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Interaction between polyene antifungal drug and saturated phospholipid monolayer regulated by calcium ions at the air-water interface.

Juan Wang Hao Zhu

Colloids Surf B Biointerfaces 2021 Jul 22;207:111998. Epub 2021 Jul 22.

Shaanxi Engineering Research Center of Controllable Neutron Source, School of Science, Xijing University, Xi'an, 710123, China.

Amphotericin B (AmB) is a polyene antifungal drug, which could directly form pores on the sterol-free phospholipid monolayers. The interaction between AmB and phospholipid can be affected by calcium ions, but the mechanism is still unclear. DPPC is a saturated phospholipid with -PC group, which is often used to simulate the outer cell membrane leaflet. Read More

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Disruption of protein rhamnosylation affects the host interaction.

Cell Surf 2021 Dec 26;7:100058. Epub 2021 Jun 26.

Departamento de Biología, División de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta s/n, col. Noria Alta, C.P. 36050 Guanajuato, Gto., Mexico.

Sporotrichosis is a fungal disease caused by the members of the pathogenic clade, and one of the etiological agents is The cell wall of this organism has been previously analyzed and thus far is known to contain an inner layer composed of chitin and β -glucans, and an outer layer of glycoproteins, which are decorated with mannose and rhamnose-containing oligosaccharides. The L-rhamnose biosynthesis pathway is common in bacteria but rare in members of the Fungi kingdom. Therefore, in this study, we aimed to disrupt this metabolic route to assess the contribution of rhamnose during the -host interaction. Read More

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December 2021

Identification and physical characterization of a spontaneous mutation of the tobacco mosaic virus in the laboratory environment.

Sci Rep 2021 Jul 23;11(1):15109. Epub 2021 Jul 23.

Department of Chemistry and Biochemistry, The University of Texas at Dallas, 800 West Campbell Road, Richardson, TX, 75080, USA.

Virus-like particles are an emerging class of nano-biotechnology with the Tobacco Mosaic Virus (TMV) having found a wide range of applications in imaging, drug delivery, and vaccine development. TMV is typically produced in planta, and, as an RNA virus, is highly susceptible to natural mutation that may impact its properties. Over the course of 2 years, from 2018 until 2020, our laboratory followed a spontaneous point mutation in the TMV coat protein-first observed as a 30 Da difference in electrospray ionization mass spectrometry (ESI-MS). Read More

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Analysis of AM Parameters on Surface Roughness Obtained in PLA Parts Printed with FFF Technology.

Polymers (Basel) 2021 Jul 20;13(14). Epub 2021 Jul 20.

Engineering Department, Public University of Navarre (UPNA), Arrosadia Campus, 31006 Pamplona, Spain.

Fused filament fabrication (FFF) 3D printing technology allows very complex parts to be obtained at a relatively low cost and in reduced manufacturing times. In the present work, the effect of main 3D printing parameters on roughness obtained in curved surfaces is addressed. Polylactic acid (PLA) hemispherical cups were printed with a shape similar to that of the acetabular part of the hip prostheses. Read More

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Effect of Flue Gases' Corrosive Components on the Degradation Process of Evaporator Tubes.

Materials (Basel) 2021 Jul 10;14(14). Epub 2021 Jul 10.

Institute of Materials and Quality Engineering, Faculty of Materials, Metallurgy and Recycling, Technical University of Košice, 04200 Košice, Slovakia.

Corrosion of boiler tubes remains an operational and economic limitation in municipal waste power plants. The understanding of the nature, mechanism, and related factors can help reduce the degradation process caused by corrosion. The chlorine content in the fuel has a significant effect on the production of gaseous components (e. Read More

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Role of Extracellular Vimentin in Cancer-Cell Functionality and Its Influence on Cell Monolayer Permeability Changes Induced by SARS-CoV-2 Receptor Binding Domain.

Int J Mol Sci 2021 Jul 12;22(14). Epub 2021 Jul 12.

Faculty of Natural Sciences and Technology, Experimental Physics, Saarland University, 66123 Saarbrücken, Germany.

The cytoskeletal protein vimentin is secreted under various physiological conditions. Extracellular vimentin exists primarily in two forms: attached to the outer cell surface and secreted into the extracellular space. While surface vimentin is involved in processes such as viral infections and cancer progression, secreted vimentin modulates inflammation through reduction of neutrophil infiltration, promotes bacterial elimination in activated macrophages, and supports axonal growth in astrocytes through activation of the IGF-1 receptor. Read More

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Biocompatibility and Biological Performance Evaluation of Additive-Manufactured Bioabsorbable Iron-Based Porous Suture Anchor in a Rabbit Model.

Int J Mol Sci 2021 Jul 8;22(14). Epub 2021 Jul 8.

Department of Orthopedics, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan.

This study evaluated the biocompatibility and biological performance of novel additive-manufactured bioabsorbable iron-based porous suture anchors (iron_SAs). Two types of bioabsorbable iron_SAs, with double- and triple-helical structures (iron_SA_2_helix and iron_SA_3_helix, respectively), were compared with the synthetic polymer-based bioabsorbable suture anchor (polymer_SAs). An in vitro mechanical test, MTT assay, and scanning electron microscope (SEM) analysis were performed. Read More

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Simpson Grade I Resection of Type II Clinoidal Meningiomal Through Cranio-orbital Zygomatic Approach: 2-Dimensional Operative Video.

Oper Neurosurg (Hagerstown) 2021 Jul 22. Epub 2021 Jul 22.

Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.

Clinoidal meningiomas have been considered as a separate entity with distinguishing clinical, radiological, and surgical considerations.1-2 Surgical mortality and morbidity associated with anterior clinoidal meningiomas has remained high in the past, with radical resection considered unattainable.3 However, the extent of surgical removal is clearly the most determining factor in tumor recurrence and progression. Read More

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Prospects for the use of indicators of sialic acid metabolism in medicine (review of literature).

Klin Lab Diagn 2021 Jul;66(7):389-395

Saint-Petersburg State Pediatric Medical University.

Sialic acids (SA) determine the degree of molecular hydrophilia, relieve binding together and their transportation, they increase mucin viscosity, stabilize the protein and membrane structure. Apart from that, SA are structural components of gangliosides participating in the formation of the outer layer of the plasma membrane. The degree of silyliation of glycoproteins and glycolipids is an important factor of molecular recognition in the cell, between the cells, between a cell and territorial matrix, as well as between a cell and some outer pathogenic factors. Read More

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Competition between Osmotic Squeezing versus Friction-Driven Swelling of Gels.

Gels 2021 Jul 14;7(3). Epub 2021 Jul 14.

Graduate School of Environment and Information Sciences, Yokohama National University, Yokohama 2408501, Japan.

Some types of hydro-gels have almost the same equilibrium swelling volume in water and in ethylene glycol (EG), a highly viscous liquid completely miscible with water. Experiments showed that when a gel fully swollen with EG is immersed into a large amount of water, it temporarily swells up and then relaxes to the equilibrium volume in water. The temporary swelling is explained by the friction force exerted on the gel network from the outward EG flux In this paper, we experimentally show that the temporary swelling is suppressed by adding linear PEG (polyethylene glycol) in the outer water. Read More

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Influence of intraoperative closed glove exchange on glove contamination during clean soft tissue surgeries.

Vet Surg 2021 Jul 19. Epub 2021 Jul 19.

Department of Veterinary Medicine and Surgery, University of Missouri College of Veterinary Medicine, Columbia, Missouri, USA.

Objectives: To determine the influence of intraoperative glove exchange on glove contamination during clean soft tissue surgery.

Study Design: Prospective clinical study.

Sample Population: Two hundred pairs of gloves and gowns from 50 clean soft tissue surgeries. Read More

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Three-dimensional non-destructive visualization of teeth enamel microcracks using X-ray micro-computed tomography.

Sci Rep 2021 Jul 20;11(1):14810. Epub 2021 Jul 20.

Laser Research Center, Faculty of Physics, Vilnius University, Vilnius, Lithuania.

Although the topic of tooth fractures has been extensively analyzed in the dental literature, there is still insufficient information about the potential effect of enamel microcracks (EMCs) on the underlying tooth structures. For a precise examination of the extent of the damage to the tooth structure in the area of EMCs, it is necessary to carry out their volumetric [(three-dimensional (3D)] evaluation. The aim of this study was to validate an X-ray micro-computed tomography ([Formula: see text]CT) as a technique suitable for 3D non-destructive visualization and qualitative analysis of teeth EMCs of different severity. Read More

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Biaxial 360-degree scanning LIDAR using a liquid crystal control.

Seiji Nishiwaki

Sci Rep 2021 Jul 20;11(1):14767. Epub 2021 Jul 20.

Technology Division, Panasonic Corporation, 3-1-1 Yagumo-nakamachi, Moriguchi City, Osaka, 570 - 8501, Japan.

Sophisticated non-mechanical technology for LIDARs is needed to realize safe autonomous cars. We have confirmed the operating principle of a non-mechanical LIDAR by combining concentric circular-grating couplers (CGCs) with a coaxially aligned rod lens. Laser light incident vertically on the center of the inner CGC along the center axis of the lens is radiated from the outer CGC and passes through the side surface of the lens. Read More

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Surface architecture of Neisseria meningitidis capsule and outer membrane as revealed by Atomic Force Microscopy.

Res Microbiol 2021 Jul 17:103865. Epub 2021 Jul 17.

Department of Mathematics and Physics "E. De Giorgi", University of Salento, Lecce, Italy.

An extensive morphological analysis of the Neisseria meningitidis cell envelope, including serogroup B capsule and outer membrane, based on atomic force microscopy (AFM) together with mechanical characterization by force spectroscopic measurements, has been carried out. Three meningococcal strains were used: the encapsulated serogroup B strain B1940, and the isogenic mutants B1940 siaD(+C) (lacking capsule), and B1940 cps (lacking both capsule and lipooligosaccharide outer core). regularly structured AFM experiments with the encapsulated strain B1940 provided unprecedented images of the meningococcal capsule, which seems to be characterized by protrusions ("bumps") with the lateral dimensions of about 30 nm. Read More

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Bacterial Quorum Sensing Signals Promote Large-Scale Remodeling of Lipid Membranes.

Langmuir 2021 Jul 20. Epub 2021 Jul 20.

Dept. of Chemical and Biological Engineering, Univ. of Wisconsin-Madison, 1415 Engineering Dr., Madison, Wisconsin 53706, United States.

We report that -acyl-l-homoserine lactones (AHLs), a class of nonionic amphiphiles that common bacteria use as signals to coordinate group behaviors, can promote large-scale remodeling in model lipid membranes. Characterization of supported lipid bilayers (SLBs) of the phospholipid 1,2-dioleoyl--glycero-3-phosphocholine (DOPC) by fluorescence microscopy and quartz crystal microbalance with dissipation (QCM-D) reveals the well-studied AHL signal 3-oxo-C12-AHL and its anionic head group hydrolysis product (3-oxo-C12-HS) to promote the formation of long microtubules that can retract into hemispherical caps on the surface of the bilayer. These transformations are dynamic, reversible, and dependent upon the head group structure. Read More

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Dark-pigmented biodeteriogenic fungi in etruscan hypogeal tombs: New data on their culture-dependent diversity, favouring conditions, and resistance to biocidal treatments.

Fungal Biol 2021 Aug 29;125(8):609-620. Epub 2021 Mar 29.

Dipartimento di Scienze, Università 'Roma Tre', Viale G. Marconi 446 - 00146, Rome, Italy.

Subterranean Cultural Heritage sites are frequently subject to biological colonization due to the high levels of humidity, even in conditions of low irradiance and oligotrophy. Here microorganisms form complex communities that may be dangerous through mineral precipitation, through the softening of materials or causing frequent surface discolorations. A reduction of contamination's sources along with the control of microclimatic conditions and biocide treatments (overall performed with benzalkonium chloride) are necessary to reduce microbial growths. Read More

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The nanotube express: Delivering a stapled peptide to the cell surface.

J Colloid Interface Sci 2021 Jul 8;604:670-679. Epub 2021 Jul 8.

Bioinformatics Institute (A*STAR), 30 Biopolis Str., #07-01 Matrix, 38671 Singapore, Singapore; National University of Singapore, Department of Biological Sciences, 14 Science Drive 4, 117543 Singapore, Singapore. Electronic address:

Hypothesis: Carbon nanotubes (CNTs) represent a novel platform for cellular delivery of therapeutic peptides. Chemically-functionalized CNTs may enhance peptide uptake by improving their membrane targeting properties.

Experiments: Using coarse-grained (CG) molecular dynamics (MD) simulations, we investigate membrane interactions of a peptide conjugated to pristine and chemically-modified CNTs. Read More

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New method for the protection and restoration of calcareous cultural heritage stones by polyelectrolytes and hydroxyapatite nanocrystals.

J Colloid Interface Sci 2021 Jul 6;604:604-615. Epub 2021 Jul 6.

Climate and Atmosphere Research Center, The Cyprus Institute, Nicosia 2121, Cyprus; Faculty of Civil Engineering and Geosciences, Delft University of Technology, Delft 2628 CN, the Netherlands.

We have investigated the feasibility of a new two-step protocol for the restoration of marbles. The process employs a polyelectrolyte multilayer film that enhances the chemical affinity between the treated stone and restorative material (hydroxyapatite nanocrystals), through functionalization, while at the same time it attributes an acid resistant property to the resulting system. Surface functionalization and material deposition is achieved through spraying; a simple and versatile application method suitable for objects of various sizes and geometries. Read More

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Titanium Functionalized with Polylysine Homopolymers: In Vitro Enhancement of Cells Growth.

Materials (Basel) 2021 Jul 3;14(13). Epub 2021 Jul 3.

Department of Precision Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, Italy.

In oral implantology, the success and persistence of dental implants over time are guaranteed by the bone formation around the implant fixture and by the integrity of the peri-implant mucosa seal, which adheres to the abutment and becomes a barrier that hinders bacterial penetration and colonization close to the outer parts of the implant. Research is constantly engaged in looking for substances to coat the titanium surface that guarantees the formation and persistence of the peri-implant bone, as well as the integrity of the mucous perimeter surrounding the implant crown. The present study aimed to evaluate in vitro the effects of a titanium surface coated with polylysine homopolymers on the cell growth of dental pulp stem cells and keratinocytes to establish the potential clinical application. Read More

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Residual periodontal pocket treatment with Er:YAG laser-assisted comprehensive periodontal pocket therapy: a retrospective study.

Clin Oral Investig 2021 Jul 19. Epub 2021 Jul 19.

Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.

Objectives: Recently, the application of erbium-doped yttrium aluminum garnet (Er:YAG) laser has been increasing in periodontal therapy. In this retrospective study, we evaluated the safety and effectiveness of a novel pocket therapy using Er:YAG laser in combination with conventional mechanical scaling and root planing treatment (Er:YAG laser-assisted comprehensive periodontal pocket therapy).

Methods: Forty sites in 29 elderly patients having residual periodontal pockets of ≥ 5 mm depth were treated by curette and Er:YAG laser from 2006 to 2009. Read More

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Ground-State Structures of Hydrated Calcium Ion Clusters From Comprehensive Genetic Algorithm Search.

Front Chem 2021 30;9:637750. Epub 2021 Jun 30.

Key Laboratory of Materials Modification by Laser, Ion and Electron Beams, Ministry of Education, Dalian University of Technology, Dalian, China.

We searched the lowest-energy structures of hydrated calcium ion clusters Ca(HO) ( = 10-18) in the whole potential energy surface by the comprehensive genetic algorithm (CGA). The lowest-energy structures of Ca(HO) clusters show that Ca is always surrounded by six HO molecules in the first shell. The number of first-shell water molecules changes from six to eight at = 12. Read More

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Comparison of Antibacterial Mode of Action of Silver Ions and Silver Nanoformulations With Different Physico-Chemical Properties: Experimental and Computational Studies.

Front Microbiol 2021 1;12:659614. Epub 2021 Jul 1.

Department of Microbiology, Faculty of Biological Sciences, University of Wrocław, Wrocław, Poland.

The aim of this study was to compare the antibacterial mode of action of silver ions (Ag) and selected silver nanoformulations against strains ( J53, BW25113 and its derivatives: Δ A, Δ C, Δ F, Δ R, ompRG596AcusSG1130A, cusSG1130A). In this research we used various experimental methods and techniques such as determination of the minimal inhibitory concentration, flow cytometry, scanning electron microscopy, circular dichroism as well as computational methods of theoretical chemistry. Thanks to the processing of bacteria and silver samples (ions and nanoformulations), we were able to determine the bacterial sensitivity to silver samples, detect reactive oxygen species (ROS) in the bacterial cells, visualize the interaction of silver samples with the bacterial cells, and identify their interactions with proteins. Read More

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Tanshinones from Salvia miltiorrhiza inhibit Mycobacterium tuberculosis via disruption of the cell envelope surface and oxidative stress.

Food Chem Toxicol 2021 Jul 14:112405. Epub 2021 Jul 14.

Bulgarian Academy of Sciences, The Stephan Angeloff Institute of Microbiology, Laboratory of Metabolomics, Plovdiv, Bulgaria; Center of Plant Systems Biology and Biotechnology, Plovdiv, Bulgaria. Electronic address:

The unique structure of Mycobacterium tuberculosis cell envelope provides impermeable barrier against environmental stimuli. In the situation that this barrier is disturbed Mycobacteria react at the transcriptional and translational level to redirect metabolic processes and to maintain integrity of the cell. In this work we aimed to explore the early metabolic response of M. Read More

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Biomacromolecules of chitosan - Bacopa saponin based LipL32 gene delivery system for leptospirosis therapy.

Environ Res 2021 Jul 15;202:111699. Epub 2021 Jul 15.

School of Renewable Energy, Maejo University, Chiang Mai, 50290, Thailand; College of Medical and Health Science, Asia University, Taichung, Taiwan. Electronic address:

Leptospirosis is a severe bacterial infectious disease caused by the organisms belonging to the genus of Leptospira. The chitosan/Bacopa saponin/tripolyphosphate (CS/BS/TPP) nanoparticles conjugated with recombinant DNA vaccines were designed against Leptospirosis. Chitosan, a polysaccharide is suitable for delivery of drug, and gene due to its bio-compatible and biodegradable properties. Read More

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The effect of anthropogenic activities on the environmental fate of chlorinated paraffins in surface soil in an urbanized zone of northern China.

Environ Pollut 2021 Jul 10;288:117766. Epub 2021 Jul 10.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.

Chlorinated paraffins (CPs) have been widely used as halogenated flame retardants and plasticizers since the mid-20th century. The prevalence of CPs in soil has been widely reported, but the distribution pattern of CPs in urbanized zones and their association with multiple socioeconomic variables have not been adequately explored. Herein, short-chain and medium-chain chlorinated paraffins (SCCPs and MCCPs) were investigated in surface soil samples from Tianjin, China, a typical urbanized area. Read More

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Influences of the bark economics spectrum and positive termite feedback on bark and xylem decomposition.

Ecology 2021 Jul 16:e03480. Epub 2021 Jul 16.

Department of Ecological Science, Faculty of Science, Vrije Universiteit Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands.

The plant economics spectrum integrates trade-offs and covariation in resource economic traits of different plant organs and their consequences for pivotal ecosystem processes, such as decomposition. However, in this concept stems are often considered as one unit ignoring the important functional differences between wood (xylem) and bark. These differences may not only affect the performance of woody plants during their lifetime, but may also have important "afterlife effects". Read More

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Enhancement of Scattering and Near Field of TiO-Au Nanohybrids Using a Silver Resonator for Efficient Plasmonic Photocatalysis.

ACS Appl Mater Interfaces 2021 Jul 16. Epub 2021 Jul 16.

Institute of Quantum and Sustainable Technology (IQST), School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.

Recently, localized surface plasmon resonances (SPRs) of metallic nanoparticles (NPs) have been widely used to construct plasmonic nanohybrids for heterogeneous photocatalysis. For example, the combination of plasmonic Au NPs and TiO provides pure TiO visible-light activity. The SPR effect induces an electric field and consequently enhances light scattering and absorption, favoring the transfer of photon energy to hot carriers for catalytic reactions. Read More

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Calixarene-decorated liposomes for intracellular cargo delivery.

Org Biomol Chem 2021 Jul 16. Epub 2021 Jul 16.

Department of Chemistry, Life Sciences and Environmental Sustainability, Università degli Studi di Parma, Parco Area delle Scienze 17/A, 43124 Parma, Italy.

Amphiphilic calix[4]arenes, functionalized with guanidinium groups, are used to decorate the outer surface of liposomes and significantly improve the cellular uptake of a cargo compared to plain liposomes. The improved uptake is elicited and mediated by the interaction between the cationic polar heads of the macrocycle units embedded in the liposome bilayer and anionic heparan-sulfate proteoglycans surrounding the exterior of cells. Read More

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Ionization properties of monophosphoinositides in mixed model membranes.

Biochim Biophys Acta Biomembr 2021 Jul 12;1863(11):183692. Epub 2021 Jul 12.

Department of Biological Sciences, Kent State University, PO Box 5190, Kent, OH 44242, USA. Electronic address:

Phosphoinositides are found in low concentration in cellular membranes but perform numerous functions such as signaling, membrane trafficking, protein recruitment and modulation of protein activity. Spatiotemporal regulation by enzymes that phosphorylate and dephosphorylate the inositol ring results in the production of seven distinct and functionally diverse derivatives. Ionization properties of the phosphorylated headgroups of anionic lipids have been shown to impact how they interact with proteins and lipids in the membrane. Read More

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