62 results match your criteria pelota

mRNA Surveillance Complex PELOTA-HBS1 Regulates Phosphoinositide-Dependent Protein Kinase1 and Plant Growth.

Plant Physiol 2021 Apr 30. Epub 2021 Apr 30.

Joint Centre for Single Cell Biology, School of Agriculture and Biology, Shanghai Jiao Tong University, 200240 Shanghai, China.

The quality control system for messenger RNA (mRNA) is fundamental for cellular activities in eukaryotes. To elucidate the molecular mechanism of 3'-Phosphoinositide-Dependent Protein Kinase1 (PDK1), a master regulator that is essential throughout eukaryotic growth and development, we employed a forward genetic approach to screen for suppressors of the loss-of-function T-DNA insertion double mutant pdk1.1 pdk1. Read More

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Perturbed differentiation of murine embryonic stem cells upon Pelota deletion due to dysregulated FOXO1/β-catenin signaling.

FEBS J 2020 Nov 27. Epub 2020 Nov 27.

Institute of Human Genetics, University Medical Centre, Göttingen, Germany.

Differentiation of the embryonic stem cells (ESCs) is regulated by a variety of different signaling pathways. Genetic depletion of murine Pelota gene (Pelo) leads to early embryonic lethality. Here, we aimed at determining the embryonic stage and deciphering the dysregulated signaling pathways affected upon Pelo deletion. Read More

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November 2020

Technical benefit on apple fruit of controlled atmosphere influenced by 1-MCP at molecular levels.

Mol Genet Genomics 2020 Nov 22;295(6):1443-1457. Epub 2020 Jul 22.

EMBRAPA Uva e Vinho, R. Livramento 515, Bento Gonçalves, RS, 957000-000, Brazil.

The apple is a highly perishable fruit after harvesting and, therefore, several storage technologies have been studied to provide the consumer market with a quality product with a longer shelf life. However, little is known about the apple genome that is submitted to the storage, and even less with the application of ripening inhibitors. Due to these factors, this study sought to elucidate the transcriptional profile of apple cultivate Gala stored in a controlled atmosphere (AC) treated and not treated with 1-methyl cyclopropene (1-MCP). Read More

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November 2020

Post-Traumatic Clinodactyly of the Little Finger in Basque Hand-Pelota Players.

Rev Esp Cir Ortop Traumatol 2020 May - Jun;64(3):160-166. Epub 2020 Feb 24.

Servicio de Cirugía Ortopédica y Traumatología, Clínica Universidad de Navarra, Pamplona, Navarra, España.

Objective: The aim of this study is to measure the DIP joint angle of the little finger and presence of degenerative changes in the DIP joint in Basque hand-pelota players and compare it with the general Spanish population.

Material And Methods: Cross-sectional study. We studied both hands of 40 male Basque pelota players (pelotaris) and 20 male controls. Read More

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February 2021

ABCE1 Acts as a Positive Regulator of Exogenous RNA Decay.

Viruses 2020 02 4;12(2). Epub 2020 Feb 4.

Department of Biological Chemistry, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan.

The 2'-5'-oligoadenylate synthetase (OAS)/RNase L system protects hosts against pathogenic viruses through cleavage of the exogenous single-stranded RNA. In this system, an evolutionally conserved RNA quality control factor Dom34 (known as Pelota (Pelo) in higher eukaryotes) forms a surveillance complex with RNase L to recognize and eliminate the exogenous RNA in a manner dependent on translation. Here, we newly identified that ATP-binding cassette sub-family E member 1 (ABCE1), which is also known as RNase L inhibitor (RLI), is involved in the regulation of exogenous RNA decay. Read More

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February 2020

Extraction of mRNA from Stalled Ribosomes by the Ski Complex.

Mol Cell 2020 03 31;77(6):1340-1349.e6. Epub 2020 Jan 31.

Department of Cell Biology, SUNY Downstate Health Sciences University, Brooklyn, NY, USA. Electronic address:

The evolutionarily conserved Ski2-Ski3-Ski8 (Ski) complex containing the 3'→5' RNA helicase Ski2 binds to 80S ribosomes near the mRNA entrance and facilitates 3'→5' exosomal degradation of mRNA during ribosome-associated mRNA surveillance pathways. Here, we assayed Ski's activity using an in vitro reconstituted translation system and report that this complex efficiently extracts mRNA from 80S ribosomes in the 3'→5' direction in a nucleotide-by-nucleotide manner. The process is ATP dependent and can occur on pre- and post-translocation ribosomal complexes. Read More

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Physical Exercise Is Confirmed to Reduce Low Back Pain Symptoms in Office Workers: A Systematic Review of the Evidence to Improve Best Practices in the Workplace.

J Funct Morphol Kinesiol 2019 Jul 5;4(3). Epub 2019 Jul 5.

Sport and Exercise Medicine Division, Department of Medicine, University of Padova, 35128 Padova, Italy.

This systematic review aimed to analyze the effects of a physical exercise (PE) program on low back pain (LBP) symptoms of office workers and the modification of flexibility and range of motion (ROM), muscular strength, and quality of life (QoL). A literature research was performed on PubMed, Scopus, MEDLINE, and SPORTDiscus from April to May 2018. The keyword "low back pain" was associated with "office worker" OR "VDT operators" OR "office employees" OR "workplace" AND "exercise", OR "exercise therapy" OR "physical activity". Read More

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Ribosome clearance during RNA interference.

RNA 2019 08 20;25(8):963-974. Epub 2019 May 20.

Department of MCD Biology, UC Santa Cruz, Santa Cruz, California 95064, USA.

In the course of identifying and cleaving RNA, the RNAi machinery must encounter and contend with the megadalton-sized ribosomes that carry out translation. We investigated this interface by examining the fate of actively translated mRNAs subjected to RNAi in Quantifying RNA levels (RNA-seq) and ongoing translation (Ribo-seq), we found there is a greater fold repression of ongoing translation than expected from loss of RNA alone, observing stronger translation repression relative to RNA repression for multiple, independent double-stranded RNA triggers, and for multiple genes. In animals that lack the RNA helicase SKI complex and the ribosome rescue factor PELOTA, ribosomes stall on the 3' edges of mRNAs at and upstream of the RNAi trigger. Read More

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Birthing ball versus pethidine and haloperidol in satisfaction with childbirth.

Enferm Clin 2019 Jul - Aug;29(4):234-238. Epub 2019 Mar 20.

Unidad de Apoyo a la Investigación, Hospital General Universitario Gregorio Marañón, Madrid, España.

Objective: To determine the effects on maternal satisfaction of the use of the birthing ball as a method of pain relief compared to the subcutaneous administration of pethidine (50mg) and haloperidol (2.5mg), during the latent phase of labour.

Method: Randomised, unicentric, parallel and controlled clinical trial. Read More

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Cellular response to small molecules that selectively stall protein synthesis by the ribosome.

PLoS Genet 2019 03 15;15(3):e1008057. Epub 2019 Mar 15.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, United States of America.

Identifying small molecules that inhibit protein synthesis by selectively stalling the ribosome constitutes a new strategy for therapeutic development. Compounds that inhibit the translation of PCSK9, a major regulator of low-density lipoprotein cholesterol, have been identified that reduce LDL cholesterol in preclinical models and that affect the translation of only a few off-target proteins. Although some of these compounds hold potential for future therapeutic development, it is not known how they impact the physiology of cells or ribosome quality control pathways. Read More

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Pelota-interacting G protein Hbs1 is required for spermatogenesis in Drosophila.

Sci Rep 2019 03 1;9(1):3226. Epub 2019 Mar 1.

National Institute of Biological Sciences, No. 7 Science Park Road, Zhongguancun Life Science Park, Beijing, 102206, China.

Hbs1, which is homologous to the GTPase eRF3, is a small G protein implicated in mRNA quality control. It interacts with a translation-release factor 1-like protein Dom34/Pelota to direct decay of mRNAs with ribosomal stalls. Although both proteins are evolutionarily conserved in eukaryotes, the biological function of Hbs1 in multicellular organisms is yet to be characterized. Read More

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Mammalian Hbs1L deficiency causes congenital anomalies and developmental delay associated with Pelota depletion and 80S monosome accumulation.

PLoS Genet 2019 02 1;15(2):e1007917. Epub 2019 Feb 1.

Division of Newborn Medicine, Boston Children's Hospital, Boston, Massachusetts, United States of America.

Hbs1 has been established as a central component of the cell's translational quality control pathways in both yeast and prokaryotic models; however, the functional characteristics of its human ortholog (Hbs1L) have not been well-defined. We recently reported a novel human phenotype resulting from a mutation in the critical coding region of the HBS1L gene characterized by facial dysmorphism, severe growth restriction, axial hypotonia, global developmental delay and retinal pigmentary deposits. Here we further characterize downstream effects of the human HBS1L mutation. Read More

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February 2019

Control of mRNA Translation by Versatile ATP-Driven Machines.

Trends Biochem Sci 2019 02 7;44(2):167-180. Epub 2018 Dec 7.

Institute of Biochemistry, Biocenter, Goethe University, Max von Laue-Str. 9, 60438 Frankfurt/Main, Germany. Electronic address:

Translation is organized in a cycle that requires ribosomal subunits, mRNA, aminoacylated transfer RNAs, and myriad regulatory factors. As soon as translation reaches a stop codon or stall, a termination or surveillance process is launched via the release factors eRF1 or Pelota, respectively. The ATP-binding cassette (ABC) protein ABCE1 interacts with release factors and coordinates the recycling process in Eukarya and Archaea. Read More

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February 2019

The No-go decay system degrades plant mRNAs that contain a long A-stretch in the coding region.

Plant Sci 2018 Oct 21;275:19-27. Epub 2018 Jul 21.

Agricultural Biotechnology Institute, Szent-Györgyi 4, H-2100, Gödöllő, Hungary. Electronic address:

RNA quality control systems identify and degrade aberrant mRNAs, thereby preventing the accumulation of faulty proteins. Non-stop decay (NSD) and No-go decay (NGD) are closely related RNA quality control systems that act during translation. NSD degrades mRNAs lacking a stop codon, while NGD recognizes and decays mRNAs that contain translation elongation inhibitory structures. Read More

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October 2018

Application of Whole Genome Resequencing in Mapping of a Tomato Yellow Leaf Curl Virus Resistance Gene.

Sci Rep 2018 06 25;8(1):9592. Epub 2018 Jun 25.

Institute of Vegetable Crop, Jiangsu Academy of Agricultural Science, Nanjing, Jiangsu, China.

Tomato yellow leaf curl virus (TYLCV) has significantly impacted the tomato industry around the world, and the use of insecticides and insect nets have not effectively controlled the spread of this pathogen. The tomato line AVTO1227 is highly resistant to TYLCV. In this study, F and BC populations derived from AVTO1227 and the susceptible line Money maker were used to assess the genetic mechanism underlying TYLCV resistance. Read More

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A Pelota-like gene regulates root development and defence responses in rice.

Ann Bot 2018 08;122(3):359-371

College of Science & Technology, Ningbo University, Ningbo, PR China.

Background And Aims: Pelota (Pelo) are evolutionarily conserved genes reported to be involved in ribosome rescue, cell cycle control and meiotic cell division. However, there is little known about their function in plants. The aim of this study was to elucidate the function of an ethylmethane sulphonate (EMS)-derived mutation of a Pelo-like gene in rice (named Ospelo). Read More

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The nonstop decay and the RNA silencing systems operate cooperatively in plants.

Nucleic Acids Res 2018 05;46(9):4632-4648

Agricultural Biotechnology Institute, Szent-Györgyi 4, H-2100 Gödöllo, Hungary.

Translation-dependent mRNA quality control systems protect the protein homeostasis of eukaryotic cells by eliminating aberrant transcripts and stimulating the decay of their protein products. Although these systems are intensively studied in animals, little is known about the translation-dependent quality control systems in plants. Here, we characterize the mechanism of nonstop decay (NSD) system in Nicotiana benthamiana model plant. Read More

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An evolutionarily conserved ribosome-rescue pathway maintains epidermal homeostasis.

Nature 2018 04 11;556(7701):376-380. Epub 2018 Apr 11.

Centre for Stem Cells and Regenerative Medicine, King's College London, London, UK.

Ribosome-associated mRNA quality control mechanisms ensure the fidelity of protein translation. Although these mechanisms have been extensively studied in yeast, little is known about their role in mammalian tissues, despite emerging evidence that stem cell fate is controlled by translational mechanisms. One evolutionarily conserved component of the quality control machinery, Dom34 (in higher eukaryotes known as Pelota (Pelo)), rescues stalled ribosomes . Read More

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LML1, Encoding a Conserved Eukaryotic Release Factor 1 Protein, Regulates Cell Death and Pathogen Resistance by Forming a Conserved Complex with SPL33 in Rice.

Plant Cell Physiol 2018 May;59(5):887-902

Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China.

Lesion mimic mutants are powerful tools for unveiling the molecular connections between cell death and pathogen resistance. Various proteins responsible for lesion mimics have been identified; however, the mechanisms underlying lesion formation and pathogen resistance are still unknown. Here, we identify a lesion mimic mutant in rice, lesion mimic leaf 1 (lml1). Read More

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Bta-miR-2411 attenuates bovine viral diarrhea virus replication via directly suppressing Pelota protein in Madin-Darby bovine kidney cells.

Vet Microbiol 2018 Feb 9;215:43-48. Epub 2018 Jan 9.

College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China.

MicroRNAs (miRNAs) are endogenous ∼22 nt noncoding RNAs that control the translation initiation and stability of target genes in a sequence-specific manner and, thus, play important regulatory roles in animals and plants. Homologs of Dom34, called Pelota or PELO, are broadly conserved in eukaryotes and archaea. Biochemical and genetic studies indicate that eukaryotic Dom34/Pelota plays an important role in cell division, differentiation of germline stem cells, and stem cell self-renewal by controlling the expression of specific genes at the translational level. Read More

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February 2018

Nonsense mRNA suppression via nonstop decay.

Elife 2018 01 8;7. Epub 2018 Jan 8.

Departments of Pathology and Genetics, Stanford University School of Medicine, Stanford, United States.

Nonsense-mediated mRNA decay is the process by which mRNAs bearing premature stop codons are recognized and cleared from the cell. While considerable information has accumulated regarding recognition of the premature stop codon, less is known about the ensuing mRNA suppression. During the characterization of a second, distinct translational surveillance pathway (nonstop mRNA decay), we trapped intermediates in nonsense mRNA degradation. Read More

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January 2018

A substitution mutation in OsPELOTA confers bacterial blight resistance by activating the salicylic acid pathway.

J Integr Plant Biol 2018 Feb 17;60(2):160-172. Epub 2018 Jan 17.

State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China.

We previously reported a spotted-leaf mutant pelota (originally termed HM47) in rice displaying arrested growth and enhanced resistance to multiple races of Xanthomonas oryzae pv. oryzae. Here, we report the map-based cloning of the causal gene OsPELOTA (originally termed spl ). Read More

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February 2018

Bioinformatics comparisons of RNA-binding proteins of pathogenic and non-pathogenic Escherichia coli strains reveal novel virulence factors.

BMC Genomics 2017 Aug 24;18(1):658. Epub 2017 Aug 24.

National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bellary Road, Bangalore, Karnataka, 560 065, India.

Background: Pathogenic bacteria have evolved various strategies to counteract host defences. They are also exposed to environments that are undergoing constant changes. Hence, in order to survive, bacteria must adapt themselves to the changing environmental conditions by performing regulations at the transcriptional and/or post-transcriptional levels. Read More

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Slowed decay of mRNAs enhances platelet specific translation.

Blood 2017 04 17;129(17):e38-e48. Epub 2017 Feb 17.

Department of Embryology, Carnegie Institution of Washington, Baltimore, MD; and.

Platelets are anucleate cytoplasmic fragments that lack genomic DNA, but continue to synthesize protein using a pool of messenger RNAs (mRNAs), ribosomes, and regulatory small RNAs inherited from the precursor megakaryocyte (MK). The regulatory processes that shape the platelet transcriptome and the full scope of platelet translation have remained elusive. Using RNA sequencing (RNA-Seq) and ribosome profiling of primary human platelets, we show the platelet transcriptome encompasses a subset of transcripts detected by RNA-Seq analysis of in vitro-derived MK cells and that these platelet-enriched transcripts are broadly occupied by ribosomes. Read More

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A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans.

Nucleic Acids Res 2017 02;45(4):2068-2080

The exosome complex is a major eukaryotic exoribonuclease that requires the SKI complex for its activity in the cytoplasm. In yeast, the Ski7 protein links both complexes, whereas a functional equivalent of the Ski7 has remained unknown in the human genome. Proteomic analysis revealed that a previously uncharacterized short splicing isoform of HBS1L (HBS1LV3) is the long-sought factor linking the exosome and SKI complexes in humans. Read More

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February 2017

Nonstop-mRNA decay machinery is involved in the clearance of mRNA 5'-fragments produced by RNAi and NMD in Drosophila melanogaster cells.

Biochem Biophys Res Commun 2017 02 20;484(1):1-7. Epub 2017 Jan 20.

The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan; RIBOMIC Inc., Minato-ku, Tokyo 108-0071, Japan. Electronic address:

When translating mRNAs are cleaved in protein-coding regions, 5' fragments of mRNAs are detached from stop codons (i.e., nonstop mRNAs) and protected from 3'-5' exonucleases by ribosomes stalled at the 3' termini. Read More

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February 2017

Dynamic Regulation of a Ribosome Rescue Pathway in Erythroid Cells and Platelets.

Cell Rep 2016 09;17(1):1-10

Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21218, USA; Department of Molecular Cell Biology, Center for RNA Systems Biology, Glenn Center for Aging Research, University of California Berkeley, Berkley, CA 94720, USA. Electronic address:

Protein synthesis continues in platelets and maturing reticulocytes, although these blood cells lack nuclei and do not make new mRNA or ribosomes. Here, we analyze translation in primary human cells from anucleate lineages by ribosome profiling and uncover a dramatic accumulation of post-termination unrecycled ribosomes in the 3' UTRs of mRNAs. We demonstrate that these ribosomes accumulate as a result of the natural loss of the ribosome recycling factor ABCE1 during terminal differentiation. Read More

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September 2016

Conserved functions of human Pelota in mRNA quality control of nonstop mRNA.

FEBS Lett 2016 09 7;590(18):3254-63. Epub 2016 Sep 7.

Graduate School of Pharmaceutical Science, Tohoku University, Aoba-ku, Sendai, Japan.

Dom34-Hbs1 plays crucial roles in Nonstop Decay (NSD) and No-Go Decay (NGD). Here, we report a conserved function of human Pelota (hPelota) in mRNA quality control of nonstop mRNA. hPelota facilitated the expression of the nonstop products from GFP-Rz mRNA, which lacks a termination codon and a poly(A) tail, in exosome-defective mutant cells. Read More

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September 2016

Pelota Regulates Epidermal Differentiation by Modulating BMP and PI3K/AKT Signaling Pathways.

J Invest Dermatol 2016 08 7;136(8):1664-1671. Epub 2016 May 7.

Institute of Human Genetics, University of Göttingen, D-37073 Göttingen, Germany. Electronic address:

The depletion of evolutionarily conserved pelota protein causes impaired differentiation of embryonic and spermatogonial stem cells. In this study, we show that temporal deletion of pelota protein before epidermal barrier acquisition leads to neonatal lethality due to perturbations in permeability barrier formation. Further analysis indicated that this phenotype is a result of failed processing of profilaggrin into filaggrin monomers, which promotes the formation of a protective epidermal layer. Read More

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Nutr Hosp 2015 Nov 1;32(5):2208-15. Epub 2015 Nov 1.

Department of Physiology and Biochemistry, University of Valladolid (Soria), Spain..

Introduction: there is hardly any reference in scientific literature regarding anthropometric characteristics, body composition and somatotype of hand Basque pelota players (pelotaris).

Objectives: the aim of this research was to analyze and compare the anthropometry features and body composition of professional pelotaris and under-23 (U23) pelotaris, to create an anthropometric profile of this sport.

Methods: the participants were ten U23 pelotaris, and eight professional pelotaris. Read More

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November 2015