5,749 results match your criteria overexpression strains


The DnaK/DnaJ Chaperone System Enables RNA Polymerase-DksA Complex Formation in Salmonella Experiencing Oxidative Stress.

mBio 2021 May 11;12(3). Epub 2021 May 11.

University of Colorado School of Medicine, Department of Immunology & Microbiology, Aurora, Colorado, USA

Our previous biochemical approaches showed that the oxidoreductase activity of the DnaJ protein facilitates the interaction of oxidized DksA with RNA polymerase. Investigations herein demonstrate that under biologically relevant conditions the DnaJ- and DksA-codependent activation of the stringent response in undergoing oxidative stress involves the DnaK chaperone. Oxidation of DksA cysteine residues stimulates redox-based and holdase interactions with zinc-binding and C-terminal domains of DnaJ. Read More

View Article and Full-Text PDF

ATP-dependent protease ClpP and its subunits ClpA, ClpB, and ClpX involved in the field bismerthiazol-resistance in pv. .

Phytopathology 2021 May 11. Epub 2021 May 11.

Plant Protection, Nanjing, Jiangsu, China;

Resistance of pv. (), which causes rice bacterial leaf blight, to bismerthiazol has been detected in China since the 1990s. Interestingly, the strains resistant to bismerthiazol on rice plants were more sensitive to bismerthiazol than wild-type (WT) strains . Read More

View Article and Full-Text PDF

Staphylococcus aureus ST228 and ST239 as models for expression studies of diverse markers during osteoblast infection and persistence.

Microbiologyopen 2021 Mar;10(2):e1178

Department of Biomedical and Biotechnological Sciences (BIOMETEC), Medical Molecular Microbiology and Antibiotic Resistance laboratory (MMARLab, University of Catania, Catania, Italy.

The ability of S. aureus to infect bone and osteoblasts is correlated with its incredible virulence armamentarium that can mediate the invasion/internalization process, cytotoxicity, membrane damage, and intracellular persistence. We comparatively analyzed the interaction, persistence, and modulation of expression of selected genes and cell viability in an ex vivo model using human MG-63 osteoblasts of two previously studied and well-characterized S. Read More

View Article and Full-Text PDF

C-metabolic flux analysis in glycerol-assimilating strains of Saccharomyces cerevisiae.

J Gen Appl Microbiol 2021 May 8. Epub 2021 May 8.

School of Life Science and Technology, Tokyo Institute of Technology.

Glycerol is an attractive raw material for the production of useful chemicals using microbial cells. We previously identified metabolic engineering targets for the improvement of glycerol assimilation ability in Saccharomyces cerevisiae based on adaptive laboratory evolution (ALE) and transcriptome analysis of the evolved cells. We also successfully improved glycerol assimilation ability by the disruption of the RIM15 gene encoding a Greatwall protein kinase together with overexpression of the STL1 gene encoding the glycerol/H symporter. Read More

View Article and Full-Text PDF

Fol-milR1, a pathogenicity factor of Fusarium oxysporum, confers tomato wilt disease resistance by impairing host immune responses.

New Phytol 2021 May 7. Epub 2021 May 7.

College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, JS, 225009, China.

Although miRNAs are well known to play crucial roles in multiple biological processes, there is currently no evidence indicating milRNAs from Fusarium oxysporum f.sp. lycopersici (Fol) interfere with tomato resistance during infection. Read More

View Article and Full-Text PDF

Genome-wide Screening of Genes Associated with Momilactone B Sensitivity in the Fission Yeast.

G3 (Bethesda) 2021 May 6. Epub 2021 May 6.

Agro-Biotechnology Research Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.

Momilactone B is a natural product with dual biological activities, including antimicrobial and allelopathic properties, and play a major role in plant chemical defense against competitive plants and pathogens. The pharmacological effects of momilactone B on mammalian cells have also been reported. However, little is known about the molecular and cellular mechanisms underlying its broad bioactivity. Read More

View Article and Full-Text PDF

Bmcas-1 plays an important role in response against BmNPV infection in vitro.

Arch Insect Biochem Physiol 2021 May 5:e21793. Epub 2021 May 5.

Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China.

Apoptosis, as one kind of innate immune system, is involved in host response against pathogens innovation. Caspases play a vital role in the execution stage of host cell apoptosis. It has been reported that Bmcaspase-1 (Bmcas-1) has a close relationship with Bombyx mori nucleopolyhedrovirus (BmNPV) infection for its differentially expressed patterns after viral infection. Read More

View Article and Full-Text PDF

High-efficiency transformation of Streptococcus thermophilus using electroporation.

J Sci Food Agric 2021 May 3. Epub 2021 May 3.

Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.

Background: Streptococcus thermophilus, one of the most important lactic acid bacteria, is widely used in food fermentation, which is beneficial to improve food quality. However, the current genetic transformation systems are inefficient for S. thermophilus S-3, which hinders its further study. Read More

View Article and Full-Text PDF

Comparative genome analysis of multidrug-resistant Pseudomonas aeruginosa JNQH-PA57, a clinically isolated mucoid strain with comprehensive carbapenem resistance mechanisms.

BMC Microbiol 2021 May 1;21(1):133. Epub 2021 May 1.

Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, Shandong, China.

Background: The prevalence of clinical multidrug-resistant (MDR) Pseudomonas aeruginosa has been increasing rapidly worldwide over the years and responsible for a wide range of acute and chronic infections with high mortalities. Although hundreds of complete genomes of clinical P. aeruginosa isolates have been sequenced, only a few complete genomes of mucoid strains are available, limiting a comprehensive understanding of this important group of opportunistic pathogens. Read More

View Article and Full-Text PDF

Specialized Metabolites from Ribosome Engineered Strains of .

Metabolites 2021 Apr 13;11(4). Epub 2021 Apr 13.

Department of Biology, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada.

Bacterial specialized metabolites are of immense importance because of their medicinal, industrial, and agricultural applications. is a known producer of such compounds; however, much of its metabolic potential remains unknown, as many associated biosynthetic gene clusters are silent or expressed at low levels. The overexpression of ribosome recycling factor () and ribosome engineering (induced mutations) in other spp. Read More

View Article and Full-Text PDF

Characterization of Two Unique Cold-Active Lipases Derived from a Novel Deep-Sea Cold Seep Bacterium.

Microorganisms 2021 Apr 10;9(4). Epub 2021 Apr 10.

College of Life Sciences, Qingdao University, Qingdao 266071, China.

The deep ocean microbiota has unexplored potential to provide enzymes with unique characteristics. In order to obtain cold-active lipases, bacterial strains isolated from the sediment of the deep-sea cold seep were screened, and a novel strain gcc21 exhibited a high lipase catalytic activity, even at the low temperature of 4 °C. The strain gcc21 was identified and proposed to represent a new species of according to its physiological, biochemical, and genomic characteristics; it was named . Read More

View Article and Full-Text PDF

Genetically Engineered Oleaginous Yeast for Sesquiterpene α-Zingiberene Production.

ACS Synth Biol 2021 Apr 29. Epub 2021 Apr 29.

Chemical and Biological Processes Development Group, Pacific Northwest National Laboratory, Richland, Washington 99352, United States.

Oleaginous yeast, such as , are logical organisms for production of higher energy density molecules like lipids and terpenes. We demonstrate that transgenic strains expressing an α-zingiberene synthase gene from lemon basil or Hall's panicgrass can produce up to 17 mg/L α-zingiberene in yeast extract peptone dextrose (YPD) medium containing 4% glucose. The transgenic strain was further examined in 8% glucose media with C/N ratios of 20 or 100, and YPD. Read More

View Article and Full-Text PDF

Role of the mitochondrial citrate-oxoglutarate carrier in lipid accumulation in the oleaginous fungus Mortierella alpina.

Biotechnol Lett 2021 Apr 27. Epub 2021 Apr 27.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China.

Objectives: The transport of citrate from the mitochondria to the cytoplasm is essential during lipid accumulation. This study aimed to explore the role of mitochondrial citrate-oxoglutarate carrier in lipid accumulation in the oleaginous fungus Mortierella alpina.

Results: Homologous MaYHM (the gene encoding the mitochondrial citrate-oxoglutarate carrier) was overexpressed in M. Read More

View Article and Full-Text PDF

The anti-sigma factor MucA is required for viability in Pseudomonas aeruginosa.

Mol Microbiol 2021 Apr 27. Epub 2021 Apr 27.

School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV, USA.

During decades-long infections in the cystic fibrosis (CF) airway, Pseudomonas aeruginosa undergoes selection. One bacterial genetic adaptation often observed in CF isolates is mucA mutations. MucA inhibits the sigma factor AlgU. Read More

View Article and Full-Text PDF

Improvement of Free Fatty Acid Secretory Productivity in by Comprehensive Analysis on Time-Series Gene Expression.

Front Microbiol 2021 9;12:605095. Epub 2021 Apr 9.

Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan.

is a filamentous fungus that has historically been utilized in the fermentation of food products. In recent times, it has also been introduced as a component in the industrial biosynthesis of consumable compounds, including free fatty acids (FFAs), which are valuable and versatile products that can be utilized as feedstocks in the production of other commodities, such as pharmaceuticals and dietary supplements. To improve the FFA secretory productivity of in the presence of Triton X-100, we analyzed the gene expression of a wild-type control strain and a disruptant strain of an acyl-CoA synthetase gene, , in a time-series experiment. Read More

View Article and Full-Text PDF

Structural diversity of the coenzyme methylofuran and identification of enzymes for the biosynthesis of its polyglutamate side chain.

J Biol Chem 2021 Apr 21:100682. Epub 2021 Apr 21.

ETH Zurich, Institute of Microbiology, Vladimir-Prelog-Weg 1-5/10, 8093 Zurich, Switzerland. Electronic address:

Methylofuran (MYFR) is a formyl-carrying coenzyme essential for the oxidation of formaldehyde in most methylotrophic bacteria. In Methylorubrum extorquens, MYFR contains a large and branched polyglutamate side chain of up to 24 glutamates. These glutamates play an essential role in interfacing the coenzyme with the formyltransferase/hydrolase complex, an enzyme that generates formate. Read More

View Article and Full-Text PDF

Further engineering of R. toruloides for the production of terpenes from lignocellulosic biomass.

Biotechnol Biofuels 2021 Apr 21;14(1):101. Epub 2021 Apr 21.

Department of Energy, Agile BioFoundry, Emeryville, CA, 94608, USA.

Background: Mitigation of climate change requires that new routes for the production of fuels and chemicals be as oil-independent as possible. The microbial conversion of lignocellulosic feedstocks into terpene-based biofuels and bioproducts represents one such route. This work builds upon previous demonstrations that the single-celled carotenogenic basidiomycete, Rhodosporidium toruloides, is a promising host for the production of terpenes from lignocellulosic hydrolysates. Read More

View Article and Full-Text PDF

4R-cembranoid confers neuroprotection against LPS-induced hippocampal inflammation in mice.

J Neuroinflammation 2021 Apr 19;18(1):95. Epub 2021 Apr 19.

Department of Biochemistry, Universidad Central del Caribe School of Medicine, Av. Sta. Juanita, Bayamón, 00960, Puerto Rico.

Background: Chronic brain inflammation has been implicated in the pathogenesis of various neurodegenerative diseases and disorders. For example, overexpression of pro-inflammatory cytokines has been associated with impairments in hippocampal-dependent memory. Lipopolysaccharide (LPS) injection is a widely used model to explore the pathobiology of inflammation. Read More

View Article and Full-Text PDF

GmST1, which encodes a sulfotransferase, confers resistance to soybean mosaic virus strains G2 and G3.

Plant Cell Environ 2021 Apr 18. Epub 2021 Apr 18.

Key Laboratory of Soybean Biology in Chinese Education Ministry (Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry), Northeast Agricultural University, Harbin, China.

Soybean mosaic virus (SMV) is one of the most widespread and devastating viral diseases worldwide. The genetic architecture of qualitative resistance to SMV in soybean remains unclear. Here, the Rsvg2 locus was identified as underlying soybean resistance to SMV by genome-wide association and linkage analyses. Read More

View Article and Full-Text PDF

Identification and functional characterization of a new flavonoid synthase gene MdFLS1 from apple.

Planta 2021 Apr 15;253(5):105. Epub 2021 Apr 15.

School of Pharmacy, Liaocheng University, Liaocheng, Shandong, 250000, People's Republic of China.

Main Conclusion: The flavonoid synthase gene MdFLS1 from apple, which possibly plays an important role in anthocyanin synthesis, accumulates in the purple-red branches of Malus 'Pink spire'. Flavonoid metabolism serves an important function in plant growth and development. In this study, we selected 20 varieties of apple lines, 10 green and ten red branches, from the plant nursery of Qingdao Agriculture Academy. Read More

View Article and Full-Text PDF

Analysis of metabolic network disruption in engineered microbial hosts due to enzyme promiscuity.

Metab Eng Commun 2021 Jun 7;12:e00170. Epub 2021 Mar 7.

Department of Computer Science, Tufts University, Medford, MA, USA.

Increasing understanding of metabolic and regulatory networks underlying microbial physiology has enabled creation of progressively more complex synthetic biological systems for biochemical, biomedical, agricultural, and environmental applications. However, despite best efforts, confounding phenotypes still emerge from unforeseen interplay between biological parts, and the design of robust and modular biological systems remains elusive. Such interactions are difficult to predict when designing synthetic systems and may manifest during experimental testing as inefficiencies that need to be overcome. Read More

View Article and Full-Text PDF

The Natural Alkaloid Berberine Can Reduce the Number of Tolerant Cells.

J Nat Prod 2021 Apr 13;84(4):993-1001. Epub 2021 Apr 13.

Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona 60131, Italy.

The eradication of recurrent (PA) lung infection in cystic fibrosis (CF) patients may be hampered by the development of persistent bacterial forms, which can tolerate antibiotics through efflux pump overexpression. After demonstrating the efflux pump inhibitory effect of the alkaloid berberine on the PA MexXY-OprM efflux pump, in this study, we tested its ability (80/320 μg/mL) to enhance tobramycin (20xMIC/1000xMIC) activity against PA planktonic/biofilm cultures. Preliminary investigations of the involvement of MexY in PA tolerance to tobramycin treatment, performed on the isogenic pair PA K767 (wild type)/K1525 (Δ) growing in planktonic and biofilm cultures, demonstrated that the Δ mutant K1525 produced a lower (100 and 10 000 times, respectively) amount of tolerant cells than that of the wild type. Read More

View Article and Full-Text PDF

Strain Construction for Intracellular Metabolic Pathway Localization in Y. lipolytica.

Methods Mol Biol 2021 ;2307:147-157

Environmental and Molecular Sciences Laboratory, PNNL, Richland, WA, USA.

Pathway localization by fluorophore or epitope tagging can be accomplished through a multi-staged DNA construct and confirmation process, to generate a series of successfully tagged protein targets. Prerequisite conditions for this process in Y. lipolytica are auxotrophic selection (leu2 or ura3), impaired non-homologous end joining by deletion or impairment of ku70, and plasmids or gene pieces for epitope-selection cassette construction. Read More

View Article and Full-Text PDF
January 2021

Site-directed mutagenesis of the succinate dehydrogenase subunits B and D from Corynespora cassiicola reveals different fitness costs and sensitivities to succinate dehydrogenase inhibitors.

Environ Microbiol 2021 Apr 8. Epub 2021 Apr 8.

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Carboxamide fungicides target succinate dehydrogenase (SDH). Recently published monitoring studies have shown that Corynespora cassiicola isolates are resistant to one or several SDH inhibitors (SDHIs) with amino acid substitutions in the SDH B and D subunits. We confirmed, by site-directed mutagenesis of the sdhB and sdhD genes, that each of the mutations identified in the field strains of C. Read More

View Article and Full-Text PDF

Enzymes of Polyphosphate Metabolism in Yeast: Properties, Functions, Practical Significance.

Biochemistry (Mosc) 2021 Jan;86(Suppl 1):S96-S108

Institute of Bioengineering, Federal Scientific Center for Biotechnology, Russian Academy of Sciences, Moscow, 117312, Russia.

Inorganic polyphosphates (polyP) are the linear polymers of orthophosphoric acid varying in the number of phosphate residues linked by the energy-rich phosphoanhydride bonds. PolyP is an essential component in living cells. Knowledge of polyP metabolizing enzymes in eukaryotes is necessary for understanding molecular mechanisms of polyP metabolism in humans and development of new approaches for treating bone and cardiovascular diseases associated with impaired mineral phosphorus metabolism. Read More

View Article and Full-Text PDF
January 2021

Overexpression of Dioxygenase Encoding Gene Accelerates the Phenolic Aldehyde Conversion and Ethanol Fermentability of Zymomonas mobilis.

Appl Biochem Biotechnol 2021 Apr 7. Epub 2021 Apr 7.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.

NADH-dependent reductase enzyme catalyzes the phenolic aldehyde conversion and correspondingly improves the ethanol fermentability of the ethanologenic Zymomonas mobilis. This study constructed the transcriptional landscape of mono/dioxygenase genes in Z. mobilis ZM4 under the stress of the toxic phenolic aldehyde inhibitors of 4-hydroxybenzaldehyde, syringaldehyde, and vanillin. Read More

View Article and Full-Text PDF

Indole Inhibits IncP-1 Conjugation System Mainly Through Promoting and Expression.

Front Microbiol 2021 19;12:628133. Epub 2021 Mar 19.

Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, China.

Indole works as an interspecies signal molecule to regulate multiple physiological activities, like antibiotic resistance, acid resistance, and virulence. However, the effect of indole on conjugation is unknown. Here, with λπ as a donor strain that carries a chromosomally integrated conjugative RP4 plasmid, we explored the effect of indole on conjugation of a mobilizable pUCP24T plasmid imparting gentamycin resistance. Read More

View Article and Full-Text PDF

Analysis of HOX gene expression and the effects of HOXA9 overexpression in fibroblasts derived from keloid lesions and normal skin.

Wound Repair Regen 2021 Apr 3. Epub 2021 Apr 3.

Department of Surgery, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA.

Keloids are fibroproliferative lesions resulting from an abnormal wound healing process due to pathological mechanisms that remain incompletely understood. Keloids tend to occur more frequently in anterior versus posterior body regions (e.g. Read More

View Article and Full-Text PDF

Activation of a Cryptic Manumycin-Type Biosynthetic Gene Cluster of DSM44229 by Series of Genetic Manipulations.

Microorganisms 2021 Mar 8;9(3). Epub 2021 Mar 8.

Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University, Studničkova 7, 12800 Prague, Czech Republic.

(1) Background: Manumycins are small actinomycete polyketides with prominent cancerostatic and immunosuppressive activities via inhibition of various eukaryotic enzymes. Their overall activity towards human cells depends on the structural variability of both their polyketide chains, mainly the upper one. In our genetic screening project to find novel producers of anti-inflammatory manumycins, the strain DSM44229 was identified as containing a novel manumycin-type biosynthetic gene cluster (BGC). Read More

View Article and Full-Text PDF

Improved Yield of Recombinant Protein via Flagella Regulator Deletion in .

Front Microbiol 2021 15;12:655072. Epub 2021 Mar 15.

Department of Chemical and Biological Engineering, Korea University, Seoul, South Korea.

Protein production requires a significant amount of intracellular energy. Eliminating the flagella has been proposed to help improve protein production by reducing energy consumption. In this study, the gene encoding a subunit of FlhC, a master regulator of flagella assembly, was deleted to reduce the expression of flagella-related genes. Read More

View Article and Full-Text PDF