5,477 results match your criteria pas enzyme

HILIC-ESI-MS analysis of phosphatidic acid methyl esters artificially generated during lipid extraction from microgreen crops.

J Mass Spectrom 2021 Aug 28;56(10):e4784. Epub 2021 Aug 28.

Dipartimento di Chimica, Università degli Studi di Bari "Aldo Moro", Bari, Italy.

The uncontrolled activation of endogenous enzymes may introduce both qualitative and quantitative artefacts when lipids are extracted from vegetal matrices. In the present study, a method based on hydrophilic interaction liquid chromatography coupled either to high-resolution/accuracy Fourier-transform mass spectrometry (HILIC-ESI-FTMS) or to linear ion trap multiple stage mass spectrometry (HILIC-ESI-MS , with n = 2 and 3) with electrospray ionization was developed to unveil one of those artefacts. Specifically, the artificial generation of methyl esters of phosphatidic acids (MPA), catalysed by endogenous phospholipase D (PLD) during lipid extraction from five oleaginous microgreen crops (chia, soy, flax, sunflower and rapeseed), was studied. Read More

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Novel tacrine-based acetylcholinesterase inhibitors as potential agents for the treatment of Alzheimer's disease: Quinolotacrine hybrids.

Mol Divers 2021 Sep 7. Epub 2021 Sep 7.

Department of Medicinal Chemistry, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.

A new series of quinolotacrine hybrids including cyclopenta- and cyclohexa-quinolotacrine derivatives were designed, synthesized, and assessed as anti-cholinesterase (ChE) agents. The designed derivatives indicated higher inhibitory effect on the acetylcholinesterase (AChE) with IC values of 0.285-100 µM compared to butyrylcholinesterase (BChE) with IC values of > 100 µM. Read More

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September 2021

Further SAR studies on natural template based neuroprotective molecules for the treatment of Alzheimer's disease.

Bioorg Med Chem 2021 Aug 28;46:116385. Epub 2021 Aug 28.

Department of Pharmaceutical Engineering & Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India. Electronic address:

In our earlier paper, we described ferulic acid (FA) template based novel series of multifunctional cholinesterase (ChE) inhibitors for the management of AD. This report has further extended the structure-activity relationship (SAR) studies of this series of molecules in a calibrated manner to improve upon the ChEs inhibition and antioxidant property to identify the novel potent multifunctional molecules. To investigate the effect of replacement of phenylpiperazine ring with benzylpiperazine, increase in the linker length between FA and substituted phenyl ring, and replacement of indole moiety with tryptamine on this molecular template, three series of novel molecules were developed. Read More

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Endometrial glycogen metabolism on days 1 and 11 of the reproductive cycle in dairy cows.

Anim Reprod Sci 2021 Aug 20;233:106827. Epub 2021 Aug 20.

Department of Animal Science, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States. Electronic address:

Embryos need glucose or fructose to remain viable; however, it is not well understood how secretion of these carbohydrates is regulated. This study was conducted to evaluate endometrial glycogen and associated enzymes on Days 1 and 11 of the estrous cycle (Day 0 = behavioral estrus) in cattle. Diastase-liable periodic acid-Schiff (PAS) staining of luminal epithelia decreased 81 % between Days 1 and 11. Read More

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Mitochondrial Metabolism behind Region-Specific Resistance to Ischemia-Reperfusion Injury in Gerbil Hippocampus. Role of PKCβII and Phosphate-Activated Glutaminase.

Int J Mol Sci 2021 Aug 7;22(16). Epub 2021 Aug 7.

Molecular Biology Unit, Mossakowski Medical Research Institute, PAS, 02-106 Warsaw, Poland.

Ischemic episodes are a leading cause of death worldwide with limited therapeutic interventions. The current study explored mitochondrial phosphate-activated glutaminase (GLS1) activity modulation by PKCβII through GC-MS untargeted metabolomics approach. Mitochondria were used to elucidate the endogenous resistance of hippocampal CA2-4 and dentate gyrus (DG) to transient ischemia and reperfusion in a model of ischemic episode in gerbils. Read More

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Storage and Utilization of Glycogen by Mouse Liver during Adaptation to Nutritional Changes Are GLP-1 and PASK Dependent.

Nutrients 2021 Jul 26;13(8). Epub 2021 Jul 26.

Department of Biochemistry and Molecular Biology, Faculty of Medicine, Complutense University of Madrid, 28040 Madrid, Spain.

Glucagon-like peptide 1 (GLP-1) and PAS kinase (PASK) control glucose and energy homeostasis according to nutritional status. Thus, both glucose availability and GLP-1 lead to hepatic glycogen synthesis or degradation. We used a murine model to discover whether PASK mediates the effect of exendin-4 (GLP-1 analogue) in the adaptation of hepatic glycogen metabolism to nutritional status. Read More

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Synthesis, Biological Evaluation, and In Silico Studies of New Acetylcholinesterase Inhibitors Based on Quinoxaline Scaffold.

Molecules 2021 Aug 12;26(16). Epub 2021 Aug 12.

Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai 90112, Songkhla, Thailand.

A quinoxaline scaffold exhibits various bioactivities in pharmacotherapeutic interests. In this research, twelve quinoxaline derivatives were synthesized and evaluated as new acetylcholinesterase inhibitors. We found all compounds showed potent inhibitory activity against acetylcholinesterase (AChE) with IC values of 0. Read More

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Aloe vera carbohydrates regulate glucose metabolism through improved glycogen synthesis and downregulation of hepatic gluconeogenesis in diabetic rats.

J Ethnopharmacol 2021 Aug 23;281:114556. Epub 2021 Aug 23.

Vellore Institute of Technology, Vellore, Tamil Nadu, India. Electronic address:

Ethnopharmacological Relevance: Aloe vera (L.) Burm.f. Read More

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Novel chrysin derivatives as hidden multifunctional agents for anti-Alzheimer's disease: design, synthesis and in vitro evaluation.

Eur J Pharm Sci 2021 Nov 19;166:105976. Epub 2021 Aug 19.

School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China. Electronic address:

Alzheimer's disease (AD) is the most common type of dementia, the exact etiology of the disease has not been known yet. The use of single-target drugs limits the efficacy of drugs and has certain side effects. In this study, the 'hidden' multi-target strategy was used in combination with chrysin's metal chelating site and rivastigmine's anti-cholinesterase pharmacophore to form an ester, which improves the hydrophobicity and protects the phenolic hydroxyl group at the same time. Read More

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November 2021

Molecularly imprinted polymer nanoparticles-based electrochemical chemosensors for selective determination of cilostazol and its pharmacologically active primary metabolite in human plasma.

Biosens Bioelectron 2021 Aug 5;193:113542. Epub 2021 Aug 5.

Institute of Physical Chemistry, Polish Academy of Sciences (IPC PAS), Kasprzaka 44/52, 01-224, Warsaw, Poland; Faculty of Mathematics and Natural Sciences. School of Sciences, Cardinal Stefan Wyszynski University in Warsaw, Woycickiego 1/3, 01-815, Warsaw, Poland. Electronic address:

Molecularly imprinted polymer (MIP) nanoparticles-based differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) chemosensors for antiplatelet drug substance, cilostazol (CIL), and its pharmacologically active primary metabolite, 3,4-dehydrocilostazol (dhCIL), selective determination in human plasma were devised, prepared, and tested. Molecular mechanics (MM), molecular dynamics (MD), and density functional theory (DFT) simulations provided the optimum structure and predicted the stability of the pre-polymerization complex of the CIL template with the chosen functional acrylic monomers. Moreover, they accounted for the MIP selectivity manifested by the molecularly imprinted cavity with the CIL molecule complex stability higher than that for each interference. Read More

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The SAC1 phosphatase domain of synaptojanin-1 is activated by interacting with polyunsaturated fatty acid-containing phosphatidic acids.

FEBS Lett 2021 Aug 13. Epub 2021 Aug 13.

Department of Chemistry, Graduate School of Science, Chiba University, Japan.

Although there are many phosphatidic acid (PA) molecular species based on its fatty acyl compositions, their interacting partners have been poorly investigated. Here, we identified synaptojanin-1 (SYNJ1), Parkinson's disease-related protein that is essential for regulating clathrin-mediated synaptic vesicle endocytosis via dually dephosphorylating D5 and D4 position phosphates from phosphatidylinositol (PI) (4,5)-bisphosphate, as a 1-stearoyl-2-docosahexaenoyl (18:0/22:6)-PA-binding protein. SYNJ1 failed to substantially associate with other acidic phospholipids. Read More

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A comparison of detection methods of mesocercariae in wild boar () meat.

Int J Parasitol Parasites Wildl 2021 Dec 18;16:1-4. Epub 2021 Jul 18.

Department of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776, Warsaw, Poland.

(DMS), the mesocercariae of , is typically found accidently during examination of wild boar meat for spp. The aim of the study was to compare DMS detection methods. Briefly, 232 wild boar meat samples were tested by mesocercariae migration technique (AMT) as a reference method; of these, 104 were found to be positive. Read More

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December 2021

Study effect of Baicalein encapsulated/loaded Chitosan-nanoparticle on allergic Asthma pathology in mouse model.

Saudi J Biol Sci 2021 Aug 20;28(8):4311-4317. Epub 2021 Apr 20.

Department of Immunology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.

Asthma as chronic airway disease has high prevalence in children and imbalance of Th1/Th2 is a critical mechanism in pathogenesis of the asthma. Baicalein as a cell protective and anti-inflammatory flavonoid may have anti-asthma effect. Therefore, for better using lung, baicalein was used in chitosan-nanoparticle as anti-asthma treatment. Read More

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LC-HRMS/MS phytochemical profiling of Symphytum officinale L. and Anchusa ochroleuca M. Bieb. (Boraginaceae): Unveiling their multi-biological potential via an integrated approach.

J Pharm Biomed Anal 2021 Sep 24;204:114283. Epub 2021 Jul 24.

Biothermodynamics, TUM School of Life Sciences, Technical University of Munich, 85354, Freising, Germany. Electronic address:

This study was aimed at providing a comprehensive phytochemical characterization and multi-biological assessment of Symphytum officinale L., a medicinal plant with a noteworthy traditional use, and Anchusa ochroleuca M. Bieb. Read More

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September 2021

[Rutin activates AMPKα to ameliorate liver damage caused by perfluorooctanoic acid in mice].

Wei Sheng Yan Jiu 2021 Jul;50(4):609-614

School of Public Health, Bengbu Medical College, Anhui Province Key Laboratory of Immunology in Chronic Diseases, Bengbu 233030, China.

Objective: To study the protective effect and mechanism of rutin on perfluorooctanoic acid(PFOA)-induced liver damage in mice.

Methods: A total of 24 male ICR mice were randomly divided into 3 groups, 8 in each group: control group(ctrl group: ddH_2O), perfluorooctanoic acid group(PFOA group: PFOA 20 mg/(kg·d)), rutin intervention group(rutin+PFOA group: PFOA 20 mg/(kg·d)+rutin 20 mg/(kg·d)), normal diet, oral gavage, daily observation, weighting, and recording. After 14 days of treatment, the liver was quickly stripped and weighted after blood sampling and execution. Read More

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A pilot study of spray cryotherapy effects on airway secretions.

Cryobiology 2021 Jul 24. Epub 2021 Jul 24.

Department of Respiratory Medicine, Shanghai 10th People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China. Electronic address:

Spray cryotherapy (SCT) is a new transbronchial approach that disrupts epithelial cells by freezing. However, there are limited data addressing the effect of SCT on airway secretion. The aim of this study was to evaluate if SCT effect on airway secretion and the possible mechanism in canines. Read More

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The protective effect of NF-κB signaling pathway inhibitor PDTC on mice with chronic atrophic gastritis.

Scand J Gastroenterol 2021 Oct 26;56(10):1131-1139. Epub 2021 Jul 26.

Outpatient Department, Chongqing University Central Hospital (Chongqing Emergency Medical Center), Chongqing, China.

Objective: To understand the protective effect of NF-κB signaling pathway inhibitor pyrrolidinedithiocarbamate (PDTC) on mice with chronic atrophic gastritis (CAG).

Methods: () infection combined with high-salt diet was used to construct the CAG mouse model, and 100 or 200 mg/kg/day PDTC was intragastrically treated for 8 weeks. Then, hematoxylin and eosin (HE) and Alcian blue-periodic acid-Schiff (AB-PAS) staining were used to observe the pathology of gastric mucosa, while immunohistochemistry, quantitative real-time polymerase chain reaction (qRT-PCR), enzyme-linked immuno sorbent assay (ELISA) and western blotting were determined to detect the expression of related molecules. Read More

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October 2021

The G-Protein-Coupled Membrane Estrogen Receptor Is Present in Horse Cryptorchid Testes and Mediates Downstream Pathways.

Int J Mol Sci 2021 Jul 1;22(13). Epub 2021 Jul 1.

University Centre of Veterinary Medicine JU-UA, University of Agriculture in Krakow, Mickiewicza 24/28, 30-059 Krakow, Poland.

Cryptorchidism in horses is a commonly occurring malformation. The molecular basis of this pathology is not fully known. In addition, the origins of high intratesticular estrogen levels in horses remain obscure. Read More

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Case report: Gaucher disease in trepanobiopsy of 16yo woman examined for suspected myelodysplastic syndrome.

Cesk Patol 2021 ;57(2):105-108

Gaucher disease is an autosomal recessive disease belonging to the so-called storage diseases. More than 300 mutations of the GBA1 gene encoding the β-glucocerebrosidase enzyme are known. It is a very rare disease in the Czech Republic. Read More

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Photoreaction of photoactivated adenylate cyclase from cyanobacterium Microcoleus chthonoplastes.

J Photochem Photobiol B 2021 Aug 6;221:112252. Epub 2021 Jul 6.

Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan. Electronic address:

The photochemical reaction of photoactivated adenylate cyclase from cyanobacterium Microcoleus chthonoplastes PCC 7420 (mPAC), which consists of a Per-Arnt-Sim (PAS), a light‑oxygene-voltage (LOV), and an adenylate cyclase (AC) domain, was investigated mainly using the time-resolved transient grating method. An absorption spectral change associated with an adduct formation between its chromophore (flavin mononucleotide) and a cysteine residue was observed with a time constant of 0.66 μs. Read More

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Host-emitted amino acid cues regulate bacterial chemokinesis to enhance colonization.

Cell Host Microbe 2021 Aug 6;29(8):1221-1234.e8. Epub 2021 Jul 6.

Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA; Humans and the Microbiome Program, CIFAR, Toronto, ON, Canada. Electronic address:

Animal microbiomes are assembled predominantly from environmental microbes, yet the mechanisms by which individual symbionts regulate their transmission into hosts remain underexplored. By tracking the experimental evolution of Aeromonas veronii in gnotobiotic zebrafish, we identify bacterial traits promoting host colonization. Multiple independently evolved isolates with increased immigration harbored mutations in a gene we named sensor of proline diguanylate cyclase enzyme (SpdE) based on structural, biochemical, and phenotypic evidence that SpdE encodes an amino-acid-sensing diguanylate cyclase. Read More

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[Preliminary study of the changes in blood system in pyrrolizidine alkaloid-related liver damage].

Zhonghua Gan Zang Bing Za Zhi 2021 Jun;29(6):533-538

Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai 200032, China Evidence-based Medicine Center of Fudan University, Shanghai 200032, China.

To preliminary explore the changes in blood system in pyrrolizidine alkaloids (PAs)-related liver damage. General situation, liver function, biochemical blood test, routine blood test, coagulation function markers, etc., of 77 cases with drug-induced liver damage admitted to the Zhongshan Hospital Affiliated to Fudan University from 2012 to 2019 were retrospectively analyzed. Read More

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CircRNA circ-ITCH improves renal inflammation and fibrosis in streptozotocin-induced diabetic mice by regulating the miR-33a-5p/SIRT6 axis.

Inflamm Res 2021 Jul 3;70(7):835-846. Epub 2021 Jul 3.

Department of Nephrology, Jiangsu Province Geriatric Hospital, Geriatric Hospital of Nanjing Medical University, Jiangsu Province Official Hospital, 65 Jiangsu road, Nanjing, 210024, Jiangsu province, China.

Diabetic nephropathy (DN) seriously affects the people's life and health in China. This study aimed to investigate the effect of circRNA circ-ITCH on improving DN by regulating the miR-33a-5p/SIRT6 axis and the possible mechanism of action. High glucose (HG)-induced rat mesangial cells (RMCs) were used to simulate the DN in vitro. Read More

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Antioxidant, Anti-Inflammatory, and Anti-Diabetic Activity of Phenolic Acids Fractions Obtained from (L.) Juss.

Molecules 2021 Jun 8;26(12). Epub 2021 Jun 8.

Chair and Department of Pharmaceutical Botany, Medical University, 1 Chodźki Street, 20-093 Lublin, Poland.

Many plants that are commonly used in folk medicine have multidirectional biological properties confirmed by scientific research. One of them is (L.) Juss. Read More

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Juvenile and adult vulvar pemphigoid, an under recognized entity: Case series of fourteen patients.

JAAD Case Rep 2021 Jul 24;13:75-80. Epub 2021 May 24.

Department of Dermatology, Center of Blistering Diseases, European Reference Network-Skin Member, University Medical Center Groningen, University of Groningen, Groningen, Netherlands.

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Ectopic Expression of Enhanced Salt Tolerance Due to Accumulated Spd Content and Activation of Salt Tolerance-Related Genes in .

DNA Cell Biol 2021 Sep 24;40(9):1144-1157. Epub 2021 Jun 24.

College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang, China.

Polyamines (PAs), especially spermidine and spermine (which are involved in various types of abiotic stress tolerance), have been reported in many plant species. In this study, we identified 14 putative S-adenosylmethionine decarboxylase genes (-) in upland cotton. Based on phylogenetic and expression analyses conducted under different abiotic stresses, we selected and transferred into . Read More

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September 2021

Oestrogen receptor activity in hormone-dependent breast cancer during chemotherapy.

EBioMedicine 2021 Jul 20;69:103451. Epub 2021 Jun 20.

Translational Genomics and Targeted Therapies in Solid Tumors, August Pi i Sunyer Biomedical Research Institute (IDIBAPS), Barcelona, Spain; Department of Medical Oncology, Hospital Clinic of Barcelona, Spain; SOLTI cooperative group, Barcelona, Spain; Institute of Oncology (IOB)-Hospital Quirónsalud, Barcelona, Spain; Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, USA; Department of Medicine, University of Barcelona, Barcelona, Spain. Electronic address:

Background: Chemotherapy efficacy in early-stage hormone receptor-positive (HR+) breast cancer (BC) according to menopausal status needs a biological explanation.

Methods: We compared early-stage HR+ BC biological features before and after (neo)adjuvant chemotherapy or endocrine therapy (ET), and assessed oestrogen receptor (ER) pathway activity in both pre- and post-menopausal patients. The nCounter platform was used to detect gene expression levels. Read More

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Catalytic activity and stereoselectivity of engineered phosphotriesterases towards structurally different nerve agents in vitro.

Arch Toxicol 2021 08 23;95(8):2815-2823. Epub 2021 Jun 23.

Institut für Pharmakologie und Toxikologie der Bundeswehr, 80937, Munich, Germany.

Highly toxic organophosphorus nerve agents, especially the extremely stable and persistent V-type agents such as VX, still pose a threat to the human population and require effective medical countermeasures. Engineered mutants of the Brevundimonas diminuta phosphotriesterase (BdPTE) exhibit enhanced catalytic activities and have demonstrated detoxification in animal models, however, substrate specificity and fast plasma clearance limit their medical applicability. To allow better assessment of their substrate profiles, we have thoroughly investigated the catalytic efficacies of five BdPTE mutants with 17 different nerve agents using an AChE inhibition assay. Read More

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Gastroprotective Effect of Ethanol Extracts from Bark of Magnolia officinalis on Ethanol-Induced Gastric Mucosal Damage in Rats.

Biomed Res Int 2021 31;2021:6688414. Epub 2021 May 31.

Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.

Rehd. and Wils. is widely used in Asian countries because of its multiple pharmacological effects. Read More

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Metabolic Syndrome Mediates ROS-miR-193b-NFYA-Dependent Downregulation of Soluble Guanylate Cyclase and Contributes to Exercise-Induced Pulmonary Hypertension in Heart Failure With Preserved Ejection Fraction.

Circulation 2021 Aug 23;144(8):615-637. Epub 2021 Jun 23.

Pittsburgh Heart, Lung and Blood Vascular Medicine Institute (T.S., L.W., C.E.-D., S.A.H., E.R.R., M.R.D., J.J.B., Y.T., M.R., S.S., C.S.H., D.G., D.A.G., E.A.G., S.Y.C., A.C.S., C.F.M., M.T.G.), University of Pittsburgh School of Medicine, PA.

Background: Many patients with heart failure with preserved ejection fraction have metabolic syndrome and develop exercise-induced pulmonary hypertension (EIPH). Increases in pulmonary vascular resistance in patients with heart failure with preserved ejection fraction portend a poor prognosis; this phenotype is referred to as combined precapillary and postcapillary pulmonary hypertension (CpcPH). Therapeutic trials for EIPH and CpcPH have been disappointing, suggesting the need for strategies that target upstream mechanisms of disease. Read More

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