2,886,467 results match your criteria peptides


Ultrafiltration strategy combined with nanoLC-MS/MS based proteomics for monitoring potential residual proteins in TCMIs.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 May 30;1178:122818. Epub 2021 May 30.

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address:

Traditional Chinese medicine injections (TCMIs) containing complex constituents frequently cause unpredictable adverse reactions. The residual heterologous proteins in TCMIs may be one kind of the sensitized constituents. However, few methods were developed to identify and monitor the residual proteins of TCMIs in industry. Read More

View Article and Full-Text PDF

Dissection of phospholipases A reveals multifaceted peptides targeting cancer cells, Leishmania and bacteria.

Bioorg Chem 2021 May 31;114:105041. Epub 2021 May 31.

Biomolecules Discovery Group, Universidad Regional Amazónica Ikiam, Km 7 Via Muyuna, Tena, Napo, Ecuador. Electronic address:

Cationic peptides bio-inspired by natural toxins have been recognized as an efficient strategy for the treatment of different health problems. Due to the specific interaction with substrates from biological membranes, snake venom phospholipases (PLAs) represent valuable scaffolds for the research and development of short peptides targeting parasites, bacteria, and cancer cells. Considering this, we evaluated the in vitro therapeutic potential of three biomimetic peptides (pCergo, pBmTxJ and pBmje) based on three different amino acid sequences from Asp49 PLAs. Read More

View Article and Full-Text PDF

Metabolic and conformational stabilization of a VEGF-mimetic beta-hairpin peptide by click-chemistry.

Eur J Med Chem 2021 May 26;222:113575. Epub 2021 May 26.

Istituto di Scienze e Tecnologie Chimiche "Giulio Natta", CNR, Via M. Bianco 9, 20131, Milano, Italy. Electronic address:

HPLW is a Vascular Endothelial Growth Factor (VEGF)-mimicking beta-hairpin peptide endowed of proangiogenic effect and showing valuable biomedical application in the proangiogenic therapy. However, the translational potential of HPLW is limited by its low metabolic stability, which would shorten the in vivo efficacy of the molecule. Here, we developed a peptide analog of HPLW, named HPLW2, that retains the structural and biological properties of the original peptide but features an impressive resistance to degradation by human serum proteases. Read More

View Article and Full-Text PDF

Deeply Mining a Universe of Peptides Encoded by Long Noncoding RNAs.

Mol Cell Proteomics 2021 Jun 12:100109. Epub 2021 Jun 12.

Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Many small open reading frames (smORFs) embedded in lncRNA transcripts have been shown to encode biologically functional polypeptides (smORFs-encoded polypeptides, SEPs) in different organisms. Despite significant advances in genomics, bioinformatics and proteomics that largely enabled the discovery of novel SEPs, their identification across different biological samples is still hampered by their poor predictability, diminutive size and low relative abundance. Here, we take advantage of NONCODE, a repository containing the most complete collection and annotation of lncRNA transcripts from different species, to build a novel database that attempts to maximize a collection of SEPs from human and mouse lncRNA transcripts. Read More

View Article and Full-Text PDF

Trimethylacetic anhydride-based derivatization facilitates quantification of histone marks at the MS1 level.

Mol Cell Proteomics 2021 Jun 12:100114. Epub 2021 Jun 12.

Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic; Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic. Electronic address:

Histone post-translational modifications (hPTMs) are epigenetic marks that strongly affect numerous processes, including cell cycling and protein interactions. They have been studied by both antibody- and mass spectrometry-based methods for years, but the analyses are still challenging, mainly due to the diversity of histones and their modifications arising from high contents of reactive amine groups in their amino acid sequences. Here, we introduce use of trimethylacetic anhydride (TMA) as a new reagent for efficient histone derivatization, which is a requirement for bottom-up proteomic hPTM analysis. Read More

View Article and Full-Text PDF

MHC Phosphopeptides: Promising targets for immunotherapy of cancer and other chronic diseases.

Mol Cell Proteomics 2021 Jun 12:100112. Epub 2021 Jun 12.

Department of Chemistry, University of Virginia, Charlottesville, VA, USA; Department of Pathology, University of Virginia, Charlottesville, VA, USA. Electronic address:

Major histocompatibility complex (MHC)-associated peptides have been considered as potential immunotherapeutic targets for many years. MHC class I phosphopeptides result from dysregulated cell signaling pathways that are common across cancers and both viral and bacterial infections. These antigens are recognized by central memory T-cells from healthy donors, indicating that they are considered antigenic by the immune system and that they are presented across different individuals and diseases. Read More

View Article and Full-Text PDF

Pitfalls in HLA ligandomics - How to catch a li(e)gand.

Mol Cell Proteomics 2021 Jun 12:100110. Epub 2021 Jun 12.

Immatics Biotechnologies GmbH, Paul-Ehrlich-Str. 15-17, 72076 Tübingen, Germany; Immatics US, Inc. 2201 W. Holcombe Blvd., Suite 205, Houston, Texas 77030, USA. Electronic address:

Knowledge about the peptide repertoire presented by human leukocyte antigens (HLA) holds the key to unlock target-specific cancer immunotherapies like adoptive cell therapies or bispecific T cell engaging receptors. Therefore, comprehensive and accurate characterization of HLA peptidomes by mass spectrometry (immunopeptidomics) across tissues and disease states is essential. With growing numbers of immunopeptidomics datasets and the scope of peptide identification strategies reaching beyond the canonical proteome, the likelihood for erroneous peptide identification as well as false annotation of HLA-independent peptides as HLA ligands is increasing. Read More

View Article and Full-Text PDF

Sensitive and quantitative detection of MHC-I displayed neoepitopes using a semi-automated workflow and TOMAHAQ mass spectrometry.

Mol Cell Proteomics 2021 Jun 12:100108. Epub 2021 Jun 12.

Genentech, South San Francisco, 94080, California, USA. Electronic address:

Advances in several key technologies, including MHC peptidomics, have helped fuel our understanding of basic immune regulatory mechanisms and the identification of T cell receptor targets for the development of immunotherapeutics. Isolating and accurately quantifying MHC-bound peptides from cells and tissues enables characterization of dynamic changes in the ligandome due to cellular perturbations. However, the current multi-step analytical process is challenging, and improvements in throughput and reproducibility would enable rapid characterization of multiple conditions in parallel. Read More

View Article and Full-Text PDF

New bioactive peptides from the venom gland of social hornet Vespa velutina.

Toxicon 2021 Jun 12. Epub 2021 Jun 12.

CAS Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, 650000, China. Electronic address:

Bacterial resistance to drugs is a global problem requiring the urgent development of new antibiotics. Antimicrobial peptides (AMPs) are excellent candidates for the design of novel antibiotics to combat microbial resistance. In this research, we identified four new peptides (U-VVTX-Vp1a, U-VVTX-Vp1b, U-VVTX-Vp2a, and U-VVTX-Vp2b, respectively) from the venom of Vespa velutina, and tested their antimicrobial, antioxidant, and hemolytic effects. Read More

View Article and Full-Text PDF

Isabella L. Karle: A Crystallography Pioneer.

Authors:
Tamar Schlick

DNA Cell Biol 2021 Jun 14. Epub 2021 Jun 14.

Department of Chemistry and Courant Institute of Mathematical Sciences, New York University, New York, New York, USA.

The life and work of crystallography pioneer Isabella L. Karle is recounted (1921-2017), as researched from the literature and personal stories of colleagues and family. Her story includes her family background, education at the University of Michigan, research on the Manhattan Project, and 63 productive years at the Naval Research Laboratory. Read More

View Article and Full-Text PDF

Asparaginyl Ligase-Catalyzed One-Step Cell Surface Modification of Red Blood Cells.

ACS Chem Biol 2021 Jun 15. Epub 2021 Jun 15.

Program in Cellular and Molecular Medicine, Boston Children's Hospital, Harvard Medical School, Boston, Massachussets 02115, United States.

Red blood cells (RBCs) can serve as vascular carriers for drugs, proteins, peptides, and nanoparticles. Human RBCs remain in the circulation for ∼120 days, are biocompatible, and are immunologically largely inert. RBCs are cleared by the reticuloendothelial system and can induce immune tolerance to foreign components attached to the RBC surface. Read More

View Article and Full-Text PDF

Upregulation of antigen presentation function and inflammation in chondrocytes by induction of proteoglycan aggrecan peptides (P16-31 and P263-280).

Clin Exp Rheumatol 2021 Jun 8. Epub 2021 Jun 8.

Immunology Division, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, and Skeletal Disorders Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.

Objectives: A central hallmark of osteoarthritis (OA) is cartilage destruction. Chondrocytes not only control cartilage metabolism, but are capable of immunogenic responses. The role of chondrocytes in the pathogenesis of OA is still unclear. Read More

View Article and Full-Text PDF

Transient Permeation Enhancer® (TPE®) technology for oral delivery of octreotide: a technological evaluation.

Expert Opin Drug Deliv 2021 Jun 15. Epub 2021 Jun 15.

School of Pharmacy and Biomolecular Sciences, Royal College of Surgeons in Ireland, St. Stephen's Green, Dublin 2, Ireland.

Introduction: : The FDA approval of oral semaglutide for type 2 diabetes (2019) and oral octreotide for acromegaly (2020) is evidence that selected niche peptides can be administered orally if formulated with selected intestinal permeation enhancers.

Areas Covered: We evaluated the oral octreotide formulation, MYCAPSSA® (Chiasma Pharmaceuticals, Needham, MA, USA). An outline of the current standard of care in acromegaly and the benefits of oral octreotide versus depot injections is provided. Read More

View Article and Full-Text PDF

Antibody Levels to Plasmodium falciparum Erythrocyte Membrane Protein 1-DBLγ11 and DBLδ-1 Predict Reduction in Parasite Density.

mSystems 2021 Jun 15:e0034721. Epub 2021 Jun 15.

Molecular and Pathogenesis Biomarkers Section, Laboratory of Malaria Immunology and Vaccinology (LMIV), National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Bethesda, Maryland, USA.

Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is a variant surface antigen family expressed on infected red blood cells that plays a role in immune evasion and mediates adhesion to vascular endothelium. PfEMP1s are potential targets of protective antibodies as suggested by previous seroepidemiology studies. Here, we used previously reported proteomic analyses of PfEMP1s of clinical parasite isolates collected from Malian children to identify targets of immunity. Read More

View Article and Full-Text PDF

Glycosylation of a Nonfibrillizing Appendage Alters the Self-Assembly Pathway of a Synthetic β-Sheet Fibrillizing Peptide.

J Phys Chem B 2021 Jun 15. Epub 2021 Jun 15.

J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611, United States.

Owing to their biocompatibility and biodegradability, short synthetic peptides that self-assemble into elongated β-sheet fibers (i.e., peptide nanofibers) are widely used to create biomaterials for diverse medical and biotechnology applications. Read More

View Article and Full-Text PDF

Injectable and pH-Sensitive Hyaluronic Acid-Based Hydrogels with On-Demand Release of Antimicrobial Peptides for Infected Wound Healing.

Biomacromolecules 2021 Jun 15. Epub 2021 Jun 15.

Hubei Engineering Research Center for Biomaterials and Medical Protective Materials, School of Chemistry and Chemical Engineering, HUST, Wuhan 430074, China.

Antibiotics' abuse in bacteria-infected wounds has threatened patients' lives and burdened medical systems. Hence, antibiotic-free hydrogel-based biomaterials, which exhibit biostability, on-demand release of antibacterial agents, and long-lasting antimicrobial activity, are highly desired for the treatment of chronic bacteria-infected wounds. Herein, we developed a hyaluronic acid ()-based composite hydrogel, with an antimicrobial peptide [, KK(SLKL)KK] as a cross-linking agent through Schiff's base formation, which exhibited an acidity-triggered release of (pathological environment in bacteria-infected wounds, pH ∼ 5. Read More

View Article and Full-Text PDF

Tuning the Diameter, Stability, and Membrane Affinity of Peptide Pores by DNA-Programmed Self-Assembly.

ACS Nano 2021 Jun 15. Epub 2021 Jun 15.

Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland.

Protein pores recently enabled a breakthrough in bioanalytics by making it possible to sequence individual DNA and RNA strands during their translocation through the lumen of the pore. Despite this success and the overall promise of nanopore-based single-molecule analytics, protein pores have not yet reached their full potential for the analysis and characterization of globular biomolecules such as natively folded proteins. One reason is that the diameters of available protein pores are too small for accommodating the translocation of most folded globular proteins through their lumen. Read More

View Article and Full-Text PDF

Sequence-selective recognition of cationic amphipathic tripeptides with similar structures in aqueous solutions by cucurbit[7]uril.

Phys Chem Chem Phys 2021 Jun 15. Epub 2021 Jun 15.

Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photoelectronic/Electro-photonic Conversion Materials, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081, China.

Sequence-selective recognition of cationic amphipathic peptides by synthetic receptors is significant to biological applications, but it is still a great challenging task. Here we first study the binding characteristics of receptor cucurbit[7]uril (CB[7]) to the smallest aromatic tripeptides X1GG (X1 = tryptophan (W), phenylalanine (F), and tyrosine (Y)) and basic tripeptides X2GG (X2 = arginine (R), lysine (K), and histidine (H)) by molecular dynamics simulations. The study indicates that the sidechains of aromatic X1 residues can be encapsulated into the CB[7] cavity, while the sidechains of basic X2 residues prefer to locate at the CB[7] portal. Read More

View Article and Full-Text PDF

Recent advances (2019-2021) of capillary electrophoresis-mass spectrometry for multilevel proteomics.

Mass Spectrom Rev 2021 Jun 15. Epub 2021 Jun 15.

Department of Chemistry, Michigan State University, East Lansing, Michigan, USA.

Multilevel proteomics aims to delineate proteins at the peptide (bottom-up proteomics), proteoform (top-down proteomics), and protein complex (native proteomics) levels. Capillary electrophoresis-mass spectrometry (CE-MS) can achieve highly efficient separation and highly sensitive detection of complex mixtures of peptides, proteoforms, and even protein complexes because of its substantial technical progress. CE-MS has become a valuable alternative to the routinely used liquid chromatography-mass spectrometry for multilevel proteomics. Read More

View Article and Full-Text PDF

The membrane-targeting mechanism of host defense peptides inspiring the design of polypeptide-conjugated gold nanoparticles exhibiting effective antibacterial activity against methicillin-resistant .

J Mater Chem B 2021 Jun 15. Epub 2021 Jun 15.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. and Key Laboratory for Ultrafine Materials of Ministry of Education, Frontiers Science Center for Materiobiology and Dynamic Chemistry, Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, China.

Multidrug-resistant bacterial infections are a grand challenge to global medical and health systems. Therefore, it is urgent to develop versatile antibacterial strategies that can combat bacterial resistance without displaying toxicity. Here, we synthesize antibacterial polypeptide-conjugated gold nanoparticles that exhibit potent antibacterial activities against clinically isolated multiple drug resistance Gram-positive bacteria, such as methicillin-resistant Staphylococcus aureus, and excellent in vitro and in vivo biocompatibility. Read More

View Article and Full-Text PDF

Peptide profiling in cow urine reveals molecular signature of physiology-driven pathways and in-silico predicted bioactive properties.

Sci Rep 2021 Jun 14;11(1):12427. Epub 2021 Jun 14.

ICAR-National Dairy Research Institute, Cell Biology and Proteomics Lab, Animal Biotechnology Center (ABTC), Karnal, Haryana, 132001, India.

Peptidomics allows the identification of peptides that are derived from proteins. Urinary peptidomics has revolutionized the field of diagnostics as the samples represent complete systemic changes happening in the body. Moreover, it can be collected in a non-invasive manner. Read More

View Article and Full-Text PDF

Cyclotide host-defense tailored for species and environments in violets from the Canary Islands.

Sci Rep 2021 Jun 14;11(1):12452. Epub 2021 Jun 14.

Department of Pharmaceutical Biosciences, Pharmacognosy, Uppsala University, Biomedical Centre (BMC) 574, 75123, Uppsala, Sweden.

Cyclotides are cyclic peptides produced by plants. Due to their insecticidal properties, they are thought to be involved in host defense. Violets produce complex mixtures of cyclotides, that are characteristic for each species and variable in different environments. Read More

View Article and Full-Text PDF

Kim-1 Targeted Extracellular Vesicles: A New Therapeutic Platform for RNAi to Treat AKI.

J Am Soc Nephrol 2021 Jun 14. Epub 2021 Jun 14.

B Liu, Institute of Nephrology, Southeast University Zhongda Hospital, Nanjing, China

Acute kidney injury (AKI) is a significant public health problem with high morbidity and mortality. Unfortunately, no definitive treatment is currently available for AKI. RNA interference (RNAi) provides a new and potent method for gene therapy to tackle this dilemma. Read More

View Article and Full-Text PDF

Landscape and selection of vaccine epitopes in SARS-CoV-2.

Genome Med 2021 06 14;13(1):101. Epub 2021 Jun 14.

Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, CB# 7295, Chapel Hill, NC, 27599-7295, USA.

Background: Early in the pandemic, we designed a SARS-CoV-2 peptide vaccine containing epitope regions optimized for concurrent B cell, CD4 T cell, and CD8 T cell stimulation. The rationale for this design was to drive both humoral and cellular immunity with high specificity while avoiding undesired effects such as antibody-dependent enhancement (ADE).

Methods: We explored the set of computationally predicted SARS-CoV-2 HLA-I and HLA-II ligands, examining protein source, concurrent human/murine coverage, and population coverage. Read More

View Article and Full-Text PDF

Survey of in-silico prediction of anticancer peptides.

Authors:
Nan Ye

Curr Top Med Chem 2021 Jun 11. Epub 2021 Jun 11.

School of Finance and Economics, Xinyang Agriculture and Forestry University, Xinyang 464000, China.

Cancer is one of the major causes of death among human beings. Traditional treatments of cancer kill cancerous cells and negatively affect normal cells. The side effects and high medical costs prevent effective treatment. Read More

View Article and Full-Text PDF

Targeting Bacterial Membrane Proteins to Explore the Beneficial Effects of Natural Products: New Antibiotics against Drug Resistance.

Curr Med Chem 2021 Jun 14. Epub 2021 Jun 14.

State Key Laboratory of Medical Molecular Biology, Department of Microbiology and Parasitology, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.

Antibiotic resistance is currently a world health crisis that urges the development of new antibacterial substances. To this end, natural products, including flavonoids, alkaloids, terpenoids, steroids, peptides and organic acids that play a vital role in the development of medicines and thus constitute a rich source in clinical practices, provide an important source of drugs directly or for the screen of lead compounds for new antibiotic development. Because membrane proteins, which comprise more than 60% of the current clinical drug targets, play crucial roles in signal transduction, transport, bacterial pathogenicity and drug resistance, as well as immunogenicity, it is our aim to summarize those natural products with different structures that target bacterial membrane proteins, such as efflux pumps and enzymes, to provide an overview for the development of new antibiotics to deal with antibiotic resistance. Read More

View Article and Full-Text PDF

A step-economic and one-pot access to chiral C-tetrasubstituted α-amino acid derivatives a bicyclic imidazole-catalyzed direct enantioselective -acylation.

Chem Sci 2020 Apr 24;11(18):4801-4807. Epub 2020 Apr 24.

Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs, School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Shanghai Jiao Tong University Shanghai 200240 China

C-Tetrasubstituted α-amino acids are ubiquitous and unique structural units in bioactive natural products and pharmaceutical compounds. The asymmetric synthesis of these molecules has attracted a lot of attention, but a more efficient method is still greatly desired. Here we describe the first sequential four-step acylation reaction for the efficient synthesis of chiral C-tetrasubstituted α-amino acid derivatives from simple -acylated amino acids an auto-tandem catalysis using a single nucleophilic catalyst. Read More

View Article and Full-Text PDF

Engineering bio-mimicking functional vesicles with multiple compartments for quantifying molecular transport.

Chem Sci 2020 Apr 6;11(18):4669-4679. Epub 2020 Apr 6.

Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST) Trivandrum 695019 Kerala India

Controlled design of giant unilamellar vesicles under defined conditions has vast applications in the field of membrane and synthetic biology. Here, we bio-engineer bacterial-membrane mimicking models of controlled size under defined salt conditions over a range of pH. A complex bacterial lipid extract is used for construction of physiologically relevant Gram-negative membrane mimicking vesicles whereas a ternary mixture of charged lipids (DOPG, cardiolipin and lysyl-PG) is used for building Gram-positive bacterial-membrane vesicles. Read More

View Article and Full-Text PDF

Peptidomimetics prepared by tail-to-side chain one component peptide stapling inhibit Alzheimer's amyloid-β fibrillogenesis.

Chem Sci 2020 Mar 27;11(16):4171-4179. Epub 2020 Mar 27.

Laboratory of Peptide and Amyloid Research, Department of Chemistry, Indian Institute of Technology Guwahati Assam-781039 India

Alzheimer's disease (AD) is the most common form of dementia affecting the elderly population worldwide. Despite enormous efforts and considerable advancement in research, no therapeutic agents have come to light to date. However, many peptide-based and small molecule inhibitors interact efficiently with the amyloid-β (Aβ) peptide and alter its aggregation pathway. Read More

View Article and Full-Text PDF

An ornithine-rich dodecapeptide with improved proteolytic stability selectively kills gram-negative food-borne pathogens and its action mode on Escherichia coli O157:H7.

Int J Food Microbiol 2021 Aug 8;352:109281. Epub 2021 Jun 8.

State Key Laboratory of Chemical Biology and Drug Discovery and Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.

Food-borne pathogenic bacteria are dispersed throughout the entire chain of the food industry. However, many food preservatives are limited by poor biocompatibility such as cumulative poisoning. The antimicrobial peptide is increasingly regarded as a promising preservative in food research due to its high bioactivity and low cytotoxicity. Read More

View Article and Full-Text PDF