1,302 results match your criteria pcr-rflp serum

Interleukin 4 and Interleukin 4 receptor alpha gene variants and risk of atopy - A case control study based assessment.

Clin Immunol 2021 Jun 12:108783. Epub 2021 Jun 12.

Department of Clinical Biochemistry, Sher-I-Kashmir Institute of Medical Sciences (SKIMS), Soura, 190011, J&K, India. Electronic address:

Introduction: IL4 pathway is known to upregulate IgE mediated immune responses and responsible for the manifestation of Atopic disorders. The current study was aimed to elucidate the genetic variations of Interleukin 4 (IL4) and Interleukin 4 receptor alpha (IL4R) genes and their possible association with atopic subjects.

Methods: The well-designed questionnaire was used to collect the subject demographic and clinical details. Read More

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Association of Genetic Variants in -Related Genes With Risk of Metabolic Syndrome in the Chinese Han Population.

Front Endocrinol (Lausanne) 2021 20;12:654747. Epub 2021 May 20.

Department of Endocrinology, The Affiliated Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Aims: To explore associations between polymorphisms of -related genes including , , and and Metabolic syndrome (MetS) susceptibility in the Chinese Han population.

Methods: 66 subjects with MetS and 257 control subjects were collected for inclusion in a case-control study. PCR-RFLP was used to investigate polymorphisms in the , , and genes. Read More

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The immune response and protective efficacy of a potential DNA vaccine against virulent Pasteurella multocida.

J Genet Eng Biotechnol 2021 May 31;19(1):81. Epub 2021 May 31.

Genetics Department, Faculty of Agriculture, Fayoum University, 63514, Fayoum, Egypt.

Background: Pasteurella multocida is the main cause of several infections of farm animals, and the immunity gained from commercial vaccines is for the short term only and needs to be routinely administered, so work on new vaccines against virulent P. multocida is crucial.

Results: In this study, the OmpH gene was amplified from ten P. Read More

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Significant association between Taq1 gene polymorphism in vitamin D receptor and chronic spontaneous urticaria in the Northeast of Iran.

Clin Mol Allergy 2021 May 27;19(1). Epub 2021 May 27.

Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Objective: Chronic spontaneous urticaria (CSU) is defined as urticaria with an unknown etiology which persists for more than 6 weeks. CSU is an uncomfortable cutaneous condition that occurs due to an immune-mediated inflammatory reaction. Many studies have demonstrated that vitamin D deficiency and single-nucleotide polymorphisms in the vitamin D receptor (VDR) impact the immune response. Read More

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Tumour necrosis factor-alpha polymorphism -308 G/a and its protein in subjects with gingivitis.

Acta Odontol Scand 2021 May 22:1-6. Epub 2021 May 22.

Biosciences Research Institute, Los Altos University Center, University of Guadalajara, Tepatitlán de Morelos, México.

Objective: This study examined the association between tumour necrosis factor-alpha ( α (-308 G/A) polymorphism and gingivitis, and serum and salivary TNF- α levels, in a Mexican population.

Material And Methods: This study enrolled 171 subjects, divided into two groups: healthy subjects and gingivitis patients. α (-308 G/A) gene polymorphism was analyzed by PCR-RFLP assay. Read More

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Evaluation of reproductive hormones in Egyptian workers occupationally exposed to di-2-ethylhexyl phthalate (DEHP): a cross-sectional study.

J Complement Integr Med 2021 May 18. Epub 2021 May 18.

Environmental and Occupational Medicine Department, Environmental Research Division, National Research Centre, Cairo, Egypt.

Objectives: Di-2-ethylhexyl phthalate (DEHP) is ubiquitous, known as an endocrine disruptor. DEHP is a widespread prevalence in general and occupational populations which raised great public concerns due to its potentially harmful health effects on the male reproductive system. We aimed to assess occupational levels of DEHP on gonadotropin and gonadal hormones including luteinizing hormone (LH), follicle-stimulating hormone (FSH), total testosterone (TT), and sex hormone binding globulin (SHBG) and evaluate its potential effects on Asp327Asn polymorphisms SHBG gene. Read More

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The role of caveolin-1 and endothelial nitric oxide synthase polymorphisms in susceptibility to prostate cancer.

Int J Exp Pathol 2021 May 8. Epub 2021 May 8.

Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Caveolin-1(cav-1) is overexpressed in prostate cancer (PC) and is associated with progression of the disease. We investigated the effects of CAV1-T29107A and endothelial nitric oxide synthase (eNOS) G894T polymorphisms on the serum levels of testosterone, NO and prostate-specific antigen (PSA) in patients with PC. We genotyped cav-1 and eNOS genes in 112 PC patients and 150 healthy controls by PCR-RFLP. Read More

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TT genotype of the MMP-9-1562C/T polymorphism may be a risk factor for thrombolytic therapy-induced hemorrhagic complications after acute ischemic stroke.

Pharmacotherapy 2021 May 4. Epub 2021 May 4.

Euromedic Hospital, Belgrade, Serbia.

Introduction: Levels of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) influence recombinant tissue plasminogen activator (rtPA) therapy response in patients with acute ischemic stroke (AIS). Serum levels of MMPs and TIMPs along with the expression of genes coding these proteins are related to the recovery and appearance of adverse effects (AE) after AIS. Consequently, it is important to explore whether polymorphisms in regulatory sequences of MMPs and TIMPs are associated with rtPA response in AIS patients. Read More

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Interaction between the dietary indices and PPAR-γ Pro12Ala gene variants on cardiovascular risk factors in patients with type 2 diabetes mellitus.

Int J Clin Pract 2021 Apr 30:e14307. Epub 2021 Apr 30.

Department of Cellular, Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences (TUMS), Tehran, Iran.

Aims: We investigated the interaction between peroxisome proliferator-activated receptor gamma (PPAR-γ) Pro12Ala polymorphism and healthy eating index (HEI), Dietary Quality Index-International (DQI-I), and dietary phytochemical index (DPI) on cardiovascular disease (CVD) risk factors in patients with type 2 diabetes mellitus (T2DM).

Methods: This cross-sectional study was conducted on 393 diabetic patients. PPAR-γ Pro12Ala was genotyped by the PCR-RFLP method. Read More

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Increased homocysteine mediated oxidative stress as key determinant of hepatitis E virus (HEV) infected pregnancy complication and outcome: A study from Northeast India.

Infect Genet Evol 2021 Aug 24;92:104882. Epub 2021 Apr 24.

Department of Biotechnology, Gauhati University, Guwahati, Assam 781014, India. Electronic address:

With the background of association of oxidative stress and Hepatitis E virus (HEV) infection in pregnancy complications the present novel study aimed to evaluate the significance of changes in maternal homocysteine levels and the related mechanism(s) in the pathophysiology of HEV related pregnancy complications and negative outcomes. Term delivery (TD, N = 194) and HEV-IgM positive pregnancy cases [N = 109] were enrolled. Serum and placental homocysteine levels were evaluated by ELISA and immunofluorescence and in turn correlated with serum Vitamin B12 levels. Read More

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Vitamin D Levels and Vitamin D Receptor (VDR) Gene Polymorphisms in Inactive Hepatitis B Virus Carriers.

J Coll Physicians Surg Pak 2021 Apr;30(4):393-398

Department of Medical Biology, Faculty of Medicine, Katip Celebi University, Ataturk Training and Research Hospital, Izmir, Turkey.

Objective: To evaluate the vitamin D receptor (VDR) gene polymorphisms and vitamin D levels in inactive hepatitis B virus (HBV) carriers.

Study Design: A cross-sectional analytical study.

Place And Duration Of Study: From March to September 2017 at the Izmir Katip Celebi University (İKCU) Ataturk Training and Research Hospital, Izmir, Turkey. Read More

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Relation of vitamin D and BsmI variant with temporomandibular diseases in the Turkish population.

Br J Oral Maxillofac Surg 2021 Jun 28;59(5):555-560. Epub 2020 Aug 28.

Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Gaziosmanpasa University, Tokat, Turkey. Electronic address:

Vitamin D (VD) levels and several variants in the vitamin D receptor (VDR) gene are associated with the occurrence of diseases of the bones and cartilage. The aim of this research was to study and compare the association of the BsmI variant in the VDR gene as well as VD levels in disc displacement with reduction (DDR) between patients and healthy controls. This was a case-control study, in which 104 patients of DDR and 102 healthy individuals were studied. Read More

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Gene polymorphisms in leptin and its receptor and the response to growth hormone treatment in patients with idiopathic growth hormone deficiency.

Endocr J 2021 Mar 24. Epub 2021 Mar 24.

Department of Pediatrics, Hallym University College of Medicine, 1, Hallymdaehak-gil, Chuncheon-si, Gangwon-do, 24252, Republic of Korea.

This study aimed to investigate the relationships between genetic polymorphisms of leptin/receptor genes and clinical/biochemical characteristics in children with growth hormone deficiency (GHD). Ninety-three GHD children and 69 age-matched normal controls were enrolled. Anthropometric measurements, bone age, and laboratory test results were obtained. Read More

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Association between Interleukin-1β Polymorphism at Rs16944 and Glucose Metabolism: A Cohort Study.

Immunol Invest 2021 Mar 19:1-11. Epub 2021 Mar 19.

Department of Endocrinology, The Affiliated Hospital of Southwest Medical University, Luzhou, China.

: This study explored the correlation between the interleukin-1β gene rs16944 polymorphism and diabetes through epidemiological and follow-up investigations.: The study was conducted on 600 subjects with normal glucose metabolism recruited from participants of the in Luzhou, China in 2011. All subjects received a unified standardized questionnaire, physical examination, laboratory examination, and follow-up in 2016. Read More

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Toxoplasma gondii infection in wild boars (Sus scrofa) from the State of São Paulo, Brazil: Serology, molecular characterization, and hunter's perception on toxoplasmosis.

Vet Parasitol Reg Stud Reports 2021 Jan 19;23:100534. Epub 2021 Jan 19.

Universidade Estadual Paulista - Unesp, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, Departamento de Patologia, Reprodução Animal e Saúde Única, Rodovia Professor Paulo Donato Castelane, s/n, Zona Rural, CEP 14887-900, Jaboticabal, São Paulo, Brazil. Electronic address:

The consumption of wild boar meat, common in many countries, became popular in Brazil after the hunting of these animals was authorized in 2013. The meat of these animals is often consumed by hunters and their social groups, and their offal is occasionally used as supplemental food in the diet of hunting dogs. Given the high frequency of foodborne diseases related to wild boar meat consumption in other countries, including toxoplasmosis, knowledge on these diseases is essential for risk assessment and elaboration of education campaigns for the exposed public. Read More

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January 2021

Interleukin-27 gene variant rs153109 is associated with enhanced cytokine serum levels and susceptibility to Behçet's disease in the Iranian population.

Eur Cytokine Netw 2020 Dec;31(4):140-146

Autoimmune Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran.

Behcet's disease (BD) is a systemic vasculitis, characterized by recurrent oral aphthous, genital ulcers, ocular lesions, and other organ involvement. Interleukin (IL)-27 with its pro- and anti-inflammatory effects might be an important effective cytokine in this disease. The aim of this study was to investigate the association of IL-27 serum concentration and a single-nucleotide polymorphism (SNP) rs153109 (-964 A > G) with the risk and clinical features of the patients with BD. Read More

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December 2020

Interferon-γ inducible protein-10 and interleukin 28B gene polymorphism as predictive markers for genotype 4 hepatitis C virus treatment response.

Trop Biomed 2020 Dec;37(4):1083-1092

Biochemistry Department, Faculty of Science, Ain Shams University, Abbassia, 11566, Cairo, Egypt.

HCV genotype 4 dominates the HCV epidemic in Egypt. Drug resistance was the most serious side effect that reflects bad clinical outcome. Several studies had demonstrated that baseline serum interferon-γ-inducible-protein 10 (IP-10) levels and interleukin 28B polymorphisms were associated with the resistance to the standard of care pegylated interferon alpha and ribavirin (PEG-IFNα/RBV) therapy and development of post-treatment relapse. Read More

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December 2020

Impact of cathepsin D activity and C224T polymorphism (rs17571) on chronic obstructive pulmonary disease: correlations with oxidative and inflammatory markers.

Clin Exp Med 2021 Feb 21. Epub 2021 Feb 21.

Laboratoire de Recherche LR19ES09, Physiologie de L'Exercice Et Physiopathologie : de L'Intégré Au Moléculaire Biologie, Médecine Et Santé, Faculté de Médecine de Sousse, Université de Sousse, Sousse, Tunisia.

Background: Cathepsin D (CTSD) is an aspartyl proteinase that plays an important role in protein degradation, antigen processing and apoptosis. It has been associated with several pathologies such as cancer, Alzheimer's disease and inflammatory disorders. Its function in lung diseases remains, however, controversial. Read More

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February 2021

Vitamin D status and CYP27B1-1260 promoter polymorphism in Tunisian patients with systemic lupus erythematosus.

Mol Genet Genomic Med 2021 Mar 17;9(3):e1618. Epub 2021 Feb 17.

Autoimmunity, Cancer and immunogenetics research laboratory, University hospital Habib Bourguiba of Sfax, Sfax, Tunisia.

Aim: An association between serum vitamin D (Vit D) levels and systemic lupus erythematosus (SLE) has been reported by several studies that suggested the involvement of genetically determined characteristics of enzymes of vitamin D metabolism. Our study aimed to evaluate the relationship between 25 hydroxyvitamin D (25[OH]D) level, the most representative metabolite of VitD status, and polymorphism of the cytochrome P450, CYP27B1 gene, which influence vitamin D metabolism, and serum levels, in SLE Tunisian patients.

Material And Methods: A cross-sectional study has been conducted in SLE patients (supplemented and not supplemented patients), matched to healthy controls by age and gender. Read More

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Correlation study of CYP2C19 gene polymorphism and clopidogrel resistance in Han Chinese patients with cerebral infarction in Guizhou region.

Medicine (Baltimore) 2021 Feb;100(6):e24481

Department of Neurology, Guizhou Aerospace Hospital, Zunyi.

Abstract: This study conducts a correlation exploration of CYP2C19 gene polymorphism and clopidogrel resistance in Han Chinese patients with cerebral infarction in Guizhou Region.A total of 270 Han Chinese patients with cerebral infarction, who were hospitalized in our hospital from January 2016 to January 2018, are selected. These patients were divided into 2 groups, clopidogrel resistance group (n = 60) and clopidogrel sensitive group (n = 210). Read More

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February 2021

Association of Genetic Polymorphisms and Serum Levels of IL-6 and IL-8 with the Prognosis in Children with Neuroblastoma.

Cancers (Basel) 2021 Jan 30;13(3). Epub 2021 Jan 30.

Subdirección de Pediatría Ambulatoria Hospital Infantil de México Federico Gómez, Dr. Márquez No. 162, Col Doctores, Delegación Cuauhtémoc, Ciudad de Mexico 06720, Mexico.

There is evidence that high circulating levels of IL-6 and IL-8 are markers of a poor prognosis in various types of cancer, including NB. The participation of these cytokines in the tumor microenvironment has been described to promote progression and metastasis. Our objective was to evaluate the prognostic role of genetic polymorphisms and serum levels of IL-6 and IL-8 in a cohort of Mexican pediatric patients with NB. Read More

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January 2021

Association of serum levels and receptor genes BsmI, TaqI and FokI polymorphisms of vitamin D with the severity of multiple sclerosis.

J Clin Neurosci 2021 Feb 29;84:75-81. Epub 2020 Dec 29.

Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran; Department of Immunology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran. Electronic address:

Purpose: Multiple sclerosis (MS) is a chronic inflammatory and neurodegenerative disease. Vitamin D has a major role in preventing inflammatory disorders. Therefore, any alteration in vitamin D receptor (VDR) might be a genetic risk factor for MS development. Read More

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February 2021

Non-classical human leukocyte antigen class I in Tunisian children with autism.

Cent Eur J Immunol 2020 27;45(2):176-183. Epub 2020 Jul 27.

Laboratory Microorganismes and Active Biomolecules, Sciences Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia.

Autism spectrum disorders (ASD) are one of the most common childhood morbidities characterized by deficits in communication and social skills. Increasing evidence has suggested associations between immune genes located in the human leukocyte antigen (HLA) complex and etiology of autism. In this study, we investigated whether the non-classical class I HLA-G, -E, and -F polymorphisms are associated with genetic predisposition to autism in Tunisia. Read More

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The Impact of Kinase Insert Domain (KDR) Gene Polymorphism rs2305948 on Clopidogrel Resistance in Iraqi Patients Undergoing Elective Percutaneous Coronary Intervention (PCI).

Acta Inform Med 2020 Sep;28(3):202-208

Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Kufa.

Introduction: Clopidogrel, the first-choice antiplatelet agent for patient undergoing Percutaneous Coronary Intervention (PCI) along with Aspirin. Clopidogrel resistance is one of the major obstacles that cause MACE and failure of PCI. Kinase Insert Domain (KDR) gene responsible for VEGFR2 coding, the major receptor that translates VEGF ligand. Read More

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September 2020

IL-17A polymorphism (rs2275913) and levels are associated with preeclampsia pathogenesis in Chinese patients.

BMC Med Genomics 2021 01 6;14(1). Epub 2021 Jan 6.

The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, 910 Hengshan Road, Shanghai, 200030, China.

Background: Preeclampsia (PE) is a pregnancy-related condition that affects both the infant and the mother. Although the role of various inflammatory molecules in PE has been demonstrated, the importance of pro-inflammatory molecules such as IL-17A, IL-23 is not well understood. In the present investigation, a potential association of common genetic variants in the IL-17A and IL-23A genes with PE was investigated. Read More

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January 2021

UGT1A6 and UGT2B7 Gene Polymorphism and its Effect in Pediatric Epileptic Patients on Sodium Valproate Monotherapy.

Indian J Pediatr 2021 Jan 5. Epub 2021 Jan 5.

Department of Pharmacology, Pediatrics & Biochemistry, K S Hegde Medical Academy, Nitte (Deemed to be University), Derlakatte, Mangalore, Karnataka, India.

Objectives: To evaluate the pattern of UGT1A6 and UGT2B7 gene polymorphism in pediatric epileptic patients and to compare the sodium valproate concentration in different patterns of UGT gene polymorphism.

Methods: In this cross-sectional study, 99 pediatric epileptic patients aged 2-18 y receiving Sodium valproate monotherapy for the past one month were included from JusticeK S Hegde Charitable hospital, Mangalore after obtaining informed consent. Genetic polymorphism patterns were evaluated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Read More

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January 2021

Exploring the Role of C-C Motif Chemokine Ligand-2 Single Nucleotide Polymorphism in Pulmonary Tuberculosis: A Genetic Association Study from North India.

J Immunol Res 2020 16;2020:1019639. Epub 2020 Dec 16.

Immunology Division, National JALMA Institute for Leprosy and Other Mycobacterial Diseases (Indian Council of Medical Research), Dr Miyazaki Marg, TajGanj, 282 004, Agra, India.

The C-C motif chemokine ligand-2 (CCL2) was evidenced to be associated with tuberculosis susceptibility in some ethnic groups. In the present study, effort was made to find out the association of -2518 A>G and -362 G>C variants with susceptibility to TB in a population from North India. The genotyping was carried out in 373 participants with pulmonary TB (PTB) and 248 healthy controls (HCs) for 2518 A>G and -362 G>C polymorphisms by PCR-RFLP and by melting curve analysis using fluorescence-labeled hybridization fluorescent resonance energy transfer (FRET) probes, respectively, followed by DNA sequencing in a few representative samples. Read More

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December 2020

The Influence of Vitamin D Receptor Gene Polymorphisms in Spondyloarthritis.

Int J Inflam 2020 8;2020:8880879. Epub 2020 Dec 8.

Biosciences and Physiopathology, Department of Clinical Analysis and Biomedicine, Maringá State University, Paraná, Brazil.

Spondyloarthritis (SpA) is an inflammatory rheumatic disease related to low bone mineral density. Because vitamin D plays an important role in bone metabolism and immune system modulation, the aim of this study was to evaluate the influence of polymorphisms in vitamin D receptor genes () in the development of SpA. In this case-control study, a total of 244 patients with SpA and 197 individuals with no SpA were included. Read More

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December 2020

Association of and Gene Variants with Plasma Levels and Risk of Vulvovaginal Candidiasis in a Chinese Han Population.

Pharmgenomics Pers Med 2020 15;13:725-733. Epub 2020 Dec 15.

Department of Obstetrics and Gynecology, Taizhou People's Hospital, Taizhou, People's Republic of China.

Background: Vulvovaginal candidiasis (VVC) is a common vaginal inflammatory disease in females. The interleukin (IL)-23/IL-17 axis was involved in vaginal inflammation. Nevertheless, the relationship between gene polymorphisms in the IL-23/IL-17 axis and VVC risk is still unexplored. Read More

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December 2020

Correlation of sexual dysfunction with sex hormone and estrogen receptor gene polymorphism in Chinese Han women with epilepsy.

Epilepsy Res 2021 Jan 10;169:106527. Epub 2020 Dec 10.

Department of Neurology, The Affiliated Hospital of Yangzhou University, No. 368, Hanjiang Road, Yangzhou, Jiangsu 225012, China. Electronic address:

Objective: Women with epilepsy (WWE) have a high risk of sexual dysfunction (SD). We aimed to investigate the incidence of SD, the correlation between SD, sex hormone and estrogen receptor (ER) gene polymorphism in Chinese Han WWE.

Methods: This cross-sectional study examined 112 married WWE in the Affiliated Hospital of Yangzhou University who were taking antiepileptic drugs (AEDs) for ≥1 year, and 120 healthy controls without epilepsy, all of Chinese Han nationality. Read More

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January 2021