40,004 results match your criteria oxidative phosphorylation

Loss of LUC7L2 and U1 snRNP subunits shifts energy metabolism from glycolysis to OXPHOS.

Mol Cell 2021 Apr 10. Epub 2021 Apr 10.

Department of Molecular Biology and Howard Hughes Medical Institute, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. Electronic address:

Oxidative phosphorylation (OXPHOS) and glycolysis are the two major pathways for ATP production. The reliance on each varies across tissues and cell states, and can influence susceptibility to disease. At present, the full set of molecular mechanisms governing the relative expression and balance of these two pathways is unknown. Read More

View Article and Full-Text PDF

A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.

Cell Rep 2021 Apr;35(2):108963

Key Laboratory of Laboratory Medicine, Ministry of Education, Zhejiang Provincial Key Laboratory of Medical Genetics, Department of Cell Biology and Medical Genetics, College of Laboratory Medicine and Life sciences, Wenzhou Medical University, Wenzhou 325000, China; Department of Laboratory Medicine, Zhejiang Provincial People's Hospital, Affiliated People's Hospital of Hangzhou Medical College, Hangzhou 310000, China. Electronic address:

The assembly pathways of mitochondrial respirasome (supercomplex I+III+IV) are not fully understood. Here, we show that an early sub-complex I assembly, rather than holo-complex I, is sufficient to initiate mitochondrial respirasome assembly. We find that a distal part of the membrane arm of complex I (P-a module) is a scaffold for the incorporation of complexes III and IV to form a respirasome subcomplex. Read More

View Article and Full-Text PDF

Metabolic responses in neonatal sepsis - a systematic review of human metabolomic studies.

Acta Paediatr 2021 Apr 13. Epub 2021 Apr 13.

Paediatric Research Group, Faculty of Health Sciences, UiT-The Arctic University of Norway, Tromsø, Norway.

Aim: To systematically review human metabolomic studies investigating metabolic responses in septic neonates.

Methods: A systematic literature search was performed in the databases MEDLINE, EMBASE and Cochrane library up to the 1st of January 2021. We included studies that assessed neonatal sepsis and the following outcomes; (1) change in the metabolism compared to healthy neonates and/or (2) metabolomics compared to traditional diagnostic tools of neonatal sepsis. Read More

View Article and Full-Text PDF

Metformin attenuates diabetic renal injury via the AMPK-autophagy axis.

Exp Ther Med 2021 Jun 31;21(6):578. Epub 2021 Mar 31.

Department of Nephrology, General Hospital of Daqing Oil Field, Daqing, Heilongjiang 163001, P.R. China.

Diabetic nephropathy (DN) is a clinical condition characterized by kidney damage that is observed in patients with diabetes. DN is the main cause of end-stage renal disease (ESRD), which is the final stage of chronic kidney disease. Increasing evidence suggests that metformin, a characteristic oral hypoglycemic drug used for treating diabetes, exerts beneficial effects on various medical conditions and diseases, including cancer, cardiovascular diseases and thyroid-related disorders. Read More

View Article and Full-Text PDF

Effects of Shenfu Qiangxin Drink on HO-induced oxidative stress, inflammation and apoptosis in neonatal rat cardiomyocytes and possible underlying mechanisms.

Exp Ther Med 2021 Jun 26;21(6):553. Epub 2021 Mar 26.

First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China.

The aim of the present study was to investigate the effects of Shenfu Qiangxin Drink (SFQXD) on acute myocardial infarction (AMI) and identify the possible underlying mechanisms. Levels of reactive oxygen species (ROS) and inflammatory factors, including interleukin (IL)-6, IL-1β and tumor necrosis factor-α (TNF-α) in the blood samples of patients with AMI were measured using commercially available kits by visible spectrophotometry after SFQXD administration. The contents of phosphorylated (p-) forkhead box O3a (FOXO3a) was examined using an ELISA kit. Read More

View Article and Full-Text PDF

The glycolytic process in endothelial cells and its implications.

Acta Pharmacol Sin 2021 Apr 13. Epub 2021 Apr 13.

Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.

Endothelial cells play an obligatory role in regulating local vascular tone and maintaining homeostasis in vascular biology. Cell metabolism, converting food to energy in organisms, is the primary self-sustaining mechanism for cell proliferation and reproduction, structure maintenance, and fight-or-flight responses to stimuli. Four major metabolic processes take place in the energy-producing process, including glycolysis, oxidative phosphorylation, glutamine metabolism, and fatty acid oxidation. Read More

View Article and Full-Text PDF

[Angiotensin Ⅱ inhibits AMPK/SIRT1 pathway by inducing oxidative stress in RAW264.7 macrophages].

Nan Fang Yi Ke Da Xue Xue Bao 2021 Mar;41(3):384-390

General Hospital of the Yangtze River Shipping, Wuhan 430000, China.

Objective: To investigate the mechanism by which angiotensin Ⅱ-induced oxidative stress response inhibits AMPK/ SIRT1 signaling in RAW264.7 macrophages.

Objective: RAW264. Read More

View Article and Full-Text PDF

Differential DNA Methylation of the IMMP2L Gene in Families with Maternally Inherited 7q31.1 Microdeletions is Associated with Intellectual Disability and Developmental Delay.

Cytogenet Genome Res 2021 Apr 13:1-15. Epub 2021 Apr 13.

Research Institute of Medical Genetics, Tomsk National Research Medical Center, Tomsk, Russian Federation.

Most copy number variations (CNVs) in the human genome display incomplete penetrance with unknown underlying mechanisms. One such mechanism may be epigenetic modification, particularly DNA methylation. The IMMP2L gene is located in a critical region for autism susceptibility on chromosome 7q (AUTS1). Read More

View Article and Full-Text PDF

Progesterone Modulates Mitochondrial Functions in Human Glioblastoma Cells.

Mol Neurobiol 2021 Apr 13. Epub 2021 Apr 13.

Brain Research Laboratory, Department of Emergency Medicine, Emory University School of Medicine, Whitehead Biomedical Research Building, Room 655A, Atlanta, GA, 30322, USA.

A substantial literature supports the notion that cancer is a metabolic disease. Mitochondria are sexually dimorphic, and progesterone (P4) plays a key regulatory role in mitochondrial functions. We investigated the effect of P4 on mitochondrial functions in three human glioblastoma multiforme (GBM) cell lines. Read More

View Article and Full-Text PDF

Nicotinamide riboside, an NAD precursor, attenuates inflammation and oxidative stress by activating sirtuin 1 in alcohol-stimulated macrophages.

Lab Invest 2021 Apr 12. Epub 2021 Apr 12.

Department of Nutritional Sciences, University of Connecticut, Storrs, CT, USA.

Macrophages play an essential role in alcohol-induced inflammation and oxidative stress. We investigated the effects of nicotinamide riboside (NR), a natural nicotinamide adenine dinucleotide (NAD) precursor, on alcohol-induced inflammation and oxidative stress in macrophages. NR significantly decreased ethanol-induced inflammatory gene expression, with a concomitant decrease in nuclear translocation of nuclear factor κB p65 in RAW 264. Read More

View Article and Full-Text PDF

CFTR chloride channel activity modulates the mitochondrial morphology in cultured epithelial cells.

Int J Biochem Cell Biol 2021 Apr 9:105976. Epub 2021 Apr 9.

Laboratory of Cellular and Molecular Biology, Institute for Biomedical Research (BIOMED), School of Medical Sciences, Pontifical Catholic University of Argentina (UCA), the National Scientific and Technical Research Council of Argentina (CONICET), Buenos Aires, Argentina. Electronic address:

The impairment of the CFTR channel activity, a cAMP-activated chloride (Cl-) channel responsible for cystic fibrosis (CF), has been associated with a variety of mitochondrial alterations such as modified gene expression, impairment in oxidative phosphorylation, increased reactive oxygen species (ROS), and a disbalance in calcium homeostasis. The mechanisms by which these processes occur in CF are not fully understood. Previously, we demonstrated a reduced MTND4 expression and a failure in the mitochondrial complex I (mCx-I) activity in CF cells. Read More

View Article and Full-Text PDF

The long noncoding RNA lnc-HLX-2-7 is oncogenic in Group 3 medulloblastomas.

Neuro Oncol 2021 Apr;23(4):572-585

Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, School of Medicine, Johns Hopkins University, Baltimore, Maryland.

Background: Medulloblastoma (MB) is an aggressive brain tumor that predominantly affects children. Recent high-throughput sequencing studies suggest that the noncoding RNA genome, in particular long noncoding RNAs (lncRNAs), contributes to MB subgrouping. Here we report the identification of a novel lncRNA, lnc-HLX-2-7, as a potential molecular marker and therapeutic target in Group 3 MBs. Read More

View Article and Full-Text PDF

Depletion of mitochondrial inorganic polyphosphate (polyP) in mammalian cells causes metabolic shift from oxidative phosphorylation to glycolysis.

Biochem J 2021 Apr 12. Epub 2021 Apr 12.

New York University, New York City, New York, United States.

Inorganic polyphosphate (polyP) is a linear polymer composed of up to a few hundred orthophosphates linked together by high-energy phosphoanhydride bonds, identical to those found in ATP. In mammalian mitochondria, polyP has been implicated in multiple processes, including energy metabolism, ion channels function, and the regulation of calcium signaling. However, the specific mechanisms of all these effects of polyP within the organelle remain poorly understood. Read More

View Article and Full-Text PDF

Proteome analysis of human adipocytes identifies depot-specific heterogeneity at metabolic control points.

Am J Physiol Endocrinol Metab 2021 Apr 12. Epub 2021 Apr 12.

Anatomy and Physiology, University of Melbourne, Australia.

Adipose tissue is a primary regulator of energy balance and metabolism. The distribution of adipose tissue depots is of clinical interest because the accumulation of upper-body subcutaneous (ASAT) and visceral adipose tissue (VAT) is associated with cardiometabolic diseases, whereas lower-body gluteal-femoral adipose tissue (GFAT) appears to be protective. There is heterogeneity in morphology and metabolism of adipocytes obtained from different regions of the body, but detailed knowledge of the constituent proteins in each depot is lacking. Read More

View Article and Full-Text PDF

ROS-Induced Hepatotoxicity under Cypermethrin: Involvement of the Crosstalk between Nrf2/Keap1 and NF-κB/iκB-α Pathways Regulated by Proteasome.

Environ Sci Technol 2021 Apr 12. Epub 2021 Apr 12.

College of Wildlife and Protected Area, Northeast Forestry University, Harbin, Heilongjiang 150040, PR China.

Cypermethrin (CMN) is a man-made insecticide, and its abuse has led to potential adverse effects, particularly in sensitive populations such as aquatic organisms. The present study was focused on the toxic phenotype and detoxification mechanism in grass carp () after treatment with waterborne CMN (0.651 μg/L) for 6 weeks in vivo or 6. Read More

View Article and Full-Text PDF

Sickle Cell Trait Modulates the Proteome and Phosphoproteome of -Infected Erythrocytes.

Front Cell Infect Microbiol 2021 24;11:637604. Epub 2021 Mar 24.

Université de Paris, MERIT, IRD, Paris, France.

The high prevalence of sickle cell disease in some human populations likely results from the protection afforded against severe malaria and death by heterozygous carriage of HbS. remodels the erythrocyte membrane and skeleton, displaying parasite proteins at the erythrocyte surface that interact with key human proteins in the Ankyrin R and 4.1R complexes. Read More

View Article and Full-Text PDF

First comprehensive analysis of lysine succinylation in paper mulberry (Broussonetia papyrifera).

BMC Genomics 2021 Apr 10;22(1):255. Epub 2021 Apr 10.

College of Animal Science, Guizhou university, Guiyang, 550025, Guizhou, China.

Background: Lysine succinylation is a naturally occurring post-translational modification (PTM) that is ubiquitous in organisms. Lysine succinylation plays important roles in regulating protein structure and function as well as cellular metabolism. Global lysine succinylation at the proteomic level has been identified in a variety of species; however, limited information on lysine succinylation in plant species, especially paper mulberry, is available. Read More

View Article and Full-Text PDF

Mitochondrial unfolded protein response: a novel pathway in metabolism and immunity.

Pharmacol Res 2021 Apr 7:105603. Epub 2021 Apr 7.

Institute of Pharmacy and Pharmacology, Hunan Provincial Key Laboratory Of Tumor Microenvironment Responsive Drug Research, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang 421001, Hunan, China. Electronic address:

Mitochondrial unfolded protein response (mitoUPR) is a mitochondria stress response to maintain mitochondrial proteostasis during stress. Increasing evidence suggests that mitoUPR participates in diverse physiological processes especially metabolism and immunity. Although mitoUPR regulates metabolism in many aspects, it is mainly reflected in the regulation of energy metabolism. Read More

View Article and Full-Text PDF

Isorhynchophylline ameliorates paraquat-induced acute kidney injury by attenuating oxidative stress and mitochondrial damage via regulating toll-interacting expression.

Toxicol Appl Pharmacol 2021 Apr 7:115521. Epub 2021 Apr 7.

Department of Emergency Medicine, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China. Electronic address:

Isorhynchophylline (IRN) is an alkaloid with anti-inflammatory and anti-oxidative activities in cardiovascular and brain diseases, but its role in paraquat (PQ)-induced acute kidney injury (AKI) is yet unknown. The model of PQ-induced AKI in rats was established by intraperitoneal injection of PQ (25 mg/kg). We found that the tail vein injection of IRN (4 mg/kg) significantly increased the survival rate of PQ-intoxicated rats. Read More

View Article and Full-Text PDF

CCCP-induced mitochondrial dysfunction: Characterization and analysis of integrated stress response to cellular signaling and homeostasis.

FEBS J 2021 Apr 9. Epub 2021 Apr 9.

Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, 500046, Telangana, India.

Mitochondrial dysfunction mediated by CCCP (carbonyl cyanide m-chlorophenyl hydrazone), an inhibitor of mitochondrial oxidative phosphorylation, evokes the integrated stress response (ISR), which is analyzed here by eIF2α phosphorylation and expression profiles of ATF4 and CHOP proteins. Our findings suggest that the CCCP-induced ISR pathway is mediated by activation of HRI kinase, but not by GCN2, PERK, or PKR. Also, CCCP activates AMPK, a cellular energy sensor, and AKT, a regulator implicated in cell survival, and suppresses phosphorylation of mTORC1 substrates eIF4E-BP1 and S6K. Read More

View Article and Full-Text PDF

A C-terminal fragment of Arabidopsis OXIDATIVE STRESS 2 can play a positive role in salt tolerance.

Biochem Biophys Res Commun 2021 Apr 6;556:23-30. Epub 2021 Apr 6.

Plant Gene Engineering Center, Chinese Academy of Sciences Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Guangdong Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China. Electronic address:

The zinc finger transcription factor OXIDATIVE STRESS 2 (OXS2) was previously reported to be involved in oxidative stress tolerance and stress escape. Here we report that an Arabidopsis oxs2-1 mutant is also more sensitive to salt stress. Conversely, the overproduction of a C-terminal fragment of OXS2, the 'AT3' fragment, can enhance salt tolerance in Arabidopsis by upregulating the transcription of at least six salt-induced genes: COR15A, COR47, RD29B, KIN1, ACS2 and ACS6. Read More

View Article and Full-Text PDF

Multi-omics analysis of the amygdala in a rat chronic unpredictable mild stress model of depression.

Neuroscience 2021 Apr 6. Epub 2021 Apr 6.

NHC Key Laboratory of Diagnosis and Treatment on Brain Functional Diseases, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China; Department of Physical Examination, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address:

Major depressive disorder is a serious and complex mental illness, and multiple brain regions are involved in its pathogenesis. There is increasing evidence that the amygdala is involved in depression; however, the underlying molecular mechanisms remain unclear. In this study, we applied a combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based metabolomic and isobaric tags for relative and absolute quantitation (iTRAQ) proteomic to study changes in the amygdala in a chronic unpredictable mild stress (CUMS) rat model of depression. Read More

View Article and Full-Text PDF

Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.

Nucleic Acids Res 2021 Apr 9. Epub 2021 Apr 9.

Division of Medical Genetics and Genomics, The Children's Hospital, Zhejiang University School of Medicine and National Clinical Research Center for Child Health, Hangzhou, Zhejiang 310058, China.

Deficient maturations of mitochondrial transcripts are linked to clinical abnormalities but their pathophysiology remains elusive. Previous investigations showed that pathogenic variants in MTO1 for the biosynthesis of τm5U of tRNAGlu, tRNAGln, tRNALys, tRNATrp and tRNALeu(UUR) were associated with hypertrophic cardiomyopathy (HCM). Using mto1 knock-out(KO) zebrafish generated by CRISPR/Cas9 system, we demonstrated the pleiotropic effects of Mto1 deficiency on mitochondrial RNA maturations. Read More

View Article and Full-Text PDF

Periostin promotes arterial calcification through PPARγ-related glucose metabolism reprogramming.

Am J Physiol Heart Circ Physiol 2021 Apr 9. Epub 2021 Apr 9.

Department of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, P.R., China.

Extracellular matrix (ECM) exerts a list of biological functions, contributing to almost 30% of the osteogenic process. Periostin is a secreted protein that can alter ECM remodeling in response to vascular injury. However, the functional role of periostin in vascular calcification has yet to be fully described. Read More

View Article and Full-Text PDF

New 24-Membered Macrolactins Isolated from Marine Bacteria as Potent Fungal Inhibitors against Sugarcane Smut.

J Agric Food Chem 2021 Apr 9. Epub 2021 Apr 9.

Institute of Marine Drugs, School of Pharmaceutical Sciences, Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, NO. 13 Wuhe Rood, Nanning 530200, China.

Sugarcane smut, caused by , is one of the most devastating fungal diseases affecting sugarcane worldwide. To develop a potent sugarcane smut fungicide, secondary metabolites of marine-derived were isolated and screened for inhibitory activities, which led to the discovery of five new 24-membered macrolactins, bamemacrolactins A-E (-), with being the most potent inhibitor. The antifungal mechanism of was studied by assessing its effects on mycelial morphology and the cell wall. Read More

View Article and Full-Text PDF

Potential Alteration of Statin-Related Pharmacological Features in Diabetes Mellitus.

Biomed Res Int 2021 26;2021:6698743. Epub 2021 Mar 26.

Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.

Objective: Type 2 diabetes mellitus is a chronic metabolic disease caused by insulin resistance or insulin deficiency resulting in elevated blood glucose levels. Poorly controlled diabetes is associated with the development of cardiovascular disease and dyslipidemia. 3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors (statin) are an important class of therapeutic agents used to control hyperlipidemia and prevent cardiovascular disease in diabetic and nondiabetic patients. Read More

View Article and Full-Text PDF

Efficacy of Combined Electroporation-Mediated Gene Transfer of VEGF, HGF, and IL-10 on Skin Flap Survival, Monitored by Label-Free Optical Imaging: A Feasibility Study.

Front Surg 2021 23;8:639661. Epub 2021 Mar 23.

Department for BioMedical Research (DBMR), University of Bern, Bern, Switzerland.

Preventing surgical flaps necrosis remains challenging. Laser Doppler imaging and ultrasound can monitor blood flow in flap regions, but they do not directly measure the cellular response to ischemia. The study aimed to investigate the efficacy of synergistic electroporation-mediated gene transfer of interleukin 10 (IL-10) with either hepatocyte growth factor (HGF) or vascular endothelial growth factor (VEGF) on the survival of a modified McFarlane flap, and to evaluate the effect of the treatment on cell metabolism, using label-free fluorescence lifetime imaging. Read More

View Article and Full-Text PDF

Astragaloside IV Protects 6-Hydroxydopamine-Induced SH-SY5Y Cell Model of Parkinson's Disease via Activating the JAK2/STAT3 Pathway.

Front Neurosci 2021 23;15:631501. Epub 2021 Mar 23.

Department of Neurology, Hebei PetroChina Central Hospital, Langfang, China.

Objectives: Astragaloside IV (AS-IV), the main active component of Astragalus membranaceus, bears anti-inflammatory, antioxidant, and neuroprotective activity. Parkinson's disease (PD) is a common neurodegenerative disease. This study explored the protective effect of AS-IV on the cell model of PD. Read More

View Article and Full-Text PDF

Respiratory complex and tissue lineage drive recurrent mutations in tumour mtDNA.

Nat Metab 2021 Apr 8. Epub 2021 Apr 8.

Computational Oncology Service, Memorial Sloan Kettering Cancer Center, New York, NY, USA.

Mitochondrial DNA (mtDNA) encodes protein subunits and translational machinery required for oxidative phosphorylation (OXPHOS). Using repurposed whole-exome sequencing data, in the present study we demonstrate that pathogenic mtDNA mutations arise in tumours at a rate comparable to those in the most common cancer driver genes. We identify OXPHOS complexes as critical determinants shaping somatic mtDNA mutation patterns across tumour lineages. Read More

View Article and Full-Text PDF

Exploiting pyocyanin to treat mitochondrial disease due to respiratory complex III dysfunction.

Nat Commun 2021 04 8;12(1):2103. Epub 2021 Apr 8.

Department of Biology, University of Padova, Padova, Italy.

Mitochondrial diseases impair oxidative phosphorylation and ATP production, while effective treatment is still lacking. Defective complex III is associated with a highly variable clinical spectrum. We show that pyocyanin, a bacterial redox cycler, can replace the redox functions of complex III, acting as an electron shunt. Read More

View Article and Full-Text PDF