28,078 results match your criteria pcr testing

A retrospective evaluation of the value of COVID-19 screening and testing in patients with cancer: Aiming at a moving target.

J Infect Public Health 2021 May 26;14(7):949-953. Epub 2021 May 26.

Department of Oncology, King Abdulaziz Medical City, King Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs (MNGHA), Riyadh, Saudi Arabia.

Background: Diagnosis of COVID-19 infection in cancer patients is critical to co-manage their underlying disease and infection appropriately. Our study aimed at evaluating the sensitivity and specificity of screening patients with cancer for COVID-19 infection.

Methods: All oncology patients receiving care at Department of Oncology at King Abdulaziz Medical City in Riyadh were screened using the acute respiratory infection (ARI) survey. Read More

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Efficient and effective single-step screening of individual samples for SARS-CoV-2 RNA using multi-dimensional pooling and Bayesian inference.

J R Soc Interface 2021 Jun 16;18(179):20210155. Epub 2021 Jun 16.

Department of Chemistry, University of Utah, Salt Lake City, UT, USA.

Rapid and widespread implementation of infectious disease surveillance is a critical component in the response to novel health threats. Molecular assays are the preferred method to detect a broad range of viral pathogens with high sensitivity and specificity. The implementation of molecular assay testing in a rapidly evolving public health emergency, such as the ongoing COVID-19 pandemic, can be hindered by resource availability or technical constraints. Read More

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SARS-CoV-2 screening among people living in homeless shelters in Brussels, Belgium.

PLoS One 2021 15;16(6):e0252886. Epub 2021 Jun 15.

Research Center in Epidemiology, Biostatistics and Clinical Research, School of Public Health, Université Libre de Bruxelles (ULB), Brussels, Belgium.

Background: Subgroups of precarious populations such as homeless people are more exposed to infection and at higher risk of developing severe forms of COVID-19 compared to the general population. Many of the recommended prevention measures, such as social distancing and self-isolation, are not feasible for a population living in shelters characterised by physical proximity and a high population density. The objective of the study was to describe SARS-CoV-2 infection prevalence in homeless shelters in Brussels (Belgium), and to identify risk factors and infection control practices associated with SARS-CoV-2 positivity rates. Read More

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Laboratory evaluation of the rapid diagnostic tests for the detection of Vibrio cholerae O1 using diarrheal samples.

PLoS Negl Trop Dis 2021 Jun 15;15(6):e0009521. Epub 2021 Jun 15.

Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, India.

Background: Cholera, an acute diarrheal disease is a major public health problem in many developing countries. Several rapid diagnostic tests (RDT) are available for the detection of cholera, but their efficacies are not compared in an endemic setting. In this study, we have compared the specificity and sensitivity of three RDT kits for the detection of Vibrio cholerae O1 and compared their efficiency with culture and polymerase chain reaction (PCR) methods. Read More

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Validation of a rapid antigen test as a screening tool for SARS-CoV-2 infection in asymptomatic populations. Sensitivity, specificity and predictive values.

EClinicalMedicine 2021 Jun 9:100954. Epub 2021 Jun 9.

IdiSNA (Instituto de Investigación Sanitaria de Navarra), Pamplona, Spain.

Background: Early diagnosis of SARS-CoV-2 infection is essential to reduce disease spread. Rapid antigen tests have not been sufficiently evaluated in asymptomatic patients to be used as massive population screening tools.

Methods: Head-to-head evaluation of Roche SARS-CoV-2 Rapid Antigen Test and real-time reverse transcription polymerase chain reaction (RT-PCR) as SARS-CoV-2 screening tools performed in asymptomatic adults from a semi-closed community in University of Navarra (Spain) from November 2020 to January 2021. Read More

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Exploring antimicrobial resistance to beta-lactams, aminoglycosides and fluoroquinolones in E. coli and K. pneumoniae using proteogenomics.

Sci Rep 2021 Jun 14;11(1):12472. Epub 2021 Jun 14.

Department of Neurology, Neuro-Oncology Laboratory/Clinical and Cancer Proteomics, Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands.

Antimicrobial resistance is mostly studied by means of phenotypic growth inhibition determinations, in combination with PCR confirmations or further characterization by means of whole genome sequencing (WGS). However, the actual proteins that cause resistance such as enzymes and a lack of porins cannot be detected by these methods. Improvements in liquid chromatography (LC) and mass spectrometry (MS) enabled easier and more comprehensive proteome analysis. Read More

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Sensitive detection and quantification of SARS-CoV-2 in saliva.

Sci Rep 2021 06 14;11(1):12425. Epub 2021 Jun 14.

Section of Hematology and Oncology, Pritzker School of Medicine, University of Chicago, Chicago, IL, USA.

Saliva has significant advantages as a test medium for detection of SARS-CoV-2 infection in patients, such as ease of collection, minimal requirement of supplies and trained personnel, and safety. Comprehensive validation in a large cohort of prospectively collected specimens with unknown SARS-CoV-2 status should be performed to evaluate the potential and limitations of saliva-based testing. We developed a saliva-based testing pipeline for detection of SARS-CoV-2 nucleic acids using real-time reverse transcription PCR (RT-PCR) and droplet digital PCR (ddPCR) readouts, and measured samples from 137 outpatients tested at a curbside testing facility and 29 inpatients hospitalized for COVID-19. Read More

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Cross sectional study of the clinical characteristics of French primary care patients with COVID-19.

Sci Rep 2021 06 14;11(1):12492. Epub 2021 Jun 14.

Primary Care Unit, Faculty of Medicine, University of Geneva, Geneva, Switzerland.

The early identification of patients suffering from SARS-CoV-2 infection in primary care is of outmost importance in the current pandemic. The objective of this study was to describe the clinical characteristics of primary care patients who tested positive for SARS-CoV-2. We conducted a cross-sectional study between March 24 and May 7, 2020, involving consecutive patients undergoing RT-PCR testing in two community-based laboratories in Lyon (France) for a suspicion of COVID-19. Read More

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Green Synthesis of Ciprofloxacin-Loaded Cerium Oxide/Chitosan Nanocarrier and Its Activity Against MRSA-Induced Mastitis.

J Pharm Sci 2021 Jun 11. Epub 2021 Jun 11.

Departments of Nanomedicine, California Innovations Corporation, San Diego, CA, USA. Electronic address:

Methicillin-resistant Staphylococcus aureus (MRSA)-induced mastitis is one of the biggest animal welfare issues and economic burdens worldwide. As a possible effective treatment, ciprofloxacin (CIP)-loaded cerium oxide (CeO)/chitosan (CS) nanocomposite was synthesized using an eco-friendly approach, characterized, and evaluated. From 350 mastitis-positive milk samples, 35 mecA-positive MRSA strains were confirmed by antibiotic sensitivity testing and PCR. Read More

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Expression of long non-coding RNA LUCAT1 in patients with chronic obstructive pulmonary disease and its potential functions in regulating cigarette smoke extract-induced 16HBE cell proliferation and apoptosis.

J Clin Lab Anal 2021 Jun 14:e23823. Epub 2021 Jun 14.

Department of Blood Transfusion, The First Affiliated Hospital of Soochow University, Suzhou, China.

Background: Chronic obstructive pulmonary disease (COPD), characterized by persistent airflow limitation, was a disease mediated by a combination of inflammatory factors, immune cells, and immune mediators. COPD was an inflammatory and autoimmune disease involving T-lymphocytes triggered by cigarette smoke and other factors that progressively affected the bronchi, lung parenchyma, and pulmonary blood vessels. LncRNAs were reported to be implicated in COPD pathogenesis and development. Read More

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Short report: The potential of PCR on skin flakes from bed linens for diagnosis of scabies in an outbreak.

PLoS Negl Trop Dis 2021 Jun 14;15(6):e0009485. Epub 2021 Jun 14.

Public Health Service of the region of Rotterdam-Rijnmond (GGD Rotterdam-Rijnmond), Rotterdam, the Netherlands.

Background: Scabies outbreaks are common in nursing homes in the Netherlands. In October 2018, a local public health service (PHS) in The Hague was notified of a new scabies outbreak in a nursing home in that region. The PHS initiated an outbreak investigation. Read More

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Optimizing SARS-CoV-2 Surveillance in the United States: Insights From the National Football League Occupational Health Program.

Ann Intern Med 2021 Jun 15. Epub 2021 Jun 15.

National Football League, New York, New York (E.M., G.S., A.S.).

Background: Evidence to understand effective strategies for surveillance and early detection of SARS-CoV-2 is limited.

Objective: To describe the results of a rigorous, large-scale COVID-19 testing and monitoring program.

Design: The U. Read More

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Rapid detection of human origin colistin-resistance genes mcr-1, mcr-3, mcr-8, mcr-10 in clinical fecal samples.

Arch Microbiol 2021 Jun 14. Epub 2021 Jun 14.

Key Laboratory of Molecular Epidemiology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Guangdong Province, No. 8 Longyuan Road, Nanshan district, Shenzhen City, 518055, People's Republic of China.

Plasmid-mediated colistin-resistance genes have been reported in human origin clinical samples worldwide which raises its threats to human infections. Notably, mcr-1, mcr-3, mcr-8, and mcr-10 have been reported isolated directly from clinical samples which creates more seriously threaten to human health than other mcr gene types. A multiplex polymerase chain reaction (Multi-PCR) protocol was developed to detect and genotype mobile colistin-resistance genes (mcr-1, mcr-3, mcr-8, mcr-10) in Enterobacteria for clinical laboratory purposes. Read More

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Clinical Prediction Tool to Assess the Likelihood of a Positive SARS-Cov-2 (COVID-19) Polymerase Chain Reaction Test in Patients with Flu-like Symptoms.

West J Emerg Med 2021 Mar 24;22(3):592-598. Epub 2021 Mar 24.

Pontificia Universidad Católica de Chile, Section of Emergency Medicine, Santiago, Chile.

Introduction: The clinical presentation of coronavirus disease 2019 (COVID-19) overlaps with many other common cold and influenza viruses. Identifying patients with a higher probability of infection becomes crucial in settings with limited access to testing. We developed a prediction instrument to assess the likelihood of a positive polymerase chain reaction (PCR) test, based solely on clinical variables that can be determined within the time frame of an emergency department (ED) patient encounter. Read More

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Utility Of An Emergency Department Clinical Protocol For Early Identification of Coronavirus Infection.

West J Emerg Med 2021 Apr 5;22(3):587-591. Epub 2021 Apr 5.

Mount Sinai Medical Center, Department of Emergency Medicine, New York City, New York.

Introduction: We assessed the utility of an emergency department (ED) protocol using clinical parameters to rapidly distinguish likelihood of novel coronavirus 2019 (COVID-19) infection; the applicability aimed to stratify infectious-risk pre-polymerase chain reaction (PCR) test results and accurately guide early patient cohorting decisions.

Methods: We performed this prospective study over a two-month period during the initial surge of the 2020 COVID-19 pandemic in a busy urban ED of patients presenting with respiratory symptoms who were admitted for in-patient care. Per protocol, each patient received assessment consisting of five clinical parameters: presence of fever; hypoxia; cough; shortness of breath/dyspnea; and performance of a chest radiograph to assess for bilateral pulmonary infiltrates. Read More

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Provider Antibody Serology Study of Virus in the Emergency Room (PASSOVER) Study: Special Population COVID-19 Seroprevalence.

West J Emerg Med 2021 Apr 9;22(3):565-571. Epub 2021 Apr 9.

Children's Hospital of Orange County, Research Institute, Orange, California.

Introduction: Limited data on the seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) among healthcare workers (HCW) are publicly available. In this study we sought to determine the seroprevalence of SARS-CoV-2 in a population of HCWs in a pediatric emergency department (ED).

Methods: We conducted this observational cohort study from April 14-May 13, 2020 in a pediatric ED in Orange County, CA. Read More

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Prevalence of SARS-CoV-2 in urban and rural Ethiopia: Randomized household serosurveys reveal level of spread during the first wave of the pandemic.

EClinicalMedicine 2021 May 7;35:100880. Epub 2021 May 7.

Bacterial, Parasitic and Zoonotic Diseases Research Directorate, Ethiopian Public Health Institute, Gulelle Arbegnoch street, Addis Ababa, Ethiopia.

Background: The spread of SARS-CoV-2 in Sub-Saharan Africa is poorly understood and to date has generally been characterised by a lower number of declared cases and deaths as compared to other regions of the world. Paucity of reliable information, with insights largely derived from limited RT-PCR testing in high-risk and urban populations, has been one of the biggest barriers to understanding the course of the pandemic and informed policy-making. Here we estimate seroprevalence of anti-SARS-CoV-2 antibodies in Ethiopia during the first wave of the pandemic. Read More

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A collection of the novel coronavirus (COVID-19) detection assays, issues, and challenges.

Heliyon 2021 Jun 6;7(6):e07247. Epub 2021 Jun 6.

Center for Nanotechnology and Sustainability, Department of Mechanical Engineering, National University of Singapore, Singapore, 117581, Singapore.

The global pandemic of COVID-19 has rapidly increased the number of infected cases as well as asymptomatic individuals in many, if not all the societies around the world. This issue increases the demand for accurate and rapid detection of SARS-CoV-2. While accurate and rapid detection is critical for diagnosing SARS-CoV-2, the appropriate course of treatment must be chosen to help patients and prevent its further spread. Read More

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COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR.

Bio Protoc 2021 May 5;11(9):e4005. Epub 2021 May 5.

Department of Community Medicine, School of Medicine, International Medical University, Kuala Lumpur, Malaysia.

The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and requires a lot of resources. Sample pooling methods improve the efficiency of mass screening and consume less reagents by increasing the capacity of testing and reducing the number of experiments performed, and are therefore especially suitable for under-developed countries with limited resources. Read More

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Assessment of Successful qRT-PCR of SARS-CoV-2 Assay in Pool Screening Using Isopropyl Alcohol Purification Step in RNA Extraction.

Biomed Res Int 2021 8;2021:6653950. Epub 2021 Jun 8.

Viral Research and Diagnostic Laboratory, Department of Microbiology, Faculty of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.

The study is aimed at establishing the optimal parameters for RNA purification of pooled specimens, in SARS-CoV-2 assay. This research work evaluates the difference of extracted RNA purity of pooled samples with and without treatment with isopropyl alcohol and its effect on real-time RT-PCR. As per the protocol of the Indian Council of Medical Research (ICMR), 5 sample pools were analysed using qRT-PCR. Read More

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Initial PCR Testing Negative, but Chest CT Suggesting for Viral Pneumonia Urges for Repeated Testing for COVID-19 Diagnosis.

Front Mol Biosci 2021 26;8:640788. Epub 2021 May 26.

The Department of Respiratory Diseases and Critic Care Unit, Shenzhen Institute of Respiratory Diseases, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, China.

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Blood Culture-negative Endocarditis Caused by : Whipple's endocarditis.

Eur J Case Rep Intern Med 2021 19;8(5):002596. Epub 2021 May 19.

Department of Cardiology, Hospital Group Twente (ZGT), Almelo and Hengelo, The Netherlands.

Case Description: A 67-year-old man was admitted with progressive heart failure due to blood culture-negative endocarditis of the aortic valve. Urgent aortic valve replacement was needed. Polymerase chain reaction (PCR) testing of samples of the explanted aortic valve revealed . Read More

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Limited role of GeneXpert in peritoneal fluid in the diagnosis of genital tuberculosis in infertile women.

J Family Med Prim Care 2021 Apr 29;10(4):1656-1660. Epub 2021 Apr 29.

Department of Statistics, Lucknow University, Lucknow, Uttar Pradesh, India.

Background And Objectives: Genital tuberculosis (TB) is an important cause of infertility in women that poses many challenges in diagnosis. The study is done to understand the utility of GeneXpert test in peritoneal fluid in the diagnosis of genital TB in infertile women.

Methods: All infertile women in postmenstrual phase who were planned for laparoscopy in study period were included. Read More

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Optimizing the Management of Cancer Patients Treated With Systemic Therapies During the COVID-19 Pandemic: The New Role of PCR and CT Scan.

Front Oncol 2021 28;11:560585. Epub 2021 May 28.

Breast Unit, Department of Medicine, Gustave Roussy Cancer Campus, Villejuif, France.

In late 2019 and early 2020, the world witnessed the outbreak of the SARS-CoV-2 (also referred as COVID-19) in Wuhan, China. Its rapid expansion worldwide and its contagiousness rate have forced the activation of several measures to contain the pandemic, mostly through confinement and identification of infected patients and potential contacts by testing. Read More

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HER2 Testing Characteristics Can Predict Residual Cancer Burden following Neoadjuvant Chemotherapy in HER2-Positive Breast Cancer.

Int J Breast Cancer 2021 24;2021:6684629. Epub 2021 May 24.

Allina Health System, Virginia Piper Cancer Institute Clinical Research Program, 800 East 28th Street, Minneapolis, MN 55407, USA.

Objectives: The response to HER2-targeted neoadjuvant chemotherapy (NAC) in HER2-positive (+) breast cancer can be quantified using residual cancer burden (RCB) pathologic evaluation to predict relapse free/overall survival. However, more information is needed to characterize the relationship between patterns of HER2 testing results and response to NAC. We evaluated clinicopathologic characteristics associated with RCB categories in HER2+ patients who underwent HER2-directed NAC. Read More

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Low seroprevalence of SARS-CoV-2 antibodies in a liver transplant cohort.

Transpl Int 2021 Jun 14. Epub 2021 Jun 14.

Liver Unit. Hospital Universitari Vall d'Hebron, Vall d'Hebron Research Institute (VHIR). Vall d'Hebron Barcelona Hospital Campus, Barcelona, Spain.

Solid organ transplant recipients might be at greater risk for acquisition and mortality due to SARS-CoV-2. There is no data regarding SARS-CoV-2 seroprevalence among liver transplant (LT) recipients, and whether it is different from that of the general population or other immunosuppressed groups. We evaluated the prevalence of IgG SARS-CoV-2 antibodies among LT recipients to estimate the frequency of asymptomatic SARS-CoV-2 infection using serological assays in our outpatient clinic. Read More

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MS device: smartphone-facilitated mobile nucleic acid analysis on microfluidic device.

Analyst 2021 Jun;146(12):3823-3833

Anhui Institute of Optics and Fine Mechanics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, PR China.

Mobile sensing based on the integration of microfluidic devices and smartphones, so-called MS2 technology, has enabled many applications over recent years and continues to stimulate growing interest in both research communities and industries. In particular, MS2 technology has been proven to be able to be applied to molecular diagnostic analysis and can be implemented for basic research and clinical testing. However, the currently reported MS2-based nucleic acid analysis system has limited use in practical applications, because it is not integrated with quantitative PCR, multiplex PCR, and isothermal amplification functions, and lacks temperature control, image acquisition and real-time processing units with excellent performance. Read More

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A simple and selective electrochemical magneto-assay for sea lice eDNA detection developed with a Quality by Design approach.

Sci Total Environ 2021 Jun 1;791:148111. Epub 2021 Jun 1.

Department of Chemistry "Ugo Schiff", University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Fi, Italy; "Nanobiosens" Join Lab, University of Florence, Viale Pieraccini 6, 50139 Firenze, Italy. Electronic address:

Environmental DNA (eDNA) is a novel, non-invasive sampling procedure that allows the obtaining of genetic material directly from environmental samples without any evidence of biological sources. The eDNA methodology can greatly benefit from coupling it to reliable, portable and cost-effective tools able to perform decentralized measurements directly at the site of need and in resource-limited settings. Herein, we report a simple method for the selective analysis of eDNA using a magneto-assay with electrochemical detection. Read More

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Diagnostic Performance of a Rapid Immunochromatographic Test Kit for Detecting Canine Parvovirus Infection.

Top Companion Anim Med 2021 Jun 10:100551. Epub 2021 Jun 10.

Department of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria.

In dogs, canine parvovirus (CPV) enteritis is associated with high morbidity and fatality rates requiring early diagnosis to facilitate treatment and reduce its spread. In recent times, various commercial immunochromatographic (IC) test kits are available for rapid diagnosis, which require an assessment of their accuracy. Therefore, precision of a point-of-care IC combination test kit for canine coronavirus (CCoV)/CPV faecal antigen detection was evaluated in this study. Read More

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COVID diagnostics by molecular methods: A systematic review of nucleic acid based testing systems.

Indian J Med Microbiol 2021 Jun 9. Epub 2021 Jun 9.

Department of Clinical Hematology and Director, Tata Medical Center, Kolkata, 700160, India.

Background: The selection of appropriate kit and PCR equipment for the detection of SARS CoV-2 is critically important in view of many options available in the diagnostic market. Since last year many molecular products are available for COVID-19 diagnostics., some of these diagnostics have become commercially available for healthcare workers and clinical laboratories. Read More

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