Appl Environ Microbiol 2021 Mar 19. Epub 2021 Mar 19.
Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Australia.
MalF has been shown to be required for virulence in the important avian pathogen To characterise the function of MalF, predicted to be part of putative ABC transporter, we compared metabolite profiles of a mutant with a transposon inserted in (MalF-deficient ST mutant 04-1; Δ) with those of wild type bacteria using GC/MS and LC/MS. Of those substrates likely to be transported by an ABC transport system, glycerol was detected at significantly lower abundance in the Δ mutant, when compared to the wild type. Stable isotope labelling using [U-C] glycerol and RT-qPCR analysis indicated that MalF was responsible for import of glycerol into and that, in the absence of MalF, the transcription of which encodes a second transporter, GtsA, was upregulated, potentially to increase import of glycerol-3-phosphate into the cell to compensate for the loss of MalF. Read More