25,307 results match your criteria optimized parameters

Mechanical property characterization of partially crystalline Poly-Ether-Ether-Ketone.

J Mech Behav Biomed Mater 2021 Jun 1;121:104600. Epub 2021 Jun 1.

CAS Key Laboratory for Mechanical Behavior and Design of Materials, Department of Modern Mechanics. University of Science and Technology of China, Hefei, Anhui, 230027, China.

The present study investigates the mechanical properties of partially crystalline Poly-Ether-Ether-Ketone between the glass transition and the cold crystallization. Biaxial tension, uniaxial tension, and DMA experiments were conducted to investigate the influence of temperature-induced crystallization on mechanical properties, and three stiffening behaviors are observed. Firstly, a 'U' type mechanical property is observed for all three experiments with first softening and then significant stiffening behavior with increasing temperature. Read More

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Structural white matter alterations in carpal tunnel syndrome: A modified TBSS study.

Brain Res 2021 Jun 8:147558. Epub 2021 Jun 8.

School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China; Center of Rehabilitation Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China. Electronic address:

Functional and structural brain alterations have been noted in carpal tunnel syndrome (CTS), the most common entrapment peripheral neuropathy. Previous studies were mainly focused on somatosensory cortices. However, the changes of white matter diffusion properties in nonsensorimotor cortices remain uninvestigated. Read More

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IMCHGAN: Inductive Matrix Completion with Heterogeneous Graph Attention Networks for Drug-Target Interactions Prediction.

IEEE/ACM Trans Comput Biol Bioinform 2021 Jun 11;PP. Epub 2021 Jun 11.

Computational approaches for prediction of drug-target interactions (DTIs) are highly desired in comparison to traditional biological experiments as its fast and low price. We present a novel Inductive Matrix Completion with Heterogeneous Graph Attention Network approach (IMCHGAN) for predicting DTIs. IMCHGAN first adopts a two-level neural attention mechanism approach to learn drug and target latent feature representations from the DTI heterogeneous network respectively. Read More

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Noninvasive electrocardiographic assessment of ventricular activation and remodeling response to cardiac resynchronization therapy.

Heart Rhythm O2 2021 Feb 12;2(1):12-18. Epub 2021 Jan 12.

School of Biomedical Engineering and Imaging Sciences, King's College London, London, United Kingdom.

Background: Cardiac resynchronization therapy (CRT) produces acute changes in electric resynchronization that can be measured noninvasively with electrocardiographic body surface mapping (ECGi). The relation between baseline acute electrophysiology metrics and their manipulation with CRT and reverse remodeling is unclear.

Objective: To test (ECGi) derived parameters of electrical activation as predictors of volumetric response to CRT. Read More

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February 2021

Hybrid AI-assistive diagnostic model permits rapid TBS classification of cervical liquid-based thin-layer cell smears.

Nat Commun 2021 06 10;12(1):3541. Epub 2021 Jun 10.

Department of Pathology, Guangdong Provincial Women's and Children's Dispensary, Shenzhen, Guangdong Province, PR China.

Technical advancements significantly improve earlier diagnosis of cervical cancer, but accurate diagnosis is still difficult due to various factors. We develop an artificial intelligence assistive diagnostic solution, AIATBS, to improve cervical liquid-based thin-layer cell smear diagnosis according to clinical TBS criteria. We train AIATBS with >81,000 retrospective samples. Read More

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Polymeric membrane-modified voltammetric sensors for lipophilic analytes with nanomolar detection limit: Key parameters influencing the response characteristics.

Anal Chim Acta 2021 Aug 13;1171:338642. Epub 2021 May 13.

Vanderbilt Eye Institute, Vanderbilt University Medical Center, Nashville TN, 37232, USA; Biomedical Engineering, Vanderbilt University Medical Center, Nashville TN, 37232, USA.

Thin plasticized PVC membrane-coated glassy carbon working electrodes have been used for the voltammetric measurement of highly lipophilic, electroactive drugs. Compared to conventional working electrodes, these membrane-coated electrodes exhibit remarkable detection limit and selectivity and are less prone to electrode fouling. The unique performance characteristics of these sensors are related to the large partition coefficient of the analyte in the membrane coating where it is oxidized in a non-aqueous membrane phase. Read More

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Anisotropically Functionalized Aptamer-DNA Nanostructures for Enhanced Cell Proliferation and Target-Specific Adhesion in 3D Cell Cultures.

Biomacromolecules 2021 Jun 10. Epub 2021 Jun 10.

Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.

The development of supramolecular hydrogel scaffolds for the precise positioning of biochemical cues is paramount for applications such as tissue engineering. Nucleic acid engineering allows fabrication of three-dimensional (3D) nanostructures with high variability and nanoscale precision. In this study, aptamers were anisotropically functionalized onto branched DNA nanostructures to control their cell adhesion capability, and their efficiency as biological signal inducers for 3D cell cultivation was investigated. Read More

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Quality control of dietary supplements: An economic green spectrofluorimetric assay of Raspberry ketone and its application to weight variation testing.

Spectrochim Acta A Mol Biomol Spectrosc 2021 May 29;261:120032. Epub 2021 May 29.

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.

Although Dietary supplements are readily accessible and extensively used worldwide, they are inadequately regulated and consumers are victims of manufacturers' fraud. Thus, quality regulations are required to ensure safety of products available to the public. We propose the first native spectrofluorimetric quality control assay of raspberry ketone, a popular dietary supplement ingredient for weight loss. Read More

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Bioleaching of indium from waste LCD panels by Aspergillus niger: Method optimization and mechanism analysis.

Sci Total Environ 2021 May 29;790:148151. Epub 2021 May 29.

School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, PR China.

Using Aspergillus niger (A. niger) to produce low-concentration organic acids is challenging for dissolving InO from waste LCD (liquid crystal display) panels with high toxicity. In this study, three bioleaching approaches from the general and the optimized fermentation systems were investigated respectively to compare indium recovery effects and firstly clarified its bioleaching mechanism. Read More

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Parallel, High-Quality Proteomic and Targeted Metabolomic Quantification Using Laser Capture Microdissected Tissues.

Anal Chem 2021 Jun 10. Epub 2021 Jun 10.

Department of Pharmaceutical Sciences, SUNY-Buffalo, Buffalo, New York 14214, United States.

Quantitative proteomics/metabolomics investigation of laser-capture-microdissection (LCM) cell populations from clinical cohorts affords precise insights into disease/therapeutic mechanisms, nonetheless high-quality quantification remains a prominent challenge. Here, we devised an LC/MS-based approach allowing parallel, robust global-proteomics and targeted-metabolomics quantification from the same LCM samples, using biopsies from prostate cancer (PCa) patients as the model system. The strategy features: (i) an optimized molecular weight cutoff (MWCO) filter-based separation of proteins and small-molecule fractions with high and consistent recoveries; (ii) microscale derivatization and charge-based enrichment for ultrasensitive quantification of key androgens (LOQ = 5 fg/1k cells) with excellent accuracy/precision; (iii) reproducible/precise proteomics quantification with low-missing-data using a detergent-cocktail-based sample preparation and an IonStar pipeline for reproducible and precise protein quantification with excellent data quality. Read More

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Electroanalytical performance of a β-cyclodextrin and ionic liquid modified carbon paste electrode for the determination of verapamil in urine and pharmaceutical formulation.

Anal Methods 2021 Jun 10. Epub 2021 Jun 10.

University of Novi Sad, Faculty of Sciences, Department of Chemistry, Biochemistry and Environmental Protection, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia.

The analytical performance of sensitive and cost-effective electrochemical sensors based on ionic liquids (ILs) with the bis(trifluoromethylsulfonyl)imide anion, [NTf2]-, and the imidazolium cation with different alkyl chain lengths for electrochemical oxidation of verapamil (VER) was investigated. 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([EMIM][NTf2]), 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([BMIM][NTf2]) and 1-hexyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([HMIM][NTf2]) were studied as possible materials for modification of a carbon paste electrode (CPE) for trace-level determination of VER. The experimental parameters including selection of the working electrode, the pH of working media, and the amount of CPE modifiers were investigated. Read More

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L-asparaginase from spp.: effects of production process and biochemical parameters.

Prep Biochem Biotechnol 2021 Jun 10:1-11. Epub 2021 Jun 10.

Department of Food Science, School of Food Engineering, UNICAMP, Campinas, Brazil.

L-asparaginases prevent the formation of acrylamide, a substance commonly found in foods subjected to heat and that also contains reducing sugars and L-asparagine. This work aimed to select a strain of spp. able to produce L-asparaginase and to optimize the fermentation parameters, the partial purification and biochemical characterization were also performed. Read More

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Vortex-assisted dispersive solid phase microextraction using FeO/FeOOH magnetic nanocomposites for high-performance thin-layer chromatographic determination of zolmitriptan in rabbit plasma samples.

J Chromatogr A 2021 May 24;1651:462276. Epub 2021 May 24.

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sphinx University, Assiut 71515, Egypt.

In this work, a fast, versatile, and convenient dispersive solid-phase micro-extraction (DSPME) method is combined with a spectro-densitometric technique for the analysis of zolmitriptan (ZOLM) in biological fluids. FeO/FeOOH magnetic nanocomposites (MNCs) were prepared by a co-precipitation method in aqueous solutions and utilized subsequently as a sorbent in DSPME. By coupling DSPME with high-performance thin-layer chromatography (HPTLC) with fluorescence detection, the preconcentration and determination of (ZOLM) in presence of metoclopramide (MET) and paracetamol (PARA), which are prescribed as an adjuvant therapy with ZOLM, was accomplished. Read More

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Supramolecular Nanosubstrate-Mediated Delivery for CRISPR/Cas9 Gene Disruption and Deletion.

Small 2021 Jun 8:e2100546. Epub 2021 Jun 8.

Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, California NanoSystems Institute (CNSI), Crump Institute for Molecular Imaging (CIMI), University of California, Los Angeles, Los Angeles, CA, 90095, USA.

The clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR/Cas9) is an efficient and precise gene-editing technology that offers a versatile solution for establishing treatments directed at genetic diseases. Currently, CRISPR/Cas9 delivery into cells relies primarily on viral vectors, which suffer from limitations in packaging capacity and safety concerns. These issues with a nonviral delivery strategy are addressed, where Cas9•sgRNA ribonucleoprotein (RNP) complexes can be encapsulated into supramolecular nanoparticles (SMNP) to form RNP⊂SMNPs, which can then be delivered into targeted cells via supramolecular nanosubstrate-mediated delivery. Read More

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High-Yield Preparation of Edge-Functionalized and Water Dispersible Few-Layers of Hexagonal Boron Nitride (hBN) by Direct Wet Chemical Exfoliation.

Nanotechnology 2021 Jun 8. Epub 2021 Jun 8.

University of Adelaide Faculty of Engineering Computer and Mathematical Sciences, Adelaide, South Australia, 5005, AUSTRALIA.

Owing to many fascinating properties including high thermal and chemical stability, excellent electrical insulation, fire-retardant and antibacterial properties, hexagonal boron nitride (hBN) has emerged as a prominent 2D material for broad applications. However, the production of high quality of hBN by chemical exfoliation from its precursor is still challenging. This paper presents a high-yield (+83 %), low cost and energy-efficient wet chemical exfoliation strategy, which produces few-layers (FL, 3-6 layers) of edge-functionalized (OH) hBN nanosheets with uniform size (486±51 nm). Read More

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A New Spike Sorting Algorithm Based on Continuous Wavelet Transform and Investigating Its Effect on Improving Neural Decoding Accuracy.

Neuroscience 2021 Jun 5. Epub 2021 Jun 5.

Assistant Professor, Neuroscience and Neuroengineering Research Laboratory, Iran University of Science and Technology (IUST), Tehran, Iran. Electronic address:

Spike sorting is an essential step in extracting neuronal discharge patterns which help to decode different activities in the neural system. Therefore, improving the spike sorting accuracy can improve neural decoding performance subsequently. Although many methods are suggested for spike sorting, few studies have evaluated the effect of them on neural decoding performance. Read More

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Potential G-quadruplexes and i-Motifs in the SARS-CoV-2.

PLoS One 2021 8;16(6):e0250654. Epub 2021 Jun 8.

Advanced (Magnetic) Theranostic Nanostructures Lab, INL-International Iberian Nanotechnology Laboratory, Braga, Portugal.

Quadruplex structures have been identified in a plethora of organisms where they play important functions in the regulation of molecular processes, and hence have been proposed as therapeutic targets for many diseases. In this paper we report the extensive bioinformatic analysis of the SARS-CoV-2 genome and related viruses using an upgraded version of the open-source algorithm G4-iM Grinder. This version improves the functionality of the software, including an easy way to determine the potential biological features affected by the candidates found. Read More

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Impact of errors in spot size and spot position in robustly optimized pencil beam scanning proton-based stereotactic body radiation therapy (SBRT) lung plans.

J Appl Clin Med Phys 2021 Jun 7. Epub 2021 Jun 7.

Centre for Medical Radiation Physics (CMRP), University of Wollongong, Wollongong, New South Wales, Australia.

Purpose: The purpose of the current study was threefold: (a) investigate the impact of the variations (errors) in spot sizes in robustly optimized pencil beam scanning (PBS) proton-based stereotactic body radiation therapy (SBRT) lung plans, (b) evaluate the impact of spot sizes and position errors simultaneously, and (c) assess the overall effect of spot size and position errors occurring simultaneously in conjunction with either setup or range errors.

Methods: In this retrospective study, computed tomography (CT) data set of five lung patients was selected. Treatment plans were regenerated for a total dose of 5000 cGy(RBE) in 5 fractions using a single-field optimization (SFO) technique. Read More

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Reconstruction and catalytic activity of hybrid Pd(100)/(111) monolayer on γ-AlO(100) in CH, HO, and O dissociation.

Dalton Trans 2021 Jun 8. Epub 2021 Jun 8.

Department of Chemistry, Moscow State University, GSP-2, Leninskie Gory, Moscow 119992, Russia.

The importance of the "heterogeneity" of a Pd monolayer induced by interaction with a semi-ionic support in catalysis was evaluated. The geometry of the Pd monolayer was optimized on the (100) plane of γ-Al2O3 at fixed unit cell parameters defined by the oxide. Simulation of the deposition of a whole Pd monolayer in the flat Pd(100) form cut from the bulk led to the formation of a slightly distorted Pd(111) monolayer. Read More

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Preclinical immunogenicity testing using anti-drug antibody analysis of GX-G3, Fc-fused recombinant human granulocyte colony-stimulating factor, in rat and monkey models.

Sci Rep 2021 Jun 7;11(1):12004. Epub 2021 Jun 7.

Bioanalytical and Immunoanalytical Research Group, Department of Advanced Toxicology Research, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, Daejeon, 34114, Republic of Korea.

Human granulocyte colony-stimulating factor (G-CSF, this study used Fc-fused recombinant G-CSF; GX-G3) is an important glycoprotein that stimulates the proliferation of granulocytes and white blood cells. Thus, G-CSF treatment has been considered as a crucial regimen to accelerate recovery from chemotherapy-induced neutropenia in cancer patients suffering from non-myeloid malignancy or acute myeloid leukemia. Despite the therapeutic advantages of G-CSF treatment, an assessment of its immunogenicity must be performed to determine whether the production of anti-G-CSF antibodies causes immune-related disorders. Read More

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Techno-economic evaluation of microalgae high-density liquid fuel production at 12 international locations.

Biotechnol Biofuels 2021 Jun 7;14(1):133. Epub 2021 Jun 7.

Institute for Molecular Bioscience, The University of Queensland, 306 Carmody Road, Brisbane, QLD, 4072, Australia.

Background: Microalgae-based high-density fuels offer an efficient and environmental pathway towards decarbonization of the transport sector and could be produced as part of a globally distributed network without competing with food systems for arable land. Variations in climatic and economic conditions significantly impact the economic feasibility and productivity of such fuel systems, requiring harmonized technoeconomic assessments to identify important conditions required for commercial scale up.

Methods: Here, our previously validated Techno-economic and Lifecycle Analysis (TELCA) platform was extended to provide a direct performance comparison of microalgae diesel production at 12 international locations with variable climatic and economic settings. Read More

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Lesion segmentation in breast ultrasound images using the optimized marked watershed method.

Biomed Eng Online 2021 Jun 7;20(1):57. Epub 2021 Jun 7.

College of Medicine and Biological Information Engineering, Northeastern University, Shenyang, China.

Background: Breast cancer is one of the most serious diseases threatening women's health. Early screening based on ultrasound can help to detect and treat tumours in the early stage. However, due to the lack of radiologists with professional skills, ultrasound-based breast cancer screening has not been widely used in rural areas. Read More

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Cationic lipid nanoparticle production by microfluidization for siRNA delivery.

Int J Pharm 2021 Jun 4:120772. Epub 2021 Jun 4.

Université de Paris, UTCBS, CNRS, INSERM, F-75006 Paris, France. Electronic address:

Microfluidization has been investigated as a new, scalable, and basic component saving method to produce cationic lipid nanoparticles, in particular for the delivery of short interfering RNAs (siRNAs). The design of experiment (DoE) allowed to reach optimized characteristics in terms of nanocarrier size reduction and low polydispersity. The structure of cationic liposomes and siRNA-lipoplexes was characterized. Read More

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Defocus Blur Detection via Boosting Diversity of Deep Ensemble Networks.

IEEE Trans Image Process 2021 8;30:5426-5438. Epub 2021 Jun 8.

Existing defocus blur detection (DBD) methods usually explore multi-scale and multi-level features to improve performance. However, defocus blur regions normally have incomplete semantic information, which will reduce DBD's performance if it can't be used properly. In this paper, we address the above problem by exploring deep ensemble networks, where we boost diversity of defocus blur detectors to force the network to generate diverse results that some rely more on high-level semantic information while some ones rely more on low-level information. Read More

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Comprehensive echocardiographic evaluation of the right heart in patients with pulmonary vascular diseases: the PVDOMICS experience.

Eur Heart J Cardiovasc Imaging 2021 Jun 7. Epub 2021 Jun 7.

Bluhm Cardiovascular Institute, Northwestern University, 676 N Saint Clair, Chicago Illinois 60611 USA.

Aims: There is a wide spectrum of diseases associated with pulmonary hypertension, pulmonary vascular remodelling, and right ventricular dysfunction. The NIH-sponsored PVDOMICS network seeks to perform comprehensive clinical phenotyping and endophenotyping across these disorders to further evaluate and define pulmonary vascular disease.

Methods And Results: Echocardiography represents the primary non-invasive method to phenotype cardiac anatomy, function, and haemodynamics in these complex patients. Read More

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Development of a high-throughput device for liquid-phase microextraction coupled with gas chromatography-mass spectrometry for the determination of benzene derivatives in environmental water.

Anal Methods 2021 Jun 7. Epub 2021 Jun 7.

College of Chemistry and Materials Science, Northwest University, Xi'an 710127, China.

A device coupled with an automatic gas chromatography injection sampler for high-throughput liquid-liquid microextraction has been proposed in this study. This study's main objective was to devise a trap vial directly placed in an automatic liquid sampler (ALS) to collect and inject the extractant without a transfer step. Sixteen groups of microextraction procedures were performed simultaneously to increase the overall efficiency. Read More

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An Artificial Intelligence-Based Model for Performance Prediction of Acid Fracturing in Naturally Fractured Reservoirs.

ACS Omega 2021 Jun 18;6(21):13654-13670. Epub 2021 May 18.

College of Petroleum Engineering & Geosciences, King Fahd University of Petroleum & Minerals, Dhahran 31261, Saudi Arabia.

Acid fracturing is one of the most effective techniques for improving the productivity of naturally fractured carbonate reservoirs. Natural fractures (NFs) significantly affect the design and performance of acid fracturing treatments. However, few models have considered the impact of NFs on acid fracturing treatments. Read More

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Density-functional tight-binding for phosphine-stabilized nanoscale gold clusters.

Chem Sci 2020 Nov 2;11(48):13113-13128. Epub 2020 Nov 2.

Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee Knoxville TN USA.

We report a parameterization of the second-order density-functional tight-binding (DFTB2) method for the quantum chemical simulation of phosphine-ligated nanoscale gold clusters, metalloids, and gold surfaces. Our parameterization extends the previously released DFTB2 "auorg" parameter set by connecting it to the electronic parameter of phosphorus in the "mio" parameter set. Although this connection could technically simply be accomplished by creating only the required additional Au-P repulsive potential, we found that the Au 6p and P 3d virtual atomic orbital energy levels exert a strong influence on the overall performance of the combined parameter set. Read More

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November 2020

Photoresponsive molecular tools for emerging applications of light in medicine.

Chem Sci 2020 Oct 15;11(43):11672-11691. Epub 2020 Oct 15.

Department of Radiology, Medical Imaging Center, University Medical Center Groningen Groningen The Netherlands

Light-based therapeutic and imaging modalities, which emerge in clinical applications, rely on molecular tools, such as photocleavable protecting groups and photoswitches that respond to photonic stimulus and translate it into a biological effect. However, optimisation of their key parameters (activation wavelength, band separation, fatigue resistance and half-life) is necessary to enable application in the medical field. In this perspective, we describe the applications scenarios that can be envisioned in clinical practice and then we use those scenarios to explain the necessary properties that the photoresponsive tools used to control biological function should possess, highlighted by examples from medical imaging, drug delivery and photopharmacology. Read More

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October 2020

Sensitivity analysis of a multibranched light guide for real time hyperspectral imaging systems.

Proc SPIE Int Soc Opt Eng 2019 Feb 27;10871. Epub 2019 Feb 27.

Chemical and Biomolecular Engineering, University of South Alabama, AL 36688.

Hyperspectral imaging (HSI) is a spectroscopic technique which captures images at a high contrast over a wide range of wavelengths to show pixel specific composition. Traditional uses of HSI include: satellite imagery, food distribution quality control and digital archaeological reconstruction. Our lab has focused on developing applications of HSI fluorescence imaging systems to study molecule-specific detection for rapid cell signaling events or real-time endoscopic screening. Read More

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February 2019