25 results match your criteria overexpression mir‑155

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SOD3 induces a HIF-2α-dependent program in endothelial cells that provides a selective signal for tumor infiltration by T cells.

J Immunother Cancer 2020 06;8(1)

Department of Immunology and Oncology, Centro Nacional de Biotecnología, Madrid, Spain

Background: Tumor-infiltrating lymphocytes (TILs), mainly CD8 cytotoxic T lymphocytes (CTL), are linked to immune-mediated control of human cancers and response to immunotherapy. Tumors have nonetheless developed specific mechanisms that selectively restrict T cell entry into the tumor microenvironment. The extracellular superoxide dismutase (SOD3) is an anti-oxidant enzyme usually downregulated in tumors. Read More

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Metabolic engineering of an acid-tolerant yeast strain for itaconic acid production.

Metab Eng Commun 2020 Jun 11;10:e00124. Epub 2020 Feb 11.

Interdepartmental Microbiology Program, Iowa State University, Ames, USA.

Itaconic acid (IA), or 2-methylenesuccinic acid, has a broad spectrum of applications in the biopolymer industry owing to the presence of one vinyl bond and two acid groups in the structure. Its polymerization can follow a similar mechanism as acrylic acid but additional functionality can be incorporated into the extra beta acid group. Currently, the bio-based production of IA in industry relies on the fermentation of the filamentous fungus . Read More

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Metabolic specialization in itaconic acid production: a tale of two fungi.

Curr Opin Biotechnol 2020 04 2;62:153-159. Epub 2019 Nov 2.

Dutch DNA Biotech BV Padualaan 8, 3584CH Utrecht, the Netherlands.

Some of the oldest and most established industrial biotechnology processes involve the fungal production of organic acids. In these fungi, the transport of metabolites between cellular compartments, and their secretion, is a major factor. In this review we exemplify the importance of both mitochondrial and plasma membrane transporters in the case of itaconic acid production in two very different fungal systems, Aspergillus and Ustilago. Read More

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miRNA-215-5p suppresses the aggressiveness of breast cancer cells by targeting Sox9.

FEBS Open Bio 2019 11 22;9(11):1957-1967. Epub 2019 Oct 22.

Department of Vascular Breast Surgery, People's Hospital Affiliated to Nanchang University, Jiangxi, China.

Several studies have shown that miR-215-5p acts as a tumor suppressor in certain cancers, but its role in the progression and metastasis of breast carcinoma remains incompletely understood. Herein, we prove that miR-215-5p is substantially down-expressed in breast carcinoma as compared with nontumor tissue. Up-regulation of miR-215-5p inhibits the aggressive abilities of breast carcinoma cells in vitro. Read More

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November 2019

Targeted therapies for non-HPV-related head and neck cancer: challenges and opportunities in the context of predictive, preventive, and personalized medicine.

EPMA J 2019 Sep 20;10(3):291-305. Epub 2019 Jul 20.

1Department of Oral Biology and Diagnostic Sciences, The Dental College of Georgia, Augusta University, 1120 15th Street, Augusta, GA 30912 USA.

Head and neck squamous cell carcinoma (HNSCC) develops in the mucosal lining of the upper aerodigestive tract, principally as a result of exposure to carcinogens present in tobacco products and alcohol, with oncogenic papillomaviruses also being recognized as etiological agents in a limited proportion of cases. As such, there is considerable scope for prevention of disease development and progression. However, despite multimodal approaches to treatment, tumor recurrence and metastatic disease are common problems, and clinical outcome is unsatisfactory. Read More

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September 2019

Quercus ilex Extract Ameliorates Acute TNBS-Induced Colitis in Rats.

Planta Med 2019 May 24;85(8):670-677. Epub 2019 Apr 24.

Department of Pharmacology, Faculty of Pharmacy, University of Seville, Seville, Spain.

L. (Fagaceae) is one of the most commonly used plants in folk medicine in the Mediterranean region to treat gastrointestinal disorders. The aim of the present study was to evaluate the effects of a polyphenol extract from mature leaves of on acute 2,4,6-trinitrobenzene sulfonic acid-induced colitis in rats. Read More

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Lipidomic Analysis of α-Synuclein Neurotoxicity Identifies Stearoyl CoA Desaturase as a Target for Parkinson Treatment.

Mol Cell 2019 03 4;73(5):1001-1014.e8. Epub 2018 Dec 4.

Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA; Department of Biology, MIT, Cambridge, MA 02139, USA; HHMI, Department of Biology, MIT, Cambridge, MA 02139, USA.

In Parkinson's disease (PD), α-synuclein (αS) pathologically impacts the brain, a highly lipid-rich organ. We investigated how alterations in αS or lipid/fatty acid homeostasis affect each other. Lipidomic profiling of human αS-expressing yeast revealed increases in oleic acid (OA, 18:1), diglycerides, and triglycerides. Read More

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How to create state-of-the-art genetic model systems: strategies for optimal CRISPR-mediated genome editing.

Nucleic Acids Res 2018 07;46(13):6435-6454

Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht University, The Netherlands.

Model systems with defined genetic modifications are powerful tools for basic research and translational disease modelling. Fortunately, generating state-of-the-art genetic model systems is becoming more accessible to non-geneticists due to advances in genome editing technologies. As a consequence, solely relying on (transient) overexpression of (mutant) effector proteins is no longer recommended since scientific standards increasingly demand genetic modification of endogenous loci. Read More

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miR-148a-3p suppresses epithelial ovarian cancer progression primarily by targeting c-Met.

Oncol Lett 2018 May 23;15(5):6131-6136. Epub 2018 Feb 23.

Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China.

MicroRNAs (miRNAs) are a group of small non-coding RNAs that modulate post-transcriptional gene expression. It has been demonstrated that various miRNAs may be expressed at different levels in different types of tumors. The present study assessed the role of microRNA-148a-3p (miR-148a-3p) in epithelial ovarian cancer (EOC). Read More

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The Effects of the WNT-Signaling Modulators BIO and PKF118-310 on the Chondrogenic Differentiation of Human Mesenchymal Stem Cells.

Int J Mol Sci 2018 Feb 13;19(2). Epub 2018 Feb 13.

Developmental BioEngineering, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede 7500 AE, The Netherlands.

Mesenchymal stem cells (MSCs) are multipotent cells, mainly from bone marrow, and an ideal source of cells in bone and cartilage tissue engineering. A study of the chondrogenic differentiation of MSCs is of particular interest for MSCs-based cartilage regeneration. In this study, we aimed to optimize the conditions for the chrondogenic differentiation of MSCs by regulating WNT signaling using the small molecule WNT inhibitor PKF118-310 and activator BIO. Read More

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February 2018

Expression of Arabidopsis class 1 phytoglobin (AtPgb1) delays death and degradation of the root apical meristem during severe PEG-induced water deficit.

J Exp Bot 2017 Nov;68(20):5653-5668

Department of Plant Science, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada.

Maintenance of a functional root is fundamental to plant survival in response to some abiotic stresses, such as water deficit. In this study, we found that overexpression of Arabidopsis class 1 phytoglobin (AtPgb1) alleviated the growth retardation of polyethylene glycol (PEG)-induced water stress by reducing programmed cell death (PCD) associated with protein folding in the endoplasmic reticulum (ER). This was in contrast to PEG-stressed roots down-regulating AtPgb1 that exhibited extensive PCD and reduced expression of several attenuators of ER stress, including BAX Inhibitor-1 (BI-1). Read More

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November 2017

Phosphoglycerol dihydroceramide, a distinctive ceramide produced by Porphyromonas gingivalis, promotes RANKL-induced osteoclastogenesis by acting on non-muscle myosin II-A (Myh9), an osteoclast cell fusion regulatory factor.

Biochim Biophys Acta Mol Cell Biol Lipids 2017 May 31;1862(5):452-462. Epub 2017 Jan 31.

The Forsyth Institute, Department of Immunology and Infectious Diseases, Cambridge, MA, USA; Department of Immunology and Infectious Diseases, The Forsyth Institute, Cambridge, MA, USA; NOVA Southeastern University College of Dental Medicine, Department of Periodontology, Fort Lauderdale, FL, USA. Electronic address:

Among several virulence factors produced by the periodontal pathogen Porphyromonas gingivalis (Pg), a recently identified novel class of dihydroceramide lipids that contains a long acyl-chain has the potential to play a pathogenic role in periodontitis because of its higher level of tissue penetration compared to other lipid classes produced by Pg. However, the possible impact of Pg ceramides on osteoclastogenesis is largely unknown. In the present study, we report that the phosphoglycerol dihydroceramide (PGDHC) isolated from Pg enhanced osteoclastogenesis in vitro and in vivo. Read More

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The role of p53 in cancer drug resistance and targeted chemotherapy.

Oncotarget 2017 Jan;8(5):8921-8946

Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany.

Cancer has long been a grievous disease complicated by innumerable players aggravating its cure. Many clinical studies demonstrated the prognostic relevance of the tumor suppressor protein p53 for many human tumor types. Overexpression of mutated p53 with reduced or abolished function is often connected to resistance to standard medications, including cisplatin, alkylating agents (temozolomide), anthracyclines, (doxorubicin), antimetabolites (gemcitabine), antiestrogenes (tamoxifen) and EGFR-inhibitors (cetuximab). Read More

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January 2017

The cyclin-dependent kinase inhibitor p27 kip1 regulates radial stem cell quiescence and neurogenesis in the adult hippocampus.

Stem Cells 2015 Jan;33(1):219-29

Unidad de Neurobiología Molecular, Área de Biología Celular y Desarrollo, UFIEC, Instituto de Salud Carlos III, Majadahonda, Spain.

Members of the cyclin-dependent kinase (CDK)-inhibitory protein (CIP)/kinase-inhibitory protein (KIP) family of cyclin-dependent kinase inhibitors regulate proliferation and cell cycle exit of mammalian cells. In the adult brain, the CIP/KIP protein p27(kip1) has been related to the regulation of intermediate progenitor cells located in neurogenic niches. Here, we uncover a novel function of p27(kip1) in the adult hippocampus as a dual regulator of stem cell quiescence and of cell-cycle exit of immature neurons. Read More

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January 2015

Overexpression of hsa-miR-148a promotes cartilage production and inhibits cartilage degradation by osteoarthritic chondrocytes.

Osteoarthritis Cartilage 2014 Jan 21;22(1):145-53. Epub 2013 Nov 21.

Department of Orthopaedics, University Medical Center Utrecht, Utrecht, The Netherlands.

Objective: Hsa-miR-148a expression is decreased in Osteoarthritis (OA) cartilage, but its functional role in cartilage has never been studied. Therefore, our aim was to investigate the effects of overexpressing hsa-miR-148a on cartilage metabolism of OA chondrocytes.

Design: OA chondrocytes were transfected with a miRNA precursor for hsa-miR-148a or a miRNA precursor negative control. Read More

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January 2014

Increased expression of the yeast multidrug resistance ABC transporter Pdr18 leads to increased ethanol tolerance and ethanol production in high gravity alcoholic fermentation.

Microb Cell Fact 2012 Jul 27;11:98. Epub 2012 Jul 27.

IBB - Institute for Biotechnology and BioEngineering, Centro de Engenharia Biológica e Química, and Department of Bioengineering, Instituto Superior Técnico, Technical University of Lisbon, Av. Rovisco Pais, 1049-001 Lisbon, Portugal.

Background: The understanding of the molecular basis of yeast tolerance to ethanol may guide the design of rational strategies to increase process performance in industrial alcoholic fermentations. A set of 21 genes encoding multidrug transporters from the ATP-Binding Cassette (ABC) Superfamily and Major Facilitator Superfamily (MFS) in S. cerevisiae were scrutinized for a role in ethanol stress resistance. Read More

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Tissue deformation spatially modulates VEGF signaling and angiogenesis.

Proc Natl Acad Sci U S A 2012 May 17;109(18):6886-91. Epub 2012 Apr 17.

Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, 7500 AE Enschede, The Netherlands.

Physical forces play a major role in the organization of developing tissues. During vascular development, physical forces originating from a fluid phase or from cells pulling on their environment can alter cellular signaling and the behavior of cells. Here, we observe how tissue deformation spatially modulates angiogenic signals and angiogenesis. Read More

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Met-driven invasive growth involves transcriptional regulation of Arhgap12.

Oncogene 2008 Sep 26;27(42):5590-8. Epub 2008 May 26.

Laboratory of Functional Genomics, The Oncogenomics Center, Institute for Cancer Research and Treatment, University of Turin Medical School, Turin, Italy.

Invasive growth is a complex biological program triggered by hepatocyte growth factor (HGF) through its tyrosine kinase receptor encoded by the Met proto-oncogene. The program involves-besides proliferation-cell dissociation, motility and invasiveness, controlled by intracellular signals impinging on PI3K and on the small G-proteins of the Rac/Rho family. The mechanism(s) unbalancing Rac/Rho activation are still not completely clarified. Read More

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September 2008

Erythropoiesis abnormalities contribute to early-onset anemia in patients with septic shock.

Am J Respir Crit Care Med 2006 Jul 30;174(1):51-7. Epub 2006 Mar 30.

Medical Intensive Care Unit, Cochin Hospital, APHP Université Paris-Descartes, 27 rue du Faubourg Saint-Jacques, F-75679 Paris, Cedex 14, France.

Rationale: The intimate mechanisms of early onset anemia observed in critically ill patients with septic shock remain unclear.

Objectives: We investigated erythropoiesis abnormalities in this setting by studying morphologic, functional, and biochemical patterns of erythroid lineage.

Methods: Erythroid lineage in the bone marrow from patients with septic shock who developed early-onset anemia was compared with that of healthy control subjects. Read More

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Bex1, a novel interactor of the p75 neurotrophin receptor, links neurotrophin signaling to the cell cycle.

EMBO J 2006 Mar 23;25(6):1219-30. Epub 2006 Feb 23.

Division of Molecular Neurobiology, Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.

A screening for intracellular interactors of the p75 neurotrophin receptor (p75NTR) identified brain-expressed X-linked 1 (Bex1), a small adaptor-like protein of unknown function. Bex1 levels oscillated during the cell cycle, and preventing the normal cycling and downregulation of Bex1 in PC12 cells sustained cell proliferation under conditions of growth arrest, and inhibited neuronal differentiation in response to nerve growth factor (NGF). Neuronal differentiation of precursors isolated from the brain subventricular zone was also reduced by ectopic Bex1. Read More

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Wnt signalling regulates adult hippocampal neurogenesis.

Nature 2005 Oct;437(7063):1370-5

Laboratory of Genetics, The Salk Institute, La Jolla, California 92037, USA.

The generation of new neurons from neural stem cells is restricted to two regions of the adult mammalian central nervous system: the subventricular zone of the lateral ventricle, and the subgranular zone of the hippocampal dentate gyrus. In both regions, signals provided by the microenvironment regulate the maintenance, proliferation and neuronal fate commitment of the local stem cell population. The identity of these signals is largely unknown. Read More

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October 2005

A role for chemokine receptor transactivation in growth factor signaling.

EMBO Rep 2001 Feb;2(2):151-6

Department of Immunology and Oncology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Cientificias, Universidad Autónoma de Madrid, Spain.

Complex cell responses require the integration of signals delivered through different pathways. We show that insulin-like growth factor (IGF)-I induces specific transactivation of the Gi-coupled chemokine receptor CCR5, triggering its tyrosine phosphorylation and Galpha recruitment. This transactivation occurs via a mechanism involving transcriptional upregulation and secretion of RANTES, the natural CCR5 ligand. Read More

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February 2001

DmPAR-6 directs epithelial polarity and asymmetric cell division of neuroblasts in Drosophila.

Nat Cell Biol 2001 Jan;3(1):43-9

Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A-1030 Vienna, Austria.

The Drosophila protein Bazooka is required for both apical-basal polarity in epithelial cells and directing asymmetric cell division in neuroblasts. Here we show that the PDZ-domain protein DmPAR-6 cooperates with Bazooka for both of these functions. DmPAR-6 colocalizes with Bazooka at the apical cell cortex of epithelial cells and neuroblasts, and binds to Bazooka in vitro. Read More

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January 2001

Autoantibodies against P53 protein in patients with transitional cell carcinoma of the bladder.

Anticancer Res 1999 Jul-Aug;19(4C):3531-7

Laboratorio de Marcadores Tumorales, Hospital General Universitario de Alicante, Spain.

Cellular accumulation and conformational changes of mutant p53 could act as immunogens for auto-antibodies (auto-Abs) generation when altered p53 from tumoral cells reaches the blood stream. Our main objective was to compare the presence and clinical implications of p53-antibodies in serum with the immunohistochemical (IHC) tissue overexpression of protein p53 in patients with transitional cell carcinoma of the bladder, evaluating their association with bladder cancer parameters and their prognostic value. The study comprised 59 patients with bladder cancer (group 1) and 15 healthy controls (group 2). Read More

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January 2000

The matrix metalloproteinase-9 regulates the insulin-like growth factor-triggered autocrine response in DU-145 carcinoma cells.

J Biol Chem 1999 Mar;274(11):6935-45

Department of Immunology and Oncology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Campus de Cantoblanco, E-28049 Madrid, Spain.

The androgen-independent human prostate adenocarcinoma cell line DU-145 proliferates in serum-free medium and produces insulin-like growth factors (IGF)-I, IGF-II, and the IGF type-1 receptor (IGF-1R). They also secrete three IGF-binding proteins (IGFBP), IGFBP-2, -3, and -4. Of these, immunoblot analysis revealed selective proteolysis of IGFBP-3, yielding fragments of 31 and 19 kDa. Read More

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