485 results match your criteria patterning transgene

A transgenic Alx4-CreER mouse to analyze anterior limb and nephric duct development.

Dev Dyn 2021 Mar 16. Epub 2021 Mar 16.

Department of Cell, Development, and Integrative Biology, University of Alabama at Birmingham, Birmingham, Alabama, USA.

Background: Genetic tools to study gene function and the fate of cells in the anterior limb bud are very limited.

Results: We describe a transgenic mouse line expressing CreER from the Aristaless-like 4 (Alx4) promoter that induces recombination in the anterior limb. Cre induction at embryonic day 8. Read More

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Transient Nodal Signaling in Left Precursors Coordinates Opposed Asymmetries Shaping the Heart Loop.

Dev Cell 2020 11 9;55(4):413-431.e6. Epub 2020 Nov 9.

Université de Paris, Imagine - Institut Pasteur, Unit of Heart Morphogenesis, INSERM UMR1163, 75015 Paris, France. Electronic address:

The secreted factor Nodal, known as a major left determinant, is associated with severe heart defects. Yet, it has been unclear how it regulates asymmetric morphogenesis such as heart looping, which align cardiac chambers to establish the double blood circulation. Here, we report that Nodal is transiently active in precursors of the mouse heart tube poles, before looping. Read More

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November 2020

Thermofluidic heat exchangers for actuation of transcription in artificial tissues.

Sci Adv 2020 Sep 30;6(40). Epub 2020 Sep 30.

Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.

Spatial patterns of gene expression in living organisms orchestrate cell decisions in development, homeostasis, and disease. However, most methods for reconstructing gene patterning in 3D cell culture and artificial tissues are restricted by patterning depth and scale. We introduce a depth- and scale-flexible method to direct volumetric gene expression patterning in 3D artificial tissues, which we call "heat exchangers for actuation of transcription" (HEAT). Read More

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September 2020

Mutations in Ciliary Trafficking Genes affect Sonic Hedgehog-dependent Neural Tube Patterning Differentially along the Anterior-Posterior Axis.

Neuroscience 2020 12 16;450:3-14. Epub 2020 Jul 16.

Vertebrate Developmental Biology Program, Department of Pediatrics, Yale School of Medicine, 333 Cedar Street, New Haven, CT 06520, United States. Electronic address:

Cell specification in the ventral spinal cord is a well-studied model system to understand how tissue pattern develops in response to a morphogen gradient. Ventral cell types including motor neurons (MNs) are induced in the neural tube in response to graded Sonic Hedgehog (Shh) signaling. We performed a forward genetic screen in the mouse that incorporated a GFP-expressing transgene to visualize MNs to identify genes regulating ventral patterning. Read More

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December 2020

Segregation of an MSH1 RNAi transgene produces heritable non-genetic memory in association with methylome reprogramming.

Nat Commun 2020 05 5;11(1):2214. Epub 2020 May 5.

Departments of Biology and Plant Science, The Pennsylvania State University, University Park, PA, USA.

MSH1 is a plant-specific protein. RNAi suppression of MSH1 results in phenotype variability for developmental and stress response pathways. Segregation of the RNAi transgene produces non-genetic msh1 'memory' with multi-generational inheritance. Read More

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Transcriptional Analyses of Innate and Acquired Immune Patterning Elicited by Marek's Disease Virus Vaccine Strains: Turkey Herpesvirus (HVT), Marek's Disease Virus 2 (strain SB1), and Bivalent Vaccines (HVT/SB1 and HVT-LT/SB1).

Avian Dis 2019 12;63(4):670-680

Department of Animal and Food Sciences, University of Delaware, Newark, DE 19716,

Marek's disease (MD) is a complex pathology of chickens caused by MD virus (MDV) 1 and is observed as paralysis, immune suppression, neurologic signs, and the rapid formation of T-cell lymphomas. The incidence of MD in commercial broilers is largely controlled via vaccination, either or at hatch with live attenuated vaccines, i.e. Read More

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December 2019

The minimal gap-junction network among melanophores and xanthophores required for stripe pattern formation in zebrafish.

Development 2019 11 15;146(22). Epub 2019 Nov 15.

Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan

Connexin 39.4 (Cx39.4) and connexin 41. Read More

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November 2019

Disruption of the pancreatic vasculature in zebrafish affects islet architecture and function.

Development 2019 11 4;146(21). Epub 2019 Nov 4.

Department of Developmental Genetics, Max Planck Institute for Heart and Lung Research, 61231 Bad Nauheim, Germany

A dense local vascular network is crucial for pancreatic endocrine cells to sense metabolites and secrete hormones, and understanding the interactions between the vasculature and the islets may allow for therapeutic modulation in disease conditions. Using live imaging in two models of vascular disruption in zebrafish, we identified two distinct roles for the pancreatic vasculature. At larval stages, expression of a dominant negative version of Vegfaa (dnVegfaa) in β-cells led to vascular and endocrine cell disruption with a minor impairment in β-cell function. Read More

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November 2019

Asymmetric histone inheritance via strand-specific incorporation and biased replication fork movement.

Nat Struct Mol Biol 2019 08 29;26(8):732-743. Epub 2019 Jul 29.

Department of Biology, The Johns Hopkins University, Baltimore, MD, USA.

Many stem cells undergo asymmetric division to produce a self-renewing stem cell and a differentiating daughter cell. Here we show that, similarly to H3, histone H4 is inherited asymmetrically in Drosophila melanogaster male germline stem cells undergoing asymmetric division. In contrast, both H2A and H2B are inherited symmetrically. Read More

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Growth differentiation factor 11 locally controls anterior-posterior patterning of the axial skeleton.

J Cell Physiol 2019 12 10;234(12):23360-23368. Epub 2019 Jun 10.

Department of Molecular Genetics and Dental Pharmacology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea.

Growth and differentiation factor 11 (GDF11) is a transforming growth factor β family member that has been identified as the central player of anterior-posterior (A-P) axial skeletal patterning. Mice homozygous for Gdf11 deletion exhibit severe anterior homeotic transformations of the vertebrae and craniofacial defects. During early embryogenesis, Gdf11 is expressed predominantly in the primitive streak and tail bud regions, where new mesodermal cells arise. Read More

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December 2019

An FDA-Approved Drug Screen for Compounds Influencing Craniofacial Skeletal Development and Craniosynostosis.

Mol Syndromol 2019 02 21;10(1-2):98-114. Epub 2018 Jul 21.

Genetics and Genomic Medicine, UCL Institute of Child Health, London, UK.

Neural crest stem/progenitor cells (NCSCs) populate a variety of tissues, and their dysregulation is implicated in several human diseases including craniosynostosis and neuroblastoma. We hypothesised that small molecules that inhibit NCSC induction or differentiation may represent potential therapeutically relevant drugs in these disorders. We screened 640 FDA-approved compounds currently in clinical use for other conditions to identify those which disrupt development of NCSC-derived skeletal elements that form the zebrafish jaw. Read More

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February 2019

Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos.

Clare E Buckley

Methods Mol Biol 2019 ;1920:143-162

Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK.

This chapter describes the use of optogenetic heterodimerization in single cells within whole-vertebrate embryos. This method allows the use of light to reversibly bind together an "anchor" protein and a "bait" protein. Proteins can therefore be directed to specific subcellular compartments, altering biological processes such as cell polarity and signaling. Read More

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Modeling craniofacial development reveals spatiotemporal constraints on robust patterning of the mandibular arch.

PLoS Comput Biol 2018 11 27;14(11):e1006569. Epub 2018 Nov 27.

Center for Complex Biological Systems, University of California, Irvine, CA, United States of America.

How does pattern formation occur accurately when confronted with tissue growth and stochastic fluctuations (noise) in gene expression? Dorso-ventral (D-V) patterning of the mandibular arch specifies upper versus lower jaw skeletal elements through a combination of Bone morphogenetic protein (Bmp), Endothelin-1 (Edn1), and Notch signaling, and this system is highly robust. We combine NanoString experiments of early D-V gene expression with live imaging of arch development in zebrafish to construct a computational model of the D-V mandibular patterning network. The model recapitulates published genetic perturbations in arch development. Read More

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November 2018

Left/right asymmetric collective migration of parapineal cells is mediated by focal FGF signaling activity in leading cells.

Proc Natl Acad Sci U S A 2018 10 3;115(42):E9812-E9821. Epub 2018 Oct 3.

Department of Cell and Developmental Biology, University College London, WC1E 6BT London, United Kingdom.

The ability of cells to collectively interpret surrounding environmental signals underpins their capacity to coordinate their migration in various contexts, including embryonic development and cancer metastasis. One tractable model for studying collective migration is the parapineal, a left-sided group of neurons that arises from bilaterally positioned precursors that undergo a collective migration to the left side of the brain. In zebrafish, the migration of these cells requires Fgf8 and, in this study, we resolve how FGF signaling correlates with-and impacts the migratory dynamics of-the parapineal cell collective. Read More

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October 2018

Investigating the mechanistic basis of biomechanical input controlling skeletal development: exploring the interplay with Wnt signalling at the joint.

Philos Trans R Soc Lond B Biol Sci 2018 09 24;373(1759). Epub 2018 Sep 24.

Department of Zoology, School of Natural Sciences, Trinity College Dublin, The University of Dublin, Dublin, Ireland

Embryo movement is essential to the formation of a functional skeleton. Using mouse and chick models, we previously showed that mechanical forces influence gene regulation and tissue patterning, particularly at developing limb joints. However, the molecular mechanisms that underpin the influence of mechanical signals are poorly understood. Read More

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September 2018

Quantitative Analysis of Intestinal Stem Cell Dynamics Using Microfabricated Cell Culture Arrays.

Methods Mol Biol 2018 ;1842:139-166

Department of Medicine, Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Regeneration of intestinal epithelium is fueled by a heterogeneous population of rapidly proliferating stem cells (ISCs) found in the base of the small intestine and colonic crypts. ISCs populations can be enriched by fluorescence-activated cell sorting (FACS) based on expression of combinatorial cell surface markers, and fluorescent transgenes. Conventional ISC culture is performed by embedding single ISCs or whole crypt units in a matrix and culturing in conditions that stimulate or repress key pathways to recapitulate ISC niche signaling. Read More

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Cre/lox-controlled spatiotemporal perturbation of FGF signaling in zebrafish.

Dev Dyn 2018 10;247(10):1146-1159

Institute of Molecular Life Sciences, University of Zürich, Zürich, Switzerland.

Background: Spatiotemporal perturbation of signaling pathways in vivo remains challenging and requires precise transgenic control of signaling effectors. Fibroblast growth factor (FGF) signaling guides multiple developmental processes, including body axis formation and cell fate patterning. In zebrafish, mutants and chemical perturbations affecting FGF signaling have uncovered key developmental processes; however, these approaches cause embryo-wide perturbations, rendering assessment of cell-autonomous vs. Read More

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October 2018

Characterization of a morphogenetic furrow specific Gal4 driver in the developing Drosophila eye.

PLoS One 2018 27;13(4):e0196365. Epub 2018 Apr 27.

Department of Biology, University of Dayton, Dayton, OH, United States of America.

The ability to express a gene of interest in a spatio-temporal manner using Gal4-UAS system has allowed the use of Drosophila model to study various biological phenomenon. During Drosophila eye development, a synchronous wave of differentiation called Morphogenetic furrow (MF) initiates at the posterior margin resulting in differentiation of retinal neurons. This synchronous differentiation is also observed in the differentiating retina of vertebrates. Read More

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Adv Exp Med Biol 2018 ;1029:1-3

Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, Japan.

The chordate ascidians, the major group of tunicate s, is the best animal group for studying molecular and cellular processes underlying formation of a chordate body plan. For these studies, transgenic technologies are powerful. Transgenesis of ascidians has a long history of more than 20 years, and many practical tips have been accumulated. Read More

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November 2018

The cluster represses receptor tyrosine kinase signaling to determine cell fates in the eye.

Development 2018 04 9;145(7). Epub 2018 Apr 9.

Department of Developmental Biology, Sloan-Kettering Institute, 1275 York Ave, Box 252, New York, NY 10065, USA

Photoreceptors in the crystalline eye are recruited by receptor tyrosine kinase (RTK)/Ras signaling mediated by Epidermal growth factor receptor (EGFR) and the Sevenless (Sev) receptor. Analyses of an allelic deletion series of the locus, along with a panel of modified genomic rescue transgenes, show that eye patterning depends on both miRNAs. Transcriptional reporter and activity sensor transgenes reveal expression and function of miR-279/996 in non-neural cells of the developing eye. Read More

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Targeted knock-in of CreER in zebrafish using CRISPR/Cas9.

Cell Tissue Res 2018 04 12;372(1):41-50. Epub 2018 Feb 12.

Center for Molecular and Cellular Bioengeneering (CMCB), DFG-Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Fetscherstr. 105, 01307, Dresden, Germany.

New genome-editing approaches, such as the CRISPR/Cas system, have opened up great opportunities to insert or delete genes at targeted loci and have revolutionized genetics in model organisms like the zebrafish. The Cre-loxp recombination system is widely used to activate or inactivate genes with high spatial and temporal specificity. Using a CRISPR/Cas9-mediated knock-in strategy, we inserted a zebrafish codon-optimized CreER transgene at the otx2 gene locus to generate a conditional Cre-driver line. Read More

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Conditional ablation of the RFX4 isoform 1 transcription factor: Allele dosage effects on brain phenotype.

PLoS One 2018 3;13(1):e0190561. Epub 2018 Jan 3.

Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, Unites States of America.

Regulatory factor X4 (RFX4) isoform 1 is a recently discovered isoform of the winged helix transcription factor RFX4, which can bind to X-box consensus sequences that are enriched in the promoters of cilia-related genes. Early insertional mutagenesis studies in mice first identified this isoform, and demonstrated that it was crucial for mouse brain development. RFX4 isoform 1 is the only RFX4 isoform significantly expressed in the mouse fetal and adult brain. Read More

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February 2018

Efficient transgenesis and annotated genome sequence of the regenerative flatworm model Macrostomum lignano.

Nat Commun 2017 12 14;8(1):2120. Epub 2017 Dec 14.

European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AV, Groningen, The Netherlands.

Regeneration-capable flatworms are informative research models to study the mechanisms of stem cell regulation, regeneration, and tissue patterning. However, the lack of transgenesis methods considerably hampers their wider use. Here we report development of a transgenesis method for Macrostomum lignano, a basal flatworm with excellent regeneration capacity. Read More

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December 2017

Gata6 restricts Isl1 to the posterior of nascent hindlimb buds through Isl1 cis-regulatory modules.

Dev Biol 2018 02 7;434(1):74-83. Epub 2017 Dec 7.

Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, United States; Stem Cell Institute, University of Minnesota, Minneapolis, MN, United States. Electronic address:

Isl1 is required for two processes during hindlimb development: initiation of the processes directing hindlimb development in the lateral plate mesoderm and configuring posterior hindlimb field in the nascent hindlimb buds. During these processes, Isl1 expression is restricted to the posterior mesenchyme of hindlimb buds. How this dynamic change in Isl1 expression is regulated remains unknown. Read More

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February 2018

A screen for E3 ubiquitination ligases that genetically interact with the adaptor protein Cindr during Drosophila eye patterning.

PLoS One 2017 8;12(11):e0187571. Epub 2017 Nov 8.

Biology Department, Wesleyan University, Middletown, Connecticut, United States of America.

Ubiquitination is a crucial post-translational modification that can target proteins for degradation. The E3 ubiquitin ligases are responsible for recognizing substrate proteins for ubiquitination, hence providing specificity to the process of protein degradation. Here, we describe a genetic modifier screen that identified E3 ligases that modified the rough-eye phenotype generated by expression of cindrRNAi transgenes during Drosophila eye development. Read More

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November 2017

The receptor protein tyrosine phosphatase PTPRB negatively regulates FGF2-dependent branching morphogenesis.

Development 2017 10 4;144(20):3777-3788. Epub 2017 Sep 4.

European Cancer Stem Cell Research Institute and Cardiff School of Biosciences, Cardiff University, Hadyn Ellis Building, Maindy Road, Cardiff, CF24 4HQ, UK

PTPRB is a transmembrane protein tyrosine phosphatase known to regulate blood vessel remodelling and angiogenesis. Here, we demonstrate that PTPRB negatively regulates branching morphogenesis in the mouse mammary epithelium. We show that is highly expressed in adult mammary stem cells and also, although at lower levels, in oestrogen receptor-positive luminal cells. Read More

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October 2017

Functional regulatory evolution outside of the minimal stripe 2 enhancer.

Development 2017 09 31;144(17):3095-3101. Epub 2017 Jul 31.

Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.

Transcriptional enhancers are regions of DNA that drive precise patterns of gene expression. Although many studies have elucidated how individual enhancers can evolve, most of this work has focused on what are called 'minimal' enhancers, the smallest DNA regions that drive expression that approximates an aspect of native gene expression. Here, we explore how the () locus has evolved by testing its activity in the divergent genome. Read More

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September 2017

Essential basal cytonemes take up Hedgehog in the wing imaginal disc.

Development 2017 09 25;144(17):3134-3144. Epub 2017 Jul 25.

Cardiovascular Research Institute, University of California, San Francisco, CA 94143, USA

Morphogen concentration gradients that extend across developmental fields form by dispersion from source cells. In the wing disc, Hedgehog (Hh) produced by posterior compartment cells distributes in a concentration gradient to adjacent cells of the anterior compartment. We monitored Hh:GFP after pulsed expression, and analyzed the movement and colocalization of Hh, Patched (Ptc) and Smoothened (Smo) proteins tagged with GFP or mCherry and expressed at physiological levels from bacterial artificial chromosome transgenes. Read More

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September 2017

BAC Recombineering of the Agouti Loci from Spotted Gar and Zebrafish Reveals the Evolutionary Ancestry of Dorsal-Ventral Pigment Asymmetry in Fish.

J Exp Zool B Mol Dev Evol 2017 Nov 24;328(7):697-708. Epub 2017 May 24.

Instituto de Investigaciones Marinas, CSIC, Vigo, Spain.

Dorsoventral pigment patterning, characterized by a light ventrum and a dark dorsum, is one of the most widespread chromatic adaptations in vertebrate body coloration. In mammals, this countershading depends on differential expression of agouti-signaling protein (ASIP), which drives a switch of synthesis of one type of melanin to another within melanocytes. Teleost fish share countershading, but the pattern results from a differential distribution of multiple types of chromatophores, with black-brown melanophores most abundant in the dorsal body and reflective iridophores most abundant in the ventral body. Read More

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November 2017