6,683 results match your criteria oxidase assembly

Glucose-Fueled Peptide Assembly: Glucagon Delivery via Enzymatic Actuation.

J Am Chem Soc 2021 Jul 19. Epub 2021 Jul 19.

University of Notre Dame, Department of Chemical & Biomolecular Engineering, Notre Dame, Indiana 46556, United States.

Nature achieves remarkable function from the formation of transient, nonequilibrium materials realized through continuous energy input. The role of enzymes in catalyzing chemical transformations to drive such processes, often as part of stimuli-directed signaling, governs both material formation and lifetime. Inspired by the intricate nonequilibrium nanostructures of the living world, this work seeks to create transient materials in the presence of a consumable glucose stimulus under enzymatic control of glucose oxidase. Read More

View Article and Full-Text PDF

Genomic analysis of a multidrug-resistant Brucella anthropi strain isolated from a 4-day-old neonatal sepsis patient.

J Glob Antimicrob Resist 2021 Jul 14;26:227-229. Epub 2021 Jul 14.

AMR Laboratory, Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Puttaparthi, India. Electronic address:

Objectives: Brucella anthropi is a Gram-negative, aerobic, motile, oxidase-positive, non-fermentative Alphaproteobacteria belonging to the family Brucellaceae. It is most commonly found in soil but is an emerging, opportunistic, nosocomial human pathogen. The objective of this study was to understand the genome features of a drug-resistant B. Read More

View Article and Full-Text PDF

sp. nov., isolated from raw milk.

Int J Syst Evol Microbiol 2021 Jul;71(7)

Chair of Microbial Ecology, ZIEL - Institute for Food & Health, Technische Universität München, Weihenstephaner Berg 3, 85354 Freising, Germany.

Two strains of a Gram-staining-positive species were isolated from German bulk tank milk. On the basis of their 16S rRNA sequences they were affiliated to the genus but could not be assigned to any species with a validly published name. ATCC BAA-466 (97. Read More

View Article and Full-Text PDF

Effects of venoms on neutrophil respiratory burst: a major inflammatory function.

J Venom Anim Toxins Incl Trop Dis 2021 28;27:e20200179. Epub 2021 Jun 28.

Université de Paris, INSERM-U1149, CNRS-ERL8252, Centre de Recherche sur l'Inflammation (CRI), Laboratoire d'Excellence Inflamex, Faculté de Médecine Xavier Bichat, Paris, France.

Neutrophils play a pivotal role in innate immunity and in the inflammatory response. Neutrophils are very motile cells that are rapidly recruited to the inflammatory site as the body first line of defense. Their bactericidal activity is due to the release into the phagocytic vacuole, called phagosome, of several toxic molecules directed against microbes. Read More

View Article and Full-Text PDF

Genes Linking Copper Trafficking and Homeostasis to the Biogenesis and Activity of the -Type Cytochrome Oxidase in the Enteric Pathogen .

Front Microbiol 2021 25;12:683260. Epub 2021 Jun 25.

Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield, United Kingdom.

Bacterial C-type haem-copper oxidases in the family are widespread in microaerophiles, which exploit their high oxygen-binding affinity for growth in microoxic niches. In microaerophilic pathogens, C-type oxidases can be essential for infection, yet little is known about their biogenesis compared to model bacteria. Here, we have identified genes involved in -oxidase (Cco) assembly and activity in the Gram-negative pathogen , the commonest cause of human food-borne bacterial gastroenteritis. Read More

View Article and Full-Text PDF

Antimycin A-induced mitochondrial dysfunction regulates inflammasome signaling in human retinal pigment epithelial cells.

Exp Eye Res 2021 Jul 1;209:108687. Epub 2021 Jul 1.

Immuno-Ophthalmology, School of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, P.O.Box 1627, FI-70211, Kuopio, Finland. Electronic address:

Age-related macular degeneration (AMD) is a severe retinal eye disease where dysfunctional mitochondria and damaged mitochondrial DNA in retinal pigment epithelium (RPE) have been demonstrated to underlie the pathogenesis of this devastating disease. In the present study, we aimed to examine whether damaged mitochondria induce inflammasome activation in human RPE cells. Therefore, ARPE-19 cells were primed with IL-1α and exposed to the mitochondrial electron transport chain complex III inhibitor, antimycin A. Read More

View Article and Full-Text PDF

Long noncoding RNA BS-DRL1 modulates the DNA damage response and genome stability by interacting with HMGB1 in neurons.

Nat Commun 2021 07 1;12(1):4075. Epub 2021 Jul 1.

Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese academy of Science, Shanghai, 200032, China.

Long noncoding RNAs (lncRNAs) are known to regulate DNA damage response (DDR) and genome stability in proliferative cells. However, it remains unknown whether lncRNAs are involved in these vital biological processes in post-mitotic neurons. Here, we report and characterize a lncRNA, termed Brain Specific DNA-damage Related lncRNA1 (BS-DRL1), in the central nervous system. Read More

View Article and Full-Text PDF

NADPH Oxidases (NOX): An Overview from Discovery, Molecular Mechanisms to Physiology and Pathology.

Antioxidants (Basel) 2021 Jun 1;10(6). Epub 2021 Jun 1.

Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale, 38000 Grenoble, France.

The reactive oxygen species (ROS)-producing enzyme NADPH oxidase (NOX) was first identified in the membrane of phagocytic cells. For many years, its only known role was in immune defense, where its ROS production leads to the destruction of pathogens by the immune cells. NOX from phagocytes catalyzes, via one-electron trans-membrane transfer to molecular oxygen, the production of the superoxide anion. Read More

View Article and Full-Text PDF

TAT-Conjugated NDUFS8 Can Be Transduced into Mitochondria in a Membrane-Potential-Independent Manner and Rescue Complex I Deficiency.

Int J Mol Sci 2021 Jun 17;22(12). Epub 2021 Jun 17.

Institute of Molecular Medicine, National Tsing Hua University, Hsinchu 30013, Taiwan.

NADH dehydrogenase (ubiquinone) Fe-S protein 8 (NDUFS8) is a nuclear-encoded core subunit of human mitochondrial complex I. Defects in NDUFS8 are associated with Leigh syndrome and encephalomyopathy. Cell-penetrating peptide derived from the HIV-1 transactivator of transcription protein (TAT) has been successfully applied as a carrier to bring fusion proteins into cells without compromising the biological function of the cargoes. Read More

View Article and Full-Text PDF

Functionalized Prion-Inspired Amyloids for Biosensor Applications.

Biomacromolecules 2021 07 1;22(7):2822-2833. Epub 2021 Jul 1.

Institut de Biotecnologia i de Biomedicina and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, 08193 Bellaterra (Barcelona), Spain.

Protein amyloid nanofibers provide a biocompatible platform for the development of functional nanomaterials. However, the functionalities generated up to date are still limited. Typical building blocks correspond to aggregation-prone proteins and peptides, which must be modified by complex and expensive reactions post-assembly. Read More

View Article and Full-Text PDF

The plant NADPH oxidase RBOHD is required for microbiota homeostasis in leaves.

Nat Microbiol 2021 Jul 30;6(7):852-864. Epub 2021 Jun 30.

Institute of Microbiology, ETH Zurich, Zurich, Switzerland.

The plant microbiota consists of a multitude of microorganisms that can affect plant health and fitness. However, it is currently unclear how the plant shapes its leaf microbiota and what role the plant immune system plays in this process. Here, we evaluated Arabidopsis thaliana mutants with defects in different parts of the immune system for an altered bacterial community assembly using a gnotobiotic system. Read More

View Article and Full-Text PDF

A DNA nanopillar as a scaffold to regulate the ratio and distance of mimic enzymes for an efficient cascade catalytic platform.

Chem Sci 2020 Oct 22;12(1):407-411. Epub 2020 Oct 22.

Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University Chongqing 400715 PR China

Herein, a rigid 3D DNA nanopillar was used to investigate the influence of spatial organization on the cascade activity in multienzyme systems, realizing controllable regulation of the mimic enzyme ratio and spacing for acquiring a high-efficiency enzyme cascade catalytic platform. Initially, the ratio of mimic enzyme AuNPs (glucose oxidase-like activity) and hemin/G-quadruplex DNAzyme (peroxidase-like activity) fixed at the designed position was adjusted by changing the number of edges in a DNA polyhedron, resulting in an optimal mimic enzyme ratio of 1 : 4 with a quadrangular prism as the scaffold. Notably, the DNA nanopillar formed by quadrangular prism layer-by-layer assembly acted as a track for directional and controllable movement of a bipedal DNA walker based on the toehold mediated strand displacement reaction (TSDR), which endowed the assay system with continuous enzyme spacing regulation compared with previous enzyme cascade systems that induced inflexible operation. Read More

View Article and Full-Text PDF
October 2020

Self-programmed enzyme phase separation and multiphase coacervate droplet organization.

Chem Sci 2021 Jan 25;12(8):2794-2802. Epub 2021 Jan 25.

Univ. Bordeaux, CNRS, Centre de Recherche Paul Pascal, UMR5031 115 Avenue du Dr Schweitzer 33600 Pessac France

Membraneless organelles are phase-separated droplets that are dynamically assembled and dissolved in response to biochemical reactions in cells. Complex coacervate droplets produced by associative liquid-liquid phase separation offer a promising approach to mimic such dynamic compartmentalization. Here, we present a model for membraneless organelles based on enzyme/polyelectrolyte complex coacervates able to induce their own condensation and dissolution. Read More

View Article and Full-Text PDF
January 2021

Evolution of an assembly factor-based subunit contributed to a novel NDH-PSI supercomplex formation in chloroplasts.

Nat Commun 2021 06 17;12(1):3685. Epub 2021 Jun 17.

Department of Botany, Graduate School of Science, Kyoto University, Kyoto, Japan.

Chloroplast NADH dehydrogenase-like (NDH) complex is structurally related to mitochondrial Complex I and forms a supercomplex with two copies of Photosystem I (the NDH-PSI supercomplex) via linker proteins Lhca5 and Lhca6. The latter was acquired relatively recently in a common ancestor of angiosperms. Here we show that NDH-dependent Cyclic Electron Flow 5 (NDF5) is an NDH assembly factor in Arabidopsis. Read More

View Article and Full-Text PDF

Self-Assembly Protein Superstructures as a Powerful Chemodynamic Therapy Nanoagent for Glioblastoma Treatment.

Nanomicro Lett 2020 Jul 15;12(1):151. Epub 2020 Jul 15.

Department of Health Technology, Technical University of Denmark, 2800, Kongens Lyngby, Denmark.

Glioblastoma (GBM) remains a formidable challenge in oncology. Chemodynamic therapy (CDT) that triggers tumor cell death by reactive oxygen species (ROS) could open up a new door for GBM treatment. Herein, we report a novel CDT nanoagent. Read More

View Article and Full-Text PDF

Real-Time NMR Monitoring of Spatially Segregated Enzymatic Reactions in Multilayered Hydrogel Assemblies*.

Angew Chem Int Ed Engl 2021 Jun 16. Epub 2021 Jun 16.

Institute of Microstructure Technology, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.

Compartmentalized chemical reactions at the microscale are important in biotechnology, yet monitoring the molecular content at these small scales is challenging. To address this challenge, we integrate a compact, reconfigurable reaction cell featuring electrochemical functionality with high-resolution NMR spectroscopy. We demonstrate the operation of this system by monitoring the activity of enzymes immobilized in chemically distinct layers within a multi-layered chitosan hydrogel assembly. Read More

View Article and Full-Text PDF

Construction of coacervate-in-coacervate multi-compartment protocells for spatial organization of enzymatic reactions.

Chem Sci 2020 Aug 7;11(32):8617-8625. Epub 2020 Aug 7.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University Changsha 410082 P. R. China

Coacervate microdroplets, formed liquid-liquid phase separation, have been extensively explored as a compartment model for the construction of artificial cells or organelles. In this study, coacervate-in-coacervate multi-compartment protocells were constructed using four polyelectrolytes, in which carboxymethyl-dextran and diethylaminoethyl-dextran were deposited on the surface of as-prepared polydiallyldimethyl ammonium/deoxyribonucleic acid coacervate microdroplets through layer-by-layer assembly. The resulting multi-compartment protocells were composed from two immiscible coacervate phases with distinct physical and chemical properties. Read More

View Article and Full-Text PDF

Schizosaccharomyces pombe Ppr10 and Mpa1 together mediate mitochondrial translational initiation.

J Biol Chem 2021 Jun 10;297(1):100869. Epub 2021 Jun 10.

Jiangsu Key Laboratory for Microbes and Genomics, School of Life Sciences, Nanjing Normal University, Nanjing, China. Electronic address:

Pentatricopeptide repeat (PPR) proteins are a large family of proteins that act primarily at different posttranscriptional steps of organellar gene expression. We have previously found that the Schizosaccharomyces pombe PPR protein mpal10 interacts with mitochondrial translational activator Mpa1, and both are essential for mitochondrial protein synthesis. However, it is unclear how these two proteins function in mitochondrial protein synthesis in S. Read More

View Article and Full-Text PDF

Severe multisystem pathology, metabolic acidosis, mitochondrial dysfunction, and early death associated with an X-linked variant.

Cold Spring Harb Mol Case Stud 2021 Jun 11;7(3). Epub 2021 Jun 11.

Greenwood Genetic Center, Greenwood, South Carolina 29646, USA.

Variants in the X-linked gene (apoptosis-inducing factor mitochondria-associated 1) are associated with a highly variable clinical presentation that encompasses motor neuropathy, ataxia, encephalopathies, deafness, and cognitive impairment. encodes a mitochondrial flavin adenine dinucleotide (FAD)-dependent nicotinamide adenine dinucleotide (NADH) oxidoreductase, with roles in the regulation of respiratory complex assembly and function, production of reactive oxygen species, and the coordination of a caspase-independent type of apoptosis known as parthanatos. In this report, we describe a missense variant (absent in reference population databases; c. Read More

View Article and Full-Text PDF

A chromosome-level Camptotheca acuminata genome assembly provides insights into the evolutionary origin of camptothecin biosynthesis.

Nat Commun 2021 06 10;12(1):3531. Epub 2021 Jun 10.

Key Laboratory of Bio-resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.

Camptothecin and its derivatives are widely used for treating malignant tumors. Previous studies revealed only a limited number of candidate genes for camptothecin biosynthesis in Camptotheca acuminata, and it is still poorly understood how its biosynthesis of camptothecin has evolved. Here, we report a high-quality, chromosome-level C. Read More

View Article and Full-Text PDF

Overexpression of D-dopachrome tautomerase increases ultraviolet B irradiation-induced skin tumorigenesis in mice.

FASEB J 2021 07;35(7):e21671

Department of Dermatology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan.

Ultraviolet irradiation (UV) exposure is the leading factor underlying the development of skin malignancies. D-dopachrome tautomerase (D-DT), a functional homolog of macrophage migration inhibitory factor (MIF), has functional similarities to MIF. However, its role, unlike the role of MIF in photocarcinogenesis, is unknown. Read More

View Article and Full-Text PDF

Probing Selective Self-Assembly of Putrescine Oxidase with Controlled Orientation Using a Genetically Engineered Peptide Tag.

Langmuir 2021 06 8;37(24):7536-7547. Epub 2021 Jun 8.

Department of Chemistry, University of Kansas, Lawrence, Kansas 66045, United States.

Controlling enzyme orientation and location on surfaces is a critical step for their successful deployment in diverse applications from biosensors to lab-on-a-chip devices. Functional activity of the enzymes on the surface will largely depend on the spatial arrangement and orientation. Solid binding peptides have been proven to offer versatility for immobilization of biomolecules on inorganic materials including metals, oxides, and minerals. Read More

View Article and Full-Text PDF

The atypical small GTPase GEM/Kir is a negative regulator of the NADPH oxidase and NETs production through macroautophagy.

J Leukoc Biol 2021 Jun 4. Epub 2021 Jun 4.

Department of Molecular Medicine, The Scripps Research Institute, La Jolla, California, USA.

Despite the important function of neutrophils in the eradication of infections and induction of inflammation, the molecular mechanisms regulating the activation and termination of the neutrophil immune response is not well understood. Here, the function of the small GTPase from the RGK family, Gem, is characterized as a negative regulator of the NADPH oxidase through autophagy regulation. Gem knockout (Gem KO) neutrophils show increased NADPH oxidase activation and increased production of extracellular and intracellular reactive oxygen species (ROS). Read More

View Article and Full-Text PDF

Association of COA1 with Patellar Tendonitis: A Genome-wide Association Analysis.

Med Sci Sports Exerc 2021 May 28. Epub 2021 May 28.

Dept. Dev. Biology, Stanford University School of Medicine, Stanford, CA Kaiser Permanente Northern California, Division of Research, Oakland, CA Dept. Orthopaedic Surgery, Stanford University School of Medicine, Stanford, CA.

Purpose: It is unknown why some athletes develop patellar tendinopathy and others do not, even when accounting for similar workloads between individuals. Genetic differences between these two populations may be a contributing factor. The purpose of this work was to screen the entire genome for genetic markers associated with patellar tendinopathy. Read More

View Article and Full-Text PDF

Analysis of the Coptis chinensis genome reveals the diversification of protoberberine-type alkaloids.

Nat Commun 2021 06 2;12(1):3276. Epub 2021 Jun 2.

Hubei Institute for Drug Control, Wuhan, China.

Chinese goldthread (Coptis chinensis Franch.), a member of the Ranunculales, represents an important early-diverging eudicot lineage with diverse medicinal applications. Here, we present a high-quality chromosome-scale genome assembly and annotation of C. Read More

View Article and Full-Text PDF

Mitochondrial Reactive Oxygen Species Enhance Alveolar Macrophage Activity against Aspergillus fumigatus but Are Dispensable for Host Protection.

mSphere 2021 Jun 2;6(3):e0026021. Epub 2021 Jun 2.

Infectious Disease Service, Department of Medicine, Memorial Hospital, Memorial Sloan Kettering Cancer Center, New York, New York, USA.

Aspergillus fumigatus is the most common cause of mold pneumonia worldwide, and a significant cause of infectious morbidity and mortality in immunocompromised individuals. The oxidative burst, which generates reactive oxidative species (ROS), plays a pivotal role in host defense against aspergillosis and induces regulated cell death in Aspergillus conidia, the infectious propagules. Beyond the well-established role of NADP (NADPH) oxidase in ROS generation by neutrophils and other innate effector cells, mitochondria represent a major ROS production site in many cell types, though it is unclear whether mitochondrial ROS (mtROS) contribute to antifungal activity in the lung. Read More

View Article and Full-Text PDF

Restoring the Oxidase-Like Activity of [email protected] for the Determination of Alkaline Phosphatase.

Biosensors (Basel) 2021 May 30;11(6). Epub 2021 May 30.

College of Life Sciences, Northwest Normal University, Lanzhou 730070, China.

In this paper, we propose a simple colorimetric method for the sensitive and selective detection of alkaline phosphatase (ALP) activity based on the turn off/turn on oxidase mimic activity of [email protected] [email protected]/graphene oxide hybrids ([email protected]/GO) were easily obtained using the self-assembly method with poly (diallyldimethylammonium chloride) (PDDA)-coated GO and showed high oxidase-like activity compared with [email protected] We found that the pyrophosphate ion (PO, PPi) could effectively inhibit the oxidase mimic activity of [email protected]/GO, and the hydrolysis of PPi by ALP restored the inhibited activity of [email protected]/GO, enabling them to efficiently catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to generate the blue oxidized product oxTMB. Read More

View Article and Full-Text PDF

Diabetic Retinopathy and NADPH Oxidase-2: A Sweet Slippery Road.

Renu A Kowluru

Antioxidants (Basel) 2021 May 15;10(5). Epub 2021 May 15.

Department of Ophthalmology, Visual and Anatomical Sciences, Kresge Eye Institute, Wayne State University, Detroit, MI 48201, USA.

Diabetic retinopathy remains the leading cause of vision loss in working-age adults. The multi-factorial nature of the disease, along with the complex structure of the retina, have hindered in elucidating the exact molecular mechanism(s) of this blinding disease. Oxidative stress appears to play a significant role in its development and experimental models have shown that an increase in cytosolic Reacttive Oxygen Speies (ROS) due to the activation of NADPH oxidase 2 (Nox2), is an early event, which damages the mitochondria, accelerating loss of capillary cells. Read More

View Article and Full-Text PDF

One-step self-assembly of magnetic supramolecular metal-organic coordination functionalized MoS complex as nanoenzyme-reactor.

Colloids Surf B Biointerfaces 2021 Sep 26;205:111879. Epub 2021 May 26.

State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China. Electronic address:

In the present study, a kind of magnetic supramolecular metal-organic coordination complex (SMOCC) functionalized MoS was prepared with one-step in aqueous solution for enzyme immobilization. As possessing a protective nanocoating of PDA/PEI/Cu (polydopamine: PDA, polyethyleneimine: PEI), the proposed material can provide biocompatible microenvironment and flexible adhesion force on particle interface, which is conductive to loading laccase (170.0 ± 1. Read More

View Article and Full-Text PDF
September 2021

Sheltering proteins from protease-mediated degradation and a strategy for preventing acute liver injury.

Biomater Sci 2021 Jun;9(12):4423-4427

Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province 325000, P. R. China.

Restoring protein functions or supplying proteins is considered one of the most powerful therapeutic strategies for many diseases, but it is mainly limited by the denaturation of proteins during encapsulation and degradation by proteases during in vivo delivery, and limits its delivery. Herein, by encapsulating a protein (catalase, an enzyme) in a hexahistidine-metal assembly (HmA) via a de novo strategy under mild conditions, we demonstrated that HmA could maintain the bioactivity of the enzyme, protect the enzyme from proteinase degradation, and deliver the encapsulated protein for the prevention of disease in an acute liver injury model. Read More

View Article and Full-Text PDF