53 results match your criteria pcg ring1

The Polycomb group protein Ring1 regulates dorsoventral patterning of the mouse telencephalon.

Nat Commun 2020 11 11;11(1):5709. Epub 2020 Nov 11.

Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.

Dorsal-ventral patterning of the mammalian telencephalon is fundamental to the formation of distinct functional regions including the neocortex and ganglionic eminence. While Bone morphogenetic protein (BMP), Wnt, and Sonic hedgehog (Shh) signaling are known to determine regional identity along the dorsoventral axis, how the region-specific expression of these morphogens is established remains unclear. Here we show that the Polycomb group (PcG) protein Ring1 contributes to the ventralization of the mouse telencephalon. Read More

View Article and Full-Text PDF
November 2020

Evolution and conservation of polycomb repressive complex 1 core components and putative associated factors in the green lineage.

BMC Genomics 2019 Jun 28;20(1):533. Epub 2019 Jun 28.

Key Laboratory of Crop Epigenetic Regulation and Development in Hunan Province, Hunan Agricultural University, Changsha, 410128, China.

Background: Polycomb group (PcG) proteins play important roles in animal and plant development and stress response. Polycomb repressive complex 1 (PRC1) and PRC2 are the key epigenetic regulators of gene expression, and are involved in almost all developmental stages. PRC1 catalyzes H2A monoubiquitination resulting in transcriptional silencing or activation. Read More

View Article and Full-Text PDF

Variant PRC1 competes with retinoic acid-related signals to repress in the mouse distal forelimb bud.

Development 2018 10 11;145(19). Epub 2018 Oct 11.

Laboratory for Developmental Genetics, RIKEN Center for Integrative Medical Sciences (RIKEN-IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan

Suppression of Meis genes in the distal limb bud is required for proximal-distal (PD) specification of the forelimb. Polycomb group (PcG) factors play a role in downregulation of retinoic acid (RA)-related signals in the distal forelimb bud, causing Meis repression. It is, however, not known whether downregulation of RA-related signals and PcG-mediated proximal gene repression are functionally linked. Read More

View Article and Full-Text PDF
October 2018

Expression and clinical significance of PcG-associated protein RYBP in hepatocellular carcinoma.

Oncol Lett 2017 Jan 11;13(1):141-150. Epub 2016 Nov 11.

Department of Epidemiology and Statistics, School of Public Health, Guilin Medical University, Guilin, Guangxi 541000, P.R. China; Department of Hepatobiliary Surgery, The Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541000, P.R. China.

Ring1 and YY1 binding protein (RYBP), a member of the polycomb group proteins, has been implicated in transcription repression and tumor cell-specific apoptosis. Previously, RYBP has been reported as a putative tumor suppressor in cancer tissues by regulating mouse double minute 2 homolog-p53 signaling. However, the exact role and underlying mechanisms of RYBP in cancer remain to be fully elucidated. Read More

View Article and Full-Text PDF
January 2017

Spatiotemporal Patterns of RING1 Expression after Rat Spinal Cord Injury.

Neurochem Res 2017 Apr 28;42(4):1191-1201. Epub 2016 Dec 28.

Key Laboratory of Nerve Regeneration, Nantong University, Nantong, 226001, Jiangsu, China.

Ring finger protein 1 (RING1) is a RING domain characterized protein belonging to the RING finger family. It is an E3 ubiquitin-protein ligase that mediated monoubiquitination of histone H2A and the core component of PRC1 complex, which is the repressive multiprotein complex of Polycomb group (PcG). Previous studies showed the important tumorigenic role of RING1 via promoting cell proliferation and the crucial function in maintaining transcriptional program stability during development. Read More

View Article and Full-Text PDF

The evolutionary landscape of PRC1 core components in green lineage.

Planta 2016 Apr 4;243(4):825-46. Epub 2016 Jan 4.

College of Bioscience and Biotechnology, International Associated Laboratory of CNRS-Fudan-HUNAU on Plant Epigenome Research, Hunan Agricultural University, 410128, Changsha, China.

Main Conclusion: The origin and evolution of plant PRC1 core components. Polycomb repressive complex1 (PRC1) plays critical roles in epigenetic silencing of homeotic genes and determination of cell fate. Animal PRC1 has been well investigated for a long time, whereas plant PRC1 was just confirmed in recent years. Read More

View Article and Full-Text PDF

RING1 proteins contribute to early proximal-distal specification of the forelimb bud by restricting Meis2 expression.

Development 2016 Jan 16;143(2):276-85. Epub 2015 Dec 16.

Laboratory for Developmental Genetics, RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan CREST, Japan Science and Technology Agency, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan

Polycomb group (PcG) proteins play a pivotal role in silencing developmental genes and help to maintain various stem and precursor cells and regulate their differentiation. PcG factors also regulate dynamic and complex regional specification, particularly in mammals, but this activity is mechanistically not well understood. In this study, we focused on proximal-distal (PD) patterning of the mouse forelimb bud to elucidate how PcG factors contribute to a regional specification process that depends on developmental signals. Read More

View Article and Full-Text PDF
January 2016

RYBP predicts survival of patients with non-small cell lung cancer and regulates tumor cell growth and the response to chemotherapy.

Cancer Lett 2015 Dec 21;369(2):386-95. Epub 2015 Sep 21.

Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX 79106, USA; Cancer Biology Center, Texas Tech University Health Sciences Center, Amarillo, TX 79106, USA. Electronic address:

Ring1 and YY1 binding protein (RYBP) is a member of the Polycomb group (PcG) proteins and regulates cell growth through both PcG-dependent and -independent mechanisms. Our initial study indicated that RYBP is down-regulated in human non-small cell lung cancer (NSCLC) tissues. The present study determined the molecular role of RYBP in the development of NSCLC. Read More

View Article and Full-Text PDF
December 2015

PRC1 components exhibit different binding kinetics in Polycomb bodies.

Biol Cell 2014 Apr 6;106(4):111-25. Epub 2014 Mar 6.

Biophotonique Cellulaire Fonctionnelle, Interdisciplinary Research Institute, Université Lille 1 - CNRS USR 3078, Parc de la Haute Borne, Villeneuve d'Ascq, 59658, France.

Background Information: Polycomb group (PcG) proteins keep the memory of cell identity by maintaining the repression of numerous target genes. They accumulate into nuclear foci called Polycomb bodies, which function in Drosophila cells as silencing compartments where PcG target genes convene. PcG proteins also exert their activities elsewhere in the nucleoplasm. Read More

View Article and Full-Text PDF

Polycomb potentiates meis2 activation in midbrain by mediating interaction of the promoter with a tissue-specific enhancer.

Dev Cell 2014 Jan 26;28(1):94-101. Epub 2013 Dec 26.

Developmental Genetics Laboratory, RIKEN-IMS, Japan Science and Technology Agency, 1-7-22 Suehirocho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan; CREST, Japan Science and Technology Agency, 1-7-22 Suehirocho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan. Electronic address:

Polycomb-group (PcG) proteins mediate repression of developmental regulators in a reversible manner, contributing to their spatiotemporally regulated expression. However, it is poorly understood how PcG-repressed genes are activated by developmental cues. Here, we used the mouse Meis2 gene as a model to identify a role of a tissue-specific enhancer in removing PcG from the promoter. Read More

View Article and Full-Text PDF
January 2014

The isolated N terminus of Ring1B is a well-folded, monomeric fragment with native-like structure.

Protein Eng Des Sel 2014 Jan 26;27(1):1-11. Epub 2013 Nov 26.

Departamento de Química-Física, Bioquímica y Química Inorgánica, Agrifood Campus of International Excellence (ceiA3), Universidad de Almería, 04120 Almería, Spain.

The Polycomb group (PcG) proteins assemble into Polycomb repressive complexes (PRCs), PRC1 and PRC2, which act as general transcriptional repressors. PRC1 comprises a variety of biochemical entities endowed with histone H2A monoubiquitylation activity conferred by really interesting new gene (RING) finger E3 ubiquitin ligases Ring1A and Ring1B. All PRC1 complexes contain Ring1 proteins which are essential for Polycomb epigenetic regulation. Read More

View Article and Full-Text PDF
January 2014

Dynamics of Polycomb chromatin domains under conditions of increased molecular crowding.

Biol Cell 2013 Nov 16;105(11):519-34. Epub 2013 Sep 16.

Charles University in Prague, First Faculty of Medicine, Institute of Cellular Biology and Pathology, Czech Republic.

Background Information: A Polycomb (PcG) body is an orphan nuclear subcompartment characterised by accumulations of Polycomb repressive complex 1 (PRC1) proteins. However, seemingly contradictory reports have appeared that describe the PcG bodies either as protein-based bodies in the interchromatin compartment or chromatin domains. In this respect, molecular crowding is an important factor for the assembly and stability of nuclear subcompartments. Read More

View Article and Full-Text PDF
November 2013

L3MBTL2 protein acts in concert with PcG protein-mediated monoubiquitination of H2A to establish a repressive chromatin structure.

Mol Cell 2011 May;42(4):438-50

Howard Hughes Medical Institute, NYU Medical School, 522 First Avenue, New York, NY 10016, USA.

We have identified human MBT domain-containing protein L3MBTL2 as an integral component of a protein complex that we termed Polycomb repressive complex 1 (PRC1)-like 4 (PRC1L4), given the copresence of PcG proteins RING1, RING2, and PCGF6/MBLR. PRC1L4 also contained E2F6 and CBX3/HP1γ, known to function in transcriptional repression. PRC1L4-mediated repression necessitated L3MBTL2 that compacted chromatin in a histone modification-independent manner. Read More

View Article and Full-Text PDF

Association of Rex-1 to target genes supports its interaction with Polycomb function.

Stem Cell Res 2011 Jul 2;7(1):1-16. Epub 2011 Mar 2.

Regenerative Medicine Programme, IIS Aragón, Instituto Aragonés de Ciencias de la Salud, Zaragoza, Avda. Gómez Laguna, 25, Pl. 11, 50009 Zaragoza, Spain.

Rex-1/Zfp42 displays a remarkably restricted pattern of expression in preimplantation embryos, primary spermatocytes, and undifferentiated mouse embryonic stem (ES) cells and is frequently used as a marker gene for pluripotent stem cells. To understand the role of Rex-1 in selfrenewal and pluripotency, we used Rex-1 association as a measure to identify potential target genes, and carried out chromatin-immunoprecipitation assays in combination with gene specific primers to identify genomic targets Rex-1 associates with. We find association of Rex-1 to several genes described previously as bivalently marked regulators of differentiation and development, whose repression in mouse embryonic stem (ES) cells is Polycomb Group-mediated, and controlled directly by Ring1A/B. Read More

View Article and Full-Text PDF

Interaction proteomics analysis of polycomb proteins defines distinct PRC1 complexes in mammalian cells.

Mol Cell Proteomics 2011 Apr 31;10(4):M110.002642. Epub 2011 Jan 31.

Chromatinomics, Interdisciplinary Research Institute, Univ. Lille Nord de France, Université de Lille 1 Sciences et Technologies/CNRS USR 3078, 50 Avenue Halley, Parc Scientifique de la Haute Borne, F-59658 Villeneuve d'Ascq Cedex, France.

Polycomb group (PcG) proteins maintain transcriptional repression of hundreds of genes involved in development, signaling or cancer using chromatin-based epigenetic mechanisms. Biochemical studies in Drosophila have revealed that PcG proteins associate in at least two classes of protein complexes known as Polycomb repressive complexes 1 and 2 (PRC1 and PRC2). Drosophila core PRC1 is composed of four subunits, Polycomb (Pc), Sex combs extra (Sce), Polyhomeotic (Ph), and Posterior sex combs (Psc). Read More

View Article and Full-Text PDF

Overexpression of the EZH2, RING1 and BMI1 genes is common in myelodysplastic syndromes: relation to adverse epigenetic alteration and poor prognostic scoring.

Ann Hematol 2011 Jun 2;90(6):643-53. Epub 2010 Dec 2.

Department of Hematology, The Sixth people Hospital, Shanghai JiaoTong University, ShangHai Jiaotong University School of Medicine, Shanghai, 200233, People's Republic of China.

Epigenetics refers to the study of clonally inherited changes in gene expression without accompanying genetic changes. Previous research on the epigenetics of myelodysplastic syndromes (MDS) mainly focused on the inactivation of tumor suppressor genes as a result of DNA methylation. However, the basic molecular pathogenesis of epigenetics in MDS remains poorly understood. Read More

View Article and Full-Text PDF

BMI1-mediated histone ubiquitylation promotes DNA double-strand break repair.

J Cell Biol 2010 Oct;191(1):45-60

Department of Oncology, University of Alberta, Edmonton, T6G 1Z2 Alberta, Canada.

Polycomb group (PcG) proteins are major determinants of cell identity, stem cell pluripotency, and epigenetic gene silencing during development. The polycomb repressive complex 1, which contains BMI1, RING1, and RING2, functions as an E3-ubuiquitin ligase. We found that BMI1 and RING2 are recruited to sites of DNA double-strand breaks (DSBs) where they contribute to the ubiquitylation of γ-H2AX. Read More

View Article and Full-Text PDF
October 2010

Role of polycomb proteins Ring1A and Ring1B in the epigenetic regulation of gene expression.

Miguel Vidal

Int J Dev Biol 2009 ;53(2-3):355-70

Department of Developmental and Cell Biology, Centro de Investigaciones Biologicas, CSIC, Madrid, Spain.

Generation of cell diversity depends on epigenetic regulatory mechanisms. Polycomb group (PcG) proteins are central components of epigenetic regulation in metazoans. The system, initially associated with transcriptional program stability during development, is also involved in the regulation of other processes, such as maintenance of stem cell pluripotency and cell proliferation. Read More

View Article and Full-Text PDF
September 2009

RYBP stabilizes p53 by modulating MDM2.

EMBO Rep 2009 Feb 19;10(2):166-72. Epub 2008 Dec 19.

Department of Pharmacology and Toxicology, University of Alabama at Birmingham, 1670 University Boulevard, Volker Hall 113, Birmingham, Alabama 35294, USA.

The mouse double minute 2 (MDM2)-p53 interaction regulates the activity of p53 and is a potential target for human cancer therapy. Here, we report that RYBP (RING1- and YY1-binding protein), a member of the polycomb group (PcG), interacts with MDM2 and decreases MDM2-mediated p53 ubiquitination, leading to stabilization of p53 and an increase in p53 activity. RYBP induces cell-cycle arrest and is involved in the p53 response to DNA damage. Read More

View Article and Full-Text PDF
February 2009

Expression parameters of the polycomb group proteins BMI1, SUZ12, RING1 and CBX7 in urothelial carcinoma of the bladder and their prognostic relevance.

Tumour Biol 2008 5;29(5):323-9. Epub 2008 Nov 5.

Department of Urology, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Background: The polycomb group (PCG) proteins are epigenetic transcriptional repressors involved in the control of cellular proliferation and oncogenesis. This study aimed at examining whether mRNA tumor levels of the PCG family members BMI1, SUZ12, RING1, and CBX7 relate to histopathological parameters in urothelial carcinomas of the bladder and whether they may provide prognostic information following tumor resection.

Methods: The relative gene expression of BMI1, SUZ12, RING1, and CBX7 was analyzed by real-time RT-PCR in tumor tissue obtained from 93 patients with urothelial carcinoma of the bladder undergoing surgical treatment. Read More

View Article and Full-Text PDF
January 2009

Structural transitions of the RING1B C-terminal region upon binding the polycomb cbox domain.

Biochemistry 2008 Aug 11;47(31):8007-15. Epub 2008 Jul 11.

Department of Biochemistry, University of Texas Health Science Center at San Antonio, MSC 7760, 7703 Floyd Curl Drive, San Antonio, Texas 78229-3900, USA.

Polycomb group (PcG) proteins are required for maintaining cell identity and stem cell self-renewal. RING1B and Polycomb (Pc) are two components of a multiprotein complex called polycomb repression complex 1 (PRC1) that is essential for establishing and maintaining long-term repressed gene states. Here we characterize the interaction between the C-terminal region of RING1B (C-RING1B) and the Pc cbox domain. Read More

View Article and Full-Text PDF

Polycomb group proteins Ring1A/B are functionally linked to the core transcriptional regulatory circuitry to maintain ES cell identity.

Development 2008 Apr 13;135(8):1513-24. Epub 2008 Mar 13.

RIKEN Research Center for Allergy and Immunology, 1-7-22 Suehiro, Tsurumi-ku, Yokohama 230-0045, Japan.

The Polycomb group (PcG) proteins mediate heritable silencing of developmental regulators in metazoans, participating in one of two distinct multimeric protein complexes, the Polycomb repressive complexes 1 (PRC1) and 2 (PRC2). Although PRC2 has been shown to share target genes with the core transcription network, including Oct3/4, to maintain embryonic stem (ES) cells, it is still unclear whether PcG proteins and the core transcription network are functionally linked. Here, we identify an essential role for the core PRC1 components Ring1A/B in repressing developmental regulators in mouse ES cells and, thereby, in maintaining ES cell identity. Read More

View Article and Full-Text PDF

Polycomb-group oncogenes EZH2, BMI1, and RING1 are overexpressed in prostate cancer with adverse pathologic and clinical features.

Eur Urol 2007 Aug 17;52(2):455-63. Epub 2006 Nov 17.

Department of Pathology, Erasmus Medical Center, Rotterdam, The Netherlands.

Objectives: Polycomb group (PcG) proteins are involved in maintenance of cell identity and proliferation. The protein EZH2 is overexpressed in disseminated prostate cancer, implicating a role of PcG complexes in tumor progression. In this study, we evaluated the expression of eight members of both PcG complexes in clinicopathologically defined prostate cancer. Read More

View Article and Full-Text PDF

Regulation of Th2 cell development by Polycomb group gene bmi-1 through the stabilization of GATA3.

J Immunol 2006 Dec;177(11):7656-64

Department of Immunology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.

The Polycomb group (PcG) gene products regulate the maintenance of the homeobox gene expression in Drosophila and vertebrates and also the cell cycle progression in thymocytes and Th2 cell differentiation in mature T cells. We herein studied the role of PcG gene bmi-1 product in Th1/Th2 cell differentiation and found that Bmi-1 facilitates Th2 cell differentiation in a Ring finger-dependent manner. Biochemical studies indicate that Bmi-1 interacts with GATA3 in T cells, which is dependent on the Ring finger of Bmi-1. Read More

View Article and Full-Text PDF
December 2006

Polycomb group and SCF ubiquitin ligases are found in a novel BCOR complex that is recruited to BCL6 targets.

Mol Cell Biol 2006 Sep;26(18):6880-9

Department of Genetics, Cell Biology and Development, 6-160 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455, USA.

The corepressor BCOR potentiates transcriptional repression by the proto-oncoprotein BCL6 and suppresses the transcriptional activity of a common mixed-lineage leukemia fusion partner, AF9. Mutations in human BCOR cause male lethal, X-linked oculofaciocardiodental syndrome. We identified a BCOR complex containing Polycomb group (PcG) and Skp-Cullin-F-box subcomplexes. Read More

View Article and Full-Text PDF
September 2006

Homeotic transformations of the axial skeleton of YY1 mutant mice and genetic interaction with the Polycomb group gene Ring1/Ring1A.

Mech Dev 2006 Apr 18;123(4):312-20. Epub 2006 Apr 18.

Developmental and Cell Biology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.

Polycomb group (PcG) proteins participate in the maintenance of transcriptionally repressed state of genes relevant to cell differentiation. Here, we show anterior homeotic transformations of the axial skeleton of YY1(+/-) mice. We find that the penetrance of some of these alterations was reduced in mice that are deficient in the class II PcG gene Ring1/Ring1A, indicating a genetic interaction between those two genes. Read More

View Article and Full-Text PDF

Variability in the expression of polycomb proteins in different normal and tumoral tissues. A pilot study using tissue microarrays.

Mod Pathol 2006 May;19(5):684-94

Lymphoma Group, Molecular Pathology Programme, Centro Nacional de Investigaciones Oncológicas (CNIO), Madrid, Spain.

In spite of the known function of polycomb group (PcG) genes in stem cell self-renewal, control of cellular proliferation and differentiation, its role in cancer pathogenesis is still poorly understood. We studied the expression by immunohistochemistry of several PcG-maintenance complex proteins (RING1, RNF2, BMI1, MEL18, HPH1 and RYBP) in nontumoral (154 samples) and tumoral (550 samples) human tissues using Tissue Microarrays. For selected genes (BMI1 and RING1) FISH analysis has been also carried out. Read More

View Article and Full-Text PDF

Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing.

Mol Cell 2005 Dec;20(6):845-54

Howard Hughes Medical Institute, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 27599, USA.

Polycomb group (PcG) proteins exist in at least two biochemically distinct protein complexes, the EED-EZH2 complex and the PRC1 complex, that respectively possess H3-K27 methyltransferase and H2A-K119 ubiquitin E3 ligase activities. How the enzymatic activities are regulated and what their role is in Hox gene silencing are not clear. Here, we demonstrate that Bmi-1 and Ring1A, two components of the PRC1 complex, play important roles in H2A ubiquitylation and Hox gene silencing. Read More

View Article and Full-Text PDF
December 2005

Abnormal PcG protein expression in Hodgkin's lymphoma. Relation with E2F6 and NFkappaB transcription factors.

J Pathol 2004 Dec;204(5):528-37

Molecular Pathology Programme, Centro Nacional de Investigaciones Oncológicas (CNIO), C/Melchor Fernández Almagro 3, E-28029 Madrid, Spain.

The Polycomb group (PcG) of proteins comprises a family of repressors of homeobox genes that play key roles in body formation, haematopoiesis and cell cycle control. In this study, a large-scale analysis of PcG protein expression (BMI1, MEL18, PH1, RNF2, RING1, and RYBP) was performed in 321 Hodgkin's lymphoma (HL) biopsies and in reactive lymphoid tissues using tissue microarrays. The relevance of PcG proteins in HL was also investigated by the simultaneous analysis of PcG and other proteins involved in the control of cell cycle, transcription machinery and lymphoid differentiation. Read More

View Article and Full-Text PDF
December 2004

The Drosophila Polycomb group gene Sex combs extra encodes the ortholog of mammalian Ring1 proteins.

Mech Dev 2004 May;121(5):449-62

Centro de Biologia Molecular Severo Ochoa, CSIC-UAM Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain.

In Drosophila, the Polycomb group (PcG) of genes is required for the maintenance of homeotic gene repression during development. Here, we have characterized the Drosophila ortholog of the products of the mammalian Ring1/Ring1A and Rnf2/Ring1B genes. We show that Drosophila Ring corresponds to the Sex combs extra (Sce), a previously described PcG gene. Read More

View Article and Full-Text PDF