51,653 results match your criteria pcr assays

Exosome-shuttled miR-7162-3p from human umbilical cord derived mesenchymal stem cells repair endometrial stromal cell injury by restricting APOL6.

Arch Biochem Biophys 2021 Apr 17:108887. Epub 2021 Apr 17.

Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong, 226000, China. Electronic address:

Background: Recent studies have shown that exosomes (Exos) derived from stem cells can be used as paracrine factors to regenerate cells and tissues via shuttling miRNAs. Exos derived from human umbilical cord derived mesenchymal stem cells (UCMSCs) have been found to alleviate mifepristone-induced endometrial stromal cell (ESC) injury in vitro. Information on the functions and mechanisms of Exos from UCMSC-induced endometrial repair is limited and requires more study. Read More

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Detection of Campylobacter jejuni from fresh produce: comparison of culture- and PCR-based techniques, and metagenomic approach for analyses of the microbiome before and after enrichment.

J Food Prot 2021 Apr 20. Epub 2021 Apr 20.

Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.

In this study, we compared the efficiency of culture-based methods with or without membrane filtration, real-time PCR and digital droplet PCR (ddPCR) for the detection of Campylobacter in fresh produce. Alfalfa sprouts, clover sprouts, coleslaw, and lettuce salad spiked with Campylobacter jejuni ( C. jejuni ) were enriched in Bolton broth for 48 h and enrichment cultures were either directly inoculated onto modified charcoal-cefoperazone-deoxycholate agar or applied on membrane filters placed on the surface of plating media. Read More

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Measuring nonhomologous end-joining, homologous recombination and alternative end-joining simultaneously at an endogenous locus in any transfectable human cell.

Nucleic Acids Res 2021 Apr 20. Epub 2021 Apr 20.

Department of Radiation Oncology, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.

Double strand break (DSB) repair primarily occurs through 3 pathways: non-homologous end-joining (NHEJ), alternative end-joining (Alt-EJ), and homologous recombination (HR). Typical methods to measure pathway usage include integrated cassette reporter assays or visualization of DNA damage induced nuclear foci. It is now well understood that repair of Cas9-induced breaks also involves NHEJ, Alt-EJ, and HR pathways, providing a new format to measure pathway usage. Read More

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Overexpression of circRNA SNRK targets miR-103-3p to reduce apoptosis and promote cardiac repair through GSK3β/β-catenin pathway in rats with myocardial infarction.

Cell Death Discov 2021 Apr 19;7(1):84. Epub 2021 Apr 19.

Section of Pacing and Electrophysiology, Division of Cardiology, the First Affiliated Hospital with Nanjing Medical University, Nanjing, China.

Ischemic cardiomyopathy seriously endangers human health leading to a poor prognosis. Acute myocardial infarction (AMI) is the primary etiology, and the pathophysiological process concludes with the death of cardiomyocytes caused by acute and persistent ischemia and hypoxia in the coronary arteries. We identified a circRNA (circSNRK) which was downregulated in rats with myocardial infarction (MI), however, the role it plays in the MI environment is still unclear. Read More

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HLA-DPB1 rs9277535 polymorphism is associated with rheumatoid arthritis risk in a Chinese Han population.

Aging (Albany NY) 2021 Apr 19;13. Epub 2021 Apr 19.

Department of Orthopedics, The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical University, Changzhou 213003, China.

We used a custom-by-design 48-Plex single nucleotide polymorphism scan™ kit to investigate the relationship between susceptibility to rheumatoid arthritis (RA) and HLA-DPB1 rs9277535 polymorphism in 805 RA patients and 1095 healthy controls from the Chinese Han population. Blood plasma levels of HLA-DPB1 were also examined using enzyme-linked immunosorbent assays in 170 RA patients and 170 matched control individuals. Quantitative reverse transcription PCR (qRT-PCR) was used to evaluate relative HLA-DPB1 mRNA levels in these blood samples as well. Read More

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Placentitis and abortion caused by a multidrug resistant strain of Campylobacter fetus subspecies fetus in a sheep in Uruguay.

Rev Argent Microbiol 2021 Apr 16. Epub 2021 Apr 16.

Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Ruta 50 Km 11, Estación Experimental La Estanzuela, Colonia, Uruguay. Electronic address:

Campylobacter fetusfetus (Cff) is a major infectious cause of abortion in sheep worldwide, and an opportunistic human pathogen. Information on Cff as an ovine abortifacient in South America is limited. We describe a case of abortion caused by a multidrug resistant strain of Cff in a sheep in Uruguay. Read More

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MiR-29c downregulates tumor-expressed B7-H3 to mediate the antitumor NK-cell functions in ovarian cancer.

Gynecol Oncol 2021 Apr 17. Epub 2021 Apr 17.

Department of Gynecologic Oncology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China. Electronic address:

Objective: B7-H3 is a member of the B7 family of immune checkpoint molecule. Although B7-H3 has been shown to regulate T cell-mediated peripheral immune response, whether it also correlated with NK cell exhaustion in ovarian cancer remains unclear. The purpose of this study was to explore the mechanism of B7-H3 regulating NK-cell proliferation and function. Read More

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Human Huntington's disease pluripotent stem cell-derived microglia develop normally but are abnormally hyper-reactive and release elevated levels of reactive oxygen species.

J Neuroinflammation 2021 Apr 19;18(1):94. Epub 2021 Apr 19.

Huntington's Disease Centre, Department of Neurodegenerative Disease, UCL Queen Square Institute of Neurology, University College London, WC1N 3BG, London, UK.

Background: Neuroinflammation may contribute to the pathogenesis of Huntington's disease, given evidence of activated microglia and elevated levels of inflammatory molecules in disease gene carriers, even those many years from symptom onset. We have shown previously that monocytes from Huntington's disease patients are hyper-reactive to stimulation in a manner dependent on their autonomous expression of the disease-causing mutant HTT protein. To date, however, whether human microglia are similarly hyper-responsive in a cell-autonomous manner has not been determined. Read More

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Association between biofilm formation gene bla exoU and metallo and extend spectrum beta-lactamase production of multidrug resistance Pseudomonas aeruginosa in clinical samples.

Fattma Abodi Ali

Comb Chem High Throughput Screen 2021 Apr 18. Epub 2021 Apr 18.

Department of Medical microbiology, College of Health Sciences, Hawler Medical University. Iraq.

Background: The presence of biofilm formation exoU gene is significant challenge to infection control management in hospitals and exposure by Pseudomonas aeruginosa may lead to further spread and development of antimicrobial resistance.

Methods: Out of 227 samples 40 clinical isolates of P. aeruginosa were collected from patients attending public hospitals ( Rizgary, Teaching hospital, Laboratory center, Raparin, Nanakaly hospitals)in Erbil city/Iraq over a period during 2018 to march 2019 and fully characterized by standard bacteriological procedures and antimicrobial susceptibility test and ESBL has been carried out by Vitek 2 compact system and. Read More

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Comparison of four PCR and two point of care assays used in the laboratory detection of SARS-CoV-2.

J Virol Methods 2021 Apr 16:114165. Epub 2021 Apr 16.

Military Medical Centre of the Hungarian Defence Forces, H-1134, Budapest, Róbert Károly krt. 44, Hungary; Semmelweis University - Faculty of Medicine, H-1085, Budapest, Üllői út 26., Hungary. Electronic address:

Seeing the global emergence and the lack of a definitive cure for COVID-19, it is essential to find the most sensitive and specific detection method to identify infected patients in a timely manner. Our paper aims to compare the clinical sensitivity of different commercial RT-qPCR (Genesig, 1copy, DNA-Techonolgy and Charité primer-probe sets), isothermal PCR (Ustar Isothermal Amplification-Real Time Fluorescent Assay) and immunochromatographic antigen detection (BOCREDIT COVID-19 Ag) assays developed to use in laboratory diagnosis of COVID-19. A total of 119 nasopharyngeal swab specimens were collected from symptomatic patients. Read More

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Development and validation of real-time PCR assays for the detection of Ehrlichia species and E. chaffeensis in clinical specimens.

J Microbiol Methods 2021 Apr 16:106225. Epub 2021 Apr 16.

Rickettsial Zoonoses Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, United States of America. Electronic address:

Ehrlichiosis, caused by Gram-negative bacteria of the genus Ehrlichia, is considered an emerging infectious disease due to the increasing number of reported cases. Symptoms are non-specific and occur within 1 to 2 weeks following the bite of an infected tick. Confirmatory laboratory diagnostic methods vary in sensitivity and specimen requirements, which can lead to delayed diagnosis. Read More

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SNHG1/miR-186/FUT8 regulates cell migration and invasion in oral squamous cell carcinoma.

Oral Dis 2021 Apr 19. Epub 2021 Apr 19.

Department of Stomatology, The Hospital of Xidian Group, Xi'an 710077, China.

Recently, lncRNAs are associated with the progression and development of various cancers. We aimed to explore the effects of lncRNA SNHG1 on the proliferation, apoptosis, migration and invasion of oral squamous cell carcinoma (OSCC) cells. Quantitative real-time PCR (RT-qPCR) was used for measurement of SNHG1 in OSCC cells. Read More

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Loss of Speckle-Type POZ Protein Promotes Prostate Cancer Cell Migration and Invasion Through Upregulation of MCP-1.

Med Sci Monit 2021 Apr 19;27:e929199. Epub 2021 Apr 19.

Key Laboratory of Longevity and Aging-Related Disease of the Chinese Ministry of Education, Guangxi Medical University, Nanning, Guangxi, China (mainland).

BACKGROUND The goal of this study is to verify that the loss of speckle-type POZ protein (SPOP) promotes the migration and invasion of prostate cancer cells, and that this process is brought about by an increase in MCP-1. MATERIAL AND METHODS SPOP knockout C4-2 cells (C4-2 SPOP-/-) were verified by western blotting. Transwell and wound-healing assays were applied to verify different migration and invasion abilities between the C4-2 SPOP-/- and control cells. Read More

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Attack rates amongst household members of outpatients with confirmed COVID-19 in Bergen, Norway: A case-ascertained study.

Lancet Reg Health Eur 2021 Apr 31;3:100014. Epub 2021 Mar 31.

Department of Clinical Science (NL, BB), University of Bergen, N-5021 Bergen, Norway.

Background: Households studies reflect the natural spread of SARS-CoV-2 in immunologically naive populations with limited preventive measures to control transmission.We hypothesise that seropositivity provides more accurate household attack rates than RT-PCR. Here, we investigated the importance of age in household transmission dynamics. Read More

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Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification.

J Vis Exp 2021 Mar 31(169). Epub 2021 Mar 31.

Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences;

Diagnosis of the ongoing SARS-CoV-2 pandemic is a priority for all countries across the globe. Currently, reverse transcription quantitative PCR (RT-qPCR) is the gold standard for SARS-CoV-2 diagnosis as no permanent solution is available. However effective this technique may be, research has emerged showing its limitations in detection and diagnosis especially when it comes to low abundant targets. Read More

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Evaluation of bat adenoviruses suggests co-evolution and host roosting behaviour as drivers for diversity.

Microb Genom 2021 Apr;7(4)

Metabiota Inc, Nanaimo, British Columbia, Canada.

Adenoviruses (AdVs) are diverse pathogens of humans and animals, with several dozen bat AdVs already identified. Considering that over 100 human AdVs are known, and the huge diversity of bat species, many bat AdVs likely remain undiscovered. To learn more about AdV prevalence, diversity and evolution, we sampled and tested bats in Cameroon using several PCR assays for viral and host DNA. Read More

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Establishment of primary reference measurement procedures and reference materials for variant detection in non-small cell lung cancer.

Anal Methods 2021 Apr 19. Epub 2021 Apr 19.

Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, People's Republic of China.

Circulating tumor DNA (ctDNA)-based mutation detection is promising to change the clinical practice of genotype-directed therapy for cancer. A growing number of non-invasive tests for cancer screening and monitoring that involve the detection of ctDNA have been commercialized. Primary reference measurement procedures (PRMPs) and reference materials (RMs) are urgently needed to assess the non-invasive tests. Read More

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Novel Peptide NT/K-CFY Derived from Kringle Structure of Neurotrypsin Inhibits Neovascularization.

Curr Eye Res 2021 Apr 19:1-8. Epub 2021 Apr 19.

Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

: To assess the anti-neovascularization effect of a novel peptide NT/K-CFY derived from the kringle domain of neurotrypsin.: Cell migration, lumen formation and cell proliferation assays were performed to determine the anti-neovascularization effect of NT/K-CFY in primary human umbilical vein endothelial cells (HUVECs). Chick chorioallantoic membrane (CAM) and oxygen-induced retinopathy (OIR) models were established to assess the anti-angiogenic role of NT/K-CFY . Read More

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Minicircle-based expression of vascular endothelial growth factor (VEGF) in mesenchymal stromal cells (MSC) from diverse human tissues.

J Gene Med 2021 Apr 18:e3342. Epub 2021 Apr 18.

Department of Bioengineering and iBB - Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.

Background: Mesenchymal stromal cells (MSC) have been exploited for the treatment of ischemic diseases given their angiogenic potential. Despite bone marrow (BM) being the most studied tissue source, cells with similar intrinsic properties can be isolated from adipose tissue (AT) and umbilical cord matrix (UCM). This study aims to compare the angiogenic potential of MSC obtained from BM, AT and UCM that were genetically modified with VEGF-encoding minicircle vectors. Read More

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circ_0003204 Regulates Cell Growth, Oxidative Stress, and Inflammation in ox-LDL-Induced Vascular Endothelial Cells via Regulating miR-942-5p/HDAC9 Axis.

Front Cardiovasc Med 2021 1;8:646832. Epub 2021 Apr 1.

Department of Cardiology, The First Affiliated Hospital of University of South China, Hengyang, China.

Atherosclerosis (AS) is a typical inflammatory vascular disease. Many reports corroborated that circular RNAs (circRNAs) is involved in AS progression. However, the potential function and possible mechanism of circ_0003204 in AS progression remain indistinct. Read More

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LINC01977 Promotes Breast Cancer Progression and Chemoresistance to Doxorubicin by Targeting miR-212-3p/GOLM1 Axis.

Front Oncol 2021 31;11:657094. Epub 2021 Mar 31.

Department of Breast Surgery, General Surgery, Qilu Hospital of Shandong University, Jinan, China.

Long non-coding RNAs(lncRNAs) play an important role in cancer initiation and progression. However, hub lncRNAs involved in breast cancer still remain underexplored. In this study, integrated bioinformatics analysis was used to define LINC01977 as a key oncogenic driver in breast cancer. Read More

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Downregulation of hsa_circ_0001836 Induces Pyroptosis Cell Death in Glioma Cells Epigenetically Upregulating NLRP1.

Front Oncol 2021 31;11:622727. Epub 2021 Mar 31.

Department of Neurosurgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Background: It has been shown that circular RNAs (circRNAs) play a vital role in the progression of glioma. Recently, hsa_circ_0001836 was found to be upregulated in glioma tissues, but the role of hsa_circ_0001836 in glioma remains unclear.

Methods: EdU staining and flow cytometry assays were used to measure the viability and death of glioma cells. Read More

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Long non-coding RNA HOTAIR promotes the progression of synovial sarcoma through microRNA-126/stromal cell-derived factor-1 regulation.

Oncol Lett 2021 Jun 6;21(6):444. Epub 2021 Apr 6.

Department of Orthopedics, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China.

The long non-coding RNA (lncRNA) HOTAIR is an oncogene, that has been reported to be aberrantly expressed in multiple types of malignant tumor tissues. However, its expression and association with synovial sarcoma (SS) remains unclear. The present study aimed to elucidate the expression level of HOTAIR in SS tissues and also identify its role. Read More

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Aquaporin 1 knockdown inhibits triple-negative breast cancer cell proliferation and invasion and .

Oncol Lett 2021 Jun 1;21(6):437. Epub 2021 Apr 1.

Department of Breast Surgery, Guangxi Medical University Cancer Hospital, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China.

Aquaporin 1 (AQP1) contributes to the progression of several cancer types, but its potential involvement in triple-negative breast cancer (TNBC) is unclear. The aim of the present study was to examine the role of AQP1 in cell proliferation and invasion in TNBC. Reverse transcription-quantitative PCR analysis and western blotting were used to detect AQP1 expression in different cell lines. Read More

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Activation of GPER by E2 promotes proliferation, invasion and migration of breast cancer cells by regulating the miR-124/CD151 pathway.

Oncol Lett 2021 Jun 31;21(6):432. Epub 2021 Mar 31.

Department of General Surgery, The Frist Affiliated Hospital of Anhui University of Science and Technology, Huainan, Anhui 232007, P.R. China.

Breast cancer is one of the most common malignancies worldwide and is responsible for a high mortality rate. However, the underlying pathological mechanism of breast cancer remains unclear. MicroRNAs (miRNAs/miRs) play critical roles in the progression of breast cancer. Read More

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Evaluation of Cattle for Naturally Colonized Shiga Toxin-Producing Requires Combinatorial Strategies.

Int J Microbiol 2021 1;2021:6673202. Epub 2021 Apr 1.

Department of Animal and Range Sciences, New Mexico State University, Las Cruces, NW, USA.

Shiga toxin-producing (STEC) serogroups O157, O26, O103, O111, O121, O145, and O45 are designated as food adulterants by the U.S. Department of Agriculture-Food Safety and Inspection Service. Read More

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A Pectate Lyase Is Involved in Virulence and Activation of Host Defense Responses.

Front Plant Sci 2021 22;12:651627. Epub 2021 Mar 22.

Guangdong Laboratory of Lingnan Modern Agriculture, Guangzhou, China.

Plant-parasitic nematodes secrete an array of cell-wall-degrading enzymes to overcome the physical barrier formed by the plant cell wall. Here, we describe a novel pectate lyase gene from . Quantitative real-time PCR assay showed that the highest transcriptional expression level of occurred in pre-parasitic second-stage juveniles, and it was still detected during the early parasitic stage. Read More

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SARS-CoV-2 Antigens Expressed in Plants Detect Antibody Responses in COVID-19 Patients.

Front Plant Sci 2021 31;12:589940. Epub 2021 Mar 31.

Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa.

: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has swept the world and poses a significant global threat to lives and livelihoods, with 115 million confirmed cases and at least 2.5 million deaths from Coronavirus disease 2019 (COVID-19) in the first year of the pandemic. Developing tools to measure seroprevalence and understand protective immunity to SARS-CoV-2 is a priority. Read More

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Silencing PRSS1 suppresses the growth and proliferation of gastric carcinoma cells via the ERK pathway.

Int J Biol Sci 2021 1;17(4):957-971. Epub 2021 Mar 1.

Cancer Research Institute of Hengyang Medical College, University of South China; Key Laboratory of Cancer Cellular and Molecular Pathology in Hunan Province, Hunan Hengyang 421001, China.

Gastric carcinoma (GC) is one of the most common malignant tumors and seriously threatens human life and health. In the present study, 243 differentially expressed proteins in GC were identified using laser capture microdissection (LCM) combined with isotopically labeled quantitative proteomics technology. The expression of serine protease 1 (PRSS1) protein was analyzed by immunohistochemistry and Western blot. Read More

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MicroRNA-532-5p protects against cerebral ischemia-reperfusion injury by directly targeting CXCL1.

Aging (Albany NY) 2021 Apr 18;13. Epub 2021 Apr 18.

Department of Central Laboratory, The Yancheng School of Clinical Medicine of Nanjing Medical University, Yancheng 224001, Jiangsu, China.

We investigated the function of microRNA (miR)-532-5p in cerebral ischemia-reperfusion injury (CI/RI) and the underlying mechanisms using oxygen-glucose deprivation and reperfusion (OGD/R)-treated SH-SY5Y cells and middle cerebral artery occlusion (MCAO) model rats. MiR-532-5p levels were significantly downregulated in OGD/R-treated SH-SY5Y cells and the brain tissues of MCAO model rats. MiR-532-5p overexpression significantly reduced apoptosis, reactive oxygen species (ROS), and inflammation in the OGD/R-induced SH-SY5Y cells. Read More

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