7,507 results match your criteria opportunistic pathogens


New Insight To The Rol Of Α-Enolase (Eno-1) As Immunological Marker In Rainbow Trout Fry.

Dev Comp Immunol 2021 Jun 9:104163. Epub 2021 Jun 9.

Grupo de Marcadores Inmunológicos, Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Avenida Universidad #330, 2373223, Valparaíso, Chile; Núcleo Biotecnológico de Curauma (NBC), Pontificia Universidad Católica de Valparaíso, Avenida Universidad #330, 2373223, Valparaíso, Chile. Electronic address:

α-Enolase is an enzyme of the glycolytic pathway that has also been involved in vertebrate inflammatory processes through its interaction with plasminogen. However, its participation in the immune response of lower vertebrates during early life development is unknown. Opportunistic pathogens in salmon farming are the principal cause of mortality in the fry stage. Read More

View Article and Full-Text PDF

An Inventory of CiaR-Dependent Small Regulatory RNAs in .

Front Microbiol 2021 25;12:669396. Epub 2021 May 25.

Université de Tours, INRAE, ISP, Tours, France.

Bacteria adapt to the different environments encountered by rapid and tightly controlled regulations involving complex networks. A first line of control is transcriptional with regulators such as two-component systems (TCSs) that respond to physical and chemical perturbations. It is followed by posttranscriptional regulations in which small regulatory RNAs (sRNAs) may affect RNA translation. Read More

View Article and Full-Text PDF

Broad-Spectrum Photo-Antimicrobial Polymers Based on Cationic Polystyrene and Rose Bengal.

Front Med (Lausanne) 2021 24;8:641646. Epub 2021 May 24.

Departamento de Química Inorgánica y Orgánica, Universitat Jaume I, Castellón, Spain.

New strategies to fight bacteria and fungi are necessary in view of the problem of iatrogenic and nosocomial infections combined with the growing threat of increased antimicrobial resistance. Recently, our group has prepared and described two new readily available materials based on the combination of Rose Bengal (singlet oxygen photosensitizer) and commercially available cationic polystyrene (macroporous resin Amberlite® IRA 900 or gel-type resin IRA 400). These materials showed high efficacy in the antimicrobial photodynamic inactivation (aPDI) of . Read More

View Article and Full-Text PDF

Genomic and Phenotypic Analysis of COVID-19-Associated Pulmonary Aspergillosis Isolates of Aspergillus fumigatus.

Microbiol Spectr 2021 Jun 9:e0001021. Epub 2021 Jun 9.

Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, USA.

The ongoing global pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for coronavirus disease 2019 (COVID-19), first described in Wuhan, China. A subset of COVID-19 patients has been reported to have acquired secondary infections by microbial pathogens, such as opportunistic fungal pathogens from the genus Aspergillus. To gain insight into COVID-19-associated pulmonary aspergillosis (CAPA), we analyzed the genomes and characterized the phenotypic profiles of four CAPA isolates of Aspergillus fumigatus obtained from patients treated in the area of North Rhine-Westphalia, Germany. Read More

View Article and Full-Text PDF

Phylogenomic Characterization of a Novel Species Associated with Fatal Diphtheritic Stomatitis in Endangered Yellow-Eyed Penguins.

mSystems 2021 Jun 8:e0032021. Epub 2021 Jun 8.

Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, United Kingdom.

Yellow-eyed penguins, Megadyptes antipodes, are an endangered species that are endemic to New Zealand. Outbreaks of diphtheritic stomatitis have caused significant mortality for this species, especially among young chicks. In this study, we isolated 16 sp. Read More

View Article and Full-Text PDF

Stochastic processes shape the bacterial community assembly in shrimp cultural pond sediments.

Appl Microbiol Biotechnol 2021 Jun 7. Epub 2021 Jun 7.

State Key Laboratory of Biocontrol/Southern Marine Sciences and Engineering Guangdong Laboratory (Zhuhai)/School of Marine Sciences, Sun Yat-sen University, Guangzhou, 510275, People's Republic of China.

Sediment environments harbor a repertoire of microorganisms that contribute to animal health and the microecosystem in aquaculture ecosystems, but their community diversity and the potential factors that control it remain unclear. Here, we applied 16S rRNA gene amplicon sequencing to investigate bacterial diversity and assembly mechanisms in the sediments of shrimp cultural ponds at the mesoscale. Our results showed that sediment bacterial communities contained 10,333 operational taxonomic units (OTUs) but had only 34 core OTUs and that the relative abundances of these core OTUs were significantly correlated with the physicochemical properties of the sediments. Read More

View Article and Full-Text PDF

Destination shapes antibiotic resistance gene acquisitions, abundance increases, and diversity changes in Dutch travelers.

Genome Med 2021 Jun 7;13(1):79. Epub 2021 Jun 7.

Department of Medical Microbiology, Care and Public Health Research Institute (CAPHRI), Maastricht University Medical Center, Maastricht, The Netherlands.

Background: Antimicrobial-resistant bacteria and their antimicrobial resistance (AMR) genes can spread by hitchhiking in human guts. International travel can exacerbate this public health threat when travelers acquire AMR genes endemic to their destinations and bring them back to their home countries. Prior studies have demonstrated travel-related acquisition of specific opportunistic pathogens and AMR genes, but the extent and magnitude of travel's effects on the gut resistome remain largely unknown. Read More

View Article and Full-Text PDF

Occurrence of major and minor pathogens in calves diagnosed with bovine respiratory disease.

Vet Microbiol 2021 May 27;259:109135. Epub 2021 May 27.

Department of Veterinary and Animal Science University of Copenhagen, Copenhagen, Denmark. Electronic address:

Bovine respiratory disease (BRD) is caused by a mixture of viruses and opportunistic bacteria belonging to Pasteurellaceae and Mycoplasma bovis. However, these organisms are also commonly isolated from healthy calves. This study aimed to determine whether the organisms are present in higher numbers in calves sick with acute BRD than in clinically healthy calves, and further to genetically characterize bacteria of the family Pasteurellaceae to understand whether particular types are associated with disease. Read More

View Article and Full-Text PDF

Occurrence and removal of antibiotics, antibiotic resistance genes, and bacterial communities in hospital wastewater.

Environ Sci Pollut Res Int 2021 Jun 4. Epub 2021 Jun 4.

State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, China.

Hospital wastewater contains a variety of human antibiotics and pathogens, which makes the treatment of hospital wastewater essential. However, there is a lack of research on these pollutants at hospital wastewater treatment plants. In this study, the characteristics and removal of antibiotics and antibiotic resistance genes (ARGs) in the independent treatment processes of hospitals of different scales (primary hospital, H1; secondary hospital, H2; and tertiary hospital, H3) were investigated. Read More

View Article and Full-Text PDF

The devil is in the validation and design; managing the risk from opportunistic pathogens in the dental unit.

Authors:
Andrew Smith

J Hosp Infect 2021 Jun 1. Epub 2021 Jun 1.

College of Medical, Veterinary & Life Sciences, Glasgow Dental Hospital & School, University of Glasgow, 378 Sauchiehall Street, Glasgow G2 3JZ. Electronic address:

View Article and Full-Text PDF

Opinion The truth is in the eye of the beholder; opportunistic pathogens in the dental unit.

J Hosp Infect 2021 Jun 1. Epub 2021 Jun 1.

Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.

View Article and Full-Text PDF

Urinary Microbiome: Yin and Yang of the Urinary Tract.

Front Cell Infect Microbiol 2021 18;11:617002. Epub 2021 May 18.

GENYO, Centre for Genomics and Oncological Research, Pfizer, University of Granada, Granada, Spain.

The application of next generation sequencing techniques has allowed the characterization of the urinary tract microbiome and has led to the rejection of the pre-established concept of sterility in the urinary bladder. Not only have microbial communities in the urinary tract been implicated in the maintenance of health but alterations in their composition have also been associated with different urinary pathologies, such as urinary tract infections (UTI). Therefore, the study of the urinary microbiome in healthy individuals, as well as its involvement in disease through the proliferation of opportunistic pathogens, could open a potential field of study, leading to new insights into prevention, diagnosis and treatment strategies for urinary pathologies. Read More

View Article and Full-Text PDF

Solvent Dependency of Sorghum Bran Phytochemicals Acting as Potential Antioxidants and Antibacterial Agents.

Food Technol Biotechnol 2021 Mar;59(1):31-43

Department of Basic and Applies Sciences, National Institute of Food Technology Entrepreneurship and Management, Sector - 56, HSIIDC Industrial Estate, Kundli, Sonipat, 131028 Haryana, India.

Research Background: Sorghum bran, although considered as an agricultural waste, is an abundant source of various bioactive compounds. These bioactive compounds require specific extraction with particular solvents and therefore ionic liquid and three different conventional solvents, anhydrous methanol, acidified methanol and water were used in this work.

Experimental Approach: To evaluate the phytochemicals in the different sorghum bran extracts, total phenol content, flavonoids, condensed tannins and anthocyanins were determined as per standard protocols. Read More

View Article and Full-Text PDF

Engineering Gac/Rsm Signaling Cascade for Optogenetic Induction of the Pathogenicity Switch in .

ACS Synth Biol 2021 Jun 2. Epub 2021 Jun 2.

Hefei National Laboratory for Physical Sciences at the Microscale; Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, 230026, China.

Bacterial pathogens operate by tightly controlling the pathogenicity to facilitate invasion and survival in host. While small molecule inducers can be designed to modulate pathogenicity to perform studies of pathogen-host interaction, these approaches, due to the diffusion property of chemicals, may have unintended, or pleiotropic effects that can impose limitations on their use. By contrast, light provides superior spatial and temporal resolution. Read More

View Article and Full-Text PDF

Intricate Connections between the Microbiota and Endometriosis.

Int J Mol Sci 2021 May 26;22(11). Epub 2021 May 26.

Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of British Columbia, D415A-4500 Oak Street, Vancouver, BC V6H 3N1, Canada.

Imbalances in gut and reproductive tract microbiota composition, known as dysbiosis, disrupt normal immune function, leading to the elevation of proinflammatory cytokines, compromised immunosurveillance and altered immune cell profiles, all of which may contribute to the pathogenesis of endometriosis. Over time, this immune dysregulation can progress into a chronic state of inflammation, creating an environment conducive to increased adhesion and angiogenesis, which may drive the vicious cycle of endometriosis onset and progression. Recent studies have demonstrated both the ability of endometriosis to induce microbiota changes, and the ability of antibiotics to treat endometriosis. Read More

View Article and Full-Text PDF

Biomaterials for the Prevention of Oral Candidiasis Development.

Pharmaceutics 2021 May 27;13(6). Epub 2021 May 27.

Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 060042 Bucharest, Romania.

Thousands of microorganisms coexist within the human microbiota. However, certain conditions can predispose the organism to the overgrowth of specific pathogens that further lead to opportunistic infections. One of the most common such imbalances in the normal oral flora is the excessive growth of spp. Read More

View Article and Full-Text PDF

SARS-CoV-2-Induced Gut Microbiome Dysbiosis: Implications for Colorectal Cancer.

Cancers (Basel) 2021 May 28;13(11). Epub 2021 May 28.

James A. Haley Veterans Hospital, Department of Veterans Affairs, Tampa, FL 33612, USA.

The emergence of a novel coronavirus, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), in December 2019 led to a worldwide pandemic with over 170 million confirmed infections and over 3.5 million deaths (as of May 2021). Early studies have shown higher mortality rates from SARS-CoV-2 infection in cancer patients than individuals without cancer. Read More

View Article and Full-Text PDF

In Silico Prediction and Prioritization of Novel Selective Antimicrobial Drug Targets in .

Antibiotics (Basel) 2021 May 25;10(6). Epub 2021 May 25.

Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK 2000 Frederiksberg, Denmark.

Novel antimicrobials interfering with pathogen-specific targets can minimize the risk of perturbations of the gut microbiota (dysbiosis) during therapy. We employed an in silico approach to identify essential proteins in that are either absent or have low sequence identity in seven beneficial taxa of the gut microbiota: , , , , , and . We identified 36 essential proteins that are present in hyper-virulent ST131 and have low similarity (bitscore < 50 or identity < 30% and alignment length < 25%) to proteins in mammalian hosts and beneficial taxa. Read More

View Article and Full-Text PDF

Lactic Starter Dose Shapes and STEC O26:H11 Growth, and Bacterial Community Patterns in Raw Milk Uncooked Pressed Cheeses.

Microorganisms 2021 May 18;9(5). Epub 2021 May 18.

UMR 0545 Fromage, Université Clermont Auvergne, INRAE, VetAgro Sup, 20 Côte de Reyne, F-15000 Aurillac, France.

Adding massive amounts of lactic starters to raw milk to manage the sanitary risk in the cheese-making process could be detrimental to microbial diversity. Adjusting the amount of the lactic starter used could be a key to manage these adverse impacts. In uncooked pressed cheeses, we investigated the impacts of varying the doses of a lactic starter (the recommended one, 1×, a 0. Read More

View Article and Full-Text PDF

An Antisense RNA Modulates Biofilm Organization of Methicillin-Resistant and Pathogenicity in a Rat Model of Osteomyelitis.

Antibiotics (Basel) 2021 May 19;10(5). Epub 2021 May 19.

Department of Orthopedics, West China Hospital, Sichuan University, Chengdu 610041, China.

() is one of most common opportunistic pathogens and is attributed to several human infections. The increasing incidence of methicillin-resistant (MRSA) is a serious clinical threat for osteomyelitis crisis. The YycFG two-component system of regulates genes associated with biofilm formation. Read More

View Article and Full-Text PDF

Phage Biocontrol of in Water.

Viruses 2021 05 17;13(5). Epub 2021 May 17.

Expert Microbiology Unit, Department of Health Security, Finnish Institute for Health and Welfare, FI-70701 Kuopio, Finland.

Bacteriophage control of harmful or pathogenic bacteria has aroused growing interest, largely due to the rise of antibiotic resistance. The objective of this study was to test phages as potential agents for the biocontrol of an opportunistic pathogen in water. Two bacteriophages (vB_PaeM_V523 and vB_PaeM_V524) were isolated from wastewater and characterized physically and functionally. Read More

View Article and Full-Text PDF

The Synthesis and Evaluation of Multivalent Glycopeptoids as Inhibitors of the Adhesion of .

Pathogens 2021 May 8;10(5). Epub 2021 May 8.

Department of Chemistry, Maynooth University, Maynooth, W23VP22 Co. Kildare, Ireland.

Multivalency is a strategy commonly used by medicinal carbohydrate chemists to increase the affinity of carbohydrate-based small molecules for their protein targets. Although this approach has been very successful in enhancing binding to isolated carbohydrate-binding proteins, anticipating the multivalent presentations that will improve biological activity in cellular assays remains challenging. In this work we investigate linear molecular scaffolds for the synthesis of a low valency presentation of a divalent galactoside , previously identified by us as an inhibitor of the adhesion of opportunistic fungal pathogen to buccal epithelial cells (BECs). Read More

View Article and Full-Text PDF

Elevated Free Phosphatidylcholine Levels in Cerebrospinal Fluid Distinguish Bacterial from Viral CNS Infections.

Cells 2021 May 6;10(5). Epub 2021 May 6.

Research Group Biomarkers for Infectious Diseases, TWINCORE Centre for Experimental and Clinical Infection Research, 30625 Hannover and Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany.

The identification of CSF biomarkers for bacterial meningitis can potentially improve diagnosis and understanding of pathogenesis, and the differentiation from viral CNS infections is of particular clinical importance. Considering that substantial changes in CSF metabolites in CNS infections have recently been demonstrated, we compared concentrations of 188 metabolites in CSF samples from patients with bacterial meningitis ( = 32), viral meningitis/encephalitis ( = 34), and noninflamed controls ( = 66). Metabolite reprogramming in bacterial meningitis was greatest among phosphatidylcholines, and concentrations of all 54 phosphatidylcholines were significantly ( = 1. Read More

View Article and Full-Text PDF

A Nosocomial Outbreak of Invasive Listeriosis in An Italian Hospital: Epidemiological and Genomic Features.

Pathogens 2021 May 12;10(5). Epub 2021 May 12.

Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "M. Aleandri"-Sezione di Roma, 00178 Rome, Italy.

() is a widespread opportunistic pathogen that causes the listeriosis foodborne disease. This bacterium has become a common contaminant of handled food, and a relevant public health issue. Here we describe a nosocomial outbreak of listeriosis caused by an ST451 strain of involving three cancer and one immunocompromised patients hospitalized in different units from the same hospital during September and October 2020. Read More

View Article and Full-Text PDF

Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles.

Molecules 2021 May 11;26(10). Epub 2021 May 11.

Departamento de Química Inorgánica, Universidad de Granada, 18071 Granada, Spain.

The constant increase of antibiotic-resistant bacteria demands the design of novel antibiotic-free materials. The combination of antibacterials in a biocompatible biomaterial is a very promising strategy to treat infections caused by a broader spectrum of resistant pathogens. Here, we combined two antibacterials, silver nanoparticles (AgNPs) and living probiotics (, ), using bacterial cellulose (BC) as scaffold. Read More

View Article and Full-Text PDF

Identification of Genes Specifically Expressed under Simulated Human Digestive Conditions Using R-IVET Technology.

Microorganisms 2021 May 21;9(6). Epub 2021 May 21.

EA 7488 Calbinotox Composés Alimentaires Biofonctionnalités & Risque Neurotoxique, Université de Lorraine, 54506 Vandoeuvre-lès-Nancy, France.

Despite promising health effects, the probiotic status of a lactic acid bacterium widely used in dairy industry, requires further documentation of its physiological status during human gastrointestinal passage. This study aimed to apply recombinant-based in vivo technology (R-IVET) to identify genes triggered in a LMD-9 reference strain under simulated digestive conditions. First, the R-IVET chromosomal cassette and plasmid genomic library were designed to positively select activated genes. Read More

View Article and Full-Text PDF

Antimicrobial Photodynamic Inactivation Affects the Antibiotic Susceptibility of spp. Clinical Isolates in Biofilm and Planktonic Cultures.

Biomolecules 2021 May 5;11(5). Epub 2021 May 5.

Laboratory of Molecular Diagnostics, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, 80-307 Gdansk, Poland.

and are opportunistic pathogens that can cause a vast variety of nosocomial infections. Moreover, belongs to the group of ESKAPE microbes, which are the main cause of hospital-acquired infections and are especially difficult to treat because of their resistance to many antibiotics. Antimicrobial photodynamic inactivation (aPDI) represents an alternative to overcome multidrug resistance problems. Read More

View Article and Full-Text PDF

Precise Species Identification for Acinetobacter: a Genome-Based Study with Description of Two Novel Acinetobacter Species.

mSystems 2021 May 26:e0023721. Epub 2021 May 26.

Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

The genus Acinetobacter comprises species with ecological significance and opportunistic pathogens and has a complicated taxonomy. Precise species identification is a foundation for understanding bacteria. In this study, we found and characterized two novel Acinetobacter species, namely, Acinetobacter tianfuensis sp. Read More

View Article and Full-Text PDF

The microbiological load and microbiome of the Dutch dental unit; 'please, hold your breath'.

Water Res 2021 May 6;200:117205. Epub 2021 May 6.

Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.

Dental unit water systems are prone to biofilm formation. During use of the dental unit, clumps of biofilm slough off and can subsequently be aerosolized and inhaled by both patient and staff, potentially causing infections. The aim of this study was to determine the microbial load and microbiome of dental unit water, in the Netherlands, and the factors influencing these parameters. Read More

View Article and Full-Text PDF

Tacrolimus exposure windows responsible for Listeria monocytogenes infection susceptibility.

Transpl Infect Dis 2021 May 31:e13655. Epub 2021 May 31.

Division of Infectious Diseases, Center for Inflammation and Tolerance, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, 45229, USA.

Tacrolimus is widely used to prevent graft rejection after allogeneic transplantation by suppressing T cells in a non-antigen-specific fashion. Global T cell suppression makes transplant recipients more susceptible to infection, especially infection by opportunistic intracellular pathogens. Infection followed by secondary challenge with the opportunistic intracellular bacterial pathogen, Listeria monocytogenes, was used to probe when tacrolimus most significantly impacts antimicrobial host defense. Read More

View Article and Full-Text PDF