43 results match your criteria osteoblasts extents

Staphylococcus aureus ST228 and ST239 as models for expression studies of diverse markers during osteoblast infection and persistence.

Microbiologyopen 2021 03;10(2):e1178

Department of Biomedical and Biotechnological Sciences (BIOMETEC), Medical Molecular Microbiology and Antibiotic Resistance laboratory (MMARLab, University of Catania, Catania, Italy.

The ability of S. aureus to infect bone and osteoblasts is correlated with its incredible virulence armamentarium that can mediate the invasion/internalization process, cytotoxicity, membrane damage, and intracellular persistence. We comparatively analyzed the interaction, persistence, and modulation of expression of selected genes and cell viability in an ex vivo model using human MG-63 osteoblasts of two previously studied and well-characterized S. Read More

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Evaluation of the cytocompatibility of methacrylate resin-based root canal sealers with osteoblast-like cells.

Dent Mater J 2021 Mar 13. Epub 2021 Mar 13.

Department of Pulp Biology and Endodontics, Division of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU).

This study compared the cytocompatibilities of three methacrylate resin-based root canal sealers [MetaSEAL Soft (MSS), Hybrid Root SEAL (HRS), and Superbond Sealer (SBS)] in either freshly mixed or set conditions using the Kusa A1 osteoblastic cell line. The three sealers and an epoxy resin-based sealer (AH Plus; AHP) were extracted in culture medium; cell growth and osteogenic properties were analyzed. Cell adhesion on set sealers was analyzed with scanning electron microscopy. Read More

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Marine-Inspired Enzymatic Mineralization of Dairy-Derived Whey Protein Isolate (WPI) Hydrogels for Bone Tissue Regeneration.

Mar Drugs 2020 Jun 2;18(6). Epub 2020 Jun 2.

Engineering Department, Lancaster University, Lancaster LA1 4YW, UK.

Whey protein isolate (WPI) is a by-product from the production of cheese and Greek yoghurt comprising β-lactoglobulin (β-lg) (75%). Hydrogels can be produced from WPI solutions through heating; hydrogels can be sterilized by autoclaving. WPI hydrogels have shown cytocompatibility and ability to enhance proliferation and osteogenic differentiation of bone-forming cells. Read More

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Mechanical Regulation of the Maternal Skeleton during Reproduction and Lactation.

Curr Osteoporos Rep 2019 12;17(6):375-386

School of Life Sciences, University of Science and Technology of China, Hefei, China.

Purpose Of Review: This review summarizes recently published data on the effects of pregnancy and lactation on bone structure, mechanical properties, and mechano-responsiveness in an effort to elucidate how the balance between the structural and metabolic functions of the skeleton is achieved during these physiological processes.

Recent Findings: While pregnancy and lactation induce significant changes in bone density and structure to provide calcium for fetal/infant growth, the maternal physiology also comprises several innate compensatory mechanisms that allow for the maintenance of skeletal mechanical integrity. Both clinical and animal studies suggest that pregnancy and lactation lead to adaptations in cortical bone structure to allow for rapid calcium release from the trabecular compartment while maintaining whole bone stiffness and strength. Read More

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December 2019

Osteocytes respond to particles of clinically-relevant conventional and cross-linked polyethylene and metal alloys by up-regulation of resorptive and inflammatory pathways.

Acta Biomater 2019 03 25;87:296-306. Epub 2019 Jan 25.

Biomedical Orthopaedic Research Group, Centre for Orthopaedic & Trauma Research, The University of Adelaide, Adelaide, SA 5000, Australia. Electronic address:

Periprosthetic osteolysis is a major cause of implant failure in total hip replacements. Aseptic loosening caused by osteolytic lesions is associated with the production of bioactive wear particles from the articulations of implants. Wear particles infiltrate the surrounding tissue of implants, promoting inflammation as well as bone resorption. Read More

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Differential effects of type 1 diabetes mellitus and subsequent osteoblastic β-catenin activation on trabecular and cortical bone in a mouse model.

Exp Mol Med 2018 12 5;50(12):1-14. Epub 2018 Dec 5.

State Key Laboratory of Trauma, Burn and Combined Injury, Department of War Wound Rescue Skills Training, Base of Army Health Service Training, Army Medical University, 400038, ChongQing, China.

Type 1 diabetes mellitus (T1DM) is a pathological condition associated with osteopenia. WNT/β-catenin signaling is implicated in this process. Trabecular and cortical bone respond differently to WNT/β-catenin signaling in healthy mice. Read More

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December 2018

Directing osteogenic differentiation of BMSCs by cell-secreted decellularized extracellular matrixes from different cell types.

J Mater Chem B 2018 Dec 26;6(45):7471-7485. Epub 2018 Oct 26.

State Key Laboratory of Organic-Inorganic Composites and Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, P. R. China.

Cell-secreted decellularized extracellular matrixes (D-ECM) are promising for conferring bioactivity and directing cell fate to facilitate tissue regeneration. A cell sheet is a good shape for obtaining D-ECM after decellularization. In this study, cell sheets derived from bone marrow mesenchymal stem cells (BMSCs), MC3T3 osteoblasts, and L929 fibroblasts were decellularized, the three types of D-ECMs obtained were investigated for their capabilities in inducing osteogenic differentiation of re-seeded BMSCs. Read More

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December 2018

Evaluation of free or anchored antimicrobial peptides as candidates for the prevention of orthopaedic device-related infections.

J Pept Sci 2017 Oct 14;23(10):777-789. Epub 2017 Jul 14.

Department of Medicine, University of Udine, P.le Kolbe 4, 33100, Udine, Italy.

The prevention of implant-associated infection, one the most feared complications in orthopaedic surgery, remains a major clinical challenge and urges development of effective methods to prevent bacterial colonization of implanted devices. Alpha-helical antimicrobial peptides (AMPs) may be promising candidates in this respect due to their potent and broad-spectrum antimicrobial activity, their low tendency to elicit resistance and possible retention of efficacy in the immobilized state. The aim of this study was to evaluate the potential of five different helical AMPs, the cathelicidins BMAP-27 and BMAP-28, their (1-18) fragments and the rationally designed, artificial P19(9/G7) peptide, for the prevention of orthopaedic implant infections. Read More

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October 2017

Nonmonotonic Self-Deformation of Cell Nuclei on Topological Surfaces with Micropillar Array.

ACS Appl Mater Interfaces 2017 Jun 25;9(22):18521-18530. Epub 2017 May 25.

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University , Shanghai 200433, China.

Cells respond to the mechanical signals from their surroundings and integrate physiochemical signals to initiate intricate mechanochemical processes. While many studies indicate that topological features of biomaterials impact cellular behaviors profoundly, little research has focused on the nuclear response to a mechanical force generated by a topological surface. Here, we fabricated a polymeric micropillar array with an appropriate dimension to induce a severe self-deformation of cell nuclei and investigated how the nuclear shape changed over time. Read More

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Hypomorphic conditional deletion of E11/Podoplanin reveals a role in osteocyte dendrite elongation.

J Cell Physiol 2017 Nov 14;232(11):3006-3019. Epub 2017 Jun 14.

Roslin Institute and R(D)SVS, The University of Edinburgh, Easter Bush, Midlothian, UK.

The transmembrane glycoprotein E11/Podoplanin (Pdpn) has been implicated in the initial stages of osteocyte differentiation. However, its precise function and regulatory mechanisms are still unknown. Due to the known embryonic lethality induced by global Pdpn deletion, we have herein explored the effect of bone-specific Pdpn knockdown on osteocyte form and function in the post-natal mouse. Read More

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November 2017

Evaluation of cell-surface interaction using a 3D spheroid cell culture model on artificial extracellular matrices.

Mater Sci Eng C Mater Biol Appl 2017 Apr 20;73:310-318. Epub 2016 Dec 20.

Institute of Materials Science, Max Bergmann Center of Biomaterials, TU Dresden, Budapester Straße 27, 01069 Dresden, Germany.

Since decades, cell-surface interactions are studied in 2D cell culture approaches, but cells organized in 3D (spheroids) reflect the normal situation of cells in tissues much better due to intense cell-cell-contacts. Accordingly, this study aimed to prove, if spheroids could be used to study cell-surface interaction. Spheroids consisting of fibroblasts and/or osteoblasts were seeded on artificial extracellular matrices. Read More

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Osteoblast Differentiation and Bone Matrix Formation In Vivo and In Vitro.

Tissue Eng Part B Rev 2017 06 27;23(3):268-280. Epub 2016 Dec 27.

8 Department of Neurobiology, Pharmacology & Physiology, University of Chicago , Chicago, Illinois.

We review the characteristics of osteoblast differentiation and bone matrix synthesis. Bone in air breathing vertebrates is a specialized tissue that developmentally replaces simpler solid tissues, usually cartilage. Bone is a living organ bounded by a layer of osteoblasts that, because of transport and compartmentalization requirements, produce bone matrix exclusively as an organized tight epithelium. Read More

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Visual reporters for study of the osteoblast lineage.

Bone 2016 11 8;92:189-195. Epub 2016 Sep 8.

Department of Reconstructive Sciences, University of Connecticut Health Center, Farmington, CT 06030, USA; Department of Pathophysiology, University of Osijek, Osijek, Croatia. Electronic address:

Advancing our understanding of osteoblast biology and differentiation is critical to elucidate the pathological mechanisms responsible for skeletal diseases such as osteoporosis. Histology and histomorphometry, the classical methods to study osteoblast biology, identify osteoblasts based on their location and morphology and ability to mineralize matrix, but do not clearly define their stage of differentiation. Introduction of visual transgenes into the cells of osteoblast lineage has revolutionized the field and resulted in a paradigm shift that allowed for specific identification and isolation of subpopulations within the osteoblast lineage. Read More

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November 2016

An Examination of Differences in the New Bone Formation Promoted by Different Doses of Recombinant Human Parathyroid Hormone during Mandibular Distraction Osteogenesis.

Plast Reconstr Surg 2016 Feb;137(2):347e-354e

Guiyang, People's Republic of China From the Departments of Oral and Maxillofacial Surgery and Oral Medicine, Affiliated Hospital of Guizhou Medical University.

Background: The administration of different doses of parathyroid hormone to promote mandibular distraction osteogenesis remains unclear. The objective of the present study was to examine the effects of recombinant human parathyroid hormone on new bone formation during mandibular distraction osteogenesis and to investigate the dose-effect relationship associated with this phenomenon.

Methods: A total of 45 rabbits were used to establish the mandibular distraction osteogenesis model. Read More

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February 2016

Reinforcement of injectable calcium phosphate cement by gelatinized starches.

J Biomed Mater Res B Appl Biomater 2016 Apr 7;104(3):615-25. Epub 2015 May 7.

Department of Orthopaedics, Orthopaedic Institute, The First Affiliated Hospital, Soochow University, Suzhou, Jiangsu, 215006, People's Republic of China.

Current injectable calcium phosphate bone cements (CPC) encounter the problems of low strength, high brittleness, and low cohesion in aqueous environment, which greatly hinder their clinical applications for loading-bearing bone substitution and minimally invasive orthopedic surgeries. Here, a strategy of using gelatinized starches to reinforce injectable CPC was investigated. Four types of starches, namely corn starch, crosslinked starch, cationic starch, and Ca-modified starch, were studied for their influence on CPC mechanical properties, injectability, setting times, anticollapsibility, and cytocompatibility. Read More

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Suppression of autophagy in osteocytes does not modify the adverse effects of glucocorticoids on cortical bone.

Bone 2015 Jun 17;75:18-26. Epub 2015 Feb 17.

Center for Osteoporosis and Metabolic Bone Diseases, University of Arkansas for Medical Sciences, Little Rock, AR, USA; Central Arkansas Veterans Healthcare System, Little Rock, AR, USA. Electronic address:

Glucocorticoid excess decreases bone mass and strength in part by acting directly on osteoblasts and osteocytes, but the mechanisms remain unclear. Macroautophagy (herein referred to as autophagy) is a lysosome-based recycling pathway that promotes the turnover of intracellular components and can promote cell function and survival under stressful conditions. Recent studies have shown that glucocorticoids stimulate autophagy in osteocytes, suggesting that autophagy may oppose the negative actions of glucocorticoids on this cell type. Read More

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Malignant phyllodes tumors display mesenchymal stem cell features and aldehyde dehydrogenase/disialoganglioside identify their tumor stem cells.

Breast Cancer Res 2014 Mar 26;16(2):R29. Epub 2014 Mar 26.

Introduction: Although breast phyllodes tumors are rare, there is no effective therapy other than surgery. Little is known about their tumor biology. A malignant phyllodes tumor contains heterologous stromal elements, and can transform into rhabdomyosarcoma, liposarcoma and osteosarcoma. Read More

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A bone substitute with high affinity for vitamin D-binding protein--relationship with niche of osteoclasts.

J Cell Mol Med 2014 Jan 28;18(1):170-80. Epub 2013 Nov 28.

Department of Oral Pathology and Bone Metabolism, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.

The biological activity of osteoblasts and osteoclasts is regulated not only by hormones but also by local growth factors, which are expressed in neighbouring cells or included in bone matrix. Previously, we developed hydroxyapatite (HA) composed of rod-shaped particles using applied hydrothermal methods (HHA), and it revealed mild biodegradability and potent osteoclast homing activity. Here, we compared serum proteins adsorbed to HHA with those adsorbed to conventional HA composed of globular-shaped particles (CHA). Read More

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January 2014

The effects of pulsed electromagnetic field on the functions of osteoblasts on implant surfaces with different topographies.

Acta Biomater 2014 Feb 17;10(2):975-85. Epub 2013 Oct 17.

Department of Prosthetic Dentistry, School of Stomatology, Fourth Military Medical University, 169 West Changle Road, Xi'an 710032, China. Electronic address:

The use of pulsed electromagnetic fields (PEMFs) is a promising approach to promote osteogenesis. However, few studies have reported the effects of this technique on the osseointegration of endosseous implants, especially with regard to different implant topographies. We focused on how the initial interaction between cells and the titanium surface is enhanced by a PEMF and the possible regulatory mechanisms in this study. Read More

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February 2014

Effects of surface molecular chirality on adhesion and differentiation of stem cells.

Biomaterials 2013 Dec 24;34(36):9001-9. Epub 2013 Aug 24.

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Advanced Materials Laboratory, Fudan University, Shanghai 200433, China.

Chirality is one of the most fascinating and ubiquitous cues in nature, especially in life. The effects of chiral surfaces on stem cells have, however, not yet been revealed. Herein we examined the molecular chirality effect on stem cell behaviors. Read More

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December 2013

Cytocompatibility evaluation of microwave sintered biphasic calcium phosphate scaffolds synthesized using pH control.

Mater Sci Eng C Mater Biol Appl 2013 Apr 31;33(3):1710-9. Epub 2012 Dec 31.

Department of Biomedical Engineering, Colleges of Engineering and Medicine, University of Toledo, 2801W. Bancroft Street, 5051 Nitschke Hall MS 303, Toledo, OH 43606, USA.

Compounds belonging to the calcium phosphate (CaP) system are known to be major constituents of bone and are bioactive to different extents in vitro and in vivo. Their chemical similarity makes them prime candidates for implants and bone tissue engineering scaffolds. CaP nanoparticles of amorphous hydroxyapatite (aHA) and dicalcium phosphate dihydrate (DCPD) were synthesized using chemical precipitation. Read More

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Neuropeptides stimulate human osteoblast activity and promote gap junctional intercellular communication.

Neuropeptides 2013 Jun 4;47(3):179-86. Epub 2013 Feb 4.

Department of Orthopedic Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, 050051 Hebei Province, China.

Neuropeptides released from the skeletal nerve fibers have neurotransmitter and immunoregulatory roles; they exert paracrine biological effects on bone cells present close to the nerve endings expressing these signaling molecules. The aims of this study were a systematic investigation of the effects of the neuropeptides substance P (SP), calcitonin gene-related peptide (CGRP), vasoactive intestinal polypeptide (VIP), Neuropeptide Y (NPY) and tyrosine hydroxylase (TH) on the cell viability and function of the human osteoblasts, and comparing their difference in the role of regulating bone formation. Cultures of normal human osteoblasts were treated with SP, CGRP, VIP, NPY or TH at three concentrations. Read More

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Evaluation of multifunctional polysaccharide hydrogels with varying stiffness for bone tissue engineering.

Tissue Eng Part A 2013 Nov 11;19(21-22):2452-63. Epub 2013 Jul 11.

Department of Biomedical Engineering, Rensselaer Polytechnic Institute , Troy, New York.

The use of hydrogels for bone regeneration has been limited due to their inherent low modulus to support cell adhesion and proliferation as well as their susceptibility to bacterial infections at the wound site. To overcome these limitations, we evaluated multifunctional polysaccharide hydrogels of varying stiffness to obtain the optimum stiffness at which the gels (1) induce proliferation of human dermal fibroblasts, human umbilical vascular endothelial cells (HUVECs), and murine preosteoblasts (MC3T3-E1), (2) induce osteoblast differentiation and mineralization, and (3) exhibit an antibacterial activity. Rheological studies demonstrated that the stiffness of hydrogels made of a polysaccharide blend of methylcellulose, chitosan, and agarose was increased by crosslinking the chitosan component to different extents with increasing amounts of genipin. Read More

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November 2013

Characteristics and cytocompatibility of biodegradable polymer film on magnesium by spin coating.

Colloids Surf B Biointerfaces 2012 May 21;93:67-74. Epub 2011 Dec 21.

Biomaterials Unit, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), Tsukuba, Ibaraki, Japan.

In recent years, magnesium and its alloys have been investigated as biodegradable metallic materials in cardiovascular stents and bone implants. However, rapid corrosion rate in the early stage of the degradation process greatly influences the cytocompatibility and hinters their application. In this research, biodegradable polymer films are prepared under same coating condition by spin coating in order to improve the early corrosion resistance and cytocompatibility of Mg. Read More

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Arterial calcification: cardiovascular function and clinical outcome.

G M London

Nefrologia 2011 ;31(6):644-7

INSERM U970, Paris and Hopital F.H. Manhès, Fleury-Mérogis, France.

Arterial calcification (AC) is a common complication of CKD and ESRD, and the extents of AC are predictive of subsequent cardiovascular mortality beyond established conventional risk factors. AC develop in two distinct sites: the intima and media layers of the large and medium-sized arterial wall. These two forms are frequently associated. Read More

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Effects of lactic acid and glycolic acid on human osteoblasts: a way to understand PLGA involvement in PLGA/calcium phosphate composite failure.

J Orthop Res 2012 Jun 21;30(6):864-71. Epub 2011 Nov 21.

Orthopaedic Research Unit, University of Cambridge, Cambridge, United Kingdom.

The use of degradable composite materials in orthopedics remains a field of intense research due to their ability to support new bone formation and degrade in a controlled manner, broadening their use for orthopedic applications. Poly (lactide-co-glycolide) acid (PLGA), a degradable biopolymer, is now a popular material for different orthopedic applications and is proposed for use in tissue engineering scaffolds either alone or combined with bioactive ceramics. Interference screws composed of calcium phosphates and PLGA are readily available in the market. Read More

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Polyphenols, isothiocyanates, and carotenoid derivatives enhance estrogenic activity in bone cells but inhibit it in breast cancer cells.

Am J Physiol Endocrinol Metab 2012 Oct 30;303(7):E815-24. Epub 2011 Aug 30.

Dept. of Clinical Biochemistry, Faculty of Health Sciences, Ben-Gurion Univ. of the Negev, PO Box 653, Beer Sheva, Israel.

While exposure to estrogens is a major risk factor of breast and endometrial cancer, it well established that estrogens are beneficial for bone health. We have previously shown that carotenoids inhibit estrogen signaling in breast and endometrial cancer cells. The aim of this study was to compare the effects of various phytonutrients, (carotenoid derivatives, polyphenols, isothiocyanates) on estrogenic activity in breast cancer cells and osteoblast-like cells. Read More

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October 2012

Effect of cell anisotropy on differentiation of stem cells on micropatterned surfaces through the controlled single cell adhesion.

Biomaterials 2011 Nov 31;32(32):8048-57. Epub 2011 Jul 31.

Key Laboratory of Molecular Engineering of Polymers of Ministry of Education, Department of Macromolecular Science, Advanced Materials Laboratory, Fudan University, Shanghai 200433, China.

Micropatterns of arginine-glycine-aspartic acid (RGD) on poly(ethylene glycol) (PEG) hydrogels were fabricated. Under an appropriate size of microislands on this strong and persistent non-fouling background, single mesenchymal stem cells (MSCs) from rats were well localized, keeping the same adhesive area but different shapes. The cell shapes influenced the differentiation of MSCs, and the osteogenic and adipogenic differentiations exhibited different trends. Read More

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November 2011

Fluoride-induced oxidative stress in three-dimensional culture of OS732 cells and rats.

Biol Trace Elem Res 2011 Oct 23;143(1):446-56. Epub 2010 Oct 23.

Department of Endemic Diseases, Jilin University, 1163 Xinmin Street, Changchun 130021, People's Republic of China.

Exposure to excessive fluoride poses a threat to human health, including increased susceptibility to developing the skeletal fluorosis. Despite its recognized importance as an endemic disease, little is known about how fluoride directly impacts on osteoblasts. We previously reported that fluoride-stimulating monolayer-cultured osteoblast proliferation or inhibiting cell viability depended on fluoride-exposure concentration and period, both accompanied with active oxidative stress. Read More

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October 2011

Quantification of enhanced osteoblastic adhesion to ultraviolet-treated titanium plate.

In Vivo 2010 Jul-Aug;24(4):519-23

Dental Research Center (CIEAO), Faculty of Dentistry, Autonomous University of State of Mexico (UAEM), Toluca, Estado de México, México.

Although the advantage of ultraviolet (UV) irradiation of titanium plates for the attachment of osteoblast is known, the details of the experimental conditions have not been described in previous literature. We established optimal conditions of UV irradiation of titanium plate for the adhesion of mouse osteoblast MC3T3-E1 cells. The viable cell number was determined by MTT method. Read More

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October 2010