17 results match your criteria overlap nanosized

  • Page 1 of 1

Super-resolution Microscopy-based Bimolecular Fluorescence Complementation to Study Protein Complex Assembly and Co-localization.

Bio Protoc 2020 Feb 20;10(4):e3524. Epub 2020 Feb 20.

Stowers Institute for Medical Research, Kansas City, MO 64110, USA.

Numerous experimental approaches exist to study interactions between two subunits of a large macromolecular complex. However, most methods do not provide spatial and temporal information about binding, which are critical for dissecting the mechanism of assembly of nanosized complexes . While recent advances in super-resolution microscopy techniques have provided insights into biological structures beyond the diffraction limit, most require extensive expertise and/or special sample preparation, and it is a challenge to extend beyond binary, two color experiments. Read More

View Article and Full-Text PDF
February 2020

Abundantly Present miRNAs in Milk-Derived Extracellular Vesicles Are Conserved Between Mammals.

Front Nutr 2018 18;5:81. Epub 2018 Sep 18.

Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine Utrecht University, Utrecht, Netherlands.

Mammalian milk is not only a source of nutrition for the newborn, but also contains various components that regulate further development. For instance, milk is an abundant source of microRNAs (miRNAs), which are evolutionary conserved small non-coding RNAs that are involved in post-transcriptional regulation of target mRNA. MiRNAs present in milk can occur in extracellular vesicles (EVs), which are nanosized membrane vesicles released by many cell types as a means of intercellular communication. Read More

View Article and Full-Text PDF
September 2018

DNA Interaction with Head-to-Tail Associates of Cationic Surfactants Prevents Formation of Compact Particles.

Molecules 2018 Jun 28;23(7). Epub 2018 Jun 28.

Experimental Physics, Institute of Physics and Astronomy, University of Potsdam, 14476 Potsdam-Golm, Germany.

Cationic azobenzene-containing surfactants are capable of condensing DNA in solution with formation of nanosized particles that can be employed in gene delivery. The ratio of surfactant/DNA concentration and solution ionic strength determines the result of DNA-surfactant interaction: Complexes with a micelle-like surfactant associates on DNA, which induces DNA shrinkage, DNA precipitation or DNA condensation with the emergence of nanosized particles. UV and fluorescence spectroscopy, low gradient viscometry and flow birefringence methods were employed to investigate DNA-surfactant and surfactant-surfactant interaction at different NaCl concentrations, [NaCl]. Read More

View Article and Full-Text PDF

Reversible thermodynamic cycle analysis for capacitive deionization with modified Donnan model.

J Colloid Interface Sci 2018 Feb 17;512:522-528. Epub 2017 Oct 17.

Department of Civil and Environmental Engineering, Vanderbilt University, Nashville, TN 37235-1831, USA; Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN 37235-1604, USA. Electronic address:

It is a widely accepted principle that a thermodynamically reversible desalination process should consume the Gibbs free energy of separation. This principle has been shown in reverse osmosis and has important practical implications in reducing its energy consumption. Capacitive deionization (CDI) with carbon electrodes, a desalination process based on electrical double layer (EDL) formation, should also follow such a principle when it operates in a thermodynamically reversible way. Read More

View Article and Full-Text PDF
February 2018

Application of X-ray Diffraction and Electron Crystallography for Solving Complex Structure Problems.

Acc Chem Res 2017 11 1;50(11):2737-2745. Epub 2017 Nov 1.

College of Chemistry and Molecular Engineering, Peking University , Yiheyuan Road 5, Beijing 100871, China.

All crystalline materials in nature, whether inorganic, organic, or biological, macroscopic or microscopic, have their own chemical and physical properties, which strongly depend on their atomic structures. Therefore, structure determination is extremely important in chemistry, physics, materials science, etc. In the past centuries, many techniques have been developed for structure determination. Read More

View Article and Full-Text PDF
November 2017

Electrostatic interaction between nonuniformly charged colloids: experimental and numerical study.

Langmuir 2015 Feb 27;31(5):1649-59. Epub 2015 Jan 27.

Laboratoire Charles Coulomb (L2C), UMR 5221 CNRS-Université de Montpellier , Montpellier, France.

The influence of the surface charge distribution on the interaction between nanosized particles in water is reported. The distribution of charges at the surface of initially neutral microemulsion droplets has been modulated by additions of various oligomeric cationic surfactants. The osmotic compressibility of the doped microemulsions was measured by light and small-angle neutrons scattering and reveals that the overall effective interaction induced by the ionic groups is repulsive. Read More

View Article and Full-Text PDF
February 2015

Density functional theory modeling of multilayer "epitaxial" graphene oxide.

Acc Chem Res 2014 Nov 20;47(11):3331-9. Epub 2014 May 20.

School of Physics, School of Chemistry and Biochemistry, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.

CONSPECTUS: Graphene oxide (GO) is a complex material of both fundamental and applied interest. Elucidating the structure of GO is crucial to achieve control over its properties and technological applications. GO is a nonstoichiometric and hygroscopic material with a lamellar structure, and its physical chemical properties depend critically on synthesis procedures and postsynthesis treatments. Read More

View Article and Full-Text PDF
November 2014

Genetic dissection of the mamAB and mms6 operons reveals a gene set essential for magnetosome biogenesis in Magnetospirillum gryphiswaldense.

J Bacteriol 2014 Jul 9;196(14):2658-69. Epub 2014 May 9.

Ludwig-Maximilians-Universität München, Departement Biologie I, Mikrobiologie, LMU Biozentrum, Planegg Martinsried, Germany

Biosynthesis of bacterial magnetosomes, which are intracellular membrane-enclosed, nanosized magnetic crystals, is controlled by a set of >30 specific genes. In Magnetospirillum gryphiswaldense, these are clustered mostly within a large conserved genomic magnetosome island (MAI) comprising the mms6, mamGFDC, mamAB, and mamXY operons. Here, we demonstrate that the five previously uncharacterized genes of the mms6 operon have crucial functions in the regulation of magnetosome biomineralization that partially overlap MamF and other proteins encoded by the adjacent mamGFDC operon. Read More

View Article and Full-Text PDF

Phonon lateral confinement enables thermal rectification in asymmetric single-material nanostructures.

Nano Lett 2014 Feb 9;14(2):592-6. Epub 2014 Jan 9.

School of Mechanical Engineering, Purdue University , West Lafayette, Indiana 47907, United States.

We show that thermal rectification (TR) in asymmetric graphene nanoribbons (GNRs) is originated from phonon confinement in the lateral dimension, which is a fundamentally new mechanism different from that in macroscopic heterojunctions. Our molecular dynamics simulations reveal that, though TR is significant in nanosized asymmetric GNRs, it diminishes at larger width. By solving the heat diffusion equation, we prove that TR is indeed absent in both the total heat transfer rate and local heat flux for bulk-size asymmetric single materials, regardless of the device geometry or the anisotropy of the thermal conductivity. Read More

View Article and Full-Text PDF
February 2014

Effect of linear nonionic polymer additives on the kinetic stability of dispersions of poly(diallyldimethylammonium chloride)/sodium dodecylsulfate nanoparticles.

Langmuir 2013 Aug 1;29(32):10077-86. Epub 2013 Aug 1.

Laboratory of Interfaces and Nanosized Systems, Institute of Chemistry, Eötvös Loránd University, 1117 Budapest, Pázmány Péter Sétány 1/A, Hungary.

In this article, the impact of different neutral polymers on the kinetic stability of charge-stabilized poly(diallyldimethylammonium chloride) (PDADMAC)/sodium dodecylsulfate (SDS) colloidal dispersions is analyzed using dynamic light scattering, electrophoretic mobility, turbidity, and coagulation kinetics measurements. Poly(ethyleneoxide) (PEO), poly(vinylpyrrolidone) (PVP), and dextran of comparable molecular masses as well as a higher-molecular-weight dextran sample were tested as nonionic additives. The light scattering and mobility data indicate that the PEO and PVP molecules may adsorb on the surface of the PDADMAC/SDS nanoparticles formed in the presence of excess surfactant. Read More

View Article and Full-Text PDF

Growth of Hexagonal Columnar Nanograin Structured SiC Thin Films on Silicon Substrates with Graphene-Graphitic Carbon Nanoflakes Templates from Solid Carbon Sources.

Materials (Basel) 2013 Apr 16;6(4):1543-1553. Epub 2013 Apr 16.

Key Laboratory of Semiconductor Materials Science, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China.

We report a new method for growing hexagonal columnar nanograin structured silicon carbide (SiC) thin films on silicon substrates by using graphene-graphitic carbon nanoflakes (GGNs) templates from solid carbon sources. The growth was carried out in a conventional low pressure chemical vapor deposition system (LPCVD). The GGNs are small plates with lateral sizes of around 100 nm and overlap each other, and are made up of nanosized multilayer graphene and graphitic carbon matrix (GCM). Read More

View Article and Full-Text PDF

Generation of intracellular reactive oxygen species and genotoxicity effect to exposure of nanosized polyamidoamine (PAMAM) dendrimers in PLHC-1 cells in vitro.

Aquat Toxicol 2013 May 8;132-133:61-72. Epub 2013 Feb 8.

Radiation and Environmental Science Centre, Focas Research Institute, Dublin Institute of Technology, Kevin Street, Dublin 8, Ireland; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Polyamidoamine (PAMAM) dendrimers have previously been demonstrated to elicit systematically variable cyto- and eco-toxic responses, promising as the basis for structure-activity relationships governing nanotoxicological responses. In this study, increased production of intracellular reactive oxygen species (ROS), genotoxicity and apoptosis due to in vitro exposure of fish hepatocellular carcinoma cells to dendrimer generations G4, G5 and G6 is demonstrated. A PAMAM dendrimer generation dependent increase in ROS and genotoxicity was observed, consistent with our previous studies. Read More

View Article and Full-Text PDF

Enhancement of field-analyte interaction at metallic nanogap arrays for sensitive localized surface plasmon resonance detection.

Appl Opt 2012 Nov;51(31):7437-42

Department of Biomedical Engineering, Kyung Hee University, Yongin, South Korea.

We investigated the near-field enhancement of a localized surface plasmon resonance (LSPR) structure based on gold nanograting pairs with a nanosized gap. The results calculated by finite-difference time-domain and rigorous coupled-wave analysis methods presented that the nanogap enclosed by two neighboring nanogratings produced significant confinement and enhancement of electromagnetic fields and allowed a sensitive detection in sensing of surface binding events. Gold gratings with a narrow gap distance less than 10 nm showed enhanced refractive index sensitivity due to the intensified optical field at the nanogap, outperforming the LSPR structure with noninteracting nanogratings. Read More

View Article and Full-Text PDF
November 2012

Förster resonance energy transfer among a structural isomer of adenine and various Coumarins inside a nanosized reverse micelle.

Spectrochim Acta A Mol Biomol Spectrosc 2012 Apr 27;89:67-73. Epub 2011 Dec 27.

Department of Chemistry, Indian Institute of Technology, Kharagpur 721302, WB, India.

In this article we have studied Förster Resonance Energy Transfer (FRET) using 2-aminopurine (2-AP), a structural isomer of adenine as donor and various Coumarins as acceptors inside AROSOL-OT (AOT)-water reverse micelles (RM) using steady-state and time-resolved fluorescence spectroscopies. We have used three sets of FRET and all the pairs except 2-AP-Coumarin-480 exhibited quite efficient FRET. For the efficient pairs, overlap integral J(λ) and Förster distance (R0) are of high values but the rate constant of energy transfer (kET) are quite low. Read More

View Article and Full-Text PDF

Photon energy dependent valence band response of metallic nanoparticles.

Phys Rev Lett 2011 Oct 25;107(18):183401. Epub 2011 Oct 25.

Department of Physics, University of Oulu, PO Box 3000, 90014 Oulu, Finland.

We show that the valence band response to photon impact in metallic nanoparticles is highly energy dependent. This is seen as drastic variations of cross sections in valence photoionization of free and initially charge-neutral nanosized metal clusters. The effect is demonstrated in a combined experimental and theoretical study of Rb clusters. Read More

View Article and Full-Text PDF
October 2011

Theoretical studies on iron surface coating: adsorption of furan derivatives over Fe(n) clusters (n = 1-4).

J Nanosci Nanotechnol 2011 Jun;11(6):5483-90

Investigaciones Químicas y Ciencias de la Tierra, Universidad Autónoma del Estado de Hidalgo, Unidad Universitaria, Km 4.5 Carretera Pachuca-Tulancingo, C.P. 42184 Pachuca-Hidalgo, México.

In the transition from small molecules to solids, atomic clusters are studied widely not only because they can own unique properties due to the nanosized effect but also because they represent an intermediate stage. A systematic study on the structures and properties of clusters as a function of size could give information on the transition from clusters to bulk and determine at what size a cluster can mimic the bulk solid at least to some extent. The adsorption capability of furan derivatives 2-furfurylamine, 2-furfuryl alcohol and 2-furfuryl mercaptan with different iron-clusters was studied by DFT. Read More

View Article and Full-Text PDF

Water-swellable polyelectrolyte microgels polymerized in an inverse microemulsion using a nonionic surfactant.

J Colloid Interface Sci 2004 Jul;275(2):450-7

Technology Development Research Labs., Material Science Research Center, Shiseido Co., Ltd., 2-2-1 Hayabuchi, Tsuzuki, Yokohama 224-8558, Japan.

A series of poly(dimethylacrylamide-co-2-acrylamido-2-methyl-1-propanesulfonic acid) microgels slightly crosslinked by methylene-bis-acrylamide (MBA) were polymerized in a novel inverse microemulsion polymerization (IMEP) system. To determine a suitable composition of the IMEP system, the phase diagram of a pseudoternary system was made. The pseudoternary polymerization system consisted of n-hexane, a nonionic surfactant (polyoxyethylene oleyl ether, C18En), and an aqueous monomer solution. Read More

View Article and Full-Text PDF
  • Page 1 of 1