46,930 results match your criteria pathwayresults overexpressing

Atorvastatin Regulates MALAT1/miR-200c/NRF2 Activity to Protect Against Podocyte Pyroptosis Induced by High Glucose.

Diabetes Metab Syndr Obes 2021 13;14:1631-1645. Epub 2021 Apr 13.

Department of Endocrinology, Affiliated Hospital of Guilin Medical University, Guilin, 541001, People's Republic of China.

Background: Diabetic nephropathy (DN) is one of the main complications of diabetes mellitus (DM), which leads to the long-term loss of kidney functions. Long noncoding RNAs (LncRNAs) can alleviate DN by interacting with microRNAs (miRNAs). In this work, we aimed to explore the effects of the MALAT1/miR-200c/NRF2 regulatory axis on the pyroptosis and oxidative stress (Oxidative stress, OS) of renal podocytes in high glucose (HG) environment and whether the lipid-lowering drug atorvastatin (AT) can relieve renal OS through this approach. Read More

View Article and Full-Text PDF

suppresses proliferation and induces apoptosis of colorectal cancer cells by repressing oncogene .

Aging (Albany NY) 2021 Apr 20;13. Epub 2021 Apr 20.

Department of Colorectal Cancer Surgery, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Kunming 650118, China.

Colorectal cancer (CRC), a common malignant tumor in the digestive tract, is a leading cause of cancer-related death. has been reported to act as a tumor suppressor gene in various tumors. This study aims to assess the role of SPRY4 in colorectal cancer (CRC) and uncover its underlying mechanisms. Read More

View Article and Full-Text PDF

Antigen Expression Regulates CD4 T Cell Differentiation and Vaccine Efficacy against Mycobacterium tuberculosis Infection.

mBio 2021 Apr 20;12(2). Epub 2021 Apr 20.

Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark

New vaccines are urgently needed against (Mtb), which kills more than 1.4 million people each year. CD4 T cell differentiation is a key determinant of protective immunity against Mtb, but it is not fully understood how host-pathogen interactions shape individual antigen-specific T cell populations and their protective capacity. Read More

View Article and Full-Text PDF

Clinical and immunologic responses to a B-cell epitope vaccine in HER2/neu overexpressing advanced gastric cancer patients - results from Phase 1b trial IMU.ACS.001.

Clin Cancer Res 2021 Apr 20. Epub 2021 Apr 20.

Clinical Development, Imugene Limited.

Background: HER2/neu is overexpressed in up to 30% of gastroesophageal adenocarcinomas (GEA) and linked to poor prognosis. Recombinant mAbs to treat HER2/neu-overexpressing cancers are effective with limitations, including resistance and toxicity. Therefore, we developed a therapeutic B-cell epitope vaccine (IMU-131/HER-Vaxx) consisting of 3 fused B-cell epitopes from the HER2/neu extracellular domain coupled to CRM197 and adjuvanted with Montanide. Read More

View Article and Full-Text PDF

The effect of miR-539 regulating TRIAP1 on the apoptosis, proliferation, migration and invasion of osteosarcoma cells.

Cancer Cell Int 2021 Apr 20;21(1):227. Epub 2021 Apr 20.

Department of Spine Surgery and Musculoskeletal Tumor, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.

Objective: The purpose of this study is to explore the effect of miRNA-539 on osteosarcoma (OS) and the underlying mechanism, so as to find a new method for early diagnosis and treatment of osteosarcoma.

Method: miRNA-539 mimics was transfected into osteosarcoma cells 143b and MG-63 and upregulated the expression of miR-539. QT-PCR was used to detect transfection efficacy. Read More

View Article and Full-Text PDF

Identification of the transcription factor, AFF4, as a new target of miR-203 in CNS.

Int J Biol Macromol 2021 Apr 17. Epub 2021 Apr 17.

The Key Laboratory of Virology of Guangzhou, Jinan University, Guangzhou, China. Electronic address:

MiR-203 was identified as a hub of a potential regulatory miRNA network in central nervous system. Overexpressing of miR-203 in the frontal cortex of C57BL/6J wild type mouse induced neurodegeneration by increasing the apoptotic pathway and neuron death. AFF4, a transcription factor, was identified as a new bona fida protein target of miR-203 in CNS. Read More

View Article and Full-Text PDF

Membrane-Bound Transcriptional Activator NTL1 from Rapeseed Positively Modulates Leaf Senescence through Targeting Genes Involved in Reactive Oxygen Species Production and Programmed Cell Death.

J Agric Food Chem 2021 Apr 20. Epub 2021 Apr 20.

State Key Laboratory of Crop Stress Biology for Arid Areas and, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China.

Leaf senescence is the last stage of leaf development and is determined by various environmental and endogenous signals. Leaf senescence can determine plant productivity and fitness. Transcription factors (TFs) with the transmembrane domain constitute a special group of regulatory proteins that can translocate from the membrane system into nuclei to exert the transcriptional function upon endogenous or exogenous stimuli. Read More

View Article and Full-Text PDF

The transcription factor gene RDD4 contributes to the control of nutrient ion accumulation in rice.

Physiol Plant 2021 Apr 20. Epub 2021 Apr 20.

Division of Applied Genetics, Institute of Agrobiological Sciences, NARO, Tsukuba, Ibaraki, Japan.

In this study, we investigated the expression and functions of the transcription factor gene RDD4 (rice Dof daily fluctuations 4), which has sequence similarity to RDD1 that controls nutrient ion accumulation in rice. RDD4 protein was highly accumulated in leaf sheaths and localized to vascular bundles. RDD4-overexpressing plants (RDD4-OX) improved the accumulation of various nutrient ions, irrespective of nutrient concentration in a hydroponic solution. Read More

View Article and Full-Text PDF

Anti-Human Epidermal Growth Factor Receptor 2 Single-Chain Fv Fragment-Decorated DM1 Nanoparticles for Specific Targeting of Human Epidermal Growth Factor Receptor 2-Positive Breast Tumor Cells.

Ling Ni You-Xin Li

J Biomed Nanotechnol 2021 Mar;17(3):447-455

Purpose: Although monoclonal antibodies are used to decorate nanoparticles to target specific cells, penetration of tumor tissues by monoclonal antibodies is limited by their large size. Therefore, we prepared DM1 nanoparticles decorated with the small anti-HER2 single-chain Fv fragment (scFvHER2) of trastuzumab (TMAB) for targeting to human epidermal growth factor receptor 2 (HER2) overexpressing in breast cancer effectively.

Methods: ScFvHER2 fragment was coupled with DM1 nanoparticles (NPs) via covalent thiol-maleimide linkages. Read More

View Article and Full-Text PDF

Damage to cardiac vasculature may be associated with breast cancer treatment-induced cardiotoxicity.

Cardiooncology 2021 Apr 19;7(1):15. Epub 2021 Apr 19.

Department of Cancer Biology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA.

Background: Breast cancer is the most common female cancer worldwide. Effective therapies including doxorubicin and trastuzumab have improved survival, but are associated with a substantial risk of cardiovascular disease. Mechanisms underlying cancer treatment-induced cardiotoxicity (CTC) are poorly understood and have largely focused on cardiomyocyte damage, although other cellular populations in the heart such as the cardiac endothelium, may play an important role in cardiac damage. Read More

View Article and Full-Text PDF

Improved growth in a field trial of transgenic hybrid poplar overexpressing glutamine synthetase.

New Phytol 2004 Oct;164(1):137-145

Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias e Instituto Andaluz de Biotecnología, Unidad asociada UMA-CSIC, Universidad de Málaga. Campus de Teatinos, 29071 Málaga, Spain.

•  In the present work the performance of transgenic poplars expressing a pine glutamine synthetase (GS) transgene was studied in natural conditions. •  A field study of eight independent transgenic lines and control plants was carried out for 3 yr in the province of Granada (Spain). •  Transgenic poplars reached average heights that were 21, 36 and 41% greater than control plants after the first, second and third year of growth, respectively. Read More

View Article and Full-Text PDF
October 2004

Cancer Stem Cell-Targeted Gene Delivery Mediated by Aptamer-Decorated pH-Sensitive Nanoliposomes.

ACS Biomater Sci Eng 2021 Apr 19. Epub 2021 Apr 19.

Technical Research Centre, Indian Association for the Cultivation of Science, Kolkata 700032, India.

A new pH-responsive cationic co-liposomal formulation was prepared in this study using the twin version of the amphiphile palmitoyl homocysteine, TPHC; natural zwitterionic lipid, DOPE; and cholesterol-based twin cationic lipid, C5C, at specified molar ratios. This co-liposome was further decorated with a newly designed fluorescently tagged, cholesterol-tethered EpCAM-targeting RNA aptamer for targeted gene delivery. This aptamer-guided nanoliposomal formulation, C5C/DOPE/TPHC at 8:24:1 molar ratio, could efficiently transport the genes in response to low pH of cellular endosomes selectively to the EpCAM overexpressing cancer stem cells. Read More

View Article and Full-Text PDF

Hyperactivity is a Core Endophenotype of Elevated Neuregulin-1 Signaling in Embryonic Glutamatergic Networks.

Schizophr Bull 2021 Apr 19. Epub 2021 Apr 19.

Department of Neurogenetics, Max-Planck-Institute of Experimental Medicine, Goettingen, Germany.

The neuregulin 1 (NRG1) ErbB4 module is at the core of an "at risk" signaling pathway in schizophrenia. Several human studies suggest hyperstimulation of NRG1-ErbB4 signaling as a plausible pathomechanism; however, little is known about the significance of stage-, brain area-, or neural cell type-specific NRG1-ErbB4 hyperactivity for disease-relevant brain endophenotypes. To address these spatiotemporal aspects, we generated transgenic mice for Cre recombinase-mediated overexpression of cystein-rich domain (CRD) NRG1, the most prominent NRG1 isoform in the brain. Read More

View Article and Full-Text PDF

HOTAIR-Loaded Mesenchymal Stem/Stromal Cell Extracellular Vesicles Enhance Angiogenesis and Wound Healing.

Adv Healthc Mater 2021 Apr 18:e2002070. Epub 2021 Apr 18.

Fischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.

Chronic wounds remain a substantial source of morbidity worldwide. An emergent approach that may be well-suited to induce the complex, multicellular processes such as angiogenesis that are required for wound repair is the use of extracellular vesicles (EVs). EVs contain a wide variety of proteins and nucleic acids that enable multifactorial signaling. Read More

View Article and Full-Text PDF

Effects of FER1L4-miR-106a-5p/miR-372-5p-E2F1 regulatory axis on drug resistance in liver cancer chemotherapy.

Mol Ther Nucleic Acids 2021 Jun 10;24:449-461. Epub 2021 Feb 10.

Ultrasound in Cardiac Electrophysiology and Biomechanics Key Laboratory of Sichuan Province, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China and Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu 610072, P.R. China.

Liver cancer presents a challenge in today's healthcare system. This study aimed at investigating the effects of Fer-1 like family member 4 (FER1L4) on chemotherapy resistance and liver cancer development by using clinically collected liver cancer tissues and commercially purchased human liver cancer cisplatin-resistant cell line HUH-7/DDP. Bioinformatics analysis, dual luciferase reporter gene assay, and RNA pull-down were applied to predict and verify the possible binding relationships. Read More

View Article and Full-Text PDF

Overexpression of a Cytochrome P450 Monooxygenase Involved in Orobanchol Biosynthesis Increases Susceptibility to Fusarium Head Blight.

Front Plant Sci 2021 1;12:662025. Epub 2021 Apr 1.

Université Paris-Saclay, CNRS, INRAE, University of Evry, Institute of Plant Sciences Paris-Saclay, Orsay, France.

Fusarium Head Blight (FHB) is a cereal disease caused primarily by the ascomycete fungus with public health issues due to the production of mycotoxins including deoxynivalenol (DON). Genetic resistance is an efficient protection means and numerous quantitative trait loci have been identified, some of them related to the production of resistance metabolites. In this study, we have functionally characterized the gene encoding a cytochrome P450 monooxygenase (CYP). Read More

View Article and Full-Text PDF

MiR-22 Inhibition Alleviates Cardiac Dysfunction in Doxorubicin-Induced Cardiomyopathy by Targeting the sirt1/PGC-1α Pathway.

Front Physiol 2021 1;12:646903. Epub 2021 Apr 1.

Department of Cardiology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.

Doxorubicin (DOX) cardiotoxicity is a life-threatening side effect that leads to a poor prognosis in patients receiving chemotherapy. We investigated the role of miR-22 in doxorubicin-induced cardiomyopathy and the underlying mechanism and . Specifically, we designed loss-of-function and gain-of-function experiments to identify the role of miR-22 in doxorubicin-induced cardiomyopathy. Read More

View Article and Full-Text PDF

Subtle Role for Adenylate Kinase 1 in Maintaining Normal Basal Contractile Function and Metabolism in the Murine Heart.

Front Physiol 2021 31;12:623969. Epub 2021 Mar 31.

Division of Cardiovascular Medicine, Radcliffe Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.

Aims: Adenylate kinase 1 (AK1) catalyses the reaction 2ADP ↔ ATP + AMP, extracting extra energy under metabolic stress and promoting energetic homeostasis. We hypothesised that increased AK1 activity would have negligible effects at rest, but protect against ischaemia/reperfusion (I/R) injury.

Methods And Results: Cardiac-specific AK1 overexpressing mice (AK1-OE) had 31% higher AK1 activity ( = 0. Read More

View Article and Full-Text PDF

STIM1 promotes IPEC-J2 porcine epithelial cell restitution by TRPC1 signaling.

Anim Biotechnol 2021 Apr 18:1-12. Epub 2021 Apr 18.

Maize Research Institute, Sichuan Agricultural University, Chengdu, China.

Intestinal epithelial restitution is partly dependent on cell migration, which reseals superficial wounding after injury. Here, we tested the hypothesis that stromal interaction molecule 1(STIM1) regulates porcine intestinal epithelial cell migration by activating transient receptor potential canonical 1 (TRPC1) signaling. Results showed that the knockdown of STIM1 repressed cell migration after wounding, reduced the protein concentration of STIM1 and TRPC1, and decreased the inositol trisphosphate (IP3) content in IPEC-J2 cells ( < 0. Read More

View Article and Full-Text PDF

Dietary-challenged mice with Alzheimer-like pathology show increased energy expenditure and reduced adipocyte hypertrophy and steatosis.

Aging (Albany NY) 2021 Apr 16;13. Epub 2021 Apr 16.

Department of Endocrinology and Metabolism, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin 10117, Germany.

Alzheimer's disease (AD) is frequently accompanied by progressing weight loss, correlating with mortality. Counter-intuitively, weight loss in old age might predict AD onset but obesity in midlife increases AD risk. Furthermore, AD is associated with diabetes-like alterations in glucose metabolism. Read More

View Article and Full-Text PDF

Human TRIM14 protects transgenic mice from influenza A viral infection without activation of other innate immunity pathways.

Genes Immun 2021 Apr 16. Epub 2021 Apr 16.

Department of Viral and Cellular Molecular Genetics, Institute of Molecular Genetics of National Research Centre "Kurchatov Institute", Moscow, Russia.

TRIM14 is an important component of innate immunity that defends organism from various viruses. It was shown that TRIM14 restricted influenza A virus (IAV) infection in cell cultures in an interferon-independent manner. However, it remained unclear whether TRIM14 affects IAV reproduction and immune system responses upon IAV infection in vivo. Read More

View Article and Full-Text PDF

Patient-specific iPSC-derived cellular models of LGMDR1.

Stem Cell Res 2021 Apr 8;53:102333. Epub 2021 Apr 8.

Neuroscience Area, Biodonostia Research Institute, San Sebastian, Spain; CIBERNED, Instituto de Salud Carlos III, Madrid, Spain; Department of Neuroscience, University of the Basque Country, San Sebastian, Spain; Department of Neurology, Hospital Universitario Donostia, San Sebastian, Spain. Electronic address:

Limb-girdle muscular dystrophy recessive 1 (LGMDR1) represents one of the most common types of LGMD in the population, where patients develop a progressive muscle degeneration. The disease is caused by mutations in calpain 3 gene, with over 500 mutations reported to date. However, the molecular events that lead to muscle wasting are not clear, nor the reasons for the great clinical variability among patients, and this has so far hindered the development of effective therapies. Read More

View Article and Full-Text PDF

HY5 regulates light-responsive transcription of microRNA163 to promote primary root elongation in Arabidopsis seedlings.

J Integr Plant Biol 2021 Apr 16. Epub 2021 Apr 16.

College of Life Science, Henan Agricultural University, Zhengzhou, 450002, China.

MicroRNAs (miRNAs) play key roles in the post-transcriptional regulation of gene expression in plants. Many miRNAs are responsive to environmental signals. Light is the first environmental signal perceived by plants after emergence from the soil. Read More

View Article and Full-Text PDF

Transcriptional Enhancer Factor Domain Family member 4 Exerts an Oncogenic Role in Hepatocellular Carcinoma by Hippo-Independent Regulation of Heat Shock Protein 70 Family Members.

Hepatol Commun 2021 Apr 20;5(4):661-674. Epub 2021 Jan 20.

Institute of Medical Genetics and Pathology University Hospital Basel Basel Switzerland.

Transcriptional enhancer factor domain family member 4 (TEAD4) is a downstream effector of the conserved Hippo signaling pathway, regulating the expression of genes involved in cell proliferation and differentiation. It is up-regulated in several cancer types and is associated with metastasis and poor prognosis. However, its role in hepatocellular carcinoma (HCC) remains largely unexplored. Read More

View Article and Full-Text PDF

STAT3 ameliorates cognitive deficits via regulation of NMDAR expression in an Alzheimer's disease animal model.

Theranostics 2021 13;11(11):5511-5524. Epub 2021 Mar 13.

Department of Pathophysiology, School of Basic Medicine and the Collaborative Innovation Center for Brain Science, Key Laboratory of Ministry of Education of China and Hubei Province for Neurological Disorders, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Abnormal tau accumulation in the brain has a positively correlation with neurodegeneration and memory deterioration, but the mechanism underlying tau-associated synaptic and cognitive impairments remains unclear. Our previous work has found that human full length tau (hTau) accumulation activated signal transducer and activator of transcription-1 (STAT1) to suppress N-methyl-D-aspartate receptors (NMDARs) expression, followed by memory deficits. STAT3 also belongs to STAT protein family and is reported to involve in regulation of synaptic plasticity and cognition. Read More

View Article and Full-Text PDF

CD45ROCD8 T cell-derived exosomes restrict estrogen-driven endometrial cancer development via the ERβ/miR-765/PLP2/Notch axis.

Theranostics 2021 11;11(11):5330-5345. Epub 2021 Mar 11.

Laboratory for Reproductive Immunology, NHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Hospital of Obstetrics and Gynecology, Shanghai Medical School, Fudan University, Shanghai 200080, People's Republic of China.

Estrogen-dependent cancers (e.g., breast, endometrial, and ovarian cancers) are among the leading causes of morbidity and mortality in women worldwide. Read More

View Article and Full-Text PDF

Preventing tumor progression to the bone by induced tumor-suppressing MSCs.

Theranostics 2021 5;11(11):5143-5159. Epub 2021 Mar 5.

Department of Pharmacology, College of Pharmacy, Harbin Medical University, Harbin 150081, China.

Advanced breast cancer metastasizes to many organs including bone, but few effective treatments are available. Here we report that induced tumor-suppressing (iTS) MSCs protected bone from metastases while un-induced MSCs did not. iTS MSCs were generated by overexpressing Lrp5, β-catenin, Snail, or Akt. Read More

View Article and Full-Text PDF

Characterization and Mechanism of Linearized-Microcystinase Involved in Bacterial Degradation of Microcystins.

Front Microbiol 2021 30;12:646084. Epub 2021 Mar 30.

Hunan Provincial Key Laboratory of Clinical Epidemiology, Xiangya School of Public Health, Central South University, Changsha, China.

Microcystins (MCs) are extremely hazardous to the ecological environment and public health. How to control and remove MCs is an unsolved problem all over the world. Some microbes and their enzymes are thought to be effective in degrading MCs. Read More

View Article and Full-Text PDF

Activation of nuclear factor-kappa B by TNF promotes nucleus pulposus mineralization through inhibition of ANKH and ENPP1.

Sci Rep 2021 Apr 15;11(1):8271. Epub 2021 Apr 15.

HSS Research Institute, Hospital for Special Surgery, 515 E 71st Street, New York, NY, 10021, USA.

Spontaneous mineralization of the nucleus pulposus (NP) has been observed in cases of intervertebral disc degeneration (IDD). Inflammatory cytokines have been implicated in mineralization of multiple tissues through their modulation of expression of factors that enable or inhibit mineralization, including TNAP, ANKH or ENPP1. This study examines the underlying factors leading to NP mineralization, focusing on the contribution of the inflammatory cytokine, TNF, to this pathologic event. Read More

View Article and Full-Text PDF

Selenoprotein W ensures physiological bone remodeling by preventing hyperactivity of osteoclasts.

Nat Commun 2021 04 15;12(1):2258. Epub 2021 Apr 15.

Department of Microbiology, Laboratory of Bone Metabolism and Control, Yeungnam University College of Medicine, Daegu, Korea.

Selenoproteins containing selenium in the form of selenocysteine are critical for bone remodeling. However, their underlying mechanism of action is not fully understood. Herein, we report the identification of selenoprotein W (SELENOW) through large-scale mRNA profiling of receptor activator of nuclear factor (NF)-κΒ ligand (RANKL)-induced osteoclast differentiation, as a protein that is downregulated via RANKL/RANK/tumour necrosis factor receptor-associated factor 6/p38 signaling. Read More

View Article and Full-Text PDF