hASCs) and human umbilical vein endothelial cells (HUVECs) on osteogenic and angiogenic differentiation.Pre-treated hASCs with 1,25-dihydroxyvitamin D were seeded onto poly(lactic-co-glycolic acid)/β-tricalcium phosphate/polycaprolactone (PLGA/β-TCP/PCL) scaffold with/without HUVECs, and cultured for 14 days within a flask or modified perfusion bioreactor. Read More

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Gallium (Ga)-strontium (Sr) layered double hydroxide composite coating on titanium substrates for enhanced osteogenic and antibacterial abilities.

J Biomed Mater Res A 2021 Jul 29. Epub 2021 Jul 29.

Laboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Bacterial infection and poor osteogenic capacity can result in the loosing or failure of titanium (Ti)-based implants in the clinic. Therefore, it is urgent to design an effective approach to enhance the osteogenic property and restrict bacterial activity. In this study, a layered double hydroxide (LDH) composed of Ga and Sr ions on Ti substrates by a hydrothermal method, then calcined in 250°C and denoted as LDH250. Read More

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Features of Bioaccumulation and Toxic Effects of Copper (II) Oxide Nanoparticles Under Repeated Oral Exposure in Rats.

Pharm Nanotechnol 2021 Jul 28. Epub 2021 Jul 28.

Federal Scientific Center for Medical and Preventive Health Risk Management Technologies Perm, Russian Federation.

Background: Currently, the range of copper (II) oxide nanoparticles' (CuO NPs) applications is expanding and the global production of CuO NPs is increasing. In this regard, the risk of exposure of the population to this nanomaterial increases.

Objective: The aim of the study is to investigate the patterns of bioaccumulation and toxic effects of CuO NPs after multiple oral exposures. Read More

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Diabetes Impaired Ischemia-Induced PDGF (Platelet-Derived Growth Factor) Signaling Actions and Vessel Formation Through the Activation of Scr Homology 2-Containing Phosphatase-1.

Arterioscler Thromb Vasc Biol 2021 Jul 29:ATVBAHA121316638. Epub 2021 Jul 29.

Research Center of the Centre Hospitalier Universitaire de Sherbrooke, Université de Sherbrooke, Québec, Canada. (C.M., T.B., J.L., E.B., S.R., V.B., M.P., A.G., F.L., P.G.).

Objective: Critical limb ischemia is a major complication of diabetes characterized by insufficient collateral vessel development and proper growth factor signaling unresponsiveness. Although mainly deactivated by hypoxia, phosphatases are important players in the deregulation of proangiogenetic pathways. Previously, SHP-1 (Scr homology 2-containing phosphatase-1) was found to be associated with the downregulation of growth factor actions in the diabetic muscle. Read More

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Sulforaphane alleviates hepatic ischemia-reperfusion injury through promoting the activation of Nrf-2/HO-1 signaling.

Transpl Immunol 2021 Jul 25:101439. Epub 2021 Jul 25.

General Practice Center, and University of Electronic Science and Technology, Sichuan Academy of Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China. Electronic address:

Background: Sulforaphane (SFN)displays both anti-oxidative stress and anti-inflammatory activity. Given that inflammation and oxidative stress play important roles in hepatic ischemia-reperfusion injury (HI/RI), we examined the protective effect and potential mechanism of SFN on HI/RI.

Methods: The maneuver of Pringle's was used to establish the mode of HI/RI and 60 SD rats were randomly divided into Sham, HI/RI, SFN and ML385 Groups. Read More

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On the PHLPPside: Emerging roles of PHLPP phosphatases in the heart.

Cell Signal 2021 Jul 25:110097. Epub 2021 Jul 25.

Department of Pharmacology, University of California, San Diego, La Jolla, CA 92039, USA; Cardiovascular Molecular Signaling, Huntington Medical Research Institutes, Pasadena, CA 91105, USA. Electronic address:

PH domain leucine-rich repeat protein phosphatase (PHLPP) is a family of enzymes made up of two isoforms (PHLPP1 and PHLPP2), whose actions modulate intracellular activity via the dephosphorylation of specific serine/threonine (Ser/Thr) residues on proteins such as Akt. Recent data generated in our lab, supported by findings from others, implicates the divergent roles of PHLPP1 and PHLPP2 in maintaining cellular homeostasis since dysregulation of these enzymes has been linked to various pathological states including cardiovascular disease, diabetes, ischemia/reperfusion injury, musculoskeletal disease, and cancer. Therefore, development of therapies to modulate specific isoforms of PHLPP could prove to be therapeutically beneficial in several diseases especially those targeting the cardiovascular system. Read More

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Nutrient deficiency and an algicidal bacterium improved the lipid profiles of a novel promising oleaginous dinoflagellate, for biodiesel production.

Appl Environ Microbiol 2021 Jul 28:AEM0115921. Epub 2021 Jul 28.

State Key Laboratory of Cellular Stress Biology, and School of Life Sciences, Xiamen University, Xiamen, Fujian, 361102, P. R. China.

The lipid production potential of 8 microalgae species was investigated. Among these eight species, the best strain was a dominant bloom-causing dinoflagellate, ; this species had a lipid content of 49.32±1. Read More

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Suppression of JAK-STAT signaling by Epstein-Barr virus tegument protein BGLF2 through recruitment of SHP1 phosphatase and promotion of STAT2 degradation.

J Virol 2021 Jul 28:JVI0102721. Epub 2021 Jul 28.

School of Biomedical Sciences, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong.

Some lytic proteins encoded by Epstein-Barr virus (EBV) suppress host interferon (IFN) signaling to facilitate viral replication. In this study we sought to identify and characterize EBV proteins antagonizing IFN signaling. The induction of IFN-stimulated genes (ISGs) by IFN-β was effectively suppressed by EBV. Read More

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Cooperative Kinetics of the Glucan Phosphatase Starch Excess4.

Biochemistry 2021 Jul 28. Epub 2021 Jul 28.

Department of Chemistry, Skidmore College, Saratoga Springs, New York 12866, United States.

Glucan phosphatases are members of a functionally diverse family of dual-specificity phosphatase (DSP) enzymes. The plant glucan phosphatase Starch Excess4 (SEX4) binds and dephosphorylates glucans, contributing to processive starch degradation in the chloroplast at night. Little is known about the complex kinetics of SEX4 when acting on its complex physiologically relevant glucan substrate. Read More

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Senescent RAW264.7 cells exhibit increased production of nitric oxide and release inducible nitric oxide synthase in exosomes.

Mol Med Rep 2021 Sep 28;24(3). Epub 2021 Jul 28.

Department of Oral and Maxillofacial Surgery, Hyogo College of Medicine, Nishinomiya, Hyogo 663‑8501, Japan.

Aging cells not only cease growing, but also secrete various proteins such as inflammatory cytokines. This secretory phenomenon is known as the senescence‑associated secretory phenotype (SASP). The aim of the present study was to elucidate the effects of senescence on the differentiation of osteoclast precursors (OCPs) and corresponding SASP. Read More

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September 2021

In vitro osteogenic induction of human adipose stem cells co-treated with betaine/osteogenesis differentiation medium.

Mol Biol Res Commun 2021 Jun;10(2):93-103

Department of Cellular and Molecular Biology, School of Biology and Institute of Biological Sciences, Damghan University, Damghan, Iran.

Human adipose-derived stem cells (hADSCs) are widely used in regenerative medicine and affected by many biochemical and biophysical stimuli in vivo. Betaine has been reported to be a type of osteogenic stimulating biochemical factor. This study aimed to investigate the effects of betaine; on osteogenic differentiation of cultured hADSCs in osteogenesis differentiation medium. Read More

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Utilization of soil residual phosphorus and internal reuse of phosphorus by crops.

PeerJ 2021 15;9:e11704. Epub 2021 Jul 15.

State Key Laboratory of Grassland Agro-ecosystems; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, P. R. China.

Phosphorus (P) participates in various assimilatory and metabolic processes in plants. Agricultural systems are facing P deficiency in many areas worldwide, while global P demand is increasing. Pioneering efforts have made us better understand the more complete use of residual P in soils and the link connecting plant P resorption to soil P deficiency, which will help to address the challenging issue of P deficiency. Read More

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Effect of physical activity and BMI SDS on bone metabolism in children and adolescents.

Bone 2021 Jul 24:116131. Epub 2021 Jul 24.

Department of Pediatric Surgery, University Hospital Leipzig, Leipzig, Germany. Electronic address:

Objective: Children with obesity are known to have reduced bone density and are at a higher risk for fractures. This may be caused by decreased physical activity or a metabolic phenomenon. In this study, we evaluated associations of physical activity with bone metabolism in children and adolescents with and without obesity. Read More

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Protein phosphatase 6 promotes neurite outgrowth by promoting mTORC2 activity in N2a cells.

J Biochem 2021 Jul 26. Epub 2021 Jul 26.

Laboratory of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515, Japan.

Understanding the molecular mechanism of neuronal differentiation is important to overcome the incurable diseases caused by nervous system damage. Neurite outgrowth is prerequisite for neuronal differentiation and regeneration, and cAMP response element-binding protein (CREB) is one of the major transcriptional factors positively regulating this process. Neuronal differentiation stimuli activate mammalian target of rapamycin (mTOR) complex 2 (mTORC2)/Akt signaling to phosphorylate CREB, however, the precise molecular mechanism of this event has not been fully understood. Read More

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A new layer of phosphoinositide-mediated allosteric regulation uncovered for SHIP2.

FASEB J 2021 08;35(8):e21815

Structural and Chemical Biology, Centro de Investigaciones Biológicas Margarita Salas, Spanish National Research Council (CSIC), Madrid, Spain.

The Src homology 2 containing inositol 5-phosphatase 2 (SHIP2) is a large multidomain enzyme that catalyzes the dephosphorylation of the phospholipid phosphatidylinositol 3,4,5-triphosphate (PI(3,4,5)P ) to form PI(3,4)P . PI(3,4,5)P is a key lipid second messenger controlling the recruitment of signaling proteins to the plasma membrane, thereby regulating a plethora of cellular events, including proliferation, growth, apoptosis, and cytoskeletal rearrangements. SHIP2, alongside PI3K and PTEN, regulates PI(3,4,5)P levels at the plasma membrane and has been heavily implicated in serious diseases such as cancer and type 2 diabetes; however, many aspects of its regulation mechanism remain elusive. Read More

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The intermediate-conductance calcium-activated potassium channel KCa3.1 contributes to alkalinization-induced vascular calcification in vitro.

J Clin Lab Anal 2021 Jul 27:e23854. Epub 2021 Jul 27.

Hebei Clinical Research Center for Chronic Kidney Disease, Hebei Key Laboratory of Vascular Calcification in Kidney Disease, Departments of Nephrology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.

Objective: In order to find new strategies for the prevention of vascular calcification in uremic individuals especially treated by dialysis and develop novel therapeutic targets in vascular calcification, we explore the role of KCa3.1 in alkalinization-induced VSMCs calcification in vitro.

Method: Rat VSMCs calcification model was established by beta-glycerophosphate (β-GP, 10 mM) induction. Read More

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A model based on clinico-biochemical characteristics and deep learning features from MR images for assessing necroinflammatory activity in chronic hepatitis B.

J Viral Hepat 2021 Jul 27. Epub 2021 Jul 27.

Department of Radiology, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510120, China.

Accurate liver necroinflammatory activity diagnosis could guide the clinical decision-making in chronic hepatitis B (CHB) patients.This study aimed to build a non-invasive diagnostic model for liver necroinflammatory activity by incorporating deep learning features and clinico-biochemical characteristics in CHB patients. A total of 239 CHB patients who underwent liver biopsy were recruited and randomly divided into a training cohort (n = 179) and an independent validation cohort (n = 60). Read More

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CLEC12B decreases melanoma proliferation by repressing STAT3.

J Invest Dermatol 2021 Jul 23. Epub 2021 Jul 23.

Université Nice Côte d'Azur, INSERM, U1065, Centre Méditerranéen de Médecine Moléculaire (C3M), team 12, Nice, France; Université Nice Côte d'Azur, CHU Nice, Department of Dermatology, Nice, France. Electronic address:

The potential role of CLEC12B, a gene predominantly expressed by skin melanocytes discovered through transcriptomic analysis, in melanoma is unknown. Here we show that CLEC12B expression is lower in melanoma and melanoma metastases than in melanocytes and benign melanocytic lesions, and that its decrease correlates with a poor prognosis. We further show that CLEC12B recruits SHP2 phosphatase through its ITIM domain, inactivates STAT1/3/5, increases p53/p21/p27 expression/activity and modulates melanoma cell proliferation. Read More

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Micro-RNA 133a-3p induces repolarization abnormalities in atrial myocardium and modulates ventricular electrophysiology affecting I and Ito currents.

Eur J Pharmacol 2021 Jul 24;908:174369. Epub 2021 Jul 24.

Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK; Heart Embryology and Anatomy Research Team, Department of Anatomy, Jagiellonian University Medical College, Cracow, Poland.

Mir-133a-3p is the most abundant myocardial microRNA. The impact of mir-133a-3p on cardiac electrophysiology is poorly explored. In this study, we investigated the effects of mir-133a-3p on the main ionic currents critical for action potential (AP) generation and electrical activity of the heart. Read More

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