3,106 results match your criteria passive dna

Novel Pyrimidine Derivatives as Potential Anticancer Agents: Synthesis, Biological Evaluation and Molecular Docking Study.

Int J Mol Sci 2021 Apr 7;22(8). Epub 2021 Apr 7.

Department of Inorganic Chemistry, Wroclaw Medical University, Borowska 211A, Borowska 211A, 50-556 Wroclaw, Poland.

In the present paper, new pyrimidine derivatives were designed, synthesized and analyzed in terms of their anticancer properties. The tested compounds were evaluated in vitro for their antitumor activity. The cytotoxic effect on normal human dermal fibroblasts (NHDF) was also determined. Read More

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Efficacy of Birth Dose Vaccination in Preventing Mother-to-Child Transmission of Hepatitis B: A Randomized Controlled Trial Comparing Engerix-B and Sci-B-Vac.

Vaccines (Basel) 2021 Apr 1;9(4). Epub 2021 Apr 1.

National Reference Center for Hepatitis B Viruses and Hepatitis D Viruses, Institute of Medical Virology, Justus Liebig University Giessen, 35392 Giessen, Germany.

Peripartum transmission of hepatitis B virus (HBV) from an infected mother to the child can be prevented in most but not all cases by immediate vaccination of the newborn. The aim of this study was to compare the efficacy of two licensed hepatitis B vaccines, Engerix-B versus Sci-B-Vac, in preventing peripartum HBV transmission. A prospective multicenter randomized controlled study in 4 delivery centers was performed from 2009 to 2014. Read More

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Bromelain and Olea europaea (L.) leaf extract mediated alleviation of benzo(a)pyrene induced lung cancer through Nrf2 and NFκB pathway.

Environ Sci Pollut Res Int 2021 Apr 23. Epub 2021 Apr 23.

Immunology Microbiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar Tripura, 799022, India.

Lung cancer is the most aggressive as well as deadly form of cancer and most of the lung cancer cases are involved in direct smoking or passive smoking. Oxidative stress and pulmonary inflammation regulated by some transcription factors like Nrf2, NF-κB etc. play important roles in lung cancer. Read More

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Nuclease Hydrolysis Does Not Drive the Rapid Signaling Decay of DNA Aptamer-Based Electrochemical Sensors in Biological Fluids.

Langmuir 2021 May 20;37(17):5213-5221. Epub 2021 Apr 20.

Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21202, United States.

Electrochemical aptamer-based (E-AB) sensors are a technology capable of real-time monitoring of drug concentrations directly in the body. These sensors achieve their selectivity from surface-attached aptamers, which alter their conformation upon target binding, thereby causing a change in electron transfer kinetics between aptamer-bound redox reporters and the electrode surface. Because, in theory, aptamers can be selected for nearly any target of interest, E-AB sensors have far-reaching potential for diagnostic and biomedical applications. Read More

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Mild Leber hereditary optic neuropathy (LHON) in a Western European family due to the rare Asian m.14502T>C variant in the gene.

Ophthalmic Genet 2021 Apr 16:1-6. Epub 2021 Apr 16.

Department of Ophthalmology, Ghent University Hospital, Ghent, Belgium.

: Leber hereditary optic neuropathy (LHON) is a mitochondrial neurodegenerative disease. The majority (>90%) is related to three primary mitochondrial DNA (mtDNA) variants: m.3460G>A, m. Read More

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Diabetic Encephalopathy Affecting Mitochondria and Axonal Transport Proteins.

Basic Clin Neurosci 2020 Nov-Dec;11(6):781-793. Epub 2020 Nov 1.

Departmentof Physiology, Neurophysiology Research Center, Shahed University, Tehran, Iran.

Introduction: Diabetic encephalopathy is described as any cognitive and memory impairments associated with hippocampal degenerative changes, including the neurodegenerative process and decreased number of living cells. Mitochondrial diabetes (MD) appears following activation of mutant mitochondrial DNA and is a combination of diabetes and cognitive deficit. In this research, we showed the correlation of diabetic encephalopathy, dysfunctional mitochondria, and changes in the expression of axonal transport proteins (KIF5b, Dynein). Read More

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November 2020

DNA methylation of GFI1 as a mediator of the association between prenatal smoking exposure and ADHD symptoms at 6 years: the Hokkaido Study on Environment and Children's Health.

Clin Epigenetics 2021 Apr 7;13(1):74. Epub 2021 Apr 7.

Center for Environmental and Health Sciences, Hokkaido University, Hokkaido, Japan.

Background: Prenatal smoking exposure has been associated with childhood attention-deficit/hyperactivity disorder (ADHD). However, the mechanism underlying this relationship remains unclear. We assessed whether DNA methylation differences may mediate the association between prenatal smoking exposure and ADHD symptoms at the age of 6 years. Read More

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Author Correction: Passive eDNA collection enhances aquatic biodiversity analysis.

Commun Biol 2021 Apr 6;4(1):447. Epub 2021 Apr 6.

Commonwealth Scientific and Industrial Research Organization, Indian Oceans Marine Research Centre, Environomics Future Science Platform, Crawley, WA, Australia.

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High-throughput human DNA purification on a centrifugal microfluidic device for rapid forensic sex-typing.

Biosens Bioelectron 2021 Jun 13;181:113161. Epub 2021 Mar 13.

Department of Chemical Engineering (BK21 FOUR Integrated Engineering Program), Kyung Hee University, Yongin, 17104, South Korea. Electronic address:

We introduce a novel centrifugal disc for purifying nucleic acid (NA) in a high-throughput manner to perform the human sex-typing of forensic samples. The centrifugal disc was designed with double-sided etched channels to fabricate 30 extraction units, which was capable of purifying 30 forensic samples in a single run. In order to introduce the washing solution (70% ethanol) and the elution buffer for the 30 extraction units in an automatic manner, we designed the aliquoting chambers that were connected with a zigzag delivery channel. Read More

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Nucleoside Analogs Radiosensitize G0 Cells by Activating DNA End Resection and Alternative End-Joining.

Radiat Res 2021 Mar 23. Epub 2021 Mar 23.

Institute of Medical Radiation Biology, University of Duisburg-Essen Medical School, Essen, Germany.

Alternative end-joining (alt-EJ) is a DNA end resection-dependent, error-prone pathway utilized by vertebrate cells to repair DNA double-strand breaks (DSBs), but its engagement is linked to chromosomal translocations and genomic instability. Here, we report that when proliferating cells are exposed to ionizing radiation, treatment with nucleoside analogs (NAs) causes strong radiosensitization by increasing engagement of alt-EJ, while at the same time suppressing homologous recombination (HR) in S- and G2-phase cells. This NA-mediated pathway shift may reflect a passive compensatory engagement of alt-EJ following HR suppression that is specific for S- and G2-phase cells, and/or the direct activation of alt-EJ throughout the cell cycle. Read More

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The role of migration barriers for dispersion of Proliferative Kidney Disease-Balance between disease emergence and habitat connectivity.

PLoS One 2021 17;16(3):e0247482. Epub 2021 Mar 17.

FishConsulting GmbH, Olten, Switzerland.

Natural and uninterrupted water courses are important for biodiversity and fish population stability. Nowadays, many streams and rivers are obstructed by artificial migration barriers, often preventing the migration of fish. On the other hand, distribution of pathogens by migrating fishes is still a point of concern. Read More

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Synthetic DNA Delivery of an Optimized and Engineered Monoclonal Antibody Provides Rapid and Prolonged Protection against Experimental Gonococcal Infection.

mBio 2021 03 16;12(2). Epub 2021 Mar 16.

Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts, USA

Monoclonal antibody (MAb) 2C7 recognizes a lipooligosaccharide epitope expressed by most clinical isolates and mediates complement-dependent bactericidal activity. We recently showed that a recombinant human IgG1 chimeric variant of MAb 2C7 containing an E430G Fc modification (2C7_E430G), which enhances complement activation, outperformed the parental MAb 2C7 (2C7_WT) Because natural infection with often does not elicit protective immunity and reinfections are common, approaches that prolong bacterial control are of great interest. Advances in DNA-based approaches have demonstrated the combined benefit of genetic engineering, formulation optimizations, and facilitated delivery via CELLECTRA-EP technology, which can induce robust expression of protective DNA-encoded monoclonal antibodies (DMAbs) with durable serum activity relative to traditional recombinant MAb therapies. Read More

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Assessment of Human Papillomavirus Infection and Risk Factors in Egyptian Women With Breast Cancer.

Breast Cancer (Auckl) 2021 25;15:1178223421996279. Epub 2021 Feb 25.

Department of Laboratory Medicine and Pathology, Mayo Clinic, Jacksonville, FL, USA.

Numerous risk factors for breast cancer (BC) have been identified. High-risk human papilloma virus (HR-HPV) is the etiological agent of cervical cancer and in some cases of head and neck cancer, specifically oropharyngeal cancer, but the role of HR-HPV in evoking neoplasia in BC is still unclear. In this study, all women above the age of 18 visiting the oncology clinic at Al-Azhar university hospital and Ain Shams specialized hospital between the period of February 2017 and March 2018 were invited to participate. Read More

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February 2021

One or two injections of MVA-vectored vaccine shields hACE2 transgenic mice from SARS-CoV-2 upper and lower respiratory tract infection.

Proc Natl Acad Sci U S A 2021 03;118(12)

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892

Modified vaccinia virus Ankara (MVA) is a replication-restricted smallpox vaccine, and numerous clinical studies of recombinant MVAs (rMVAs) as vectors for prevention of other infectious diseases, including COVID-19, are in progress. Here, we characterize rMVAs expressing the S protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Modifications of full-length S individually or in combination included two proline substitutions, mutations of the furin recognition site, and deletion of the endoplasmic retrieval signal. Read More

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Novel diagnostic techniques and therapeutic strategies for IgE-mediated food allergy.

Allergy Asthma Proc 2021 03;42(2):124-130

Immunoglobulin E (IgE) mediated food allergy is a potentially life-threatening condition and represents a heavy burden for patients and their families. Identification of the most suitable way for management of each patient has currently become the primary goal for physicians. This study reviewed the current literature related to IgE-mediated food allergy. Read More

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Maternal hepatitis B e antigen can be an indicator for antiviral prophylaxis of perinatal transmission of hepatitis B virus.

Emerg Microbes Infect 2021 Dec;10(1):555-564

Department of Microbiology & Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People's Republic of China.

As a high-risk factor of perinatal HBV transmission, the potential role of maternal hepatitis B e antigen (HBeAg) to guide antiviral prophylaxis has not yet been fully reported. This large prospective cohort study enrolled 1177 hepatitis B surface antigen (HBsAg)-positive pregnant women without antiviral treatment and their newborns. HBeAg, HBsAg, and viral load in maternal serum collected before delivery were measured. Read More

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December 2021

On the road to ending the COVID-19 pandemic: Are we there yet?

Virology 2021 05 26;557:70-85. Epub 2021 Feb 26.

Department of Medicine, Washington University School of Medicine, St. Louis, MO, 63110, USA; Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, 63110, USA; Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, 63110, USA; The Andrew M. and Jane M. Bursky Center for Human Immunology & Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO, 63110, USA. Electronic address:

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged into the human population in late 2019 and caused the global COVID-19 pandemic. SARS-CoV-2 has spread to more than 215 countries and infected many millions of people. Despite the introduction of numerous governmental and public health measures to control disease spread, infections continue at an unabated pace, suggesting that effective vaccines and antiviral drugs will be required to curtail disease, end the pandemic, and restore societal norms. Read More

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Design of Thioether Cyclic Peptide Scaffolds with Passive Permeability and Oral Exposure.

J Med Chem 2021 Mar 25;64(5):2622-2633. Epub 2021 Feb 25.

Novartis Institutes for BioMedical Research, 181 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.

Advances in the design of permeable peptides and in the synthesis of large arrays of macrocyclic peptides with diverse amino acids have evolved on parallel but independent tracks. Less precedent combines their respective attributes, thereby limiting the potential to identify permeable peptide ligands for key targets. Herein, we present novel 6-, 7-, and 8-mer cyclic peptides (MW 774-1076 g·mol) with passive permeability and oral exposure that feature the amino acids and thioether ring-closing common to large array formats, including DNA- and RNA-templated synthesis. Read More

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Modeling heritability of temperamental differences, stress reactivity, and risk for anxiety and depression: Relevance to research domain criteria (RDoC).

Eur J Neurosci 2021 Feb 24. Epub 2021 Feb 24.

School of Neuroscience, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.

Animal models provide important tools to study biological and environmental factors that shape brain function and behavior. These models can be effectively leveraged by drawing on concepts from the National Institute of Mental Health Research Domain Criteria (RDoC) Initiative, which aims to delineate molecular pathways and neural circuits that underpin behavioral anomalies that transcend psychiatric conditions. To study factors that contribute to individual differences in emotionality and stress reactivity, our laboratory utilized Sprague-Dawley rats that were selectively bred for differences in novelty exploration. Read More

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February 2021

Strand-specific single-cell methylomics reveals distinct modes of DNA demethylation dynamics during early mammalian development.

Nat Commun 2021 02 24;12(1):1286. Epub 2021 Feb 24.

Oncode Institute, Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht, Utrecht, The Netherlands.

DNA methylation (5mC) is central to cellular identity. The global erasure of 5mC from the parental genomes during preimplantation mammalian development is critical to reset the methylome of gametes to the cells in the blastocyst. While active and passive modes of demethylation have both been suggested to play a role in this process, the relative contribution of these two mechanisms to 5mC erasure remains unclear. Read More

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February 2021

Au-siRNA@ aptamer nanocages as a high-efficiency drug and gene delivery system for targeted lung cancer therapy.

J Nanobiotechnology 2021 Feb 24;19(1):54. Epub 2021 Feb 24.

Institute of Nano Biomedicine and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of the Ministry of Education, Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, Department of Instrument Science & Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People's Republic of China.

Background: Gene and chemical therapy has become one of the rising stars in the field of molecular medicine during the last two decades. However, there are still numerous challenges in the development of efficient, targeted, and safe delivery systems that can avoid siRNA degradation and reduce the toxicity and adverse effects of chemotherapy medicine.

Results: In this paper, a highly efficient AS1411 aptamer modified, dsDNA and MMP-2 cleavable peptide-fabricated gold nanocage vehicle, which could load doxorubicin hydrochloride (DOX) and siRNAs to achieve a combination of tumor responsive genetic therapy, chemotherapy, and photothermal treatment is presented. Read More

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February 2021

Passive eDNA collection enhances aquatic biodiversity analysis.

Commun Biol 2021 Feb 22;4(1):236. Epub 2021 Feb 22.

Commonwealth Scientific and Industrial Research Organization, Indian Oceans Marine Research Centre, Environomics Future Science Platform, Crawley, WA, Australia.

Environmental DNA (eDNA) metabarcoding is a sensitive and widely used approach for species detection and biodiversity assessment. The most common eDNA collection method in aquatic systems is actively filtering water through a membrane, which is time consuming and requires specialized equipment. Ecological studies investigating species abundance or distribution often require more samples than can be practically collected with current filtration methods. Read More

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February 2021

Quantifying Live Microbial Load in Human Saliva Samples over Time Reveals Stable Composition and Dynamic Load.

mSystems 2021 Feb 16;6(1). Epub 2021 Feb 16.

Department of Pediatrics, University of California, San Diego, La Jolla, California, USA

Evaluating microbial community composition through next-generation sequencing has become increasingly accessible. However, metagenomic sequencing data sets provide researchers with only a snapshot of a dynamic ecosystem and do not provide information about the total microbial number, or load, of a sample. Additionally, DNA can be detected long after a microorganism is dead, making it unsafe to assume that all microbial sequences detected in a community came from living organisms. Read More

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February 2021

Treatment and control strategies for COVID-19: Prospects and possibilities.

Pak J Pharm Sci 2020 Jul;33(4):1727-1730

Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan.

COVID-19 is a highly contagious and infectious disease, spreading rapidly worldwide. Its high spread rate has turned it into public stigma. The current study is focusing on its treatment and control strategies on the basis of the facts and figs obtained from previous studies. Read More

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Spontaneous and frequent conformational dynamics induced by A…A mismatch in d(CAA)·d(TAG) duplex.

Sci Rep 2021 Feb 11;11(1):3689. Epub 2021 Feb 11.

Department of Biotechnology, Indian Institute of Technology Hyderabad, Kandi, Sangareddy District, Telangana State, 502285, India.

Base pair mismatches in DNA can erroneously be incorporated during replication, recombination, etc. Here, the influence of A…A mismatch in the context of 5'CAA·5'TAG sequence is explored using molecular dynamics (MD) simulation, umbrella sampling MD, circular dichroism (CD), microscale thermophoresis (MST) and NMR techniques. MD simulations reveal that the A…A mismatch experiences several transient events such as base flipping, base extrusion, etc. Read More

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February 2021

Resilience to Stress: Lessons from Rodents about Nature versus Nurture.

Neuroscientist 2021 Feb 10:1073858421989357. Epub 2021 Feb 10.

School of Neuroscience, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.

Individual differences in human temperament influence how we respond to stress and can confer vulnerability (or resilience) to emotional disorders. For example, high levels of behavioral inhibition in children predict increased risk of mood and anxiety disorders in later life. The biological underpinnings of temperament are unknown, although improved understanding can offer insight into the pathogenesis of emotional disorders. Read More

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February 2021

DNA Demethylation in Response to Heat Stress in .

Int J Mol Sci 2021 Feb 4;22(4). Epub 2021 Feb 4.

Centre for Bioinformatics and Data Analysis, Medical University of Bialystok, 15-089 Bialystok, Poland.

Environmental stress is one of the most important factors affecting plant growth and development. Recent studies have shown that epigenetic mechanisms, such as DNA methylation, play a key role in adapting plants to stress conditions. Here, we analyzed the dynamics of changes in the level of DNA methylation in (L. Read More

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February 2021

Active and Passive Destabilization of G-Quadruplex DNA by the Telomere POT1-TPP1 Complex.

J Mol Biol 2021 Apr 4;433(7):166846. Epub 2021 Feb 4.

Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106, USA; Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106, USA. Electronic address:

Chromosome ends are protected by guanosine-rich telomere DNA that forms stable G-quadruplex (G4) structures. The heterodimeric POT1-TPP1 complex interacts specifically with telomere DNA to shield it from illicit DNA damage repair and to resolve secondary structure that impedes telomere extension. The mechanism by which POT1-TPP1 accomplishes these tasks is poorly understood. Read More

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Somatic deficiency causes reproductive parasitism in a fungus.

Nat Commun 2021 02 4;12(1):783. Epub 2021 Feb 4.

Laboratory of Genetics, Wageningen University, Wageningen, The Netherlands.

Some multicellular organisms can fuse because mergers potentially provide mutual benefits. However, experimental evolution in the fungus Neurospora crassa has demonstrated that free fusion of mycelia favours cheater lineages, but the mechanism and evolutionary dynamics of this exploitation are unknown. Here we show, paradoxically, that all convergently evolved cheater lineages have similar fusion deficiencies. Read More

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February 2021

Activation of meiotic recombination by nuclear import of the DNA break hotspot-determining complex in fission yeast.

J Cell Sci 2021 Feb 22;134(4). Epub 2021 Feb 22.

Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA

Meiotic recombination forms crossovers important for proper chromosome segregation and offspring viability. This complex process involves many proteins acting at each of the multiple steps of recombination. Recombination initiates by formation of DNA double-strand breaks (DSBs), which in the several species examined occur with high frequency at special sites (DSB hotspots). Read More

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February 2021