804 results match your criteria p53-dependent bcl-2


The role of P53 up-regulated modulator of apoptosis (PUMA) in ovarian development, cardiovascular and neurodegenerative diseases.

Authors:
Mei Li

Apoptosis 2021 Mar 30. Epub 2021 Mar 30.

Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.

P53 up-regulated modulator of apoptosis (PUMA), a pro-apoptotic BCL-2 homology 3 (BH3)-only member of the BCL-2 family, is a direct transcriptional target of P53 that elicits mitochondrial apoptosis under treatment with radiation and chemotherapy. It also induces excessive apoptosis in cardiovascular and/or neurodegenerative diseases. PUMA has been found to play a critical role in ovarian apoptosis. Read More

View Article and Full-Text PDF

p53 induces ARTS to promote mitochondrial apoptosis.

Cell Death Dis 2021 Feb 24;12(2):204. Epub 2021 Feb 24.

Fudan University Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China.

Apoptosis related protein in TGF-β signaling pathway (ARTS) was originally discovered in cells undergoing apoptosis in response to TGF-β, but ARTS also acts downstream of many other apoptotic stimuli. ARTS induces apoptosis by antagonizing the anti-apoptotic proteins XIAP and Bcl-2. Here we identified the pro-apoptotic Sept4/ARTS gene as a p53-responsive target gene. Read More

View Article and Full-Text PDF
February 2021

Attenuating role of withaferin A in the proliferation and migration of lung cancer cells via a p53-miR-27a/miR-10b pathway.

Oncol Lett 2021 Mar 26;21(3):232. Epub 2021 Jan 26.

Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan, R.O.C.

MicroRNAs (miRNAs/miRs) are known to play a key role in tumorigenesis and usually serve as therapeutic targets in cancer treatment. In the present study, the inhibitory effects and the targeting miRNAs of withaferin A (WA) were investigated in human lung cancer cells. Different lung cancer cell lines were administrated with different concentrations of WA for different time interval followed by western blot or reverse transcription-quantitative PCR analyses to determine the underlying signaling pathway. Read More

View Article and Full-Text PDF

Cyclopiazonic acid induced p53-dependent apoptosis in the testis of mice: Another male related risk factor of infertility.

Environ Toxicol 2021 May 5;36(5):903-913. Epub 2021 Jan 5.

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran.

Cyclopiazonic acid (CPA) is an indole tetrameric acid mycotoxin. This study carried out to investigate the potential effects of CPA on male reproductive system. In the current study, 40 adult male mice were divided into five groups (n = 8). Read More

View Article and Full-Text PDF

Diclofenac Sodium Triggers p53-Dependent Apoptosis in Human Corneal Epithelial Cells via ROS-Mediated Crosstalk.

Chem Res Toxicol 2021 Jan 28;34(1):70-79. Epub 2020 Dec 28.

Laboratory for Corneal Tissue Engineering, College of Marine Life Sciences, Ocean University of China, Qingdao, Shandong 266100, China.

Diclofenac sodium (DFS), a nonsteroidal anti-inflammatory drug, is frequently used in ophthalmology, but it causes negative effects on corneas. The mechanisms underlying the toxicities to corneas remains unclear. The present study was designed to assess the cytotoxicity of DFS to human corneal epithelial (HCEP) cells and further investigate its related mechanisms. Read More

View Article and Full-Text PDF
January 2021

Triggered ROS-Induced DNA Damage to Arrest Cell Cycle Progression and Induce Apoptosis on Human Hepatocellular Carcinoma In Vitro and In Vivo.

Molecules 2020 Nov 30;25(23). Epub 2020 Nov 30.

Department of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung 40201, Taiwan.

The purpose of the study was to elucidate the anti-hepatoma effects and mechanisms of essential oils (PPa extract) in vitro and in vivo. PPa extract exhibited an inhibitory effect on hepatocellular carcinoma (HCC) cells and was less cytotoxic to normal cells, especially normal liver cells, than it was to HCC cells, exerting a good selective index. Additionally, PPa extract inhibited HCC cell growth by blocking the cell cycle at the G/G phase via p53 dependent or independent pathway to down regulated cell cycle regulators. Read More

View Article and Full-Text PDF
November 2020

Amiodarone's major metabolite, desethylamiodarone inhibits proliferation of B16-F10 melanoma cells and limits lung metastasis formation in an in vivo experimental model.

PLoS One 2020 25;15(9):e0239088. Epub 2020 Sep 25.

Department of Biochemistry and Medical Chemistry, University of Pecs, Medical School, Pecs, Hungary.

Previously, we demonstrated the in vitro anti-tumor effects of desethylamiodarone (DEA) in bladder and cervix cancer cell lines. In the present study, we intended to establish its potentiality in B16-F10 metastatic melanoma cells in vitro and in vivo. We assessed cell proliferation, apoptosis and cell cycle by using sulforhodamine B assay, Muse™ Annexin V & Dead Cell and Muse® Cell Cycle assays, respectively. Read More

View Article and Full-Text PDF
November 2020

NT5DC2 suppression restrains progression towards metastasis of non-small-cell lung cancer through regulation p53 signaling.

Biochem Biophys Res Commun 2020 12 19;533(3):354-361. Epub 2020 Sep 19.

Department of Respiration, The First Hospital of Jilin University, Changchun, 130021, China. Electronic address:

Non-small cell lung cancer (NSCLC) is a leading cause of tumor mortality worldwide. Nevertheless, the molecular mechanisms revealing NSCLC progression are still unclear. 5'-Nucleotidase domain containing 2 (NT5DC2), as a member of the NT5DC family, contains a haloacid dehalogenase motif localized in the N-terminus of these proteins. Read More

View Article and Full-Text PDF
December 2020

Crosstalk between Noxa, Bcl-2, and ceramide in mediating p53-dependent apoptosis in Molt-4 human T-cell leukemia.

Mol Cell Biochem 2020 Dec 7;475(1-2):215-226. Epub 2020 Aug 7.

Department of Biochemistry and Molecular Genetics, Faculty of Medicine, American University of Beirut, Beirut, Lebanon.

Ionizing radiation induces apoptosis in human Molt-4 leukemia cells in a p53-dependent manner. The tumor suppressor p53 stimulates various downstream targets that presumably trigger, individually or in concert, de novo ceramide synthesis and intrinsic apoptosis via mitochondrial outer membrane permeabilization (MOMP). Among these targets, BH3-only protein Noxa was found to be promptly activated by p53 prior to ceramide accumulation and apoptosis in response to irradiation. Read More

View Article and Full-Text PDF
December 2020

Elephantopus mollis Kunth extracts induce antiproliferation and apoptosis in human lung cancer and myeloid leukemia cells.

J Ethnopharmacol 2020 Dec 5;263:113222. Epub 2020 Aug 5.

Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu, Ho Chi Minh City, 700000, Viet Nam; Laboratory of Molecular Biotechnology, VNU-HCM, University of Science, 227 Nguyen Van Cu, Ho Chi Minh City, 700000, Viet Nam; Laboratory of Cancer Research, VNU-HCM, University of Science, 227 Nguyen Van Cu, Ho Chi Minh City, 700000, Viet Nam; Vietnam National University, Ho Chi Minh City, Viet Nam. Electronic address:

Ethnopharmacological Relevance: Elephantopus mollis Kunth (EM), which belongs to Asteraceae family, has been used as a folk medicine with diverse therapeutic properties. Previous studies reported that crude extracts of this plant could inhibit several cancer cell lines, including breast carcinoma MCF-7, liver carcinoma HepG2, colorectal carcinoma DLD-1, lung carcinoma NCI-H23, etc. AIM: In this study, the anticancer activity and associated molecular mechanism of EM which is distributed in Vietnam were investigated. Read More

View Article and Full-Text PDF
December 2020

Melatonin Attenuates Thrombin-induced Inflammation in BV2 Cells and Then Protects HT22 Cells from Apoptosis.

Inflammation 2020 Oct;43(5):1959-1970

Department of Neurology, Yan Cheng City No.1 People's Hospital, Yancheng, Jiangsu Province, China.

Increasing evidence has revealed that the uncontrolled thrombin-induced inflammation following intracerebral hemorrhage (ICH) plays a key role in ICH. Oxidative stress and neuroinflammatory responses are interdependent and bidirectional events. Melatonin is now recognized as an antioxidant and a free radical scavenger due to its roles in various physiological and pathological processes. Read More

View Article and Full-Text PDF
October 2020

Chemical, Physical and Biological Triggers of Evolutionary Conserved Bcl-xL-Mediated Apoptosis.

Cancers (Basel) 2020 Jun 25;12(6). Epub 2020 Jun 25.

Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, 7028 Trondheim, Norway.

Background: The evidence that pan-Bcl-2 or Bcl-xL-specific inhibitors prematurely kill virus-infected or RNA/DNA-transfected cells provides rationale for investigating these apoptotic inducers further. We hypothesized that not only invasive RNA or DNA (biological factors) but also DNA/RNA-damaging chemical or physical factors could trigger apoptosis that have been sensitized with pan-Bcl-2 or Bcl-xL-specific agents; Methods: We tested chemical and physical factors plus Bcl-xL-specific inhibitor A-1155463 in cells of various origins and the small roundworms (); Results: We show that combination of a A-1155463 along with a DNA-damaging agent, 4-nitroquinoline-1-oxide (4NQO), prematurely kills cells of various origins as well as . The synergistic effect is p53-dependent and associated with the release of Bad and Bax from Bcl-xL, which trigger mitochondrial outer membrane permeabilization. Read More

View Article and Full-Text PDF

Purified Tea ( (L.) ) Flower Saponins Induce the p53-Dependent Intrinsic Apoptosis of Cisplatin-Resistant Ovarian Cancer Cells.

Int J Mol Sci 2020 Jun 17;21(12). Epub 2020 Jun 17.

Department of Tea Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.

Ovarian cancer is currently ranked at fifth in cancer deaths among women. Patients who have undergone cisplatin-based chemotherapy can experience adverse effects or become resistant to treatment, which is a major impediment for ovarian cancer treatment. Natural products from plants have drawn great attention in the fight against cancer recently. Read More

View Article and Full-Text PDF

Effect of epiberberine from Coptis chinensis Franch on inhibition of tumor growth in MKN-45 xenograft mice.

Phytomedicine 2020 May 17;76:153216. Epub 2020 May 17.

Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing 400715, China. Electronic address:

Background And Purpose: Gastric cancer is one of the major malignancies worldwide. Epiberberine (EPI) is a major alkaloid from Coptis chinensis Franch and the antitumor property of EPI remains poorly understood.

Method: The inhibition on gastric cancer cells was observed by MTT assays and colony formation experiments. Read More

View Article and Full-Text PDF

Down-Regulation of miR-23a-3p Mediates Irradiation-Induced Neuronal Apoptosis.

Int J Mol Sci 2020 May 24;21(10). Epub 2020 May 24.

Center for Shock Trauma Anesthesiology Research, Department of Anesthesiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

Radiation-induced central nervous system toxicity is a significant risk factor for patients receiving cancer radiotherapy. Surprisingly, the mechanisms responsible for the DNA damage-triggered neuronal cell death following irradiation have yet to be deciphered. Using primary cortical neuronal cultures in vitro, we demonstrated that X-ray exposure induces the mitochondrial pathway of intrinsic apoptosis and that miR-23a-3p plays a significant role in the regulation of this process. Read More

View Article and Full-Text PDF

Cytosolic and mitochondrial ROS production resulted in apoptosis induction in breast cancer cells treated with Crocin: The role of FOXO3a, PTEN and AKT signaling.

Biochem Pharmacol 2020 07 28;177:113999. Epub 2020 Apr 28.

Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box: 14155-331, Tehran, Iran. Electronic address:

Different groups have reported the Crocin anticancer activity. We previously showed Crocin-induced apoptosis in rat model of breast and gastric cancers, through the increased Bax/Bcl-2 ratio and caspases activity, as well as the cell cycle arrest in a p53-dependent manner. Since Crocin antioxidant activity has been shown under different conditions, it is interesting to elucidate its apoptotic mechanism. Read More

View Article and Full-Text PDF

Involvement of p53-dependent apoptosis signal in antitumor effect of Colchicine on human papilloma virus (HPV)-positive human cervical cancer cells.

Biosci Rep 2020 03;40(3)

Department of Gynecology, Guangdong Women and Children Hospital, Guangzhou 511442, P.R. China.

Colchicine, a plant-derived alkaloid with relatively low toxicity on normal human epidermal keratinocytes (HEKn), has selective inhibitory effect on the growth of CaSki (HPV16-positive) and HeLa (HPV18-positive) human cervical cancer cell lines via the induction of apoptosis. Colchicine (2.5, 5. Read More

View Article and Full-Text PDF

Helveticoside Exhibited p53-dependent Anticancer Activity Against Colorectal Cancer.

Arch Med Res 2020 04 6;51(3):224-232. Epub 2020 Mar 6.

College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, Henan, China.

Background: Investigation into the anti-cancer activities of natural products and their derivatives represents an efficient approach to develop safe and effective chemotherapeutic agents for the treatment of colorectal cancer. Helveticoside is a biologically active component of the seed extract of Descurainia sophia. This compound has been reported to regulate the genes related to cell proliferation and apoptosis in lung cancer cells, however its anticancer activity has not been fully explored yet. Read More

View Article and Full-Text PDF

Application of non-invasive low-intensity pulsed electric field with thermal cycling-hyperthermia for synergistically enhanced anticancer effect of chlorogenic acid on PANC-1 cells.

PLoS One 2020 29;15(1):e0222126. Epub 2020 Jan 29.

Department of Physics, Lab for Medical Physics & Biomedical Engineering, National Taiwan University, Taipei, Taiwan.

Most existing cancer treatments involve high-cost chemotherapy and radiotherapy, with major side effects, prompting effort to develop alternative treatment modalities. It was reported that the combination of thermal-cycling hyperthermia (TC-HT) and phenolic compound exhibited a moderate cytotoxic effect against human pancreatic cancer PANC-1 cells. In this study, we investigate the efficacy of triple combination in PANC-1 cancer cells by adopting low-intensity pulsed electric field (LIPEF) to couple with TC-HT and CGA (chlorogenic acid). Read More

View Article and Full-Text PDF

Tetra-cationic platinum(II) porphyrins like a candidate photosensitizers to bind, selective and drug delivery for metastatic melanoma.

J Photochem Photobiol B 2020 Jan 26;202:111725. Epub 2019 Nov 26.

Molecular and Cellular Oncology Research Group, Cancer Biotechnology Laboratory, Technological Development Center, Federal University of Pelotas, Pelotas, Brazil. Electronic address:

Photodynamic therapy (PDT) is an expanding treatment modality due to its minimally invasive localized activity and few adverse effects. This therapy requires photosensitive compounds, which have high sensitivity to light exposure. Thus, in this work, the in vitro antitumor activity of meso-tetra(3- and 4-pyridyl)porphyrins (3-TPyP and 4-TPyP) in metastatic melanoma cell (WM1366 line) and non-tumoral Ovarian lineage Chinese Hamister (CHO) was evaluated using photodynamic process. Read More

View Article and Full-Text PDF
January 2020

Mutant p53 drives cancer chemotherapy resistance due to loss of function on activating transcription of PUMA.

Cell Cycle 2019 Dec 14;18(24):3442-3455. Epub 2019 Nov 14.

College of Biology, Hunan University, Changsha, China.

is a critical tumor suppressor gene, activating p53 and its downstream targets to induce apoptosis is a promising way for cancer therapy. However, more than 50% of cancer patients have p53 mutations, which may cause cancer therapy resistance, and the underline mechanism is poorly understood. Here, we found that cell viability decrease and apoptosis induced by p53-dependent traditional drugs in colon cancer cells were eliminated in p53 mutant cells. Read More

View Article and Full-Text PDF
December 2019

A small cytotoxic peptide from frog elicits potent antitumor immunity to prevent local tumor growth and metastases.

Future Med Chem 2019 10;11(19):2505-2525

School of bioengineering, Dalian University of Technology, Dalian, Liaoning 116024, China.

Anticancer immunochemotherapy represents an attractive paradigm to improve therapeutic responses and reduce side effects. Here, we show that a naturally occurring host defense peptide, HN-1 inhibited multiple malignant cells proliferation and tumor growth in a xenografted human breast tumor model. Acting through MAPK/NF-κB pathways, HN-1 induced a caspase-independent mitochondrial apoptosis, as indicated by a p53-dependent increase of Bax/Bcl-2 ratio and the nuclear translocation of apoptosis inducing factor. Read More

View Article and Full-Text PDF
October 2019

Actinomycin V Suppresses Human Non-Small-Cell Lung Carcinoma A549 Cells by Inducing G2/M Phase Arrest and Apoptosis via the p53-Dependent Pathway.

Mar Drugs 2019 Oct 9;17(10). Epub 2019 Oct 9.

College of Marine Science, Shandong University, Weihai 264209, China.

Actinomycin V, extracted and separated from marine-derived actinomycete , as the superior potential replacement of actinomycin D (which showed defect for its hepatotoxicity) has revealed an ideal effect in the suppression of migration and invasion in human breast cancer cells as referred to in our previous study. In this study, the involvement of p53 in the cell cycle arrest and pro-apoptotic action of actinomycin V was investigated in human non-small-cell lung carcinoma A549 cells. Results from the 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide assay showed that cytotoxic activity of actinomycin V on A549 cells (with wild-type p53) was stronger than the NCI-H1299 cells (p53-deficient). Read More

View Article and Full-Text PDF
October 2019

Melatonin Attenuates AlCl-Induced Apoptosis and Osteoblastic Differentiation Suppression by Inhibiting Oxidative Stress in MC3T3-E1 Cells.

Biol Trace Elem Res 2020 Jul 9;196(1):214-222. Epub 2019 Sep 9.

Northeastern Science Inspection Station, China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, College of Veterinary Medicine, Northeast Agricultural University, NO. 600 Changjiang Street, Xiangfang District, Harbin, 150030, China.

Aluminum (Al) inhibits osteoblast-mediated bone formation by oxidative stress, resulting in Al-induced bone disease. Melatonin (MT) has received extensive attention due to its antioxidant and maintenance of bone health effect. To evaluate the protective effect and mechanism of MT on AlCl-induced osteoblast dysfunction, MC3T3-E1 cells were treated with MT (100 μM) and/or AlCl (8 μM). Read More

View Article and Full-Text PDF

Recombinant Pierisin-5 Induces Apoptosis and Differential Expression of Bcl-2, Bax, and p53 in Human Cancer Cells.

DNA Cell Biol 2019 Aug 24;38(8):773-785. Epub 2019 Jul 24.

1Department of Biotechnology, Mizoram University, Aizawl, India.

Pierisin-5 protein (pie-5) belongs to a family of proteins possessing DNA-dependent ADP-ribosyltransferase activity, which can induce apoptotic cell death. The baculovirus-mediated expression vector system (BEVS) has been commonly used for expression of heterologous protein subunits for basic scientific research, in addition to the development and production of diagnostics and vaccines. In this study, a new method for the expression of the cytotoxic protein was established using the baculovirus expression system. Read More

View Article and Full-Text PDF

p53-dependent upregulation of miR-16-2 by sanguinarine induces cell cycle arrest and apoptosis in hepatocellular carcinoma.

Cancer Lett 2019 09 1;459:50-58. Epub 2019 Jun 1.

Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi, 710071, China. Electronic address:

MicroRNAs (miRNAs) were involved in cancer progression, and the targeting of miRNAs by natural agents has opened avenues for cancer treatment and drug development. miR-16 functions as a tumor suppressor and is frequently deleted or downregulated in various human cancers, including hepatocellular carcinoma (HCC). In the present study, we employed a miR-16-responsive luciferase reporter to screen candidate compounds that modulate miR-16 expression from a natural product library. Read More

View Article and Full-Text PDF
September 2019

Inotodiol inhibits cells migration and invasion and induces apoptosis via p53-dependent pathway in HeLa cells.

Phytomedicine 2019 Jul 13;60:152957. Epub 2019 May 13.

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China; College of Forestry, Beijing Forestry University, Beijing 100083, China. Electronic address:

Background: Inonotus obliquus, namely as Chaga mushroom, is a medicinal and edible fungus, which is widely used in food and medical fields. Inotodiol, a natural lanostane-type triterpenoid with remarkable pharmacological activities, was isolated from Inonotus obliquus, which its potential anti-tumor molecular mechanism was elaborated poorly.

Purpose: The aim of the present study was to investigate the effect of Inotodiol on HeLa cell migration, invasion and apoptosis through p53-dependent pathway. Read More

View Article and Full-Text PDF

Lumichrome Inhibits Human Lung Cancer Cell Growth and Induces Apoptosis via a p53-Dependent Mechanism.

Nutr Cancer 2019 10;71(8):1390-1402. Epub 2019 May 10.

Cell-based Drug and Health Products Development Research Unit, Chulalongkorn University , Bangkok , Thailand.

Lumichrome, a major derivative of riboflavin, may exhibit pharmacological activity against cancer cells. Riboflavin is a vitamin found in food, however, certain evidence has suggested its possible potentiating effects on cancer progression. Here, we have shown for the first time that unlike riboflavin, lumichrome can suppress lung cancer cell growth and reduce survival in both normal and anchorage-independent conditions. Read More

View Article and Full-Text PDF

Daucosterol disturbs redox homeostasis and elicits oxidative-stress mediated apoptosis in A549 cells via targeting thioredoxin reductase by a p53 dependent mechanism.

Eur J Pharmacol 2019 Jul 3;855:112-123. Epub 2019 May 3.

Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, 630 003, Tamil Nadu, India. Electronic address:

Daucosterol (DS) is a plant phytosterol which is shown to induce oxidative stress mediated apoptosis in various cancer cell lines. However, the molecular mechanism underlying its cellular action has not been documented against Non- Small Cell Lung Cancer (NSCLC). Therefore, we attempted to decipher the mechanisms responsible for DS-induced anti-proliferation on human NSCLC cells. Read More

View Article and Full-Text PDF

Induction of Apoptosis by a Combination of 2-Deoxyglucose and Metformin in Esophageal Squamous Cell Carcinoma by Targeting Cancer Cell Metabolism.

Iran J Med Sci 2019 Mar;44(2):99-107

Department of Statistics and Epidemiology, Tabriz University of Medical Sciences, Tabriz, Iran.

Background: Both mitochondrial dysfunction and aerobic glycolysis are signs of growing aggressive cancer. If altered metabolism of cancer cell is intended, using the glycolysis inhibitor (2-deoxyglucose (2DG)) would be a viable therapeutic method. The AMP-activated protein kinase (AMPK), as a metabolic sensor, could be activated with metformin and it can also launch a p53-dependent metabolic checkpoint and might inhibit cancer cell growth. Read More

View Article and Full-Text PDF